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Histopathology Unit: Pretest

The document provides an overview of the equipment and processes used in a histopathology unit, including an automatic tissue processor, embedding center, rotary microtome, freezing microtome, dry heat oven, automatic tissue stainer, centrifuge, cytospin, and immunohistochemical stains. Specimens are processed through tissue processing, staining, and examination under slides by a pathologist to examine for cancer. A variety of diagnostic services are offered including routine histopathology, rush frozen sections, cytopathology, and immunohistochemistry.

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Mary Cabalce
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426 views3 pages

Histopathology Unit: Pretest

The document provides an overview of the equipment and processes used in a histopathology unit, including an automatic tissue processor, embedding center, rotary microtome, freezing microtome, dry heat oven, automatic tissue stainer, centrifuge, cytospin, and immunohistochemical stains. Specimens are processed through tissue processing, staining, and examination under slides by a pathologist to examine for cancer. A variety of diagnostic services are offered including routine histopathology, rush frozen sections, cytopathology, and immunohistochemistry.

Uploaded by

Mary Cabalce
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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HISTOPATHOLOGY UNIT

PRETEST
Automatic Tissue Processor A machine that prepares tissues for sectioning thru series of
(Leica TP1020) alcohol, clearing agent and embedding medium.

Embedding Center After 16 hours in the automatic tissue processor, tissues are
(Histostar) then brought to the embedding center for embedding, it
composts of a hot plate, paraffin dispenser and cold plate.

Tissue Molds Where tissues are placed properly so that it can be easily
sectioned using a rotary microtome.

Rotary Microtome Used to produce thin sections ranging from 4 to 6 um


(Microtec)

Freezing Microtome (Cryostat) Used when Rush Frozen Section is requested, fresh unfixed
tissues are placed under extreme cold temperature, using
cryomatrix instead of paraffin to hold the tissue when its being
cut. Diagnosis should be done around 15 minutes or less.

Dry heat Oven After placing the tissue sections on the slide its placed inside
the dry heat oven to facilitate initial deparaffinization and final
fixation.

Automatic Tissue Where the staining takes place whether routine hematoxylin
Stainer (Varistain) eosin staining or papaniculau staining procedure.

Centrifuge Equipment needed to produce cell block and pull apart smears
from fluid specimens for fluid cytology.

Cytospin Usually used for clear pathologic fluid, an example of


cerebrospinal fluid so than the cells are concentrated in an
area readily seen by the pathologists.

Immunohistochemical Stains One of the advancements in histopathology, aids physicians


on how they treat cancer patients by knowing the primary
cause of malignancies and how they can block it thru proper
medications.
INTRODUCTION

Ang histopathology section ay nakakatulong sa Service Offered:


pagsiyasat ukol sa cancer. Dito, ipinadadala ang • Pap Smear
biopsy na mula sa iba’t ibang bahagi ng katawan. • Histopathology
Ang specimens na ito ay dadaan sa tissue • Routine
processing, stainig atsaka ilalagay sa slides upang • Rush Frozen Section (RFS)
masuri ng isang pathologist.Sa histopathology unit • Cytopathology
din isinasagawa ang otopsiya. •Immunohistochemistry
• ER
Rooms: • CD-20
• Histopathology Unit • PR
• Staining Room • CD-7
• Gross Room • HER 2 NEU

Automated Tissue Processing For cutting ribbons of tissue sample embedded in


paraffin

Machine used to maintain low cryogenic temperature of


tissue for rush frozen section.

LECTURE
Anatomic Pathology
Step 1. Upon receiveing the Step 2. Assign an accession
specimen, see to it that the number and also remember that
data is complete and the the official request form should be
specimen is properly labeled. used. Label the accession number
on the top of the body in the
specimen container. Put the
appropriate fixative for the tissue
types. Respectively, used 10%
buffered formalin for
tissue biopsy and for
cytopathology, 95%
ethanol.
Step 3. After brushing the specimen by the pathologist, Step 4. After brushing the specimen by the pathologist,
we load the specimen on a tissue cassette and put them we load the specimen in the tissue cassettes and put
in our automatic tissue processor. For cytopathology, them in our automatic tissue processor
note that we use 2 tube method so that we can obtain a
cell block and a pull apart smear from the fluid
specimen.

Step 5. Embedding the tissue on tissue molds is one Step 6. For routine tissue biopsies, we use rotary
way of making a tissue block from the samples. microtome for tissue sectioning. For rush sections, we
are using freezing methods

Step 7. Placing the tissue


section in the floatation bath
and putting them on a glass
slide by teasing

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