SL NEQAS (BGS) REPORT FORM (March 2023)

Sri Lanka National External Quality Assessment Scheme for Blood Group Serology
Quality Management Section, National Blood Center, Tel: (011)2105250-69
No. 555, Elvitigala Mawatha, Narahenpita, Colombo 5. (011)2369931 / 2369935
Fax: (011)2369939

HBB Code No. Code

*Please perform all tests on samples provided despite of results obtained in other tests. Any differences in
routine practice should be stated in the comments section

1. Samples Received on: Date: 03/15/2023

2. Samples Tested on: Date: 03/17/2023

3. Report returned on: Date: Click or tap to enter a date.

4. Quality of samples at the time of testing

Patient’s Cells Patient’s Serum Donor’s Cells
P1C P2C P3C P1S P2S P3S Dy Dz
Satisfactory ☒ ☒ ☒ ☒ ☒ ☒ ☒ ☒
Unsatisfactory ☐ ☐ ☐ ☐ ☐ ☐ ☐ ☐
(If unsatisfactory please mention the reason – e.g.: haemolysed / decomposed / leaking etc.)
Click or tap here to enter text.

5. Reagents used for testing

Product name & Manufacturer Lot No Exp.Date
Anti A (Poly cl ☐ / Mono cl☒ ) Immulab Vic Australia 026141301 12/15/2024
Anti AB (Poly cl ☐ / Mono cl☒ ) Immulab Vic Australia 026731601 08/26/2023
Anti B (Poly cl ☒ / Mono cl ☐ ) National Reagant laboratory SL 2302A 04/07/2023
Anti D1 (Poly cl ☐ / Mono cl ☒) Immulab Vic Australia 277210302 10/21/2023
Anti D2 (Poly cl ☐ / Mono cl ☐) Name and Manufacture Lot No date
Antibody Screening Cells (S1/S2/S3) NBTS 2302A 03/25/2023

AHG – Polyspecific (Anti-IgG/C3d) Immulab Vic Australia 030711701 03/23/2024

AHG – Monospecific (Anti-IgG) Immulab Vic Australia 025237601 10/01/2023

LISS (Additive ☒ / Suspension ☐) Immulab Vic Australia 010942101 01/15/2023

Albumin (Additive) Name and Manufacture Lot No date

Other : Name and Manufacture Lot No date

Name and Manufacture Lot No date

6. Techniques used in routine practice
6.1 ABO Grouping

Cell Grouping Only ☐ Cell & Serum Grouping ☒

Tube technique ☒ Column Agglutination ☐ Other: Click here to type

6.2 Rh D Grouping

Direct spin ☒ Direct spin + 370C ☐ Direct spin + 370C + IAT ☐

Tube technique ☒ Column technique ☐ Other: Click here to type

6.3 IAT techniques used

NISS ☐ LISS-Suspension ☐ LISS-Addition ☒ Albumin ☐

Tube technique ☒ Column technique ☐ Other: Click here to type

Remarks:
Click or tap here to enter text.

7. ABO & Rh D grouping

Please grade & record reaction results, in appropriate boxes)

Patient 1
ABO Cell grouping Rh D ABO Serum grouping
grouping INTERPRETATION OF
anti anti anti anti anti A1 B O ABO & Rh D group
A AB B D1 D2 cells cells cells
4+ 4+ 3+ 3+ selec 0 0 0 AB Positive
t.

Patient 2
ABO Cell grouping Rh D ABO Serum grouping
grouping INTERPRETATION OF
anti anti anti anti anti A1 B O ABO & Rh D group
A AB B D1 D2 cells cells cells
0 3+ 4+ 4+ selec 4+ 0 0 B Positive
t.

Patient 3
 ABO Cell grouping Rh D ABO Serum grouping
grouping INTERPRETATION OF
anti anti anti anti anti A1 B O ABO & Rh D group
A AB B D1 D2 cells cells cells
0 0 0 0 selec 3+ 3+ 0 O Negative
t.

Q1- Would you refer any of the above samples to the Reference IH laboratory before a non-
emergency transfusion? If yes, for what reason?
No

8. Direct Antiglobulin Test (DAT) (Please grade & record reaction results)

Result (Reaction
INTERPRETATION
strength)
Patient 1 0 Positive ☐ Negative ☒
Patient 2 0 Positive ☐ Negative ☒
Patient 3 1+ Positive ☒ Negative ☐

Q2- Would you refer any of the above samples to the Reference IH laboratory? If yes, for what
reason? What are the possibilities for your findings related to this sample?
No. Inform CTP. Do the DAT profile in the Blood bank.
autoimmune hemolytic anemia, hemolytic disease of the newborn, transfusion reactions

9. Screening for unexpected antibodies (Please grade and record reaction results)
(You may use 2 cell or 3 cell panel, as available for your routine practice)

Patient 1
37C IAT Other INTERPRETATION
Ab Screening Cell I (S1) 0 1+ select.
Ab detected ☒
Ab Screening Cell 2 (S2) 0 1+ select.
Ab not detected ☐
Ab Screening Cell 3 (S3) 0 0 select.

Patient 2
37C IAT Other INTERPRETATION
Ab Screening Cell I (S1) 0 1+ select.
Ab detected ☒
Ab Screening Cell 2 (S2) 0 1+ select.
Ab not detected ☐
Ab Screening Cell 3 (S3) 0 0 select.
 Patient 3
37C IAT Other INTERPRETATION
Ab Screening Cell I (S1) 0 0 select.
Ab Screening Cell 2 (S2) 0 0 select. Ab detected ☐
Ab not detected ☒
Ab Screening Cell 3 (S3) 0 0 select.

Q3- Would you refer any of the above samples to the Reference IH laboratory before a non-
emergency transfusion? If yes, for what reason? If in an emergency, how would you issue blood
for this patient?
Yes.
For antibody identification
In an emergency, issue uncrossmatched O negative blood and if time permits issue Group
specific IAT cross matched blood (LISS method)

10. Cross match (Please grade and record reaction results)

 Perform all 6 cross matches disregarding the ABO/Rh D groups of the patient/donor
samples
Patient 1
XM with D/Spin ( RT) 37C IAT Other INTERPRETATION
Compatible ☐
Donor Y 0 0 1+ select. Incompatible ☒
Compatible ☒
Donor Z 0 0 0 select. Incompatible ☐

Patient 2
XM with D/Spin (RT) 37C IAT Other INTERPRETATION
Compatible ☒
Donor Y 0 0 0 select. Incompatible ☐
Compatible ☒
Donor Z 0 0 0 select. Incompatible ☐

Patient 3
XM with D/Spin (RT) 37C IAT Other INTERPRETATION
Compatible ☐
Donor Y 3+ 2+ 3+ select. Incompatible ☒
Compatible ☒
Donor Z 0 0 0 select. Incompatible ☐
(RT = Room Temperature)
Q4- If any of the above shows incompatibility what could be the reason for it?
Donor Y- Blood group is B positive | Donor Z- Blood group is O negative.
P1/Donor Y- P1 is AB positive and Donor Y is B positive. So it can be a minor blood group
incompatibility ( antibodies in P1 sample clumping with donor cells)
P3/Donor Y- P3 is O negative and Donor Y is B positive, so it is a ABO blood group
incompatibility.
Please perform testing as early as possible
(Preferably within 3 days of receiving samples)
Original Report to be returned on or before 10/04/2023

Performed by

Signature

Name:Dr. VNRM Fonseka Date: 03/17/2023

MOIC/BB

Signature

Name:Click or tap here to enter text. Date: Click or tap to enter a date.

Telephone no: Click or tap here to enter text. Email address:Click or tap here to enter text.

Consultant /TP (if available)

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