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Protein Engineering Syllabus BIA1014

This document outlines a course on protein engineering that aims to impart advanced knowledge of protein functions, properties, and significance in biological systems. The course objectives are to analyze protein structure and construction through computer-based methods, describe protein structure and classification, and analyze protein sequence and structure relationships to function. The course outcomes are to design primers for PCR mutagenesis experiments and characterize proteins using techniques like mass spectroscopy, X-ray crystallography, and SDS-PAGE. The course contains 5 units that cover topics like primary and secondary protein structure, protein structure prediction, recombinant protein expression, and applications of protein engineering.

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Dhanalakshmi Saravanan
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301 views1 page

Protein Engineering Syllabus BIA1014

This document outlines a course on protein engineering that aims to impart advanced knowledge of protein functions, properties, and significance in biological systems. The course objectives are to analyze protein structure and construction through computer-based methods, describe protein structure and classification, and analyze protein sequence and structure relationships to function. The course outcomes are to design primers for PCR mutagenesis experiments and characterize proteins using techniques like mass spectroscopy, X-ray crystallography, and SDS-PAGE. The course contains 5 units that cover topics like primary and secondary protein structure, protein structure prediction, recombinant protein expression, and applications of protein engineering.

Uploaded by

Dhanalakshmi Saravanan
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIA10 PROTEIN ENGINEERING 3 0 2 4

Prerequisite BTA05
Course 1. The course imparts advanced knowledge on proteins through a detailed
Objectives study of their functions and properties.
2. To inculcate the role of protein structure, characteristics property and
significance in biological systems.

Course On successful completion of this course the students will be able to:
Outcomes 1. Analyse the structure and construction of proteins by computer-based
methods. (1)
2. Describe the structure and classification of proteins. (1)
3. Analyse and compare the amino acid sequence and structure of proteins,
and relate this information to the function of proteins. (1, 3)
4. Outline the characteristics of individual amino acids and their effect on the
solubility, structure and function of proteins. (1, 5)
5. Design primers to introduce mutations by means of PCR carry out a PCR-
based mutagenesis experiment. (5)
Note: Numbers given in the parenthesis refer to Graduate Attributes required by
NBA
UNIT I INTRODUCTION TO PROTEIN ENGINEERING 9
Introduction to Primary structure, Introduction to secondary structure, Introduction totertiary
structure, Introduction to quaternary structure, Ramachandran plots.
UNIT II PROTEIN STRUCTURE PREDICTION 9
Strategies for design of novel proteins-strategies for the design of structure and function,
computer methods in protein modelling
UNIT III PRODUCTION OF NOVEL PROTEINS 9
Site and strategies for heterologous expressions, methods for expressing recombinantproteins
in yeast, in vitro mutagenesis.
UNIT IV CHARACTERIZATION OF PROTEINS 9
Protein Characterization by Mass spectroscopy, X-RAY Crystallography, CD Spectra, UV Spectroscopy
and SDS Page.
UNIT V APPLICATIONS OF PROTEIN ENGINEERING 9
Design of polymeric biomaterials, nicotinic acetylcholine receptors as a model for a super family
of ligand - gated ion channel proteins
Text Books
1. Protein engineering and design by Paul R. Carey, academic press, 1996, 361 pages.
References
Yuriev, E.; Agostino, M. & Ramsland, PA. (2011). Challenges and advances in computational
docking: 2009 in review. Journal of Molecular Recognition: JMR, Vol. 24, No. 2, pp. 149- 164.
Zhang, H. (2002). Protein Tertiary Structures: Prediction from Amino Acid Sequences,
Encyclopedia of Life Sciences, Macmillan Publishers Ltd, Nature Publishing Group, England.

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