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DNA Worksheet

1. This document contains a 23 question worksheet about DNA replication. It asks students to define key terms related to DNA structure like nucleotides and their components. It also asks about early experiments that helped establish DNA as the genetic material like Griffith's experiments and Hershey-Chase. 2. The worksheet tests understanding of DNA replication, with questions about the unwinding of DNA, semi-conservative replication, the enzymes involved, and the direction DNA polymerase works. 3. It concludes by asking students to explain the importance of discovering DNA was the molecule of heredity.

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126 views2 pages

DNA Worksheet

1. This document contains a 23 question worksheet about DNA replication. It asks students to define key terms related to DNA structure like nucleotides and their components. It also asks about early experiments that helped establish DNA as the genetic material like Griffith's experiments and Hershey-Chase. 2. The worksheet tests understanding of DNA replication, with questions about the unwinding of DNA, semi-conservative replication, the enzymes involved, and the direction DNA polymerase works. 3. It concludes by asking students to explain the importance of discovering DNA was the molecule of heredity.

Uploaded by

H D
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Name Date Period

DNA & Replication Worksheet

1. Write out the complete name for DNA: __________________________________________

2. A nucleotide is made of three parts: a ___________________ group, a five carbon


__________________, and a nitrogen containing _____________________. Label
these three parts on the diagram to the right:

3. In a single strand of DNA, the phosphate group binds to the __________________


of the next group.

4. The 5' end of a single DNA strand contains a free __________________, while the 3' end contains a free
__________________________.

5. DNA was not thought to be the genetic blueprint originally; instead many scientists hypothesized that
_______________________ contained the genetic code and blueprint of life. (They were later proven
wrong.)

6. Purines have _________ rings, and pyrimidines have ____________ ring.

7. Check each of the following statements that are true with regard to Griffith's experiments.
______ Griffith discovered transformation in bacteria.
______ Mice injected with non-encapsulated bacteria quickly became sick and died.
______ Encapsulated bacteria can become "naked" bacteria.
______ Bacteria that are naked tend to be harmless.
______ Naked bacteria can develop a capsule only after exposure to the DNA of encapsulated bacteria.
______ Griffith's transforming factor was the protein in the capsule.
______ Heating the encapsulated bacteria preventing transformation of the naked variety.
______ Transformation allows bacteria to acquire new genes.
______ Enzymes that destroyed DNA would prevent transformation.

8. Check each of the following statements that are true with regard to the Hershey-Chase experiment.
_______ DNA was labeled with radioactive phosphorous.
_______ Protein was not labeled.
_______ Radioactive sulfur was found in the offspring phages after they emerged from the cell.
_______ Phage proteins were not transmitted to host cell or to offspring.

9. Chargaff's rule states that the DNA of any species contains equal amounts of __________________ &
____________ and also equal amounts of __________________ & ____________________

10. Wilkins and Franklin studied the structure of DNA using _________________________ and determined
that the shape of the molecule was a __________________

11. James Watson and Francis Crick amended Franklin and Wilkin's conclusions and determined that the shape of
the molecule was instead a _________________ __________________

12. Name the scientist(s) responsible for each of the following discoveries.
_____________________________________ Bacterial transformation
_____________________________________ The base-pair rule
_____________________________________ DNA was the hereditary material of viruses
_____________________________________ The shape of DNA was a helix
_____________________________________ The shape of DNA was a double helix

13. In DNA, thymine is complementary to ________________ ; cytosine is complementary to _____________


14. In a strand of DNA, the percentage of thymine is 30%. What is the percentage of cytosine in the same DNA
strand? _________________

15. The figure below shows DNA replicating. In the space provided, describe what is occurring.

16. Number the steps of DNA replication in the correct order (1, 2, 3)
_______ Daughter strands are formed using complementary base pairing.
_______ DNA unwinds
_______ The DNA of the daughter strands winds with together with its parent strand.

17. Why is DNA replication called "semi-conservative"? __________________________________________

18. What enzyme unwinds or unzips the parent strand? ________________

19. What enzyme binds fragments of DNA on the lagging strand? ______________________

20. DNA polymerase only travels in the ________ to _________ direction.

21. On the diagram:


Label the 3' and 5' ends.
Circle a nucleotide.
Label the sugar and phosphate.
Label the bases that are not already labeled

22. The two sides of the DNA helix are held together by
________________________

23. In your own words, explain why the discovery that DNA was
the molecule of heredity was an important discovery.

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