Edexcel (B) Biology A-level - DNA and Protein Synthesis

Questions
Q1.

Answer the question with a cross in the box you think is correct . If you change
your mind about an answer, put a line through the box and then mark your new
answer with a cross .

The diagram shows DNA replication.

Which of the following is the name of the bond labelled X on the diagram?
(1)
A ester
B glycosidic
C hydrogen
D phosphodiester

(Total for question = 1 mark)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q2.

The diagram shows DNA replication.

Describe the roles of three named enzymes involved in DNA replication.

(3)
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(Total for question = 3 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q3.

The diagram shows DNA replication.

A section of double-stranded DNA has a total number of 10 000 nucleotides.

Adenine makes up 35% of the nucleotides in this section of double-stranded DNA.

Calculate the total number of cytosine nucleotides found in this section of DNA.
(2)

Answer ...........................................................

(Total for question = 2 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q4.

Peptide hormones and steroid hormones are synthesised by endocrine glands.

Peptide hormones are transported dissolved in blood plasma. They bind to specific receptor
molecules on the cell surface membranes of their target organs.
Some peptide hormones are nonapeptides.
The table shows the amino acid sequences of three types of nonapeptide: A, B and C.

(i) Compare and contrast the amino acid sequence of these three nonapeptides.
(3)
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(ii) Explain why the DNA base sequences coding for these three nonapeptides are more
variable than their amino acid sequences.
(3)
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(Total for question = 6 marks)
Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q5.

There are three different types of haemoglobin in the blood of an adult human. Each
haemoglobin molecule is composed of four polypeptide chains.

The β, δ and γ polypeptide chains have similar amino acid sequences.

The table shows the sequence of nine amino acids in a part of each of these polypeptide
chains.

The β, δ and γ polypeptide chains are coded for by three different genes.

Compare and contrast the sequence of bases in the DNA coding for each of these parts of
the three polypeptide chains.
(4)
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(Total for question = 4 marks)
Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q6.

Answer the question with a cross in the box you think is correct . If you change
your mind about an answer, put a line through the box and then mark your new
answer with a cross .

The photographs show a black cat and a Siamese cat.

The pigment melanin causes the fur to turn black.

This pigment is produced by tyrosinase, an enzyme coded by the TYR gene.

(i) The diagram shows part of the DNA for the TYR gene.

Complete the diagram to show the messenger RNA produced by the

transcription of this DNA.
(1)

(ii) Some amino acids have more than one codon.

Which term describes this property of the genetic code?
(1)
A degenerate
B non-overlapping
C triplet code
D universal

(Total for question = 2 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q7.

The table shows the sequence of bases in part of an mRNA molecule.

Complete the table to show the base sequence of each of the following:

(i) the corresponding coding strand of DNA that produced this mRNA sequence
(1)
(ii) the base sequence of an antisense drug that will bind to this mRNA.
(1)

(iii) State the number of amino acids that would be coded for by the part of mRNA shown in
this table.
(1)
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(Total for question = 3 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q8.

A molecule of tRNA is made from a precursor molecule that is modified. Modification

includes splicing, trimming and attachment of new nucleotides.

The diagram shows a precursor molecule for a tRNA specific for the amino acid proline, and
a tRNA molecule specific for the amino acid proline. Some of the bases are shown in each
diagram.

(i) Which of the following base sequences is the mRNA code for proline?
(1)
A AAT
B CCA
C CCT
D CCU

(ii) Which of the following base sequences is the DNA code for proline?
(1)
A ACC
B CCA
C GGT
D UGG

(Total for question = 2 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q9.

A molecule of tRNA is made from a precursor molecule that is modified. Modification

includes splicing, trimming and attachment of new nucleotides.

The diagram shows a precursor molecule for a tRNA specific for the amino acid proline, and
a tRNA molecule specific for the amino acid proline. Some of the bases are shown in each
diagram.

Which bond is labelled Z?

(1)
A glycosidic
B hydrogen
C peptide
D phosphodiester

(Total for question = 1 mark)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q10.

Prothrombin is involved in the blood clotting process.

The F2 gene codes for the synthesis of prothrombin.

This gene is located from base pair 46 719 191 to base pair 46 739 504 on chromosome 11.

Determine the number of codons in this gene.

(1)

Answer ...........................................................

(Total for question = 1 mark)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q11.

Answer the question with a cross in the box you think is correct . If you change
your mind about an answer, put a line through the box and then mark your new
answer with a cross .

The nucleic acids, DNA and mRNA, are polymers.

The table shows some components that may be found in a molecule of these nucleic acids.

For each component, put one cross in the appropriate box, in each row, to show where
these components can be found.
(4)

(Total for question = 4 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q12.

The table shows the number of bases in the DNA strand used in the synthesis of mRNA.

Complete the table to show the percentage of bases in the mRNA synthesised.
(3)

(Total for question = 3 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q13.

Answer the question with a cross in the box you think is correct . If you change
your mind about an answer, put a line through the box and then mark your new
answer with a cross .

The graph shows the DNA content of a cell during four stages, I, II, III and IV, of one cell
cycle.

(i) In which stage does DNA replication take place?

(1)
A stage I
B stage II
C stage III
D stage IV

(ii) Explain the role of the enzyme ligase in DNA replication.

(3)
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(Total for question = 4 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q14.

Insulin is a protein that is composed of two polypeptide chains, chain A and chain B.

The diagram shows the amino acid sequence of these two polypeptide chains and some of
the bonds involved in the folding of this protein.

The table shows the DNA genetic codes on the anti-sense DNA strand (template strand) for
amino acids.
Edexcel (B) Biology A-level - DNA and Protein Synthesis

The genetic codes TAA, TAG and TGA are stop codons.

(i) Complete the diagram to show one base sequence in the antisense DNA strand
(template strand) coding for the five amino acids numbered in the diagram of insulin.
(1)

*(ii) Analyse the information to deduce how gene mutations in the DNA base sequence for
Cys (amino acid number 2) could affect the structure of insulin.
(6)
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(Total for question = 7 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q15.

Answer the question with a cross in the box you think is correct . If you change
your mind about an answer, put a line through the box and then mark your new
answer with a cross .

(i) Which bonds join the nucleotides in a single strand of DNA?

(1)
A peptide
B disulfide
C glycosidic
D phosphodiester

(ii) Describe how the process of DNA replication produces two identical DNA molecules.
(4)
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(Total for question = 5 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q16.

Eukaryotic cells and prokaryotic cells have similarities and differences in their ultrastructure.

Some antibiotics inhibit RNA synthesis and protein synthesis in cells.

Actinomycin D, Rifamycin and α-Amanitin are antibiotics that work by binding to molecules in
a cell. This inhibits protein synthesis.

The scientists who developed these antibiotics had to find out which types of cell were
affected and which molecule they were binding to.

The table shows the types of cell that these antibiotics affect and the molecule that they bind
to.

(i) Describe the structure of a DNA molecule.

(3)
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Edexcel (B) Biology A-level - DNA and Protein Synthesis

(ii) Explain the effect of Actinomycin D on protein synthesis.

(3)
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(iii) Explain the effect of Rifamycin and α-Amanitin on protein synthesis.

(3)
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(Total for question = 9 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q17.

Answer the questions with a cross in the boxes you think are correct . If you
change your mind about an answer, put a line through the box and then mark your
new answer with a cross .

Short sequences of nucleotides are being developed as potential drugs.

They act by binding to selected sites on DNA or RNA molecules and prevent the
synthesis of a specific protein associated with a disease.

Two types of drug to treat genetic disorders are:

• antisense drugs, which are RNA nucleotides that bind to mRNA

• triplex drugs, which are DNA nucleotides that bind to DNA forming a three-stranded
helix.

(i) The type of bonds that hold the two strands of a DNA molecule together in a double helix
are
(1)
A glycosidic bonds
B hydrogen bonds
C phosphodiester bonds
D peptide bonds

(ii) Antisense drugs inhibit protein synthesis by interfering with

(1)
A protein folding
B replication
C transcription
D translation

(iii) Triplex drugs inhibit protein synthesis by interfering with

(1)
A protein folding
B replication
C transcription
D translation

(Total for question = 3 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q18.

A student investigated the effect of three inhibitors on the growth of pollen tubes.
Pollen grains were incubated in a control medium.
In addition, some pollen grains were also incubated in three media, each containing one
inhibitor.
The inhibitors used were actinomycin D (act D), cycloheximide (CHX) and cytochalasin B
(CB).
The results of this investigation are shown in the graph.

The student made the following conclusions from these results:

Conclusion 1: all three inhibitors affected pollen tube growth

Conclusion 2: pollen grown in CB grew the slowest

Conclusion 3: the control result was the least repeatable

Conclusion 4: more pollen grains germinated in the control group

(i) Actinomycin D (act D) inhibits transcription.

Explain why pollen tubes could still grow in the presence of act D.
(2)
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Edexcel (B) Biology A-level - DNA and Protein Synthesis

(ii) Cycloheximide inhibits translation.

Describe the process of translation.
(4)
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(iii) Cytochalasin B prevents the addition of monomers in the synthesis of the protein actin.
Describe how monomers are bonded to a polypeptide chain during the synthesis of actin.
(2)
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(Total for question = 8 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q19.

Some antibiotics work by inhibiting the production of ribosomes in bacteria.

The structure of ribosomes in bacteria is similar to the structure of ribosomes in

mitochondria.

(i) Describe the structure of a ribosome.

(2)
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(ii) Describe the role of ribosomes in protein synthesis.

(2)
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(Total for question = 4 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q20.

Answer the questions with a cross in the boxes you think are correct . If you
change your mind about an answer, put a line through the box and then mark your
new answer with a cross .

The diagram represents part of protein synthesis.

(i) Name the stage of protein synthesis shown in the diagram.

(1)
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(ii) The stage shown in the diagram takes place in the

(1)
A cytoplasm
B Golgi apparatus
C nucleus
D smooth endoplasmic reticulum

(iii) The part labelled X on the diagram represents

(1)
A an ester bond
B a glycosidic bond
C a hydrogen bond
D a peptide bond

(iv) The part labelled Y on the diagram represents

(1)
A an anticodon
B a base pair
C a codon
D a DNA triplet

(v) Name the molecule represented by the part labelled Z on the diagram.
(1)
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(Total for question = 5 marks)
Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q21.

Which of the following statements is true for the total number of bases in a double-stranded
DNA molecule?
(1)

(Total for question = 1 mark)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q22.

Gibberellin stimulates cells in the aleurone layer of cereal grains, such as barley, to produce
the enzyme amylase.

The diagram shows a cell from the aleurone layer with some of the structures involved in the
production of amylase.

Gibberellin binds to a protein receptor in the cell surface membrane and this stimulates
transcription in the nucleus.

(i) Describe the process of transcription in the nucleus of this cell.

(2)
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Edexcel (B) Biology A-level - DNA and Protein Synthesis

(ii) Describe the processes occurring after transcription that result in the release
of amylase from the cell shown in the diagram.
(5)
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(Total for question = 7 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q23.

Answer the questions with a cross in the boxes you think are correct . If you
change your mind about an answer, put a line through the box and then mark your
new answer with a cross .

The diagram shows the ultrastructure of an animal cell.

(i) The structure labelled R on the diagram represents a

(1)
A chloroplast
B lysosome
C mitochondrion
D ribosome

(ii) The structure labelled Q on the diagram represents the

(1)
A cell surface membrane
B nuclear envelope
C rough endoplasmic reticulum
D smooth endoplasmic reticulum

(iii) Calculate the actual diameter of the structure labelled U.

(2)

Answer ...........................................................
Edexcel (B) Biology A-level - DNA and Protein Synthesis

(iv) Describe how the structures labelled P, S and T are involved in the production
and secretion of molecules from this cell.
(3)
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(Total for question = 7 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q24.

Ultraviolet (UV) radiation damages DNA and is used in microbiology to produce aseptic
conditions.

The UV radiation changes the bonding found in DNA.

This change in bonding creates thymine dimers in which covalent bonds form between
adjacent thymine nucleotides.

Thymine dimers bend the DNA backbone. This prevents replication of DNA.

The diagram shows the change in bonding caused by UV radiation.

Explain why preventing the replication of DNA produces aseptic conditions.

(2)
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(Total for question = 2 marks)

Edexcel (B) Biology A-level - DNA and Protein Synthesis

Mark Scheme
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Edexcel (B) Biology A-level - DNA and Protein Synthesis

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Edexcel (B) Biology A-level - DNA and Protein Synthesis

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Edexcel (B) Biology A-level - DNA and Protein Synthesis

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Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q23.
Edexcel (B) Biology A-level - DNA and Protein Synthesis

Q24.
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