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    Biotech Q3 WK 3-4

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    pia
    The document provides directions for students to read about and answer questions on Dolly the sheep, the first cloned mammal, who was created in 1996 at the Roslin Institute in Scotland by transferring the nucleus of a mammary gland cell from a Finn Dorset sheep into an egg cell from a Scottish Blackface sheep. It then explains the multi-step process of cloning a gene into a bacterial plasmid vector for propagation and identifies the key steps as isolation of DNA, insertion into the vector, transformation of bacterial cells, selection of transformed colonies, and identification of clones containing the gene of interest.

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    Biotech Q3 WK 3-4

    Uploaded by

    pia
    31 views2 pages
    The document provides directions for students to read about and answer questions on Dolly the sheep, the first cloned mammal, who was created in 1996 at the Roslin Institute in Scotland by transferring the nucleus of a mammary gland cell from a Finn Dorset sheep into an egg cell from a Scottish Blackface sheep. It then explains the multi-step process of cloning a gene into a bacterial plasmid vector for propagation and identifies the key steps as isolation of DNA, insertion into the vector, transformation of bacterial cells, selection of transformed colonies, and identification of clones containing the gene of interest.

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    Biotechnology modules

    Original Title

    BIOTECH Q3 WK 3-4

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    The document provides directions for students to read about and answer questions on Dolly the sheep, the first cloned mammal, who was created in 1996 at the Roslin Institute in Scotland by transferring the nucleus of a mammary gland cell from a Finn Dorset sheep into an egg cell from a Scottish Blackface sheep. It then explains the multi-step process of cloning a gene into a bacterial plasmid vector for propagation and identifies the key steps as isolation of DNA, insertion into the vector, transformation of bacterial cells, selection of transformed colonies, and identification of clones containing the gene of interest.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    31 views2 pages

    Biotech Q3 WK 3-4

    Uploaded by

    pia
    The document provides directions for students to read about and answer questions on Dolly the sheep, the first cloned mammal, who was created in 1996 at the Roslin Institute in Scotland by transferring the nucleus of a mammary gland cell from a Finn Dorset sheep into an egg cell from a Scottish Blackface sheep. It then explains the multi-step process of cloning a gene into a bacterial plasmid vector for propagation and identifies the key steps as isolation of DNA, insertion into the vector, transformation of bacterial cells, selection of transformed colonies, and identification of clones containing the gene of interest.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Republic of the Philippines

    Department of Education
    REGION III-CENTRAL LUZON
    SCHOOLS DIVISION OF TARLAC PROVINCE
    MALACAMPA NATIONAL HIGH SCHOOL-STE

    DIRECTIONS: Read and analyze the following concepts. Answer the activities on a
    separate sheet of paper.

    BIOTECHNOLOGY QUARTER 3 WEEK 3-4


    HOW GENETIC MATERIALS ARE MANIPULATED

    So now let’s learn what is genetic manipulation, it is the process of


    inducing changes in gene expression and expression of novel genes and has proven
    to be an indispensable tool in genetic research.
    According to Bhagvanji, Gohil Sanjay (2018) one of the applications of
    genetic manipulation is Gene cloning, defined as a mechanism by which each time
    the host cell reproduces; several copies of a particular gene are produced wherein it
    is possible to clone whole species. Gene Cloning is the insertion of a fragment of
    DNA carrying a gene into a cloning vector and subsequent propagation of
    recombinant DNA molecules into many copies is known as gene cloning.
    Nuclear cloning or Nuclear transfer- the introduction of the nucleus
    from a cell into an enucleated egg cell (an egg cell that has had its own nucleus
    removed). This can be accomplished through fusion of the cell to the egg or
    through the direct
    removal of the nucleus from the cell and the subsequent transplantation of that
    nucleus into the enucleated egg cell.
    Transgenic organism- Modern genetic technology can be used to modify
    the genomes of living organisms. This process is also known as “genetic
    engineering. “Genes of one species can be modified, or genes can be transplanted
    from one species to another. Genetic engineering is made possible by recombinant
    DNA technology. Organisms that have altered genomes are known as transgenic.
    Most transgenic organisms are generated in the laboratory for research purposes.
    For example, “knock-out” mice are transgenic mice that have a particular gene of
    interest disabled.

    The process of cloning a gene in a bacterial plasmid can be divided into five steps:
    1. Isolation of vector and gene-source DNA. The source DNA may come from
    human tissue cells. The source of the plasmid is typically E. coli. This plasmid white face was one of the first signs that she was a clone because if she was
    carries useful genes, such as ampR, conferring resistance to the antibiotic genetically related to her surrogate mother, she would have had a black face.
    ampicillin. In 2001, Dolly was diagnosed with arthritis after farm staff noticed her
    2. Insertion of DNA into the vector. By digesting both the plasmid and human walking stiffly. This was successfully treated with anti-inflammatory medication,
    DNA with the same restriction enzyme we can create thousands of human DNA although the cause of the arthritis was never discovered. Dolly continued to have a
    fragments,one fragment with the gene that we want, and with compatible sticky normal quality of life until February 2003, when she developed a cough. A CT scan
    ends on bacterial plasmids. After mixing, the human fragments and cut plasmids showed tumors growing in her lungs and the decision was made to euthanize Dolly
    form rather than risk her suffering. Dolly was put to sleep on 14th February 2003, at the
    complementary pairs that are then joined by DNA ligase. This creates a mixture of age of six.
    recombinant DNA molecules.
    3. Introduction of the cloning vector into cells. Bacterial cells take up the Name:
    recombinant plasmids by the transformation. This creates a diverse pool of Note: Use the remaining blank spaces as an answer sheet. You can use additional
    bacteria, sheet for your answers. Return this module.
    some bacteria that have taken up the desired recombinant plasmid DNA, other
    bacteria that have taken up other DNA, both recombinant and nonrecombinant. Guide questions:
    4. Cloning of cells (and foreign genes). We can plate out the transformed bacteria 1. Who is Dolly the Sheep and how was she created?
    on a solid nutrient medium containing ampicillin. Only bacteria that have the 2. Who made Dolly the Sheep?
    ampicillin-resistance plasmid will grow. 3. What was the first sign that showed Dolly was cloned?
    5. Identifying cell clones with the right gene. In the final step, we will sort
    through Assessment1.
    the thousands of bacterial colonies with foreign DNA to find those containing our 1. Why do you think producing Dolly is an important breakthrough in Science
    gene of interest. and Technology?
    2. If you were chosen to be a part of the team who created Dolly, what else would
    Activity 1 Dolly: The cloned sheep you consider so Dolly would be healthy as possible?
    Direction: Read the short article about Dolly the sheep and answer the following
    guide questions. Assessment2.
    Dolly was part of a series of experiments at The Roslin Institute that was 1. What are the two types of Cloning?
    trying to develop a better method for producing genetically modified livestock. If 2. What is Gene Cloning? How about Nuclear Cloning?
    successful, this would mean fewer animals would need to be used in future 3. What type of cloning did scientists use to create Dolly? Explain your answer?
    experiments. Scientists at Roslin also wanted to learn more about how cells change
    during development and whether a specialized cell, such as a skin or brain cell,
    could be used to make a whole new animal.
    These experiments were carried out at The Roslin Institute by a team led
    by
    Professor Sir Ian Wilmut. Because of the nature of the research, the team was made
    up of many different people, including scientists, embryologists, surgeons, vets and
    farm staff.
    Dolly was cloned from a cell taken from the mammary gland of a six-year-
    old Finn Dorset sheep and an egg cell taken from a Scottish Blackface sheep. She
    was born to her Scottish Blackface surrogate mother on 5th July 1996. Dolly’s

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