Phosphorus MD

PHOSPHORUS -UV
Phosphorus
Phosphomolybdate. UV
Quantitative determination of phosphorus SPIN640 APPLICATION

IVD
Store at 2-8ºC
PRINCIPLE OF THE METHOD
Direct method for determining inorganic phosphate. Inorganic phosphate
reacts in acid medium with ammonium molybdate to form a
phosphomolybdate complex with yellow colour. The intensity of the color
formed is proportional to the inorganic phosphorus concentration in the
sample1,2.
CLINICAL SIGNIFICANCE
Phosphorus is an essential mineral for tissue bone formation and is
required by every cell in the body for normal function. Approximately 85%
of the body phosphorus is found in bone and in teeth. Low levels of
phosphorus can be caused by hypervitaminosis D, primary
hyperparathyroidism, renal tubular disorders, antacids or malabsortion.
High levels of phosphorus can be caused by diet, bone metastases, liver
disease, alcohol ingestion, diarrhea and vomiting1,5,6.
Clinical diagnosis should not be made on a single test result; it should
integrate clinical and other laboratory data.
SPIN640Plus APPLICATION
REAGENTS
Ammonium molybdate 0,40 mM
R
Sulphuric acid (SO4H2) 210 mM
Molybdic
Detergents
PRECAUTIONS
H314-Causes severe skin burns and eye damage.
Follow the precautionary statements given in MSDS and label of the
product.
PREPARATION
The reagent is ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on the
The Calibration is stable until 36 days. After this period the Calibration must be
label when stored tightly closed at 2-8ºC, protected from light and
performed again in order to obtain good results.
contaminations prevented during their use. Do not use reagents over the
expiration date.
PERFORMANCE CHARACTERISTICS
Signs of reagent deterioration: Measuring range: From detection limit of 0,000 mg/dL to linearity limit of 35
- Presence of particles and turbidity. mg/dL.
- Blank absorbance (A) at 340 nm  0,54. If the results obtained were greater than linearity limit, dilute the sample 1/2 with
NaCl 9 g/L and multiply the result by 2.
ADDITIONAL EQUIPMENT Precision:
- SPIN640 / SPIN640Plus Autoanalyzer. Intra-assay (n=20) Inter-assay (n=20)
- General laboratory equipment (Note 1). Mean (mg/dL) 4,09 7,12 4,11 7,09
SAMPLES SD 0,03 0,046 0,09 0,06
- Serum or plasma1,5: CV (%) 0,62 0,80 2,15 0,80
Free of hemolysis. Serum or plasma should be removed from the clot Sensitivity: 1 mg/dL = 0,0798 A.
as quickly as possible to avoid elevation of serum phosphorus from Accuracy: Results obtained using SPINREACT reagents (y) did not show
hydrolysis or leakage of phosphate present in erythrocytes. Stability: 7 systematic differences when compared with other commercial reagents (x).
days at 2-8ºC. The results obtained using 50 samples were the following:
- Urine1,2 (24 h): Correlation coefficient (r) 2: 0,8577.
Collect the specimen into a bottle containing 10 mL of 10% v/v Regression equation: y= 0,724x + 0,837.
hydrochloric acid (HCl) to avoid phosphate precipitations. Adjust to pH The results of the performance characteristics depend on the analyzer used.
2. Dilute the sample 1/10 with distilled water. Mix. Multiply the result by
10 (dilution factor). Stability: 10 days at 2-8ºC. NOTES
1. Most of the detergents and water softening products used in the laboratories
1
REFERENCE VALUES contain chelating agents and phosphates. It is recommended to rinse
Serum or plasma: glassware in diluted nitric acid and water before using.
Children 4,0 – 7,0 mg/dL  1,29 – 2.26 mmol/L 2. Calibration with the aqueous standard may cause a systematic error in
Adults 2,5 – 5,0 mg/dL  0.80 – 1.61 mmol/L automatic procedures. In these cases, it is recommended to use a serum
Urine: Calibrator.
Adults 0.4 – 1.3 g /24 h 3. Use clean disposable pipette tips for its dispensation.
These values are for orientation purpose; each laboratory should
establish its own reference range. BIBLIOGRAPHY
1. Farrell E C. Phosphorus. Kaplan A et al. Clin Chem The C.V. Mosby Co. St
QUALITY CONTROL Louis. Toronto. Princeton 1984; 1072-1074 and 418.
Control sera and calibrators are recommended to monitor the 2. Daly J A. et al. Clin Chem 1972; 18 (3): 263-265.
performance of assay procedures: SPINTROL H Calibrator, SPINTROL 3. Young DS. Effects of drugs on Clinical Lab. Tests, 4th ed AACC Press, 1995.
H Normal and Pathologic (Ref. 1002011, 1002120 and 1002210). 4. Young DS. Effects of disease on Clinical Lab. Tests, 4th ed. AACC 2001.
If control values are found outside the defined range, check the 5. Burtis A. et al. Tietz Textbook of Clinical Chemistry, 3rd ed. AACC 1999.
instrument, reagents and technique for problems. 6. Tietz N W et al. Clinical Guide to Laboratory Tests, 3rd ed. AACC 1995.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances. PACKAGING
BARCODED REAGENTS LOAD MUST BE PRECEDED OF A Ref: MD1001155 Cont. R: 6 x 40 mL
SPINREACT “DATABASE” COPY INTO THE ANALYZER
SOFTWARE. IT IS AVAILABLE UNDER REQUEST TO SPINREACT.
MDBSIS15-I 27/04/17 SPINREACT,S.A./S.A.U. Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN
Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail: [email protected]

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PHOSPHORUS -UV

Quantitative determination of phosphorus SPIN640 APPLICATION

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