TS Module - 2 oO PR DNA by Sriation ° CE Meer ea Be es conovisicl » ieee crm eet hy “ee 13, mer 9- men v v 7 3 mab of 8 bf Gr emecpccens gro ntP - Rich an AY + High affenity fo~ Drah Pusha = Lalli Dnah aceembly ragion + Celtel DNA rerwsinclung ehorerile 2 every cre, re = him , Toraron oufrrcarlorny Gece tow © Dnan brch to 9 min ryprale along with ATP v Sreliuco Sopologreal diem , risulo in mrulteng (gring p) ¥ AT such» 13 Tun Otquuncio + Draw proten now Ginde to (13 mer suguumcio - DnaB & leacect ole te Dna by Drac protein (it har a tromeitory not) « Drak hac tulicace acliuil, » it Cuche tn bax b/a Be iE ng weg 0 ho + Thin smcluas topalagical oan euprcoitiel DNA. The lun io muuch by Dna Yynoe by ugaitrite oupencoiling + eingle shramduel Buncling ($88) Protein Crick 20 apaticl strand, a oie obuuctare du to Ge Yat en fret eh 4 Ylk> Elongation . th formation ryplication , tach OWA talicase ee the pl (en iff On a gm) thet oy lhe’ dod Ht Facial ce 7” Gs Beblee 810 5 sacle ee aad RNA 4g ment ¥ nes atrond a welch ao DNA prolymucace com add nucleoli to Seay oteanck ancl canal oyrthucixe ne franck «© Dna Polymuox Ik 70 bnc to rn ancl ackely Tuclotice Lo Lhin Youur in $' lo 3’ chiruclion Ao th dongation Proctcle . the Cubble ortind Cicticusnel, cts) beim vrclucicl 4 rebierueck 2 ally topological %y Own gy oly muricalion Ye marilain §' to 8' polanil, wy ONA poy DNA & ayristoircl 4r oppocits oteance eitclion on the ito g ee Hreadtiig obeand —> came cueielion ao movwernent (ot 2 rej eliciation Be an regtitio avrgle foocmn Hagging Seance > Gif. chnicltin (ian areca > chocontinuous gy7diinin of DNA > Zaki ohand au gyda zk She a Hin jor wile a contiriou Dwh obvanc tu adit, of 2A frogymnce | anck DNA Cha zaki ) wa polymnoe D hare ymumaa T and amb pwmonin RNA biirur om 2) DNA >» proadue ‘dhone one gyrtheire QnA to rplas it . i ] Es ssnsesetetei seussBN) RNA fume Mmowiel > Tee 7 =~ A rich remain Yo 2 fragment ~~ > DNA Liga tm joire Bo mich nel toner BOM THE FORKS EncouNTER Non - PERMISSIVE STTE OF TER SEQUENCES AND REPLICATION STOPS. Oe dovmialion seplucalion 2 done Cy TER equines and Tus protun “an combumalion dau 10 TER Agumen 4) E coli. ach JER oquner tas e oo ae rocud) anc a non- hatteEndl. - Replication B in Prokanyols Shon | replication aleecricila® SONA) 2) carne aat circular ctowightn” DNA remain ereoavericlicl Buch srtodockih wbucturto au callieh Codinaruo Tho to obruérely 2 problem ao tach clasughtn edt tao tabu only one DNA molecule Bind to tu ds DNA and cut tu POq Gok eee DNA anc alla. DNA to “unbound e oe - Ls tm eo - oe © — Lahayotc ONA plication * In sukaryotn tu ONA syplicalion cccuro én S- proce of Pu all Sele Ge Checmemerrlale omer. sbliction, Ona ryblication & of bi- scluctional 4 Bre ee 50 - 300 Kb . The human gunoms hao about 30/000 wuyin 9 ryblication . > _Gniliation + Gagen of replication bo AT~ 70h ragtom Ly Gr Yeast - ARS. C Adtonomously Mypleating Sure ) Proturo eb 1) ORe - Origin Rplicain Lomplen ty Brice to ORT ty ido ATP ad's 5 Reouilo al otw Drache fon uriletion . i) CdO-6 Preatum ( Kile Durucion yeh - 6 Protun) _ L Ragusac fon alice toacrig -~ ~~ ~~ _ ~~ ~ ii) CD1-1 (cnc 1 Dyfnetint Sremocript 1) Beohain Ly Ae. mcm Loaclmg Ly Fubonachs wath MEM andl induct topological aovoctalion of mcm a7 wilh DNA yy etcH 2-1 (_Mimichwomeomal masnJenane)- Hlidaoe actesily ) Nalijnatom orothalmolaney Cids fae ret a 9) Dk / DDk ( Gyetn cipndird. Kinooe /- Dey 4 - Diforetind. heres) 4 Regulate initiation ppotum Gy Pracpharyellion vi) CDC-_43 (GU Derwovon eye 45) Protin L, Srtorade with McH-7 ancl Polymuman 2X b Aedutatn fulicacs atl of MOM preare - vi) GINS I as Sritiedion cape by Soume CMG acoimbly vii) REA Arolain ( Mppcaiton Protum - A) b Burserte ssammeailting of DNA obvancl 5 588 protun tke i) REC ( Rpuication Sactan ©) Ly DNA clamp toad Ly head PCNA x) sPONA Ly Hole DNA sma sig the monmer BDNA ORC pprotun Grinch to ORT ih iS L protum Lrough AIP ae actinucly CDC-6 anc C2T-\ au toaducl Shy men meow MCM 2-7 cOc-6 Ga- = Be s cael QUllRle le Pete Dhol op Phas Tree IE TET Ment Ctdscaoe) caT-! L PRE - REPLICATIVE COMPIEA ) Dao net chow bulcon acduut, (tll tracti ) . CDk Phopronplatio cDc-6 ¥g ee DDk —phaxphowlato McM S- phase COI-' 4 webnhibiticl ey GEMINIM protan ORC 2» abo phhobharylaticl 0 if claw not rmcrut ony rilialton poe ° Dignadation 4 oDe-6 Srbubilion GEMINIM 0 McM MOM Ring (muctustich 4 SD-2 4 SUD = 7 Cony) Bln raceutrrment eDe 45 amd GINS ® De 4s ‘ © 5 3° ae Phocphonyalid GINS. Men CMG Bosrnble ° CMG mows acm tu DIVA and mlb th DNA: - CEG oor yee) suouik MCM -10 anc CTF 4 protein that Yn tn resi RPA amd Polymuvan anc PrimesPe on apy tow + Puma adlelo re on tadirag obearch cortirisowely os re 7 Polymesooe ot worke on thas ppomve one to smilie the oy7thio PCNA clamp attach to te lomo 5 - Colyruna now acgocicite wth thes PONA clompo fon lee % DNA obwancle & Elongaiion scones ohand DONA gyrthia ‘0 ordinate ° Portrayed One yr io = decent ee . Dine na probtem in aprile of tegpray ome CECE ECE CEE CE ECC CEES oe, Alo th CMG cmmbly mowe» on Uadlieg Shondl thn 2" onc pre adic Wa om te Laggrig sivancl Thun 2 5! enel.. hin Polymunane S sncowrtoe fourm on Aatgeng omanek» f chiblace ta _pprrinn ah fiat rel Bis ale on Oho aol Orumno ae remorucl by RNAae H - enzyme Polymirace 5 ayrtiaize ppwinin ope 2 Oka - rahi sugmamio ar thin wall by DNA 149-008 Most the tin, replication 0 Iommncitid whim thio replicator : rar) © SCF 63 Ubiquitin digam compton ubiquiiale CM - 7 ° Mem gt ppolpsbsgpitunallich on te Yyoore mols ‘4 Ubiguiamalick MCM- 7 to Yucagmizid by p97 ( protam rumoclulie,) Which cauto divacarmbly of CMG, compli . Tho, DVR syplication bo teminalict ky Delevan Replicas On tu tagging olonde chim primmuno are rumenich oncl vyplaad by cory ructiolidia » emmy OMA ot nveplicalicl end gy Yumatino Z * oy Sx eee rs Prarie orn rumored amok Poly movers 2 fil ie — a . PPPPPPTFIPPPPIPIVPVP PEP POTITAG Pe ee eeeOCC CeCe eee eee EEE th 5 pa 4 T algeer 4 tad. y. Aiaagino re es fen--4 - but, ve tame corre im I ft Se Rant in shovaneig of O00 Sf MY FX vuoulling ” 29°Y ayn Selomtons anya! fos tte arm RA a oral ac sachiily 00 wl gelomnina 703. wy Seg tema eS ee Oe ae 7 ae anced aa (A (Ss aad Shlomuaa imryme io rmorielENZYMES / PROTEIN Brokaryoto Drah Beohim - i ies Sritiaton Enry re "Sritintn aes 2 AGT ah ORI-C SONA oly rmetaate se Purmace Pfpticalron ard rupaur amt nfl ; (teach Drab DnaA) a g Dna Protin Srclucio - ue seluriin clea While Dubro elon wriiincliol by Aulicooe DNA higac = Lget. . DNA Gyraoe CB cubwnit sa a ond ig fo Bemorwuo sprimrun posure anmialing TUs/ TER Prclan : Tormunectlion RNAge SSBo @ it e Prarie cactosity ce Hadung A ae aphiaio ‘_ *