NAME 

: 

CLASS : 
Gene Cloning
DATE  : 
20 Questions

1. Restriction enzymes are analogous to what common school supply?

A scissors B glue

C highlighter D paper

2. Restriction Enzymes can cut at any sequence of DNA

A False B True

3. The site where restriction enzymes cut are a called:

A cut sites B cleave sites

C recognition sites D restriction sites

4. Where are restriction enzymes naturally found?

they are not natural - all restriction enzymes are made

C in humans D in bacteria

5. What is a plasmid?

A an accessory piece of DNA found in bacteria B viral DNA

C a material that cuts DNA D a section of the human genome

6. If a linear piece of DNA contains 3 recognition sites for a restriction enzyme. How many pieces will be produced when
the restriction enzyme cuts the DNA?

A 2 B 5

C 4 D 6

E 3

7. Sticky ends are produced when the restriction enzyme cuts in a:

A staggered manner B straight through the DNA

8. A plasmid is larger than a bacterial genome

A True B False

9. Plasmids often have genes that give the bacterial a survival advantage

A True B False
10. The multiple cloning site in the plasmid only allows one restriction enzyme to be able to cut open the plasmid

A True B False

11. What enzyme binds to the promotor of the plasmid to transcribe the gene inserted into the plasmid?

A helicase B RNA polymerase

C DNA polymerase D ligase

12. How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to
insert into a plasmid?

A 3 - through the gene and on either side of the gene B 1 - through the gene

C 4 - twice on each side of the gene D 2 - on either side of the gene

13. What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate
backbone?

A ligase B DNA polymerase

C helicase D RNA polymerase

14. Biological cloning is frequently used to make which of the following:

A genetically modified oil eating microbes B Human insulin

C all of the options D Human growth hormone

E pest resistant crops

15. Chemical cloning uses a machine called a thermocycler to clone the gene

A False B True

16. Each cycle of the thermocycler doubles the number of DNA copies. How many cycles of the thermocycler are needed
to make 16 copies of DNA?

A 3 B 6

C 4 D 5

E 2

17. Why are primers needed for the PCR process?

they attach to sites on the DNA on either side of the

C they are used to build more copies of the DNA D they are what change temperature to copy the DNA

18. Why is DNA polymerase needed in the PCR process?

A they are the building blocks to make more DNA B it attaches to either end of the gene to be copied

it is what makes a complementary copy of the template

they are the machine that changes temperature to they attach to either end of the DNA that needs to be
A B
make DNA copies copied

they are the building blocks used to build the copies of they are the template that is used to make copies of
C D
DNA the DNA

20. Why is the first heating up stage needed in the PCR cycle?

it allows the DNA polymerase to attach to the DNA to it unwinds the double stranded DNA so both strands
A B
begin copying it can be used as a template to make more DNA

C it attaches the nucleotides to make DNA D it allows the primers to attach the DNA
Answer Key

1.a 2.a 3.c 4.d

5.a 6.c 7.a 8.b

9.a 10.b 11.b 12.d

13.a 14.c 15.b 16.c

17.b 18.d 19.c 20.b