Manual INCUBADORA THERMO 3110 PDF

User Manual
Model 3100 Series

Forma Series II Water Jacket CO2 Incubator
Operating and Maintenance Manual 7053110 Rev. 2
Visit us online to register your warranty

www.thermoscientific.com/labwarranty
Downloaded from www.Manualslib.com manuals search engine

Preface
Table 1. Single Chamber Models
Model CO2 Sensor* O2 Voltage ** Temp Max
3110 T/C No 115 55C
3111 T/C No 230 55C
3120 IR No 115 50C
3121 IR No 230 50C
3130 T/C Yes 115 45C
3131 T/C Yes 230 45C
3140 IR Yes 115 45C
3141 IR Yes 230 45C
*T/C is a thermal conductivity sensor.

IR is an infrared sensor.
**All units are 50/60 Hz.
MANUAL NUMBER 7053110

2 40087/IN-4743 7/28/16 Changed label on remote alarm artwork to note - pg 6-1 ccs
1 40421 11/2/15 Added risk assessment information ccs
0 27988/IN-4478 4/7/14 Release 7 - Original ccs
REV ECR/ECN DATE DESCRIPTION By
Thermo Scientific Water Jacketed CO2 Incubator i

Preface
CAUTION
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
Important Read this operating manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Warning All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Intended Use:
The Thermo Scientific Water-jacketed CO2 incubators listed in this manual are intended to provide an
environment with controlled temperature and Carbon Dioxide (CO2) as well as elevated humidity (RH) for the
growth of microbiological samples or mammalian cells for research (FDA Class 1), and IVF and other regulated
cell therapy applications (FDA Class 2). Certain models also control Oxygen (O2) at suppressed levels via the
injection of Nitrogen (N2).
For FDA Class 1 applications, the intended end user is a laboratory / medical professional trained in basic safety
and cell culture practices. For FDA Class 2 applications, the intended user is a trained Laboratory Technician
whose qualifications include a bachelor’s or master’ss degree in chemical, physical, biological, medical
technology, clinical or reproductive laboratory science from an accredited institution.
These incubators are designed to be used indoors only and operate 24 hours per day - 7 days per week, but
should be shut down regularly to be disinfected to prevent contamination, maintained per the instructions for
use, and calibrated to retain optimum system performance.
Non- intended use:
These incubators are not intended for use by the general public.
These units are not intended for use within the patient environment. These units are not to be used outdoors.
These incubators are not designed to provide O2 levels greater than 20% and as such should never be connected
to O2 as it is highly flammable in elevated concentrations. These units are not designed to be used with Liquid
CO2 or Liquid N2. These units should not be used with any flammable or toxic materials.
©2014 Thermo Fisher Scientific. All rights reserved.
ii Water Jacketed CO2 Incubator Thermo Scientific

Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Asphyxiation Hazard Warning. High concentrations of CO2 and N2 can displace oxygen and cause
asphyxiation!
Lifting Hazard Warning. The incubator weighs more than 200lbs (91kgs). Take adequate safety
measures when moving this device.
4-Person Lift. The incubator weighs more than 200lbs (91kgs). It is recommended that four persons
lift this device together to move it.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [email protected].
4 Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4 Always dissipate extreme cold or heat and wear protective clothing.
4 Always follow good hygiene practices.
4 Each individual is responsible for his or her own safety.
Thermo Scientific Water Jacketed CO2 Incubator iii

Preface
Do You Need Information or Assistance on

Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermoscientific.com Internet Worldwide Web Home Page
[email protected] Tech Support Email Address
www.unitylabservices.com Certified Service Web Page
Our Sales Support staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.
iv Water Jacketed CO2 Incubator Thermo Scientific

Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
• Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.
• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
• Repairs to parts or systems resulting from unauthorized unit modifications.
• Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO2 sensors, blower motors, water jackets, etc.
Thermo Scientific Water Jacketed CO2 Incubator v

Table of Contents
Section 1 Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Name and Description of Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Installing the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Installing Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Installing HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Installing Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Installing Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Connecting Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . .1-10
Filling Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Filling Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Connecting CO2 or N2 (O2 System) Gas Supply . . . . . . . . . . . . .1-12
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-14
Setting the Operating Temperature . . . . . . . . . . . . . . . . . . . . . . .1-14
Setting the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . .1-15
Setting CO2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-15
Setting O2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-16
Section 2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Temperature Stabilization Periods . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Calibrating T/C CO2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
T/C CO2 Sensor Stabilization Periods . . . . . . . . . . . . . . . . . . . . . .2-3
Calibrating Infra-Red CO2 System . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
IR CO2 Sensor Stabilization Times . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Calibrating the O2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
O2 Calibration at 20.7% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
O2 Offset Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Calibrating Relative Humidity (RH) . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Relative Humidity Stabilization Times . . . . . . . . . . . . . . . . . . . . . .2-6
Thermo Scientific Water Jacketed CO2 Incubator vi

Table of Contents
Section 3 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Turning the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . .3-1
New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Setting the REPLACE HEPA Filter Reminder . . . . . . . . . . . . . . . . .3-2
Setting an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Setting Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Setting High Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Enabling Temp Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . .3-4
Setting Low CO2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Setting High CO2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Enabling CO2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-5
Setting New Zero # for T/C CO2 Sensors . . . . . . . . . . . . . . . . . . . . .3-5
Setting New Span # for T/C CO2 Sensors . . . . . . . . . . . . . . . . . . . . .3-6
Setting a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Enabling RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-7
Setting a Low O2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7
Setting a High O2 Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-8
Enabling O2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-8
Enabling Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . .3-9
Enabling CO2/O2 to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . . . .3-9
Selecting a Primary Tank (Gas Guard) . . . . . . . . . . . . . . . . . . . . . .3-10
Disabling the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . .3-10
Setting a RS485 Address (1535 Only) . . . . . . . . . . . . . . . . . . . . . . .3-10
Section 4 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Inner Door Open Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Section 5 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning the Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Cleaning Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Reversing the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Cleaning Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Replacing Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Replacing Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Replacing Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Draining Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Adding /Replenishing Rust Inhibitor . . . . . . . . . . . . . . . . . . . . . . . .5-11
Replacing O2 Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-12
vii Water Jacketed CO2 Incubator Thermo Scientific

Table of Contents
Section 6 Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Connecting the RS485 Interface (190523) . . . . . . . . . . . . . . . . . . . .6-2
Connecting Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Connecting CO2 Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Gas Guard for CO2 or N2 (190640/190642) . . . . . . . . . . . . . . . . . . .6-5
Connecting N2 Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6
De-activating Built-in Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6
Operation of CO2 or N2 Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . .6-6
Humidity Readout (190643) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-7
Cooling Coil . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-8
Installing Cooling Coil Incubator . . . . . . . . . . . . . . . . . . . . . . . . .6-10
Section 7 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Section 8 Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

All Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Spare Parts - 115V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Spare Parts - 230V . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Spare Parts - IR or O2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Spare Parts - Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Spare Parts - Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Field Installation Kits for qualified service personnel . . . . . . . . . . .8-3
Spare Parts - Data Output Options . . . . . . . . . . . . . . . . . . . . . . . .8-3
Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-4
Section 9 Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Section 10 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Section 11 Interior Door Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1
Thermo Scientific Water Jacketed CO2 Incubator viii

Section 1 Installation and Start-Up
Component Name and
Description
Figure 1-1. Water Jacket Incubator
• Outer Door - Reversible to opposite swing (Section 5).
• Heated Inner Door - Keeps chamber interior dry. Reversible to

opposite swing (Section 5).
• Chamber Gas Sample Port - Used for sampling chamber CO2 and
optional O2 content, for calibration (Section 2).
• Main Power Switch
• Control Panel - Keypad, Displays and Indicators (Figure 1-2).
• Fill Port - Used for filling the water jacket.
• Water Jacket Vent - Allows air to escape from the water jacket during
filling and normal expansion and contraction when the incubator heats
or cools.
• Leveling Legs - Used to level the unit.
• Water Jacket Drain – Use hose barb insert included.
Note The incubators are stackable. Instructions follow in this section. s
Thermo Scientific Water Jacketed CO2 Incubator 1-1

Section 1
Installation and Start-Up
Control Panel Keys, Message

Center Heat
Displays & Indicators Alarm Mode Model
Select
Up and
Indicator
Temp
Indicator Select Down
Silence Switch Indicators Arrows Enter Display
36.9
Run Set Cal Config Heat Temp
Silence Mode CLASS 100 Enter
4.9
Inject % CO2
Scroll for Parameters
Tank Low
Gas Guard Scroll for Inject %CO2/O2

Tank Low Indicator Parameters Keys Indicator Display
Figure 1-2. Control Panel
• Alarm Indicator - Pulses on/off during an alarm condition in the

cabinet.
• Silence - Silences the audible alarm.
• Tank Low Indicator - Used with Gas Guard, lights when gas tank is
low, if applicable.
• Mode Select Switch - Used to select Run, Setpoints, Calibration and
System Configuration Modes.
• Message Center - Displays system status.
• Mode Select Indicators - Run, Set, Cal, and Config Menus
• Up and Down Arrows - Increases or decreases number parameter
values, toggles choice parameter values.
• Enter - Accepts changes to calibration settings
• Heat Indicator - Lights when power is applied to heaters.
• Temp Display - Can be programmed to display temperature
continuously, RH continuously (with RH option), or toggle between
temperature and humidity (with RH option). See Configuration
Section.
• Scroll for Parameters Keys - Scrolls through parameters of selected
mode.
• Inject Indicator - Lights when gas is injected into the chamber. If the
%CO2/O2 display is continuously displaying CO2, the light indicates
CO2 injection only. If the %CO2/O2 display is continuously displaying
O2, the light indicates N2 injection. If the %CO2/O2 is toggling, either
a CO2 or N2 injection causes the indicator to light.
• %CO2/O2 Display - Can be programmed to display CO2 continuously
or O2 continuously (on units equipped with O2), or toggle between
CO2 and O2 (on units equipped with O2) See Configuration Section.
1-2 Water Jacketed CO2 Incubator Thermo Scientific

Section 1
Control Panel The Model 3100 Series water jacket incubator has four basic modes, which
allow incubator setup. The modes are as follows: Run, Setpoints,
Operation Calibration and System Configuration.
• Run is the default mode that the incubator will normally be in during
operation.
• Set is used to enter system setpoints for incubator operation.
• Cal is used to calibrate various system parameters to the customer’s

satisfaction.
• Config is the system configuration mode that allows for custom setup
of various options.
Scroll for Parameters Arrow Keys: Steps the operator through the parame-
ters of SET, CAL and CONFIG Modes. The right arrow goes to the
next parameter, the left arrow returns to the previous parameter.
Up Arrow: Increases or toggles the parameter value that has been selected
in the SET, CAL, and CONFIG Modes.
Enter: Must press Enter key to save to memory all changed values.
Down Arrow: Decreases or toggles the parameter values that have been
selected in the SET, CAL and CONFIG Modes.
Silence Key: Press to silence the audible alarm. See Section 4 for alarm
ringback times.
Message Center: Displays the system status (Mode) at all times. Displays
CLASS 100 or SYSTEM OK during normal operation, or alarm mes-
sages if the system detects an alarm condition. See Section 4, Alarms.
The display message CLASS 100 is a timing mechanism indicating that,
under normal operating conditions with the HEPA filter installed, the
air inside the chamber meets the Class 100 air cleanliness standard for
particulates of 0.5 micron size or larger per cubic foot of air.
Upper and Lower Displays: These 7-segment displays vary depending
upon the options present and the configuration chosen. The upper dis-
play can display temp or RH, or toggle between them. The bottom dis-
play can display CO2 or O2, or toggle between them. See Section 3,
Configuration.

Section 1
RUN SETPOINT CALIBRATION CONFIGURATION

Default Mode Temperature Temp Offset Audible
Overtemp CO2 Cal New HEPA timer
CO2 IR CO2 Span Replace HEPA reminder
O2 O2 Cal at 20.7% Access Code
O2 Offset Temp Lo Alarm
RH Offset Temp Hi Alarm
Temp Relay
CO2 Lo Alarm
CO2 Hi Alarm
CO2 Relay
T/C CO2 Z #
T/C CO2 S #
RH Lo Alarm
RH Relay
O2 Lo Alarm
O2 Hi Alarm
O2 Relay
Display 1 Setup
Display 2 Setup
Gas Guard Setup
RS485 Address
Base Unit Displays
Option Displays
Installing the Unit 1. Locate the unit on a firm, level surface capable of supporting the unit’s
operational weight of 365 lbs. (166kg) plus the estimated total sample
and user equipment weight. The overall space should be well ventilated
and large enough that the concentration of carbon dioxide (CO2)
and/or nitrogen (N2) gases cannot accumulate, thus displacing the
oxygen (O2) to dangerous levels in the room. Read Connecting CO2
or N2 (O2 System) Gas Supply below carefully.
Note If two incubators are to be stacked, then the surface must be capable
of twice the weight estimated above.
2. Locate the incubator away from exterior doors and windows as changes
in outdoor temperatures and contact with direct sunlight can affect the
anti-condensation functions of the unit.
3. Position the door opening away from forced air heating and cooling
ducts as these can carry dust, dirt, and other contaminants into the
incubator chamber, as well as negatively affect the door opening
recovery time.

Section 1
Installing the Unit 4. Maintain a minimum six inch clearance behind the unit for electrical
and gas hook-up. In addition, a minimum three inch ventilation
(continued) clearance is needed on each side.
5. It is recommended that the unit be placed on a manufacturer approved

stand or roller base, not directly on the floor, as the air movement
created each time the door is opened will pull dust, dirt, and other
contaminants into the incubator chamber.
Warning This incubator weighs 265 lbs (120kg) before filling the water
jacket. If the unit has been in service, disconnect the power cord from
both the unit and the power source, turn off all gas regulators, and
disconnect all tubing and any other connections from the rear of the
electronics drawer before completely draining the water jacket. Do not lift
the incubator by the outer door as this could damage the hinges and/or the
outer door may come loose. Note that the outer door can be removed to
reduce weight and make it easier to lift the unit by its corners. Have
sufficient personnel available to lift the unit into place using proper lifting
techniques according to local EHS policies. Lift from both sides of the
unit (left and right, not front and back) holding it from the bottom. Do
not push or pull the incubator along the floor as this could damage the
leveling feet.
Stacking the Incubators Note Stacking brackets (shown at right) and

mounting hardware are included with each
Figure 1-3. Stacking brackets
unit. s
1. Designate one incubator to be the top unit and the other as the
bottom unit. Remove the base cover plate from the top unit using the
finger holes in the base or using a slotted screwdriver (Figure 1-4).
2. Note the two slots in the base of the incubator which accommodate
the stacking bolts. Refer to Figure 1-5.
Figure 1-4. Remove Base Cover Plate

Figure 1-5. Stacking Bolt Slots

Section 1
Stacking the Incubators 3. Remove the two plastic plugs

(continued) from the bolt holes in the exterior
top of the bottom unit. Install the
½” long 5/16-18 stacking bolts
and washers (Figure 1-6) into the
bolt holes - do not tighten the
bolts at this time.
Figure 1-6. Bolt Holes
4. Unscrew to remove the leveling
feet from the top unit. Lift it
onto the bottom unit, off-
setting the base of the top
unit approximately 2-3
inches behind the stacking (2) 5/16” bolts and
flat washer, ½" (13mm)
bolts and washers. socket wrench required
5. Align the sides of the top
unit and bottom unit. Slide
the top unit forward until
the slots in the base of the
top unit align with the
5/16”-18 stacking bolts in Figure 1-7. Align Slots and Bolts
the exterior top of the
bottom unit (Figure 1-7).
6. Remove and retain the two screws from the back of the control panel
on the bottom unit as identified in Figure 1-8.
Figure 1-8. Two Screws From Control Panel Back
7. Secure the stacking brackets to the back of the bottom unit, using the
retained screws (Figure 1-8). Be sure to align the brackets with the
mounting holes on the back of the upper incubator. Secure the
brackets to the upper unit with the ¼-20 bolts provided in the
stacking kit. A 7/16” wrench or socket is required for the bolts.

Section 1
Stacking the Incubators 8. From the unit front, secure the base of the top unit to the exterior top
(continued) of the bottom unit by tightening the 5/16-18 stacking bolts using a ½”
(13mm) wrench or suitable tool. Refer to Figure 1-7.
9. Replace the base cover on the top unit.
Preliminary Cleaning 1. Remove the protective plastic coating on the shelf supports, duct
sheets, and air duct, if present.
Caution Before using any cleaning or decontamination method except

those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment. s
2. Using a suitable laboratory disinfectant, such as quaternary

ammonium, thoroughly clean all interior surfaces to remove any
residues which may remain from production of the incubator. Refer to
Section 5.
Installing Access Port Locate the opening in the top left

Filter corner of the interior chamber.
Remove the tape from the
opening on the outside of the
unit. Locate the stopper with filter
in the hardware bag. Install in the
opening inside the chamber
(Figure 1-9).
Figure 1-9. Filter and Sensor Locations
Installing Air Sample 1. Remove the filter from the shipping bag.
Filter
2. Separate one section of the tubing from the filter. Install this section to
the fitting on the blower plate.
3. After installing the top duct, connect the filter assembly to the tubing
coming through the top duct.
4. Insert the free end of the air sample filter tubing into the larger hole in
the back of the blower scroll. See Figure 1-9 for completed
configuration.

Section 1
Installing HEPA Filter Note There are standard and Volatile Organic Chemical (VOC) HEPA
filters available. Be certain to install the correct filter for the given
application. See Section 8.
Caution Handle the HEPA filter very carefully as the filter media can be
easily damaged. Do not touch the filter media during installation.
1. Remove the filter from the shipping box.
2. Remove the plastic coating from the filter and inspect the filter for any
visible signs of damage.
3. Install the filter as shown in Figure 1-9.
4. The default HEPA filter replacement reminder was set at the factory
for 6 months. See Section 3 to change the timer value.
Caution To avoid damage to the incubator, do not operate the unit

without the HEPA filter in place. If higher RH is needed and CLASS 100
air quality conditions are not required, use the restrictor plate instead of
the HEPA filter in order to maintain proper air flow. s
Installing Shelves 1. Install the side ducts with the tabs facing
into the center of the chamber with Side toward side duct
their slots up. There are no right side or Shelf Channels
left side ducts, simply rotate one of
Side toward shelf
them to fit the opposite side. Tilt the
side ducts as they are placed in the
chamber so the tops fit into the top air
duct, then guide them into the vertical
position. Figure 1-10 shows the duct as
it would be oriented for the right side of
Side Duct
the chamber.
Figure 1-10. Shelf Channels
2. Referring to Figure 1-10, note that there and Side Duct
is no difference between left and right
side shelf channels.

Section 1
Installing Shelves 3. Install the shelf channels by placing the channel’s rear slot over the
(continued) appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-11.
4. Figure 1-12 shows one of the channels installed on the right side duct.
Note that if a shelf is installed in any of the bottom 3 rows of side duct
tabs that it will need to be removed to take out the humidity pan to
clean and add water.
Shelf channel Side duct Shelf channel

rear slot tab front slot
Side duct
tab
Figure 1-11. Slot and Tab Figure 1-12. Channel Installed
5. The shelf can be installed with the sides up forming a tray that can be
easily removed with all of its samples, or with the sides down allowing
the samples to be carefully slid off individually. The shelf can be
partially pulled out to access samples not in the front, but be careful to
not pull the shelf out too far as there are no stops to prevent the shelf
from falling out.
Leveling the Unit Check the unit for being level by placing a bubble-style level on one of the
shelves. Turn hex nut on leveler counterclockwise to lengthen the leg, or
clockwise to shorten it. Level the unit front-to-back and left-to-right.
Connecting Unit to For electrical specifications, see the serial tag on the side of the unit,
Electrical Power Section 7 Specifications, or refer to the electrical schematics in Section 9
of this manual.
Warning Connect the incubator to a grounded dedicated circuit only.

The power cord connector is the mains disconnect device for the
incubator. Position the unit to allow unobstructed access to the power
cord so that it can be easily disconnected in case of an emergency. s
Plug the provided power cord into the power inlet connector (Figure 1-13)
and into the grounded dedicated circuit.

Section 1
Filling Water Jacket Caution For stacked incubators, completely fill the bottom unit before
filling the top unit in order to maintain the lowest possible center of
gravity.
Turn the power switch On. ADD WATER will appear in the message
center. Press the Silence key to silence the alarm for 15 minutes. If the
water jacket is empty, yet the message center does not display ADD
WATER, then the liquid level switch could be faulty. Call Technical
Services.
Remove the fill port plug and set aside.
For ease of connecting/disconnecting, use the silicone tubing provided to

connect directly to the fill port. See Figure 1-1 and this detail. Do not
install vinyl tubing directly onto the fill port as it may be difficult to
remove. A 3/8” to 3/8” hose connector has been provided to attach lengths
of vinyl tubing to the silicone fill port tubing to reach the water source, or
simply attach the included funnel to manually fill using small containers.
Caution Chlorine is detrimental to stainless steel. Using chlorinated tap

water or additives that contain chlorine will void water jacket warranty! s
For optimum incubator operation, the recommended water type is sterile

distilled Type 2 water with a pH between 7 and 9, and a resistivity
between 50kΩ - 1MΩ-cm (conductivity of 1-20μS/cm). Reverse osmosis
(RO), deionized (DI), and ultrapure Type 1 water
can corrode stainless steel, copper, glass, and other
incubator components over time because high purity
water with low ionic content and high resistivity will
actively pull ions from these materials. If sterile
distilled Type 2 water is not readily available, then
these other water types could be used by adding the Detail 1
necessary amount of a sterile solution of weak sodium
bicarbonate to raise the pH between 7 and 9 and lower the resistivity
between 50kΩ - 1MΩ-cm (conductivity of 1-20μS/cm).
Caution Do not block the water jacket vent! s
Carefully fill the jacket with water. Do not overfill the jacket as the excess
water will pour out through the water jacket vent (see Figure 1-1).
When the jacket is full, the audible alarm will sound a continuous tone for
10 seconds and the alarm condition will be cleared. Refer to Section 4,
Table of Alarms.
Note Model 3100 Series incubators are shipped from the factory with a
rust inhibitor added to 0.2 gallons (0.8 liters) of water in the jacket. The
rust inhibitor must be replenished every 2 years. See Section 5 to drain
water from the jacket and add the correct proportion of rust inhibitor
mixture back into the jacket.

Section 1
Filling Water Jacket Disconnect the silicone tubing from the fill port, and replace the fill port
(continued) plug removed above.
Warning Once the jacket is full, be certain to clean up any water that may
have dripped down the side of the unit or spilled onto the floor during this
process to prevent a possible slip hazard. s
Filling Humidity Pan Caution Use of chlorinated water, or decontamination products containing
chlorine, will deteriorate the stainless steel and cause rust, voiding the
warranty. s
Fill the humidity pan to within 1/2 inch of the top with 0.8 gallons (3
liters) of water, as described in Filling Water Jacket. If the water cannot be
sterilized, a 2% solution of quaternary ammonium can be used as a
disinfectant additive to control bacteria and other organic contaminants, if
desired. Place the pan directly in the center of the incubator floor to
ensure optimum humidity and temperature response.
For applications requiring higher humidity conditions, the pan should be

placed against the left side wall of the incubator. The ductwork has been
designed for this purpose. This will also accelerate humidity recovery after
door openings. Even higher humidity conditions can be achieved by
removing the humidity pan altogether and adding water directly to the
bottom of the incubator chamber, being careful to not fill it any higher
than the bottom edge of the silicone door gasket. Also the chamber gas
sample port (Figure 1-1) may be capped to assist in achieving greater RH.
In some ambients, this may cause condensation to form in the chamber.
Warning Wipe up any excessive condensation to prevent it from dripping

onto the floor creating a possible slip hazard. For stacked incubators, this
may also include the drip channel (just behind the face of the control
panel drawer) on the bottom unit used to direct water away from the
electronics drawer.
Warning When the humidity pan is in place, be certain to clean up any

water that may have spilled in the unit or onto the floor during this
process, to prevent a possible slip hazard. For stacked incubators, this may
also include the drip channel (just behind the face of the control panel
drawer) on the bottom unit, used to direct water away from the electronics
drawer. s
Caution Check the level and change the water frequently to avoid
contamination. Do not allow the water level to fluctuate significantly.
“Dry-outs” will have an adverse effect on the humidity level as well as CO2
calibration. s

Section 1
Connecting CO2 or N2 (O2 Warning High concentrations of CO2 gas can cause asphyxiation! Refer to
System) Gas Supply local EHS policies regarding employee exposure to CO2. Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. s
Warning High concentrations of N2 gas can cause aphyxiation! N2 and

CO2 can displace oxygen levels in a confined atmosphere. Refer to local
EHS policies regarding employee exposure to N2. Oxygen monitors are
recommended for confined areas where concentrations of N2 or CO2 may
displace oxygen. s
Note For customer convenience, 12 feet of 1/4” ID vinyl tubing with two
3/8” hose clamps are included in the shipping materials for connecting the
unit to the gas supply.
The incubator has 1/4” barbed fittings on the back of the cabinet to
connect the gas supply. Refer to Figure 1-13. The fitting is labeled CO2
Inlet #1. The N2 Inlet is also labeled.
a CO2 inletc
Optional Exit for Optional d Power Cord
RS 485 Analog Wiring N2 inlet #1 tank Connector
Remote Air Make-up

a CO2 inletc Convenience
Alarm Inlet
b
#2 tank Electrical
a Outlet
RJ-11 telephone style connectors
b
Used with optional IR sensor
c
Used with optional Gas Guard system
d
Used with optional O2 sensor
Figure 1-13. Back Panel and All Connections
Install 3/8" hose clamps to secure the 1/4" ID tubing to the barbed fittings
on the regulator and the rear of the electronics drawer.
Warning Check for leaks at all connections. s
For units having the CO2 or N2 Gas Guard option, see Section 6.
Warning This incubator is designed to be operated with CO2 gas, or N2 in

those units with an O2 control system. Connecting a flammable or toxic
gas can result in a hazardous condition. Gases other than CO2 or N2
should not be connected to this equipment. s

Section 1
Connecting CO2 or N2 (O2 The CO2 gas supply being connected to the incubator should be industrial
System) Gas Supply (cont.) grade, 99.5% pure. Do not use CO2 gas cylinders equipped with siphon
tubes. A siphon tube is used to extract liquid CO2 from the cylinder,
which can damage the pressure regulator. Consult with your gas supplier
to ensure that the CO2 cylinder does not contain a siphon tube. The N2
gas supply being connected to the incubator should be 99.99% pure. Do
not use liquid nitrogen.
A two-stage pressure regulator is required to be installed on the outlet

valve of the gas cylinder. The high pressure gauge at the tank should have
0-2000 psig range and the low pressure gauge at the incubator inlet should
have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4kPa) maximum for proper performance of
the CO2 or O2 control system.
If higher purity CO2 is desired inside the incubator (greater than 99.5%
pure), the pressure regulator should be constructed with a stainless steel
diaphragm along with specifying the purity of the CO2 from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder. Gas cylinders
should be secured to a wall or other stationary object to prevent tipping.
Position the gas cylinders to allow unobstructed access to the pressure
regulators so that they can be easily turned off in case of an emergency.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations applicable in your area.
Note Stored product should be protected by an activated alarm system

capable of initiating a timely response 24 hours/day. These alarms provide
interconnect for centralized monitoring. See Factory Installed Options,
Section 6 for more information.
Incubator Start-Up Now that the incubator has been properly installed, connected to power,
filled with water, humidity pan filled, and connected to gas supplies,
system setpoints can be entered. The following setpoints can be entered in
set mode: temperature, over temperature, CO2, and O2. To enter Set
Mode, press the Mode key until the Set indicator lights. Press the right
and/or left arrow keys until the proper parameter appears in the message
center. See Chart 1-1 for more detail.
Caution It is the responsibility of the user to validate the proper operation

of each incubator in their specific application(s) with respect to unit
location, operating environment, and settings. s

Section 1
Setting the Operating Models 3110/3111 have an operating temperature (setpoint) range of 10
Temperature to 55°C, Models 3120/3121 at 10 to 50°C, and Models 3130/3131,
3140/3141 at 10 to 45°C. The incubator can only accurately control
temperature as low as +5C above ambient unless it has been installed with
the cooling coil option at the factory which allows the incubator to run at
lower temperatures. See Cooling Coil, Section 6. The incubator is shipped
from the factory with a temperature setpoint of 10°C. At this setting, all
heaters and alarms are turned off.
To change the operating temperature setpoint:
1. Press the Mode key until the Set indicator lights.
2. Press the right arrow until TEMP XX.XC is displayed in the message
center.
3. Press the up/down arrow until the desired temperature setpoint is

displayed.
4. Press Enter to save the setpoint.
5. Press the Mode key until the Run Indicator lights to go to Run mode
or right/left to go to next/previous parameter.
Setting the Overtemp Caution In the event that the heaters are locked on as a result of a failure
Setpoint in the main temperature control, the independent overtemp circuit is
designed as a safety to protect the incubator only. It is not intended to
protect or limit the maximum temperature of the cell cultures or
customer’s equipment inside the incubator if an overtemp condition
occurs. s
The incubator is equipped with an independent circuit that monitors the

air temperature in the cabinet. Should the main temperature control fail,
the overtemp circuit will disconnect power to all heaters when the chamber
temperature reaches the Overtemp setpoint. When the chamber
temperature falls below the Overtemp setpoint, the overtemp circuit will
reconnect power to all heaters. An incubator operating in the overtemp
condition will maintain the chamber temperature approximately 1°C
around the Overtemp setpoint.
The factory setting for the Overtemp is 40°C. It can be set over a range of
temp setpoint +0.5°C to 60°C. If the temperature setpoint is moved above
the Overtemp setpoint, the Overtemp will automatically update to 1.0°C +
the temp setpoint. It is recommended that the Overtemp setpoint be 1°C
over the temp setpoint.

Section 1
Setting the Overtemp To set the Overtemp setpoint:

Setpoint (continued) 1. Press the Mode key until the Set indicator lights.
2. Press the right arrow until OTEMP XX.XC is displayed in message

center.
3. Press the up/down arrow until the desired Overtemp setpoint is

displayed.
Setting CO2 Setpoint All T/C CO2 cells are precalibrated at the factory at 37°C, high humidity,
and 10% CO2. Therefore, if a temperature setpoint of 37°C has been
entered, the humidity pan filled, and the CO2 control is to run between 0-
10% with a T/C CO2 sensor, the CO2 setpoint may be entered
immediately. Otherwise, it is important to allow the unit 12 hours to
stabilize at the temperature setpoint before entering the CO2 setpoint.
All models of the incubator have a CO2 setpoint range of 0.0% to 20.0%.
The incubator is shipped from the factory with a CO2 setpoint of 0.0%.
At this setting, all CO2 control and alarms are turned off.
To change the CO2 setpoint:
2. Press the right arrow until CO2 XX.X% is displayed in message center.
3. Press the up/down arrow until the desired CO2 setpoint is displayed.

Section 1
Setting O2 Setpoint Models 3130, 3131, 3140, and 3141 of the incubator have a built-in O2
control system. The O2 setpoint range is 1.0% to 21.0%. The incubator is
shipped from the factory with a O2 setpoint of 21.0%. At this setting, all
O2 control and alarms are turned off. The gas sample port must be capped
when running controlled O2 levels.
To change the O2 setpoint:
2. Press the right arrow until O2 XX.X% is displayed in the message

center.
3. Press the up/down arrow until the desired O2 setpoint is displayed.

Section 1
Chart 1-1
Set Mode
Press MODE to light SET

36.9
Silence Mode
SET POINTS Enter
Inject % CO
4.9
2
To Set:
Numbers increase
Press MODE to move
Operating to CALIBRATE Press Enter
Mode TEMP XX.X C Enter
Temperature mode
to save setting
Press to return Numbers decrease
to previous parameter
Numbers increase
Over Press Enter

Mode OTEMP XX.X C Enter
Temperature Scroll for Parameters
to save setting
Numbers increase
Press Enter
Percent CO2 Mode CO2 XX.X% Enter
to save setting

Numbers increase
Press Enter
Percent O2 Mode O2 XX.X% Enter
to save setting


Section 2 Calibration
After the unit has stabilized, several different systems can be calibrated. In
the Calibration Mode, the air temperature, CO2 reading, O2 reading, and
RH reading can all be calibrated to reference instruments. To enter
Calibration Mode, press the Mode key until the CAL indicator lights.
Press the right and/or left arrow until the proper parameter appears in the
message center. See Chart 2-1 for more detail.
Calibration frequency is dependent on use, ambient conditions, and

accuracy required. Good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.
Prior to calibration, the user should be aware of the following system

functions. While the unit is in Calibration Mode, all system control
functions will be stopped so that the unit remains stable. Readout of the
system being calibrated will appear as “—-” on the readout displays. If no
keys are pressed for approximately five minutes while in Calibration Mode,
the system will reset to Run Mode so that control functions can be
reactivated.
Caution Before making any calibration or adjustments to the unit, it is

imperative that all reference instruments be properly calibrated. It is the
responsibility of the user to understand the interactive effects of
temperature, CO2%, O2%, and RH% on each other and the independent
reference instruments, especially a Fyrite® sensor. Read and understand all
reference instrument operating manuals before use. Double-check all
values entered into the incubator for accuracy before completing the
calibration and returning the unit to service. s

Section 2
Calibration
Calibrating the Place the calibrated instrument in the center of the chamber. The
instrument should be in the airflow, not against the shelf. Before
Temperature calibration, allow the cabinet temperature to stabilize.
Start-Up - Allow 12 hours for the temperature in the cabinet to stabilize

before proceeding.
Operating Unit - Allow at least two hours after the display reaches setpoint
for the temperature to stabilize before proceeding.
1. Press the Mode key until the CAL indicator lights.
2. Press the right arrow until TMPCAL XX.XC appears in the message
center.
3. Press the up/down arrow to match the display to a calibrated

instrument.
4. Press Enter to store the calibration into memory.
5. Press the Mode key to return to Run, or the right/left arrow to go to

the next/previous parameter.
Calibrating T/C CO2 Models 3110, 3111, 3130 and 3131 have a thermal conductivity (T/C)
System CO2 sensor. Thermal conductivity of the incubator atmosphere is not only
effected by the quantity of CO2 present, but also by the air temperature
and the water vapor present in the incubator atmosphere. In monitoring
the effects of CO2, air temperature and absolute humidity must be held
constant so any change in thermal conductivity is caused only by a change
in CO2 concentration.
Any changes to the temperature setpoint and/or humidity level control

method (humidity pan location, capping ports, HEPA filter type) will
necessitate a recalibration of the CO2 control.
Some T/C CO2 sensors go through an aging period, especially on new

installations. Calibration should be checked on a weekly basis, and
adjusted as necessary. When stabilization occurs, checks can become less
frequent.

Section 2
Calibration
Calibrating T/C CO2 Start-up - The CO2 sensor has been calibrated at the factory for 37°C.
Allow temperature, humidity, and CO2 levels in the chamber to stabilize
System (continued) at least 12 hours before checking the CO2 concentration with an inde-
pendent instrument.
Presently operating - Make sure the chamber doors are closed. Allow at
least 2 hours after the temperature and CO2 displays reach their set-
points for chamber atmosphere stabilization.
1. Measure the CO2 concentration in the chamber through the gas

sample port with an independent instrument. Take several readings to
ensure accuracy.
3. Press the right arrow until CO2CAL XX.X% is displayed in the

message center.
4. Press the up/down arrow to change the display to match the

independent instrument.
5. Press Enter to store calibration.
6. Press the Mode key to return to Run Mode, or the right or left arrow
keys to go to the next/previous parameter.
Calibrating Infra-Red Models 3120, 3121, 3140 and 3141 have an infra-red CO2 sensor. Infra-
CO2 System red CO2 sensors are not affected by chamber atmosphere, temperature or
humidity. However, the light detector in the sensor is affected by wide
temperature changes. Therefore, changing temperature setpoints could
necessitate a recalibration of the IR CO2 sensor before use. Chamber
temperature should be allowed to stabilize before checking CO2
concentrations with an independent instrument, especially on start-up.
All models equipped with an IR CO2 sensor have an automatic calibration

that occurs every 24 hours, and lasts for 5 to 6 minutes. During automatic
calibration, the CO2 display is blanked out and HEPA filtered room air is
pumped through the CO2 sensor. A new CO2 calibration value is stored in
memory for use as the 0.0% CO2 reference point. The keypad/ control
panel is locked during calibration, with the message center reading CO2
AUTO CAL.

Section 2
Calibration
Calibrating Infra-Red Startup- Allow the temperature and the CO2 of the cabinet to stabilize at
least 12 hours before proceeding.
CO2 System (cont.)
Operating Unit - Allow CO2 to stabilize at least 2 hours at setpoint before
proceeding.
To ensure accurate calibration, the unit will not allow CO2 to be spanned
below 3%. If the cabinet does not contain at least 3% CO2, increase the
setpoint and allow the unit to stabilize before completing this procedure.
1. Measure the CO2 concentration in the chamber through the gas

sample port with an independent instrument. Take several readings to
ensure accuracy.
3. Press the right arrow until IR CAL XX.X% appears in the message
center.
4. Press the up/down arrow to adjust the display to match the

independent instrument reading.
6. After Enter is pressed, the unit will go into a calibration cycle that lasts
5 to 6 minutes. Control panel is locked during this calibration cycle.
7. Press the Mode key to return to Run Mode.
Calibrating the O2 Models 3130, 3131, 3140 and 3141 have an O2 control sensor. The sensor
is a fuel cell that puts out a linear millivolt signal based on O2 content of
System the chamber. The life expectancy for an O2 sensor fuel cell is
approximately 5 years (specified at an oxygen concentration of 20.7% and
ambient temperature of 20°C) even if the unit is not in use. During
normal incubator operation, exposure to lower concentrations of oxygen
increases the life expectancy, whereas increased ambient temperatures
decreases it. The cumulative effects of these conditions over time are
unpredictable, but the unit is able to measure the remaining life of the O2
sensor fuel cell based on its voltage output when the system is calibrated
using the 20.7% method and display a sensor fault as required (Section 4);
thus, it is important to calibrate the system using this method before each
test experiment, or at least every 6 months.

Section 2
Calibration
Calibrating the O2 There are two methods available to calibrate the O2 system.
System (continued) • The preferred method calibrates the system to the known ambient O2
value of 20.7% and checks the life of the sensor. This method should
be used whenever a new O2 sensor fuel cell is installed.
• The second method available allows the system to be calibrated to an

independent reference instrument by entering an offset. This method
should be used whenever higher accuracy is required as it takes into
account the effects of the desired chamber temperature setpoint on the
O2 sensor fuel cell output; however, this method does not measure its
remaining life.
O2 Calibration at 20.7% 1. Press the Mode key until the CAL indicator lights.
2. Press the right arrow until the display reads O2 [email protected]%.
3. Press Enter.
4. OPEN DOOR appears on the display. Open the outer and inner
doors.
5. The display reads CALIBRATING.
6. When calibration is complete, approximately 2 minutes, an audible

tone sounds and the display returns to O2 [email protected]%.
7. The O2 display will change to 20.7.
8. Press the Mode key to return to Run.
A new O2 span value is stored in memory for use as the 20.7% O2

reference point. The keypad/control panel is "locked-up" during
calibration.

Section 2
Calibration
O2 Offset Calibration Startup - Allow the cabinet to stabilize at least 12 hours before proceeding.
Operating Unit - Allow O2 to stabilize at least 2 hours at set point before
proceeding.
1. Measure the O2 concentration in the chamber through the gas sample

port with an independent instrument. Take several readings to ensure
accuracy.
3. Press the right arrow until O2 OFFS XX.X% appears in the message
center.
4. Press the up/down arrow to adjust display to independent instrument

reading.
Calibrating Relative All Model 3100 Series incubators can be equipped with an optional direct
Humidity (RH) readout relative humidity sensor. This is a readout only of the chamber
relative humidity. It does not provide any control of the relative humidity
in the cabinet.
Place the calibrated instrument in the center of the chamber. The

instrument should be in the airflow, not against the shelf. Before
calibration, allow the cabinet temperature to stabilize.
Startup - Allow 12 hours for the relative humidity and temperature in the
chamber to stabilize before proceeding.
Operating Unit - Allow at least 2 hours after temperature display reaches
setpoint for relative humidity to stabilize before proceeding.
2. Press the right arrow key until RH CAL XX% appears in message
center.
3. Press the up/down arrow to match the display to the independent

instrument.
4. Press Enter to store the calibration.

Section 2
Calibration
Calibrating Relative If a reliable RH measuring device is not available, the display may be
calibrated to a typical level. This calibration method should be accurate to
Humidity (RH) cont. within 5%.
1. Follow the RH stabilization periods outlined above.
2. With a full humidity pan and stable temperature, the relative humidity
in the chamber will be 95%.
3. Using Steps 3-5 of the relative humidity sensor adjustment above,

adjust the display to 95%.

Section 2
Calibration
Chart 2-1
Calibrate Mode
Press MODE to light CAL:

36.9
Enter
Silence Mode CALIBRATE Inject % CO2
Scroll for Parameters 4.9
To Calibrate:
Numbers increase
Press MODE to move
Operating to SYS CONFIG mode Press Enter to
Mode Enter
TMPCAL XX.XC save the setting
Temperature Scroll for Parameters

Numbers increase
Zero CO2 Enter

Press Enter to
Mode CO2CAL XX.XC
(when Thermal Conductivity save the setting
sensor is in use) Numbers decrease
Press to return
Numbers increase
Span Co2 Enter

Press Enter to
Mode
IR CAL XX.X% save the setting
(when IR sensor is in use) Scroll for Parameters

Numbers increase
Percent O2 @ 20.7% Mode Enter

Press Enter to
O2 [email protected]%
save the setting
(when O2 option is in use) Scroll for Parameters

Numbers increase
Percent O2 Mode Enter

Press Enter to
O2 OFF XX.X%
save the setting
(when O2 option is in use) Scroll for Parameters
Press to return
Numbers decrease
Numbers increase
Percent RH Mode Enter

Press Enter to
RH CAL XX%
(when RH option is in use) Scroll for Parameters
save the setting

Section 3 Configuration
There are many features available in Configuration Mode that allow
custom setup of the incubator. These features are described below. All
features may not be necessary in all applications, but are available if
needed. To enter Configuration Mode, press the Mode key until the
Config indicator lights. Press the right or left arrow until the appropriate
parameter appears in the message center. See Chart 3-1 for more detail.
Turning the Audible The audible alarm can be turned on or off. The factory setting is ON.
Alarm ON/OFF 1. Press the Mode key until the Config indicator lights.
2. Press right arrow until AUDIBLE XXX is displayed in message center.
3. Press the up/down arrow to toggle Audible ON/OFF.
4. Press Enter to save the setting.
5. Press the Mode key to return to Run Mode or right/left to go to

next/previous parameter.
New HEPA Filter When the REPLACE HEPA reminder is displayed and the visual alarm
flashes, the specified time has elapsed and the HEPA filter should be
replaced. Follow the HEPA Filter Maintenance procedures in Section 5.
The factory setting is 182 days (calculated from REPL HEPA XX setting
of 6 months).
1. Press the Mode key until the Config indicator lights.
2. Press right arrow until NEW HEPA XXX is displayed in message

center.
Note Do not press Enter if only viewing remaining days before specified
filter replacement time runs out.
3. Press Enter to restart the timer and clear the REPLACE HEPA alarm.
This new number is the remaining days before the specified filter
replacement time runs out. For example, if 12 months was chosen in
the REPL HEPA XX message screen, the NEW HEPA number would
be 365 days.

Section 3
Configuration
Setting the REPLACE A HEPA filter replacement timer can be set for a specific amount of time,
from 1 to 12 months of actual unit running time. Time will not accrue
HEPA Filter Reminder when the unit is turned off. When the allotted time has run out,
REPLACE HEPA appears in the display and the visual alarm flashes. The
factory setting is 6 months.
2. Press the right arrow until REPL HEPA XX is displayed.
3. Press the up/down arrow to choose the number of months desired.
4. Press Enter to save the number.

Note After the reminder has been set, check the allotted time remaining by
going to Config Mode, then pressing the right arrow until NEW HEPA
XXX displays. This number is the remaining days before the specified filter
replacement time runs out. For example, if 12 months was chosen in the
REPL HEPA XX message screen, the NEW HEPA number would be 365
days. s
Setting an Access A three-digit Access Code can be entered to avoid unauthorized personnel
from changing the setpoints, calibration, or configuration. A setting of
Code 000 will bypass the access code. The factory setting is 000.
2. Press the right arrow until ACC CODE XXX is displayed in the
message center.
3. Press the up/down arrow to change the access code.
4. Press Enter to save the access code.


Section 3
Configuration
Setting Low Temp The low temp alarm limit (tracking alarm) is the deviation from the
temperature setpoint, which will cause a low temp alarm. The low temp
Alarm Limit alarm is variable from 0.5° below setpoint to 5.0° below setpoint. The
factory setting is 1.0° below setpoint. A minus sign (-) in the display
indicates that the alarm setting is below the setpoint.
2. Press the right arrow until TMP LO -X.XC is displayed in the

message center.
3. Press the up/down arrow to change the low temp alarm limit.
4. Press Enter to save the low temp alarm limit.
5. Press the Mode key to return to Run mode or right/left to go to

Setting High Temp The high temp alarm limit (tracking alarm) is the deviation from the
temperature setpoint that will cause a high temp alarm. It should be noted
Alarm Limit that this varies from the Overtemp setpoint, in that the Overtemp setpoint
configures an independent system that monitors temperature and shuts
down the system heaters if necessary. The high temp alarm limit is simply
set to enable an audible and visual alarm that notifies the user of a
problem. See Section 1, Setting the Overtemp Setpoint. The high temp
alarm is variable from 0.5° above setpoint to 5.0° above setpoint. The
factory setting is 1.0° above setpoint.
2. Press the right arrow until TMP HI X.XC is displayed in the message
center.
3. Press the up/down arrow to change the high temp alarm limit.
4. Press Enter to save the high temp alarm limit.


Section 3
Configuration
Enabling Temp Alarms High and Low temperature alarms can be programmed to trip the remote
alarm contacts. A setting of ON will cause this, a setting of OFF will not
to Trip Contacts allow temp alarms to trip the contacts. The factory setting is ON.
2. Press the right arrow until TEMP RLY XXX is displayed in the
message center.
3. Press the up/down arrow to toggle the setting ON/OFF.
4. Press Enter to save the setting

Setting Low CO2 The low CO2 alarm limit (tracking alarm) is the deviation from the CO2
Alarm Limit setpoint that will cause a low CO2 alarm. The setpoint is variable from
0.5% CO2 below setpoint to 5.0% CO2 below setpoint. The factory
setting is 1.0% CO2 below setpoint. A minus (-) in the display indicates
that the alarm setting is below the setpoint.
1. Press the Mode Key until the Config indicator lights.
2. Press the right arrow until CO2 LO -X.X% is displayed in the

message center.
3. Press the up/down arrow to change the low CO2 alarm limit.
4. Press Enter to save the low CO2 alarm limit.

Setting High CO2 The high CO2 alarm limit (tracking alarm) is the deviation from the CO2
Alarm Limit setpoint that will cause a high CO2 alarm. The setpoint is variable from
0.5% CO2 above setpoint to 5.0% CO2 above setpoint. The factory
setting is 1.0% CO2 above setpoint.

Section 3
Configuration
Setting High CO2 1. Press the Mode key until the Config indicator lights.
Alarm Limit (cont.) 2. Press the right arrow until CO2 HI X.X% is displayed in the message
center.
3. Press the up/down arrow to change the high CO2 alarm limit.
4. Press Enter to save the high CO2 alarm limit.

Enabling CO2 Alarms High and Low CO2 alarms can be programmed to trip the remote alarm
to Trip Contacts contacts. A setting of ON will cause this; a setting of OFF will not allow
CO2 alarms to trip the contacts. The factory setting is ON.
2. Press the right arrow until CO2 RLY XXX is displayed in the message
center.

Setting Zero #, Span # If a new T/C CO2 sensor is being installed, the two numbers on the
for T/C CO2 Sensors factory installed sticker on the T/C cell must be entered to calibrate the
CO2 in the unit.
Note For the technician’s convenience, a second label containing the two
numbers is supplied with the new T/C cell and should be applied to cover
the original label, inside the electronics drawer.

Section 3
Configuration
Setting Zero #, Span # 1. Press the Mode key until the Config indicator lights.
for T/C Sensors (cont.) 2. Press the right arrow until T/C ZR# XXXX is displayed in the message
center.
3. Press the up/down arrow to change the zero number to match the
sticker.
Note The '-' symbol denotes the 'minus sign' which is displayed as
required by scrolling the zero number through 0.
5. Press the right arrow until T/C SP# XXXX is displayed in the message
center.
6. Press the up/down arrow to change the span number to match the
sticker.
Note The '-' symbol denotes the 'minus sign' which is displayed as
required by scrolling the zero number through 0.
Note Pressing Enter will erase any CO2CAL offset value previously
entered into the Calibration menu.

Setting a Low RH On units that have the RH option installed, a low RH alarm limit may be
Alarm Limit entered. The low RH alarm limit is the %RH in the cabinet that will
cause a low RH alarm. The setpoint is variable from setpoint 0 to 90
%RH. The factory setting is 0% RH, which disables the alarm.
2. Press the right arrow until RH LO XX% is displayed in the message

center.
3. Press the up/down arrow to change the RH low alarm limit.
4. Press Enter to save the RH low alarm limit.


Section 3
Configuration
Enabling RH Alarms The low RH alarm can be programmed to trip the remote alarm contacts.
A setting of ON will cause this, a setting of OFF will not allow the RH
to Trip Contacts alarm to trip the contacts. The factory setting is ON.
2. Press the right arrow until RH RLY XXX is displayed in the message
center.

Setting a Low O2 On models with a O2 control system, O2 alarms may be configured. The
Alarm Limit low O2 alarm limit (tracking alarm) is the deviation from the O2 setpoint
that will cause a low O2 alarm. The setpoint is variable from 0.5% O2
below setpoint to 5.0% O2 below setpoint. The factory setting is 1.0% O2
below setpoint. A minus (-) in the display indicates that the alarm setting
is below setpoint.
2. Press right arrow until O2 LO -X.X% is displayed in message center.
3. Press the up/down arrow to change the low O2 alarm limit.
4. Press Enter to save the low O2 alarm limit.


Section 3
Configuration
Setting a High O2 On models with a O2 control system, O2 alarms may be configured. The
high O2 alarm limit (tracking alarm) is the deviation from O2 setpoint that
Alarm Limit causes a high O2 alarm. The setpoint is variable from 0.5% O2 above
setpoint to 5.0% O2 above setpoint. The factory setting is 1.0% O2 above
setpoint.
2. Press right arrow until O2 HI X.X% is displayed in message center.
3. Press the up/down arrow to change the high O2 alarm limit.
4. Press Enter to save the high O2 alarm limit.

Enabling O2 Alarms to High and Low O2 alarms can be programmed to trip the remote alarm
contacts. A setting of ON will cause this, a setting of OFF will not allow
Trip Contacts the O2 alarms to trip the contacts. The factory setting is ON.
2. Press right arrow until O2 RLY XXX is displayed in message center.


Section 3
Configuration
Enabling Temp/RH to On units that are equipped with the RH option, the upper seven-segment
display on the control panel can be configured to display Temp
be Displayed continuously, RH continuously, or toggle between Temp and RH. If the
units does not have RH, the upper display will always display temperature.
If temperature is set to ON and the RH is set OFF, temperature will be
displayed continuously. If temperature is set to OFF and RH is set to ON,
RH will be displayed continuously. If both are turned ON, the display
will toggle between the two. The factory setting will default to toggle
mode if the RH option is present.
2. Press the right arrow until DISP TMP XXX or DISP RH XXX is
displayed in the message center.

Enabling CO2/O2 to be On models that are equipped with the O2 system, the lower seven-segment
Displayed display on the control panel can be configured to display CO2
continuously, O2 continuously, or toggle between CO2 and O2. If the
units does not have O2, the lower display will always display CO2. If CO2
is set to ON and the O2 is set OFF, CO2 will be displayed continuously.
If CO2 is set to OFF and O2 is set to ON, O2 will be displayed
continuously. If both are turned ON, the display will toggle between the
two. The factory setting will default to toggle mode if the O2 system is
present.
2. Press the right arrow until DISP CO2 XXX or DISP O2 XXX is
displayed in the message center.


Section 3
Configuration
Selecting a Primary On units equipped with the Gas Guard option, a primary tank can be
selected. The primary tank can be either Tank 1 or Tank 2. Factory
Tank (Gas Guard) setting is Tank 1.
2. Press right arrow until TANK SEL X is displayed in the message

center.
3. Press the up/down arrow to toggle the setting between 1 and 2.

Disabling the Gas On units equipped with the Gas Guard option, the Gas Guard system may
Guard System be turned ON, or OFF if it is not in use. The factory setting is ON.
2. Press right arrow until GAS GRD XX is displayed in message center.

Setting a RS485 On units with the RS485 option, direct communication with Model 1535
Address (1535 Only) alarm system can be established. Each piece of equipment connected to
the 1535 must have a unique address. An address of 0-24 can be entered
for the incubator. A setting of 0 is a non-communication address that the
1535 ignores. Factory setting for the RS485 address is 0.
2. Press right arrow until 485 ADDR XX is displayed in message center.
3. Press the up/down arrow to change the RS485 address.
4. Press Enter to save the RS485 address.


Section 3
Configuration
Chart 3-1
Configure Mode
Press MODE to light CONFIG

36.9
Enter
Silence Mode SYS CONFIG Inject % CO 2
Scroll for Parameters 4.9
To Configure:
Turn ON audible alarm
Press MODE to return
to RUN mode Mode Enter Press Enter to
AUDIBLE XXX
Audible ON/OFF Scroll for Parameters
save the setting
Press to return Turn OFF audible alarm
Mode
NEW HEPA XXX Enter Press Enter to
NEW HEPA Filter Scroll for Parameters save the setting
Press to return
Numbers increase
Press Enter to
REPLACE HEPA Mode
REPL HEPA XX Enter
save the setting
Timer Press to return
Numbers decrease
Numbers increase
Mode Enter
Press Enter to
Access Code ACC CODE XXX
save the setting

Numbers increase
Low Temp Mode

TMP LO XX.XC Enter Press Enter to
Alarm Limit Scroll for Parameters
save the setting
Numbers increase
High Temp Mode
TMP HI XX.XC Enter
Press Enter to
Alarm Limit save the setting

continue on next page

Section 3
Configuration
Configure Mode, Chart 3-1, Page 2 of 4

refer to previous page
To Configure: Enable relay contacts (ON)

during temp alarms
Enter
Press Enter to
Mode
Temp Relay ON/OFF TEMP RLY XXX save the setting
Press to return Disable relay contacts (OFF)
to previous parameter during temp alarms
Numbers increase
Mode Enter Press Enter to

CO2 % Low Limit CO2 LO X.X %
save the setting
Numbers increase
Press Enter to
Mode Enter
CO2 % High Limit CO2 HI X.X % save the setting
Enable relay contacts (ON)
during CO2 alarms
Press Enter to
CO2 Relay ON/OFF Mode CO2 RLY XXX Enter
save the setting

to previous parameter during CO2 alarms
Numbers increase
Enter
Press Enter to
Mode T/C ZR# -XXXX
T/C Zero Number save the setting
Numbers increase
Press Enter to
Enter
T/C Span Number Mode T/C SP# +XXXX save the setting

Numbers increase
Mode
RH LO X% Enter Press Enter to
RH Low Alarm Limit Scroll for Parameters
save the setting
Press to return
to previous parameter Numbers decrease
Enable relay contacts (ON)

during RH alarm
RH RLY XXX
RH Relay ON/OFF Scroll for Parameters
save the setting
to previous parameter during RH alarm
refer to next page

Section 3
Configuration

Section 3
Configuration
Configure Mode, Chart 3-1, Page 4 of 4
refer to previous page
To Configure:
Tank 1 prime
Tank Select Mode Enter

Press Enter to
TANK SEL X save the setting
Press to return Tank 2 prime

Numbers increase
Press Enter to
Mode Enter
GAS GRD XXX save the setting
Gas Guard ON/OFF Scroll for Parameters

Numbers increase

485 ADDR XX
RS485 Scroll for Parameters
save the setting

Section 4 Alarms
The Model 3100 Series incubator alarm system is shown in the table
below. When an alarm is active, the message appears in the LED message
center. Pressing Silence disables the audible alarm for the ringback period.
However, the visual alarm continues until the incubator returns to a
normal condition. The alarms are momentary alarms only. When an alarm
condition occurs and then returns to normal, the incubator automatically
clears the alarm condition and the message center.
When multiple alarm conditions occur, active messages are displayed in

the message center one at a time, updating at 5 second intervals. Pressing
Silence during multiple alarms causes all active alarms to be silenced and to
ring back in 15 minutes.

Section 4
Alarms
Table 4-1. Alarms

Description Message Delay Ringback Relay
No alarm condition exists SYSTEM OK/CLASS 100 --- --- ---
CO2 System Auto Calibrating CO2 AUTO CAL --- --- ---
Temp > Otemp Setpoint (See Sect. 1) SYS IN OTEMP 0 min 15 min Yes
Temp Control Sensor Fault (See Sect 4) TSNSR1 ERR 0 min 15 min No
Over Temp Sensor Fault (See Sect 4) TSNSR2 ERR 0 min 15 min No
CO2 Sensor Fault (See Sect 4) CO2 SNSR ERR 0 min 15 min No
O2 Sensor Fault (O2 option, see Sect 4) O2 SNSR ERR 0 min 15 min No
O2 Sensor Low (O2 option, see Sect 4) REPL O2 SNSR 0 min --- * No
CO2 Sensor cannot be calibrated (IR option, Sect. 4) REPL IR SNSR 0 min 15 min No
Replace HEPA filter reminder – set time expired (See Sect. 3) REPLACE HEPA 0 min --- * No
Water low in jacket (See Sect. 1) ADD WATER 0 min 15 min No
Inner Door is Open (See Sect. 4) DOOR IS OPEN 15 min 15 min No
CO2 > CO2 High Tracking Alarm CO2 IS HIGH 15 min 15 min Programmable
CO2 < CO2 Low Tracking Alarm CO2 IS LOW 15 min 15 min Programmable
TEMP > TEMP High Tracking Alarm TEMP IS HIGH 0 min 15 min Programmable
TEMP < TEMP Low Tracking Alarm TEMP IS LOW 15 min 15 min Programmable
O2 > O2 High Tracking Alarm (O2 option) O2 IS HIGH 15 min 15 min Programmable
O2 < O2 Low Tracking Alarm (O2 option) O2 IS LOW 15 min 15 min Programmable
RH < RH Low Limit Alarm (RH option) RH IS LOW 30 min 15 min Programmable
CO2 Auto-Zero Fault (IR option, see Sect. 4) IR AUTOZ ERR 0 min 15 min No
Tank 1 is low, switch to Tank 2 (Gas Guard) TANK1 LOW 0 min --- No
Tank 2 is low, switch to Tank 1 (Gas Guard) TANK2 LOW 0 min --- No
Both tanks are low (Gas Guard) TANK 1 and 2 LOW 0 min 15 min No
- All alarm delays and ringback times are +/- 30 seconds -
*Visual Alarm Only
Inner Door Open When the incubator’s inner door is opened, heat and CO2 injection are
Alarm disabled. The door must be securely latched for heat and CO2 injection to
resume after a door opening. If the door is latched yet the display still
shows DOOR IS OPEN, or if the door is unlatched and the display does
not show DOOR IS OPEN, the door switch could be faulty. Call
Technical Services.
Sensor Fault Alarms The microprocessor in the incubator continually scans all available sensors,
except the O2 (see Section 4), to ensure proper operation. Should an error
be detected, the incubator sounds an alarm and displays the appropriate
message. If such an alarm occurs, contact your local distributor or the
Technical Services department.

Section 4
Alarms
TSNSR1 ERR If the microprocessor determines that the temperature control precision
thermistor is under-range or over-range, TSNSR1 ERR appears in the
display, the visual alarm flashes, the audible alarm sounds, and the
chamber and door heaters are disabled.
TSNSR2 ERR If the microprocessor determines that the over temperature precision
thermistor is under-range or over-range, TSNSR2 ERR appears in the
display, the visual alarm flashes, the audible alarm sounds. If the
independent over temperature protection circuit determines that the
thermistor is over-range, SYS IN OTEMP alternates in the display and the
chamber and door heaters are disabled.
REPL O2 SNSR On units equipped with the O2 system, the microprocessor checks the
remaining life of the O2 sensor whenever O2 calibration @ 20.7% is
performed. After the O2 sensor declines to less than 43% (typical) of its
life expectancy, REPL O2 SNSR appears in the display and the visual
alarm flashes. This alarm alerts the user to replace the O2 sensor at the
earliest convenience (Section 5). The unit will continue to function for
some length of time.
O2 SNSR ERR After the O2 sensor declines to the point that control cannot be accurately
performed, an O2 SNSR ERR appears in the display, the visual alarm
flashes, the audible alarm sounds, and the chamber and O2 control is
disabled. The O2 control and alarms can be disabled by changing the
setpoint to 21.0% (Section 1) or replacing the O2 sensor fuel cell (Section
5).
CO2 SNSR ERR If the cables or connectors between the main microprocessor board and the
CO2 sensor, or between the CO2 board and the sensor head on I/R CO2
units become loose or disconnected, the CO2 SNSR ERR alarm will
occur.
I/R Units On I/R incubators, the red light on the I/R module (see Figure 5-8 for the
module circuit board location) is lit continuously if communication is lost
between the CO2 board and the sensor head. The CO2 display is locked at
00.0 without injection. Turning the incubator off and on will not clear the
alarm. Only proper connections of all the components corrects the alarm.

Section 4
Alarms
IR AUTOZ ERR On incubators equipped with an IR CO2 control system, calibration is

performed automatically once every 24 hours using the Auto Zero feature.
If a CO2 correction of more than 0.45% is detected then the Auto Zero
cycle is aborted and the IR AUTOZ ERR appears in the display, the visual
alarm flashes, and the audible alarm sounds. The unit will continue to
function using the last valid CO2 correction value. The error may be
cleared by performing the IR CO2 control system calibration (see Section
2) or waiting 24 hours for the next Auto Zero cycle.
Possible problems which will cause this alarm are:
• Auto Zero pump, orifice, filter or tubing will not allow air to the
sensor.
Possibilities are:
~ Defective or electrically disconnected air pump
~ Kinked auto zero vinyl tubing
~ Disconnected tubing between air pump and sensor
~ Plugged filter or orifice on auto zero assembly
~ Defective auto zero circuit
• Cabinet temperature has been increased significantly from a previous

setpoint. (For example, the unit was calibrated and operating at 30°C
and the setpoint is increased to 50°C) In this instance, calibrating the
CO2 will correct the alarm.
• There is high background CO2 in the laboratory. This could be from

leaks in the tank, regulator or vinyl CO2 tubing.
• High CO2 sensor calibration drift occurred. This requires replacement

of the sensor.
WARNING Do not disassemble the IR CO2 sensor as there are no user-

serviceable adjustments.
REPL IR SNSR If the Auto Zero feature cannot properly calibrate the sensor, then REPL
IR SNSR appears in the display, the visual alarm flashes, the audible alarm
sounds, and the chamber CO2 control disabled. The CO2 control and
alarms can be disabled by changing the setpoint to 0.0% (Section 1) or
replacing the IR CO2 sensor. Call Technical Services.

Preventive Maintenance
PREVENTIVE MAINTENANCE
Incubators
Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your unit
functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and efficiency, it
is recommended the unit be checked and calibrated periodically by a qualified service technician.
The following is a condensed list of preventive maintenance requirements. See the specified section of the operating manual for further details.
We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive Maintenance
or Extended Warranties, please contact Technical Services.
Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required.
Tips for all incubators:

Do NOT use bleach or any disinfectant that has high chlorine content.
Use water with characteristics as described in Filling Water Jacket.
Avoid spraying cleaner on the CO2 sensor.
Do not use powdered gloves for tissue cultures.
Action Daily Weekly Monthly 6 Yearly 2

Months years
Check CO2 and/or N2 tank levels (tanks without optional gas guard monitor)
Inspect the inner and outer door latches and hinges for proper operation and excessive
mechanical wear; inspect the line cord insulation and inner door silicone gasket for
chips and cracks. Replace as required.
Check water level in humidity pan, ½” from top. See “Filling the Humidity Pan”.
Check proper blower motor operation (air flow, noise, and vibration)
Verify and document CO2, humidity and temperature calibration, as applicable. See
Calibration section.
Verify O2 calibration, as applicable. See Calibration section
Perform a complete decontamination procedure. Wipe down interior, shelves and side Before first use, after every service call and between experiments
ducts with disinfectant. Change or clean blower wheel and scroll. Clean top duct. to prevent cross contamination
Clean humidity pan. Rinse everything well with sterile water. See “Cleaning the
More frequent decontamination may be required, depending on
Interior, “Cleaning the Humidity Pan”.
use and environmental conditions
Change HEPA and gas filters, if applicable (or as needed). See “Installing Access Port
Filter, “Installing Air Sample Filter”, “Installing HEPA Filter”.
Replenish rust inhibitor in water jacket. See “Adding/Replenishing Rust Inhibitor”.

Section 5 Routine Maintenance
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users must check with the
manufacturer that the proposed method will not damage the equipment. s
Warning It is the responsibility of the user to immediately clean up after

all accidental spills of hazardous materials. Be certain to follow local EHS
policies with regards to personal protective equipment, cleaning, and
disposal.
Cleaning the Interior Use an appropriate disinfectant, such as quaternary ammonium. All
articles and surfaces must be thoroughly cleaned, rinsed and rough-dried.
Warning Alcohol, even a 70% solution, is volatile and flammable Use it

only in a well ventilated area that is free from open flame. If any
component is cleaned with alcohol, do not expose the component to open
flame or other possible hazard. Allow the alcohol to fully dry before
turning power on. s
Warning Do not spray the T/C sensor with flammable solutions. The
internal temperature of the CO2 sensor is approximately 150°C when the
unit is in operation. Allow sufficient time for the sensor to cool before
cleaning. s
Caution Do not use strong alkaline or caustic agents. Stainless steel is

corrosion resistant, not corrosion proof. Do not use solutions of sodium
hypochlorite (bleach), as they may also cause pitting and rusting. s
Note If condensation is visible on the ceiling of the chamber, yet the

message center does not display ADD WATER, then the liquid level
switch could be faulty. Call Technical Services.
Warning If the unit has been in service, disconnect the power cord from
both the unit and the power source, turn off all gas regulators, and allow
the unit to cool before disinfecting.

Section 5
Routine Maintenance
Cleaning the Interior 1. Remove the shelves, access port

filter and side duct sheets.
(continued) Remove the temperature sensors
and the air sample filter tubing
from the back of the blower
scroll. If unit is equipped with
the optional RH sensor,
unfasten it from the clip on the
top duct. See Figure 5-1.
Figure 5-1. Sensor and Filter Locations
2. Remove the filter from the air
sample filter tubing. Carefully pull down and remove the HEPA filter.
3. Remove the wingnuts securing the top duct to the interior. Carefully
slide the top duct down and off the temperature sensor, air sample
filter tubing (and RH sensor, if applicable).
4. Wash the shelves, ducts, wingnuts and stopper with disinfectant and
rinse with sterile water. Option: Autoclave shelves, ducts and wingnuts.
5. Remove the blower scroll by first pushing the black lever clip closest to
you toward the scroll. Then turn the scroll to the right to disengage it
from the blower scroll plate. Some manipulation may be required as
the alignment holes are keyhole-shaped.
6. Remove the remaining wingnut, then pull down on the blower wheel.
If a new wheel and scroll are to be used, discard the old ones per local
EHS policies and procedures. If the old ones are to be reused, wash all
parts with disinfectant and rinse with sterile water.
7. Remove the blower scroll plate by first pushing the black lever clip
toward the chamber ceiling. Then turn the plate to the left to
disengage it from the alignment keyholes. Clean as above, or autoclave.
8. Wash the cabinet interior with disinfectant starting at the top and
working down. Wash the inner door both inside and out. The cabinet
and door must be rinsed with sterile water until the disinfectant has
been removed. After the cabinet has been rinsed,
spray with 70% alcohol. Align Blower
flat sides Motor
9. Reinstall the blower scroll plate by aligning it with Shaft
the larger end of the keyholes and turning it to the
left to lock it on. Pull the black lever clip
downward from the ceiling.
Blower Wheel
10. Install the blower wheel onto the motor shaft, Collar
aligning the d-shaped flat sides of each. See Figure
5-2. Secure the blower wheel with the wingnut. Figure 5-2. Align
Make sure the wheel turns freely.

Section 5
Routine Maintenance
Cleaning the Interior 11. Locate the blower scroll over the blower wheel into the larger end of
the keyholes on the scroll plate. Turn the scroll to the right to lock it
(continued) into the keyholes. Pull the black lever clip closest to you toward the
front of the unit.
12. Install the top duct by feeding the

Top Duct
temperature sensors, air sample
tubing (and RH sensor, if
Grommets
applicable) through the appropriate
Temp
holes in the duct as it is raised to RH Sensor Sensors
the top of the chamber. Be careful (optional)
Air Sample
not to pull the grommets through Filter Tubing
the duct. See Figure 5-3.
Figure 5-3. Feed Through Holes
13. Locate the mounting studs and blower scroll into the appropriate holes
in the top duct and install the wingnuts to secure the duct.
14. Install the air sample filter front of unit

onto the top duct tubing.
15. Carefully pull the

temperature sensors and
air sample filter tubing CO2
down until they can be inject port
inserted approximately 1 Air sample
inch into the appropriate filter tubing
holes in the back of the Temp sensor
blower scroll. If applicable,
place the optional RH Temp sensor
connection Air sample
sensor into the filter tubing
corresponding clip on the connection
top duct. See Figure 5-4. Figure 5-4. Underside View of Chamber
Ceiling
16. Install the HEPA Filter.
17. Wipe down the interior of the incubator with 70% alcohol, including
the glass door and gasket.
Caution Do not spray or wipe the sensors. s
17. Install the left and right ducts, and the access port stopper with filter,
spraying each with 70% alcohol (do not saturate).
18. Install the shelves and spray with 70% alcohol.
19. Plug the incubator in and turn the power switch on. Allow the unit to
run with the chamber empty, heat only, for 24 hours before returning
to service.

Section 5
Routine Maintenance
Cleaning Cabinet 1. Use a lint-free cloth dampened in mild soapy water to clean the
exterior, especially the door handle. Do not use any liquids (including
Exterior spray cleaners) on the control panel; instead clean with a dry
microfiber cloth.
2. Wipe the surfaces (except control panel) twice using a clean cloth
dampened in clear water.
3. Wipe dry using a clean cloth.
Cleaning Humidity Clean the humidity pan with soap and water and a general use laboratory
disinfectant. Rinse with sterile water and spray with 70% alcohol. The
Pan humidity pan may be autoclaved.
Reversing the Door For side-by-side operation or changing lab layouts, the inner and outer
Swing doors are field-reversible. The procedure is written from the prospective of
changing the door swing from a left-hand to a right-hand swing. All screw
holes are pre-drilled for reversing the door. A Phillips screwdriver is
required.
Warning The electronics drawer contains hazardous voltages. If the unit

has been in service, disconnect the power cord from both the unit and the
power source, turn off all gas regulators, disconnect all tubing and any
other connections from the rear of the electronics drawer, and allow the
unit to cool before reversing the door swing. s
1. Referring to Illustration A following, open the outer door and remove

it by lifting it off the hinge pins. Place the door on its face on a padded
surface to prevent scratches.
2. Remove the hinge pins and two outer door hinges identified in the
illustration.
3. Use the Phillips screwdriver to remove and save the four nylon screws
from the outer door hinge mounting holes on the right side of the
door frame.
4. Rotate the outer door hinges 180° and install them loosely, in the
locations where the nylon screws were just removed, opposite their
original positions.
5. Install the saved nylon screws in the previous locations of the outer
door hinges.
6. Remove and save the four nylon screws from what will be the new
locations for the inner door hinges.

Section 5
Routine Maintenance
Reversing the Door 7. Remove the electrical connector on the top of the inner door hinge by
carefully pulling up the black strain relief.
Swing (continued)
8. Refer to Figure 5-5. The heater
wiring connector is of yellow
rubber which should be visible
when the strain relief is moved
upward as shown. See connector
manufacturer’s instructions in
Section 11 of this manual.
9. Grasp the upper portion of the
yellow connector and pull it up
and out of the hinge socket.
Caution The frame along the hinge
side of the inner glass door has Figure 5-5. Connector
electrical connectors mounted on
the frame at both the top and bottom of the door. Be careful not to
damage the connectors by resting the weight of the door on them. Place
the inner glass door on a flat surface where it cannot be damaged. s
10. Release the inner door latch. While holding the inner door securely,
remove the upper door hinge. Lift the inner door carefully out of the
bottom hinge and set it aside.
11. The upper inner door hinge will be inverted and become the lower
inner door hinge when the hardware is reassembled. At this time,
remove the 5/8” black plastic bushing from the hinge. This bushing
will be inserted in the top hinge when the door is installed on the right
side of the cabinet.
12. Remove the lower inner door hinge. This hinge will be inverted and
become the upper inner door hinge when the hardware is reassembled.
Also, remove the 1” long white plastic bushing from the center of the
hinge and insert it into the hinge removed in step above.
13. The shoulder of the white bushing must be on top as shown in
Illustration B. Install the black plastic bushing into the hinge just
removed. See Figure 5-6.
14. Remove the two nylon screws opposite the door latch.
15. Remove the door strike plate and install the two nylon screws just
removed, into the vacant holes.
16. Remove the two outer Phillips screws on the frame just beneath the
control panel. Remove only the two outer screws and pull the
electronics drawer out about one inch.

Section 5
Routine Maintenance
Illustration A. Disassembly
Illustration B. Reassembly

Section 5
Routine Maintenance
Reversing the Door Swing Refer to Illustration B on the previous page.

(continued) 15. Move the door heater cable to the slot on the right side of the
electronics drawer.
16. Push any excess cable into the area under the electronics drawer to
avoid damaging the cable when drawer is pushed back in and secured.
18. Install the lower inner door hinge in the drawing. This was the upper
inner door hinge removed in Step 10 and should have the white plastic
bushing installed. Do not completely tighten the screws.
18. Insert the inner door into the lower hinge with the latch to the left.
Align the door to chamber opening and place upper hinge in position.
This was the lower inner door hinge removed in Step 12 with the black
plastic bushing installed.
19. Insert the screws into the hinge but do not completely tighten them.
20. Match the pins on the yellow connector with the socket on the door.
Press the connector completely into the socket
and slide the strain relief down until no yellow is Cable in slot
visible on the plug (Figure 5-6).
21. Slide the electronics drawer back in, being very
careful to place the door heater cable back into
the provided slot so that the drawer does not Black bushing
pinch the cable (Figure 5-6). Secure the Figure 5-6. Connector
electronics drawer with the screws removed above.
22. Align the silver power buss visible through the glass along the hinge side
of the door with the gasket along right side of chamber opening.
23. When both inner door hinges are in place with the hinge screws still
loosened, push up on the bottom hinge. This will shift both hinges and
the inner door upward. Tighten the hinge screws.
24. Attach the strike plate, aligning it so the knob secures the door against
the gasket.
25. Re-install the hinge pins.
26. Install the nylon screws in the previously upper and lower inner hinge
locations.
27. Mount the outer door onto the hinge pins. Close the door to verify
that the door is located properly. Open and hold the outer door in
place while tightening the hinge screws before closing the door.
28. Plug the power cord back into unit and the the dedicated, grounded
circuit. Turn power switch on. Allow the unit to run until the
temperature stabilizes. Check for proper unit operation, especially inner
door heater and gasket seal. Clean and disinfect the unit before
returning to service.

Section 5
Routine Maintenance
Replacing Fuses Warning The electronics drawer contains hazardous voltages. Replacing
the fuses should be performed by qualified personnel only. If the unit has
been in service, disconnect the power cord from both the unit and the
power source, turn off all gas regulators, disconnect all tubing and any
other connections from the rear of the electronics drawer. s
CAUTION
There are three fuses in the incubator that can be replaced. To replace a
fuse:
1. Open the exterior cabinet door.
2. Remove two screws as shown. See Figure 5-7.

Silence Mode Enter

Inject % CO2
Door Heater Cable Slot Locations

(left hand door swing shown)
Door Heater
Wiring & Connector
Remove these screws

to slide out electronics drawer
Figure 5-7. Screw Locations
3. Carefully slide out electronics drawer.
There are two fuses on the main microboard labeled F1 (for 24VAC Door
Heater) and F4 (for 24VAC Collar Heater). Refer to Figure 5-8 for the
location of the main microboard. Remove the fuse and replace it with a
new one of the same specification. Refer to Table 5-1.
Table 5-1. Fuses and Specifications
Fuse # Manufacturer Part # Amperage Rating Rupture Speed IEC Letter Code
F1 BUSS GMC-3.5A 3.5 Amp Time-Lag T
F4 BUSS GMC-2.5A 2.5 Amp Time-Lag T
115 VAC ACC BUSS GMC-1.0A 1.0 Amp Time-Lag T
230 VAC ACC BUSS BK-GMC-500mA 0.5 Amp Time-Lag T

Section 5
Routine Maintenance
Replacing Fuses The other replaceable fuse is the accessory outlet fuse mounted to the floor
of the electronics drawer. To locate the fuse, refer to Figure 5-8. Remove
(continued) the fuse and replace with a new one of the same specification.
1. When the fuse has been replaced, slide the electronics drawer back in,
being very careful to place the door heater cable back into the provided
slot (Figure 5-7) so that the drawer does not pinch the cable.
2. Replace the two screws removed earlier.
3. Close the exterior door.
4. Plug the power cord back into unit and dedicated, grounded circuit.
5. Turn on power switch. If the unit operates properly, it may now be

returned to service.
There is one fuse in the incubator that is not replaceable. This fuse is
intended for catastrophic failure only and is located on the power supply
circuit board in the electronics drawer. See Figure 5-8. If this fuse is
blown, the power supply must be replaced. Contact the factory for more
information.
O2 Option
Only
Power CO2 Solenoid O2 Solenoid

Supply (valve on bottom) (valve on top)
Fuse for
IR
Accessory Analog
Module
Outlet Board
O2 Module
Power Transformer
Export Only
Power
Transformer Microprocessor Board
Power Switch/
Circuit Breaker
Figure 5-8. Electronics Drawer

Section 5
Routine Maintenance
HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is displayed
by following the Installing HEPA Filter procedures of Section 1. Carefully
Maintenance pull down the HEPA filter to remove, and discard it per local EHS policies
and procedures. Afterwards, clear the visible alarm and display, and reset
the timer by following the NEW HEPA Filter procedures of Section 3.
The REPLACE HEPA reminder can be set to alarm after a specified time
from 1 to 12 months. The reminder default is the factory recommended
setting of 6 months. For details, see Section 3.
Replacing Air Sample The air sample filter should be replaced whenever the HEPA filter is
Filter replaced. On the inside of the chamber, inserted into the back of the
blower scroll, is the air sample filter and its connecting tubing. Disengage
the tubing from the back of the scroll, then remove the filter from the
tubing and discard per local EHS policies and procedures. Install the new
filter. Connect it securely to the air sample filter tubing, then insert the
tubing into the back of the blower scroll.
Replacing Access The access port filter should be replaced whenever the HEPA filter is
Port Filter replaced. The filter is connected to the stopper in the upper left corner of
the chamber back wall. Remove the filter from the connecting tube and
discard per local EHS policies and procedures. Install the new filter.
Draining Water Note Although the rust inhibitor is biodegradable, be certain to follow
Jacket local EHS policies with regards to personal protective equipment, cleaning,
and disposal.
Caution For stacked incubators, completely drain the top unit before
draining the bottom unit in order to maintain the lowest possible center of
gravity. s
1. Turn the unit off. Remove the plug from the power source.
2. Remove the front cover plate below the door. There are small flatblade
screwdriver pry slots on each end of the plate to help remove it. See
Figure 5-9.
Figure 5-9. Front Cover Plate Below the Door

Section 5
Routine Maintenance
Draining Water 3. If present, remove the drain plug.

Jacket (continued) 4. Connect the hose barb insert to the drain on lower front of the water
jacket and to the drain hose.
Figure 5-10. Water Jacket Drain and Hose Barb Insert
5. After water jacket has finished draining, Press to release

remove the hose barb insert and secure on
the front of the unit. See Figures 5-10
and 5-11. Figure 5-11. Hose Barb Insert
Warning After the jacket is empty, be certain to clean up any water that
may have spilled onto the floor during this process to prevent a possible
slip hazard.
6. Install cover plate.
7. To fill the water jacket, see Section 1. Be sure to add the rust inhibitor
to the water when filling. Instructions follow in this section.
Adding /Replenishing Model 3100 Series incubators are shipped from the factory with a rust
Rust Inhibitor inhibitor added to 0.2 gallons (0.8 liters) of water in the jacket. The rust
inhibitor must be replenished every 2 years. Mix 1 bag/bottle of the rust
inhibitor with 1 gallon (3.8 liters) of water (quality as described in Filling
Water Jacket, Section 1).
Test the liquid level switch by draining 1.5 gallons (5.7 liters) of water
from the jacket per Draining Water Jacket instructions above. If the
message center does not display ADD WATER, then the liquid level
switch could be faulty. Call Technical Services.
Add the rust inhibitor/water solution to the water jacket and continue to
fill the jacket per Filling Water Jacket, Section 1.
Rust Inhibitor (0.5 lb.) bag 1900100
Rust Inhibitor (800ml) bottle 1900101

(use in units with a cooling coil)

Section 5
Routine Maintenance
Replacing O2 Sensor Warning Handle the O2 sensor fuel cell very carefully as it can be easily
damaged. Do not install the O2 sensor fuel cell if its shipping box is
damaged or it has fallen on the floor as mechanical shock or vibration may
cause irreversible internal structural damage to the O2 sensor fuel cell. Do
not disassemble the O2 sensor fuel cell as there are no user-serviceable
adjustments. If the liquid electrolyte leaks due to sensor breakage, care
should be taken in handling the sensor as the solution contains lead acetate
which is harmful to humans.
Warning Contact with this liquid should be avoided. In case the liquid
electrolyte contacts the skin or clothing, wash with soapy water and rinse
generously with plain water. If the liquid electrolyte contacts the eye, flush
with water for at least 15 minutes and obtain immediate medical
assistance. In case of breathing in of the electrolyte, flush the nasal cavity
thoroughly with water and seek immediate medical assistance. If the
electrolyte is swallowed, rinse the mouth thoroughly with water and seek
immediate medical assistance.
The O2 sensor is located on the blower scroll plate in the chamber of the
unit. To replace it, see Figure 5-12 and 5-13 and follow the steps below.
Warning If the unit has been in service, disconnect the power cord from
both the unit and the power source, turn off all gas regulators and allow
the unit to cool before replacing the O2 sensor fuel cell.
1. Remove the top duct by removing 2 wingnuts.
2. Locate the sensor on the scroll plate.
Warning Before removing the old O2 sensor fuel cell, inspect it for any
visual signs of damage. If the liquid electrolyte is leaking, follow local
EHS policies with regards to personal protective
equipment, cleaning, and disposal.
Figure 5-12. Underside View of Top of Chamber

Figure 5-13. Detail

Section 5
Routine Maintenance
Replacing O2 Sensor 3. Lift up slightly on the tab securing the sensor wire terminal
(continued) connection. Be careful not to break the locking tab off. See enlarged
detail of Figure 5-13.
4. Disconnect the connectors from each other.
5. The O2 sensor fuel is screwed into the brass fitting. Unscrew the old
sensor and discard per local EHS policies and procedures.
Warning Before installing the new O2 sensor fuel cell, inspect it for any
visual signs of damage. If the liquid electrolyte is leaking, handle per local
EHS policies with regards to personal protective equipment, cleaning, and
disposal.
6. Screw the new O2 sensor fuel cell in firmly. Be careful not to cross-
thread.
7. Orient the connectors as shown in Figure 5-13 and connect.
8. Install the top duct and tighten the two wingnuts.
9. Plug the power cord into the unit and the dedicated, grounded circuit.
Turn the power switch on.
10. Calibrate the O2 system using the O2 [email protected]% method as

described in Section 2.
11. Allow the unit to run until the temperature stabilizes. Check the O2
and CO2 operation and return the unit to service.

Section 6 Factory Installed Options
Remote Alarm A set of relay contacts are provided to monitor alarms through an RJ-11
telephone style connector on the back of the cabinet. Refer to Figure 1-13
Contacts for the location of the alarm connector.
The remote alarm provides a NO (normally open) output, a NC (normally

closed) output and COM (common). Refer to Figure 6-1.
(NO) (NC)
ALARM CONTACTS
green wire red wire 30V max at 1A max
(COM) (not connected)
yellow wire black wire
(2) COM
(3) N.O. (4) N.C.
Alarm Contacts
Junction Box
phone cable to incubator

Stored product should be protected
alarm contacts RJ-11 connector by an activated alarm system capable
of initiating a timely response 24 hours/
All outputs shown day. These alarms provide interconnect
in alarm condition for centralized monitoring.
Figure 6-1. Contacts
The contacts will trip on a power outage or an over temperature condition.

The contacts may also be programmed to trip or not trip on temperature
alarms, CO2 alarms, O2 alarms, and RH alarm. See Section 3, System
Configuration.
Note After connecting the incubator to the external alarm system, verify
proper alarm operation by simply placing the incubator power switch to its
off position to simulate a power outage condition.

Section 6
Factory Installed Options
Connecting the RS485

Interface (190523) CAUTION
All incubator models can be purchased with the RS485 communications

option. This option allows the incubator to be directly connected to a
Model 1535 alarm system without the use of a communications module. A
junction box is provided with each RS485 option. Refer to Figure 6-2 for
wiring details.
Figure 6-2. Wiring
To allow the incubator and the 1535 to communicate, an address must be

allocated on the 1535. Refer to the 1535 operating manual. The same
address number must be assigned to the incubator. Refer to Section 3 of
this manual, System Configuration.
Note After connecting the Model 1535 Alarm System to the incubator,
verify that there are no RS485 communication errors per the Model 1535
operating manual. Simulate incubator failures of each module to verify
proper Model 1535 operation.

Section 6
Connecting Analog
Output Boards CAUTION
The analog output board option allows the incubator to output analog
signals representing the air temperature of the cabinet, the CO2 content ,
the O2 content, and the relative humidity, depending upon which systems
are in the incubator. There are three different analog output board options
available : 0-1V, 0-5V, or 4-20mA signals. Negative display readings will
output 0V. See Table 6-1 for output specifications of the three boards.
Table 6-1. Analog Output Board Specifications

190512 4-20mA 190544 0-1V 190543 0-5V
Output Scaling Output Scaling Output Scaling
4-20mA Equals 0-1V Equals 0-5V Equals
Temp 00-100°C 0.0-100°C 0.0-100°C
RH 0-100%RH 0-100%RH 0-100%RH
CO2 0.0-100.0%CO@ 0-100.0%CO2 0-100.0%CO@
O2 0.0-100.0%O2 0-100.0%O2 0-100.0%O2
For the 0-1V and 0-5V boards, the recording device must supply a load
>/=1000 ohm. For the 4-20mA board, the recorder must supply a load of
</=100 ohm. The outputs do not have isolated grounds.
To wire in the analog output board, a 22-gauge, 3-conductor shielded

cable (Part # 73041) is recommended, maximum length 50 ft (15.2m).
Warning The electronics drawer contains hazardous voltages. Opening

the drawer and/or wiring in an analog board should be performed by
qualified personnel only. If the unit has been in service, disconnect the
power cord from both the unit and the power source, turn off all gas
regulators, and disconnect all tubing and any other connections from the
rear of the electronics drawer. s

Section 6
Connecting Analog 1. Open the exterior cabinet door and remove the two screws shown in
Figure 6-3.
Output Boards (cont.) Silence Mode
Enter
Inject % CO2
Door Heater Cable Slot Locations

(left hand door swing shown)
Remove these screws

to slide out electronics drawer
Figure 6-3. Screw Locations
2. Carefully slide out electronics drawer and locate the Analog Output
board (Figure 6-4).
3. Each system monitored ( Temp, CO2, O2, RH) requires two
conductors. Feed the wire through the analog wiring inlet on the back
of the drawer. See Figure 6-5.
CO2 RH Analog Wiring
Temp O2 Inlet
+-+- +-+-
J2
J3
TEMP + _ + _ + _ + _
CO2 TEMP Co2 RH O2
RH
O2
COM
Figure 6-4. Analog Output Board Figure 6-5. Analog Wiring Inlet
4. Strip the ends of each conductor and wire to the appropriate terminals
of connectors J2 and J3 on the analog board.
5. When wiring is completed, slide the electronics drawer back in being
very careful to place the door heater cable back into the provided slot
so that the drawer does not pinch the cable. See Figure 6-3.
6. Replace the two screws removed earlier.
Note Accuracy of the analog outputs measured at the circuit board
terminal strip with respect to the incubator display is ±1 unit. There is no
calibration of the analog outputs by the incubator, so the instrument(s)
connected to the analog outputs must be calibrated to match the incubator
display before returning to service.

Section 6
Gas Guard for CO2 or Model 3100 Series incubators can be equipped with a built-in gas guard
system that will operate with either a CO2 or a N2 gas supply. Only one
N2 (190640/190642) gas guard can be installed on each unit. The gas guard uses two pressure
switches to continuously monitor the pressures of two independent CO2
or N2 supplies and automatically switches from one supply to the other
when the supply pressure drops below 10 psig (68.95 kPa). The gas guard
is not designed to be used with multiple incubators.
Both of the CO2 or the N2 gas supplies must be equipped with two-stage
pressure regulators. The high pressure gauge at the tank should have a 0-
2000 psig range and the low pressure gauge should have a 0-30 psig range.
The gas supply to the incubator must be maintained at 15 psig (103.4
kPa) maximum. Gas pressures below 15 psig (103.4 kPa) may cause
nuisance alarms to occur on incubators equipped with the built-in Gas
Guard.
Optional Exit for Optional Power Cord
a d c
RS 485 Analog Wiring N2 Inlet CO2 Inlet #1 Connector
c
Remote Air Makeup CO2 Inlet #2 Convenience
a b
Alarm Inlet Electrical Outlet
a RJ-11 telephone style connectors b Used with optional IR sensor
c Used with optional Gas Guard system d Used with optional O2 sensor
Figure 6-6. Unit Back with All Connections
Connecting CO2 Gas The CO2 inlets for a built-in gas guard are located on the rear of the
electronics drawer (Figure 6-6). Using 1/4” ID tubing, connect one of the
Supplies CO2 supply tanks to the fitting labeled CO2 Inlet #1. Connect the
second CO2 supply tank to the fitting labeled CO2 Inlet #2. Install 3/8”
hose clamps to secure the 1/4” ID tubing to the barbed fittings on the
regulator and the rear of the electronics drawer.

Section 6
Connecting N2 Gas Important When using Nitrogen (N2), the gas inlet labels on the back
Supplies panel will be different than the standard configuration shown in Figure 6-6
as follows; (from left to right) CO2 INLET, N2 INLET #2, and N2
INLET #1.
The N2 inlets for a built-in gas guard are located on the rear of the
electronics drawer (see Figure 6-6). Using 1/4” ID tubing, connect one of
the N2 supply tanks to the fitting labeled N2 Inlet #1. Connect the second
N2 supply tank to the fitting labeled N2 Inlet #2. Install 3/8” hose clamps
to secure the 1/4” ID tubing to the barbed fittings on the regulator and the
rear of the electronics drawer.
De-activating Built-in The built-in Gas Guard is turned ON when shipped from the factory. In
Gas Guard addition, the Tank Select for the gas guard is specified as Tank 1 when
shipped. Refer to Section 3, Configuration, to de-activate the Gas Guard
or change the Tank Select from 1 to 2. If the operator does not want to
use the Gas Guard, the incubator functions normally by supplying CO2
(or N2) through the CO2 Inlet #1 (or the N2 Inlet #1).
Operation of CO2 or N2 With the Gas Guard in operation, the incubator will use the gas supplied
through CO2 (or N2) Inlet #1 until the pressure drops below
Gas Guard approximately 10 psig. At this time, the Gas Guard automatically switches
to the gas supplied through CO2 (or N2) Inlet #2.
In addition, the incubator automatically changes the Tank Sel in

Configure mode from 1 to 2 to indicate that the incubator is now using
gas supplied through CO2 (or N2) Inlet # 2. If the gas supply to CO2 (or
N2) Inlet #1 is replenished, the incubator will continue to operate using
the gas supplied through CO2 (or N2) Inlet #2 unless the operator
changes the Tank Select from 2 to 1 through the Configuration mode.
Refer to Section 3, Configuration.
An audible alarm and two visual alarms occur on the control panel when
the gas guard switches from one supply to the other. The audible alarm
will sound until the operator presses the Silence key on the control panel.
A visual alarm in the Message Center will read Tank 1 Low while the
audible alarm is sounding, but will be removed after the operator presses
the Silence key.

Section 6
Operation of CO2 or N2 Neither of the audible and visual alarms described above ring back once
the Silence key is pressed. However, there is a second visual alarm, Tank
Gas Guard (cont.) Low, that illuminates in red below the Silence key on the control panel
when either of the two gas supplies fall below 10 psig (68.95 kPa). The
Tank Low remains illuminated as long as the gas guard detects a low
pressure on either of the gas supply lines. This message is a reminder for
the operator to replace or check for insufficient gas supply to the
incubator.
If the gas guard does not detect an adequate gas supply at the CO2 (or
N2) Inlet #1 or CO2 (or N2) Inlet #2, a visual and audible alarm will
again occur on the control panel. The visual alarm in the Message Center
will read Tank 1&2 Low. The audible alarm will continue to ring until
the Silence key is pressed. The audible alarm will ring back every 15
minutes after the alarm is silenced, if the Gas Guard continues to detect
that both gas supply pressures are below 10 psig (68.95 kPa).
Humidity Readout The 3100 Series incubators can be equipped with a humidity sensor to
monitor the relative humidity (RH) inside the chamber. The sensor is
(190643) mounted to the top air duct and provides a signal that is displayed in 1%
increments on the control panel. The humidity readout can be displayed
continuously or toggles with the temperature readout. In addition, a low
alarm limit can be set on the humidity readout which will detect when the
humidity pan runs dry. Refer to Section 3, Configuration.
Factors Affecting Humidity Level in Chamber:

• Water level in the humidity pan
• Frequency of door openings
• Humidity pan location; floor, shelf, in duct
• Flooded bottom of chamber
• Air leakage through the gaskets
• Gas sample port capped
• Standard HEPA filter or VOC HEPA2 filter or air flow restrictor plate
• N2 purge on incubators with O2 control.
• Humidity levels in O2 units (Models 3130/3131, 3140/3141) will be
reduced, depending on the amount of N2 required to control the O2
level in the chamber.

Section 6
Humidity Readout The following table lists some typical RH levels at different O2 and CO2
(190643) continued percentages.
Table 6-2. Typical RH levels

O2% CO2% RH% (±5%)
1% 2.5% 55%
2% 5% 60%
5% 10% 75%
10% 10% 80%
21% 5% 95%
Incubators equipped with a Thermal Conductivity CO2 sensor rely on a

constant level of relative humidity in order to accurately measure and
control the CO2 concentration in the incubator.
The humidity sensor is capable of measuring relative humidity from 10%

to 100% with an accuracy of ±5% above 90%. See Section 2, Calibration
for details on calibrating the humidity readout.
Cooling Coil Note For customer convenience, 12 ft. of 3/8” I.D. vinyl tubing with 4
clamps is included in the shipping materials. s
The operating (setpoint) temperature range of the incubator with the

cooling coil installed is from +5°C above ambient down to +15°C.
The cooling coil incubator incorporates a finned, U-shaped copper pipe

installed within the water jacket. This pipe routes chilled water supplied by
a laboratory bath. (Be aware that your bath may not be set to restart after a
power failure. Read the manufacturer’s operating instructions.)
Verify that the supply line pressure does not exceed 20 psig (138KPa).
When the cooling coil is in use, several factors affect the uniformity inside
the incubator chamber: the temperature difference between the operating
temperature set point and the bath water temperature; the flow rate of the
chilled water, and the on-time percentage of the door heaters.

Section 6
Cooling Coil As determined in carefully controlled laboratory tests, the smaller the
difference between the temperature of the bath and the setpoint
(continued) temperature of the incubator, the better the uniformity. However,
decreasing this temperature difference does cause less control of the system
because if the bath does not cool the water jacket adequately, the heaters
do not cycle and the chamber temperature simply drifts with the ambient
temperature of the room.
Tests have shown that as a starting point, operating the bath at 2°C to 3°C
below the incubator’s operating setpoint temperature, with a cooling water
flow rate of 1/2 to 1 GPM (gallons per minute), should result in good
control and uniformity.
Caution Because of the efficiency of the cooling coil design, it is possible

for condensation to occur on the outside of the incubator’s water jacket
when operating in certain ambient temperature and relative humidity
conditions. The condensation will then saturate the fiberglass insulation
between the water jacket and the incubator cabinet. s
Warning Clean up any condensation that may leak out onto the floor to
prevent a possible slip hazard. s
Using psychometric data from the Carrier® Psychometric Chart, curves of

maximum allowable RH versus ambient temperature can be plotted for
different incubator/bath conditions (see Figure 6-7).
Figure 6-7. RH Limits to Avoid Water Jacket Condensation
For example, if the bath is to be operated at 15°C and the ambient

temperature is about 28°C, the RH in the room must be less than 45% to
avoid condensation forming between the water jacket and the outside of
the cabinet.

Section 6
Installing Cooling Coil Hose barbs are factory installed into the fittings on the back of the cabinet,
Incubator as shown below. They can be used as the water inlet or outlet to the bath.
Locate the vinyl tubing and hose clamps shipped inside the incubator. Cut
the tubing in half. The bath can be located up to six feet from the
incubator.
Figure 6-8. Back of Unit

Section 7 Specifications
*Specifications are based on nominal voltages of 115V or 230V in ambients of 22°C
to 25°C.
Temperature
Control ±0.1°C
Range* +5C above ambient to +55C (models 3110, 3111)
+5C above ambient to +50C (models 3120, 3121)
+5C above ambient to +45C (models 3130, 3131, 3140, 3141)
Uniformity ±0.2°C @ +37°C
Sensor Precision thermistor
Tracking Alarm User programmable high/low
*Cooling coil option extends low end temperature range to 15°C
Over Temperature Protection

Sensor Precision thermistor
Controller Independent circuit
Setability 0.1°C
Range Temp set point +0.5°C to 60°C
Tracking Alarm Not user programmable
CO2/O2
CO2/O2 Control Better than ±0.1%
CO2 Range 0-20%
O2 Range 1-21%
Inlet Pressure 15 PSIG (103.4 kPa), maximum
CO2 Sensor T/C or IR
O2 Sensor Fuel Cell
Readability 0.1%
Setability 0.1%
Tracking Alarm User programmable, high/low
Humidity
RH Ambient to 95% @ +37°C (98.6°F)
Humidity Pan 0.8 gal. (3 liters) standard
Tracking Alarm User programmable, low only
Optional Display 1% increments

Section 7
Specifications
Fittings
Fill Port 3/8” barbed
Drain Port 3/8” barbed, quick-connect
Access Port 1¼” (3.18cm) removable neoprene plug
CO2/N2 Inlets 1/4” hose barbed
Unit Heat Load (typical @ +37°C)

115V/230V 344 BTUH (100 Watt)
HEPA Filter
Federal Standard 209E, Class 100 - maximum allowable number of particles
0.5 microns and larger per cubic foot of air *
full recovery within 5 minutes of door opening
Replacement Reminder User programmable
Optional Filtration System Volatile Organic Chemicals (VOC)
*follow manufacturer's recommendations for regular filter replacement
Shelves
Dimensions 18.5” x 18.5” (47.0cm x 47.0cm)
Construction Stainless steel, perforated, electropolished
Surface area 2.4 sq. ft. (0.22 sq. m) per shelf
Max. per Chamber 38.4 sq.ft. (3.6 sq. m)
Loading 35 lbs (16kg) slide in and out, 50 lbs (23kg) stationary
Standard # 4 (shipped with unit)
Maximum # 16
Construction
Water Jacket Volume 11.7 gal. (44.3 liters) typical
13.0 gal. (49.2 liters) maximum
Interior Volume 6.5 cu. ft. (184.1 liters)
Interior Type 304, mirror finish, stainless steel
Exterior 18 gauge, cold rolled steel, powder coated
Outer Door Gasket Four-sided, molded magnetic vinyl
Inner Door Gasket Removable, cleanable, feather-edged, silicone
Electrical
115V Models 115VAC, 50/60 Hz, 1 PH, 3.6 FLA
230V Models 230VAC, 50/60 Hz, I PH, 2.0 FLA
Circuit Breaker/Power Switch 6 Amp/2 Pole
Convenience Receptacle 75 Watts max. (one per chamber)

Section 7
Specifications
Electrical (continued)
Remote Alarm Contacts Power interruption, over temperature,
deviation of temp., CO2, O2, and RH, customer
connections through jack on back of unit. 30V,
1A max.
Optional Data Outputs RS-485, 0-1V, 0-5V, 4-20mA
Dimensions
Interior 21.3” W x 26.8” H x 20.0” F-B, (54.1cm x 68.1cm x 50.8cm)
Exterior 26.3” W x 39.5” H x 25.0” F-B, (66.8cm x 100.3cm x 63.5cm)
Weight (per unit)

Net 265 lb. (120.2 kg) before filling water jacket
Net Operational 365 lb. (165.6 kg) after filling water jacket
Shipping 324 lb. (147.0 kg)
Certifications
Thermo Fisher Scientific declares that the applicable CE marked models
meet the provisions of the following EC directives.
204/108/EC Electromagnetic Compatibility Directive
2006/95/EC Low Voltage Directive
2011/65/EU RoHS Directive
CE Declarations are available upon request from the factory.
Safety Specifications
Indoor Use Only
Altitude Up to 2000 meters
Temperature 5°C to 40°C
Humidity Maximum 80% RH for temperatures up to 31°C,
decreasing linearly to 50% RH at 40°C, non-condensing
Mains Supply Fluctuations Not to exceed ±10% of the nominal voltage
Installation Category 21
Pollution Degree 22
Class of Equipment 1
1 Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand
safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II which is the
category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research
laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V
supply.
2 Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes
that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation.

Section 8 Spare Parts
All Models Part # Description
360171 Liquid Level Switch
191534 Feather Gasket
113002 5/16-18 Glide Foot
132046 115/230V Dual Heater
132049 Face Heater 40W, 24VAC
1900203 Heated Inner Door
190619 Left Inner Door Hinge
190618 Right Inner Door Hinge
700013 0.500” Flanged Nylon Bearing
990026 Door Gasket w/ Magnet
290184 Temp Probe 2252 Ohm
192057 Micro Board (for Release 7 units)
400202 12VDC Single Output Power Supply (Release 7 units)
190609 Display Board Assembly
230153 6A Circuit Breaker/Switch
460157 Line Filter/Power Inlet
420096 130VA Transformer, 12/24V
230159 3.5 Amp Fuse - Microboard
230158 2.5 Amp Fuse - Microboard
250087 Solenoid Valve Kit w/assorted fittings
1900071 Blower Motor Replacement Kit
100113 Blower Wheel 3.5x1.5 CCW
190846 Blower Scroll Assembly
103072 Blower Plate Gasket
1900601 Service Kit (P/N 290090 T/C type CO2 sensor assembly)
103074 CO2 Sensor Plate Gasket
130097 #6 Silicone Stopper w/ 3/8” Hole
180001 Polypropylene Funnel
117044 Fill Port Cap
2270107 Micro Board/Power Supply Kit (to replace Micro Board
P/N 191634, Release 5 & 6 units)

Section 8
Spare Parts
All Models (continued) Part # Description

770001 Bacterial Air Filter (CO2 line, gas guard, sample, access port)
760210 10 Bacterial Air Filters (770001)
760175 HEPA Filter
760209 HEPA Value Pack of 4 Filters (760175)
760200 HEPA2 VOC Filter
760199 HEPA2 VOC Filter Accessory Kit
(760200 HEPA2, 117036 silicone plug, 101018 O-ring)
1900067 Filter Replacement Kit
([2] 770001 filter and 760175 HEPA)
1900094 HEPA2 VOC Filter Replacement Kit
(760200 HEPA2, 190985 access port filter assembly,
770001 filter)
1900123 Air Flow Restrictor Plate
190651 Decontamination Kit
190566 Inner Door Heater Cable
400201 40W Switcher Kit (replaces triple output power supply
P/N 400119, for units prior to Release 7)
1900548 Exterior Door Assembly SRO
Spare Parts - 115V 460024 115V Convenience Outlet

(3110, 3120, 3130, 3140) 230135 1.0A Fuse for Convenience Outlet
430108 Line Cord (North America 125V/15A)
Spare Parts - 230V 420097 43VA Transformer

(3111, 3121, 3131, 3141) 460138 Power Outlet, Snap-In Receptacle
230120 1/2 AMP Fuse for 230 V outlet
430109 Line Cord (Continental Europe)
430111 Line Cord (Denmark)
430112 Line Cord (Italy)
430113 Line Cord (Switzerland)
430114 Line Cord (United Kingdom)
430115 Line Cord (India)
430116 Line Cord (Israel)
430118 Line Cord (Australia)
430367 Line Cord (China)

Section 8
Spare Parts
Spare Parts - IR or O2 190885 IR Sensor

(3120/3121, 3130/3131, 191646 O2 Circuit Board
3140/3141) 250119 AC Solenoid
770001 Bacterial Air Filter (CO2 line, gas guard, sample and
access port)
250118 Valve, Sol, O2, 12VDC, 4W
290083 O2 Sensor Fuel Cell
190661 IR Sensor Filter
110065 Sample Port Cap
Spare Parts - Gas Guard 250121 Solenoid Valve, 3-way, 12VDC

(190640/190642) 360213 Pressure Switch
Spare Parts - Humidity 290154 RH Sensor Assembly, 1 ft.

Readout (190643)
Spare Parts - Data Output 190512 Analog Output Board 4-20 mA

Options 190543 Analog Output Board 0-5V
190544 Analog Output Board 0-1V
73041 22 Gauge, 3 Conductor, Shielded Cable
190523 RS485 Output Kit
Field Installation Kits for Gas Guard Kit, CO2 & N2 1900061
qualified service personnel Humidity Sensor Kit 1900062
RS-485 Output Kit 1900043
Analog Output* Kit, 4 - 20mA 1900044
Analog Output* Kit, 0 - 5Vdc 1900045
Analog Output* Kit, 0 - 1Vdc 1900046
* select only one type of optional analog output

Section 8
Section title

Section 8
Spare Parts

Section 8
Spare Parts

Section 8
Spare Parts

Section 8
Spare Parts

Section 9
Electrical Schematics

Section 9

Section 9

Section 9

Section 9

Section 9

Thermo Scientific
THERMO FISHER SCIENTIFIC WATER JACKETED INCUBATOR WARRANTY USA*
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time so the warranty will go into
effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year war-
ranty period.
During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense,
including labor. In addition, after the initial warranty period of one year, the CO2 sensor (IR) will be warranted for a second year, parts only. The CO2
sensor (T/C) will be warranted for four additional years for a total of five years, parts only. The water jacket chamber has a unit production lifetime war-

ranty, parts only, against rust-through and leaks. Unit production life is defined as the period of time the current chamber design is in production, plus
seven years. If corrosive chemicals are identified in the chamber, the warranty will be voided. Installation and calibration is not covered by this warranty
agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any repairs.
Expendable items, glass, filters and gaskets are excluded from this warranty.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component
part beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or equipment. At
Thermo's option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO WARRANTIES
OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential
damages including, without limitation, damages relating to lost profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manu-
als carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at l-800-438-4851 (USA and Canada) or 1-740-373-4763. We're ready to
answer your questions on equipment warranty, operation, maintenance, service and special applications. Outside the USA, contact your local distributor
for warranty information.
ISO
9001
Rev. 6 4/09 REGISTERED
Water Jacketed CO2 Incubator

10-1
10-2
THERMO FISHER SCIENTIFIC INTERNATIONAL WATER JACKETED INCUBATOR WARRANTY*
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows shipping time so the warranty will go into
effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the first year warranty
period.
Water Jacketed CO2 Incubator

During the first year, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense, excepting
labor. In addition, after the initial warranty period of one year, the CO2 sensor (IR) will be warranted for a second year, parts only. The CO2 sensor (T/C) will

be warranted for four additional years for a total of five years, parts only. The water jacket chamber has a unit production lifetime warranty, parts only,
against rust-through or leaks. Unit production life is defined as the period of time the current chamber design is in production, plus seven years. If corrosive
chemicals are identified in the chamber, the warranty will be voided. Installation and calibration is not covered by this warranty agreement. The Technical
Services Department must be contacted for warranty determination and direction prior to performance of any repairs. Expendable items, glass, filters and
gaskets are excluded from this warranty.
Replacement or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part
beyond the original warranty period. The Technical Services Department must give prior approval for return of any components or equipment. At Thermo's
option, all non-conforming parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL OR IMPLIED. NO WARRANTIES OF
MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential dam-
ages including, without limitation, damages relating to lost profits or loss of products.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals
carefully detail equipment installation, operation and preventive maintenance.
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or 1-740-373-4763. We're ready to
answer your questions on equipment warranty, operation, maintenance, service and special application. Outside the USA, contact your local distributor for
warranty information.
ISO
9001
Rev. 6 4/09 REGISTERED
Thermo Scientific
TURCK
Industrial Connectivity Products
picofast ® "Snap Lock" Connector Instructions
UNLOCK
With TURCK’s patented Locking Sleeve pulled back,

any picofast sensor slides on without any difficulty.
LOCK
After sensor is connected, push Locking Sleeve forward to

create a watertight connection. It’s a snap!
Installation Instructions
To Attach: To Detach:
1. Be sure black locking

1. Do not twist.
sleeve is pulled back.
2. Line up pins and push

connector onto plug. 2. Pull locking sleeve back
You will feel a “snap.” to “unlocked” position.
Do not twist.
3. Push locking sleeve forward

on connector until it is 3. Pull connector
flush with the front of the straight off.
connector.
4. If sleeve is difficult to slide

on a new connector, 4. Leave locking sleeve in
“exercise” it a few times. unlocked position.
Do not use tools.
Clearwater Tech - Phone: 800.894.0412 - Fax: 208.368.0415 - Web: www.clrwtr.com -

thermoscientific.com
© 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its
subsidiaries. Specifications, terms
and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative
for details.
Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road
Marietta, Ohio 45750
United States
A Thermo Fisher Scientific Brand

Manual INCUBADORA THERMO 3110 PDF

Uploaded by

Copyright:

Available Formats

Manual INCUBADORA THERMO 3110 PDF

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Manual INCUBADORA THERMO 3110 PDF

Uploaded by

Copyright:

Available Formats

User Manual

Model 3100 Series

Visit us online to register your warranty

Downloaded from www.Manualslib.com manuals search engine

Table 1. Single Chamber Models

Model CO2 Sensor* O2 Voltage ** Temp Max

3110 T/C No 115 55C

3111 T/C No 230 55C

3120 IR No 115 50C

3121 IR No 230 50C

3130 T/C Yes 115 45C

3131 T/C Yes 230 45C

3140 IR Yes 115 45C

3141 IR Yes 230 45C

*T/C is a thermal conductivity sensor.

MANUAL NUMBER 7053110

Thermo Scientific Water Jacketed CO2 Incubator i

Downloaded from www.Manualslib.com manuals search engine

Non- intended use:

©2014 Thermo Fisher Scientific. All rights reserved.

ii Water Jacketed CO2 Incubator Thermo Scientific

Downloaded from www.Manualslib.com manuals search engine

4 Always dissipate extreme cold or heat and wear protective clothing.

4 Always follow good hygiene practices.

4 Each individual is responsible for his or her own safety.

Thermo Scientific Water Jacketed CO2 Incubator iii

Downloaded from www.Manualslib.com manuals search engine

Do You Need Information or Assistance on

Thermo Fisher Scientific (Asheville) LLC

International customers, please contact your local Thermo Scientific distributor.

iv Water Jacketed CO2 Incubator Thermo Scientific

Downloaded from www.Manualslib.com manuals search engine

Repairs NOT Covered Under Warranty

Thermo Scientific Water Jacketed CO2 Incubator v

Downloaded from www.Manualslib.com manuals search engine

Section 2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Thermo Scientific Water Jacketed CO2 Incubator vi

Downloaded from www.Manualslib.com manuals search engine

Section 3 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Section 4 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Section 5 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

vii Water Jacketed CO2 Incubator Thermo Scientific

Downloaded from www.Manualslib.com manuals search engine

Section 6 Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Section 7 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Section 8 Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Section 9 Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Section 10 Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Section 11 Interior Door Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1

Thermo Scientific Water Jacketed CO2 Incubator viii

Downloaded from www.Manualslib.com manuals search engine

Figure 1-1. Water Jacket Incubator

• Outer Door - Reversible to opposite swing (Section 5).

• Heated Inner Door - Keeps chamber interior dry. Reversible to

• Main Power Switch

• Control Panel - Keypad, Displays and Indicators (Figure 1-2).

• Fill Port - Used for filling the water jacket.

• Leveling Legs - Used to level the unit.

• Water Jacket Drain – Use hose barb insert included.