Liasys Service Manual Rev. 08

LIASYS
Service Manual
Via E. Barsanti 17/A

00012 Guidonia – ROME (ITALY)
www.ams-analyzers.com
CONFIGURATION SHEET
CHAPTER DESCRIPTION REV.
INDEX TABLE OF CONTENTS 07
01 INTRODUCTION 07
02 SYSTEM DESCRIPTION 07
03 INSTALLATION 07
04 ELECTRICAL SCHEMES AND DRAWINGS 07
05 DIAGNOSTIC PROGRAM 07
06 SETTINGS AND ADJUSTMENTS 07
07 MAINTENANCE 07
08 HOST COMMUNICATION 08
09 ERROR SIGNALING AND TROUBLESHOOTING 07
10 ISE MODULE 07
Liasys Service Manual Rev. 08 01 October 2009 Page 1

GENERAL INDEX
TABLE OF CONTENTS
• Chapter 01 INTRODUCTION
• Chapter 02 SYSTEM DESCRIPTION
• Chapter 03 INSTALLATION
• Chapter 04 ELECTRICAL SCHEMES AND DRAWINGS
• Chapter 05 DIAGNOSTIC PROGRAM
• Chapter 06 SETTINGS AND ADJUSTMENTS
• Chapter 07 MAINTENANCE
• Chapter 08 HOST COMMUNICATION
• Chapter 09 ERROR SIGNALING AND TROUBLESHOOTING
• Chapter 10 ISE MODULE
Liasys Service Manual Rev. 07 01 June 2009 P age 1

Chapter 01 – INTRODUCTION
CHAPTER 01
- INTRODUCTION -
INDEX
1 INTRODUCTION ................................................................................................................. 1
1.1 THE AIM OF THE TECHNICAL MANUAL ……………………………………………..…..2
1.1.2 MANUAL OUTLINE……………………………..……………………..……………..………..2
1.2 SYSTEM INTRODUCTION ................................................................................................ 3
1.3 PRECAUTIONARY MEASURES ....................................................................................... 3
1.3.1 CHEMICAL RISKS ................................................................................................... 5
1.3.2 ELECTRICAL RISKS................................................................................................ 5
1.3.3 MECHANICAL RISKS.............................................................................................. 4
1.4 GUARANTEE ....................................................................................................................... 5
1.5 TECHNICAL OPERATING FEATURES………………………………………….…...... 6
1.5.1 COMPUTER & SOFTWARE FEATURES ............................................................. .9
1.5.2 OPTIONS.................................................................................................................... 8
1.5.3 DIMENSIOS, WEIGHT & ENVIRONMENT......................................................... 10
1.5.4 INSTALLATION REQUIREMENTS..................................................................... 10
Liasys Service Manual Rev. 07 01 June 2009 Page 1

1 INTRODUCTION
1.1 THE AIM OF THE TECHNICAL MANUAL
This manual has been written in order to supply the technical staff, the persons who are responsible
for the maintenance and for resolving instrument failures, a complete and detailed guide of the
Liasys analyzer, in accordanance with the standard UNI EN591 (which requires a manual to be
supplied with vitro diagnostic instruments for professional use).
1.1.2 MANUAL OUTLINE
The technical manual is composed of 10 chapters that not only describe the operative technical
characteristics of the system, but also the reparation and maintenance procedures to use for the
modules that they are composed of.
. Chapter 1 INTRODUCTION
The manual structure is described, and recommendations are given regarding the general
use of the analyzer. The technical characteristics of the operative system are given in this
chapter.
. Chapter 2 DESCRIPTION OF THE SYSTEM
Describes the system, in particular, the analytical cycle of the single modules and
electronic boards.
. Chapter 3 INSTALLATION
Describes the unpacking procedures and the required characteristics for the place of
installation.
. Chapter 4 ELECTRONICS SCHEMES AND DESIGNS
Gives the electronics schemes and assembly designs of the electronics board and the
assembly designs of the modules that the system is composed of.
. Chapter 5 DIAGNOSTIC PROGRAM
Describes the folders in the Diagnostic Program that are responsible for the checks and the
calibration of the modules in the system.

. Chapter 6 SETTINGS AND ADJUSTMENTS
Gives the procedures for substitution, adjustment and check of the system modules.
. Chapter 7 MANTENANCE
Describes the routine maintenance that needs to be done on a regular basis in order to
guarantee the correct functioning of the analyzer.
. Chapter 8 COMUNICATION WITH HOST
Describes the communication parameters and the ASTM protocol specifics used by the
Liasys system when connecting to the Host Computer.
. Chapter 9 ERROR SIGNALS AND GUIDE TO RESOLVE ANOMOLIES
Lists the system’s error signals, describes the errors in the results and gives a guide for how
to resolve anomolies.
. Chapter 10 MODULE ISE
This chapter contains information pertaining to the Module ISE, the product description,
characteristics and the technical specifics. Furthermore, it describes the maintenance
program and gives a guide for how to find failures.
The technical staff responsible for resolving the structural anomolies is highly suggested to
thoroughly examine the contents of this manual before operating the system.

1.2 SYSTEM INTRODUCTION
The “Liasys” system is a continuous-load, random access, bench top instrument for performing
chemical and immuno-turbo-dymetric clinical analysis. It is totally automatic and computer
controlled.
Via E. Barsanti 17/A

00012 Guidonia – ROME (ITALY)

1.3 PRECAUTIONARY MEASURES
1.3.1 Chemical risks
The individual operator is responsible for assuring that all possible precautionary measures are
taken against eventual risks associated with the use of the “Liasys” instrument in clinical laboratory
settings. The manufacturer will provide the reagents kit and specific written information on the use
of each of the reagents.
It is important that the samples be well coagulated and then carefully centrifuged.
Samples which contain fibrinogen clots can obstruct the probe and lead to inexact sampling.
If blood samples containing gel are used, it is suggested that the manufacturer’s recommendations
be followed.
Immediately clean and remove any accidental leakage of reagent or other liquid.
1.3.2 Electrical risks

As with any electrical device, the risk of electric shock exist.
Is therefore necessary to take every precautionary measure possible when working with this, or any
other, electrical instrument to avoid contact with power supply wires, electrical components or
electronic boards.
1.3.3 Mechanical risks

Several precautionary measures should be taken when operating the analyzer:
avoid wearing very loose clothing or jewelry that could become tangled in the instrument’s moving
parts (e.g. the sample probe); whenever possible, operate the instrument with the main cover panel
lowered.
WARNING: Never attempt to service or substitute any part(s) of the analyzer when the
instrument is turned on.
Any and all technical repairs or servicing must be performed by specialized personnel only.

1.4 GUARANTEE
AMS guarantees the substitution of all defective components and/or materials for a period of time
not above of 14 months starting from the date of invoicing. Saying guarantee, as well as Technical
Assistance, generally is intended furnished as net ex factory Rome.
This guarantee does not include consumable and instrument parts in contact with liquids. All
components not included in the guarantee are reported in the following table.
Besides guarantee does not cover damage caused by :

- improper use of the LIASYS instrument (or however not according to the Producer or Seller
instructions)
- bad transport
- insufficient (or missing) preventive maintenance by the User
In particular any damages due to the transport must be immediately reported to the carrier when he
delivers.
CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF GUARANTEE
Description Type Quantity Code
Reagents bottle 40 ml 30 pieces 9-65-0034-00

Reagents bottle 10 ml 25 " AS-650121
Glass reagent bottle 7 ml 10 " AS-900020
Reagents bottle 2 ml 25 " AS-650122
Conical sample cups 2 ml 1000 " AS-65-0001
Short sample cups 1 ml 1000 " AS-65-0100
Washing solution bottle 2 lt 1 " 9-35-0041-00
Kit tubing peristaltic pump 2 " 9-65-0040-00
Reaction cuvettes 60 " 9-65-0031-00
Drying Pad 1 " 9-01-0038-00
Halogen Lamp 1 " 9-35-0016-00
Tubes Kit – complete 1 " 9-65-0027-01
Tygon tubing 1 mt. 1 " 90-01253-00
Kit for E.V.Diluter connection 1 " 99-00906-00
Probe 1 " 9-05-0064-00

Probe new coating 1 " 9-05-0064-01

Micro-Pump Assembly 1 " 9-10-0028-01
Complete Probe Assembly 1 " 9-10-0062-00
Comp Probe Assembly New C 1 " 9-10-0062-01
Diluter head Teflon Fitting 10 " C1-01-0042-01
Diluter fitting 10 " C1-01-01222-00
E.V. Rinse fitting 10 " C190-01254-00
Solenoid Valve 1 " F-35-0019-00
Micro-Pump (up4) Assy 1 " 05-00403-00
Manifold Assembly 1 " 05-00405-00
Inlet/Outlet fitting for rinse… 1 " 01-01224-00
Lamps kit 5 " 9-65-0035-00
Na electrode 1 " 35-00814-00
K electrode 1 " 35-00815-00
Cl electrode 1 " 35-00816-00
Reference electrode 1 " 35-00817-00
Peristaltic Pump Tubing-head 1 " 35-00830-00
ISE inlet tubing connection 6 mt 1 " 35-00831-00
O-ring 4 " 35-00832-00

1.5 TECHNICAL OPERATING FEATURES
♦ Fully automatic, random access, continuous loading,

DESCRIPTION benchtop analyzer for clinical chemistry and
immunoturbidimetric assays
ASSAY TYPE ♦ End Point, Initial Rate, Kinetic, Bichromatic, Differential,
TEST ENTRY MODE ♦ Selective, Batch, Profiles, STAT
THROUGHPUT ♦ 200 test per hour without ISE module.
WORKING TEMPERATURE ♦ 37° C

♦ 4 removable racks (two racks cooled by Peltier cells
ON LINE REAGENTS and two racks at room temperature).
• 33+2 reagent positions of 40 ml or 7 ml each
(15 positions at room temperature and 20
cooled)
• 14 positions for Standards and Controls
♦ 5 racks for continuous loading of samples split in:
SAMPLE LOADING
• 4 racks for 16 positions each.
• 1 for 14 positions (STAT)
♦ Continuous loading
♦ Positive barcode reader
REACTION VOLUME ♦ Minimum 300 µl
♦ Maximum 670 µl
♦ A single mechanical arm provides all sampling
SAMPLING ARM operations with:
• Capacitive liquid level sensing
• Reagent pre-warming at 37° C
• Automatic probe washing
♦ Integrated module without syringe with the following
DILUTER specifications:
• Sample volume: 3.0 µl ÷ 99 µl (1 µl incr.)
• Reagent 1 volume: 3.0 µl ÷500 µl (1 µl incr.)
PRECISION ♦ CV < 1 % at 3 µl

READING SYSTEM ♦ Direct Photometry

♦ Photometer: interferential double channel filter
OPTIC SYSTEM wheel
♦ Wavelength: 8 narrow band interferential filters
from 340 nm to 620 nm
♦ Light source: halogen lamp 6V/10 W
♦ Linear range: 0.001 ÷ 2.500 Abs
♦ Resolution: 0.0001 Abs
OPTICAL PATH ♦ 10 mm.
♦ Composed of five probes that empty, wash and
WASHING STATION
dry the reaction cuvettes.
♦ 60 semi-disposable cuvettes
REACTION PLATE
♦ Cuvettes Q.C. continuously computer controlled
♦ Incubation temperature: 37°C
1.5.1. Computer & Software feature

TYPE ♦ IBM Compatible
CPU ♦ Pentium III 500 MHz, 512 Kb Cache or plus
♦ RAM 128 Mb or plus
MEMORY ♦ Hard Disk 10 Gb or plus
♦ Floppy Disk 3 1/2” 1.44 Mb
♦ Colour SVGA 15’’ low radiation
MONITOR Resolution 800 x 600 pixels;
max number of colors 65536 (16 bit)
♦ 80 Columns impact graphic (EPSON LX 300)
PRINTER
♦ PS2
♦ Two Bi-directional RS 232C serial ports and one
INTERFACE
parallel
♦ Multitasking WINDOWS 98 II E
SOFTWARE ♦ Italian, English, Chinese, Czech. Software to be released
AVAILABLE LANGUAGES soon in these languages: Russian, Portuguese, French,
Polish. Upon request it is possible to release the software
in other languages.
SETTINGS ♦ Disable all the energy saving options
♦ Disable the screen saver
♦ Select English “USA” as language
♦ Select date and time in Regional setting

NOTE: Even though the computers demonstrate the same technical and operative characteristics,
some of these could have different hardware installed.
This could cause problems for the Liasys software when running tests (A message appears
indicating “Random” error or blocks the program).
Therefore, if the PC is bought separately/locally, it is highly recommended to test the system at
your offices before preceding with the installation at the final client’s.
Consequently, AMS denies any responsibility for software problems that are due to buying the
computer separately from the instrument.
1.5.2 Options
♦ ISE MODULE
♦ POSITIVE BARCODE READER
1.5.3 Dimensions, Weight & Environment

♦ Height: 42 cm
DIMENSION ♦ Depth: 65 cm
♦ Length: 100 cm
WEIGHT ♦ 65 Kg
OPERATING ENVIRONMENT ♦ Temperature: 18°C ÷ 30°C.
♦ Relative humidity: 20% ÷ 85%
1.5.4 Installation requirements

POWER REQUIREMENTS ♦ Input Voltage 90 ÷ 250 Vac
♦ Input Frequency: 47 ÷ 63 Hz
♦ Power consumption:
♦ 300 W for the analytical unit
♦ 400 W for the work station
SAFETY REGULATIONS ♦ EN 61010-1:1993 +A2:1995
(in compliance with the main European safety
directives 73/23/CEE e 3/68/CEE)

ELECTROMAGNETIC ♦ EMC 89/336/CEE – 92/31/CEE Directives

COMPATIBILITY
♦ EN 55011, Class B, Group 1
♦ EN 50081-1:1992 EMC
♦ EN 55022
♦ ENV 50140 – ENV 50141
♦ EN 60601-1-2
♦ EN 61000-4
Warning: A steady power supply (+ 10%) must be provided for the instrument.
If it is not, the manufacturer highly recommends the use of:
♦ UPS Uninterruptible Power Supply ( No-break module)

♦ ELECTRONIC STABILISER

Chapter 02 – SYSTEM DESCRIPTION
CHAPTER 02
– SYSTEM DESCRIPTION -
INDEX
2 DESCRIPTION OF THE SYSTEM.................................................................................................... 3

2.1 ANALYSES CYCLE ............................................................................................................................ 4
2.1.1 REACTION PLATE ........................................................................................................... 4
2.1.2 REACTION PLATE CYCLE ............................................................................................ 4
2.2 SAMPLING ARM - OPERATIONAL SEQUENCE ....................................................................... 5
2.3 WASH STATION .................................................................................................................................. 7
2.3.1 WASH STATION CYCLE ................................................................................................ 7
2.4 ELECTRICAL SCHEMES DESCRIPTION ..................................................................................... 8
2.4.1ANALYTICAL CONTROL BOARD (CPU) P/N 00195-01 ......................................... 8
2.4.2 INTRODUCTION ............................................................................................................... 8
2.4.3 GENERAL CHARACTERISTICS ................................................................................... 8
2.4.4 CIRCUIT DESCRIPTION ................................................................................................. 8
2.4.5 POWER SUPPLY ............................................................................................................... 8
2.4.6 MICRO-CONTROLLERS AND BUFFER ..................................................................... 9
2.5 PROGRAMMABLE LOGIC ............................................................................................................. 14
2.5.1 FLASH PROGRAMMING VOLTAGE ......................................................................... 15
2.5.2 SERIAL INTERFACES .................................................................................................... 15
2.5.3 CLOCK ............................................................................................................................... 16
2.5.4 LED ..................................................................................................................................... 17
2.5.5 BUZZER ............................................................................................................................. 17
2.6 STEPPER MOTORS DRIVER BOARD P/N 00188-01 ............................................................... 17
2.6.1 INTRODUCTION ............................................................................................................. 17
2.6.2 GENERAL CHARACTERISTICS ................................................................................. 17
2.6.3 CIRCUIT DESCRIPTION ............................................................................................... 17
2.6.4 POWER SUPPLY ............................................................................................................. 17
2.6.5 STEPPER MOTORS DRIVER ....................................................................................... 18
2.6.6 OPEN COLLECTOR DRIVERS .................................................................................... 19

2.7 TA 8435 H ..19

2.7.1 INTRODUCTION ................................................................................................................ 19
2.7.2 MICRO-STEP PILOTING .................................................................................................. 19
2.7.3 CONTROL OF PULSE WIDTH MODULATION AND OUTPUT CURRENT ....... 20
2.7.4 CONTROL LOGIC .............................................................................................................. 21
2.7.5 1/2 STEP PILOTING .......................................................................................................... 21
2.8 SAMPLING INTERFACE ASSY P/N 00192-01 .......................................................................... 21
2.8.1 SAMPLING INTERFACE ASSY P/N 00192-01 .......................................................... 22
2.9 LEVEL SENSOR BOARD 1-2 P/N 30-0005-XX ..................................................................... 23
2.10 REACTION TRAY INTERFACE P/N 00193-01 ...................................................................... 23
2.11 REACTION TRAY INTERFACE P/N 00193-01 ...................................................................... 24
2.12 REACTION CHAMBER MOTOR INTERFACE P/N 00341-00 ............................................ 25
2.13 PRE-AMPL/ADC P/N 00107-00.................................................................................................... 25
2.14 PHOTOMETER LAMP PWS P/N 30-0004-00 ......................................................................... 25
2.15 SAMPLE RACKS IDENTIFICATION BOARD P/N 00446-00 ............................................. 26
2.16 CONTROL PANEL P/N 00342-00 ............................................................................................... 26
2.17 HYDRAULICS INTERFACE BOARD P/N 00419-00 ............................................................ 26

2 DESCRIPTION OF THE SYSTEM
"Liasys" is a random access, counter-top, computer controlled clinical analysis instrument.

The system can perform 200 tests per hour and has a machine cycle of 18 sec. Its execution time
ranges from a minimum of 48 sec to a maximum of 756 sec, depending on the analysis method
chosen.
The first time the system is used for normal laboratory analyses, the operator must configure the
system based on the specific needs of that laboratory; i.e.: the chemical parameters; the reagents
racks and the normal, calibrated and control values must all be defined.
The daily routine analyses will be carried out according to patient sample arrival in a sequential and
continuous, non-stop manner.
The work list is organized using a loading rack holding 16 patient samples plus a STAT rack for 14
patient samples. Rack loading is non-stop.
The racks can accommodate both test tubes and micro caps. The bar code for primary tubes is
optional.
When the system is first turned on, the analytical unit, the computer and the color-meter lamp are
supplied with low voltage power (1.2 volts). The sampling arm pre-heater is turned off while the
reaction plate heater, the reagents refrigerating unit and the electronic components are turned on.
The "Stand-by" light, placed on the front panel will flash until the reaction plate reaches a
temperature of 36° C. At this point, the "Stand-by" light will stop flashing and will remain
constantly lit.
In the case of system failure or malfunction, the "Ready" light, situated on the front panel of the
instrument, will light up red.
In order to enter the main program, double click on the "Liasys" icon on the computer desktop.
The main menu - "System Monitor" - will appear.
Whenever any system function is launched, the color-meter lamp and the sampling arm pre-heater
will receive regular power.

2.1 ANALYSES CYCLE
2.1.1 REACTION PLATE
The reaction plate of the "Liasys" system contains 60 washable and reusable, plastic cuvettes. The
cuvettes can be removed individually.
Cuvette washing takes place under the reaction plate washing station. There are five positions which
alternate in the washing and drying of the cuvettes.
The basic operating cycle of the reaction plate takes 18 seconds. Said cycle includes: optic reading
of the cuvettes in incubation, aspiration and dispensation of the reagents and samples by the arm,
repositioning of the plate and washing of the cuvettes.
The reactions take place at 37° C. this temperature is constantly maintained by the heater placed
under the reaction plate.
2.1.2 REACTION PLATE CYCLE
After reagents and samples have been placed in cuvette #1, the reaction plate will rotate counter-
clockwise to position #31 so as to bring the first cuvette to be analyzed in front of the color-meter
for reading with one or two wavelengths as required.
The plate will then, moving counter-clockwise, carry out all the readings of any other prepared
cuvettes. After having effected all the readings, the plate will move clockwise to its initial position
minus one cuvette, ready for a new dispensing.
Therefore, the reaction cuvettes will move clockwise 1 - 2 - 3 – 4 for their dispensation and
washing, and counter-clockwise 4 - 3 - 2 - 1 for their reading.

2.2 SAMPLING ARM - OPERATIONAL SEQUENCE
1. The sampling arm lifts up from the washing well and carries out a washing cycle.
2. The arm moves toward the specific reagent container, while the diluter aspirates an air
bubble to separate the rinse column from the reagent.
3. The arm descends into the reagent to the level indicated by the sensor and aspirates the
required volume of reagent.
If the method requires a line prime volume, an extra amount of reagent (not used in the
analysis) will be aspirated before the quantity of reagent necessary for the analysis along
with a smaller air bubble for separation.
4. While the diluter aspirates a second air bubble, the arm rises and then lowers into the wash
well so that it can be washed externally, to minimize cross contamination.
5. The arm moves to the specified sample and aspirates a third air bubble.
6. When the level sensor senses the liquid, the arm stops and aspirates the sample.
7. The arm rises once again, while the diluter aspirates a fourth air bubble to prevent sample
loss.
8. At this point, the arm returns to the wash well in order to clean the outside of the probe and
it aspirates a fifth air bubble.
9. The arm moves to the reaction plate and dispenses reagent and sample in the reaction
cuvette for incubation and reading.
10. The arm returns to the wash well and carries out a probe wash cycle.

SAMPLING SYSTEM
Aspiration of Air
Aspiration of Reagent
Aspiration of Air
(To separate reagent from serum)
To wash well to clean the

probe
Aspiration of air
Aspiration of serum
Aspiration of air
To wash well to clean the

probe
H2O
Aspiration of air
AIR
RGT
Dispensing and mixing in AIR
cuvette AIR
SERUM
To wash well for final
AIR
wash

2.3 WASH STATION
The reaction plate wash station is made up of a series of five small flags situated on one side of the
reaction plate. Said flags are opportunely connected to the valve and pump system for emptying,
washing and drying operations (please see the hydraulic diagram).
2.3.1 WASH STATION CYCLE
The wash station carries out its operations alternating upward and downward movement. In its
downward movement phase the flags are guided in such a manner as to carry out the following
operations:
• The first flag, using the central cannula, removes the reaction mix while the external cannula
dispenses, shower-like fashion, the wash solution (this operation is repeated twice);
• The second flag operates exactly like the first but uses distilled water instead and repeats the
operation three times;
• The third flag dispenses distilled water into the cuvette so that an optics check can be
performed (if the results are negative, the cuvette is discarded);
• The fourth flag aspirates the control water;
• The fifth flag dries the sides.
All these operations are part of the routine operation of the instrument. Every reaction cuvette is
washed at the end of each round of analysis.
The reusability (optical integrity) of each reaction cuvette is always tested before the next round of
analysis.

2.4 ELECTRICAL SCHEMES DESCRIPTION
2.4.1ANALYTICAL CONTROL BOARD (CPU) P/N 00195-01
2.4.2 INTRODUCTION
The ANALYTICAL CONTROL BOARD (C. P. U.), integrated in the LIASYS system is the heart
of the low level, real time, processing management.
2.4.3 GENERAL CHARACTERISTICS
The board has been designed to be a generic control of input/output, governed by an external C.P.U.
or by an internal PC board based platform, in light of possible different future instrument
configurations.
2.4.4 CIRCUIT DESCRIPTION

The following description refers to the [SE]00195-01 Rev. C. electrical wiring diagram.
2.4.5 POWER SUPPLY

• The main power supply VM (24V), filtered by C10 and C12, is taken up via connector J6 and
used to generate the +5 volts; moreover, it is brought back on connectors J9 and J10 to 25
pins
• The 5V is obtained through U4 (LM2575), the Schottky D5 diode, the L1 inductance and
condensers C14 and C15: the entire circuit makes up a step down converter - from 24V to 5V
- with 1 Ampere of current.
• Said power supply is brought back on connector J6 forecasting the possibility that it could
come directly from an external power source (in this case the step-down components are not
mounted, with the exclusion of the C14 and C15 condensers).
• A second power supply, 12.5V, filtered via C9 and C11 is taken up via connector J6, in
addition to being brought back on connectors J9 and J10 to 25 pins and on connectors J13 and
J14 to 10 pins and utilized to generate the 12V for the two flash memories inside the two U1
and U2 microprocessors and the AVCC.
AVCC (5V) used for the operational U23 and U24 and also for the two microprocessors' internal
A/D converters.

2.4.6 MICRO-CONTROLLERS AND BUFFER
In order to quickly and most efficiently manage all the devices, there are two independent micro-
controllers, U1 and U2. Each sends input and output signals to the relative connectors.
All signals which begin with the letter 'P' refer to the Micro 'P' (U1), while those which begin with
the letter 'R' refer to the Micro 'R' (U2).
The two Micros are HITACHI H8/3048, configured in operative mode 7, single chip. Following are
some basic performance features:
- elevated speed in carrying out instruction (2 clock cycles at 16 MHz equal to
125 ns to carry out an addition or subtraction operation between 2 registers);
- 128 KBYTES flash memory;
- 4 KBYTES RAM;
- 70 I/0 signals, almost all having more than one function, plus 8 input only.
- All signals are buffered in input or in output:
- the buffer for the T.T.L. signals is 74HC244;
- the driver for the open-collector signals is ULN2803;
- the operational amplifier LM324 is used for the analogic signals.
The following two tables list the 78 Micro 'P' and Micro 'R' signals. For each the following are
specified:
- the name of the signal on the pin of the Micro (e.g.: P30)
- the type: I for output. 0 for input;
- the buffer used for the input or the output (e.g.: U7);
- the connector onto which the signal is sent (e.g.: J5);
- the name of the buffer input signal (if in input) or at buffer output (if in output);
- a brief description of functioning (e.g.: Motor A enable).

TAB.1 Micro ‘P’ Signals
Micro Signal
I/O Buffer Conn. Function
Signal Connector
P10 I/O R14 Synchronization 1 micro ‘R’
P13 I U21 J9/11 PINP Generic
P14 I/O R10 Synchronization 1 micro ‘P’
P17 O U17 J9/14 PDAR1 Pump Diluter
P20 I U15 J7/21 PINP1 Sample rack 1 in place
P25 I U15 J17/5 PINP6 Sample door switch
P26 I U15 J17/7 PINP7 Stat door switch
P27 I U15 J17/9 PINP8 Sample button
P30 O U7 J7/2 PENA Motor A enable
P31 O U7 J7/4 PENB Motor B enable
P32 O U7 J7/6 PENC Motor C enable
P33 O U7 J7/8 PEND Motor D enable
P34 O U7 J7/10 PDIRA Motor A c.w./c.c.w.
P35 O U7 J7/12 PDIRB Motor B c.w./c.c.w.
P36 O U7 J7/14 PDIRC Motor C c.w./c.c.w.
P37 O U7 J7/16 PDIRD Motor D c.w./c.c.w.
P40 O U9 J7/1 PUT1 ST-BY lamp button (Control Panel)
P41 O U9 J7/3 PUT2 SAMPLE lamp button (Control Panel)
P42 O U9 J7/5 PUT3 STAT lamp button (Control Panel)
P43 O U9 J7/7 PUT4 READY lamp button (Control Panel)
P44 O U9 J7/9 PUT5 Not used
P50 O U8 J7/18 PPDA Motor A power down function
P51 O U8 J7/20 PPDB Motor B power down function
P52 O U8 J7/22 PPDC Motor C power down function
P53 O U8 J7/24 PPDD Motor D power down function
P60 O U14 J7/17 PUT9 Reserved 1
P61 O U14 J7/19 PUT10 Reserved 2
P62 O U21 J9/1 PG1 ADC1 gain A
P63 O U21 J9/5 PG2 ADC2 gain B
P64 O U21 J9/23 PSCLK ADC1-ADC2 serial clock
P65 O U21 J9/10 PTTL Not used

P70 I U23A J17/10 PAN1 External temperature N.T.C. sense

P71 I U23B J9/16 PAN2 Preheater current sense
P72 I U23C J9/25 PAN3 Preheater N.T.C. sense
P73 I U23D J13/9 PAN4 Reagent N.T.C. sense
P74 I U16 J9/3 PFOTO1 Optosensor 1 signal
P80 I U14 J17/11 PINP9 Stat button
P81 I U14 J17/13 PINP10 Ready button
P82 I U14 J17/15 PINP11 St-by button
P83 I U14 J17/16 PINP12 Liquid 1 alarm
P84 I U14 J17/12 PINP13 Liquid 3 alarm
P90 O U25 J1/1 P90OUT Auxiliary TX
P91 O U25 J11/3 X91 RS232 TX
P92 I U25 J1/3 P92IN Auxiliary RX
P93 I U25 X93 RS232 RX
P94 I U16 J9/8 PRDY2 ADC2 data ready
P95 I U16 J9/15 PRDY1 ADC1 data ready
PA0 I U6 J13/8 POSR1 Optosensor reagent 1 signal
PA2 O U8 J7/26 PCLKA Motor A clock
PA4 O U8 J17/2 PCLKB Motor B clock
PA6 O U21 J9/21 PPWM Preheater P.W.M.
PA7 O U17 J13/4 PDAR2 Air pump
PB0 O U8 J17/6 PCLKD Motor D clock
PB1 I U21 J9/6 PLEAK Sampling leak assy
PB2 O U8 J17/4 PCLKC Motor C clock
PB3 I U16 J9/24 PADC1 ADC1 digital output
PB4 I U21 J9/4 PSLI Level sensor
PB5 I U16 J9/2 PADC2 ADC2 digital output
PB6 I MICRO P address
PB7 I U14 J17/14 PINP14 Liquid 2 alarm

TAB.2 Micro ‘R’ Signals
Micro Signal
I/O Buffer Conn. Function
Signal Connector
R10 I/O P14 Synchronization 1 micro ‘P’
R13 I U22 J10/11 RINP Power led opto (3)
R14 I/O P10 Synchronization 1 micro ‘R’
R17 O U17 J10/14 RDAR1 Diluter pump
R20 I U19 J8/21 RINP1 Leak
R21 I U19 J8/23 RINP2 Interlock
R22 I U19 J8/25 RINP3 Not used
R30 O U13 J8/2 RENA Motor A enable
R31 O U13 J8/4 RENB Motor B enable
R32 O U13 J8/6 RENC Motor C enable
R33 O U13 J8/8 REND Motor D enable
R34 O U13 J8/10 RDIRA Motor A c.w./c.c.w.
R35 O U13 J8/12 RDIRB Motor B c.w./c.c.w.
R36 O U13 J8/14 RDIRC Motor C c.w./c.c.w.
R37 O U13 J8/16 RDIRD Motor D c.w./c.c.w.
R40 O U11 J8/1 RUT1 EV1 valve
R44 O U11 J8/9 RUT5 Cover lock
R45 O U11 J8/11 RUT6 MP2 pump
R50 O U12 J8/18 RPDA Motor A power down function
R51 O U12 J8/20 RPDB Motor B power down function
R52 O U12 J8/22 RPDC Motor C power down function
R53 O U12 J8/24 RPDD Motor D power down function
R60 O U20 J8/17 RUT9 Reserved 1
R61 O U20 J8/19 RUT10 Reserved 2
R62 O U22 J10/1 RG1 ADC1 gain A
R63 O U22 J10/5 RG2 ADC2 gain B
R64 O U22 J10/23 RSCLK ADC1-ADC2 serial Clock
R65 O U22 J10/10 RTTL Lamp off

R70 I U24A J18/10 RAN1 External temperature N.T.C. sense

R71 I U24B J10/16 RAN2 Plateheater current sense
R72 I U24C J10/25 RAN3 Plate N.T.C. sense
R73 I U24D J14/9 RAN4 Not used
R74 I U18 J10/3 RFOTO1 Optosensor 1 signal
R81 I U20 J18/13 RINP10 Peristaltic pump A sense
R82 I U20 J18/15 RINP11 Peristaltic pump B sense
R90 O U25 J3/1 R90OUT Auxiliary TX
R91 O U25 J11/3 X91 RS232 TX
R92 I U25 J3/3 R92IN Auxiliary RX
R93 I U25 X93 RS232 RX
R94 I U18 J10/8 RRDY2 ADC2 data ready
R95 I U18 J10/15 RRDY1 ADC1 data ready
RA0 I U6 J14/8 ROSR1 Not used
RA2 O U12 J8/26 RCLKA Motor A clock
RA4 O U12 J18/2 RCLKB Motor B clock
RA6 O U22 J10/21 RPWM Plateheater P.W.M.
RA7 O U17 J14/4 RDAR2 Not used
RB0 O U12 J18/6 RCLKD Motor D clock
RB1 I U22 J10/6 RLEAK Leak plate assy
RB2 O U12 J18/4 RCLKC Motor C clock
RB3 I U18 J10/24 RADC1 ADC1 digital output
RB4 I U22 J10/4 RSLI Not used
RB5 I U18 J10/2 RADC2 ADC2 digital output
RB6 I Micro ‘R’ address
RB7 I U20 J18/12 RINP14 Not used

2.5 PROGRAMMABLE LOGIC

It is made up of U10 (PAL 16V8) and has the following functions:
- Reset generation for the two Micros:

Each Micro is reset: if RESA is present, generated by U5 (DS1233-10), when downloading
begins (RESB signal) or for the entire length of program downloading of the other Micro
(BOOT and SELP signals, the latter is provided by the first serial connector J2).
The relative equations in PALASM format are:

/RESP = /RESA + RESB + BOOT * /SELP
/RESR = /RESA + RESB+ BOOT * SELP
- Generation of X91 associated with one of two TX lines (Micro 'P' P91 and
Micro 'R' R91) to be directed toward the PC both in the operative phase and in the BOOT
phase.
R91 is chosen if SELP is low (downloading phase Micro 'R') or if R10 is low (operative phase
in which Micro 'R', after ascertaining that the channel is free, wants to transmit): in all other
cases X91 brings back P91.
The corresponding equation in PALASM format is:

/X91 = /SELP * /R91 + SELP * (/R91 * /R10 + /P91 * R10)
- Generation of the signals for programming the Micro 'R' flash

Activate PMD2E in the BOOT phase if SELP is high or in the operative phase if Micro 'P'
wants to write in flash (low P66);
Activate PVPPE in the BOOT phase if SELP is high;
Activate RMD2E in the BOOT phase if SELP is low or in the operative phase if Micro 'R'
wants to write in flash (low R66);
Activate RVPPE in the BOOT phase if SELP is low.
The relative equations in PALASM format are:

PMD2E = /P66 + BOOT * SELP
PVPPE = BOOT * SELP
RMD2E = /R66 + BOOT * /SELP
RVPPE = BOOT * /SELP

2.5.1 FLASH PROGRAMMING VOLTAGE

The PMD2E, PVPPE, RMD2E, RVPPE signals pilot 4 U17 Darlingtion sections, whose outputs
(PEMD2, PEVPP, REMD2, REVPP signals) activate, respectively, the PNP Q2, Q3, Q1 and Q4
transistors which bring back the 12V respectively on pin MD2 and pin VPP of Micro 'P'
and Micro 'R'.
2.5.2 SERIAL INTERFACES

The first serial of each Micro (signals P90 and P92 for Micro 'P'; R90 and R92 for Micro 'R') is
dedicated to communicating with its own auxiliary device (respectively J1 and J3).
For the reception channel of the second serial:
- Micro 'P' is linked to serial 1 of the PC (P93←→RX).
- Micro 'R' can be:
• linked to serial 2 of the PC (R93 ←→ RX2 coming from J5) shifting the tin soldered jumper
from pins 2-3 of J12 to pins 2-1
• linked in parallel with those of Micro 'P' and therefore linked to series 1 of the PC (R93 ←→
RX :tin soldered jumper on pins 2-1 f J12).
For the transmission channel of the second serial:

- Micro 'R' is linked to serial 2 of the PC (R91 ←→ TX2).
- Micro 'P' can:
• be linked to serial 1 of the PC (P91 ←→ TX) shifting the tin soldered jumper from pins 2-3 of
J11 to pins 2-1
• share the transmission to the PC with the other Micro (tin soldered jumper on pins 2-1 of J11).
In this case the arbitration between the two Tx signals is carried out in the BOOT phase via
the SELP signal and in the operative phase via the R10 and P1 signals which the two Micros
exchange and which respectively receive on signals P14 and R14 everything governed by
programmable logic).
The two Micros carry out program downloading via J2: in addition to the TX and RX signals also
the following signals are utilized for this function:
- SELP which goes directly on the programmable logic and which if low, signals that it wants
to load the program on Micro 'R'.

- RTS and DTR which after the RS232-TTL conversion become BOOT and RESB and are
brought back on the programmable logic for reset generation and for enabling the
programming voltage for Micros 'P ' and 'R'.
The same program is loaded on the two Micros.
In the operative phase, the format of the communication messages with the PC contains an
address 'number':
- '1' for those messages which can be processed only by Micro 'P', recognized because the
PB6 input is high;
- '2' for those messages which can be elaborated only by Micro 'R', recognized because the RB6
input is low.
The four TTL-RS232 conversions:
• P90-P90OUT: transmission toward Micro 'P's auxiliary device;

• R90-R90OUT: transmission toward Micro 'R's auxiliary device;
• R91-TX2: transmission toward Micro 'R's PC,
• TX5V TX: transmission toward either Micro 'P's or Micro 'R's PC,
and the four RS232-TTL conversions:
• P92IN-P92: reception from the auxiliary device for Micro 'P';

• R92IN-R92: reception from the auxiliary device for Micro 'R';
• RX2-R93: reception from the PC for Micro 'R';
• RX-P93-R93: reception from the PC for Micros 'P' and 'R'
are carried out through the U25 and U26 (MAX202) devices.
2.5.3 CLOCK
The system clock is supplied by quartz at 16MHz X1 connected to the Micro 'P' internal oscillator
pins EXTAL and XTAL: output TTL of this oscillator (U1/61) is brought back on input XTAL of
the Micro 'R' oscillator.

2.5.4 LED
There are two LEDs
- LD1: green LED for VM presence
- LD2: green LED for +12.5V presence.
2.5.5 BUZZER
The acoustic signals are channeled to the LS1 buzzer and can be activated via the PDAR2 and/or
RDAR2 signals of the two Micros.
2.6 STEPPER MOTORS DRIVER BOARD P/N 00188-01
2.6.1 INTRODUCTION
The "STEPPER MOTORS DRIVER BOARD" is the interface between the control board and the
stepper motors.
2.6.2 GENERAL CHARACTERISTICS

This board has been designed to be used in various systems or sub-groups which require mechanical
movement .
It can manage up to four stepper motors and has an open collector (Darlington) interface for 16
signals, whose output can be sent to various devices including electro-valves, continuous current
motors.
2.6.3 CIRCUIT DESCRIPTION

The following description refers to the [SE]00188-01 Rev.B. electrical wiring diagram
2.6.4 POWER SUPPLY

The main power supply, VM (24 V), applied via J3 and indicated by the lit green D22 LED, is used
for the stepper driver power portion (TA8435H).
The secondary power supply, 12.5 Volts, also comes through connector J3, is filtered by condenser
C13, monitored via the green D21 LED and used to generate the 5 Volts necessary for the stepper
driver logic section and for the pull-up resistors.
The 5 volts are obtained from the 12.5 Volts through U5 (LM7805) and the C14 filter condenser.

2.6.5 STEPPER MOTORS DRIVER
The four drivers are represented by the U1, U2, U3 and U4 components.
Following is a description of the functioning of the first (U1) of the four identical circuits.
Later on in this manual a brief description of the functioning of the NMB SDI-C403 device or
TOSHIBA's equivalent TA8435H will be provided.
The driver under examination is made up of:
A logic section for decoding signals arriving from the control board, via the J4 and J7 connectors,
which uses the following inputs:
DIRA (pin 5): determines the direction of the rotation of the motor;
RES (pin 2): reset;
CLKA (pin 6): controls phase excitation frequency: the pilot mode depends on the M1A (pin 8)
and M2A (pin 9) inputs, set via the SW1DIP SWITCH according to the following table:
M2A M1A Function

0(on) 0(on) 1 full step
0(on) 1(off) 1/2 step
1(off) 0(on) 1/4 step (default setting)
1(off) 1(off) 1/8 step
Please note that the other clock input (pin 7) is connected to the +5 volts.
Use of the 1/4 and 1/2 steps, with respect to the full step, make it possible to lessen vibration and
improve positioning accuracy.
A power section, (made up of 2 H-shaped bridges), that generates the voltage to be applied to the
two stepper motor windings (AM62, AM62N, BM62, and BM62N respectively on pins 23, 20, 19,
and 16) on connector J1.
The latter are supplied when the RES signal is high and the ENA signal (coming from the control
board, via connector J4) is at a low logic level.
The state of the ENA signal level, and therefore that of the relative Stepper Motor driver, is
indicated by the yellow D17 LED.
The D1, D3, D5 and D7 Schottky diodes are to protect the internal power circuits.

A power control and monitoring section which, via the C5 (3.3nF) condenser generates a circa 60
kHz internal oscillation frequency used to control the Pulse Width Modulation (PWM).
Said control is affected by:
the voltage levels, produced by the potential drop on the R1 and R2 power resistors, present on pins
21 and 18;
the PDA (pin 10) signal, arriving from the control board (via connector J4), which conditions the
internal switching logic intervention threshold; to 0.8 volts if it is kept at a high logic level and 0.5
volts if at a low logic level.
2.6.6 OPEN COLLECTOR DRIVERS
Two integrated circuits are present on the board, U6 and U7 (ULN2803), and constitute an open
collector interface for 16 signals:
10 of which come from the control board (UT1…UT10) via J4 with their outputs connected to J2;
6 gathered via J5 and placed in output on J6.
These outputs are used to pilot various types of power devices: continuous current motors,
solenoids, electrovalves, etc.
2.7 TA 8435 H
2.7.1 INTRODUCTION
TA 8435 H controls the PWM for setting the current of the Stepper Motor windings to a constant
value. The device is an integrated circuit for micro-step piloting, used in order to have an efficient
and low vibration control over the Stepper Motors.
2.7.2 MICRO-STEP PILOTING
TA 8435 H controls a Stepper Motor in micro-steps; with a maximum resolution of 1/8 of a step.
The current levels of the A and B phases are regulated in micro-steps within the integrated circuit in
such a manner that the size and rotation angle of the vector between the micro-steps remains
constant. When the clock signals are placed in input (CK1, CK2), the Stepper Motor will rotate in
micro-steps.

2.7.3 CONTROL OF PULSE WIDTH MODULATION AND OUTPUT CURRENT
Output current pathway flow diagram (PWM control)

TA 8435 H controls PWM, placing the upper transistor in an on/off switching mode. In this case
current flow is as illustrated below:
Control of the output current via the detection resistor of the output and input current REF-IN:
the motor current (maximum current for micro-step piloting I∅), is regulated as shown in the
following equation, using the REF-IN in-put and the detection resistor of the external current RNF:
I∅ = VREF/ RNF
Where:
for REF-IN = HIGH ⇒ VREF = 0.8 V
for REF-IN = LOW ⇒ VREF = 0.5 V

2.7.4 CONTROL LOGIC
Clock input for rotation direction control

To switch the rotation direction from forward to backward there are two clock inputs: CK1 and
CK2, and input CW/CCW and the first of the two control methods are shown in the diagram below:
2.7.5 1/2 STEP PILOTING

The selection between: full step, 1/2 step. 1/4 step and 1/8 step, is carried out with binary coding on
the two M1 and M2 inputs.
2.8 SAMPLING INTERFACE ASSY P/N 00192-01
The "Sampling Interface Assy" board (code 00192-01) controls the arm, the diluter, the pre-heater
and wash well leakage.
The Home Sensors (vertical, external arm, internal arm and diluter) are connected to the board via
the J9, J10, J11 and J13 connectors.
The Vertical Home, External Arm Home and Internal Arm Home LEDs are placed in series with
their power absorption control via the ALFD3 signal, connected to J2 Pin 11 that reports
information on the Analytical Control Board (CPU).

Information from the leakage sensor enters on the J12 connector, is processed by U1A, exits on J2
Pin 6 and arrives through J10 to the Analytical Control Board for management.
The N.T.C. resistor detects the temperature of the pre-heater and is connected to J7 on Pins 5 and on
Pins 3 and 4 (ground). These Pins are connected to the U2B amplifier, whose output via J2 Pin 25
carries information to the CPU board (Analytical Control Board) on input AN2 (P72) of the Micro
R U2.
The pre-heater heating element is connected via J7 Pins 6 and 7 and is powered by Q1 which is
controlled by P.W.M. generated by the Micro R of the CPU. Furthermore, it can be excluded by
switching the LS1 relay through Q6, conditioned by the OFFPR signal generated by the CPU.
2.8.1 SAMPLING INTERFACE ASSY P/N 00192-01
INPUT:
- Vertical Home (J9)

- External Arm Home (J10)
- Internal Arm Home (J11)
- Diluter Home (J13)
- NTC + (J7 Pin 5)
- LEAK (J12)
- PDIL (coming from the CPU) (J2 Pin 14)
OUTPUT:
- Vertical Arm (J3)

- Internal Arm (J4)
- External Arm (J5)
- Diluter (J6)
- Diluter Pump (J8)
- Pre-heater (J7)
- LEAK (J2)

2.9 LEVEL SENSOR BOARD 1-2 P/N 30-0005-XX
The integrated U4 circuit generates a trigger signal for U3 where the sampling probe is connected
on Pin 6 via C2.
The capacity variation, produced by the contact between the sampling probe and a liquid,
determines a voltage variation on Pin 3 of U3, which ranges from a value close to zero to one which
tends towards + 5 V.
This signal is then further processed by U2 and Q1 and is placed in output from the board on J2
Pin 1.
2.10 REACTION TRAY INTERFACE P/N 00193-01
This board controls various functions:

J2, J3, J4 and J5 are the input connectors for the opto sensors of the filters wheel, of the washing
station, of the reaction plate and of the cuvette holders. This information is transferred to the
Analytical Control Board (CPU) using the OS2, OS3, OS4 and OSB signals via J1.
The ALFD3 signal controls power supply to the series of the three emitting diodes contained in the
opto sensors connected to J2, J3 and J4. ALFD1 functions in a like manner relative to the opto
sensor connected to J5.
Liquid leakage signal enters on J7 and is processed by U1A, whose output (LEAKP) returns via J1
on the CPU.
Plate temperature is detected by the N.T.C. sensor connected to J8 and sent to the Micro P.
The cuvette holder solenoid is turned on by activating the BCU signal produced by a ‘Darlington’
controlled by the Micro P.
The PWMP signal, that controls the reaction plate heater, is also produced by the Micro P. Its power
absorption is controlled via the P71 signal.
Furthermore, the board distributes, toward other modules, input and/or output signals managed by
the CPU.
24V and 12.5V arrive to the board. The 5V (U5) used for the amplifiers and the negative voltage
(U4) that is sent to both the Sample and Reference PRE-AMPL/ADC are produced here.

2.11 REACTION TRAY INTERFACE P/N 00193-01
INPUT:
- Home Washing Station (J3)

- Home Filters Wheel (J2)
- Home Reaction Tray (J4)
- Home Cuvette Holder (J5)
- Reaction Tray Leak (J7)
- Reaction Tray Temperature Sensor (J8)
- (PWMP (PWM for the Heater) (J1)
- BCU (Activation of the Cuvette Holder Solenoid) (J1)
- OFFPL (Activation of the Lamp, Feedthrough (J1)
OUTPUT:
- Cuvette Solenoid Holder (J6)

- Reaction Tray Heater (J10)
- ALFD3 (three light emitting diodes in series) (J1)
- OS3 (Washing Station Home Sensor) (J1)
- OS2 (Filters Wheel Home Sensor) (J1)
- OS4 (RTC Tray Home Sensor) (J1)
- OSB (Cuvette Holder Home Sensor) (J1)
- ALFD1 (Cuvette Holder Sensor Diode Control) (J1)
- P72 (Reaction Plate Temperature) (J1)
- LEAKP (Reaction Plate Leak) (J1)
- P71 (Reaction Tray Heater Power Control) (J1)
- V- (Voltage –12V for PRE-AMPL/ADC) (J1)
- PWRES (Heater Power) (J1)
- OFFPL (Lamp Activation) (J9)

2.12 REACTION CHAMBER MOTOR INTERFACE P/N 00341-00
This board distributes the motors signals - those of the: filters wheel (J2), washing station (J3), and
reaction plate (J4), part of the Reaction Group Assy.
The connector J1 interfaces the Stepper Motors Driver Board (30-00188-01) which is managed by
the Micro P of the CPU via connector J1. Signal distribution for the three motors is carried out via
the three above cited connectors.
2.13 PRE-AMPL/ADC P/N 00107-00
The FD1 photodiode detects light energy arriving from the optics. The amplifier (U2) amplifies this
signal and sends it to the A/D serial converter ADS 1250.
Both U1 and U4 produce the +5V; the former for the analogic segment and the latter for the digital
segment. U5 generates the –5V and U6 the voltage reference VREF. Y1 is the oscillator connected
to the U3 clock.
The G0 and G1 signals determine the U3 gain (1, 2, 4 or 8).
The Board interfaces with the Reaction Tray Interface Board (00193-01) via the JP1connector that
sends the signals to the Micro P.
2.14 PHOTOMETER LAMP PWS P/N 30-0004-00
This board powers the 6V 10W halogen lamp.

The main device of this circuit is a type LT 1083CP U1 integrated stabilizer which, under normal
operative conditions, is regulated, via the 1 Kohm P1 potentiometer, to 6V for the lamp.
When the system is in Stand-by, the lamp is not turned off. Instead, the voltage is lowered from 6V
to 1.2V by the Micro P which acts on the Q1 transistor via the OFFPL signal. This is done in order
to not over-stress the lamp by turning it on and off frequently.

2.15 SAMPLE RACKS IDENTIFICATION BOARD P/N 00446-00
This board checks that the samples racks are inserted correctly.
The sole power supply used in the board is Vcc (5V), derived from VM (24V) that arrives to the
board via the J1 connector.
Racks sensing is carried out via OPB848 type opto sensors U1, U2, U3, U4, and U5. These sensors'
outputs arrive to connector J7 of the Control Panel (30-00342-00) via J1. They exit through the
same J1 and go towards J2 of the Stepper Motors Driver Board (30-00188-01) and from here, via J4
and J7 enter on J7 and J17 of the Analytical Control Board (CPU) (30-00195-01) for the
management functions performed by the Micro P (U1).
2.16 CONTROL PANEL P/N 00342-00
This board controls the levels of the Rinse, Water and Cleaning Solution bottles, the closure of the
sample rack and reagent rack doors, the buttons – indicators placed on the front panel of the
instrument – and the acoustic alarm buzzer.
The St-by and Ready buttons are only lit indicators. The STAT button, instead, actually turns off
the acoustic alarms.
Signals regarding the presence of the five sample racks pass through this board.
2.17 HYDRAULICS INTERFACE BOARD P/N 00419-00
This board is used to distribute the following DARLINGTON commands:

EV1, EV3, EV4, EV5, EV6, MP2, MP3, MP4, INTERLOOK, or INTERLOCK.
In addition, this board is used to distribute the peristaltic pump’s motor signals.
U2 generates +5 V , U1 is the operator that keeps the leak (D9 on = leak alarm) under control.
The parts connected to J9 and J10 are designed to monitor the fluctuating liquids of the peristaltic
pump’s two canals.
The microswitch signal transits to J8 which identifies if the cover is open (IL=1) or closed (IL=0).

Chapter 03 - INSTALLATION
CHAPTER 03
- INSTALLATION -
INDEX
3.1 UNPACKING ................................................................................................................................ 2
3.2 INSTALLATION........................................................................................................................... 4
3.2.1 INSTALLATION SITE SPECIFICATIONS ............................................................................. 4
3.2.2 ELECTRIC CURRENT REQUIREMENTS .............................................................................. 4
3.2.3 CONNECTION OF THE ACCESSORIES ................................................................................ 5
3.2.3.1 POWER SUPPLY.................................................................................................................... 5
3.2.3.2 COMPUTER - INSTRUMENT CONNECTION.................................................................... 5
3.2.4 ATTENTION .............................................................................................................................. 6
3.2.5 SYMBOLS.................................................................................................................................. 6
3.2.6 REGULATORY COMPLIANCE............................................................................................... 6

3.1 UNPACKING
The LIASYS is packed and delivered in two separate wooden crates: one contains the analyzer itself
and the other the computer, along with its accessories. In the event that the order not include the PC
component, packing and delivery will involve one wooden crate plus a corrugated cardboard box.
The packing has been expressly studied and designed to insure maximum protection of the contents
during shipping and handling. It is therefore extremely important that the crate(s)/box be carefully
examined upon delivery in order to ascertain their integrity. Special attention should be dedicated to
examining the color of the “Shock Watch” glued to the crates, which must show the color ‘white’.
A ‘red’ “Shock Watch” indicates that the crate(s) have experienced some sort of ‘shock’ during
handling, transport and/or delivery. This fact must be noted by the courier on the delivery note, as
must any and all visible external damage (for example: holes, dents, rips or tears, water marks, etc.)
evident at the moment of delivery. This will simplify matters in the event of any future claims for
damages.
Upon arrival of the crate(s)/box, take out the

delivery note and make sure that all the items
on the packing list are included in the crates
and are undamaged. Make sure the series
number on the delivery note/packing list
corresponds to that impressed on the plate on
the right side of the instrument.
Open the crate(s)/box from the top and very

carefully take out:
- the instrument;
- the computer and accessories.
MAKE SURE THAT THE UNPACKING IS CARRIED OUT BY TWO PEOPLE.

Do not discard the delivery crate(s)/box or the packing material until the correct functioning of the
instrument has been ascertained.
Remove all the items from the crate(s)/box very carefully.
Remove the adhesive tape from the cover of the samples and reagents housing, from the front
panels and from the samples and reagents racks.
Before connecting the "Liasys", remove the protective packing material placed under the sampling
arm and under the wash station group.
Warning: in the event that it is necessary to repack any or all of the delivered item(s), the following
procedures must be carefully followed:
¾ Reposition the protective packing material under the sampling arm and under the wash station
group.
¾ Tape down (using masking tape if possible) the cover of the samples and reagents housing, the
front panels, and the samples and reagents racks.
¾ Remove the probe from the sampling arm and place it inside a cuvette. Then cap the cuvette
and tape the cap down.
¾ Be very careful to not bend the wash station cannulas when repositioning the protective
packing material.
¾ Fill the empty spaces around the accessories packed in the crate using “pluriballs” or other
suitable packing material.

3.2 INSTALLATION
The LIASYS must only be installed by a qualified technician who has been authorised and trained
to do so. During its installation the system will be checked once again to ensure correct functioning.
The persons who are required to operate the LIASYS system must have received the adequate
training. This should also include the "know-how" of the normal maintenance for the instrument. A
description of the maintenance will be found in Chapter 7 of this manual.
LIASYS is a complex system, and it is therefore extremely important that it is correctly installed in
order to fully guarantee fine performance. If the installation and use directions, given in this
manual, are not correctly followed and/or security indications are not respected, AMS cannot
guarantee correct functioning of the instrument. Apart from this, the security of the operator could
be placed at risk.
3.2.1 INSTALLATION SITE SPECIFICATIONS

Ascertain that the LIASYS system is not exposed to direct sunlight, draughts, dust or strong
magnetic fields. In addition, please take note of the following conditions required for the location of
the installation:
USE In covered and dry place

DEGREE OF POLLUTION 2
INSULATION CLASS I
INSTALLATION CATEGORY II
TEMPERATURE between18°- 30°C
HUMIDITY 20% ÷ 85%
ALTITUDE Max 3000 m
LOCATION Shelf or table with a minimum surface of 110x70 cm
stable and free of vibration
VENTILATION Leave a minimum distance of 10 cm around the
instrument to permit air circulation . Make sure that
the fan (situated under the reagent compartment) is
not blocked by any object
3.2.2 ELECTRIC CURRENT REQUIREMENTS

The power voltages to which the instrument is adapted are indicated on the left-hand side (see fig.
1). It must be plugged into a plug of the correct voltage.
VOLTAGE 90 ÷ 250 Vac 47/63 Hz ± 10%

FUSES 6.3 Amp/T - 6.3 x 32

NOTE: IT IS ADVISABLE TO MAINTAIN THE MAXIMUM STABILITY OF THE ELECTRICAL CURRENT IN THE
LABORATORY. WHERE THIS IS NOT POSSIBLE OR ASCERTAINABLE, USE OF THE FOLLOWING
SUPPLEMENTARY DEVICES IS RECOMMENDED:
ELECTRONIC STABILIZER
Used to stabilise the electric voltage in the laboratory. Any stabiliser with a power
potential greater than 0.5 KW, currently available on the market, can be used.
NO-BREAK MODULE UPS - (Uninterrupted Power Supply)

This module provides two important functions:
- stabilises the main-line power
- supplies current to the instrument in case of a main-line power failure.
3.2.3 CONNECTION OF THE ACCESSORIES

3.2.3.1 POWER SUPPLY
Fig. 1 Plug (use the feeder cable supplied with the instrument).
The sticker indicates the power supply voltage and the values of the fuses.
3.2.3.2 COMPUTER - INSTRUMENT CONNECTION

The instrument and the Personal Computer are connected by one serial RS232 standard cable
(Cod. 9-35 0055.01), which provides the hardware support for the communication

3.2.4 ATTENTION
The following label is found at the rear of the instrument.
NOTE: THE REAR PANELS OF THE INSTRUMENT MUST NEVER BE OPENED WITHOUT HAVING FIRST
SWITCHED THE INSTRUMENT OFF AND DISCONNECTED THE ELECTRICITY CABLE.
THE MAINTENANCE AND CLEANING PROCEDURES FOUND IN CHAPTER 07 OF THIS MANUAL

MUST BE RESPECTED AT ALL TIMES. REMEMBER TO FOLLOW THE DECONTAMINATION
PROCEDURE IN CASE OF INSTRUMENT REMOVAL (SEE CHAPTER 07) .
3.2.5 SYMBOLS
ATTENTION: READ THE INSTRUCTIONS IN THE USER MANUAL
TERMINAL OF TOTAL MASS PROTECTION (CONDUCTOR)
3.2.6 REGULATORY COMPLIANCE
The LIASYS instrument complies with:
Safety :
• EN 61010 - 1 : 1993 + A2 : 1995 therefore meeting the essential requirements of L.V.D.
73/23/EEC and 93/68/EEC
EMC :
• EN 61326-1 therefore meeting the essential requirements of Electromagnetic
Compatibility Directive 89/336/EEC - 92/31/EEC

Chapter 04 – ELECTRICAL SCHEMES AND DRAWINGS
CHAPTER 04
- ELECTRICAL SCHEMES AND DRAWINGS -
INDEX
INTERCONNECTION DIAGRAM LIASYS [SB] 16-00457-XX

LIASYS NEW [MA] 16-00457-00
HYDRAULIC DIAGRAM [SI] 00457-00
COMPLETE ARM ASSY 9-MA-15-0021-02
DILUTER ASSY 9-MA-15-0007-03
REACTION CHAMBER ASSY 9-MA-15-0008-01
WASHING STATION PUMPS [MA] 15-00406-00
ELECTRONIC CONTROLLER ASSY [MA] 10-00202-01
SCHEMATIC POWER SUPPLY 9-SE-15-0014-01
ANALYTICAL CONTROL BOARD [MA] 30-00195-01

ANALYTICAL CONTROL BOARD [SE] 00195-01
STEPPER MOTORS DRIVER BOARD [MA] 30-00188-01

STEPPER MOTORS DRIVER BOARD [SE] 00188-01
SAMPLING INTERFACE ASSY [MA] 00192-01

SAMPLING INTERFACE ASSY [30] 00192-01

Chapter 04 – ELECTRICAL SCHEMES AND DRAWINGS
LEVEL SENSOR BOARD 9-30-0005-01

LEVEL SENSOR BOARD 9-30-0005-02
REACTION TRAY INTERFACE [MA] 00193-01

REACTION TRAY INTERFACE [30] 00193-01
REACTION CHAMBER MOTOR INTERFACE [MA] 00341-00

REACTION CHAMBER MOTOR INTERFACE [30] 00341-00
PRE-AMPL/ADC [MA] 30-00107-XX

PRE-AMPL ADC [MA] 00107-00
PHOTOMETER LAMP PWS 9-[MA]-30-0004-00

LAMP PWS BOARD 9-ME-30-0004-00
SAMPLE RACKS IDENTIFICATION BOARD [MA] 30-00446-00

SAMPLE RACKS IDENTIFICATION BOARD [MA] 00446-00
CONTROL PANEL [MA] 00342-00

CONTROL PANEL [30] 00342-00
HYDRAULICS INTERFACE BOARD [30] 00419-00

HYDRAULICS INTERFACE BOARD [SE] 00419-00
ALIMENTATION CABLE [MA] 50-00356-00
LEVEL SENSOR ASSY [SE]10-01478-00

LIASYS
COMP. INTER. WASTE SENS.
P. M0
µP4 µP2 µP3
EV1 EV3 EV4 EV5 EV6
A B C D E F
ALARM RESET ST-BY RUN / READER ON

P. M0
µP4 µP2 µP3
EV1 EV3 EV4 EV5 EV6
A B C D E F
1 2 3 4 5 6 7 8
AVCC 12.5V Q1
X1 AVCC 12.5V Q2 R1 2K2 D1 LED 12.5V Q3 PNP RMD2
R2 1 16M PNP PMD2 PNP PVPP
XTAL EXTAL VM 12.5V C2 C4 R3 TO92
Q4
C7 R4 TO92 R6 1K D2 LED PNP RVPP R5 TO92 0.1u 10K
C3 10K 10K 10uF
C5
P84
P83
P82
P81
P80
P77
P76
P75
P74
P73
P72
P71
P70
12.5V
PA7
PA5
PA4
PA2
PA0
PA6
PA3
PA1
C6 10uF R8 500R R7 TO92 R9
12p
R84
R83
R82
R81
R80
R77
R76
R75
R74
R73
R72
R71
R70
12p
RA7
RA6
RA5
RA4
RA3
RA2
RA1
RA0
0.1u EPMD2 10K PEVPP
R12 ERMD2
J1 REVPP 500R R11 500R
100
99
98
97
96
95
94
93
92
91
90
89
88
87
86
85
84
83
82
81
80
79
78
77
76
P90OUT
PMD2 1 500R
1 75
100
99
98
97
96
95
94
93
92
91
90
89
88
87
86
85
84
83
82
81
80
79
78
77
76
VCC VCC MD2 2
PB0 P92IN
P80
P75
P74
P73
P72
P71
P70
2 74 VCC
GND
PB0/TIOCA3/TP8 MD1 3 SERIAL 1
VREF
PB1 U3 RMD2
AVCC
AGND
3 PB1/TIOCB3/TP9 MD0 73 VCC VCC 1 VCC MD2 75
PB2 P66 H3 ISE J2 LM2940C RB0 VCC
P80
P75
P74
P73
P72
P71
P70
A A
P84/CS0
P83/CS1
P82/CS2
P81/CS3
P77/DA1
P76/DA0
4 72 2 74
GND
PB2/TIOCA4/TP10 P66/LWR PB0/TIOCA3/TP8 MD1
VREF
PB3 P65 J3 RB1 VCC
AVCC
AGND
5 PB3/TIOCB4/TP11 P65/HWR 71 6 3 PB1/TIOCB3/TP9 MD0 73
PA7/TIOCB2
PB4 P64 R90OUT TX RB2 R66
P84/CS0
P83/CS1
P82/CS2
P81/CS3
P77/DA1
P76/DA0
6 PB4/TOCXA4/TP12 P64/RD 70 1 5 4 PB2/TIOCA4/TP10 P66/LWR 72
PB5 7 69 P63 RX RB3 5 71 R65
IN
GND
OUT
PB5/TOCXB4/TP13 P63/AS 2 4 PB3/TIOCB4/TP11 P65/HWR
PA7/TIOCB2
PA6/CS4/TIOCA2
PA5/CS5/TIOCB1
PA4/CS6/TIOCA1
PB6 8 68 R92IN DTR RB4 6 70 R64
PA1/TEND1/TCKB
PA0/TEND0/TCKA
PB6/CS7/R0/TP14 VCC VCC 3 3 PB4/TOCXA4/TP12 P64/RD
PA3/TIOCB0/TCKD
PA2/TIOCA0/TCKC
PB7 9 67 XTAL RTS R14 RB5 7 69 R63
PB7/DREQ1/TP15 XTAL 2 PB5/TOCXB4/TP13 P63/AS
1
2
3
PA6/CS4/TIOCA2
PA5/CS5/TIOCB1
PA4/CS6/TIOCA1
PVPP 10 66 EXTAL H 3 BAR CODE SELP RB6 8 68
PA1/TEND1/TCKB
PA0/TEND0/TCKA
VPP/RESO EXTAL 1 PB6/CS7/R0/TP14 VCC VCC
PA3/TIOCB0/TCKD
PA2/TIOCA0/TCKC
11 65 READING 12.5V AVCC RB7 9 67
P90 GND GND VCC H6 10K RVPP PB7/DREQ1/TP15 XTAL E
12 P90/TX0 NMI 64 10 VPP/RESO EXTAL 66
P91 13 63 RESP J4 11 65
P92 P91/TX1 [PIATTO] RES SELP VM R90 GND GND
14 MICRO P 62 VCC 12.5V 12 64 VCC
P93 P92/RX0 STBY E 2 R91 P90/TX0 NMI RESR
15 P93/RX1 E 61 1 13 P91/TX1 RES 63
P94 16 ADRMICRO 1 60 P62 J5 C9 R92 14 MICRO R [BRACCIO] 62
P94/SCLK0 P62/BACK P92/RX0 STBY VCC
P95 17 59 P61 TX2 H2 + C11 C10 + C12 R93 15 61
P40 P95/SCLK1 P61/BREQ P60 1 470uF/50 R94 P93/RX1 E R62
18 58 16 ADRMICRO 0 60
P41 P40/D0 P60/WAIT 2 RX2 0.1u 470uF/50 0.1u R95 P94/SCLK0 P62/BACK R61
19 P41/D1 GND 57 3 17 P95/SCLK1 P61/BREQ 59
P42 20 56 P53 R40 18 58 R60
P43 P42/D2 P53/A19 P52 H3 SERIAL 2 R41 P40/D0 P60/WAIT
21 P43/D3 P52/A18 55 19 P41/D1 GND 57
22 54 P51 U4 R42 20 56 R53
P44 GND P51/A17 P50 R43 P42/D2 P53/A19 R52
23 P44/D4 P50/A16 53 VM 1 VCC 21 P43/D3 P52/A18 55
P45 24 52 P27 22 54 R51
P46 P45/D5 P27/A15 P26 2 CX6 R44 GND P51/A17 R50
25 P46/D6 P26/A14 51 3 23 P44/D4 P50/A16 53
D5 0.1u R45 24 52 R27
U5 L1 1N5818 4 R46 P45/D5 P27/A15 R26
5 25 P46/D6 P26/A14 51
470u U6 74HC244
P47/D7
P30/D8
P31/D9
P32/D10
P33/D11
P34/D12
P35/D13
P36/D14
P37/D15
VCC
P10/A0
P11/A1
P12/A2
P13/A3
P14/A4
P15/A5
P16/A6
P17/A7
GND
P20/A8
P21/A9
P22/A10
P23/A11
P24/A12
P25/A13
RESA 1 LM2575 PA0 POSR1
2 18 1Y1 1A1 2
U1 PA1 16 4 POSR2
P47/D7
P30/D8
P31/D9
P32/D10
P33/D11
P34/D12
P35/D13
P36/D14
P37/D15
VCC
P10/A0
P11/A1
P12/A2
P13/A3
P14/A4
P15/A5
P16/A6
P17/A7
GND
P20/A8
P21/A9
P22/A10
P23/A11
P24/A12
P25/A13
3 1Y2 1A2
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
H83048 PA3 14 6 POSR3
VCC DS1233-10 VCC J6 VM VCC PA5 1Y3 1A3 POSR4
QFP100-4 12 1Y4 1A4 8 U2
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
B RESET CX5 12.5V RA0 9 11 ROSR1 H83048 B
P47
P30
P31
P32
P33
P34
P35
P36
P37
P10
P11
P12
P13
P14
P15
P16
P17
P20
P21
P22
P23
P24
P25
0.1u 1 RA1 2Y1 2A1 ROSR2 QFP100-4
+ C14 7 13
2 C15 470uF/50 RA3 2Y2 2A2 ROSR3 VCC
3 5 2Y3 2A3 15
VCC
R47
R30
R31
R32
R33
R34
R35
R36
R37
R10
R11
R12
R13
R14
R15
R16
R17
R20
R21
R22
R23
R24
R25
J7 CON26A VCC VCC 0.1u RA5 3 17 ROSR4 J8 CON26A

VCC VCC 4 VCC 2Y4 2A4 VCC
R14
R15
R16
R10
R11
R12
PUT1 PENA VCC 20 1 CX12 VCC RUT1 RENA
PUT2 1 2 PENB CX7 CX8 5 CX9 CX10 VCC VCC 1OE CX11 0.1u CX13 RUT2 1 2 RENB
19
P14
P15
P16
P10
P11
P12
PUT3 3 4 PENC 0.1u 0.1u 6 0.1u 0.1u 2OE 0.1u 0.1u RUT3 3 4 RENC
PUT4 5 6 PEND U7 74HC244 U8 74HC244 H6 U9 74HC244 U11 74HC244 U12 74HC244 U13 74HC244 RUT4 5 6 REND
U10 16V8
PUT5 7 8 PDIRA PENA P30 PPDA P50 PUT1 P40 I0 RUT1 R40 R50 RENA R30 RUT5 7 8 RDIRA
9 10 18 1Y1 1A1 2 18 1Y1 1A1 2 18 1Y1 1A1 2 1 I0 F0 12 18 1Y1 1A1 2 RPDA 18
1Y1 1A1 2 18 1Y1 1A1 2 9 10
PUT6 PDIRB PENB 16 4 P31 PPDB 16 4 P51 PUT2 16 4 P41 BOOT 2 13 X91 RUT2 16 4 R41 RPDB 16 4 R51 RENB 16 4 R31 RUT6 RDIRB
PUT7 11 12 PDIRC PENC 1Y2 1A2 P32 PPDC 1Y2 1A2 P52 PUT3 1Y2 1A2 P42 P91 I1 F1 PMD2E RUT3 1Y2 1A2 R42 1Y2 1A2 R52 RENC 1Y2 1A2 R32 RUT7 11 12 RDIRC
13 14 14 1Y3 1A3 6 14 1Y3 1A3 6 14 1Y3 1A3 6 3 I2 F2 14 14 1Y3 1A3 6 RPDC 14
1Y3 1A3 6 14 1Y3 1A3 6 13 14
PUT8 PDIRD PEND 12 8 P33 PPDD 12 8 P53 PUT4 12 8 P43 P66 4 15 RMD2E RUT4 12 8 R43 RPDD 12 8 R53 REND 12 8 R33 RUT8 RDIRD
PUT9 15 16 PPDA PDIRA 1Y4 1A4 P34 PCLKA 1Y4 1A4 PA2 PUT5 1Y4 1A4 P44 R91 I3 F3 RVPPE RUT5 1Y4 1A4 R44 1Y4 1A4 RA2 RDIRA 1Y4 1A4 R34 RUT9 15 16 RPDA
17 18 9 2Y1 2A1 11 9 2Y1 2A1 11 9 2Y1 2A1 11 5 I4 F4 16 9 2Y1 2A1 11 RCLKA 9
2Y1 2A1 11 9 2Y1 2A1 11 17 18
PUT10 PPDB PDIRB 7 13 P35 PCLKB 7 13 PA4 PUT6 7 13 P45 R66 6 17 PVPPE RUT6 7 13 R45 RCLKB 7 13 RA4 RDIRB 7 13 R35 RUT10 RPDB
PINP1 19 20 PPDC PDIRC 2Y2 2A2 P36 PCLKC 2Y2 2A2 PB2 PUT7 2Y2 2A2 P46 R10 I5 F5 RESP RUT7 2Y2 2A2 R46 2Y2 2A2 RB2 RDIRC 2Y2 2A2 R36 RINP1 19 20 RPDC
21 22 5 2Y3 2A3 15 5 2Y3 2A3 15 5 2Y3 2A3 15 7 I6 F6 18 5 2Y3 2A3 15 RCLKC 5
2Y3 2A3 15 5 2Y3 2A3 15 21 22
PINP2 PPDD PDIRD 3 17 P37 PCLKD 3 17 PB0 PUT8 3 17 P47 SELP 8 19 RESR RUT8 3 17 R47 RCLKD 3 17 RB0 RDIRD 3 17 R37 RINP2 RPDD
PINP3 23 24 PCLKA 2Y4 2A4 2Y4 2A4 2Y4 2A4 I7 F7 2Y4 2A4 2Y4 2A4 2Y4 2A4 RINP3 23 24 RCLKA
25 26 VCC 20 VCC 1OE 1 VCC 20 VCC 1OE 1 20 VCC 1OE 1 RESA 9
I8 VCC 20 20 VCC 1OE 1 VCC 20 VCC 1OE 1 VCC 20 VCC 1OE 1 25 26
19 19 VCC 19 RESB 11 10 VCCVCC 19 19 19
2OE 2OE 2OE I9 GND 2OE 2OE 2OE
J17 CON18A U14 74HC244 U15 74HC244 U16 74HC244 U17 ULN2803 U18 74HC244 U19 74HC244 J18CON18A
PINP4 PCLKB PUT9 18 2 P60 P20 18 2 PINP1 P74 18 2 PFOTO1 RVPPE 1 18 REVPP R74 18 RFOTO1 R20 18 2 RINP1 U20 74HC244 RINP4 RCLKB
PINP5 1 2 PCLKC PUT10 1Y1 1A1 P61 P21 1Y1 1A1 PINP2 P75 1Y1 1A1 PFOTO2 PMD2E I1 O1 EPMD2 R75 1Y1 1A1 2 RFOTO2 R21 1Y1 1A1 RINP2 RUT9 R60 RINP5 1 2 RCLKC
3 4 16 1Y2 1A2 4 16 1Y2 1A2 4 16 1Y2 1A2 4 2 I2 O2 17 16 1Y2 1A2 4 16 1Y2 1A2 4 18 1Y1 1A1 2 3 4
PINP6 PCLKD P80 14 6 PINP9 P22 14 6 PINP3 P76 14 6 PFOTO3 RMD2E 3 16 ERMD2 R76 14 RFOTO3 R22 14 6 RINP3 RUT10 16 4 R61 RINP6 RCLKD
PINP7 5 6 RESP P81 1Y3 1A3 PINP10 P23 1Y3 1A3 PINP4 P77 1Y3 1A3 PFOTO4 P17 I3 O3 PDAR1 R77 1Y3 1A3 6 RFOTO4 R23 1Y3 1A3 RINP4 R80 1Y2 1A2 RINP9 RINP7 5 6 RESR
7 8 12 1Y4 1A4 8 12 1Y4 1A4 8 12 1Y4 1A4 8 4 I4 O4 15 12 1Y4 1A4 8 12 1Y4 1A4 8 14 1Y3 1A3 6 7 8
PINP8 PAN1 P82 9 11 PINP11 P24 9 11 PINP5 PB3 9 11 PADC1 R17 5 14 RDAR1 RB3 9 RADC1 R24 9 11 RINP5 R81 12 8 RINP10 RINP8 RAN1
PINP9 9 10 PINP13 P83 2Y1 2A1 PINP12 P25 2Y1 2A1 PINP6 P95 2Y1 2A1 PRDY1 PA7 I5 O5 PDAR2 R95 2Y1 2A1 11 RRDY1 R25 2Y1 2A1 RINP6 R82 1Y4 1A4 RINP11 RINP9 9 10 RINP14
11 12 7 2Y2 2A2 13 7 2Y2 2A2 13 7 2Y2 2A2 13 6 I6 O6 13 7 2Y2 2A2 13 7 2Y2 2A2 13 9 2Y1 2A1 11 11 12
PINP10 PINP14 P84 5 15 PINP13 P26 5 15 PINP7 PB5 5 15 PADC2 RA7 7 12 RDAR2 RB5 5 RADC2 R26 5 15 RINP7 R83 7 13 RINP12 RINP10 RINP13
PINP11 13 14 PINP12 PB7 2Y3 2A3 PINP14 P27 2Y3 2A3 PINP8 P94 2Y3 2A3 PRDY2 PVPPE I7 O7 PEVPP R94 2Y3 2A3 15 RRDY2 R27 2Y3 2A3 RINP8 R84 2Y2 2A2 RINP13 RINP11 13 14 RINP12
15 16 3 2Y4 2A4 17 3 2Y4 2A4 17 3 2Y4 2A4 17 8 I8 O8 11 3 2Y4 2A4 17 3 2Y4 2A4 17 5 2Y3 2A3 15 15 16
20 1 20 1 20 1 9 10 20 20 1 RB7 3 17 RINP14
C 17 18 VCC 1OE VCC 1OE VCC 1OE GND COM VM VCC 1OE 1 VCC VCC 1OE 2Y4 2A4 17 18 C
VCC 19 VCC 19 VCC 19 VCC 19 19 20 1
VCC 2OE 2OE VCC 2OE 2OE VCC AVCC 2OE VCC VCC 1OE GND
VCC 2OE 19
AVCC CX14 CX16 U21 74HC244 U22 74HC244
0.1u CX15 0.1u PG1 P62 RG1 R62 CX18 CX24 VCC VCC
18 1Y1 1A1 2 18 1Y1 1A1 2
AVCC 0.1u AVCC AVCC PG2 16 4 P63 RG2 16 4 R63 0.1u CX19 AVCC CX20 AVCC AVCC
CX23 PSCLK 14 1Y2 1A2 P64 1Y2 1A2 R64 0.1u 0.1u
1Y3 1A3 6 RSCLK 14
1Y3 1A3 6
0.1u PTTL 12 8 P65 RTTL 12 8 R65 0.1u GND_SIGNAL
4
4
4
4
4
4
4
4
U23A U23B U23C U23D PPWM 9 1Y4 1A4 1Y4 1A4 RA6 U24B U24C U24D
2Y1 2A1 11 PA6 RPWM 9
2Y1 2A1 11
PAN1 3 PAN2 5 PAN3 10 PAN4 12 PB1 7 13 PLEAK RB1 7 13 RLEAK RAN1 3 U24A RAN2 5 RAN3 10 RAN4 12
+ + + + 2Y2 2A2 2Y2 2A2 + + + +
1 P70 7 P71 8 P72 14 P73 PB4 5 15 PSLI RB4 5 15 RSLI 1 R70 7 R71 8 R72 14 R73
P13 2Y3 2A3 PINP R13 2Y3 2A3 RINP
2 - 6 - 9 - 13 - 3 2Y4 2A4 17 3 2Y4 2A4 17 2 - 6 - 9 - 13 -
C27 + LM324 C28 + LM324 C29 + LM324 C30 + LM324 20 1 20 1 C31 + LM324 C32 + LM324 C33 + LM324 C34 + LM324
VCC VCC VCC 1OE VCC VCC 1OE
2OE 19 2OE 19
11
11
11
11
11
11
11
11
1uF 1uF 1uF 1uF CX21 1uF 1uF 1uF 1uF

0.1u J11 H 3 VCC VCC
VCC RR1 10K9+C P91 R10 100KP65
PMD2 2 1 TX5V CX22
1
+
+
+
+
+
+
+
+
C25 0.1u CX25 RMD2 3 VCC ON2 X91 R13 100KR65 0.1u
C35 1uF C36 1uF C37 1uF 0.1u C38 1uF J12 RVPP 4 3 C39 1uF C40 1uF C41 1uF C42 1uF
D6 R39 6K8 RX PVPP 5 J9 CON26A J10 CON26A RR4 10K8+C
RTS BOOT RR6 10K8+C MAX202 R93IN 3ON PG1 PADC2 RG1 RADC2
U25 PB6 6 P43 2 1
R40 2 P92IN P92 RX2 2 1PFOTO1 2 PSLI 1 RFOTO1 2 RSLI
1 13 R1IN R1OUT 12 1
SELP 7
3 4 3 4
P42 3
1N4148 R41 3 RX 8 9 P93 P66 8 PG2 PLEAK RG2 RLEAK P41 4
R40 3K3 R42 4 P90 R2IN R2OUT P90OUT H3 5PFOTO2 6 PRDY2 5 RFOTO2 6 RRDY2
11 T1IN T1OUT 14 R66 9
7 8 7 8
P40 5
J13 CON10A R43 5 TX5V 10 7 TX I0 10 PFOTO3 PTTL RFOTO3 RTTL P47 6
12.5V R44 6 C17 T2IN T2OUT 9PINP 10 9 RINP 10 VM P46 7
1 2 PDAR2 11 12 PDAR1 VM 11 12 RDAR1
R45 7 1 U26 MAX202 P45 8
POSR4 3 4 POSR3 R46 8 0.1u C+ R92 VM PRDY1 13 14 PAN2 VM RRDY1 13 14 RAN2
5 6 3 C1-
R92IN 13
R1IN R1OUT 12 16 16
P44 9
D POSR2 POSR1 R47 9 4 R93IN 8 9 R93 PFOTO4 15 RFOTO4 15 D
PAN4 7 8 C18 C2+ R90 R2IN R2OUT R90OUT ALFD1 17 18 ALFD1 17 18 0 Riferimento RMP 60
9 10 5 C2- 11 T1IN T1OUT 14 19 20 19 20
R91 TX2 PPWM 12.5V RPWM 12.5V Rev. Description Checked Approved
2 V+ 10 T2IN T2OUT 7 21 22 21 22
J14 CON10A 0.1u C20 6 C19 PSCLK PADC1 RSCLK RADC1 RR5 10K8+C Approved A.G. 8-1-2001
12.5V RR7 10K8+C C21 V- PAN3 23 24 12.5V RAN3 23 24
1 2 1 C+ 25 26 25 26 12.5V
P17 2 1
RDAR2 2 1 0.1u 0.1u 3 PA73 A.G. 8-1-2001
3 4 C1- Checked
ROSR4 ROSR3 3 0.1u 4 VCC PA64
ROSR2 5 6 ROSR1 RA64 VCC C22 C2+ CX26 MICRO R MICRO P Drawn R.CORNACCHIA
7 8 5 C2- 5
RAN4 5 J16 JUMP LS1 2 0.1u VCC VCC VCC VCC VCC VCC VCC VCC P62 6 Title
9 10 R62 6 PDAR2 R44 1R 0.1u V+
2 1 6 V-
P63 7 ANALYTICAL CONTROL BOARD
D7 RESB R63 7 1 C23 C24 8
DTR R41 6K8 0.1u RA78 J15 JUMP CX27 CX28 CX29 CX30 CX1 CX2 CX3 CX4 9 Size Document Number Rev
R17 9 RDAR2 R43 1R 2 VCC 0.1u 0.1u 0.1u 0.1u 0.1u 0.1u 0.1u 0.1u 0.1u 0.1u A3
1N4148 2 1 ON [SE]00195-01 0
BUZZER
R42 3K3 C26 VCC Date: Monday, January 08,2001 Sheet 1 of 1
1 2 3 4 5 6 7 8
5 4 3 2 1
VM C19 VM C20
R10 750R 100/50 C15 0.1u R11 750R 100/50 C16 0.1u
VCC VCC
+
+
J1 J8
C3 D1 C4 D2 AM62 AM62N AM10 AM10N
0.1u 0.1u BM62 1 2 BM62N BM10 1 2 BM10N
13 VCC VMA 24 13 VCC VMA 24 3 4 3 4
D17 15 AM62 D19 15 AM74 AM74 AM74N IN8 RIS3-EV2
LED/YELLOW VMB D3 AM62N LED/YELLOW VMB D4 AM74N BM74 5 6 BM74N RIS1 5 6 RIS2
7 CK1 7 CK1 7 8 7 8
RES 2 23 BM62 RES 2 23 BM74 AM53 AM53N
CLKA RES FA1 BM62N CLKB RES FA1 BM74N BM53 9 10 BM53N VM 9 10
6 CK2 FA2 20 6 CK2 FA2 20 11 12
ENA 3 D5 ENB 3 D6 CON10A
DIRA EN DIRB EN
D 5 19 5 19 CON12A D
PDA DIR FB1 PDB DIR FB1
10 PD FB2 16 10 PD FB2 16
4 D7 4 D8
C5 M1A OSC C6 M1B OSC
8 M1 SA 21 8 M1 SA 21
3n3 M2A 9 18 3n3 M2B 9 18
M2 SB M2 SB
1 22 R1 R2 1 22 R3 R4
GNDD GNDPA 1R-2W 1R-2W GNDD GNDPA 1R-2W 1R-2W
GNDPB 17 GNDPB 17
U1 SDIC403 U2 SDIC403
VM C21 VM C22
R12 750R 100/50 C17 0.1u R13 750R 100/50 C18 0.1u
VCC VCC R16 10K R17 10K
+
+
M1A M2D
C9 D9 C10 D10
0.1u 13 24 0.1u 13 24
D18 VCC VMA AM53 D20 VCC VMA AM10
VMB 15 VMB 15
LED/YELLOW 7 D11 AM53N LED/YELLOW 7 D12 AM10N R18 10K R19 10K
RES CK1 BM53 RES CK1 BM10 M2A M1D
2 RES FA1 23 2 RES FA1 23
CLKC 6 20 BM53N CLKD 6 20 BM10N
ENC CK2 FA2 D13 END CK2 FA2 D14
3 EN 3 EN
C DIRC 5 19 DIRD 5 19 C
PDC DIR FB1 PDD DIR FB1 R20 10K R21 10K
10 PD FB2 16 10 PD FB2 16
4 D15 4 D16 M1B M2C
C11 M1C OSC C12 M1D OSC
8 M1 SA 21 8 M1 SA 21
3n3 M2C 9 18 3n3 M2D 9 18
M2 SB M2 SB
1 22 R5 R6 1 22 R7 R8 R22 10K R23 10K
GNDD GNDPA 1R-2W 1R-2W GNDD GNDPA 1R-2W 1R-2W M2B M1C
GNDPB 17 GNDPB 17
U3 SDIC403 U4 SDIC403 VCC
U6 ULN2803
UT1 1 18 LSTB-EV1
UT2 I1 O1 RIS3-EV2
2 17 SW1
UT3 I2 O2 LSTAT-EV3 M1A ON
3 I3 O3 16 1 8
UT4 4 15 LRDY-EV5 2 7 M2A OFF
UT5 I4 O4 NC-LOCK M1B ON
J4 J7 5 14 3 6
UT1 ENA WLST4-NC CLKB UT6 I5 O5 NC-MP2 M2B OFF
1 2 1 2 6 I6 O6 13 4 5
UT2 ENB WLST5-NC CLKC UT7 7 12 NC-MP3
UT3 3 4 ENC KSMP-NC 3 4 CLKD UT8 I7 O7 NC-MP4
8 11 SW DIP-4
UT4 5 6 END KSTAT-NC 5 6 RES I8 O8
7 8 7 8 9 GND COM 10
UT5 DIRA IN8 AN1
UT6 9 10 DIRB KLSTAT-NC 9 10 LIQ3-NC VM
UT7 11 12 DIRC KLRDY-OSM0A 11 12 LIQ2-NC
UT8 13 14 DIRD KLSTB-OSM0B 13 14 LIQ1-NC
B 15 16 15 16 B
UT9 PDA U7 ULN2803
UT10 17 18 PDB 17 18 UT9 RIS1
19 20 1 I1 O1 18
WLST1-LK PDC CON18A UT10 2 17 RIS2
WLST2-IL 21 22 PDD IN1 I2 O2 OUT1
3 16 SW2
WLST3-NC 23 24 CLKA IN2 I3 O3 OUT2 M1C ON
25 26 4 I4 O4 15 1 8
IN3 5 14 OUT3 2 7 M2C OFF
CON26A IN4 I5 O5 OUT4 M1D ON
6 I6 O6 13 3 6
IN5 7 12 OUT5 4 5 M2D OFF
IN6 I7 O7 OUT6
8 I8 O8 11
9 10 SW DIP-4
GND COM
VM
D21
LED/GREEN
J3 VM
J2 GND R14
KLRDY-OSM0A WLST3-NC 6 J6
1 2 5 1K U5
KLSTB-OSM0B WLST2-IL LM7805/TO J5
KLSTAT-NC 3 4 WLST1-LK PWS 4 12.5V VCC IN6 7 OUT6
5 6 3 1 VIN VOUT 2 6 6
LSTB-EV1 NC-LOCK IN5 OUT5
LIQ1-NC 7 8 LIQ2-NC 2 24V VM 5 IN4 5 OUT4
9 10 NC-MP4 1 + C13 + C14 4 IN3 4 OUT3 Riferimento RMP60
SPARE
A 0 A
GND
VM KSMP-NC 11 12 LIQ3-NC R15 100uF 10uF 3 IN2 3 OUT2
SPARE
LSTAT-EV3 13 14 NC-MP3 6PIN 2 IN1 2 OUT1 Rev. Description Checked Approved

15 16 2K2 1 1
3
KSTAT-NC VM Approved A.G. 09-01-2001
LRDY-EV5 17 18 NC-MP2
WLST5-NC 19 20 AN1 CON6 CON7 A.G. 09-01-2001
21 22 Checked
WLST4-NC VM D22
23 24 Drawn R.CORNACCHIA
25 26 LED/GREEN Title
CON26A STEPPER MOTORS DRIVER BOARD
Size Document Number Rev
A3
[SE]00188-01 0
Date: Tuesday, January 09,2001 Sheet 1 of 1
5 4 3 2 1
5 4 3 2 1
J2 PDIL EXT.ARM J5
U4 J1
G1 1 2 PDIL VM +12.5V LM7805/TO220 AM6
1 2 AM6 AM6N 1
OS5 3 4 SLI J8 D6 R18 2K2 1 2 VCC 1 2 AM6N
3 4 VIN VOUT BM6 BM6N 2
G2 5 6 LEAKS 3 4 BM6
5 6 2 AM7 AM7N 3
OS6 7 8 5 6 BM6N
7 8 1 BM7 BM7N 4
OS7 9 10 OFFPR LED + C5 + C4 7 8
GND
9 10 AM5 AM5N 5
ALFD3 11 12 VM 76384-302 100uF 10uF 9 10
11 12 BM5 BM5N 6
13 14 PDIL VCC 11 12
VM 13 14 7
3
15 16 P71
D 15 16 MICS-D 12 8 D
OS1 17 18 R13
ALFD1 17 18 76384-308
19 19 20 20
PWMR 21 22 +12.5V 50K
8
21 22 R15 10K U2B
23 23 24 24
P72 25 26 +12.5V NTC+ 5 INT.ARM J4 VERT.ARM J3 DILUTER J6
25 26 + AM5
7 P72 AM7 AM1
1 AM5N 1 1
6 AM7N J15 AM1N
- +12.5V VM 2 BM5 2 2
MICS-D 26 LM358 BM7 AM1 AM1N BM1
3 1 2 BM5N 3 3
+12.5V R14 R16 BM7N BM1 BM1N BM1N
4 3 4 4 4
4
VERT. 33K R33 R34 IN8 RIS3
R? 5 5 6 5 5
OS5 10K 0R RIS1 RIS2
4 6 7 8 6 6
J9
3 VPRE 7 9 10 7 7
2 J7 8 8 8
R17 10K MICS-D 10
1 R19 2.4K R20 10K IN8 76384-308 76384-308 76384-308
76384-304 8 RES2 C7 100uF J17 LS1
7 RES1 R31 VM 1 1
6
+
D7 4.7V NTC+ 10K 2 12 RES1
5 +12.5V 2
3 3
VPRE 11
EXT.ARM 4 VCC RL
4 4 14
3 C1 +
PRE/HEATER
C OS6 VM 5 RL RES2 R60 220R C
HOME SENSOR
22 3
2
4 2 100uF 5
SLI 6 PWRES21 OFFPR 2 Q6
3 1 6
J10 + C6 D12 24 1
2 76384-308 D11 100uF R59 2N2907
1 1
R21 2.4K R22 10K CON6MX BAY72 2 10K
76384-304 LED
1
ALFD3 VCC
D8 4.7V VCC 5V BIP.8A
INT.ARM R5
R23 220 R24 240 R32 750 C2 750
4 OS7 Q4 Q5
2
J11 3 2N2907 2N2907
2 D1 R1 R4 0.1 MOT+ 3 VCC VCC
1 1 MOT- 3 1
R25 2.4K R26 10K D5 V1
76384-304 BAY72 10K R53 R57
1M
ALFD1 J12 U1A LED 10K 10K R58 2R2
1
2
2
D9 4.7V 3
2 +
LEAK
1 LEAKS R52 R56 J14
DILUTER 1 V1 220R V1 220R SLI
2 - 1
B R27 330 R28 240 76384-302 LM358
HOME SENSOR
B
2
4 OS1 R50 MOT- R55 MOT+ VM 2
3 3
4
J13 D2 R2 R3 10K 10K
2 MOT- 4
1 3 3 5
R29 2.4K R30 10K BAY72 10K G1 2 Q2 G2OUT 2 Q3 MOT+
6
2M2
76384-304 1 1
1
R51 220R 2N2222A R54 220R 2N2222A 76384-306
D10 4.7V V1 V1
12V=1K R12 2K2 24V=2K2 J16

VPRE C3 RIS1
1 NOT USED
VCC RIS2
D4 2
G1
G2
RIS3
D3 0.1 3
8
LED R11 10K VM 4
1N5819 V1 U2A R61 R62 5
3 +
PWRES 1 P71 10K 10K 76384-305 0 Riferimento RMP 60
2 - U5C Rev. Description Checked Approved
9
LM358 8 G2OUT Approved A.G. 11-01-2001
4
A
R7 22R Q1 G2X 10 A
PWMR STP16NE06L Checked R.C. 11-01-2001
R10 U5A 74LS00 R.CORNACCHIA
R6 Drawn
G1 1 U5B
10K Title
R8 R9 3 4
R49 10K 2 6 G2X SAMPLING INTERFACE ASSY
1R/2W 1R/2W G2 5 Size Document Number Rev
10K 74LS00
A4
74LS00 [MA]00192-01 A
Date: Wendnesday, January 10, 2001 Sheet 1 of 1
5 4 3 2 1
5 4 3 2 1
J12
SCLK(J1/23) 10 9 DRDY1(J1/15) U4 TC7660
J1 ADC1 ADC1(J1/24) 8 10 9 +12.5V
8 7 7
G1 1 2 ADC2 SAMP. V- 6 5 G2(J1/5) 6 5 V-
OS2 1 2 6 5 G1(J1/1) C6 + LV VOUT
3 3 4 4 4 4 3 3
G2 5 6 LEAKP +12.5V 2 1 1uF 2
OS3 5 6 DRDY2 2 1 R16 CAP+ C7
7 7 8 8 4 CAP- +
OS4 9 10 OFFPL + MICS-D 10 100 7 100uF
ALFD3 9 10 + VM C8 OSC
11 11 12 12
D + VM 13 14 BCU 100uF 8 D
DRDY1 13 14 P71 V+
15 15 16 16 3 GND
OSB J13
17 17 18 18
ALFD1 19 20 SCLK(J1/23) 10 9 DRDY2(J1/8) D5 + C4
PWMP 19 20 +12.5V ADC2 10 9 R33 R? 10uF
21 21 22 22 ADC2(J1/2) 8
8 7 7
SCLK 23 24 ADC1 REF. V- 6 5 9.1V 1W
P72 23 24 +12.5V 6 5 + VCC
25 25 26 26 4 4 3 3
+12.5V 2 1 U5
2 1 R34 R? +12.5V LM7805/TO
MICS-D 26 MICS-D 10 1 2 + VCC
BCU(J1/14) J14 VIN VOUT
+ VM
J6 D6 R20 2K2 + C5
1
GND
CUVETTE + VM 1 10uF
2 2 2
HOLDER 3 + C1
1 3
3
SOLENOYD LED YELLOW 4 220uF
CON2P 4
J11 + VM CON4MX
+ VCC
C WASH +12.5V SPARE 2 R10 2K2 C
1 R15 + VM
STATION
OS3(J1/7) CON2P
4 50K D4
8
J3 3 J8 R11 10K U2B D3
2 REACTION LED YELLOW
1 2 5 +
R27 2.4K R28 10K TRAY TEMP. 7 P72(J1/25) 1N5819
CON4P 1 PWRES(J10/1)
SENS. 6 -
CON2P LM358
D9 4.7V R12 R14 C3
4
FILTERS J9 33K + VCC
WHEEL 10K R9 22R Q1
HOME SENSOR
OS2(J1/3) LO/HI 5 PWMP(J1/21) STP16NE06L 0.1
8
4 4 OFFPL(J1/10) R8 10K U2A

LAMP
J2 3 3 R13 10K
2 ENERGY 2 3 +
+12.5V R19 1 P71(J1/16)
1 LEVEL 1
R25 2.4K R26 10K 10K 2 -
CON4P CON5P
ALFD3(J1/11) LM358
4
D10 4.7V J10 R17 R7

B RTC + VM 1R/2W 1R/2W B
TRAY R29 220 R30 240 REACTION 2 R5
OS4(J1/9) 2 PWRES + VCC
TRAY 1
4 1
3 HEATER
J4 R18 R6 10K
2 CON2MX C2 750R
1 R23 2.4K R24 10K 10K
2
CON4P
ALFD1(J1/19) D1 R1 R4 0.1
D11 4.7V D7
CUVETTE BAY72 1M 10K U1A
8
R31 330 R32 240 J7 LED RED
HOME SENSOR
HOLDER
1
OSB(J1/17) 3
4 REACTION 2 +
1 LEAKP(J1/6)
J5 3 1
TRAY LEAK 2 -
2 CON2P LM358
2
1 R22 2.4K R21 10K Riferimento RPM 60
0
4
CON4P D2 R2 R3 Rev. Description Checked Approved

D8 4.7V BAY72 10K Approved A.G. 09-01-2001
2M2
A A
1
Checked A.G. 09-01-2001
Drawn R.CORNACCHIA
VM = 24V Title
VCC = 5V REACTION TRAY INTERFACE
A4 [MA]00193-01 0
Date: Tuesday, January 09,2001 Sheet 1 of 1
5 4 3 2 1
5 4 3 2 1
D D
J2 J1
AM62 AM62N
MOTOR STEPPER BLUE/ 8 8 BM62 1 2 BM62N
7 7 AM74 3 4 AM74N
6 WHITE 6 6 5 6
BM74 BM74N
C
5 5 BM62N AM53 7 8 AM53N C
5 BLACK 4 4 9 10
BM62 BM53 BM53N
3 3 AM62N 11 12
4 BLUE 2 2 AM62 MICS-D 12
1 1
FILTER WHEEL MOTOR
1
2
3
RED/WHITE
CON8 76384-308
WHITE
RED J3
MOTOR STEPPER 8 8
7 7
6 ORANGE 6 6
5 5 BM74N
5 WHITE 4 4 BM74
3 3 AM74N
4 BLUE 2 2 AM74
1 1
WASHING STATION MOTOR
1
2
3
RED
B CON8 76384-308 B
BLACK
YELLOW J4
MOTOR STEPPER 8 8
7 7
6 YELLOW 6 6
5 5 BM53N
5 BLACK 4 4 BM53
3 3 AM53N
4 RED 2 2 AM53
1 1
REACTION TRAY MOTOR
1
2
3
BLUE
CON8 76384-308
WHITE
0 Emission
ORANGE Rev. Description Checked Approved
Approved A.G. 30-05-2001
A A
Checked A.G. 30-05-2001
Drawn R.CORNACCHIA
Title
REACTION CHAMBER MOTOR INTERFACE
A4 [MA]00341-00 0
Date: Wednesday, May 30,2001 Sheet 1 of 1
5 4 3 2 1
8 7 6 5 4 3 2 1
R6
NOTA : 1
D Per valore di R1 vedi la relativa soluzione 0805 D
TO
+ VS
CASE
C1
.1uF R1 ? M U1 R7 R8
0805 1/4W R2 10K 10K
5.7K 0805 0805
C2 100pF 0805 +12,5 V 8 1 + VS
VOUT
2-3-6-7
TR1 C3 GND C4
5K .1uF MC78L05ACD C5
3296X SOP8 .1uF
0805 0805 10uF
7
5
Photodiode FD1 R3 JP1
2 U2 5.7K U3
- 6 0805 1 16 1 2 +12,5 V
C +VIN DGND 1 2 C
3 + 2 15 G1 3 4
OPA111 -VIN G1 G0 3 4 -V
3 AGND G0 14 5 5 6 6
TO99 U4 + VS 4 13 7 8
VS CS 7 8
4
8
1
+ VD 5 12 DRDY 9 10
TR2 C16 VREF DRDY 9 10
6 DSYNC CLK 11
8 1 7 10 SCLK
.1uF VOUT VD SCLK
2-3-6-7 8 9 DOUT
U5 0805 GND C6 DGND DOUT
100K +12,5 V C7
MC79L05ACD 3296X MC78L05ACD .1uF ADS1250
-V 2 1 SOP8 10uF 0805 SOL16
IN OUT + VS
3 IN C9 R9 R10 R11
6 C10
C8 IN 10K 10K
7 IN
-VS 10uF 10K
.1uF .1uF C11 + VD 0805 0805 0805
0805 5 0805 .1uF + VD Y1
COM 0805 1 OSC-HS 4
SOP8 2 3
7
1
R5
+ VS 3 U6 OSC4P
+ C18 C17
Z1 6
R4 5.7K 10K C12 C13 15pF .1uF
B 2 B
0805 .1uF - C14 C15 0805 0805
0805 OPA350
10uF 0805 .1uF
SO8
4
5
10uF 0805
LM4040
SOT-23
0 Emission
Rev. Description Checked Approved
Approved
A.G. 25-05-2002
A A
Checked L.M. 25-05-2002
Drawn R.CORNACCHIA
Title
PRE-AMPL/ADC
A4 [MA]00107-00 0
Date: Friday, February 06, 2004 Sheet 1 of 1
8 7 6 5 4 3 2 1
5 4 3 2 1
D D
INIT-L
D1
1N4002
U1 M1 INSTALLED ON
C P1 LT1083CP C
PHOTOMETER
1 1 1 VI VO 3
2 R3 1K 1
2 ADJ
3 3 OFFPL
4 4
5 5
R1
120R
R2 0R
TO REACTION CHAMBER + C1 + C2
C Q1
INTERFACE BOARD 100uF/25V 100uF/25V
B
P1 E
+ C3 2N3904 R4
1K
10uF/25V 1K M2
2
HALOGEN LAMP
B B
6V/10W
0 Emission
A A
Checked L.M. 12-01-2002
Drawn R.CORNACCHIA
Title
PHOTOMETER LAMP PWS
A4 9-[MA]-30-0004-00 0
5 4 3 2 1
5 4 3 2 1
D D
J1 R6 R7
R1 U1 R4 U2
WLST1 1 2 WLST2 10K 10K
3 4 1 1
WLST3
WLST5 5 6 680R WLST1 680R WLST2
7 8 3 3
+ VM (24V) 9 10
11 12 2 2
WLST4 5 5
13 14 OPB848 OPB848
15 16
MICS-D 16
C C
U6 R8 R9 R10
LM7805/TO220 R5 U3 R11 U4 R12 U5
1 2 + 5V 1 10K 1 10K 1 10K
VIN VOUT
680R 3 WLST3 680R 3 WLST4 680R 3 WLST5
C1 + + C2
GND
B 2 2 2 B
100uF 100uF 5 5 5
3
OPB848 OPB848 OPB848
0 Emission
A A
Checked A.G. 12-1-2002
Drawn R.CORNACCHIA
Title
SAMPLE RACKS IDENTIF. BOARD
A4 [MA]00446-00 0
5 4 3 2 1
5 4 3 2 1
U5 J8 J9 J10 J11 J2 J3 J4 J5 J6
LM7805/TO220 READY ST-BY AUDIBLE SPARE SAMPLE SPARE RINSE D.WATER CLEANING
+24V 1 2 +5V R7 R13 R15 ALARM OFF R17 COVER BOTTLE BOTTLE SOL
V IN V OUT 750R 750R 750R 750R R21 R2 R3 R24 R5 BOTTLE
D R9 R14 R16 R18 10K 10K 10K 10K 10K D
+ C1 + C2 10K 1 10K 1 10K 1 10K 1
GND
2 2 2 2 1 1 1 1
100uF 100uF 3 3 3 3 1 2 2 2 2
4 4 4 4 2 3 3 3 3
3
76384-304 76384-304 76384-304 76384-304 76384-302 76384-303 76384-303 76384-303 76384-303
C C
J1
KLRDY WLST3
KLSTB 1 2 WLST2 R6
LS1
KLSTAT 3 4 WLST1
LSTB 5 6 LOCK LOCK 50K
1
7
LIQ1 7 8 LIQ2 J13 U6

9 10 MP4 R8 10K
+VM (24 V) 11 12 2 2 3 +
KSMP LIQ3 BUZZER NTC 6 AN1
LSTAT 13 14 MP3 1
15 16 2 -
KSTAT +VM (24 V) 76384-302
LRDY 17 18 MP2 LMC7101
19 20
4
WLST5 AN1 R10 R12

WLST4 21 22 R11
23 24
+VM (24 V)
B 33K 10K B
25 26 10K
MICS-D 26
J7
WLST1 1 2 WLST2
WLST3 3 4 J12
WLST5 5 6
7 8 5
0 Emission
9 10 4 MP3
11 12 3 Rev. Description Checked Approved
WLST4 MP2
13 14 2 Approved A.G. 04-06-2001
15 16 1
A A
MICS-D 16 76384-305 Checked A.G. 04-06-2001
Drawn R.CORNACCHIA
Title
CONTROL PANEL
A4 [MA]00342-00 0
Date: Monday, June 04,2001 Sheet 1 of 1
5 4 3 2 1
5 4 3 2 1
J1
OSM0A R17 10K
OSM0B 1 2 IL
R11 10K 3 4 LK
EV1 5 6 LOCK VM EV1 VM EV3 VM EV4 VM EV5 VM EV6
R12 10K 7 8 R18 10K
VM 9 10 MP4 2 2 2 2 2
R13 10K 11 12 R19 10K D1 D2 D3 D4 D5
D EV3 13 14 MP3 1N4002 1N4002 1N4002 1N4002 1N4002 D
R14 10K 15 16 VM
EV5 17 18 MP2 EV1 EV3 MP2 EV5 MP3
R15 10K 19 20 AN1 1 1 1 1 1
21 22 VM
R16 10K 23 24 24V100% 24V100% 24V100% 24V100% 24V100%
25 26
MICS-D 26
VM
D6
J2 VM J3 VM J4 VM J5 J6 1N4002 J8
AM0 AM0N AM0
BM0 1 2 BM0N MP2 1 1 MP4 1 AM0N 1 LOCK 1
IN8 3 4 2 MP3 2 2 BM0 2 2
5 6 76384-302 3 76384-302 BM0N 3 IL 3
7 8 76384-303 4 4
VM 9 10 5 R10 5
C
6 10K 76384-305 C
MICS-D 10
7
8 VCC
76384-308
VCC
R5
C2 750R
2
D7 R1 R4 0.1
D9
BAY72 1M 10K VM U2
7
J7 U1 LED
LM7805/TO
1
3 1 2 VCC
LEAK 2 + VIN VOUT
6 LK
1
2 -
76384-302 C3 + + C4
2
GND
B LMC7101 B
4
D8 R2 R3 100uF 100uF
3
BAY72 10K
2M2
1
VCC VCC
R6 R8
J9 330R J10 330R
A A 0 Emission
K 4 K 4
3 3 Rev. Description Checked Approved
E E
C 2 VCC C 2 VCC Approved A.G. 11-01-2002
1 R7 R20 R21 1 R9 R22 R23
A A
76384-304 76384-304 Checked A.G. 11-01-2002
10K 0R R? 10K 0R R? Drawn R.CORNACCHIA
AN1 IN8 Title
OSM0A OSM0B
HYDRAULICS INTERF. BOARD
A4 [SE]00419-00 0
Date: Monday, June 04,2001 Sheet 1 of 1
5 4 3 2 1
5 4 3 2 1
D D
VCC
C10 C11
U4 100nF U3
100nF C12 100nF
R10 R11
10K
VCC VCC 10K
RES
U2
TRG R8 THR Q R15 10K Q1
C9
10K R1 220K 1
C 100pF C
VSS TRG VSS R3 SIGNAL OUT
1K
R2 220K
R12 C2
10K
10nF
R6
270K
R16
4M7
U5
R9 PROBE
VM
2 1 VI VO 3 VCC
B 100R 1/8W B
GND
C13 + C5 + C4
2
100nF 10uF/35V 10uF/35V 3
GND
0 Emission
Approved
A A
Checked
Drawn R.CORNACCHIA
Title
Level Sensor Assy
A4 0
SE-10-01478-00
Date: 26/11/2003 Sheet 1 of 1
5 4 3 2 1
Chapter 05 – DIAGNOSTIC PROGRAM
CHAPTER 05
- DIAGNOSTIC PROGRAM -
INDEX
5 DIAGNOSTIC PROGRAM 3
5.1 STATUS BAR 7
5.2 TEST FOLDER 8
5.3 “ARM” FOLDER 9
5.3.1 VERTICAL COMMAND AREA 10
5.3.2 HORIZONTAL COMMAND AREA 10
5.3.3 STATUS COMMAND AREA 11
5.3.4 ARM SETTING COMMAND AREA 11
5.4 “DILUTER” FOLDER 12
5.4.1 OPERATIONS AREA 13
5.4.2 SENSOR STATE AREA 13
5.4.3 USER AREA 13
5.5 “OPTIC” FOLDER 14
5.5.3 FILTERS AREA 15
5.5.4 DIGITAL CONVERSION AREA 16
5.6 “PLATE” FOLDER 17
5.6.3 USER AREA 18
5.6.4 ALL MOTORS ON/OFF AREA 18
5.6.5 REACTION PLATE AREA 18

5.7 “TEMPERATURES” FOLDER 19

5.7.1 TEMPERATURES AREA 20
5.7.2 OFFSET AREA 20
5.7.3 TEMPERATURE TEST AREA 20
5.8 “WASH” FOLDER 21
5.8.2 SENSOR STATUS AREA 22
5.8.3 TEST AREA 22
5.8.5 SENSOR STATE 25
5.9 “USER” FOLDER 25
5.9.1 USER TEST MICRO 1 AREA 26
5.9.2 USER TEST MICRO 2 AREA 26
5.10 “MISCELLANEOUS” FOLDER 27
5.10.1 GENERAL RESET AREA 28
5.10.2 SENSOR CHECKING AREA 28
5.10.3 LOG RAW DATA AREA 28
5.10.4 CONTINUOUS TEST RUN 28
5.10.5 SENSOR STATUS AREA 29
5.11 “GLOBAL SETTING” FOLDER 30
5.11.1 TABLE POSITION 31
5.11.2 KEYBOARD 31
5.11.3 DOWN ARM 32
5.11.4 SETTING OF 32
5.12 “MACRO SETTING” FOLDER 34
5.13 “CONFIGURATION” FOLDER 35
5.14 “ISE” FOLDER 39

5 DIAGNOSTIC PROGRAM
The diagnostic program enables the operator to perform a complete check of each of the Liasys’
module functions.
This program has a folder structure, with each folder containing functions pertaining to the specific
module. To launch the program the operator has to click with the left side of the mouse, on the
Diagnostic area located on the lower right side of the screen of the System Monitor (Fig.1).
The Liasys must be in the stand-by state to access this area.
Fig. 1
Click Here
Once the program starts, the following screen appears (Fig. 2)
Fig. 2
Test
Folders
Optical Switch
Sensor Status
(O.S.S.S.)
Status Bar
The diagnostic program is subdivided into three distinct areas: test folders, optical switch sensor status
(O.S.S.S.), status bar. The last two areas are present on every diagnostic window.

The three areas enable the operator to verify multiple functions as specified below:
Test Folders: checks the functionality of the Liasys’ various sub-systems; The individual
functions are illustrated later on.
Optical Switch Sensor Status (O.S.S.S.): visualizes the sensor status pertaining to micro 1 that
controls the reaction plate and the cuvette wash station (Fig. 3), as well as to micro 2 that manages the
arm and the dilutor (Fig. 4)
Fig. 3
Wash Station Optical Switch

Cover Lock Switch Plate Leak Alarm
Filter Wheel’s Optical Switch
Chassis Leak Alarm
Cuvette Holder’s Optical Switch Wash Station’s Leak Alarm

Liquid Waste1’s Alarm
Reaction’s Plate’s Optical Switch Liquid Waste2’s Alarm
Furthermore, there are three types of unused fields: empty, inactive or those reserved for future use.
While the program is running, the fields given in the figure can be either of two distinct colors, red or
green.
The fields:
• Wash Station’s Optical Switch (O.S.)

• Filter Wheel’s O.S.
• Reaction Plate’s O.S.
• Cuvette Holder’s O.S.
turn green when the specific device is in the home position, and turn red when not in the home position.

The field
• Cover Lock
turns green when the cover is closed, and turns red when open.
The fields:
• Chassis Leak Alarm (Instrument Leaking)
• Wash Station Leak Alarm (Wash Station Leaking)
• Plate Leak Alarm (Reaction Plate Leaking)
turn green when there is no leaking, and red when there is.
The following two fields signal the alarms for the bottles containing the liquid waste material:
• Liquid Waste1 Alarm
• Liquid Waste2 Alarm
turning green when the liquid level is not excessive, and red when the waste bottle indicated by the
alarm is almost full.
Fig. 4
Rack Door’s Optical Switch
Arm Leak Alarm
Vertical Arm’s Optical Switch Rinse Level Alarm
Distilled Water Level Alarm

Internal Arm’s
Optical Switch ST-BY Indicator
STAT Door’s Optical Switch

Diluter’s Ready Indicator
Optical Switch Sample3 Door’s Optical Switch
STAT
External Arm’s Sample3 Door’s Optical Switch Indicator
Optical Switch Cleaning Level Alarm
Sample2 Door’s Optical Switch
Sample1 Door’s Optical Switch

The fields in the figure can be two different colors: either green or red (for the indicators in Home
Sensor, Leak, Liquid Alarm and front panel button), and either green or dark gray (for the indicators in
Rack/Door except for the field Rgt-Smp Door that can be either green or red).
The fields:
• Vertical Arm’s O.S.
• Internal Arm’s O.S.
• External Arm’s O.S.
• Diluter O.S.
turn green when the relative device is in the Home position, and turn red when the relative device is not
in the Home position.
The following fields signal the presence of the rack in the appropriate home position (reagents,
samples, standard and controls):
• Sample1 Rack’s O.S.
• Sample 5 Rack’s O.S. (STAT)
turn green when the rack is in the appropriate home position, and turn dark gray when the rack is not
present (not inserted).
The field below signals the general door opening of any of the racks:
• Rack Door’s O.S.
turns red when the door is open, and green when the door is closed.
The field below signals the Liasys’s arm leak alarm:

• Arm Leak Alarm
turns green if there is no leaking, and red if there is.

The following fields check the liquid level of the distribution/loading bottles:
• Rinse Level Alarm
• Distilled Water Level Alarm
• Cleaning Level Alarm
turning green if the liquid level is above the predetermined minimum limit, and red if the liquid
quantity is below the predetermined minimum level (the bottle is almost empty).
The following fields signal the state of the button on the Liasys’s operator panel:
• ST-BY Indicator
• STAT Indicator
• READY Indicator
turning green when the button is not pushed in, and red if it is.
5.1 STATUS BAR
Monitors the instrument’s status while the program is running (Fig.5).
Fig. 5
Firmware Rel.
STAT Status Indicator Date
Instrument Status
ST-BY Status Indicator READY Status Indicator
Start/Stop Diagnostics

5.2 TEST FOLDER
The following is a summary of each individual test folder’function.

The Liasys’s diagnostic program is subdivided into 12 folders:
Arm: checks if the arm is functioning correctly;
Diluter: checks if the diluter is functioning correctly;
Optic: checks if the optical block is functioning correctly;
Plate: checks if the reaction plate is functioning correctly;
Temperature: checks the temperature of the pre-heater and reaction plate;
Wash: checks if the wash station is functioning correctly;
User: checks if the user functions are working properly;
Miscellaneous: performs general checks;
Global Setting: the adjustment of the arm position can be checked;
Macro Setting: the operator can identify which macros have been loaded;
Configuration: Through the use of a password, only qualified technical personal can adjust
certain parameters (This file is not accessible to the Laboratory operator).
ISE: Checks if the ISE Module is functioning properly and allows the operator to do
maintenance or repair operations.

5.3 “ARM” FOLDER
Warning: the improper use of the functions described in this folder can damage the sampling probe.
The Arm File is subdivided into four areas:

♦ VERTICAL
♦ HORIZONTAL
♦ STATUS COMMAND
♦ ARM SETTING

5.3.1 VERTICAL COMMAND AREA

(5 commands)
Home: Brings the arm vertically to a home position;
Down Well: Brings the arm’s axis “z” to the height of the well;
Up: Raises the arm tenths of a millimeter as preset in the square found immediately
on the right;
Down: Lowers the arm tenths of a millimeter as pre-determined by the square found
immediately on the right;
Go Level: Brings the probe to either the reagent level, the sample or the standard and reads
the level. Before giving this command, it is necessary to set the parameter for the
probe’s maximum lowering level in the max area (mm);
5.3.2 HORIZONTAL COMMAND AREA

(7 commands)
Back to wash well: Brings the arm back to the home position;
Go Reag.: Brings the arm to the reagent position specified in the sheet menu next to the
command;
Go Sample: Brings the arm to the sample position specified in the sheet menu next to the
command;
Go Dil.: Brings the arm to the dilution position specified in the sheet menu next to the
command;
Go ISE: Brings the arm to the ISE position;
Go Disp.: Brings the arm to the dispense position;
Go Std.: Brings the arm to the standard/control position specified in the sheet menu next
to the command;

5.3.3 STATUS COMMAND AREA
Send: Visualizes the commands sent from the program to the instrument hardware;
Receive: Visualizes the answers of the instrument’s hardware to the commands sent by the
program;
Sensor State: Indicates the sensor status relative to the arm’s vertical axis, the internal arm and
the external arm.
5.3.4 ARM SETTING COMMAND AREA

(8 commands)
+RAB: Performs the clockwise rotation of the two arms (to adjust the position);
-RAB: Performs the counter-clockwise rotation of the two arms (to adjust the position);
+RB: Performs the clockwise rotation of the external arm (to adjust the position);
-RB: Performs the clockwise rotation of the external arm (to adjust the position);
Save: Saves the adjustment data;

Next: Brings the arm to the next sample, standard, dilution or reagent position
Prev.: Brings the arm to the previous sample, standard, dilution or reagent position
Reset: Completely resets the arm
In this area the boxes can be activated when the arm is positioned on a sample, a reagent, a standard or
a control:
Original step: Visualizes the number of base steps.

Offset: Visualizes the number of offset steps.

5.4 “DILUTER” FOLDER
The Diluter Folder is subdivided into four areas:

♦ OPERATIONS
♦ SENSOR STATE
♦ USER
♦ STATUS COMMAND

5.4.1 OPERATIONS AREA

(3 commands)
Home: Brings the diluter to the Home position;
Asp: Aspirates the micro-litres as predetermined in the ul. box (may be set manually
through + and – or by using the cursor located immediately above);
Disp.: Distributes the micro-litres as predetermined in the ul. box (may be set manually
through + and – or by using the cursor located immediately above);
5.4.2 SENSOR STATE AREA
In the Sensor State area the diluter sensor status is indicated.
5.4.3 USER AREA

(1 command)
Probe Wash Test: Activates and deactivates the probe wash system
Send: Visualizes the commands sent by the program to the instrument hardware;
Receive: Visualizes the instrument hardware’s response to the commands sent by the
program.

5.5 “OPTIC” FOLDER
The Optic folder is subdivided into five areas:

♦ OPERATIONS
♦ SENSOR STATUS
♦ FILTERS
♦ DIGITAL CONVERSION
♦ STATUS COMMAND


(2 commands)
Lamp ON: Turns on the Optic Lamp;
Lamp OFF: Turns off the Optic Lamp;
This area indicates the status of the wheel filters.
5.5.3 FILTERS AREA

(10 commands)
Reset : Positions the wheel filters in home position (dark);
340 nm: Positions filter n° 1 in front of the reading sensor;
405 nm: Positions filter n° 3 in front of the reading sensor;;
Spare: Positions filter n° 9 in front of the reading sensor (Optional);

5.5.4 DIGITAL CONVERSION AREA

(2 commands)
START/STOP Sample Channel Conversion: Performs an analogical/digital conversion of the

principal channel;
START/STOP Reference Channel Conversion: Performs an analogical/digital conversion of the

reference channel;
program.

5.6 “PLATE” FOLDER
The Plate Folder is subdivided into six areas:

♦ OPERATIONS
♦ SENSOR STATUS
♦ USER
♦ ALL MOTORS ON/OFF
♦ REACTION PLATE
♦ STATUS COMMAND


(3 commands)
Home Back: Performs the same movement as made previously, but in the opposite direction
To Disp.: Automatically positions the cuvette, indicated on the sheet menu aside, under the
dispensing position
To Optic: Automatically positions the cuvette, indicated on the sheet menu aside, in front of the
colorimeter.
The status of the plate sensor is indicated in this area.
5.6.3 USER AREA

(1 command)
Cuvette Holder ON/OFF: Alternately turns ON and OFF the cuvette holder.
5.6.4 ALL MOTORS ON/OFF AREA

(1 command)
ALL Motors ON/OFF: Engages/Disengages the motors to allow a manual movement of the
modules (Sampling Arm, Reaction Plate, Washing Station, Filter Wheel
and Peristaltic Pump).
5.6.5 REACTION PLATE AREA

(1 command)
Reset: Performs the reset of the reaction plate by positioning the # 1 cuvette in the
dispensing position (reaction disp.).

Receive: Visualizes the instrument hardware’s response to the commands received from
the program.
5.7 “TEMPERATURES” FOLDER
The Temperature Folder is subdivided into three areas:

Temperatures
Offset
Status Command

5.7.1 TEMPERATURES AREA

(4 commands)
Temp. Test Run: To enter in the Temperature Test folder
Plate: Performs a temperature reading in the reaction plate compartment;
Arm: Performs a temperature reading in the arm’s pre-heater.
Calib. Sensor: To carry out the calibration procedure of the thermometric probe included
in the kit P/N 55-01204-00, as illustrated in Chapter Six “Settings and
Adjustments”.
5.7.2 OFFSET AREA

(2 commands)
To correlate the thermometric probe included in the kit P/N 55-01204-00 with another reference
thermometric probe.
Service probe: The offset value that has been previously inserted and the temperature value
read by the thermometric probe appear in the closed fields.
Cursore: To enter the offset value ± 3 C°. The offset value will be zero after exiting
from the folder.
5.7.3 TEMPERATURE TEST AREA

(3 commands)

START: To perform the test check and to regulate the temperature of the preheater and of the
reaction plate by using the kit P/N 55-01204-00, as illustrated in Chapter 6 “Setting
and Adjustments”.
The number of sample cycles are predefined, whereas the reagent and sample
positions for test execution can be selected by the operator.
Service Probe: the field in which the temperature of the service probe appears.
WASH: To run a wash cycle on the cuvettes used for testing.
EXIT: To exit from the Temperature Test box and to run a wash cycle on the cuvettes if
they have been used.
Receive: Visualizes the instrument hardware’s response to the commands sent by the program.
5.8 “WASH” FOLDER

The Wash folder is subdivided into four areas:

♦ OPERATIONS
♦ SENSOR STATUS
♦ TEST
♦ STATUS COMMAND

(2 commands)
Home: Brings the washing device to the Home position;
Down: Makes the washing device go down to the washing position.
5.8.2 SENSOR STATUS AREA

In this area the sensor state of the wash station is indicated.
5.8.3 TEST AREA

(6 commands)
Test E.V. 1: Performs switching ON/OFF of the solenoid valve three times;
µP4 – Cuvette Dry: It turns on the µP4 pump and automatically turns it off after a period of four
seconds.
Peristaltic Pump: It turns on the peristaltic pump and automatically turns it off after one second.
Washing Cycle: It performs the functional check of the Washing Station through the following
steps:
• Select, in the “Cycle #” field, the number of washing cycles to be performed from 1 to 10 and
press the “Washing Cycle” key.
• The following window appears:

• Remove the Washing Station top cover fastening screw

• Remove the Washing Station top cover and the five springs located on top of the probes
• Take out from their site the four probes and put three cuvettes in position number 1,2 and 3
(See fig.1)
Fig. 1
• Put the first two probes respectively into the first and the second cuvettes, then put the other two
probes together into the third cuvette and press the “OK” key
• By referring to the window here below, push the “OK” key to start the Washing Cycle

• Check that the three cuvettes are correctly filled and emptied (Fig. 1)
• Remove the aspiration tube from the first two probes, (they are signed as “A” and “B”), then
remove only the aspiration probe from the third cuvette and start with only one Washing cycle
(Fig.2)
Fig.2
Attention: select only one washing cycle in order to avoid cuvettes overflow
• Dispensed volumes shall be: 800 µL ± 100 into the first cuvette
1200 µL ± 150 into the second cuvette
400 µL ± 50 into the third cuvette

program.
5.8.5 SENSOR STATE

- TEST
- STATUS COMMAND
5.9 “USER” FOLDER
The User File is subdivided into three areas:

♦ USER TEST MICRO 1
♦ USER TEST MICRO 2
♦ STATUS COMMAND

5.9.1 USER TEST MICRO 1 AREA

(1 command)
Main Cover Lock ON/OFF: Alternatively turns on or off the solenoid valve holder and releases the
main cover lock.
5.9.2 USER TEST MICRO 2 AREA

(3 commands)
Alarms ON/OFF: Turns on and off the STAT indicator lamp;
Ready ON/OFF: Turns on and off the READY indicator lamp;
Test BUZZER: Performs a complete operational test of the buzzer;
Send: Visualizes the commands sent by the program to the instrument

hardware;
Receive: Visualizes the instrument hardware’s response to the commands sent
by the program.

5.10 “MISCELLANEOUS” FOLDER
The Miscellaneous folder is subdivided into six areas:
- General Reset
- Sensor Checking
- Log Raw data
- Continuous Test Run
- Sensor Status
- Status Command

5.10.1 GENERAL RESET AREA

(1 command)
Reset: Performs the resetting of the general system.
5.10.2 SENSOR CHECKING AREA

(2 commands)
Plate + 1 Step: To turn the reaction plate in a counter-clockwise direction to check the home
sensor.
Plate - 1 Step: To turn the reaction plate in a clockwise direction to check the home sensor.
5.10.3 LOG RAW DATA AREA

(1 command)
Enable: To save a copy of the files for the technical diagnosis if the instrument
malfunctions. The files are memorized in the folder “Log” inside the folder
“Analyzer”.
5.10.4 CONTINUOUS TEST RUN

(1 command)
Start Test: Performs an automatic test during which the modules (Sampling Arm, Reaction
plate, Washing Station and Photometer) are moved to all the different positions.
The correct modules positioning is also automatically verified and showed on the following table:

To stop the test push on the “Exit Test” key
5.10.5 SENSOR STATUS AREA

(1 command)
Sensor Polling: To choose either Polling ON or Polling OFF. When in the Polling ON position, it
is possible to check the functionality of the optical filters in various positions.
Send: Visualizes the commands sent by the program to the instrument hardware
Receive: Visualizes the instrument hardware’s response to the commands.

5.11 “GLOBAL SETTING” FOLDER
Warning: the improper use of the functions described in this folder can damage the sampling probe.
The Global Setting Folder is subdivided into four areas:

♦ TABLE POSITION
♦ KEYBOARD
♦ DOWN ARM
♦ SETTING OF

5.11.1 TABLE POSITION

This table has five columns:
Position: Lists all the possible instrument positions (samples, reagents, standard, controls,
diluents, ISE, dispense);
Rot A&B: Gives the base steps for the complete arm (internal and external) in every
position;
Rot B: Gives the base steps only for the external arm in every position;
Offset A&B: Gives the corrective steps necessary for the arm’s adjustment (internal and
external);
Offset B: Gives the corrective steps necessary for the external arm’s adjustment.
Next to the Sample Table there are 3 commands:
Prev: Returns the arm to the previous position;

Next: Brings the arm to the successive position;
Go: Brings the arm to the first position according to the category selected in the area
“Setting of” (Sample, reagent, Std/Ctrl, Dil, Ise, Disp).
5.11.2 KEYBOARD
The various positions can be improved through the keyboard, saving the operations performed.
The areas visible in Fig.6 may be activated by the mouse or with the help of the numerical buttons on
the keyboard. The buttons are listed with their corresponding function:
Previous Saves the

CW Rotation Position settings
CCW Rotation
Next
Position
Open External
Arm
Close External
Arm Raise Probe
Exit from Setting

Operations Lower Probe
Fig.6

5.11.3 DOWN ARM

This area has the option to decide if the arm, once set in a determined position, should automatically
command the probe to go down or wait for the manual command.
5.11.4 SETTING OF
This area has a selection of location categories that are used to set the functions (Sample, reagent,
Std/Ctrl, Dil, Ise, Disp).
Setting Procedure
⇒ Choose the position category in the “Setting of” Area;
⇒ Click with the left side of the mouse on the Go area (as soon as the area is clicked on it changes
to End);
⇒ The Liasys resets the arm, and the values of the Offset column appear on the table (Fig.7);
Fig.7

⇒ The arm centering can be adjusted at the highlighted area by giving commands through the
keyboard or mouse:
8 = CW Rotation
2 = CCW Rotation
4 = Open External Arm
6 = Close External Arm
+ = Next Position
- = Previous Position
⇒ Repeat the centering for all the relevant positions;

⇒ Push the Save button to save the operations performed;
⇒ Wait for the arm to automatically reset.
NOTE: If the option Manual (default) is chosen in the Down Arm area, to make the arm go up and
down it is possible to use either the buttons pgUp and pgDn or click with the mouse on the
button with the arm figure in the Down Arm Area.
WARNING:
THE FIRST TIME THAT THE SETTING PROCEDURE IS PERFORMED, IT IS

EXTREMELY IMPORTANT TO CHOOSE THE MANUAL OPTION IN THE DOWN ARM
AREA.
BY CHOOSING THE “AUTOMATIC” OPTION THERE IS A CONSIDERABLE RISK OF

BREAKING THE PROBE DUE TO POSITIONS THAT HAVE NOT BEEN PROPERLY
CENTERED.

5.12 “MACRO SETTING” FOLDER
This folder allows the user to read the macro present in the Liasys.
To read the macro follow the below procedure:
⇒ Select the micro from which the information will be asked;
⇒ Select from the menu sheet “Read Macro” the number of relevant macro;
⇒ Click with the left side of the mouse on the Read area;
⇒ If the Macro requested exists, the information will appear in the designated area.

5.13 “CONFIGURATION” FOLDER
This folder may be accessed only by qualified technical assistant.

It is necessary for the technical assistant to insert a password to access this folder.
The Configuration Folder is subdivided into six areas:
• Gap
• Backlash Dil.
• Heater
• Diluter Type
• Cover Lock SW.
• Wash. Well level Test

Gap: defines the volume, in µL, of the air bubble that separates the Rinse (the liquid column into the
sampling arm tube) from the sampled reagent; this volume must be set in the range from 10 to 24
(Standard value 16).
For the correct setting of the gap volume it is necessary to apply the procedure of BIB (Precision
check of the Analytical plate) and BIC (Volume precision check of the Sampling line).
If BIC’s CV values should exceed the limit specified below, the GAP has to be increased by
steps of 2 µL (starting from 10 µL) until obtaining the expected BIC’s CV value
Precision check of the Analytical plate BIB

Prepare two series of 32 samples each by using a diluted solution of K2Cr2O7 (obtained from 4
gr/l concentrated solution diluted 1:100 with Rinse solution (distilled water + 1000 µL/litre of Brij))
Use the same solution as samples and reagent
Program a new method with the following parameters:
Method name: BIB
Type: End Point
Sample volume K2Cr2O7 0,04 gr/l 3 µL
Reagent volume K2Cr2O7 0,04 gr/l 300 µL
Rinse volume 20 µL
Filter: 340 nm
Incubation time: 116 sec
Calculation factor: 1000
BIB Procedure
1. Select conical cup in Options
2. Program the BIB method using the above parameters and configure a reagent rack containing it
3. Enter in the calibration screen and select RBL for BIB method.
4. Put the Rinse solution as reagent and standard (fourteenth position of the Crt/Std rack) and Start
the Calibration
5. Program 64 BIB; fill 32 conical samples cup with K2Cr2O7 0,04 gr/l
6. Load the samples racks into the Analyzer at positions 1 and 2
7. Substitute the Rinse Solution with K2Cr2O7 0,04 gr/l in the reagent rack
8. Start the Analyzer. At the end of the last sampling, move the samples racks to positions 3 and 4.

The mean of the reading obtained from each of the 32 sample series must fall within 360 ÷ 400.
The coefficient of variation (CV %) must be lower than 0.7 % for each series of 32 samples.
Precision check of the Sampling volume BIC

Prepare two series of 32 samples each by using K2Cr2O7 4 gr/l (concentrated)
Put Rinse Solution as reagent and K2Cr2O7 solution as sample.
Program a new method with the following parameters:
Method name: BIC
Type: End Point
Sample volume K2Cr2O7 4 gr/l 3 µL
Reagent volume RINSE Solution (distilled water + 1000 µL/litre of Brij) 300 µL
Filter: 340 nm
Incubation time: 116 sec
Calculation factor: 1000
BIC Procedure
1. Program the BIC method using the above parameters and configure a reagent rack
2. Enter in the Calibration screen and select RBL for BIC method.
3. Put the Rinse Solution as reagent and Standard (fourteenth position of the Crt/Std rack) and
Start the Calibration
4. Program 64 BIC ; fill 32 conical samples cup with K2Cr2O7 4 gr/l (concentrated)
5. Load the samples racks into the Analyzer at positions 1 and 2
6. Start the Analyzer. At the end of the last sampling, move the sample racks to positions 3 and 4.
The mean of the readings obtained from each of the 32 sample series must fall between 350 ÷ 450.
The coefficient of variation (CV %) must be lower than 1.6 % for each series of 32 samples
If BIC’s CV values should exceed the limit specified above, the GAP has to be increased by steps
of 2 µL (starting from 10 µL) until obtaining the expected BIC’s CV value

Backlash Dil.: Allows to recover the sampling imprecision generated by the motor when it inverts
its rotation; the setting is expressed in µL and obtained by using the following
procedure:
1. Enter in the Diagnostic Arm folder menu and make the Sampling Arm “Reset”
2. Enter in the Diluter folder, press the “HOME” button and then Push “PROBE WASH TEST”
button three times in order to fill the hydraulic sampling line
3. Remove the plastic cover of the sample racks vane and take out the samples racks
4. Enter in the ARM folder and push the “GO” button in order to move the probe to sample
position # 1
5. Put a sample cup containing distilled water (about 1ml) under the sampling probe
6. Enter in the DILUTER folder and aspirate 300 µL of distilled water from the cup
7. Remove the sample cup containing distilled water
8. Select 1 µL to dispense and press the “Disp” key repeatedly until water appears on the tip of
the probe.
9. Substract 1 from the number of times you pressed the “Disp” key. The obtained number has to
be introduced as BACKLASH value
10. Push the Save button to memorize the inserted data
11. Push the Reset button into the ARM folder
12. Close the Diagnostic program by clicking on the Diagnostic button
Heater: to adjust the preheater and reaction plate temperatures;
Diluter Type: to select the kind of diluter to be used. Each kind of diluter has a dedicated ratio
(# steps/µL);
Cover Lock SW.: to disable the automatic check of the correct closure of the Liasys cover;
Wash. Well level Test: By clicking on the “Run Test” button the system performs an automatic
verification and adjustment cycle for the liquid level in the washing well.
A number appears in the box “Level” that corresponds to the rotation time of the rinse pump in
milliseconds, that is necessary to maintain the correct liquid level in the washing well.

The functioning range is between 400 and 700 milliseconds. If the washing well is not sufficiently
filled, a message appears to warn the user to check the hydraulic line.
If the value taken during the test is different from the previous one, it is necessary to click on “Save”
in order to save the new data.
5.14 “ISE” Folder
This folder permits checks and maintenance activity on the ISE

Purge: fills the hydraulic ISE Module line
Maintenance: empties the electrodes line for their replacement
St-By Cycle: performs a prime cycle to renew the Calibrant A solution in the electrode line
Software Release: visualizes the ISE Module Firmware release
Cleaning Cycle: performs an electrode washing cycle

Chapter 06 – SETTINGS AND ADJUSTMENTS
CHAPTER 06
- SETTINGS AND ADJUSTMENTS -
INDEX
6 SETTINGS AND ADJUSTMENTS ...................................................................................4
6.1 SAMPLING ARM...............................................................................................................4
6.1.1 ALIGNMENT AND ADJUSTMENTS ..............................................................................4
6.1.2 SUBSTITUTION OF THE SAMPLING ARM ..................................................................6
6.1.3 SUBSTITUTION OF THE OPTIC SENSORS ..................................................................7
6.1.3.1 SUBSTITUTION OF THE OS7 OPTIC SENSOR.............................................................7
6.1.3.2 SUBSTITUTION OF THE OS6 AND OS5 OPTO SENSORS..........................................9
6.1.3.2a SUBSTITUTION OF THE OS6 OPTO SENSOR ............................................................10
6.1.3.2b SUBSTITUTION OF THE OS5 OPT-COUPLER.............................................................11
6.1.4 SAMPLING ARM MOTORS...........................................................................................12
6.1.4.1 SUBSTITUTION OF THE M7 MOTOR..........................................................................13
6.1.5 PRE-HEATER AND LEVEL SENSOR ...........................................................................21
6.1.5.1 SUBSTITUTION OF THE PRE-HEATER AND LEVEL SENSOR ASSEMBLY ........21
6.1.5.2 LEVEL SENSOR FUNCTIONING CHECK ...................................................................26
6.1.6 DILUTER MODULE........................................................................................................28
6.1.6.1 SUBSTITUTION OF THE DILUTER MODULE ...........................................................28
6.1.6.2 SUBSTITUTION OF THE DILUTER MICRO-PUMP ...................................................28

6.1.6.3 SUBSTITUTION OF THE DILUTER ELECTRO-VALVE............................................29
6.1.6.4 DILUTER MODULE - CHECK PROCEDURE FOR MECHANICAL FUNCTIONING
…………… .......................................................................................................................29
6.2 OPTIC ASSEMBLY .........................................................................................................31
6.2.1 SUBSTITUTION OPTIC ASSEMBLY ...........................................................................31
6.2.2 SUBSTITUTION OF THE PHOTOMETER LAMP........................................................33
6.2.3 ELECTRONIC ADJUSTMENT OF THE PHOTOMETER ............................................34
6.2.4 SUBSTITUTION OF THE OPTO SENSOR....................................................................36
6.2.5 SUBSTITUTION OF THE MOTOR ................................................................................37
6.2.6 SUBSTITUTION OF THE OPTIC FILTERS ..................................................................38
6.3 PUMP ASSEMBLY..........................................................................................................39
6.3.1 SUBSTITUTION OF THE PUMP ASSEMBLY ............................................................39
6.3.2 SUBSTITUTION OF THE PERISTALTIC PUMP MOTOR ..........................................40
6.3.2.1 FUNCTION CHECK FOR THE PERISTALTIC PUMP.................................................41
6.3.3 SUBSTITUTION OF PART(S) ........................................................................................41
6.3.4 MECHANICAL ADJUSTMENT OF THE PERISTALTIC PUMP ...............................42
6.3.4.1 HOW TO SUBSTITUTE PERISTALTIC PUMP TUBES...............................................43
6.4 REACTION PLATE .........................................................................................................44
6.4.1 REACTION PLATE - MECHANICAL ADJUSTMENT ................................................44
6.4.2 SUBSTITUTION OF THE REACTION PLATE.............................................................45
6.4.3 SUBSTITUTION OF THE OS3 OPTIC SENSOR...........................................................46
6.4.4 SUBSTITUTION OF THE M3 MOTOR..........................................................................48
6.5 WASHING STATION ASSEMBLY................................................................................50
6.5.1 SUBSTITUTION OF THE WASHING STATION ASSEMBLY ...................................50

6.5.2 SUBSTITUTION OF THE WASHING STATION OPTIC SENSOR.............................52
6.5.3 SUBSTITUTION OF THE WASHING STATION MOTOR ..........................................52
6.5.4 WASHING STATION - MECHANICAL ADJUSTMENT AND FUNCTIONAL
CHECK……………..........................................................................................................53
6.5.4.1 WASHING STATION - HORIZONTAL ALIGNMENT ................................................53
6.5.4.2 WASHING STATION - VERTICAL ALIGNMENT.......................................................54
6.5.4.3 WASHING STATION - FUNCTIONAL CHECK ...........................................................54
6.5.5 WASHING STATION - CUVETTE HOLDER ASSEMBLY..........................................55
6.5.5.1 SUBSTITUTION OF THE CUVETTE HOLDER ASSEMBLY MAGNET ...................55
6.5.5.2 SUBSTITUTION OF THE CUVETTE HOLDER ASSEMBLY OPTO SENSOR..........55
6.5.5.3 ADJUSTMENT AND FUNCTIONAL CHECK OF THE CUVETTE HOLDER
MAGNETIC TRIGGER....................................................................................................56
6.6 TEMPERATURE CHECK AND ADJUSTMENT ..........................................................58
6.6.1 REACTION CUVETTE TEMPERATURE ADJUSTMENT AND CHECK...................58
6.6.2 TEMPERATURE CHECK AND ADJUSTMENT USING KIT P/N 55-01204-00 .........59
6.6.3 REFRIGERATION ASSEMBLY TEMPERATURE CHECK.........................................68
6.7 RACKS HOLDER ALIGNMENT ....................................................................................69
6.7.1 MECHANICAL ALIGNMENT OF THE SAMPLES RACKS HOLDER.......................69
6.7.2 MECHANICAL ALIGNMENT OF THE REAGENTS RACKS HOLDER....................70

6 SETTINGS AND ADJUSTMENTS
6.1 SAMPLING ARM
6.1.1 ALIGNMENT AND ADJUSTMENTS
1. Turn on the Liasys system (instrument and computer).

2. Launch the Diagnostic program, select "Global Setting". Wait until the instrument has
completed the reset procedure.
3. Make sure that all the "home sensors" of the Sampling Arm (Vert., Int. Arm and Ext. Arm)
light up green.
4. Make sure that the probe is centered with respect to the wash well. If not, loosen the fastening
screws on the external arm and mechanically align the arm on the wash well, paying attention
to center the probe with respect to the wash well. If absolutely necessary, also the internal arm
can be adjusted - loosen only one of its fastening screws.
Fig.1
Pre-heater Guide Support
Internal Arm
Belt 6M 203 Arm Horizontal
Movement Cd. 1-35-0005-00
External Arm
Protective Cover
Cross-bar
Wash well
Reagents - Protective Samples - Protective Cover

Cover

5. Tighten the External Arm fastening screws and, if necessary, those of the Internal Arm,
centering the probe with respect to the wash well.
6. Perform a complete Arm reset in order to ascertain correct centering of the probe on the wash
well. If the centering is not exact, repeat steps 4 and 5.
7. After having mechanically centered the sampling probe on the wash well, adjust all the other
positions by using the computer's numeric keyboard as follows:
N.B.: make sure that the distance between the top edge of the wash well and the tip of the
probe is "2.0 +/- 0.5 mm"
Use the following keys to make the necessary adjustments.

• To move the arm horizontally
8"
4" 6"
2"
• <Next / + > to move forward one position
• <Prev / - > to move back one position
• <9> to move upward
• <3> to move downward
8. Select "Sample" from "Setting of" and then press the "Go" key. The arm will automatically
move itself to position #1 of the Samples rack. Adjust the positioning by using the keys
indicated for centering the probe with respect to the hole in the Samples Cover.
9. Move on to the next position by pressing the "Next/+" key and center the probe, as above.
10. Make sure that when adjusting position #16, mechanical end-of-run of the arm does not occur.
If this should happen, repeat the mechanical alignment of the sampling probe with respect to
the wash well and repeat the procedure from step 4.
11. Press "Save" to memorize the new positions' settings.

12. Select "Reagent" from "Setting of" and then press the "Go" key. The arm will automatically
move itself to position #1 of the Reagents rack.
Adjust the positioning by using the keys indicated for centering the probe with respect to the
hole in the Reagents Cover.
13. Move on to the next position by pressing the "Next/+" key and center the probe, as above.
14. Make sure that when adjusting position #33, mechanical end-of-run of the arm does not occur.
If this should happen, repeat the mechanical alignment of the sampling probe with respect to
the wash well and repeat the procedure from step 4.
15. Press "Save" to memorize the new position settings.
16. Set the positioning of Std/Ctrl and Dil, Ise, Disp. Press "Save" to memorize the new position
settings.
17. To exit the diagnostic program, press the "Diagnostic" key
18. To exit the analyzer program press “shutdown” key..
6.1.2 SUBSTITUTION OF THE SAMPLING ARM
N.B.: Make sure the instrument (Liasys) is turned off before performing this substitution
procedure.
1. Remove the sampling probe from its housing.

2. Remove the samples and reagents racks' protective covers.
3. Remove the samples rack and reagents rack.
4. Unscrew the four cross bar fastening screws. Remove the cross bar by slipping out the Pre-
heater guide support (Fig.1).
5. Remove the waste tube from the wash well.
6. Remove the teflon tube from the diluter head by unscrewing the fastening washer, paying
special attention to leakage of liquid.
7. Unscrew the four anchored screws indicated in Fig. 5, unplug the J1, J2, J6, J8, J13, J15 and
J17 connectors from the Sampling Interface assy. Disconnect the ground wire and following
the procedure provided in section 6.1.5.1 remove the Level Sensor and Pre-Heater assembly.

8. Take out the sampling arm and substitute it with the new one.
9. To remount, repeat the above steps in inverse order: from 7 to 1.
Warning: After having substituted the sampling arm, it is necessary to mechanically align
the probe with respect to the wash well and center all the other positions by following the
procedures provided in Section 6.1.1 "Alignment and Adjustments".
6.1.3 SUBSTITUTION OF THE OPTIC SENSORS
6.1.3.1 Substitution of the OS7 Optic sensor

5. Loosen the protective cover's set screw (Fig.2) and lift it.
6. Remove the fastening screws from the optic sensor support, unplug the J11 connector from
the Sampling Interface assy and take out the entire assembly (Fig.3).
7. Remove the fastening screws from the optic sensor itself and substitute it with the new one.
8. To remount, repeat above steps in inverse order: from 7 to 1.
N.B.: when remounting the optic sensor, make sure to center it with respect to the slots, as
shown in Fig. 4.
N.B.: due to the fact that it may be necessary to further adjust the positioning of the optic
sensor, screw down the cross bar and the protective cover only after having made sure that
the sampling arm functions correctly by carrying out the procedure described in Section
6.1.1

Warning: Do not tighten the protective cover's set screw as this could result in mechanical
malfunction of the sampling arm's vertical movement (Fig. 2).
Warning: After having substituted the optic sensor, it is necessary to mechanically align the
probe with respect to the wash well and center all the other positions by following the
N.B.: the following check is valid only for the OS7 optic sensor
Verification of the correct positioning of the OS7 optic sensor

Set the Samples position at #16. If it is not possible to obtain an exact centering (due to
mechanical end-of-run), move the OS7 optic sensor to the left. After having mechanically aligned
the optic sensor, center all the other positions by following the procedures provided in Section
6.1.1
Fig. 2 Fig. 3
Washing Well assembly OS7 Opto coupler assy
Cd. 9-05-0063-00 Cd. 9-10-0023-00
Protective cover set screw

Slots
Fig. 4
6.1.3.2 Substitution of the OS6 and OS5 Opto sensors
procedure.
In order to arrive at the OS6 and OS5 Opto sensors, it is necessary to remove the arm assembly
from its housing. Unplug connectors J9 and J10 from the Sampling Interface assy; disconnect the
wash well hydraulic tube and the teflon tube on the diluter. Loosen the four anchored screws. When
removing the sampling arm, be extremely careful to not damage the pre-heater.
The following steps, from 1 to 6, are to be carried out regardless of which opto sensor is being
substituted (OS6 or OS5).
2. Remove the samples and reagents racks' protective covers (Fig.1).
5. Remove the waste tube from the wash well and the teflon tube on the diluter.
6. Unscrew the four anchored screws indicated in (Fig.5), unplug the J1, J2, J15 and J17
connectors from the Sampling Interface assy (Fig.5). Disconnect the ground wire and remove
the sampling arm.

6.1.3.2 a Substitution of the OS6 Opto sensor
a) Remove the screws which fasten the opto sensor support and take out the entire assembly
(Fig.6).
b) Unplug the J10 connector from the Sampling Interface assy, remove the opto sensor fastening
screws and substitute it with the new one.
c) To remount, repeat the above steps in inverse order: from b) to a) and then from 6 to1 in
Section 6.1.3.2
malfunction of the sampling arm's vertical movement (Fig.2).
N.B.: when remounting the opto sensor, make sure to center it with respect to the slots, as
shown in Fig. 4.
Warning: After having substituted the opto sensor, it is necessary to mechanically align the
Fig. 5 Fig. 6
Anchored screws Sampling Interface Assy OS6 Opto coupler assy

Cd.30-00192-01 Cd. 9-10-0023-00

6.1.3.2 b Substitution of the OS5 Opt-coupler
a) Carry out Steps 1 through 6 as indicated in Section 6.1.3 "Substitution of the OS6 and OS5
Opto sensors.
b) Remove the screws which fasten the opto sensor support and take out the entire assembly
(Fig.7).
c) Unplug the J9 connector from the Sampling Interface assy, remove the optic sensor fastening
screws and substitute it with the new one.
d) Pay particular attention to the spacer washers placed between the opto sensor and the support
(Fig.8)
e) To remount, repeat the above steps in reverse order: from c) to a) and then from 6 to1 in
Section 6.1.3.2
Fig. 7 Fig. 8
OS5 Opto coupler assy Spacer washers

Cd. 9-10-0024-00

N.B.: make sure that the distance between the top edge of the wash well and the tip of the
probe is "2.0 +/- 0.5 mm"
Warning: After having substituted the opto sensor, it is necessary to mechanically align the
6.1.4 SAMPLING ARM MOTORS
N.B.: Make sure the instrument (Liasys) is turned off before performing this procedure.
Important: pay special attention to the Pre-heater when removing or servicing the sampling
arm.
In order to arrive at the M7, M6 and M5 motors, it is necessary to remove the sampling arm
assembly from its housing; unplug the J1, J2, J3, J4, J5, J15 and J17 connectors from the Sampling
Interface assy; disconnect the hydraulic tube from the wash well and the teflon tube on the diluter;
remove the four anchored fastening screws from the assembly.

6.1.4.1 Substitution of the M7 motor
1. Remove the sampling probe

6. Remove the teflon tube on the diluter head by unscrewing the fastening washer - pay
particular attention to leakage of liquid.
7. Unscrew the four anchored screws indicated in Fig. 3; unplug the J1, J2, J4, J15 and J17
connectors from the Sampling Interface assy; unplug the ground wire; remove the sampling
arm.
8. Loosen the fastening screw and lift the sampling arm's protective cover (Fig.4), in order to
arrive at the M7 motor.
9. Remove the M7 motor fastening screws (Fig 5).
10. Loosen the motor pulley screws (Fig.6).
11. Take out the M7 motor and substitute it with the new one.(Fig.7).
12. Lift the pulley to approximately 0.5mm from its upper limit and tighten its screws(Fig.8).
13. Push the M7 motor outward, as shown in (Fig.9), and holding it in this position, so as to
maintain the belt in tension, tighten the motor fastening screws.

Warning: After having substituted the motor, it is necessary to mechanically align the probe
with respect to the wash well and center all the other positions by following the procedures
provided in Section 6.1.1 "Alignment and Adjustments".
Pre-heater guide support
Protective cover
Cross-bar
Fig.1
Teflon tube
Fig. 2 Dilutor Module
Anchored screws
Fig. 3 Arm assembly without base and Sampling Interface Board Cd. 9-15-0020-00

Protective cover set screw
Fig. 4
M7 motor fastening screws
Fig. 5
Pulley screws
Fig. 6

M7 Motor assy Cd. 10-00399-00
Fig. 7
M7 Motor’s belt Cd. 9-35-0031-00
Fig. 8
Fig. 9

6.1.4.2 Substitution of the M6 Motor
arm.
1. Remove the sampling probe.

6. Remove the teflon tube on the diluter head by unscrewing the fastening washer (Fig.2),
paying special attention to leakage of liquid.
7. Unscrew the four anchored screws indicated in (Fig.3); unplug the J1, J2, J5, J15 and J17
arm.
8. Take the Sampling Interface assy out by unplugging the connectors and unscrewing the
fastening screws (Fig.10).
Sampling Interface assy Cd.30-00192-01
Fig.10

N.B.: In order to be able to remove the M6 motor from its support, it is necessary to first
partially disassemble the module as shown in Fig.12.
9. Unscrew the three fastening screws (Fig.11) and remove the base as shown in (Fig.12).
10. Remove the motor fastening screws and loosen its pulley screws (Fig.13).
11. Take out the M6 motor and substitute it with the new one
12. Lift the pulley to approximately 0.5mm from its upper limit and tighten its screws(Fig.13)
13. Push the motor outward, as shown in (Fig.9), and holding it in this position so as to maintain
the belt in tension, tighten the motor fastening screws.
14. Tighten the base support screws (Fig.11).
malfunction of the sampling arm's vertical movement (Fig.4).
Fig. 11 Fig. 12
M5 Motor Assy (Vertical)
Cd. 10-00399-00

Pulley screws
M6 Motor fastening screws
Fig. 13
M6 Motor Assy Cd.10-00399-00
6.1.4.3 Substitution of the M5 motor
arm.

6. Remove the teflon tube on the diluter head by unscrewing the fastening washer (Fig.2),
paying special attention to leakage of liquid.
7. Unscrew the four anchored screws indicated in (Fig.3); unplug the J1, J2, J3, J15 and J17
arm.
8. Take the Sampling Interface assy out by unplugging the connectors and unscrewing the
fastening screws (Fig.10).

N.B.: In order to be able to remove the M5 motor from its support, it is necessary to first
partially disassemble the module as shown in Fig.12.
9. Unscrew the three fastening screws (Fig.11) and remove the base as shown in (Fig.12).
10. Remove the motor fastening screws and loosen its pulley screws (Fig.14).
11. Take out the M5 motor and substitute it with the new one
12. Lift the pulley to approximately 0.5mm from its upper limit and tighten its screws (Fig.14).
13. Push the motor outward, as shown in (Fig.9), and holding it in this position so as to maintain
the belt in tension, tighten the motor fastening screws.
14. Tighten the base support screws (Fig.11).
M5 Motor fastening screws
Pulley screws
Fig. 14

6.1.5 PRE-HEATER AND LEVEL SENSOR
6.1.5.1 Substitution of the pre-heater and level sensor assembly

4. Unscrew the four cross bar fastening screws. Remove the cross bar by slipping out the rear
Pre-heater guide support (Fig.2).
6. Remove the teflon tube on the diluter head by unscrewing the fastening washer (Fig.3) pay
particular attention to leakage of liquid.
7. Unplug connector J7 from the Sampling Interface assy.
8. Lift the teflon washer and expose the half-ring; remove the half-ring (Fig.4) and (Fig.5).
9. Remove the teflon washer and the spring (Fig.6).
10. Remove the Pre-heater assembly (Fig.7) and (Fig.8), making sure that the blocking pin
remains in place.
11. Remount the new assembly, repeating the above steps in inverse order: from step 10 to step 1.

WARNING
Do the following operations only after substitution of the Level Sensor and Pre-heater assembly:
a) Remove the white wire from the teflon tube before connecting it to the diluter
(the white wire must be removed only at this stage of the procedure and not before).
Be sure of having properly connected the tube to the diluter.
b) Remove the protecting cap before positioning the sampling probe.

Note : the length of the Pre-heater supply cable from the top of the external arm to the intermediate
guide must be approximately 23 cm (Fig.2).

13. Launch the diagnostic program. Select the "Diluter" function and press the "Probe Rinse
Cycle" several times in order to fill the hydraulic circuit with liquid.
14. Make sure that no liquid leaks from the probe assembly and/or the dilutor. Make sure that
the liquid flows from the probe freely.
15. Select the "Arm" key and then "Go Sample". Set Samples position #16. Make sure that the
Pre-heater cable does not hinder the arm while it is in movement. If this situation should
occur, turn the Pre-heater cable counter-clockwise in order to optimize the assembly.
16. Press the ”Reset” key
17. To exit the diagnostic program, press the "Diagnostic" key
18. To exit the analyzer program, press the "Shutdown" key
Fig. 1 Probe Assembly Cd. 9-10-0062-01
23 cm
Intermediate cable guide guide
Front cable guide
Rear cable guide
Cross-bar
Fig.2

Teflon tube
Kit for E. V.- Diluter connection

Cd. 99-00906-00
Fastening screw
Fig.3
Metal rings for level sensor and pre-heater assy fixing

Cd. C1-01-0230-00
Fig. 4
Removal of the Metal rings
Fig. 5

Level sensor and pre-heater spring

Cd. C1-01-0236-00
Locknut for pre-heater assy

Cd. C1-01-0232-00
Fig. 6
Level sensor and pre-heater assy locking key

Cd. C1-01-0572-00
Fig. 7

6.1.5.2 Level Sensor functioning Check

2. Remove the protective cover of the samples rack.
3. Touch the sampling probe with your finger and make sure that the level sensor LED on the
Sampling Interface assy turns on approximately one second, when the probe is touched
(Fig. 9).
Fig. 8
Level Sensor and Pre-Heater assembly
Cd 9-10-0063-00
Sensor Level LED
Fig. 9 Sampling Interface assy

Cd. 30-00192-01

N.B.: during automatic functioning (routine analysis), every time the sampling probe dips
into any liquid whatsoever, the "Level sensor" LED on the Sampling Interface assy will turn
on for approximately one second. We advise that you never allow the level sensor to operate
when the solution quantity is less than 290 µl.
4. In order to more specifically verify the correct level-sensing functioning, proceed as follows:
a) Place a cup containing liquid in the first samples rack, position nr 1 (Volume ≥ 290 µl).
b) Launch the diagnostic program. Select the "Arm" function and press the "Go Sample" key in
order to allow the probe to move into the position corresponding to the sample cup.
c) In the window next to the "Go Level", enter the maximum descent of the probe (e.g.500).
d) Select "Go Level" and make sure that the probe drops down into the cup until it senses the
liquid. In the window to the right of the "Go Level" key the level reached by the probe will
appear, expressed in tenths of millimeters
e) Repeat step d) several times, each time returning to the Vertical "Home" position. Make sure
that the level indicated remains constant ≤ 10 (1 mm).
f) Repeat steps b) to e) for positions 8 and 16 of the first rack using the same sample cup. Make
sure that the difference between all the positions is ≤ 1mm.
g) Repeat steps b) to f) for samples racks three and five (Stat positions 1, 8, 14).
h) Press the "Back to Wash Well" key and then select "Go Sample" in order to allow the probe to
return to a position in the rack, which does not contain any cup.
i) Select "Go Level" and make sure that the level reached is the maximum indicated in the given
window is 9999.
j) Select the "Reset" key, remove the cups from the racks and exit the program by pressing the
"Diagnostic" key.
k) To exit the analyzer program press “shutdown” key.

6.1.6 DILUTER MODULE
6.1.6.1 Substitution of the diluter module
1. Remove the samples and reagents protective covers (Fig.1).

3. Remove the teflon tube on the diluter head by unscrewing the fastening washer
(Fig.2), paying special attention to leakage of liquid.
4. Unscrew the four anchored screws indicated in (Fig.2); unplug the J6, J8, and J13 connectors
from the Sampling Interface assy (Fig.3). Remove the diluter and substitute it with the new
one.
5. To remount, repeat the above steps in reverse order: from 4 to 1.
6. After mounting the new module, carry out the check procedure for mechanical functioning as
described in Section 6.1.6.4
6.1.6.2 Substitution of the diluter micro-pump

3. Remove the teflon tube on the diluter head by unscrewing the fastening washer (Fig.2)
paying particular attention to leakage of liquid.
from the Sampling Interface assy (Fig.3).
5. Remove the diluter module from the instrument.
6. Unscrew the two fastening screws on the micro-pump, take out the pump and substitute it
with the new one (Fig.2)
8. After mounting the new micro-pump, carry out the check procedure for mechanical
functioning as described in Section 6.1.6.4.

6.1.6.3 Substitution of the diluter Electro-valve

3. Remove the teflon tube on the diluter head by unscrewing the fastening washer (Fig.2)
paying particular attention to leakage of liquid.
from the Sampling Interface assy (Fig.3).
5. Remove the diluter module from the instrument.
6. Unscrew the two fastening screws on the electro-valve, take it out and substitute it with the
new one (Fig.2)
8. After mounting the new electro valve, carry out the check procedure for mechanical
functioning as described in Section 6.1.6.4.
6.1.6.4 Diluter module - Check procedure for mechanical functioning

2. Remove the protective cover of the samples and of the reagents (Fig.1).
3. Launch the Diagnostic program, select the "Diluter" function" and click on "Home" field.
Make sure that the "Home Sensor" Dil. shows green.
4. Select aspiration and then dispense volumes between 3 to 500 microliters clicking on the
appropriate windows.
5. Make sure that the diluter both aspirates and dispenses as requested and that there is no
leakage of liquid from the hydraulic connections.
6. Exit the Diagnostic program by pressing the "Diagnostic" key.
7. Exit the analyzer program press “shutdown” key.

Reagents protective cover
Samples protective cover
Fig.1
Micro-pump assembly Cd. 9-10-0028-01
Diluter head Teflon fitting assy

Cd. C1-01-0042-01
Diluter 1000 µ Cd. 10-00496-00
Solenoid valve-2 way Cd. F-35-0019-00
Fig. 2 Diluter Module Anchored screw
Sampling Interface assy

Cd. 30-00192-01
Fig. 3

6.2 OPTIC ASSEMBLY
6.2.1 SUBSTITUTION OPTIC ASSEMBLY
¾ N.B.: Make sure the instrument (Liasys) is turned off before performing this
substitution procedure.
1. Remove the cover panel of the reaction plate (Fig.1).

2. Unscrew the four cross-bar fastening screws and remove the cross-bar (Fig.1).
3. Unscrew the 4 fastening screws on the reaction plate assembly (Fig.2).
4. Remove the J1, J2 connectors and the ground wire from the Reaction Chamber Motor
Interface board (Fig.2).
5. Remove the J1, J2, J14 connectors and the ground wire from the Reaction Plate Interface
board (Fig.2).
6. Open the peristaltic pump assembly panel cover. Unscrew the two fastening screws to make
free the support that join the seven tubes coming from the washing station probes.
7. Disconnect the six tubes coming from the washing station probes and the air tube inserted on
the micro pump P4 coming from the porous pad on fifth probe of the washing station.
8. Remove the reaction plate from the instrument.
9. Remove the lamp's power supply wires connected to the Lamp Regulator board by loosening
the two screws on the M1 connector (Fig. 4).
10. Remove the J1 connectors from the Preamp/ADC (Fig.5) and the ground wire from the optic
group.
11. Unscrew the four fastening screws, remove the optic assembly and substitute it with the new
one (Fig. 3).
12. Remount the assembly, repeating the above steps, 11 through 1, in reverse order.
13. After remounting, reseat the lamp and carry out the electronic adjustment check procedures as
described in Sections 6.2.2 and 6.2.3

Reaction Plate cover panel
Cross-bar
Fig. 1
Reaction Tray Interface Cd. 30-00193-01
Anchored screw
Fig. 2 Reaction Plate assembly Cd. 9-10-0061-01 *

*Reaction Plate assembly is supplied without Washing station and Photometer
Optic assembly fastening screw
Motor fastening screw
Fig. 3 New photometer assy (2optic Channel)

Cd. 10-00108-00

6.2.2 SUBSTITUTION OF THE PHOTOMETER LAMP
¾ N.B.: Make sure the instrument (Liasys) is turned off before performing this substitution
procedure.

2. Remove the lamp's electrical wires connected to the Lamp Regulator board by loosening the
two screws on the M1 connector (Fig.4).
3. Unscrew the lamp's fastening screws and remove it from its housing.
4. Remount the new lamp, repeating the above steps, 3 through 1, in reverse order.
5. Carry out the electronic adjustment of the photometer as described in Section 6D.2.1.
Warning: Do not touch the glass portions of the lamp.
Halogen Lamp Cd. 9-35-0016-00
Lamp's fastening screw
M1 Connector
Fig. 4 Trimmer P1
Lamp PWS board Cd. 9-30-0004-00

6.2.3 ELECTRONIC ADJUSTMENT OF THE PHOTOMETER
Warning: make sure that the photometer lamp and the reaction cuvettes are brand-new before
applying the following procedure.
1. Turn on the "Liasys" system /first the instrument and then the computer). Remove the reaction
plate cover (Fig.1).
2. Launch the diagnostic program, select the "Plate" function and reset by clicking on the
appropriate key.
3. Make sure that the "Home Sensor" Reaction Plate window lights up green.
4. Place 500 µL of distilled water in cuvette #31 of the reaction plate.
5. Select the "Optic" function and turn the lamp "On" by clicking on the appropriate field.
6. Reset the filter wheel by clicking on the appropriate key.
7. Make sure that "Home Sensor" Filter Wheel window lights up green.
8. Make sure that the Regulator board for Connector M1 shows 6.0 volts ± 0.1. If not, turn the
P1 trimmer (Fig.4).
N.B.: The reaction cuvette must be perfectly clean. If not, carry out a "Cuvette Wash" cycle
(Start Work)
Warning: Room lighting and daylight can influence the reading. To avoid this interference,
replace the reaction plate cover panel before carrying out any check procedures.
9. Click on "Start Sample Channel Conversion" key and make sure that the adjacent window
shows a count value of 100 ± 50. If not, adjust the Preamp/ADC board "Offset" trimmer
inside the analysis plate in order to set the desired value (Fig. 5).
10. After regulating of the main channel offset, it is necessary to click on " Stop Sample Channel
Conversion" key.
11. Click on "Start Reference Channel Conversion" and make sure that the adjacent window
shows a count value of 100 ± 50. If not, adjust the Preamp/ADC board "Offset" trimmer of
the reference channel located external to the analysis plate in order to set the desired value
(Fig. 5).
12. After regulating of the reference channel offset, it is necessary to click on " Stop Reference
Channel Conversion" key.
13. Select the filter positions from 1 to 8 (from 340 nm to 620 nm) and click on” Start/Stop
Sample Channel Conversion" key and determine which filter transmit the highest signal.
14. Verify in the adjacent window a count value of 55.000 ± 60.000. If not, adjust the
Preamp/ADC board "Gain" trimmer inside the analysis plate in order to set the desired value
(Fig. 5).
15. After regulating the Gain, select all the other filters and clicking on "Start/Stop Sample
Channel Conversion" verify count values between 29.000 and 60.000
Warning: Room lighting and daylight can influence the reading. To avoid this interference,
replace the reaction plate cover panel before carrying out any check procedures.
16. Select the highest signal filter, identified in point 13. Click on "Start Reference Channel
Conversion" and make sure that the adjacent window shows a count value of 38.000 ± 42.000.
If not, adjust the Preamp/ADC board "Gain" trimmer external to the analysis plate in order to
set the desired value (Fig. 5).
17. After regulating the Gain, select all the other filters and clicking on "Start/Stop Reference
Channel Conversion" verify count values between 29000 and 42000.
18. After concluding these operations, empty cuvette #31 and exit the diagnostic program by
clicking on the "Diagnostic" key.
19. Exit the analyzer program press “shutdown” key.
Fig. 5 New photometer assy (2optic Channel)

Cd. 10-00108-00 Pre-Amplifier/ADC (Read Channel)
Cd. 30-00107-00
Motor support
Opto coupler assy

Cd. 9-10-0023-00
Gain - Reference Channel
Stepper Motor assembly Pre-Amplifier/ADC (Ref. Channel)

Cd. 9-10-0019-01 Cd. 30-00107-03

6.2.4 SUBSTITUTION OF THE OPTO SENSOR
procedure.

4. Remove the J1, J2 connectors and the ground wire from the Reaction Chamber Motor Interface
board (Fig.2).
5. Remove the J1, J2, J14 connectors and the ground wire from the Reaction Plate Interface board
(Fig.2).
6. Open the peristaltic pump assembly panel cover. Unscrew the two fastening screws to make free
the support that join the six tubes coming from the washing station probes.
7. Disconnect the seven tubes coming from the washing station probes and the air tube inserted on
the micro pump P4 coming from the porous pad on fifth probe of the washing station.
11. Unscrew the four fastening screws on the motor support in order to access the filter wheel.
12. Position the filter wheel in such a manner as to make it possible to see and unscrew the internal
opto sensor support screw. Unscrew the external opto sensor support screw.
13. Unscrew the optic sensor fastening screws from its support and substitute with the new one.
15. After remounting, reseat the lamp and carry out the electronic adjustment procedures as
described in Sections 6.2.3

6.2.5 SUBSTITUTION OF THE MOTOR
procedure.

4. Remove the J1, J2 connectors and the ground wire from the Reaction Chamber Motor
Interface board (Fig.2).
5. Remove the J1, J14 connectors and the ground wire from the Reaction Plate Interface board
(Fig.2).
free the support that join the six tubes coming from the washing station probes. Disconnect
the seven tubes coming from the washing station probes and the air tube inserted on the micro
pump P4 coming from the porous pad on fifth probe of the washing station.
10. Unscrew the four fastening screws on the motor support in order to access the pulley.
11. Unscrew the four fastening screws on the motor; loosen the pulley fastening screws (Fig.3).
12. Remove the motor and substitute it with the new one.
Warning: when tightening the motor pulley fastening screws, make sure that the filter wheel is
centered with respect to the opto sensor.
described in Sections 6.2.2 and 6.2.3

6.2.6 SUBSTITUTION OF THE OPTIC FILTERS

Interferential filters Kit Cd. 9-65-0029-00
procedure.

4. Remove the J1 connector and the ground wire from the Reaction Chamber Motor Interface
board (Fig.2).
5. Remove the J1, J14 connectors and the ground wire from the Reaction Plate Interface board
(Fig.2).
free the support that join the six tubes coming from the washing station probes. Disconnect
the seven tubes coming from the washing station probes and the air tube inserted on the micro
pump P4 coming from the porous pad on fifth probe of the washing station.
10. Unscrew the four fastening screws on the motor support in order to access the filter wheel.
11. Unscrew the fastening screw that holds the elastic band that holds the filters.
12. Remove the filter from its housing and substitute it with the new one.
described in Sections 6.2.3.
Warning: Do not touch the glass parts of the filter.
Warning: when substituting an interferential filter due to malfunctioning or functional

anomalies, it is always advisable to substitute the entire set of filters mounted on the
instrument.

6.3 PUMP ASSEMBLY
6.3.1 SUBSTITUTION OF THE PUMP ASSEMBLY
procedure.
1. Unscrew the two knobs to remove the door located on the front elevation of the instrument
(Fig. 1) and the two noticeable screws located on the rear panel (Fig. 2) to access the washing
pump assembly.
Fig. 1
Micro-Pump (µP4) Assy

Cd. 05-00403-00 Pumps Assembly Front side
2. Disconnect connectors J1, J2 e J8 from the Hydraulics Interface board (rear side Fig.2)
3. Disconnect the air tubes from µP4 and the waste tubes coming from the peristaltic pump
(front side, Fig. 1)
4. Disconnect the three tubes inserted on the EV1, EV4 ed EV6 valves, respectively coming from
the washing well, from the distilled water bottle and from the cleaning solution bottle (front
side, Fig. 1)
5. Unscrew the four screws which hold the Pumps Assembly. Remove it from its site (rear side
Fig.2) and replace the Assembly with the new one.
6. Remount, repeating the above steps, 5 through 1, in reverse order.

Hydraulic interface board Pumps Assembly Rear side

Cd. 30-00419-00
J8 J1-J2
Fig. 2
Micro-Pump (µP3) Assy Micro-Pump (µP2) Assy Peristaltic Pump Assy

Cd. 05-00447-00 Cd. 05-00404-00 Cd. 10-00286-01
6.3.2 SUBSTITUTION OF THE PERISTALTIC PUMP MOTOR
procedure.
1. Unscrew the two knobs to remove the door located on the front elevation of the instrument (Fig.
1) and the two noticeable screws located on the rear panel (Fig. 2) to access the washing pump
assembly.
2. Unlock the hinged tube guide by lowering and rotating the guide lock to the left in order to free
the tubes (Fig. 3).
3. Disconnect the connector J6 from the Hydraulics Interface Board (Rear side Fig.2)
4. Unscrew the two motor fastening screws and remove the peristaltic pump assembly from its site
(Front side Fig.3)
5. Unscrew the four screws which hold the motor and loosen the two grains which hold the
peristaltic wheel.

Motor fastening screws
Hinged guide tube
Peristaltic Pump Rotor

Cd. 99-00902-00
Fig. 3 Hinged guide lock
6.3.2.1 Function check for the peristaltic pump
1. Turn on the Liasys system and launch the “Analyzer” program. Select "Start Work" and perform
one or more cuvette washing cycles. Make sure that the sampling probe washing well empties
completely without any leakage of liquid. If leakage should occur, adjust the screw indicated in
Fig. 5 using the Peristaltic Pump Setting Gage as illustrated in figure 6 (See Service News N° 9
dated 21.05.2003)
2. Close the "Liasys" program by clicking on "Shutdown".
6.3.3 SUBSTITUTION OF PART(S)
1. Unscrew the two knobs to remove the door located on the front elevation of the instrument (Fig.
1) and the two noticeable screws located on the rear panel (Fig. 2) to access the washing pump
assembly.
2. For each part to be replaced, if necessary, disconnect the relative electrical power connection, the
hydraulic tubes and the fastening screws.
3. If necessary, unscrew the four anchored screws of the assembly and turn it ahead (Fig.2).

Any necessary adjustments (in order to optimize pump aspiration in relation to the newly
substituted tube) can be made by turning the relative screw (Fig. 5) using the Peristaltic Pump
Setting Gage as illustrated in figure 6 (See Service News N° 9 dated 21.05.2003)
N.B:. If power loss is experienced in the filling or emptying of liquid (after a period of
instrument activity), perform the following operations before substituting or servicing any
part(s):
• Disconnect the liquid input tube from the malfunctioning pump.
• Insert a small amount of liquid using a syringe from the disconnected ends in order to wet
the membranes (Fig. 4).
Remove the syringe and reconnect the tube(s); perform functioning check as described in
Section 6.3.2.1.
Fig. 4
6.3.4 MECHANICAL ADJUSTMENT OF THE PERISTALTIC PUMP
procedure.
The peristaltic pump tubes must be substituted every three to six months, depending on instrument
use.

6.3.4.1 How to substitute peristaltic pump tubes
1. Unscrew the two knobs on the cover, located on the instrument 'headboard' behind the washing
station, in order to arrive at the pumps assembly (Fig. 1).
2. Unhook the hinged guide by lowering and rotating the guide's blocking bracket to the left
thereby freeing the tubes (Fig.3).
3. Pull the tubes off their relative nipples and substitute with new ones.
• Always use original replacement parts; never lubricate the peristaltic pump roller bearings.
• After prolonged instrument inactivity: verify the efficiency of the peristaltic pump tubes.
Please note: if the instrument is not able to completely empty the washing well, check the
condition of the tube and make sure that the space between the hinged guide and the rotor is
approximately 1.5 mm. Any necessary adjustments (in order to optimize pump aspiration in
relation to the newly substituted tube) can be made by turning the relative screw (Fig. 5) using the
Peristaltic Pump Setting Gage as illustrated in figure 6 (See Service News N° 9 dated 21.05.2003)
Screw for regulating peristaltic

pump aspiration
Fig. 5

Peristaltic Pump Setting Gage

Cod. 01-01201-00
Fig. 6
6.4 REACTION PLATE
6.4.1 REACTION PLATE - MECHANICAL ADJUSTMENT
1. Turn on the Liasys system and launch the “Analyzer” program. Remove the reaction plate cover
(Fig. 1).
2. Launch the diagnostic program, select the "Optic" function and turn on the light by clicking on
the designated key.
3. Reset the filter wheel by clicking on the appropriate key.
4. Select key “Spare” to bring filter position # 9 to the 'reading' position.
5. Select the "Plate" function. Reset by clicking on the appropriate key. Make sure that the "Home
Sensor" Reaction Plate window lights up green.
6. Make sure that the light beam inside the cuvette in centered with respect to the sides of the
cuvette. If not, adjust the OS3 optic sensor regulating bar (Fig. 2).
7. To exit the diagnostic program press the "Diagnostic" key
8. To exit the analyzer program press “shutdown” key

Reaction Tray Cd. 05-00366-00 Analysis plate fastening screw
Fig. 1 Fig.2
Cross-bar
OS3 Opto coupler assy Cd. 9-10-0023-00
Reaction Plate cover Regulating bar
6.4.2 SUBSTITUTION OF THE REACTION PLATE
procedure
1. Remove the reaction plate cover (Fig. 1).

2. Unscrew the four cross-bas fastening screws and remove the cross-bar (Fig. 1).
3. Unscrew the four anchored screws of the reaction plate module (Fig. 3).
4. Remove the J1 connector and the ground wire from the Reaction Chamber Motor Interface.
5. Remove the J1, J14 connectors and the ground wire from the Reaction Plate Interface.
6. Remove the top cover of the washing station using the special wrench supplied by the
manufacturer.
7. Disconnect the seven tubes attached to the probes on the washing station.
8. Remove the reaction plate from the instrument and substitute it with the new one.
N.B.: After substituting the reaction plate, carry out the mechanical adjustment procedure
of the reaction plate as described in Section 6.4.1

Reaction tray interface

Motor plate interface board
Cd. 30-00193-01
Cd. 30-00341-00
Anchored screw

* Reaction Plate assembly is supplied without Washing Station and Photometer
6.4.3 SUBSTITUTION OF THE OS3 OPTIC SENSOR
procedure.

4. Remove the J1 connector and the ground wire from the Reaction Chamber Motor Interface(Fig.
3).
5. Remove the J1, J4 and J14 connectors and the ground wire from the Reaction Plate Interface
(Fig.3).
6. Open the cover panel of the peristaltic pump assembly and disconnect the seven tubes attached to
the probes on the washing station.
8. Remove the fastening screw from the analysis plate using the special tool supplied by the
manufacturer (Fig. 2).

9. Unscrew the two fastening screws from the bottom cover panel of the washing station and lift it.
(Fig. 4).
10. Unscrew the two fastening screws on the cuvette holder assembly and lift it. This will make it
possible to remove the analysis plate (Fig. 5).
11. Unscrew the two fastening screws on the optic sensor and substitute it with the new one.
Fastening screws on the bottom

cover panel of the washing station
Fig. 4 Washing Station probes assembly Cd. 9-10-0006-00
Cuvette Holder Solenoid

Cd. 9-10-0060-00
Fastening screw on the cuvette holder assembly
Fig. 5 Cuvette Holder
N.B.: After substituting the optic sensor, carry out the mechanical adjustment procedure of
the reaction plate as described in Section 6.4.1

6.4.4 SUBSTITUTION OF THE M3 MOTOR
procedure.

4. Remove the J1 and J4 connectors and the ground wire from the Reaction Chamber Motor
Interface (Fig. 3).
5. Remove the J1, J14 connectors and the ground wire from the Reaction Plate Interface (Fig. 3).
6. Open the cover panel of the peristaltic pump assembly and disconnect the seven tubes attached
to the probes on the washing station.
8. Unscrew the four fastening screws that hold the motor support (Fig. 7).
9. Remove the four fastening screws on the motor (Fig. 8).
Warning: Belt tension must be regulated by turning the M4 screw (shown in Fig. 7) clockwise
by 1.3 Kg/cm.
Motor support fastening screw
Belt tension screw
Fig. 7

Belt for reaction plate

Cd 9-35-0032-00
Fig. 8
M3 Stepper Motor assembly (motor with pulley)
Cd. 9-10-0016-01
N.B.: After substituting the motor, carry out the mechanical adjustment procedure
of the reaction plate as described in Section 6.4.1

6.5 WASHING STATION ASSEMBLY
6.5.1 SUBSTITUTION OF THE WASHING STATION ASSEMBLY

4. Remove the J1and J3 connectors and the ground wire from the Reaction Chamber Motor
Interface (Fig. 2).
5. Remove the J1, J3, J5, J6, J14 connectors and the ground wire from the Reaction Plate Interface
(Fig. 2).
6. Remove the top cover of the washing station using the special tool supplied by the manufacturer
(Fig. 5).
7. Disconnect the seven tubes attached to the probes on the washing station (Fig. 6).
9. Unscrew the four fastening screws on the washing station assembly (Fig. 3).
10. Remove the washing station assembly and replace it with the new one.
12. After substituting and remounting, carry out the mechanical adjustment and functional check
procedures as described in section 6.5.4
Reaction plate cover
Cross-bar
Fig. 1

Reaction tray interface Cd. 30-00193-01
Motor plate interface board Cd. 30-00341-00

* Reaction Plate assembly is supplied without Washing Station and Photometer
OS4 Opto coupler assy

Cd. 9-10-0023-00
Washing station fastening screw
Motor support
Mechanical Joint screws
Flywheel screw
Stepper Motor (washing probes)

Cd. 9-10-0017-00
Fig. 3 Washing Station probes assembly Cd. 9-10-0006-00

6.5.2 SUBSTITUTION OF THE WASHING STATION OPTIC SENSOR
procedure.

4. Place the reaction plate vertically inside the instrument.
5. Remove the J3 connector from the Reaction Plate Interface (Fig.2).
6. Unscrew the two fastening screws on the optic sensor and substitute it with the new one (Fig. 3).
6.5.3 SUBSTITUTION OF THE WASHING STATION MOTOR
procedure..

5. Remove the J3 connector from the Reaction Plate Interface (Fig.2).
6. Loosen the two flywheel and motor joint screws (Fig. 3).
7. Unscrew the four fastening screws on the motor support and remove it (Fig. 3).
8. Unscrew the four fastening screws on the motor, substitute it with the new one (Fig. 3).

6.5.3.1 WASHING STATION - MECHANICAL ADJUSTMENT AND

FUNCTIONAL CHECK
6.5.3.2 Washing Station - Horizontal Alignment
1. Turn on the "Liasys" system and launch the “Analyzer” program

2. Launch the diagnostic program and select the "Plate" function.
Reset by clicking on the appropriate key. Make sure the "Home Sensor" Reaction Plate window
lights up green.
3. Select the "Washing" function. Reset by clicking on the appropriate key. Make sure the "Home
Sensor" Washing Station window shows a green light.
4. Select several times "Home" and "Down" by clicking on the appropriate keys. Make sure the
washing station probes enter the reaction cuvettes freely and totally unhindered. Pay particular
attention to the drying pad placed on probe number five. If the drying pad does experience any
friction whatsoever, loosen the two fastening screws on the bottom cover panel of the washing
station and mechanically align the pad better (Fig. 5).
5. Upon completion of this procedure, tighten the fastening screws on the bottom cover of the
washing station and exit the diagnostic program by clicking on "Diagnostic".
6. Carry out the functional check of the washing station as described in section 6.5.4.3
Fig. 5 Fig. 6
Down Cover
Top cover fastening screw Tubing
Horizontal mechanical adjustment screws

6.5.4.2 WASHING STATION - VERTICAL ALIGNMENT

1. Turn on the "Liasys" system (first the instrument and then the computer).
3. Reset by clicking on the appropriate key. Make sure the "Home Sensor" Reaction Plate window
lights up green.
4. Select the "Wash" function. Reset by clicking on the appropriate key.
5. Make sure the "Home Sensor" Washing Station window shows a green light.
6. Select several times "Home" and "Down" by clicking on the appropriate keys. Make sure the
washing station probes enter the reaction cuvettes freely and totally unhindered. Pay particular
attention to the drying pad placed on probe number five.
7. The upper limit must be reached before the mechanical end of run. The lower limit must allow
the drying pad to touch the bottom of the cuvette.
8. The washing station does not normally need vertical mechanical adjustment. If, however, it does,
loosen the OS4 optic sensor fastening screws (Fig.3) and move it either up or down in order to
correctly position the washing station. Upon completion of this procedure, tighten the optic
sensor fastening screws and exit the diagnostic program by clicking on "Diagnostic".
9. Carry out the functional check of the washing station as described in section 6.5.4.3.
6.5.4.3 WASHING STATION - FUNCTIONAL CHECK
1. Turn on the "Liasys" system (first the instrument and then the computer).
2. Launch the "Liasys" program and, under System Monitor, carry out a wash cycle and reading
cycle of the cuvettes using water (WBL).
3. Check system efficiency by comparing the obtained WBL values against the range of acceptance
of WBL. Make sure there is no leakage of liquid inside the analyzer.

6.5.5 WASHING STATION - CUVETTE HOLDER ASSEMBLY
6.5.5.1 SUBSTITUTION OF THE CUVETTE HOLDER ASSEMBLY MAGNET
substitution procedure..

5. Remove the magnet’s two fastening screws (Fig. 7).
6. Remove connector J6 from the Reaction Plate Interface board (Fig. 2).
7. Remove the magnet and substitute it with the new one.
6.5.5.2 SUBSTITUTION OF THE CUVETTE HOLDER ASSEMBLY OPTO SENSOR

2. Remove connector J5 from the Reaction Plate Interface board (Fig. 2).
3. Remove the optic sensor's two fastening screws and substitute it with the new one (Fig. 7).
5. After substituting and remounting, carry out the mechanical adjustment procedures as described
in section 6.5.5.3

6.5.5.3 ADJUSTMENT AND FUNCTIONAL CHECK OF THE CUVETTE HOLDER

MAGNETIC TRIGGER
procedure.
1. Loosen the screw on the cuvette holder trigger (Fig. 7).

2. Rotate the reaction plate in such a manner as to position the trigger between two cuvettes (Fig.
8).
3. Manually push the pin toward the center of the solenoid - simulating magnetic attraction between
the two.
4. Position the trigger in such a manner that the top edge is at the same height as the top edge of the
cuvette. Tighten the cuvette holder trigger fastening screw (Fig. 8).
5. Turn on the "Liasys" system and launch the “Analyzer” program.
7. Reset by clicking on the appropriate key. Make sure the "Home Sensor" Reaction Plate window
lights up green.
8. Select the "Wash" function and reset by clicking on the appropriate key.
9. Make sure the "Home Sensor" Washing Station window lights up green.
10. Select "Home" and "Down" several times by clicking on the appropriate keys. Make sure the
"Home Sensor" Cuv. Holder window lights up green when the washing station is in the "Home"
position and lights up red when the washing station is in the "Down" position. If not, loosen the
fastening screw on the opto sensor flag and adjust to obtain the correct signal (Fig. 7).
11. To exit the diagnostic program press the "Diagnostic" key.
12. To exit the analyzer program press “Shutdown” key.

Cuvette holder trigger fastening screw
Fastening screw on the opto sensor flag
Support
Opto coupler assy Cd. 9-10-0023-00
Cuvette Holder Solenoid

Cd. 9-10-0060-00
Fig. 7 Cuvette Holder
Top edge of the cuvette holder trigger
Top edge of the cuvette
Fig. 8 Reaction cuvettes

6.6 TEMPERATURE CHECK AND ADJUSTMENT
6.6.1 REACTION CUVETTE TEMPERATURE ADJUSTMENT AND CHECK
1. Turn on the "Liasys" system and launch the “Analyzer” program. Wait approximately one hour
in order to allow the instrument to reach temperature balance (at room temperature 20°). The
warm-up time may vary from 30 minutes (at about 21° C room temperature) up to 100 minutes
(at about 18° C room temperature).
3. Carry out the cuvette # 16 in dispensation position.
4. Open the door and fill the cuvette # 1 with 50 microliters of distilled water.
5. Select “Configuration” function.
6. Digit the password (1234) in the appropriate window and press “OK”.
7. Insert the temperature probe (Model type Chemitec S 501 with probe ∅ max 0.9 mm) inside
cuvette #1, from the door, until it touches the bottom of the cuvette.
8. Make sure the temperature read, after 5 – 10 minutes, is 37.0 °C – 0.2 + 0.3. If not, move the
cursor within the "Plate" window to select the desired temperature and then click on "Save" to
memorize said temperature.
N.B.: counts increase correspond to temperature decrease.

counts decrease correspond to temperature increase
seven counts correspond to about one degree centigrade.
9. Remove the temperature probe from the cuvette and empty it.
10. Repeat the procedure from point 3 to 9 using in sequence cuvette # 16, 31, and 46.
11. To exit the diagnostic program press the "Diagnostic" key.
12. Carry out the "Glucose" test on seven samples using distilled water for sample and reagent.
13. Press, "Start" and once the seventh sample has been dispensed, press "Stop". Wait the sound
signal from the analyzer, that means the actual stop.
14. Insert the temperature probe all the way to the bottom of the cuvette in which the sampling
probe is dispensing. Make sure the temperature registered is 37.0 °C – 0.2 + 0.3.
15. If not, launch the “Diagnostic” program and select the "Configuration" function.
16. Digit the password (1234) in the appropriate window and press “OK”.

17. Move the cursor within the "Heater" window to select the desired temperature and then click
on "Save" to memorize said temperature.
18. Remove the temperature probe from the cuvette and exit from the “Diagnostic” program.
19. Carry out a washing cycle from “Start Work” mask in order to clean the cuvette.
20. To exit the “Analyzer” program press “Shutdown” key.
N.B.: counts increase correspond to temperature decrease.

counts decrease correspond to temperature increase
seven counts correspond to about one degree centigrade.
6.6.2 TEMPERATURE CHECK AND ADJUSTMENT USING KIT P/N 55-01204-00
IMPORTANT NOTICE
BEFORE APPLYING THE FOLLOWING PROCEDURE (PO 55-01204-00).
CHECK WHETHER THE INTEGRATED CHIP “TL O71xxx” IS INSTALLED ON THE

“CONTROL PANEL BD. – POS. U2 (see fig. 1)” AND REPLACE IT WITH THE
INTEGRATED CHIP “LMC 7101 A or B or TVL 2461 cp.”
Temperature check and adjustment
This document describes how to adjust and check the pre-heater and plate temperature of the
Instrument System.
1. Training:
• Service Engineers must be trained on the use, maintenance and main operations of Instrument
System as well as on others tools involved in this procedure.
2. Summary:
INTEGRATED CHIP “LMC 7101 A or B or TLV 2461 cp.” BEFORE APPLYING THE
FOLLOWING PROCEDURE.

• Turn on the system and wait in order to allow the instrument working temperature settlement.
The warm-up time may vary from 30 minutes (at about 21° C room temperature) up to 100
minutes (at about 18° C room temperature).
• Calibrate the temperature probe by using the A and B resistors
• Put 300 µL (approx.) of distilled water PRE-WARMED at 37.0 ÷ 37.3°C into the cuvettes # 45
and 46
• Measure the temperature into the cuvettes # 45 and 46 to get an indication on the plate
temperature
• Launch the temperatures test from the “Temperature” folder inside the “Diagnostic” program in
order to measure and/or slightly adjust them
3. Special tools:
KIT FOR TEMPERATURE ADJUSTMENT P/N 55-01204-00 composed by the following

items:
• Temperature probe
• Calibrator Resistor A (10 KΩ)
• Calibrator Resistor B (5,76 KΩ)

4. Procedure description:
With a thermostatic bath, heat 250 ÷ 500 mL of distilled water up to 37-37.5°C (see page #3 if a
thermostatic should be not not available).

The warm-up may vary from 30 minutes (at about 21° C room temperature) up to 100 minutes
• During warm-up time, remove the protection cover of the Control Panel Board (Front Side Light
Indicators) (See fig. 1).
• During warm-up time, launch the “Diagnostic” program and then select the “Temperature”
Folder in the “Diagnostic” Program
• During warm-up time, select Calibration Sensor and carry out the three steps by following the
indications showed on the screen.
• Replace the calibration resistor with the temperature probe.
Note 1: Now the temperature probe and the service probe function can be used as a thermometer.
Then route the temperature probe tip through the small door for cuvettes replacement. (See
fig. 2)
To avoid the temperature probe damage be sure that its wires do not interfere with the reaction plate
rotation.

By executing the reaction plate Reset, the cuvettes # 45 and 46 will be accessible through the
small door so allowing the following operations:
a. Pre-warm the micropipette tip by repeatedly aspirating and dispensing the liquid from/into the
250-500 ml container. Put 300 µL (approx.) of distilled water (pre-warmed at 37.0÷37.3°C into
the cuvettes # 45 and 46.
b. Wait 2-3 minutes then enter in the “Temperature” Folder and select “Temp. Test Run”.
c. Measure the temperature by dipping and gently shaking the temperature probe into the liquid in
the cuvettes.
d. Take note about the measured temperatures.
Note 2: The reaction plate temperature must be set at 37.0 ÷ 37.2 °C
e. If necessary, modify the value in the PLATE parameter field, (Configuration Folder in the
Diagnostic Program) by taking into account that:
7 COUNTS CORRESPOND TO ABOUT 1°C
If the difference should be higher than 2°C, wait a few more minutes and repeat steps a.-b.-c.-
d.-e.
f. Close the main cover and after having checked the correct setting of the reaction plate
temperature, select Temp. Test Run in the Temperature Folder inside the Diagnostic program.
g. Verify that the temperature probe or others tools are correctly positioned so to avoid their
interference with the moving parts of the instrument. During this procedure step in fact, the
Sampling arm, the washing Station and the Reaction Plate will become active.
h. By pressing START, the system will sample seven reaction cuvettes in sequence
i. At the end of the sampling cycles, the main cover will be unlocked.
j. Open the small door for cuvettes replacement. Measure and take note of the cuvettes
temperature starting from the seventh up to the first cuvette by dipping and gently shaking the
temperature probe into the liquid.
k. At the end of measurements, press WASH. The system is ready for a new cycle.

Note 3: The measure obtained from the seventh cuvette is the best indication of the pre-heater
temperature. The measure obtained on the first cuvette is the nearest to the reaction plate
temperature (previously adjusted to 37.0 ÷ 37.2 °C). The objective is to obtain the same
temperatures into all the seven cuvettes (37° - 37.2°C).
l. If necessary, modify the value in the ARM parameter field, (Configuration Folder in the
m. After any modification in the Parameter values repeat the steps from “f” through “k”.
n. At the end of the adjustment procedure, get out from the Diagnostic Program and from the
Instrument software, execute a WASH cycle then followed by the WBL.
Further information:
The reaction plate has been studied in order to maintain the dispensed liquids at the set
temperature and not to heat them up.
Consequently it is really important to adjust the pre-heater temperature as much as possible near to
the reaction plate temperature value (37.0 ÷ 37.2 °C).
If a thermostatic bath should be not available, then apply the following:


The warm-up may vary from 30 minutes (at about 21° C room temperature) up to 100 minutes
• During warm-up time remove the protection cover of the Control Panel Board (Front Side Light
Indicators). (See fig. 1)
• During warm-up time, launch the diagnostic program and select the Temperatures Folder in the
Diagnostic Program
• During warm-up time, select Calibration Sensor and carry out the three steps by following the
indications showed on the screen
• Replace the calibrator resistor with the temperature probe.
Note 1: Now the temperature probe and the service probe function can be used as a thermometer.
Route the temperature probe tip through the small door for cuvettes replacement.(See fig 2)
To avoid the temperature probe damage, to be sure its wires do not interfere with the reaction plate
rotation.
1. Verify that the temperature probe or others tools are correctly positioned so to avoid their
2. Select Temp. Test Run in the Temperature Folder inside the Diagnostic program.
3. By pressing START, the system will sample seven reaction cuvettes in sequence
4. At the end of the sampling cycles, the main cover will be unlocked.
5. Wait 10 minutes, open the main cover and the small door for cuvettes replacement. Measure
and take note of the cuvettes temperature starting from the third up to the first cuvette by dipping
and gently shaking the temperature probe into the liquid.
6. At the end of the measurements, press WASH. The system is ready for a new cycle.
Note 2: In this case, cuvettes 1 through 3 can give a good indication of the reaction plate
temperature.
7. If necessary, modify the value in the PLATE parameter field, (Configuration Folder in the
8. After any modification in the Parameter values repeat the steps from step “1” through “7”.

9. Close the main cover and after having checked the correct setting of the reaction plate
temperature, select Temp. Test Run in the Temperature Folder inside the Diagnostic program.
10. Verify that the temperature probe or others tools are correctly positioned so to avoid their
11. By pressing START, the system will sample seven reaction cuvettes in sequence
12. At the end of the sampling cycles, the main cover will be unlocked.
13. Open the small door for cuvettes replacement. Measure and take note of the cuvettes
temperature starting from the seventh up to the first cuvette by dipping and gently shaking the
temperature probe into the liquid.
14. At the end of measurements, press WASH. The system is ready for a new cycle.
Note 3: The measure obtained from the seventh cuvette is the best indication of the pre-heater
temperature. The measure obtained on the first cuvette is the nearest to the reaction plate
temperature (previously adjusted to 37.0 ÷ 37.2 °C). The objective is to obtain the same
temperatures into all the seven cuvettes (37° - 37.2°C).
15. If necessary, modify the value in the ARM parameter field, (Configuration Folder in the
16. After any modification in the Parameter values repeat the steps from “7” through “14”.
17. At the end of the adjustment procedure, get out from the Diagnostic Program and from the
Instrument software, execute a WASH cycle then followed by the WBL.

U2
Fig.1

Fig. 2

6.6.3 REFRIGERATION ASSEMBLY TEMPERATURE CHECK
Warning: Keep the cooling fan protection grid, placed at the bottom of the refrigerated rack,
unencumbered and free of dust.
1. Place a reagent container containing distilled water in a position of the refrigerated reagents rack.
2. Turn on the "Liasys" system (instrument and computer).
Wait approximately one hour in order to allow the instrument to reach temperature balance (at
room temperature 20° C).
3. Place the temperature probe in the reagent container and check that the temperature is
approximately 10°C lower than room temperature. If not, substitute the reagents racks holder
(Fig. 1).
4. Remove the reagent container containing distilled water and exit from the analyzer program
press “Shutdown” key.
Fig. 1
Refrigerated Reagent Rack assembly Cd. 9-15-0024-00

6.7 RACKS HOLDER ALIGNMENT
6.7.1 MECHANICAL ALIGNMENT OF THE SAMPLES RACKS HOLDER
Warning: A check of the mechanical alignment of the samples racks must be performed every time
a rack is removed (either for servicing or substitution).
1. Open the two front panels and remove the samples and STAT racks.
2. Remove the top cover of the samples (Fig.1).
3. Loosen the four fastening screws on the samples racks holder (Fig. 2).
4. Replace the top cover of the samples. Place empty samples cups in the first and last positions of
the racks and reinsert the racks.
5. Align the holes of the caps with those of the top cover of the samples.
6. Once again, remove the top cover of the samples, remove the racks and tighten the fastening
screws of the samples racks holder.
7. Reposition the racks and replace the top cover of the samples.
8. Close the two front panels.
Fig. 1 Fig. 2
top cover of the samples Samples racks holder

6.7.2 MECHANICAL ALIGNMENT OF THE REAGENTS RACKS HOLDER
Warning: A check of the mechanical alignment of the reagents racks must be performed every time
a rack is removed (either for servicing or substitution).
1. Open the front panel and remove the reagents racks.

2. Remove the top cover of the reagents (Fig. 1).
3. Loosen the four fastening screws on the reagents racks holder (Fig. 2).
4. Replace the top cover of the reagents. Place empty reagents cups in the first and last position of
the racks and reinsert the racks.
5. Align the holes of the reagents containers with those of the top cover of the reagents.
6. Once again, remove the top cover of the reagents, remove the racks and tighten the fastening
screws of the reagents racks holder.
7. Reposition the racks and replace the top cover of the reagents .
8. Close the front panel.
Fig. 1 Fig. 2
top cover of the reagents Reagents racks holder

Chapter 07 - MAINTENANCE
CHAPTER 07
- MAINTENANCE –
INDEX
7. MAINTENANCE……………………………………………………………………….2
7.1 PREVENTIVE MAINTENANCE………………………………………………………2
7.2 LIST OF PARTS SUBJECT TO WEAR AND USAGE………………………………..4
7.3 SAMPLING PROBE - CLEANING PROCEDURE……………………………………4
7.4 WASH STATION CANNULAS - CLEANING PROCEDURE..…………..…………..5
7.5 WASH SOLUTION BOTTLES - CLEANING……………………………..…………..5
7.5.1 WASH SOLUTION BOTTLES - CLEANING PROCEDURE…………………...……6
7.6 TIP WASHING ………...…………………………………………………………….....6
7.6.1 TIP - CLEANING PROCEDURE………………………………………………..……..7
7.6.2 PROCEDURE FOR REPLACING THE TIP………………………………………….11
7.7 HYDRAULIC CIRCUIT WASHING ………...…………………………..….………..12
7.7.1 HYDRAULIC CIRCUIT - CLEANING PROCEDURE…….…………………….…..12
7.8 CHANGING THE PERISTALTIC PUMP TUBES…………….…………………..…13
7.8.1 PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBES………..…...13
7.9 CHANGING THE PHOTOMETER LAMP………………………………………….. 15
7.9.1 PROCEDURE FOR REPLACING THE PHOTOMETER LAMP……………….….. 15
7.10 CHANGING THE REACTION CUVETTES………………………….…………….. 16
7.10.1 PROCEDURE FOR REPLACING THE REACTION CUVETTES………..…………16
7.11 CHANGING THE TUBES KIT………………………………………………………..17
7.11.1 PROCEDURE FOR REPLACING THE TUBES KIT…………………..………...…..17
7.12 TABLE C – LIST OF THOSE MAINTENANCE PROCEDURES THAT CAN BE
PERFORMED BY THE USER AND/OR BY THE MAINTENANCE TECHNICIAN
…………………………………………………………………………18
7.13 DECONTAMINATION PROCEDURE……………………………………………….20

7 MAINTENANCE
This chapter contains all those routine operations, which concern instrument maintenance. Said
procedures, listed and described below, should be carefully and scrupulously followed in order to
guarantee the manufacturer’s specifications and the perfect working order of the instrument over
time.
7.1 PREVENTIVE MAINTENANCE
MAINTENANCE SCHEDULE
Table A, illustrated below, lists all those procedures to be carried out by the user/operator and the
relative frequency schedule. Strict adherence to said schedule will guarantee the optimal operative
efficiency of the instrument.
TABLE A – MAINTENANCE SCHEDULE
FREQUENCY PROCEDURE NOTES
DAILY – Before launching Check the levels of all the wash See Table B
“Start Work” solutions (Rinse, Water and
Cleaning)
DAILY – Before launching Check the levels of Reagents, See Table B
“Start Work” Standards and Controls
DAILY – Before launching Check the levels of the Waste Bottles
“Start Work” and, if necessary, empty them
DAILY – Before launching Carry out a WBL cycle See the User’s Manual,
“Start Work” Chapter 03– Description
of Instrument Software
DAILY – After Shutdown Clean the Sampling Probe using See Procedure 7.3
either paper toweling or gauze

TABLE A – MAINTENANCE SCHEDULE
FREQUENCY PROCEDURE NOTES
EVERY TWO WEEKS Clean the Wash Station’s four See Procedure 7.4
cannulas
EVERY TWO WEEKS Clean the wash solution bottles See Procedure 7.5
(Rinse, Water and Cleaning)
ONCE A MONTH Clean the Tip See Procedure 7.6
ONCE A MONTH Clean the Hydraulic Circuit See Procedure 7.7
EVERY TWO MONTHS Change the Reaction Cuvettes See Procedure 7.10
EVERY SIX MONTHS Change the Peristaltic Pump Tubes See Procedure 7.8
EVERY SIX MONTHS Change the Tip See Procedure 7.6
ONCE A YEAR Change all the tubes (Tube Kit) See Procedure 7.11
EVERY 2000 HOURS Change the Photometer lamp See Procedure 7.9
• N. B.: the above-described maintenance schedule refers to that situation in which the
workload of the Analyzer is approximately 500 tests per day. The interval frequency
may vary according to the individual instrument’s daily workload.

7.2 LIST OF PARTS SUBJECT TO WEAR AND USAGE
Description Type Quantity Code

Reagents bottle 40 ml 30 pieces 9-65-0034-00
Reagents bottle 10 ml 25 “ AS-650121
Glass reagent bottle 7 ml 10 “ AS-900020
Reagents bottle 2 ml 25 “ AS-650122
Conical sample cups 2 ml 1000 “ AS-65-0001
Short sample cups 1 ml 1000 “ AS-65-0100
Washing solution bottle 2 lt 1 “ 9-35-0041-00
Kit tubing peristaltic pump 2 “ 9-65-0040-00
Reaction cuvettes 60 “ 9-65-0031-00
Drying Pad 1 “ 9-01-0038-00
Halogen Lamp 1 “ 9-35-0016-00
Tubes Kit – complete 1 “ 9-65-0027-01
Tygon tubing 1 mt. 1 “ 90-01253-00
Kit for E.V.Diluter connection 1 “ 99-00906-00
Probe 1 “ 9-05-0064-00
Probe new coating 1 “ 9-05-0064-01
Micro-Pump Assembly 1 “ 9-10-0028-01
Complete Probe Assembly 1 “ 9-10-0062-00
Comp Probe Assembly New C 1 “ 9-10-0062-01
Diluter head Teflon Fitting 10 “ C1-01-0042-01
Diluter fitting 10 “ C1-01-01222-00
E.V. Rinse fitting 10 “ C190-01254-00
Solenoid Valve 1 “ F-35-0019-00
Micro-Pump (up4) Assy 1 “ 05-00403-00
Manifold Assembly 1 “ 05-00405-00
Inlet/Outlet fitting for rinse… 1 “ 01-01224-00
Lamps kit 5 “ 9-65-0035-00
Na electrode 1 “ 35-00814-00
K electrode 1 “ 35-00815-00
Cl electrode 1 “ 35-00816-00
Reference electrode 1 “ 35-00817-00
Peristaltic Pump Tubing-head 1 “ 35-00830-00
ISE inlet tubing connection 6 mt 1 “ 35-00831-00
Cleaning Solution 2 x 250 ml 1 piece AS-RN-00-20
Rinse Solution 1 x 50 ml 1 piece AS-RN-00-21

7.3 SAMPLING PROBE – CLEANING PROCEDURE

1) Turn off the Analyzer
2) Use only lint-free paper toweling or gauze
3) Dampen the gauze or paper toweling with distilled water and clean the outside of the
sampling probe. Wipe the probe from the top downwards only! This is to avoid that
any bits of cloth, paper or lint fibers accidentally enter the probe itself.
4) The manufacturer suggests that once weekly the above-described cleaning procedure
be performed using, instead of only simple distilled water, a 5% sodium hypochlorite
solution to dampen the gauze and then be repeated using distilled water.
7.4 WASH STATION CANNULAS – CLEANING PROCEDURE

1) Turn off the Analyzer.
2) Place a sheet of paper under the wash station cannulas in order to keep any
extraneous material from accidentally falling into the cuvettes.
3) Use only lint-free paper toweling or gauze.
4) Dampen the gauze or paper toweling with distilled water and clean the outside of the
cannulas. Wipe the cannulas from the top downwards only! This is to avoid that any
bits of cloth, paper or lint fibers accidentally enter the cannulas themselves.
5) The manufacturer suggests that once weekly the above-described cleaning procedure
be performed using, instead of only simple distilled water, a 5% sodium hypochlorite
solution to dampen the gauze and then be repeated using distilled water.

7.5 WASH SOLUTION BOTTLES – CLEANING

During normal use and over time, mold and dust can build up inside the wash solution bottles.
For this reason, it is extremely important that they be periodically washed. Said cleaning must be
thorough and meticulous in order to insure that every trace of mold or residue be removed.
How often the bottles must be cleaned depends on their use and on the quality of the distilled water
used in that particular laboratory. However, the manufacturer recommends thorough washing at
least once every two weeks.
It is extremely important that the user not underestimate the risks associated with mold and dust
particles. They are to be regarded as a serious hazard as they can be the cause of instrument
malfunction.
The wash solution bottles are located on the right side of the analyzer.
7.5.1 WASH SOLUTION BOTTLES - CLEANING PROCEDURE
Pull the level sensor connectors out from the bottle caps.
2) Take the caps off the bottles and empty them.
3) Fill each bottle with a 5% sodium hypochlorite solution.
4) Clean the inside of each bottle using a bottlebrush in order to remove all traces of mold
and/or residue.
5) Leave the sodium hypochlorite solution stand in the bottles for at least ten minutes.
6) Empty the bottles, rinse them repeatedly and well with tap water, and then twice more
using distilled water.
7) Dry the bottles.
8) Fill the bottles with their proper solutions.
9) Replace the bottles in their respective housings.
10) Close the bottles and reconnect the level sensors.
11) Carry out two ‘Wash Cuvettes’ cycles and two ‘WBL’ cycles. Check system efficiency
by comparing the WBL values and the test results obtained against the laboratory’s
quality control values.

7.6 TIP WASHING

The Tip is used to dry the cuvettes after they have been washed. This drying process is carried out
via aspiration and therefore, over time, the Tip will necessarily absorb various contaminating
particles.
The manufacturer suggests that the Tip be replaced every six months. Said frequency may vary
depending on the workload of the individual laboratory and the operating conditions/environment of
the single instrument.
The Tip must be washed regularly in order to guarantee proper functioning and must be replaced
with a new Tip as necessary.
7.6.1 TIP - CLEANING PROCEDURE

1. Turn off the Analyzer.
2. Remove the top cover (lid) of the Wash Station using the specific tool included among the
instrument’s accessories.
Special tool
Lid
th
5 cannula
Blocking
screw
3. Remove the cannula that contains the Tip (the 5th cannula) and disconnect the aspiration
tube
4. Remove the spring
Aspiration tube
Spring

5. Once the cannula has been removed, immerge it in a 5% solution of sodium hypochlorite for
at least 15 minutes.
6. Attach a 10 ml syringe to the cannula as illustrated in Figure 6.
7. Aspirate and dispense the hypochlorite solution through the cannula (and the Tip) until the
latter is completely clean. This aspirating and dispensing forces the liquid through the Tip
fibers in both directions. Please see Figure 7.
Connect the
syringe to the
Sodium hypochlorite
cannula
solution
8. Once the Tip is clean, repeat the aspirating and dispensing cycle 10 more times using
distilled water, then disconnect the syringe.

9. Re-connect the aspiration tube to the

cannula
10. Re-position the cannula in its housing

being careful to not kink the
aspiration tube
11. Re-position the spring on the

cannula’s cylindrical axel

12. Close the lid, making sure that the

cylindrical tops of the cannulas fit
into the corresponding holes on the
lid
Locking Screw
13. Insert the blocking screw into its

setting and screw it down using the
Guidance Holes special tool included among the
instrument’s accessories
IMPORTANT: Before starting the instrument back up, manually move the wash station
upwards as far as it will go.

7.6.2 PROCEDURE FOR REPLACING THE TIP
1. Turn off the Analyzer.

2. Unscrew the screw that fastens the black top cover of the wash station, using the special tool
included among the instrument’s accessories.
3. Remove the wash station cover lid and take out the cannula containing the Tip. Be very
careful to not lose the spring.
4. Disconnect the aspiration tube, remove the used Tip, and replace it with a new one. Press
lightly to push the new Tip into place – be careful to not press too hard as this could deform
the Tip.
5. Insert the cannula containing the new Tip into a cuvette, pushing it down inside until it takes
on the shape of the inside of the cuvette.
6. Remove the cuvette, connect the aspiration tube to the cannula and reposition the cannula
back into its proper housing in the wash station. Be careful to not kink the aspiration tube
while doing so.
7. Reposition the spring in its proper housing. Remount the wash station coverlid making sure
that the cylindrical tops of the cannulas fit into the corresponding holes on the wash station
coverlid.
IMPORTANT: Before starting the instrument back up, manually move the wash station
upwards as far as it will go.
New Tip
Tip to be
replaced
Pull to remove the

used Tip
Push the new Tip into

the cuvette

7.7 HYDRAULIC CIRCUIT WASHING
During normal use and over time, mold and dust can build up inside the wash bottles and can have a
negative effect on the hydraulic circuit, compromising the correct functioning of the micro-pump
and valves. This, in turn, can lead to inefficiency in the sampling probe and cuvette washing system.
For this reason, it is extremely important that the hydraulic circuit be periodically washed. Said
cleaning must be thorough and meticulous in order to assure that every trace of mold or residue be
removed.
How often the hydraulic circuit must be washed depends on the operating conditions/environment
of the single instrument and the quality of the distilled water used in that particular laboratory. The
manufacturer recommends thorough washing at least once a month.
It is extremely important that the user not underestimate the risks associated with mold and dust
particles. They are to be regarded as a serious hazard as they can be the cause of instrument
malfunction.
The hydraulic circuit input cannulas are located inside the bottles on the right side of the Analyzer.
7.7.1 HYDRAULIC CIRCUIT - CLEANING PROCEDURE
1) Turn on the Analyzer.
2) Prepare a bottle containing 500 ml of a 5% sodium hypochlorite solution
3) Insert the three aspiration cannulas, located inside the liquids bottles, into the bottle
containing the sodium hypochlorite solution.
4) Have the instrument carry out a ‘WBL’ cycle and then a ‘Wash cuvettes’ cycle.
5) Wait for fifteen minutes. Clean and dry the three cannulas and then insert them into a bottle
containing distilled water.
6) Have the instrument carry out a ‘WBL’ cycle and then a ‘Wash cuvettes’ cycle.
7) Insert the three aspiration cannulas back into their respective bottles. Said bottles should
have, in the meantime, been cleaned and filled with a fresh supply of the required solution.
8) Have the instrument carry out a ‘WBL’ cycle and then a ‘Wash cuvettes’ cycle. Check
system efficiency by comparing the WBL values and the test results obtained against the
laboratory’s quality control values.

7.8 CHANGING THE PERISTALTIC PUMP TUBES
The manufacturer recommends that the Peristaltic Pump tubes be replaced every six months. Said
frequency may vary depending on the workload of the individual laboratory. The quality and
reliability of these tubes is fundamental to a correct emptying of the cuvettes.
7.8.1 PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBES

2) Remove the panel located behind the wash station (Fig. 1).
3) Unhook the hinged guide by lowering and rotating the guide's blocking bracket to the left
(Fig. 2).
4) Once the hinged guide has been removed, pull the tubes out of their relative nipples.
Peristaltic Pump Wash station pumps assembly
5) Insert the new tubes into their relative nipples
6) Position the tubes around the peristaltic pump rotor.
7) Close back the hinged guide.

8) Manually rotate the peristaltic pump rotor clockwise and check to make sure that the tubes are
correctly positioned inside the guide.
9) Turn on the Analyzer and wait until the instrument has reached its proper operating
temperature.
10) Have the instrument carry out a ‘Wash cuvettes’ cycle and then a ‘WBL’ cycle. Check system
efficiency by comparing the WBL values and the test results obtained against the laboratory’s
quality control values.
11) Make sure that there is no leakage and then close back the panel.
Fig.2
Pin Hinged Guide Tubes to be replaced Nipples

7.9 CHANGING THE PHOTOMETER LAMP
The manufacturer suggests that the lamp be replaced after approximately 2000 hours of use.
Figure 3 illustrates the photometer lamp, its support base and its power supply wires. There is a
small hole on the lamp base useful for its mechanical alignment. The lamp is mounted on the
photometer, which is located on the right-hand side of the reaction plate.
7.9.1 PROCEDURE FOR REPLACING THE PHOTOMETER LAMP

2) Remove the reaction plate cover.
1) Disconnect the power supply wires from the Lamp Regulator Board by loosening the clamp
screws on the M1 connector (Fig. 4).
4) Unscrew the lamp’s fastening screw and remove the lamp from its housing (Fig. 4)
5) Replace the old lamp with a new one making sure that the pin is correctly inserted in the
alignment hole (Fig. 4). Remount the assembly by repeating the above steps 4 through 1 in
inverse order.
6) Turn the Analyzer on and wait until the instrument has reached its proper operating
temperature.
7) Have the instrument carry out a ‘WBL’ cycle. Check system efficiency by comparing the
WBL values and the test results obtained against the laboratory’s quality control values.
Lamp Holder Lamp
Bracket
Screw N°1
Alignment
Reference Point Lamp Power Supply
Connector
Fig.3 Fig.4
WARNING! DO NOT TOUCH THE GLASS PART OF THE LAMP WITH YOUR FINGERS!
IF NECESSARY, USE A CLEAN CLOTH TO REMOVE DUST, OR ALCOHOL TO REMOVE MORE
STUBBORN DIRT.

7.10 CHANGING THE REACTION CUVETTES
Over time and through normal use the perfect transparency of the cuvettes diminishes. This less-
than-perfect transparency has a negative impact on the quality of the optical readings. The
manufacturer suggests that the cuvettes be replaced after two months of use. The cuvettes are
located inside the reaction plate.
7.10.1 PROCEDURE FOR REPLACING THE REACTION CUVETTES

1. Turn on the Analyzer and wait until the instrument has reached its proper operating
temperature.
2. Click on “Change”, located under the work program (Please see Chapter 03 – Description of
Instrument Software).
3. A pull-down menu will appear. Click on “Plate – First Half” and then on OK.
4. A dialog box will appear asking: “Do you want to change the cuvettes requested?” Click
on OK to confirm. Remove the reaction plate coverlid.
5. Remove the cuvettes numbered 1 through 30 by VERTICALLY lifting them out from their
housing and then replace them with new cuvettes. Make sure to reinsert the new cuvettes
VERTICALLY. Moreover, be especially careful to not touch the external surface of the
cuvettes dedicated to photometric reading.
6. Select “Plate – Second Half” from the pull-down menu and then click on OK.
7. A dialog box will appear asking: “Do you want to change the cuvettes requested?” Click
on OK to confirm.
8. Remove the cuvettes numbered 31 through 60 by VERTICALLY lifting them out from their
housing and then replace them with new cuvettes. Make sure to reinsert the new cuvettes
VERTICALLY. Moreover, be especially careful to not touch the external surface of the
cuvettes dedicated to photometric reading.
9. Have the instrument carry out a ‘WBL’ cycle. Check system efficiency by comparing the
WBL values and the test results obtained against the laboratory’s quality control values.

7.11 CHANGING THE TUBES KIT

Over time and through normal use, the tubes become worn.
The manufacturer suggests that the tubes be replaced at least once a year. These tubes are located
behind the panel situated behind the wash station.
7.11.1 PROCEDURE FOR REPLACING THE TUBES KIT

2) Remove the panel located behind the wash station.
3) Replace the tubes following the indications provided in the hydraulic diagram (SI
00457-00).
4) Turn on the Analyzer and wait until the instrument has reached its proper operating
temperature.
5) Have the instrument carry out a ‘Wash cuvettes’ cycle and then a ‘WBL’ cycle. Make sure
that there is no leakage.
6) Check system efficiency by comparing the WBL values and the test results obtained against
the laboratory’s quality control values.

7.12 TABLE C – LIST OF THOSE MAINTENANCE PROCEDURES THAT

CAN BE PERFORMED BY THE USER AND/OR BY THE
MAINTENANCE TECHNICIAN
PROCEDURE FREQUENCY (*) EFFECTUATED

BY
CLEANING THE SAMPLING PROBE DAILY OPERATOR
CLEANING THE FOUR WASH STATION EVERY TWO

OPERATOR
CANNULAS WEEKS
CLEANING THE WASH SOLUTION EVERY TWO

OPERATOR
BOTTLES WEEKS
EVERY TWO
REPLACING REACTION CUVETTES OPERATOR
MONTHS
CLEANING THE TIP ONCE A MONTH OPERATOR
REPLACING THE TIP AS NEEDED OPERATOR
AS NEEDED
ALIGNING AND ADJUSTMENT OF THE
(e.g.: after replacing
SAMPLING ARM OPERATOR
any mechanical
(Std&Ctr/Reagents/Samples/Dispensing)
part)
REPLACING THE PERISTALTIC PUMP EVERY SIX

OPERATOR
TUBES MONTHS
AFTER 2000
REPLACING THE PHOTOMETER BULB OPERATOR
HOURS OF USE
REPLACING THE SAMPLING PROBE AS NEEDED OPERATOR
MAINTENANCE
REPLACING THE TUBES (Tubes Kit) ONCE A YEAR
TECH

PROCEDURE FREQUENCY (*) EFFECTUATED BY
REPLACING THE PRE-HEATER AND THE AS NEEDED MAINTENANCE

SENSOR LEVEL TECH
MECHANICAL ALIGNMENT OF THE AS NEEDED MAINTENANCE

SAMPLING ARM TECH
REPLACING OR ADJUSTING THE OPTIC AS NEEDED MAINTENANCE

SENSORS TECH
REPLACING THE BELT AS NEEDED MAINTENANCE

TECH
REPLACING A MOTOR AS NEEDED MAINTENANCE

TECH
ALIGNING THE WASH STATION/REACTION AS NEEDED MAINTENANCE

PLATE TECH
ALIGNING THE CUVETTE BLOCK AS NEEDED MAINTENANCE

TECH
REPLACING THE PHOTOMETER AS NEEDED MAINTENANCE

TECH
ADJUSTING THE PHOTOMETER AS NEEDED MAINTENANCE

TECH
REPLACING THE ELECTRONIC AS NEEDED MAINTENANCE

BOARDS/CARDS AND THE MECHANICAL TECH
MODULES
N. B.: the above-indicated frequency intervals may vary according to the individual instrument’s
daily workload.

7.13 DECONTAMINATION PROCEDURE

Before replacing any instrument parts, repairing any defective items or performing any instrument
maintenance procedure(s), the operator or maintenance technician must carry out the below-
described decontamination procedure of the instrument part(s) involved in the operation(s).
This procedure can be performed on:

the entire Analyzer
those part(s) of the instrument subject to possible contamination
Material necessary
- an ESOFENOL solution diluted to 6% (60 cc in one liter of distilled water). ESOFENOL

is an antibacterial and antiviral substance.
- Rubber gloves
- Mask
- Lab coat
External surfaces and individual parts
Î Spray the solution all over the instrument, paying particular attention to wetting:
- the sampling arm
- the reaction plate (including the cuvettes)
- the racks
- the instrument chassis
Î Allow the solution to stand for approximately 30 minutes

Î Wipe the solution off the instrument and the various components using a sponge dampened
with distilled water
Carry out a decontamination of the internal hydraulic circuit.
Hydraulic circuit (Entire Instrument)
Î Fill a container with a 5% sodium hypochlorite solution.

Î Disconnect the three silicon aspiration tubes from the nipples on the caps of their respective
tanks.
Î Immerge all three tubes into the container filled with the 5% sodium hypochlorite solution and
have the instrument carry out two ‘Wash cuvettes’ cycles and then two ‘WBL’ cycles.
Î Remove the three tubes from the 5% sodium hypochlorite solution and immerge them in another
container containing the instrument Rinse Solution.
Î Have the instrument carry out one ‘Wash cuvettes’ cycle and then one ‘WBL’ cycle.
Î Reconnect the three aspiration tubes to the nipples on their respective tanks.

Chapter 08 – Host Communication
CHAPTER 08
– HOST COMMUNICATION –
INDEX
8.1 COMMUNICATION WITH THE HOST COMPUTER .......................................................2
8.1.1 COMMUNICATION PARAMETERS .......................................................................2
8.2 PROTOCOL SPECIFICATIONS...........................................................................................3
8.3 HEADER RECORD (H)........................................................................................................4
8.4 PATIENT RECORD (P)........................................................................................................6
8.5 TEST ORDER RECORD (O)................................................................................................8
8.6 RESULTS RECORD (R).....................................................................................................11
8.7 MESSAGE TERMINATOR RECORD (L) ........................................................................13
8.8 REQUEST INFORMATION RECORD (Q):......................................................................13

8.1 COMMUNICATION WITH THE HOST COMPUTER
The Liasys can be connected to a host computer for the purpose of facilitating results print-out and
patient management.
In order to enable communication between the Liasys and the host computer, select the Host Link
field under Options in the Parameters menu.
To activate communication between the Liasys and the host computer, select Host–Tx (please see
the software description in Chapter 03 of the User’s Manual)
8.1.1 COMMUNICATION PARAMETERS
Communication parameters can be modified in the Parameters.ini file into the Analyzer 200 folder
for every need.
The Liasys PC is linked to the HOST computer using RS-232C serial connector having as default
communication parameters the following specifications:
o Transmission method : Asynchronous, half duplex

o Baud Rate : 9600 Bit/sec.
o Data bits: : 8
o Parity : None
o Stop bit : 1
o Connector : 9 pin type D (male output from the Liasys computer)
1-CF(DCD)
(DSR)CC-6
2-BB(RD)
(RTS)CA-7
3-BA(TD)
(CTS)CB-8
4-CD(DTR)
(RI)CE-9
5-AB(GND)
Serial connector

LIASYS PC HOST PC HOST PC

DB 9 F DB 25 F DB 9 F
3 Txd Rxd 3 2
2 Rxd Txd 2 3
5 SG SG 7 5
Serial Cable
8.2 PROTOCOL SPECIFICATIONS
This part of Help (Protocol Specifications) contains information for the laboratory computer and
analyzer. This exchange of data follows specific ASTM protocols:
E 1381-95 Standard Specification for Low-Level Protocol to Transfer Messages

between Clinical Laboratory Instruments and Computer Systems;
E 1394-97 Standard Specification for Transferring Information between Clinical
Instruments and Computer Systems.
ASTM uses a number of different terms to indicate the way it groups data.
• Field: an individual piece of data often referred to as a data field or a data element.
• Record: a number of logically related data fields grouped together to form one part of a
complete message.
• Repeat field: a data field of the same type as the one immediately preceding it. A delimiter
separates one instance of a repeat field from the next.
• Component field: part of data field that might contain more than one piece of data.
The default communication configuration for the Analyzer is the following: "9600,N, 8,1".
ASTM uses record types that are common and familiar to all laboratory personnel. It uses the
following record types:

• Header Record (H): contains identifying information about the sending station,
conventions that the device uses for field recognition, and the date and time of send station
transmission.
• Patient Record (P): contains patient information and identification number.
• Test Order Record (O): contains information about the assay or requests themselves and
includes other data.
• Result Record (R): contains information about the outcome of individual tests for an
individual patient and follows a sample program record. The results contain the actual
measurements derived from the test and a comparison of the individual result to certain
ranges specified as norms for the laboratory.
• Message Terminator Record (L): although the ASTM protocol supports three additional
record types - a Request for Information Record, a Scientific Record and a Manufacturer's
Information Record - the Analyzer is not implementing these in the first release and will
ignore them.
• Request Information Record (Q): is used by either clinical instruments or computer

systems for a remote request for information from its reciprocal system.
The instrument does not send or accept comment records.
8.3 HEADER RECORD (H)

Field Field Title Down Up Max Description and Valid Values
Load Load Len
1 Record Type R A 1 This is a required field that contains an

ID “H” identifying it as a header record.

2 Delimiters I A 4 The Analyzer System uses only the four

default values shown here. Delimiters may
not be duplicated. The field delimiter
follows the escape character to separate
the delimiter specification from a
subsequent field in the header record.
Using default values, the first six
characters of the header record will appear
using the following characters:
H I\^&I
Field Delimiter I
Repeat Delimiter \
Component Delimiter ^
Escape Delimiter &
3 Message I N
Control ID
4 Access I N
Password
5 Sender Name I A 10 ‘SHAnalyzer’: This is the name of the
or ID device that is sending the data.
6 Sender Street I N
Address
7 Reserved Field I N
8 Sender Tel. I N
Number
9 Characteristics I N
of Sender
10 Receiver ID I N
11 Comments or I N
Special
Instructions
12 Processing I N
13 ASTM Version I N
No.
14 Date and Time I A 14 Date and Time of transmission: formatted
as YYYYMMDDHHMMSS. For
example: 3:35 PM on March 1, 1995
would be represented using the following
characters:
19950301153500.

Legend: R Required D Down Load

O Optional U Up Load
I Ignored N Never
A Always S Sometimes
Example Header Record Layouts (H)

Download
Host H I \ ^ I I I HOST I I I I I I I I I19950301153500<CR>
Upload
Analyzer System H | \ ^ & | | | SHAnalyzer | | | | | | | | | 19950301154000<CR>
8.4 PATIENT RECORD (P)

Fiel Field Title Down Up Max Description and Valid Values
d Load Load Len
1 Record Type ID R A 1 This is a required field that contains a “P”

identifying it as a patient record.
2 Sequence Number R A 3 This field starts with a “1” for the patient
and is incremented by 1 for each
additional patient within the transmission.
3 Practice Assigned R A 15 This field can be assigned by the
Patient ID instrument with no corresponding
download.
4 Laboratory I N
Assigned
Patient ID
5 Patient ID No. 3 I N
6 Patient Name O S 36 This field has two components:

• Last Name (up to 20 characters)
• First Name (up to 15 characters).
7 Mother's Maiden I N
Name
8 Birth Date O S 8 Formatted as YYYYMMDD: For
example, a birth date of December 1,
1980 would be represented as: 19801201
9 Patient Sex R A 1 The valid values are:
• M for Male
• F for Female
10 Patient I N The Analyzer System will ignore this field

Race/Ethnic at launch.
Origin

11 Patient Address O S 60 For Analyzer, this is a four- component

field:
• Address (25 characters)
• City (25 characters)
• State (2 characters e.g.: NY, IT)
• Zip (5 characters)
13 Patient Tel I N
Number
14 Attending I N
Physician ID
15 Special Field 1 I N
16 Special Field 2 I N
17 Patient Height I N
18 Patient Weight I N
19 Patient Known or I N
Suspected
Diagnosis
20 Patient Active I N
Medications
21 Patient's Diet I N
22 Practice Field No. I N
1
23 Practice Field No. I N
2
24 Admission Date O S 8 Admission date only.
and Discharge Formatted as YYYYMMDD.
Date (if desired)
25 Admission Status I N
26 Location O S 20
27 Nature of I N
Alternative
Diagnostic Code
and classifiers
28 Alternative I N
Diagnostic Code
and classification
29 Patient Religion I N
30 Marital Status I N

31 Isolation Status I N
32 Language I N
33 Hospital Service I N
34 Hospital I N
Institution
35 Dosage Category I N

I Ignored N Never
Example Patient Record (P)

Download
Host P | 1 | B108K | | | MW5910^Smith | | 19861002 | M | | Park Avenue^New

York^NY^10002 | | | | | | | | | | | | | 20020923 | | Hematology | | | | | | | | | |
Analyzer P | 1 | B108K | | | MW5910^Smith | | 19861002 | M | | Park Avenue^New

System York^NY^10002 | | | | | | | | | | | | | 20020923 | | Hematology | | | | | | | | |
8.5 TEST ORDER RECORD (O)

Fiel Field Title Down Up Max Description and Valid
d Load Load Length Values
1 Record Type ID R A 1 This is required field that

contains an “O” identifying it
as an order
2 Sequence Number R A 3 This field starts with “1” for

the first Test Order Record
and is incremented by 1 for
each additional Test Order
Record within the record.
This will be reset to “1”
whenever another patient
record is transmitted.

3 Specimen ID R A 15 Although the operator can

manually edit this field at
any time, the value of this
field is usually assigned by
the laboratory computer
before down loading. The
Analyzer uses and reports its
results based on the assigned
specimen ID.
4 Instrument I N
Specimen ID
5 Universal Test ID I N 9 This is a four-component

field:
I N • Universal Test ID
Code (not used)
I N • Universal Test ID
Name (not used)
R A
• Universal Test ID
Type (not used)
• Manufacturer's or
local code (6
characters):
This is the code defined in
the Analyzer.
6 Priority I N
7 Request Ordered I N
Date/Time
8 Specimen I N
Collected
Date/Time
9 Collection End I N
Time
10 Collection I N
Volume/Units
11 Collector ID I N
12 Action Code I N
13 Danger Code I N
14 Relevant Clinical I N
Info.

15 Date/Time I N
Specimen
Received
16 Specimen Type R A 1 This is a numeric field
indicating the type of
specimen:
The Imm. System uses the
following ASCII characters:
0= Serum
1= Urine
17 Ordering I N
Physician
18 Physician Tel. I N
Number
19 User Field No. 1 I N
20 User Field No. 2 I N
21 Lab Field No. 1 I N
22 Lab Field No. 2 I N
23 Date /Time Result I N

Reported Last or
Modified
24 Instrument I N
Charge
25 Instrument I N
Section ID
26 Record Type I A 1 The field indicates the
direction of the transmission:
O - Down Loading
F - Up Loading
28 Location or Ward I N
of Specimen
Collection
29 Nosocomial I N
Infection Flag
30 Specimen Service I N

31 Specimen I N
Institution
I Ignored N Never
Example Test Order Record Layouts (O)

Download
Host O | 1 | AR102 | | ^^^GLU | | | | | | | | | | | 0 | | | | | | | | | | O | | | | |
Analyzer System O | 1 | AR102 | | ^^^GLU | | | | | | | | | | | 0 | | | | | | | | | | F | | | | |
8.6 RESULTS RECORD (R)

Field Field Title Down Up Max
Load Load
Len
1 Record Type ID A 1 This is a required field that contains an
“R” identifying it as a Results Record.
2 Sequence A 3 This field starts with “1” for the first

Number result and is incremented by 1 for each
additional result within the record.
This will be reset to “1” when the results
from another test order record are
transmitted to the laboratory computer.
3 Universal Test ID I N This is a four-component field:
• Universal Test ID Code (not
I N used)
• Universal Test ID Name (not
I N 9 used)
• Universal Test ID Type (not
A
R used)
• Local Manufacturer's or local code
(6 characters) this is the code
defined in the Analyzer.
4 Data or A 10 ‘Data’ is a 10-character, floating point

Measurement field that includes the decimal point. The
value number of precision point digits will vary
according to the test and is configurable
on the Analyzer.

5 Units of Measure A 6 This is a field for up to 6 characters that

the operator defines for analytic
measurement.
6 Reference A 21 This field has two components; one
Ranges giving the lower limit and the other the
upper limit of the range. The format for
this field is N^N.
7 Result Abnormal A 2 This field indicates the normal status of
Flags the result. The following codes are valid
values:
L - Below Low normal
H - Above High normal
LL - Below Panic normal
HH - Above Panic normal
< - Below absolute low (under linearity)
> - Above absolute high (over linearity)
N - Normal
A - Abnormal
E – Edited
8 Nature of N
Abnormality
Testing
9 Result Status A 1 The Imm. System currently implements
only two valid values:
F - final results;
V - operator verified/approved result.
10 Date of Change N
in Instrument
Normative
Values or Units
11 Operator ID N
12 Date/Time Test N
Started
13 Date/Time Test A 14 Date and Time of test completion:
Completed formatted as YYYYMMDDHHMMSS.
14 Instrument ID N


I Ignored N Never
Example Result Record Layouts (R)

Upload
Analyzer System R | 1 | ^^^GLU | 70.97 | UL | 70^105 | N | | F | | | | 20020923114302 |
8.7 MESSAGE TERMINATOR RECORD (L)

Down Up Max
Field Field Title Description and Valid Values
Load Load Len
1 Record Type R A 1 This is a required field that contains an
ID “L” identifying it as an Message
Terminator Record.
2 Sequence R A 1 For a message terminator, this message
Number should always be “1”.
3 Termination R A 1 This indicates the cause of termination.
Code The following codes are valid values for
the Analyzer :
Null or N-normal termination

I Ignored N Never
Example Message Terminator Record Layout (L)

Host LI1IN
Analyzer System LI1IN
8.8 REQUEST INFORMATION RECORD (Q):

Field Field Title Down Up Max Description and Valid Values
Load Load Len
1 Record Type ID A 1 This is a required field that contains a

“Q” identifying it as a request.
2 Sequence Number A 1 It is always “1”.
3 Starting Range ID A 31 This field can either be:

Number
"ALL" - to mean all demographics
and tests being ordered should be sent

to the instrument at this time,

or can have two components:
• Computer system patient ID
No. (up to 15 characters);
• Computer system specimen
ID No. (up to 15 characters).
4 Ending Range ID N
Number
5 Universal Test ID N
6 Nature of Request N
Time Limits
7 Beginning Request N
Results Date and
Time
8 Ending Request N
Results Date and
Time
9 Requesting N
Physician Name
10 Requesting N
Physician
Telephone
Number
11 User Field No. 1 N
12 User Field No. 2 N
13 Request A 1 It is always "O" (requesting test
Information Status orders and demographics only).
Codes
Example Request Information Record Layouts (Q)

Download To
Analyzer System H | \^& | | | SHAnalyzer | | | | | | | | | 20020927100402

Q | 1 | ALL | | | | | | | | | | O
L|1|N

Chapter 09 - ERROR SIGNALING AND TROUBLESHOOTING
CHAPTER 09
- ERROR SIGNALING AND TROUBLESHOOTING -
INDEX
9.1 ERROR SIGNALING…………………………………………………………………………..……..2
9.1.1 SYSTEM ERRORS ..........................................................................................................2
9.1.2 RESULT FLAGS..............................................................................................................3
9.1.3 DESCRIPTION OF RESULT FLAGS..............................................................................4
9.2 TROUBLESHOOTING GUIDE .......................................................................................7
Liasys Service Manual Rev.07 01 June 2009-06-12 Page 1

9.1 ERROR SIGNALLING
This chapter is dedicated to a description of the error signalling which may occur during the
programming or carrying out of the various operations. Error signals can be divided into the
following two groups:
➢ System Errors
➢ Result Flags
9.1.1 SYSTEM ERRORS
Whenever a system error is detected, it is signalled via the activation of the below-listed warning
lights/buttons:
➢ Warning Light: a triangle-shape located in the upper, right-hand portion of the System Monitor
screen. It lights up yellow when activated.
If this yellow triangle does light up, the operator need simply click on it to access the relative visual
text warning message indicating the cause of the signalled anomaly (said window will open in that
area dedicated to viewing data).
Following is a list of possible visual text “Warning!” messages:
• Liquid Alarm I (Rinse Solution)

• Liquid Alarm II (Distilled Water)
• Liquid Alarm III (Cleaning Solution)
• Temperature Out-of-Range
• Host Serial Port cannot be opened
➢ Fatal Error: an “X”-shape located in the upper, right-hand portion of the System Monitor mask.
It lights up red when activated.
If this red “X” lights up, the operator need simply click on it to access the relative visual text
message explaining the cause of the Fatal Error (said window will open in that area of the mask
dedicated to viewing data).
Following is a list of possible Fatal Error visual text messages:
• Error on Internal Arm

• Error on External Arm
• Filter Error
• ADC Error – Sample Channel
• ADC Error – Reference Channel
• Reaction Tray Position Error
• Plate Error (Home sensor not found)
• Arm Vertical error
• Cuvette lock Error

• Wash System Error

• Diluter Error
• Interlock Open while Instrument is running
• Macro send Error
• Overflow Rx Buffer Micro
• Time out Error (Generic error)
• Invalid command error
• Invalid parameter error
• Check sum error
9.1.2 RESULT FLAGS
Result Flags are categorized under the following group headings, Each group is identified by a
symbol, as listed here-below:
X PHYSICAL ERRORS
R CONCENTRATION ERRORS
C CALIBRATION ERRORS
A OPTIC DENSITY ERRORS
E RESULTS EDITED MANUALLY
I ISE MODULE ERRORS
? PROGRAMMING ERRORS
Every Result Flag, signalling an error, is accompanied by a symbol representing the group it is part
of. The operator need only click on the small red square ( ) next to the Result Flag symbol to access
the visual text message explaining the cause(s) of the signalled error.
In the central column of the following tables, the user will find those symbols which signal the type
of error encounter, as used in the print-out of the final report. These symbols can be modified by the
operator, in the Parameters section, under print options.
WARNING! The use of the Error Symbols in the print-out of the results (inclusion and/or
exclusion) IS UNDER THE DIRECT AND SOLE RESPONSIBILITY OF THE USER

9.1.3 DESCRIPTION OF RESULT FLAGS
¾ X Physical Errors
Temperature Error: T Reaction temperature (Reaction Plate) is out-of-range.
No Sample S Either no sample or sample serum quantity below

minimum or above maximum level for the declared
container.
No Reagent: R Either no reagent or reagent level below minimum or

above maximum level for the declared container.
No Rack: * No Rack present during sampling.
¾ R Concentration Errors
Very Low and Very High: L-H Flags determined by test results out-of-range as setup in
the Methods.
Low Alert and High Alert: A Flags determined by test results out-of-range as setup in
the Methods.
Low and High Linearity Limit: G Flags determined by test results out-of- range as setup
in the Method.
Calculation Error: C Concentration calculation error due to foreseeable

causes (Asymptote).
¾ C Calibration Errors
RBL missing: * No Reagent Blank Level.
Calibration missing: * No Standard or no Calibration curve.
STD Replicate insufficient: * Insufficient number of valid Standard Replicates.
STD Replicate outside CV%: * Coefficient of Variation Percentage in the Standard

Replicates over the set value
Invalid Calibration: * Calibration curve not valid – either because it is not

monotonic or because the Fit is above the set value.

¾ A Optic Density Errors
Inversion: I Reaction direction not in line with that set-up.
End Point Limit: P Values over the limits setup in the Methods
Parameters
Depletion Limit: D Values over the limits setup in the Methods Parameters.
First Limit: * Values over the limits setup in the Methods

Parameters
FIT: F Values over the limits setup in the Methods Parameters.
RBL out-of-range: * Reagent Blank Levels outside the range Setup.
Sample outside Standard: # Sample absorbance outside the calibration curve.
¾ E Results Edited Manually
Results Edited: E This symbol automatically appears whenever the

operator has manually modified the obtained results.
This operation annuls all the symbols indicating errors
which, in this case, will not be viewed.
¾ I ISE Module Errors
Air in the ISE Module: * Air present in the ISE Module hydraulic circuit.
Calibrate A drift: * Calibrate A drift in the ISE Module.
Noise in the ISE Module: * Background noise present – values not reliable.
Out-of-Range in ISE Module: * Linearity values out-of-range.
¾ ? Programming Errors
All those software errors which are deemed unforeseeable are indicated using this symbol.

¾ Result Asterisks
In the event that the following situations and/or error conditions occur, the system will notify the
user via a visualization of two asterisks (**) in the Results field:
PHYSICAL ERRORS
1 – very little or no sample
2 – very little or no reagent
3 – no rack
CONCENTRATION ERRORS
4 – calculation error
5 – signalling depletion limit
PROGRAMMING ERROR
6 – all cases
In the following situations and/or error conditions, the results field will contain a “0”:
1 – if the result is less than zero

2 – if the result is equal to zero
OPTIC DENSITY ERRORS

3 – Flag signalling inversion

9.2 TROUBLESHOOTING GUIDE
PROBLEM ➢ POSSIBLE CAUSE SOLUTION

Repeatability of results insufficient ➢ Sample Probe dirty
Clean the Sample Probe as described in Chapter 07
Maintenance
➢ Hydraulic leak and/or air bubble in the hydraulic circuit

(sampling)
Check Sample Probe fit
Check the fit of the hydraulic tubes and their connection:
if necessary, substitute the tubes and/or adjust their
connections/fittings
➢ Wash solution is contaminated. If the wash solution

contains contaminating particles (e.g.: mold, dust, lint), these
micro-particles can cause errors during the WBL running.
Change the wash solution
Clean the Wash Solution Bottle(s) and carry out the
Hydraulic Circuit wash procedure as described in
Chapter 07 – Maintenance
➢ Deterioration of the Reagent(s)

Substitute the bad reagent(s)
➢ Reaction cuvettes not dried correctly after being washed

Check the Tip used to dry the cuvettes after washing to
make sure it is in good working condition. If necessary,
clean the Tip or change it, following the procedure
described in Chapter 07 – Maintenance
➢ Light bulb not stable

Light bulb nearing the end of its 2000-hour life cycle
duration, or premature deterioration. In both cases,
change the light bulb following the substitution
procedure described in Chapter 07 – Maintenance.
Insufficient volume/quantity of the ➢ This type of problem can present itself either when the
various Rinse, Cleaning and
instrument is first turned on due to a lack of liquid in one or
Distilled Water solutions.
more bottles, or during test running whenever the involved
liquid has been finished
Refill the required bottle. If the instrument is running
tests, it will automatically Pause. Wait until sampling is
suspended, then refill the involved bottle with the
required liquid (Rinse/Cleaning/ Distilled Water). Press
Start to continue running the rest of the programmed
tests. This operation will not adversely affect the already
run tests.

Chapter 10 – ISE MODULE
CHAPTER 10
– ISE MODULE -
INDEX
10.1 ELECTROLYTE MEASUREMENT SYSTEM ................................................................... 2

10.1.1 PRODUCT DESCRIPTION........................................................................................ 2
10.2 ELECTROLYTE MEASUREMENT SYSTEM FEATURES AND BENEFITS ................ 3
10.3 TECHNICAL SPECIFICATION .......................................................................................... 4
10.4 SYSTEM OPERATION – ISE THEORY............................................................................. 5
10.5 MECHANICAL FEATURES ............................................................................................... 6
10.6 ELECTRODES...................................................................................................................... 7
10.7 FLUID MANAGEMENT ..................................................................................................... 8
10.7.1 REAGENT USED ........................................................................................................ 8
10.7.2 REAGENTS, CALIBRATION AND SAMPLE PROCESSING .................................. 9
10.8 ELECTRONICS .................................................................................................................. 11
10.8.1 GENERAL DESCRIPTION ...................................................................................... 11
10.9 MAINTENANCE ............................................................................................................... 11
10.10 TROUBLE SHOOTING GUIDE ....................................................................................... 12

10.1 ELECTROLYTE MEASUREMENT SYSTEM
10.1.1 PRODUCT DESCRIPTION
Electrolyte Measurement System includes a small, simple, and reliable ISE module and two
peristaltic pumps designed to be mounted within a existing chemistry analyzer. The module
measures the concentration of Sodium, Potassium and Chloride. The module contains an integral
sample entry port, positioned at the top. The compact design allows for small sample size and fast
operation. The modules requires only a 70 µl sample.
The module housing contains snap-in, snap-out ISE sensor which, through simple connectors,
connect directly to an electronic board in close proximity to the module. This eliminates the need
for cables and maximizes noise immunity. Samples and calibrators are positioned within the module
by two snap-in, snap-out peristaltic pump cassettes. The waste pump positions the sample in front
of the sensor for measurement. After sample measurement, a calibrant/wash solution is pumped in
front of the sensor for a single point calibration.
Provision for two-point sensor calibration and cleaning are made through use on the analyzer
sample tray which serve as reservoirs for the second calibrator and cleaning solution.
The module is completely self-contained. All sample and calibrant positioning within the module is
controlled by an integral microprocessor, which assure reliable electrode operation and maximum
lifetime.

10.2 ELECTROLYTE MEASUREMENT SYSTEM FEATURES AND

BENEFITS
Features Benefits
Integral Sample Entry port Minimal Sample Carry Over
Small Sample Size 70 µl
Electrodes Mounted Close to Electronics Minimal electronic noise improves precision
Sample Port Adjacent to ISE Sensor Minimal Sample Size and Carry Over
Rapid Operation (30 Sec Cycle Time) Rapid electrolyte results
Easy to access sensors Simple Maintenance
No membranes Maintenance performed by lab personnel
Easily accessed pumps Simple maintenance
Two bubbles detectors Reliable sample processing assured
Two point calibration High accuracy and precision
One point calibration with every sample High accuracy and precision
Maintenance free electrodes Low cost per test

(6 month or 10.000 sample warranty)
Disposable reference electrode, Convenaient maintenance

no addition of filling solution required

10.3 TECHNICAL SPECIFICATION
Sample: Whole Blood, Serum, Plasma or Urine

(Urine requires dilution 1:10)
Sample Size: 70 µl, (3 channels) serum; 160 µl diluted urine
Reproducibility: Maximum imprecision (within run) Typical Carry Over, % (Serum)

Sodium CV<1,5% (100 - 160 mmol/L) <0,5%
Potassium CV<2% (3,00 - 6,00 mmol/L) <1,5%
Chloride CV<2% (80,0 - 120,0 mmol/L) <1,0%
Analysis Time: Serum – 30 seconds, including one point calibration

Urine – 60 seconds, including one point calibration
Throughput: Serum – 120 sample/hour

Urine – 60 sample/hour
Reagents: Calibrant “A”

Calibrant ”B”
Cleaning Solution
Urine Diluent
Maximum Environnemental Temperature: 38°C

(Host Analyzer requires working temperature within: 18°C < T < 32°C)

10.4 SYSTEM OPERATION – ISE THEORY

Electrolyte Measurements System measure sodium, potassium and chloride in biological fluids,
using ion selective electrode technology. The flow-through sodium electrode selective PVC
membrane tubing, specially formulated to be sensitive to sodium ions. The potassium and chloride
electrodes employ similar designs with appropriate selective membrane materials. The potential of
each electrode is measured relative to a fixed, stable voltage established by the double-junction
silver/silver chloride reference electrode. An ion selective electrode develops a voltage that varies
with the concentration of the ion to which it responds. The relationship between the voltage
developed and the concentration of the sensed ion is logarithmic, as expressed by the Nerst
equation:
E = E°+RT log (ε C)/nF
Where:
E = The potential of the electrode in sample solution
E°= The potential developed under standard conditions
RT/nF = A temperature dependent “constant”, termed the slope(s)
Log = Base ten logarithm function
ε = Activity coefficient of the measured ion in the solution
C = Concentration of the measured ion in the solution
A comparative method of measurement is utilized. First, the ISE module measures the potential
developed when the sample is positioned in the electrodes. Next, Calibrant A is positioned in the
electrodes. The difference in the two potentials is related logarithmically to the concentration of the
measured ions in the sample divided by their respective concentrations in the Calibrant solution.
Since the difference in potentials and the concentration of the sodium, potassium or the other ions in
the Calibrant solution are known, the computer can calculate the concentration of the ions in the
sample solution, in accordance with the Nerst equation, rewritten as:
E-E° = S log (Cx/Cs) or Cx = Cs x 10^ [(E-E°)/S]

Where:
E = ISE potential developed in sample solution
E° = ISE potential developed in the Calibrant solution
S = Electrode slope calculated during calibration
Cx = Concentration of ion in the sample
Cs = Concentration of ion in the Calibrant solution
“S”, the slope, is determined during calibration using Calibrants A and B, which have known levels
of sodium, potassium, and chloride. When an automatic calibration is initiated, the slope is
calculated between the second Calibrant A reading and the Calibrant B reading.
Excessive drift or noise reading will be flagged and the appropriate error message sent to the host
analyser from the ISE Module.
10.5 MECHANICAL FEATURES
The electrode housing contains each of the ion-selective electrodes, as well as the reference
electrode.
Two bubble detectors are also included at both the top and bottom of the electrode chain. These are
used to properly position the sample for measurement. A sample port is positioned directly above
the chain of electrodes on the top of the module.
An electronic signal processing board is attached to the electrode housing. This board includes high
input impedance operational amplifiers to detect the ISE signals and additional digital processing
circuitry serving as an A/D converter and providing an ASCII signal output to the chemistry
analyser.
Each of the electrodes can be easily removed from the front of the housing.

10.6 ELECTRODES
The electrodes are maintenance-free and are warranted on a prorated basis for up to 10,000 samples
or 6 months, whichever occurs first. Cleaning Solution, aspirated from an operator designed sample
cup, is used at least once a day at the end of the day in order to minimize protein build-up in the
fluid lines. A two-point calibration of the ISE module is also done at least once a day at the
beginning of the first sample run. If the user is running more than 50 samples a day, both cleaning
and calibrant must be performed after 8 hours by the host analyzer.
The entire double-junction reference electrode is disposable. The reference electrode is filled with
sufficient KCL so that no filling solution must be added during the lifetime of the electrode.
Electrodes require calibrant sampling at 30 minutes intervals for reliable operation, but this is
completely controlled by the Electrolyte Measurement System without any need for control by the
host analyzer or the operator.
The electrodes require a 10 times sample dilution for measurement of urine.
The ISE module depends on the host analyzer to perform the dilution function.
It is not necessary to regulate the electrode housing temperature provided that its environmental
temperature does not exceed 38° C. However, the electrode module should not be subject to
changes greater than plus or minus 8° C without recalibrating.

10.7 FLUID MANAGEMENT
10.7.1 REAGENT USED

The sample is aspirated from a sample cup and dispensed into the sample port at the top of the ISE
module. The sample is then positioned in front of the sensor using the bubble detector and the
Waste Pump.
Four reagents are needed to operate the ISE module.
Calibrant “A”:
Used as wash solution and single-point calibrator. Calibrant A is pumped into the sample port by
the Calibrant A pump and then positioned in front of the sensors
Calibrant “B”:
Used as the second point in two-point calibration. Calibrant B is aspirated from a cup on the
analyzer at least once a day or every 8 hours, depending upon the laboratory schedule.
A volume of 500 µl is sufficient for one day’s requirements.
The Calibrant “B” must be placed on the system just before use to prevent a change in values from
evaporation.
Cleaning Solution:
Should be run once a day to prevent protein built up or at 8 hours intervals if the ISE Module
performs greater than 50 samples per day
Cleaning Solution may be aspirated from a sample cup; 500 µl is sufficient for one day’s
requirements.
Urine Diluent:
This is required for urine samples. Urine samples must be diluted manually by a factor of 10 to
perform urine measurement.

10.7.2 REAGENTS, CALIBRATION, AND SAMPLE PROCESSING

The sequence of use of Calibrant A and patient samples during processing is as follows:
1. Sample deposited into ISE Module sample port by host analyzer;
2. Sample positioned in front of electrodes of Electrolyte Measurement System by the waste
pump;
3. Sample equilibration and reading occurs during 7 second period;
4. Calibrant A pumped into electrode module;
5. Calibrant A equilibration and reading occurs during 7 second period;
6. Results transmitted to the host analyzer;
7. ISE module ready for next cycle.
Pumpings of a small amount of Calibrant A are performed when the Electrolyte measurement
System is in “Standby” or when it is not being used in the “Sample” mode. This significantly
improves the performance of the electrodes. The Electrolyte measurement System must always be
supplied with power so that “pumping” can occur. Pumping occurs automatically, without
prompting by the host analyzer, beginning 30 minutes after the last sample or calibration was
performed.
During sample processing, a volume of 200 µl of Calibrant A solution is used for one point
calibration, sample, wash, and cleaning. A volume of 120 µl is used for each sip.
The Electrolyte Measurement System should perform a two points calibration at the beginning of
the sample run. If the user is running more than 50 sample a day, both cleaning and calibration must
be performed after 8 hours by the host analyzer. 140 µl of Calibrant B solution are used during two
point calibration. During two points calibration, electrode calibration slopes are transmitted by the
module for QC purpose and may be used by the operator to diagnose module performance. The
slope is defined as:
Slope = (EB-EA) / log (CB / CA)
Where:
CA = Calibration A concentration in mmol/L

CB = Calibration B concentration in mmol/L
EA = ISE potential developed in Cal A solution in mV
EB = ISE potential developed in Cal B solution in mV

These slopes are checked by the module’s electronic processor and an error code will be transmitted
if they are outside the required range.
Typical slopes are approximately 55mV/decade for Na and K and 45mV/decade for Cl.
Acceptable limit slopes are:
Slope (mV/decade) Range (mmol/L)

Serum Urine
Na 50-63 Na 20-200 20-1000
K 50-63 K 0,2-20,0 1-50
Cl 40-59 Cl 25-200 20-500
Liasys Service Manual Rev. 07 01 June 2009 Page

10
10.8 ELECTRONICS
10.8.1 GENERAL DESCRIPTION
ISE module electronics include all pre-amplifiers and perform microprocessor control of the fluid
pumps, A/D conversion and RS-232C communications. The microprocessors apply proprietary
mathematical algorithms to electrolyte sensor output voltage, converting them to clinical units of
mmol/L.
Sensor Inputs:
• Na Electrode; • Reference Electrode;
• K Electrode; • Upper Bubble Detector;
• Cl Electrode; • Lower Bubble Detector.
10.9 MAINTENANCE
The Electrolyte Measurement System has been designed to require very little operator maintenance.
The only daily maintenance required is to run the Cleaning Solution after the last sample of the day.
REPLACEMENT/PART 3 6 9 12
MO MO MO MO
Pump Tube X
Na Electrode X
K Electrode X
Cl Electrode X
Reference Electrode X
Reagents Refill REAGENT as required by testing needs
Recommended Maintenance/Replacement Schedule (more than 100 samples per day)

REPLACEMENT/PART 3 6 9 12
MO MO MO MO
Pump Tube X
Na Electrode 10.000 samples
K Electrode 10.000 samples
Cl Electrode 10.000 samples
Reference Electrode 10.000 samples
Reagents Refill REAGENT as required by testing needs

11
10.10 TROUBLE SHOOTING GUIDE
SYMTOM PROBLEM CORRECTION

1. RS232 cable is disconnected
System does not respond Reconnect or replace cable
or damaged.
2. Module connector has been
Replace board
damaged.
3. Component failure on
Replace board.
board.
Low Slope:
Remove and replace sensors to
Na or K <45mV/decade, 1. Misalignment of sensors.
re-seat.
Cl<35 mV/decade or
Replace Cal B first and retest. If
2. Deterioration of calibrator
still low, replace Cal A and
solutions
retest.
3. Deterioration of sensing Replace problem sensor and test
electrode.
High Slope: 4. Air bubble on Reference Remove electrode, tap to
Na or K>63mV/decade, Electrode membrane. dislodge bubble, replace, and
Cl>60 mV/decade recalibrate.
5. Deterioration of reference Replace reference electrode and
electrode. retest.
6. Interaction between sensing Replace Cl electrode only and
electrodes retest.
7. Temperature of the module Check the temperature and if
or liquid higher than 37° C. necessary change location
Noise error Flag 1. Deterioration of sensing Replace problem sensor and test
Single electrode electrode
a) Check that motor or
solenoid valve near module
2. Electrical noise spike from
is not activated during the
environmental source
read portion of the module
cycle.
b) Component failure on
module board. Replace
board.
Noise Error Flag 1. Deterioration of reference Replace reference electrode and
Multiple electrodes electrode retest.
solenoid valve near module
2. Electrical noise spike from
is not activated during the
environmental source
read portion of the module
cycle.
module board. Replace
board.

12
Drift Error Flag 1. Deterioration of

sensing Replace problem sensor and
Single electrode electrode test.
Purge the Cal A and recalibrate
2. May occur when new sensor the module. If the sensor is new
or new bottle of Cal A is it may initially drift as it
installed on system. rehydrates over the course of 15
minutes.
Drift Error Flag 1. Deterioration of Reference Replace reference electrode and
electrode retest
Multiple electrodes
2. Electrical spike from solenoid valve near module
environmental source is not activated during the
read portion of the cycle.
module board. Replace the
board.
3. May occur when new sensor Purge the Cal A and recalibrate
or new bottle of Cal A is the module.
installed on system.
a) Host instrument must
1. Insufficient sample pipetted
Air in Sample deliver 70 µl.
into module sample port
Increase dispensed volume
b) Insufficient sample in
sample cup for all tests
programmed.
2. Sample not positioned a) Pumps not connected
properly. properly.
b) Pump tubing obstructed or
tubing length is excessive.
a) Sensors are not properly
1. Sample and Cal A are compressed. Check
Air in Sample and Cal A
segmented with air. compression plate, spring
and seal.
b) Ensure that all sensor and o-
rings are in place.
2. Fibrin or salt is plugging the c) Use Cleaning procedure
sensor flow path <CLEN> for module
d) Disassemble module and
clean or replace sensor with
plugged flow path.
3. Air Bubble Detector failure Replace air bubble detector
4. Waste Pump failure Replace the waste pump.

13
a) Sensors are not properly

1. Cal B and Cal A are compressed. Check
Air in Cal B and in Cal A
segmented with air. compression plate, spring
and seal.
b) Ensure that all sensor and o-
rings are in place.
2. Fibrin or salt is plugging the

a) Use Cleaning procedure
sensor flow path
<CLEN> for module
b) Disassemble module and
clean or replace sensor with
plugged flow path.
3. Waste Pump failure. Replace the waste pump.
4. Air Bubble Detector failure. Replace air bubble detector.
a) Host Instrument must
1. Insufficient Cal B pipped
Air in Cal B deliver 70 µl. Increase
into module sample port
dispensed volume
b) Insufficient sample in
sample cup for all tests
programmed.
2. Sample not positioned a) Pumps not connected
properly. properly.
b) Pump tubing obstructed or
tubing length is excessive
for spring and seal.
Air in Cal A (no “Air” errors Replace Cal A bottle with a
1. Cal A bottle is empty
reported for Sample or Cal B) new one and recalibrate.
2. Tubing is disconnected. Reconnect or replace tubing
3. Cal A pump is not working a) Check electrical
properly. connections.
b) Replace pump cassette.
c) Replace motor.
4. Tubing is plugged, split or Replace tubing.
crimpted.

14

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LIASYS

Via E. Barsanti 17/A

CHAPTER DESCRIPTION REV.

INDEX TABLE OF CONTENTS 07

04 ELECTRICAL SCHEMES AND DRAWINGS 07

06 SETTINGS AND ADJUSTMENTS 07

09 ERROR SIGNALING AND TROUBLESHOOTING 07

Liasys Service Manual Rev. 08 01 October 2009 Page 1

• Chapter 02 SYSTEM DESCRIPTION

• Chapter 04 ELECTRICAL SCHEMES AND DRAWINGS

• Chapter 05 DIAGNOSTIC PROGRAM

• Chapter 06 SETTINGS AND ADJUSTMENTS

• Chapter 08 HOST COMMUNICATION

• Chapter 09 ERROR SIGNALING AND TROUBLESHOOTING

• Chapter 10 ISE MODULE

Liasys Service Manual Rev. 07 01 June 2009 P age 1

1.1 THE AIM OF THE TECHNICAL MANUAL ……………………………………………..…..2

1.1.2 MANUAL OUTLINE……………………………..……………………..……………..………..2

1.2 SYSTEM INTRODUCTION ................................................................................................ 3

1.3 PRECAUTIONARY MEASURES ....................................................................................... 3

1.3.1 CHEMICAL RISKS ................................................................................................... 5

1.3.2 ELECTRICAL RISKS................................................................................................ 5

1.3.3 MECHANICAL RISKS.............................................................................................. 4

1.4 GUARANTEE ....................................................................................................................... 5

1.5 TECHNICAL OPERATING FEATURES………………………………………….…...... 6

1.5.1 COMPUTER & SOFTWARE FEATURES ............................................................. .9

1.5.3 DIMENSIOS, WEIGHT & ENVIRONMENT......................................................... 10

1.5.4 INSTALLATION REQUIREMENTS..................................................................... 10

Liasys Service Manual Rev. 07 01 June 2009 Page 1

1.1 THE AIM OF THE TECHNICAL MANUAL

1.1.2 MANUAL OUTLINE

. Chapter 2 DESCRIPTION OF THE SYSTEM

. Chapter 4 ELECTRONICS SCHEMES AND DESIGNS

. Chapter 5 DIAGNOSTIC PROGRAM

Liasys Service Manual Rev. 07 01 June 2009 Page 2

. Chapter 6 SETTINGS AND ADJUSTMENTS

. Chapter 8 COMUNICATION WITH HOST

. Chapter 9 ERROR SIGNALS AND GUIDE TO RESOLVE ANOMOLIES

. Chapter 10 MODULE ISE

Liasys Service Manual Rev. 07 01 June 2009 Page 3

1.2 SYSTEM INTRODUCTION

Via E. Barsanti 17/A

Liasys Service Manual Rev. 07 01 June 2009 Page 4

1.3 PRECAUTIONARY MEASURES

1.3.1 Chemical risks

1.3.2 Electrical risks

1.3.3 Mechanical risks

Liasys Service Manual Rev. 07 01 June 2009 Page 5

Besides guarantee does not cover damage caused by :

CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF GUARANTEE

Description Type Quantity Code

Reagents bottle 40 ml 30 pieces 9-65-0034-00

Liasys Service Manual Rev. 07 01 June 2009 Page 6

Probe new coating 1 " 9-05-0064-01

Liasys Service Manual Rev. 07 01 June 2009 Page 7

1.5 TECHNICAL OPERATING FEATURES

♦ Fully automatic, random access, continuous loading,

ASSAY TYPE ♦ End Point, Initial Rate, Kinetic, Bichromatic, Differential,

TEST ENTRY MODE ♦ Selective, Batch, Profiles, STAT

THROUGHPUT ♦ 200 test per hour without ISE module.