SERVICE MANUAL

Ver. 2.00
October 2006
All Rights Reserved
© 2005 Mitsubis hi Kagaku Iatron, Inc.
This manual is copyrighted, and all rights reserved. No part of this manual or the products it
describes may be reproduced by any means or in any fromw ithout prior consent inwriting from
Mitsubis hi Kagaku Iatron, Inc.

The PATHFAST system is for In Vitro Diagnostic Use.

PATHFAST is a trademark of Mitsubishi Kagaku Iatron, Inc.

PATHFAST SERVICE MANUAL Ｉ

Table of Contents
I．Table of Contents
II．Revision History

1. Introduction
1-１ Intended Use
1-２ Characteristics
1-３ Specifications
1-４ Marks of Conformity
2. Precaution Instructions for Maintenance Work
2 –1 Environmental Conditions
2 –2 Caution Labels
2 –3 Safety Instructions for Servicing
3. Unpacking Procedure
3 –1. Environmental Requirement for setting and operating PATHFAST
3 - 2. Upacking Procedure
3 - 3. Procedure to Remove Fixtures (Y-axis, Z-axis and PZ-axis)
4. Mechanical Parts: Description and Replacing Procedure
4 –1 Introduction
4 –2 Outside View of PATHFAST
4 –3 Internal Units
4 –4 Maintenance of Syringe Unit
4 –5 Maintenance of Covers
4 –6 Replacing Belts for P-Axis and Z-Axis
4 –7 Maintenance of Y-Axis
4 –8 Maintenance of P-Axis Unit
4 –9 Maintenance of M-Axis Unit
4 –10 Maintenance of Door Lock Unit
4 –11 Maintenance of PMT Shutter Unit
4 –12 Maintenance of Temperature Sensors
5. Electronic Parts: Description and Replacing Procedure
5 –1 Overview of Electrical System
5 –2 Power Supply
5 –3 Heater Units
5 –4 FAN

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PATHFAST SERVICE MANUAL Ｉ

5 –5 System Controller
6. Software Configuration
6 –1 Block Diagram of Hardware/Electrical Parts
6 –2 Software Configuration
6 –3 Structure of Applications
6 –4 Control Process and Files
6 –5 Files and Folders
6 –6 Software Upgrade
6 –7 Data Backup
6 –8 LCD Touch Panel Calibration
7. Maintenance CF and Teaching Operation
7 –1 Maintenance Menu
7 –2 Teaching
7 –3 BCR OPERATION
7 –4 LOG MONITOR
7 –5 HOST MONITOR
7 –6 Editing H/L Ratio for PMT
8. Troubleshooting
8 - 1 Assay Remarks (Sample, QC and CAL)
8 - 2 Error Codes for Failed Calibration
8 - 3 System Error Codes
8-4 Messages shown in popup screen (Caution, Alert, Indication, Error, etc.)
9. Spare Parts List
10. Periodic Maintenance
10 –1 Tools and Disposables Needed
10 –2 Cleaning
10 –3 Teaching Check
10 –4 Sensor Check
10 –5 Sample Recognition Sensor Check
10 –6 PMT Check
10 –7 Temperature Check
10 –8 Leakage Check
10 –9 Dispense Check
10 –10 Running Check with Reagents
10 –11 Recording Instrument Information
10 –12 PM Check List
11. Attached Drawings
11- 1. Hardware
11- 2. Electronics

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PATHFAST SERVICE MANUAL II

II. Revision History

II –1. Table of Revision History

This service manual is published by Mitsubishi Kagaku Iatron, Inc.

This manual is the latest revision and all the information contained is correct at
the time of its printing. Please note that this manual may be revised without
prior notice, because of continuous improvement of instrument and/or upgrade of
software,

The following table shows the history of revisions made to this service manual.

Rev. No. Date Revised Reason of Revision

1.00 October/1/2005 1st Revision

2.00 October/1/2006 Revision based on update of Maintenance CF
software

II –2 . Details of Revisions

The details of each revision is explained in the followings.

Revision No. : 1.00

-. Reason of revision : Newly created. First Edition.
-. Contents revised : New document.

Revision No. : 2.00

-. Reason of reision: Revision based on update of Maintenance CF software
-. Contents Revised: Refer to the table in next page for details.

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PATHFAST SERVICE MANUAL II

Rev. No. Chapter or Contents of major changes Related Pages

Section
2.00 Addition of sections for Chapter 6 (6-6, I-2
6-7, 6-8）
Table of Change of title of Chapter 7 and change
Contents of construction and section titles
Addition of a section for Chapter 10
(10-10)
Chapter 2 Additional information for ２ － １ ． 2-1,2-2
Environmental Conditions
Chapter 4 Revised references because of structure 4-5,4-28,4-30,4-31,
change in Chapter 7. 4-36
Added adjustment procedure for PZ 4-42 - 4-44
Lower Sensor.
Chapter 5 Added adjustment procedure for Start 5-46 - 5-47
and Stop Switches.
Added adjustment procedure after 5-56 - 5-58
replacing PMT Calibration Unit.
Chapter 6 Added procedures for Program Update 6-6 - 6-18
(6-6), Data Backup (6-7) and LCD Touch
panel Calibration (6-8).
Chapter 7 All revised. Added description of All pages
Maintenance CF functions. Changed
structure and titles. Added description
for BCR MONITOR, LOG MONITOR,
HOST MONITO and, Editing H/L Ratio
for PMT. Added procedure for default
BCR settings for Handheld Barcode
Reader.
Chapter 8 Added measures for error with Tip Waste 8-16 - 8-17
Box sensor.
Chapter 9 Put remarks for not eligible parts for 9-1
service in the field. 9-2
Added note regarding Maintenance Tool
Kit

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PATHFAST SERVICE MANUAL II

Chapter 10 Changed frequency of Air Filter 10-4

replacement.
Added pictures of adjusting tool for 10-7
Reagent Cartridge Rack.
Changed references for Teaching Check. 10-11
Corrected Specification for Temperature 10-26
Check (in PM Check List)
Replaced MAINENANCE MENU screens 10-12 - 10-13,
10-15 - 10-16
10-18 - 10-19
10-21
Added procedure to record instrument 10-25
information in periodic maintenance . 10-26 - 10-27
Modified PM Check List based on
changes above.

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PATHFAST SERVICE MANUAL Chapter 1

Chapter 1 Introduction

This manual provides the information needed to provide servicing and maintenance
on PATHFAST instrument.

In this chapter, the overview of PATHFAST is described. (The content is the same as
that in Chapter 2 of Operators Manual.)

1-１ Intended Use

PATHFAST is a small, easy-to-use, multi-analyte instrument that provides in

vitro quantitative determinations using whole blood, heparinized plasma,
serum, or other samples determined by the protocol of the test being run.
PATHFAST can be used in a variety of settings, including a hospital laboratory,
clinic, or near-patient testing to provide highly sensitive and accurate analysis.

Reagents used with PATHFAST have been developed and manufactured in

specially prepared cartridges by Mitsubishi Kagaku Iatron, Inc. The principle of
measurement employed for the reagent system is CLEIA (Chemiluminescence
Enzyme Immunoassay).

PATHFAST instrument utilizes Magtration® technology for B/F separation in

several reaction steps.

Note) Magtration® is trade mark of Precision System Science Co., Ltd.,

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PATHFAST SERVICE MANUAL Chapter 1

1-２ Characteristics

１．Compact Space saving, compact design

２．Full-automation Fully automated processing of pipetting samples,
reaction with reagents and detection
３．Samples Whole blood, plasma, serum.
4．Multi-item Processing in Parallel
Up to six different samples or up to six different
items in a batch can be assayed.
5．Short Assay Time Less than 17minutes (depending on reagent protocol)
6．Easy to Use Specially prepared prepackaged reagents and
disposables.
No water, wash solution nor waste bottles are
needed.
7．High Sensitivity/Accuracy/Reproducibility
Small amount of materials can be detected by using
photon counting method with high accuracy and good
reproducibility.
8．Wide Dynamic Range
Auto-controlled mechanical iris shutter adjusts the
proper light intensity to PMT (photo-multiplier
tube), giving wide measurable range.
9．Minimum Cross Contamination
Possibility of cross contamination among samples is
minimized because of mono-test type reagents and
disposables.
10．Bar-code Control Assay names, factory-set calibration data and
reagent expiration date are given by reagent
barcode. Sample ID and user IDs are also available
through hand held barcode reader.
11．Sample Recognition Sensor
Whole blood can be automatically differentiated
from the others by sample recognition sensor.

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PATHFAST SERVICE MANUAL Chapter 1

1-３ Specifications

Instrument Type Desk-top

Throughput Samples: Maximum 6 samples/batch
Process Time: Less than 17 minutes*/6 samples
* Depends on protocol of each assay item, refer to
package inserts.
Type of Samples Whole blood, plasma, serum.
Temperature Control Heat Block L: 37.5 deg. C
Heat Block S: 65 deg. C
Heat Block T: 35 deg. C
Handling Volume 20∼700ul
Dispensing Accuracy 50ul--------- CV less than or equal to 2%
Note: with distilled water, RT 20 – 25 deg. C.
Wavelength 300∼650nm (PMT Sensitivity Peak: 461nm)
Data Storage Patient Data: 1000
QC Data : 1800
CAL Data : 300
Oldest data is lost when data overflows.
Power Requirement 100 - 240 VAC, 50/60 Hz
Power Consumption 360 VA
Protection against electrical shock: class 1, type B
Size W375 x D570 x H510 mm
Weight About 33kg

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PATHFAST SERVICE MANUAL Chapter 1

1-４ Marks of Conformity

PATHFAST complies with the following standards. :

-. IEC 61010-1:2001 (2nd Edition) (“Safety requirement for electrical equipment

for measurement, control and laboratory use; Part 1: General requirement)
-. IEC 610100-2-010:2003 (2nd Edition) for use with IEC 61010-1:2001, (“Safety
requirement for electrical equipment for measurement, control and
laboratory use; Part 2-010: Particular requirements for laboratory
equipment for the heating of materials)
-. EN 61010-2-101:2002, (Part 2: Particular requirements for In Vitro diagnostic
(IVD) medical equipment)
-. EN 61326:1997+A1:1998+A2:2001 Class B, (Measurement, control and test
device – EMC requirement)
-. UL 61010A-1
-. CAN/CSA-C22.2 No. 1010.1-92

Acronym Test Symbol Test Information

CE According to EU Directive 89/336/EEC and
73/23/EEC

UL/ｃUL According to test standards of UL

(Underwriters Laboratory), US and UL Canada

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PATHFAST SERVICE MANUAL Chapter 2

Chapter 2 Precautions for Maintenance Work

In this chapter, precautions to be taken by the engineer when performing

maintenance on PATHFAST are described.
Maintenance shall be performed by those people who have been trained on
servicing PATHFAST.

２−１．Environmental Conditions
The followings are required as environmental conditions for setting and
operating PATHFAST. Check if those conditions are kept at the place where
PATHFAST is set and operated.
1) Power Supply
Provide adequate AC power to meet with PATHFAST power requirement of
AC100 ∼ 240V+/-10% 50/60Hz, 360VA. Be sure the instrument can be
grounded to the earth. Avoid sharing power source with other instruments.

2) Environmental Conditions
-. PATHFAST is equipped with temperature controlling units for assay
processing. Keep the environmental temperature and humidity within
the following ranges.
Temperature: 15 - 30 deg. C
Humidity : 40 - 80 % (relative, non-condensing)
Maximum relative humidity 80 percent for temperature
up to 31 ℃ decreasing linearly to 50 percent relative
humidity at 40℃.
-. Place the instrument on a plain and vibration-free surface to avoid the
filter cover to be deformed
-. Place PATHFAST on a place with enough capacity to support the
instrument and other equipments/disposables used for assays.
-. Avoid placing PATHFAST near by instruments with large power
consumption, or close to possible sources of AC voltage changes and/or
electro-magnetic wave emission.
-. Avoid placing PATHFAST where air from air-conditioners or other
instruments hits directly or where it is exposed to direct sunlight.
-. Air is taken into the instrument for controlling temperature inside. Do
not obstruct the air inlet at the bottom of the instrument by putting some

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PATHFAST SERVICE MANUAL Chapter 2

obstacles such as papers.

-. Do not obstruct the exhaust openings on the rear top of the instrument.
Also be careful not to drop something through the openings.
-. PATHFAST shall be indoor use only (pollution degree is II)
-. PATHFAST shall be placed altitude up to 2000 m.
-. Installation category is II.

２−２ Caution Labels

PATHFAST is supplied with caution labels inside where you should be aware of
the dangers in operation and in maintenance of the instrument.

Pay enough attention not to get hurt when working with those parts or
operating the instrument. The caution labels are attached nearby where you
need to work carefully. They are listed below and are based on the
international standards.

Caution Label Indicative Abstracts

Heat Block S becomes hot (65 degrees C).
Be careful enough not to get burnt.

High Temperature
Samples assayed by PATHFAST are whole blood,
plasma, serum, etc. and they shall be treated as
potentially infectious materials. Also QC and reagents
Bio-Hazard in the reagent cartridges may contain potentially
infectious materials. The caution labels are attached
where possibility of contamination by those materials
is suspected. Use protective equipments when
operating or working on the instrument.
Piercers to make holes in aluminum seals of reagent
cartridges are located inside syringe unit cover. They
have sharp edges and so pay enough attention not to
be hurt when setting reagent cartridge rack or
Laceration
cleaning piercers.

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PATHFAST SERVICE MANUAL Chapter 2

２−３．Safety Instructions for Servicing

PATHFAST is an instrument to analyze patient samples for specific assay

items. Patient samples, reagents or other materials used for the assays are to
be treated as potentially infectious.
When servicing on PATHFAST, you should take appropriate measures, such as
listed in the followings, to avoid infections.
-. Wear disposable plastic gloves.
-. Wear mask
-. Wear protective glasses
-. Wear labo-coat , cap
Note: If samples, reagents or waste liquids stick onto the skins, perform
washout and disinfection. And consult with doctors.

When checking instrument performance by using QC and reagents, keep the

following precautions.
-. Use reagents and disposables designated by Mitsubishi Kagaku Iatron
Inc.
-. Strictly follow the instructions for usage, storage, handling, etc. described
in package inserts.
-. Do not use expired reagent cartridges.
-. When handling reagent cartridge, hold it by pinching the cartridge edge
so that you may not touch the aluminum seal and detection well.
-. Be aware not to put saliva in detection well by blowing the well, for
example, which may cause erroneous result.
-. When you see some bubbles in wells of reagent cartridge or liquid on the
aluminum seal inside, tap it on the desk softly before using it for assay.
-. Pay attention when handling used reagent cartridges, PATHFAST tips,
waste tip box because they are potentially infectious
-. Ethanol used for cleaning PATHFAST system is flammable and needs to
be carefully treated.

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PATHFAST SERVICE MANUAL Chapter 3

Chapter 3 Environmental Requirement & Unpacking Procedure

In this chapter, environmental requirement for setting and unpacking procedure of
PATHFAST instrument is described.

3 – 1. Environmental Requirement for setting and operating PATHFAST

Check if the environmental requirement for setting and operating PATHFAST is
kept according to the description described in [2-1 Environmental Conditions] of
Chapter 2.

3 - 2. Upacking Procedure
Step 1 Remove PP bands and cut PP tapes to open the upper cover.

Accessory
添付品箱 Box

Step 1

Card
仕 切り板Board

Step2
メフＬ２００
Cushions

Side Part 外装箱

of Outer Box

Step 2 Take out accessory box and a cardboard.

Then remove the cushions and outer carton box.
Step 3 Cut PP tapes on the four edges of bottom part of
outer box and take the inner box out. 内装
Inner Box

Inner Box Label

Cushions
メフＬ２００

Step 3 外装箱（底）
Bottom Part of Outer

Fragile Care Mark

Label Label

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PATHFAST SERVICE MANUAL Chapter 3

Step 4 Remove tapes fixing protection sheet and take off the sheet.

Step 4

Protection Sheet

Step 5 Cut PP tapes to remove the upper cover of inner box.

Then pull the side part of inner box upward to remove it.

Side part
個 装 箱 （of
ス リInner
ーブ） Box
Polyethylene
ポリシート
Sheet

Instrument
製品

Step 5
Step 6

Cushions
固定パ ット

Reinforcement
合板（ 個装 箱補 強） Board
Upper Cover
個装箱（of天 ）Inner Box

Bottom Part
個装 箱（底）

Step 6 Cut PP tapes on the four edges of lower inner box and remove the cushions.
Remove the polyethylene sheet to take out the instrument.

Step 7 Place the instrument on a desk, referring to “２−１．Environmental Conditions”.

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PATHFAST SERVICE MANUAL Chapter 3

3 - 3. Procedure to remove fixtures (Y-Axis, Z-Axis and PZ-Axis)

3 - 3 - 1. Y-Axis Fixture
Remove the fixture shown in Fig.1. No tool is needed.

Y-Axis Fixture

Fig. 1 Y-Axis Fixture

3 - 3 - 2. Z-Axis Fixture
Pull out Y-axis stage manually toward you and remove the Z-Axis Fixture. No
tool is needed.

Z-Axis Fixture

Fig. 2 Z-Axis Fixture

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PATHFAST SERVICE MANUAL Chapter 3

3 - 3 - 3. PZ-Axis Fixture
Remove four screws fixing the cover on the back of instrument, using hex
wrench (2.5mm).

Fig. 3 Removing cover on the back

Remove the thumb screw which fixes the PZ-Axis Fixture securing PMT unit.
No tool is needed.

PZ-Axis Fixture

Fig. 4 PZ-Axis Fixture

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PATHFAST SERVICE MANUAL Chapter 4

Chapter4 Mechanical Parts: Description & Replacing Procedure

In this chapter, description of mechanical parts and replacing procedure of those
parts are explained.

4 –1 Introduction

Before detaching/disassembling the parts from the instrument, shut down the
instrument power and unplug the power cable.

4 –2 Outside View of PATHFAST

Fig. 4-2-1 Outside View of PATHFAST

4 –2 –1 Tools To Be Used

1. Phillips Screw Driver Size #1

2. Phillips Screw Driver Size #2
3. Ball point Hex Wrench: 1.5mm x L77mm
4. Ball point Hex Wrench: 2 mm x L82mm
5. Ball point Hex Wrench: 2.5mm x L85mm
6. Ball point Hex Wrench: 3 mm x L100mm
7. Ball point Hex Wrench: 4 mm x L110mm
8. Hex Wrench(No Ball Point): 3 mm x L100mm
9. Pipe: Inner Diameter 4 mm x L100mm
10. Snap Ring Plier for axes
11. Scale : 150mm
12. Tension Gauge 0 –1000g or more

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PATHFAST SERVICE MANUAL Chapter 4

13. Wire Cutter

14. Lock Tite (Week)
15. Spacer for Air Filter Exchange
16. Gap Gauge (0.2mm)

4 –3 Internal Units

The instrument consists of the following major modules.

P-Axis Unit
Syringe Unit
M-Axis Unit
Z-Axis Unit
Y-Axis Unit
PMT Shutter Unit
Door Lock Unit
Electrical Device Unit
P-Axis Unit

Electrical
Device Syringe Unit
Unit

M-Axis Unit

Y-Axis Unit

Fig. 4-3-1 Internal Units-1

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PATHFAST SERVICE MANUAL Chapter 4

Door Lock
Unit Z-Axis Unit

PMT Shutter
Unit

Fig. 4-3-2 Internal Units-2

4 –4 Maintenance of Syringe Unit

Part Number Description

512-05-023-M00 Syringe Unit

Tools needed:
Phillips Screw Driver Size #2
Ball point Hex Wrench: 2.5mm
Ball point Hex Wrench: 3 mm

The Syringe Unit is a consumptive part. It needs to be replaced once three

years or less depending on the operating frequency.

Fig. 4-4-1 Syringe Unit

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PATHFAST SERVICE MANUAL Chapter 4

4 –4 –１ Procedure for Replacing Syringe Unit

1. Remove two screws fixing P-Axis Cover.

（Phillips Screw Driver Size #2）

P-Axis Cover
Fixing Screws

Fig. 4-4-2 P-Axis Cover

2. Pull out P-Axis cover toward you, after removing the two screws.

3. If the Cylinder Plate is lifting up plungers or pushing down the Tip Remover
Shaft, rotate the Pulley manually to readjust.

4. Then remove four screws fixing Syringe Unit.

（Ball point Hex Wrench: 3 mm）

Pulley

Syringe Plate
Plunger

Tip Remover Shaft

Syringe Unit
Fig. 4-4-3 Removing Syringe Unit Fixing Screws

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PATHFAST SERVICE MANUAL Chapter 4

5. Remove Syringe Unit by pulling it out toward yourself.

6. Follow the reverse procedure to reassemble the unit.

7. After assembling, check the positions of nozzles and tip holes referring to 7-2-3
Axis Teaching 2. @TP.Y.TipOn teaching procedure in Chapter 7

[Reference]
If the left-right position is displaced, follow the procedure below. The
movable range is very small.
1. Remove Stage Cover(ref. to 4-7-1)
2. Loosen two fixing screws of Tip Stand and move it in left-right direction.
(Use ball point Hex wrench, 2.5mm)
3. After adjustment, perform teaching for 2. @TP.Y.TipOn.

Tip Stand fixing screws

Fig. 4-4-4 Tip Stand

4. Finally, perform the adjustment of the P-Axis ORG sensor.

[PORG] sensor is located as shown in the picture below.

PORG
XEL

Fig. 4-4-5 PORG Sensor

5. Check the gaps A and B in the following drawing for more than 0.2mm
each. (Use gap gauge of 0.2mm).

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PATHFAST SERVICE MANUAL Chapter 4

6. If they are not in the specification, loosen the fixing screw of PORG sensor
and move it in up-down direction. Then move P-Axis to Org position to
measure the gaps.
7. Repeat step 6 to adjust.

4 –4 –2 Replacing Stopper Against Rack Lifting

1. Remove the screw fixing Stopper against Rack lifting.

（Ball point Hex Wrench: 2.5mm）

Fig. 4-4-6 Removing Stopper against Rack lifting

Stopper consists of the following parts.

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PATHFAST SERVICE MANUAL Chapter 4

Fig. 4-4-7 Stopper against Rack lifting Put the edge

in the hole
2. Put the edge of the spring in the hole when setting Stopper.

Fig. 4-4-8 Warning in setting Stopper

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 Maintenance of Covers

Part Number Description

512-02-070-M0141 Side Cover L
512-02-070-M0151 Side Cover R
512-02-070-M0020 Front Cover
512-02-070-M0131 Lower Front Cover
512-04-030-M0410 Air Filter
512-04-510-K5110 Fan(Electronics)
512-04-510-K5100 Fan(Assay Area)

Tools needed
Ball point Hex Wrench: 1.5mm
Ball point Hex Wrench: 2.5mm
Ball point Hex Wrench: 3 mm

Outside covers are configured as follows:

Front Cover

Side Cover L

Rear Cover
Operation
Panel
Side Cover R

Lower Front
Cover

Printer Cover

Fig. 4-5-1 Outside Cover Configuration

The Operation Panel does not need to be removed other than for maintaining
the Electric Device Unit. The procedure to remove Operation Panel is
described in “
Maintenance of Electrical Devices”
.

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 –1 Removing Outside Covers

1. Side Cover L, R
First, remove Side Covers. (Picture below shows Side Cover, R)
The procedures to remove the Side Covers is almost same for both sides.
(Ball point Hex Wrench: 2.5mm)
<Procedure>
1. Remove two screws fixing the side cover.
2. Two Hanging Hooks are located on the back of the side cover. Lift the cover
upward to remove.

Hanging Hooks

Fixing screws for

Side Cover R

Fig. 4-5-2 Removing Side Cover R

2. Lower Front Cover

Before removing Lower Front Cover, you need to remove the brightness
adjustment knob of LCD display.
(Ball point Hex Wrench: 1.5mm)

1. Open Printer Cover.

2. Loosen the fixing screw for the brightness adjustment knob.
(Not needed to remove the screw.)
3. Pull the knob out.

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PATHFAST SERVICE MANUAL Chapter 4

Brightness adjustment
knob of LCD display

Fig. 4-5-3 Removing brightness adjustment knob

4. Remove fixing screw as follows.

(Ball point Hex Wrench: 3 mm)

Fixing Screw

Fig. 4-5-4 Removing Lower Front Cover-1

5. Then loosen the two fixing screw inside. Do not remove them, just loosen
them.

Fixing Screws for Lower

Front Cover

Fig.4-5-5 Removing Lower Front Cover-2

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PATHFAST SERVICE MANUAL Chapter 4

6. Now you can pull out Lower Front Cover.

Lower Front
Cover

Fig. 4-5-6 Removing Lower Front Cover-3

2. Rear Cover

To remove Rear Cover, follow the procedure below.

(Ball point Hex Wrench: 2.5mm)
<Procedure>
1. Remove six fixing screws in the following order.

Fixing Screws for

Rear Cover

Fig.4-5-7 Right side view of Rear Cover

Fixing Screws for

Rear Cover

Fig. 4-5-8 Rear side view of Rear Cover

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PATHFAST SERVICE MANUAL Chapter 4

Fixing Screws for

Rear Cover

Fig. 4-5-9 Left side view of Rear Cover

Fixing Screw for

Rear Cover

Fig. 4-5-10 Inside instrument

2. After removing all the screws, remove Rear Cover by lifting upward with
pushing the lower right part outward.

Push this part

toward out side

Fig.4-5-11 Removing Rear Cover-1

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PATHFAST SERVICE MANUAL Chapter 4

Fig. 4-5-12 Removing Rear Cover-2

3. Re-assemble the cover in the reverse order.

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 –2 Removing Front Cover

Front Cover is a part of Rear Cover and normally it may not be removed without
removing Rear Cover from the instrument. When you attempt to remove Front
Cover, follow the procedure below.
<Procedure>
1. Remove the fixing screws for Front Cover.
(Ball point Hex Wrench: 2.5mm)

Fixing Screws
for Front Cover

Fig. 4-5-13 Fixing Screws for Front Cover

Be careful not to drop the small parts (shown below) when removing the
screws.

Washer, supporting
point (SUS)

Resin Washer

Hinge Bushing

Fixing Screw

Fig. 4-5-14 Fixing Screw parts

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PATHFAST SERVICE MANUAL Chapter 4

2. Pull out Front Cover by detaching the arm of the Front Cover by bending
inward slightly.
Take care not to drop the Hinge Bushing which could be left on the wall.

Hinge Bushing

Fig. 4-5-15 Hinge Bushing

3. Re-assemble Front Cover in the reverse order, paying special attention to

the following.

- Put Hinge Bushing on the supporting point.

- Locate the hole of the arm of Front Cover on to Hinge Bushing.
- Put “Washer, supporting point (SUS)”and Resin Washer on the screw.
- Put small amount of weak Lock-Tight on the screw teeth.

Screw

Washer, supporting
point (SUS)

Resin Washer

Fig. 4-5-16 Fixing Screw

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 –3 Replacing Air Filter

<Procedure>
1. Put spacers under the front rubber feet.

Fixing Screw for Filter

Cover

Spacers

Fig. 4-5-17 Spacers

2. Remove Fixing Screw for Filter Cover manually.

Fixing Screw for Filter

Cover

Fig. 4-5-18 Replacing Air Filter-1

3. Pull out Filter Cover with Air Filter.

Fig. 4-5-19 Replacing Air Filter-2

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PATHFAST SERVICE MANUAL Chapter 4

4. Remove Air Filter from Filter Cover and replace with a new filter.

Fig. 4-5-20 Replacing Air Filter-3

5. Paying close attention to the location of hooks on the back and locating pins
on both sides, reassemble the Filter Cover in the reverse order.

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 –4 Removing Fan (Electronics) : FAN-2

<Procedure>
1. Disconnect the FAN-2 Connector for FAN-2.

FAN-2
Connector

Fig. 4-5-21 FAN-2 Connector

2. Remove four fixing screws for FAN-2.

(Ball point Hex Wrench: 2.5mm)
3. Pull out the cable with connector through the grommet and remove FAN-2.

FAN-2
Connector

Grommet

Fig. 4-5-22 FAN-2

4. Re-assemble FAN-2 in the reverse order.

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PATHFAST SERVICE MANUAL Chapter 4

4 –5 –5 Removing Fan (Assay Area) : FAN-1

<Procedure>
1. Disconnect the FAN-1 Connector. and remove the two fixing screws for the fan
bracket.
(Ball point Hex Wrench: 3mm)

Fixing screws for the fan

bracket

FAN-1
Connector
Fig. 4-5-23 FAN-1

2. Remove the two fixing screws for Fan (Assay Area).

(Ball point Hex Wrench: 3mm)

Fig. 4-5-24 FAN-1

3. Re-assemble FAN-1 in the reverse order.

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4 –6 Replacing Timing Belts for P-Axis and Z-Axis

Part Number Description

512-05-020-K0020 Z-Axis Timing Belt
512-05-021-K0020 P-Axis Timing Belt

Tools Needed
Ball point Hex Wrench: 2.5mm
Ball point Hex Wrench: 4mm
Scale, 150mm
Tension Gauge: 0～1000g

4 –6 –1 Replacing P-Axis Timing Belt

<Procedure>

1. Loosen the four fixing screws for P-Axis Motor.

2. Slide P-Axis Motor toward P-Axis Pulley.
3. Remove the P-Axis Timing Belt.
P-Axis Timing Belt

Fixing screws for

P-Axis Motor

P-Axis Motor

Fig. 4-6-1 Replacing P-Axis Timing Belt

4. Install the new belt and re-assemble in the reverse order.

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5. Tighten the four fixing screws for the P-Axis Motor. Using the tension gauge
and scale, checking that the belt tension is 2mm/500g.

F ig. 4-6-2 Measuring Tension of P-Axis Motor Timing Belt

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4 –6 –2 Replacing Z-Axis Timing Belt

<Procedure>

1. Loosen the four fixing screws for Z-Axis Motor.

2. Slide P-Axis Motor toward Z-Axis Pulley.
3. Remove the Z-Axis Timing Belt.
(Note) Place some cushion under Z-Unit before removing the belt.
When removing the belt, hold Z-Axis so that it descends to the cushion
softly.

Z-Axis Timing Belt

Fixing screws for
Z-Axis Motor

Z-Axis Motor

Fig. 4-6-3 Replacing Z-Axis Timing Belt

4. Install the new belt in the reverse order.

5. Tighten the four fixing screws for the P-Axis Motor. Using the tension gauge
and scale, checking that the belt tension is 3mm/500g.

Fig. 4-6-4 Measuring Tension of Z-Axis Motor Timing Belt

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4 –7 Maintenance of Stage (Y-Axis)

Part Number Description

512-05-031-M00 Heater Unit

Tools Needed
Phillips Screw Driver Size #1
(Ball point Hex Wrench: 2.5mm)

Stage (Y-Axis) is configured as follows and described as Stage or Y-Axis Unit.

Y-Axis Cover

Heater Unit

Tip Stand

Fig. 4-7-1 Stage (Y-Axis Unit) Configuration

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4 –7 –1 Removing Y-Axis Cover and Cover for Tip Waste Box

<Procedure>
1. Remove the fixing screws for Y-Axis Cover located two each on the front and
the rear of Y-Axis.
(Phillips Screw Driver Size #1)

Fixing screws for Y-Axis

Cover (Front)

Fig. 4-7-2 Fixing screws for Y-Axis Cover (Front)

Fixing screws for Y-Axis

Cover (Rear)

Fig. 4-7-3 Fixing screws for Y-Axis Cover (Rear)

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2. Lift Y-Axis Cover at the front.

3. Then lift Y-Axis cover at the rear until the surface touches to the bottom of
Magnet Unit.

Fig. 4-7-4 Removing Y-Axis Cover -1

4. Now you can pull out Y-Axis cover toward you.

Fig. 4-7-5 Removing Y-Axis Cover -2

5. Next, remove two fixing screws securing Cover for Tip Waste Box.
(Ball point Hex Wrench: 2.5mm)

6. Lift and remove Cover for Tip Waste Box.

Fixing screws securing

Cover for Tip Waste Box

Fig. 4-7-6 Removing Cover for Tip Waste Box

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PATHFAST SERVICE MANUAL Chapter 4

Fig.4-7-7 After Cover for Tip Waste Box removed

7. Re-assemble the covers in the reverse order.

4 –7 –2 Replacing Heater Unit

<Procedure>
1. Disconnect the connectors for Thermocouple(2), Heater(3) and Temperature
Fuse(2), in total 7 connectors.

Fig. 4-7-8 Heater Unit Connectors

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2. Remove four fixing screws securing Heater Unit.

（Ball point Hex Wrench: 2.5mm）

Fixing screws
for Heater Unit
(Front)

Fig.4-7-9 Fixing Screws for Heater Unit (Front)

Fixing screw for

Heater Unit (Rear)

Fig. 4-7-10 Fixing Screw for Heater Unit (Rear)

Fixing screw for

Heater Unit (Rear)

Fig. 4-7-11 Fixing Screw for Heater Unit (Rear)

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3. Lift Heater Unit to remove.

Fig. 4-7-12 Removing Heater Unit

Fig. 4-7-13 Heater Unit

4. Re-assemble it in the reverse order and perform teaching for the following
positions according to the procedure in Chapter 7, 7 –2 –3 Teaching
procedure for each position.
. -. 1. Teaching of @TP.Y.BlackWell
-. 3. Teaching of @TP.Y.PMT and @TP.PX.PMT(1).Center
-. 4. Teaching of @TP.Z.Proc.BlackWell

After the teaching finished, set Offset values for temperature controllers
following the procedure below.
<Procedure>
1. Confirm that the room temperature is in the range of 20 to 25 deg.
Centigrade.
2. Put 200ul of distilled water in detection wells of cartridges in 1 thru 6 lanes.
3. Put 600ul of distilled water in sample pretreatment wells of cartridges in 1
thru 6 lanes.
4. Put 200ul of distilled water in temperature check hole of Magnet Block.
5. Close Front Cover and leave the instrument for about one hour with
powered on.

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6. Measure the temperature in each wells and hole for three times using
Temperature Sensor HFT-40. Calculate each mean value.
Offset Value = Mean Value –Set Value
Ex)
Offset value = 37.0 (Mean Value) –37.5 (Set Value)
= - 0.5
7. Press MODE Key on the temperature controller for longer time and select
SET1.
8. Press MODE Key on the temperature controller for longer time and select
_PUS.
9.Us e▼▲ keyst oi nputo f
fsetval
ues .

4 –7 –3 Removing Waste Tip Sensor

Follow the procedure below to remove and /or replace Waste Tip Sensor.

<Procedure>
1. Remove Tip T Connector and Tip Connector.

Tip T Connector

Fig. 4-7-14 Tip T Connector

Tip Connector

Fig. 4-7-15 Tip Connector

2. Remove two fixing screws for the sensor. (Ball point Hex Wrench: 2.5mm)

3. Remove the sensor cable through the hole for the cable.

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PATHFAST SERVICE MANUAL Chapter 4

Indicator Lamp

Fixing Screws for

the sensor

Hole for
routing
cable

Fig. 4-7-16 Sensor

4. Re-assemble in the reverse order.

After assembling, perform teaching of Tip Waste Box, referring to the
procedure in 7 –2 –3 Teaching procedure for each position, 8．Teaching of
@TipTrashSense.Y.Center of Chapter 7.

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4 –7 –4 Replacing Sample Recognition Sensor Unit

<Procedure>
1. Remove two fixing screws for Sample Recognition Sensor Unit.
(Ball point Hex Wrench: 2.5mm)

Fixing screws for Sample

Recognition Sensor Unit

Fig. 4-7-17 Removing Sample Recognition Sensor Unit

2. Disconnect only the lower connector that connects the unit to PATHFAST
main circuitry.
Note) Do not disconnect the upper one.

Connector without
cable connected

Fig. 4-7-18 Sample Recognition Sensor Unit

3. Re-assemble in the reverse order.

4. Adjust the position of Sample Recognition Sensor Unit, referring to the

procedure in 7 –2 –3 Teaching procedure for each position, 2. Teaching of
@TP.Y.TipOn of Chapter 7. Then tighten the fixing screws.
5. Check AD values of sensors of Sample Recognition Sensor Unit, referring to
the procedure in section 10-5 of Chapter 10.

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4 –8 Maintenance of P-Axis Unit

Part Number Description

512-05-021-M00 P-Axis Unit

Tools Needed
Phillips Screw Driver Size #2
Ball point Hex Wrench: 2.5mm
Ball point Hex Wrench: 3 mm
Ball point Hex Wrench: 4 mm
Hex Wrench(No Ball Point): 3 mm
Pipe: Inner Diameter 4 mm x L100mm
Wire Cutter

Follow the procedure below to replace the P-Axis Unit.

<Procedure>
1. Remove the screws fixing P-Axis Harness Clumps.
(Ball point Hex Wrench: 3 mm)
2. Disconnect the connectors.

P-Axis Harness
Clump

Connector

Fig. 4-8-1 P-Axis Harness Clump -1

P-Axis Harness
Clumps

Connectors

Fig. 4-8-2 P-Axis Harness Clump -2

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3. Remove the fixing screws for Harness Holder to remove it.

(Ball point Hex Wrench: 2.5mm)

Fixing screws for

Harness Holder

Harness Holder

Fig. 4-8-3 Harness Holder

4. Remove the fixing screws for Block Support to remove it.

(Phillips Screw Driver Size #2)

Fixing screws for

Block Support

Block Support

Fig. 4-8-4 Block Support

5. Remove four fixing screws for P-Axis Unit.

(Hex Wrench(No Ball Point): 3 mm, Pipe: Inner Diameter 4 mm x L100mm)

Fixing screws for

P-Axis Unit

Fig. 4-8-5 Fixing screws for P-Axis Unit

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PATHFAST SERVICE MANUAL Chapter 4

6. Remove P-Axis Unit toward you.

Fig. 4-8-6 P-Axis Unit

7. Re-assemble the unit in the reverse order and follow the procedure in 4 –6 –1
for the belt tension adjustment, as needed.

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4 –9 Maintenance of M-Axis Unit

Part Number Description

512-05-022-M00 M-Axis Unit

Tools Needed
Ball point Hex Wrench: 2mm
Ball point Hex Wrench: 3 mm
Wire Cutter

Follow the procedure below to replace the M-Axis Unit.

<Procedure>
1. Disconnect connectors marked as M ORG, M and MH.
2. Remove the two fixing screws securing M-Axis Unit.
(Ball point Hex Wrench: 3 mm)

M ORG Connector

Fig. 4-9-1 M ORG Connector

M Connector
M H, TP-M Connector

Fixing screws for

M-Axis Unit
Fig. 4-9-2 Fixing Screws for M-Axis Unit (from bottom side)

Fig. 4-9-3 M Connector, M H and TP-M Connector

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3. Cut the tie wrap fixing the Compensatory Cable.

Tie Wrap

Fig. 4-9-4 Harness, M-Axis Unit

4. Remove the fixing screw for Thermocouple Connector.

(Ball point Hex Wrench: 2mm)

Fixing screw for

Thermocouple
Connector

Fig. 4-9-5 Fixing screw for Thermocouple Connector

Fig. 4-9-6 M-Axis Unit

5. Re-assemble the unit in the reverse order and follow the procedure in 7 –2 –3
Teaching Procedure for Each Position, 7. Teaching of @TP.M.BasePos of
Chapter 7 to adjust the teaching position.

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4 –10 Maintenance of Door Lock Unit

Part Number Description

512-05-072-M00 Door Lock
09401-070K022 Interlock Switch

Tools Needed
Ball point Hex Wrench: 3mm
Pipe: Inner Diameter 4 mm x L100mm
Wire Cutter

4 –10 –1 Replacing Door Lock Unit

Follow the procedure below to replace Door Lock Unit

<Procedure>.
1. Disconnect SOL SEN Connector and DOOR SOL Connector.

SOL SEN
Connector

DOOR SOL
Connector

Fig. 4-10-1 Connectors, Door Lock Unit

2. Remove the two fixing screws for Door Lock Unit.

Fixing screws for Door

Lock Unit

Fig.4-10-2 Fixing screws for Door Lock Unit

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PATHFAST SERVICE MANUAL Chapter 4

Fig. 4-10-3 Door Lock Unit

3. Reassemble in the reverse order. Do not tighten the screws firmly yet.
Re-tighten the screws after reassembling the unit and outer cover, adjusting
its position.

4. Put your hand inside through the window of Rear Cover and move the Door
Lock Pin to check if the pin moves smoothly through the hole (Refer to the
picture below). Move the Door Lock Unit so that the pin moves in and out
smoothly and tighten the fixing screws for Door Lock Unit.

Door Lock Pin

Fixing screws for Door

Lock Unit

Fig. 4-10-4 Fixing Door Lock Unit

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4 –10 –2 Replacing Interlock Switch

Follow the procedure below to replace the Interlock Switch.

<Procedure>
1. Disconnect the DOOR SW Connector.

DOOR SW
Connector

Fig. 4-10-4 DOOR SW Connector

2. Remove the two fixing screws for Interlock Switch.

(Ball point Hex Wrench: 2.5mm)

Adjusting
Screw

Fixing screws for

Interlock Switch

Fig. 4-10-5 Interlock Switch

3. Re-assemble in the reverse order.

4. Use the adjusting screw to set the switch in the proper position with the
following conditions.

-. It shall not be activated at position less than 5mm from the stage surface.
-. It shall not be deactivated when Front Cover is lifted upward under locked
condition.

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4 –11 Maintenance of PMT Shutter Unit

Part Number Description

512-07-510-k5680 Barcode Reader
512-05-11-M00 PMT Shutter Unit

Tools Needed
Ball point Hex Wrench: 2mm
Ball point Hex Wrench: 2.5mm
Wire Cutter
Barcode Reader is placed next to PMT Shutter Unit and the replacing procedure
of Barcode Reader is also described in this section.

4 –11 –1 Replacing Barcode Reader

<Procedure>
1. Remove the two fixing screws for Harness Cover to remove the cover.
（Ball point Hex Wrench: 2.5mm）

Fixing screws for

Harness Cover

Fig. 4-11-1 Fixing screws for Harness Cover

2. Disconnect the Barcode Reader Connector.

3. Remove the two fixing screws for Barcode Reader to pull out.
(Ball point Hex Wrench: 2.5mm)

Fixing screws for

Barcode Reader

Connector for Barcode Reader

Fig. 4-11-2 Fixing screws for Barcode Reader

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PATHFAST SERVICE MANUAL Chapter 4

Fig. 4-11-3 Barcode Reader

4. Re-assemble in the reverse order.

4 –11 –2 Replacing PMT Shutter Unit

Follow the procedure below to replace PMT Shutter Unit.

<Procedure>
1. Disconnect all the connectors from Connector Bracket, except the PZ ORG
and PZ L connectors.
(This work shall be done with the Harness Cover removed as described in
the previous section.)

PZ ORG connector
PZ L connector

Fig. 4-11-4 Connector Bracket

2. Remove two fixing screws for PMT Shutter Unit. Screws are attached from the
bottom through the bracket.
(Ball point Hex Wrench: 2mm)

3. Remove PMT Shutter Unit by widening the holding bracket slightly in the
direction of the arrow.
(See picture in the next page.)

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PATHFAST SERVICE MANUAL Chapter 4

Fixing screws for

PMT Shutter Unit

Fig. 4-11-5 Fixing screws for PMT Shutter Unit

4. Disconnect Power Connector and Signal Connector for PMT.

Fig. 4-11-6 PMT Connectors

Fig. 4-11-7 PMT Shutter Unit

5. Re-assemble in the reverse order.

The PMT Shutter Unit will click in the bracket smoothly.
6.After assembling, follow the procedure below to check or adjust the PZ Lower
Sensor position.
1. Turn the instrument powe r on with Maintenance CF installed.
2. Select AXIS OPERATION in the first screen.
3. Home Z-Axis by selecting ORG.
4. Home PX-Axis by selecting ORG.
5. Home Y-Axis by selecting ORG.

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6. Move PMT to PMT.Y.Center position.

7. Move PMT to PMT(5).PX.Center position.
8. Move Z-Axis down by 200 pulses.
9. Set the jig of 5.2mm part on the stage and check that LED of PZ Lower
Sensor is deactivated.

Jig for adjusting PZ Lower Sensor

Check that LED of lower

sensor is deactivated.

Put the jig of

5.2mm part

10. Set the jig of 5.8mm part on the stage and check that LED of PZ Lower
Sensor is activated.

Check that LED of lower

sensor is activated.

Put the jig of

5.8mm part

11. If LED Lower sensor is deactivated or activated in steps 9 or 10 above,

loosen the two fixing screws for Lower Sensor and adjust position by
moving it up or down.

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PATHFAST SERVICE MANUAL Chapter 4

Loosen two screws to adjust

sensor position.

Activate with 5.2mm, move upward

Deactivated with 5.8mm,move
downward

12. Repeat steps 9 and 10 for correct adjustment.

7. When adjustment completed, follow the procedure in 7 –2 –3 Teaching

Procedure of Each Position, 3. @TP.Y.PMT and @TP.PX.PMT(1).Center and 6.
@TP.PX.PMT.Home
8. When you replaced PMT Shutter Assembly or PMT Calibration Unit, check H
counts following the procedure described in 10 –6 PMT Check and edit H/L ratio
for PMT following the procedure described in 7 –6 Editing H/L Ratio.

4 –12 Maintenance of Temperature Sensors

Part Number Description

51205-510K-0040 Thermostat
510E0010 TEMP PCB Assy
Tools Needed
Ball point Hex Wrench: 2.5mm

4 –12 –1 Replacing Thermostat

Temperature sensor consists of Thermostat and TEMP PCB Assy.

Follow the procedure below to replace Thermostat

<Procedure>
1. Disconnect the connector for Thermostat.

Fig. 4-12-1 Connector for Thermostat

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PATHFAST SERVICE MANUAL Chapter 4

2. Remove the fixing screw for Thermostat and remove Thermostat.

(Ball point Hex Wrench: 2.5mm)

Fixing screw for

Thermostat

Fig. 4-12-2 Fixing screw for Thermostat

Fig.4-12-3 Thermostat

3. Re-assemble in the reverse order.

4 –12 –2 Replacing TEMP PCB Assy

Follow the procedure below to replace TEMP PCB Assy.

<Procedure>
1. Disconnect the connector for TEMP PCB Assy.
Connector for
TEMP PCB Assy

Fig. 4-12-4 Connector for TEMP PCB Assy

2. Remove the two fixing screws for TEMP PCB Assy and remove TEMP PCB

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PATHFAST SERVICE MANUAL Chapter 4

Assy
(Ball point Hex Wrench: 2.5mm )

TEMP PCB Assy

Fixing screws for

TEMP PCB Assy

Fig. 4-12-5 Fixing screws for TEMP PCB Assy

Fig. 4-12-6 TEMP PCB Assy

3. Re-assemble in the reverse order.

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PATHFAST SERVICE MANUAL Chapter 5

Chapter5 Electronics Parts: Description and Replacing Procedure

5 - 1 Overview of Electrical System

5 – 1 - 1 Block Diagrams
Refer to the attached drawings of following diagrams.
・ PATHFAST System Diagram (1) (51204-110E**)
・ PATHFAST Block Diagram (1) (51204-210E**)
・ PATHFAST Block Diagram (2) (51204-220E**)
・ PATHFAST Block Diagram (3) (51204-230E**)

5 – 1 – 2 Sensor Location

List of Sensors
Unit Sensor Name Label Type Maker
Ｙ-Axis Ｙ-Axis Original YORG PM-L24 SUNX
Ｙ-Axis +End Limit +YEL PM-L24 SUNX
Ｚ-Axis Ｚ-Axis Original ZORG PM-R24 SUNX
Ｚ-Axis Bottom ZB PM-U24 SUNX
Ｐ-Axis Ｐ-Axis Original PORG PM-R24 SUNX
Ｐ-Axis +End Limit +PEL PM-F24 SUNX
Ｍ-Axis Ｍ-Axis Original MORG PM-F24 SUNX
PMTX-Axis PMTX-Axis Original PXORG PM-F24 SUNX
PMTX-Axis +End +PXEL PM-R24 SUNX
Limit
PMTZ-Axis PMTZ-Axis Original PZORG PM-L24 SUNX
PMTZ-Axis Detect PZL PM-L24 SUNX
PMT PMT Shutter Open PSO PM-L24 SUNX
Shutter PMT Shutter Close PSC PM-L24 SUNX
Door Lock Door Lock Sensor DLS PM-L24 SUNX
Solenoid
Tip Waste Waste Tip Sensor TIP EX-21B SUNX
Inter-lock Inter-lock Door SW V-105-1A4 OMRON
Inter-lock Release Maintenance V-105-1A4 OMRON
SW

Refer to the following pages for sensor locations.

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PATHFAST SERVICE MANUAL Chapter 5

Ｙ-Axis Original Sensor (Front of Instrument)

YORG

Fig 5-1-1 Location of YORG Sensor

Ｙ-Axis +End Limit Sensor (Rear of Instrument)

+YEL

Fig 5-1-2 Location of +YEL Sensor

Ｚ-Axis Original Sensor (Rear of Instrument)

ZORG

Fig 5-1-3 Location of ZORG Sensor

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PATHFAST SERVICE MANUAL Chapter 5

Ｚ-Axis Bottom Sensor (Rear of Instrument)

ZB

Fig 5-1-4 Location of ZB Sensor

Ｐ-Axis Original and +End Limit Sensors (Front of Instrument)

+PEL

PORG

Fig 5-1-5 Location of PORG.+PEL Sensors

Ｍ-Axis Original Sensor (Front of Instrument)

MORG

Fig 5-1-6 Location of MORG Sensor

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PMTX-Axis Original Sensor (Rear of Instrument)

PXORG

Fig 5-1-7 Location of PXORG Sensor

PMTX-Axis +End Limit Sensor (Rear of Instrument)

+PXEL

Fig 5-1-8 Location of +PXEL Sensor

PMTZ-Axis Original and Detect Sensors (Rear of Instrument)

PZORG

PZL

Fig 5-1-9 Location of PZORG and PZL Sensors

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PATHFAST SERVICE MANUAL Chapter 5

PMT Shutter Open and Close Sensors (Rear of Instrument)

PSO

PSC

Fig 5-1-10 Location of PSO and PSC Sensors

Door Lock Sensor (Rear of Instrument)

DLS

Fig 5-1-11 Location of DLS Sensor

Waste Tip Sensor (Front of Instrument)

TIP

Fig 5-1-12 Location of TIP Sensor

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PATHFAST SERVICE MANUAL Chapter 5

Inter-lock Switch (Right Side of Instrument)

DOOR SW

Fig 5-1-13 Location of Door SW

Maintenance Switch (Inter-lock Release Switch: Left Side of Instrument)

MAINTENANCE SW

Fig 5-1-14 Location of Maintenance SW

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PATHFAST SERVICE MANUAL Chapter 5

5–1–3 Location of Electrical Components

Fig 5-1-15 Location of Electrical Components: Front

On the front of the instrument, the following components are located.

・ Indicator LED
・ LCD Unit with touch panel
・ START Switch
・ STOP Switch
・ LCD Brightness Adjustment Knob
・ Printer Unit
Behind LCD Unit with touch panel, the following printed circuit boards are located.
・ LCD I/F Board
・ LCD Inverter Board (for LCD back light)

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PATHFAST SERVICE MANUAL Chapter 5

Fig 5-1-16 Location of Electrical Components: Inner, Left Side

On the left side of the instrument, the following electrical components are located.
・ DC24V Power Supply (PS1)
・ DC5V Power Supply (PS2)
・ CPU Board
・ T-NET Board
・ EXT Board
・ Printer I/F Board
・ CF I/F Board
・ Motor Driver Board 1(T-BCD4020FUA3）
・ Motor Driver Board 2(T-BCD4020FUA3)

Fig 5-1-17 Location of Electrical Components: on Left Side Cover

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PATHFAST SERVICE MANUAL Chapter 5

On the inner left side cover, the following electrical components are located.
・ PSR Board
・ Temperature Controller 1
・ Temperature Controller 2
・ Temperature Controller 3
・ Electric Buzzer

Fig 5-1-18 Location of Electrical Components: Rear

On the rear of the instrument, the following electrical components are located.
・ Fan for Assay Area
・ Thermostat for Fan for Assay Area
・ Temperature Sensor Board
・ Fan for Electronics
・ PMT Shutter Unit
・ PMT Correction LED Unit
・ Inlet Filter with Fuse Holder
・ RS232C_I/F Connector
・ Ground Terminal
・ LAN Connector (Not Used)

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PATHFAST SERVICE MANUAL Chapter 5

5–1–4 Overview of Electronic Adjustment and Switch Setting

Electric adjustment is needed for the following two boards.

・ DC24VPower Supply
・ DC5VPower Supply

CPU Board, T-NET Board, EXT Board, Motor Driver Board1 and Motor Driver Board2
also contain the switches for function setting. However, you do not need to change
switch settings, because all switches are pre-set at factory before shipment.

Adjustment procedure and the description of the settings will be described in the later
part of this manual.

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5 – 2 Power Supply

5 – 2 – 1 General Description
- Electrical power is supplied through the inlet filter with fuse on the back
of the instrument by connecting the designated power cable to the wall
outlet.
- Power consumption of the instrument is AC100V-240V 50/60Hz 360VA.
- Chose the cable depending on the power used.
- Fuse is common for all voltages.
Power Used Power Cable Fuse
AC230V CEE type 4.0A T 250V
AC115V UL type 4.0A T 250V

- Main Power Switch is located on the left lower part of the instrument.
- AC100-240V is transformed into DC24V and DC5V by PS1 and PS2
respectively.
- DC24V power is used for driving stepping motors, fans, heaters, etc.
- DC5V power is used for electronics such as CPU Board, T-NET Board,
EXT Board, PMT Shutter Unit, Barcode reader, Printer, etc.
- Only parts that directly use AC power are PS1 and PS2.
- Sensors use DC24 V.

- Refer to the attached drawings for block diagrams.

- PATHFAST Block Diagram (1) (51204-210E**)
- PATHFAST Block Diagram (2) (51204-220E**)
- PATHFAST Block Diagram (3) (51204-230E**)

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PATHFAST SERVICE MANUAL Chapter 5

5–2–2 Locations of power supply related parts

Inlet Filter with Fuse Holder

Fig 5-2-1 Part Location-1

DC24V Power Supply

DC5V Power Supply

Main Switch

Fig 5-2-2 Part Location -2

5–2–3 Functions

Inlet Filter with Fuse Holder

- Supply power to the instrument
- Fuse holder is combined with noise filter.
-
Main Switch
- Switch for turning the instrument power On and Off.

DC24V Power Supply

- It supplies power for stepping motors, fans, heater units, etc.
- Sensors are also supplied from the power supply.
- LED on the power supply is On when AC power is applied.

DC5V Power Supply

- It supplies power for CPU Board, T-NET Board, EXT Board, PMT Shutter
Unit, Barcode reader, Printer, etc.

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Instructions for Safety

- Power supplied for stepping motors are shut automatically by the security
circuit when Front Cover is opened.
- The security circuit consists of Inter-lock Switch and Relay (RY1) on the
PSR board. RY1 shuts the power when Inter-lock Switch becomes OFF.
- If you want to operate stepping motors with Front Cover opened for
maintenance purpose, remove Left-side Cover and open the internal cover
to activate the Inter-lock Release Switch (called Maintenance Sw.) to
supply power to them.
- Pay enough attention not to get hurt, when working on the instrument
with Front Cover opened.

5–2–4 Servicing Procedure

<Procedure for Replacing Fuse>

1. Remove the fuse holder by prying it with a small flathead screw driver.

Fig 5-2-3 Replacing Fuse-1

Fuse

Fig 5-2-4 Replacing Fuse -2

2. Fuse to be used is common for the voltage between AC100 and AC240 volts.
3. Rating of the fuse is “4AT 250V”. Here “T” means “time-lag” type fuse.

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PATHFAST SERVICE MANUAL Chapter 5

<Procedure for Replacing Inlet Filter with Fuse Holder>

1. Remove two screws shown in the following picture using Phillips screw
driver (+).

Screws

Fig 5-2-5 Removing Inlet Filter with Fuse Holder -1

2. Remove three screws fixing the bracket for Inlet Filter with Fuse Holder,
using hex wrench, size 2.5mm.

Screws

Fig 5-2-6 Removing Inlet Filter with Fuse Holder -2

Fig 5-2-7 Removing Inlet Filter with Fuse Holder -3

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PATHFAST SERVICE MANUAL Chapter 5

<Procedure for Replacing Main Switch>

Remove two screws fixing the bracket and take the switch out.
(Tool: hex wrench, size 2.5mm)

Screws

Fig 5-2-8 Removing Main Switch -1

Fig 5-2-9 Removing Main Switch -2

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PATHFAST SERVICE MANUAL Chapter 5

<Procedure for Replacing DC24V Power Supply >

1. Remove 6 screws shown below which secure Left Inner Cover and then
remove it using hex wrench, 2.5mm.

Screws

Fig 5-2-10 Removing Left Inner Cover -1

Fig 5-2-11 Left Inner Cover

2. DC24V Power Supply is fixed with two screws as shown below. Use hex
wrench, 3mm.

Screws

Fig 5-2-12 Removing DC24V Power Supply -1

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PATHFAST SERVICE MANUAL Chapter 5

LED

Trimmer for voltage

adjustment

Fig 5-2-13 Removing DC24V Power Supply -2

Voltage adjustment for DC24V Power Supply

- Turn the trimmer screw of “VADJ” clockwise to increase the voltage using
Phillips screw driver (+).
- The voltage of DC24V shall be checked between check terminal of “24V-1”
(DC24Volt) and “GND(24)” (Ground) on PSR Board, which will be
described in later section.
- Specification for the voltage adjustment is 24.0V - 24.5V.

<Procedure for Replacing DC5V Power Supply>

DC5V Power Supply is fixed with two screws as shown below. Use hex
wrench, 3mm.

Screws

Fig 5-2-14 Removing DC5V Power Supply -1

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PATHFAST SERVICE MANUAL Chapter 5

LED

Trimmer for
voltage adjustment

Fig 5-2-15 Removing DC5V Power Supply -2

Voltage adjustment for DC5V Power Supply

- Turn the trimmer screw of “VADJ” clockwise to increase the voltage using
Phillips screw driver (+).
- The voltage of DC24V shall be checked between check terminal of “5V”
(DC5Volt) and “GND(5)” (Ground) on PSR Board, which will be described
in later section.
- Specification for the voltage adjustment is 5.0V – 5.1V.

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PATHFAST SERVICE MANUAL Chapter 5

<Procedure for Replacing PSR Board>

1. Remove the four screws shown below. Use hex wrench, 2.5mm.

Screws

Screws

Fig 5-2-16 Removing PSR Board

2. Power Thermistor is set between CN4 connector on PSR Board and the
cable connector.

CN4

Power Thermistor

Fig 5-2-17 Removing Power Thermistor

Fig 5-2-18 PSR Board

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PATHFAST SERVICE MANUAL Chapter 5

CN100 CN102 CN101 CN3

CN4
CN103

CN5

CN8

CN104
CN7

CN11
CN105

Fig 5-2-19 Connector location on PSR Board -1

CN1
CN109
CN6
CN108

CN9
CN106

CN107 CN12

CN10

CN110 Not CN111 Not

CN2
Used Used

Fig 5-2-20 Connector location on PSR Board -2

Details of PSR Board

- PSR Board distributes powers of DC24V and DC5V to other parts. It also
relays the signals from photo-sensors and drives heater units.
- Circuit configuration of DC24V power distributor.
(Except items described in heater unit section.)
-. LED1 :Indicates power input status of DC24V, it is On
when DC24V power is applied.
-. LED2 :Indicates power input status of DC24V, it is On
when DC24V power is applied and it becomes off
when Front Cover is opened.
-. F1 :Fuse for power to temperature controller, Sample
Recognition Sensor Board and Cooler Fan for
electrical parts.(min.125V 6.3AT)
-. CP1・CP2 :Check pins uses for measuring DC24V power.
(CP1=24V・CP2=GND)
-. F2 :Fuse for Motor Driver Board and Heaters.
（min.125V 6.3AT）
-. RY1 :Relay for security circuit (combined with Door
Inter-lock)

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PATHFAST SERVICE MANUAL Chapter 5

Circuit Description
- DC 24V power supplied through CN1 is sent to other parts through
connectors below via Fuse(F1).
- LED1 is On when the power is supplied to the board.
- Connect digital volt meter to check pins of CP1 and CP2 to measure the
voltage.
- Connector number and destination of the power
CN3 :Temperature Controller, Sample Recognition
Sensor Board and cooler fan for electric parts.
CN4 :Motor Driver Board, Cooler Fan for assay area and
PMT Solenoid.
- Fuse F1/F2 are prevention of over current.
- Relay RY1 cuts the DC24V power to Motor Controller Board in
combination with Inter-lock Switch when Front Cover is opened.
- CN8 is connected to Inter-Lock Release Switch. The Inter-lock Switch
is deactivated by this Inter-lock Release Switch when Left Side Cover is
removed.

Circuit Configuration of DC5V power distributor.

-. F3 :Fuse for CPU Board, T-NET Board and PMT Shutter Unit
（min.125V 6.3AT）
-. F4 :Fuse for EXT Board and Printer I/F Board.
(min.125V 6.3AT）
-. LED3 :DC5V Input indicator (On when power applied).
-. LED10 :DC5V Input indicator (On when power applied).
-. CP7, CP8 :Check Pins for DC5V. (CP7=5V, CP8=GND)
-. RY3 :Relay for supplying power to PMT Shutter Unit.

Circuit Description
- DC5V power coming through CN2 is supplied to its destination through the
below mentioned connector via Fuse (F3/4).
- LED3 and LED10 are On when the power is supplied.
- Connect digital voltmeter between CP7 and CP8 to check the voltage
supplied.
- Connector and destination of DC5V.
-. CN11 :CPU Board, T-NET Board, PMT Shutter Unit,
EXT Board and Printer I/F Board.
- Relay RY3 supplies DC5V to PMT Shutter Unit when activated by the
signal of PMT5VON from connector CN9.

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PATHFAST SERVICE MANUAL Chapter 5

5–2–5 Wiring
Refer to the drawings listed and attached.
・ PATHFAST Wiring Diagram (1) (51204-310E**)
・ PATHFAST Wiring Diagram (2) (51204-320E**)
・ PATHFAST Wiring Diagram (3) (51204-330E**)
・ PATHFAST Wiring Diagram (4) (51204-340E**)
・ PATHFAST Wiring Diagram (5) (51204-350E**)
・ PATHFAST Wiring Diagram (6) (51204-360E**)
・ PATHFAST Wiring Diagram (7) (51204-370E**)
・ PATHFAST Wiring Diagram (8) (51204-380E**)
・ PATHFAST Wiring Diagram (9) (51204-390E**)
・ PATHFAST Wiring Diagram (10) (51204-400E**)
・ PATHFAST Wiring Diagram (11) (51204-410E**)
・ PATHFAST Wiring Diagram (12) (51204-420E**)
・ PATHFAST Wiring Diagram (13) (51204-430E**)
・ PATHFAST Wiring Diagram (14) (51204-440E**)

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PATHFAST SERVICE MANUAL Chapter 5

5–3 Heater Units

5–3–1 General Description

-. PATHFAST has three heater units.

-. They are controlled at the following temperature.
-. Heat Block L :37.5℃±0.3℃. Controlled by Temperature Controller1.
-. Heat Block S :65.0℃±2.0℃ Controlled by Temperature Controller2.
-. Tip Heater :35.0℃±0.5℃ Controlled by Temperature Controller3.

Refer to the attached wiring block diagram.

・ PATHFAST Block Diagram (2) (51204-220E**)
・
5–3–2 Location of each part
Tip Heater

Heat Block S

Heat Block L

Fig 5-3-1 Location of Heat Blocks

PSR Board Temperature

Controller 3/2/1

Fig 5-3-2 Location of Temperature Controller and PSR Board

5–3–3 Functions
- Heater unit consists of Heat Block, PSR Board and Temperature
Controller.
- Heat Block consists of Heater, Thermo-couple and Thermal Fuse.
- Rated power of one heater is DC24V 20W. Heat Block L employs two
heaters the others employ one.

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PATHFAST SERVICE MANUAL Chapter 5

- Thermal Fuse blows at 102℃.

- If one of the thermal fuses is blown, power to all the heaters is shut down.
- Thermo-couple is K-Type.
- FET1/FET2/FET4 on PSR Board are elements to make the heaters On or
Off.
- Temperature Controllers are driven by DC24V power.
- Information of set temperature is sent to Temperature Controller by serial
communication (RS485) through connector CN17 on CPU Board.
- Communication is bi-directional.
- Temperature of heat block is monitored by Temperature Controller and it
reports to CPU Board.
- On the Temperature Controller, the numeric number shown in upper case
is the current temperature and the lower is the set temperature.

Details of PSR Board

- On the PSR Board, controllers of heat blocks are located. In the following,
details of the controller are described.
- Circuit Configuration
・ FET1 : FET to make Heater1 On or Off
・ FET2 : FET to make Heater2 On or Off
・ FET4 : FET to make Heater3 On or Off
・ RY2 : Relay for Security Circuit
(prevent over heating)
・ LED5 : LED to indicate Heater1 is On or Off
・ LED6 : LED to indicate Heater2 is On or Off
・ LED11 : LED to indicate Heater3 is On or Off
・ CP4 : Check Pin to check Heater1 is On or Off
・ CP5 : Check Pin to check Heater2 is On or Off
・ CP11 : Check Pin to check Heater3 is On or Off
・ CP8 : Check Pin for GND(ground)
・ LED8 : LED to indicate Security Circuit operation
(prevent over heating)
- Circuit Description
・ Connector CN5 is connected to heater. Connector CN7 is
connected to output of Heater On/Off signal from Temperature
Controller. Connector CN6 is connected to Thermal Fuse.
・ Relay RY2 is activated when Thermal Fuse becomes hot (it
becomes Off at more than 102 deg C) to shut the power of DC24V
going to CN5. LED8 and other indicator LEDs are On in normal
condition and go Off when Thermal Fuse becomes Off.

5–3–4 Servicing Procedure

- Replacing procedure for Heater Blocks are described in Mechanical Part of
this manual.

5–3–5 Cable Wiring

Refer to the below mentioned drawings for wiring attached to this manual.
・ PATHFAST Wiring Diagram (9) (51204-390E**)
・ PATHFAST Wiring Diagram (10) (51204-400E**)

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PATHFAST SERVICE MANUAL Chapter 5

5–4 FAN

5–4–1 General Description

- PATHFAST has FAN1 for assay area and FAN2 for electronic parts
enclosure.
- FAN1 is activated when the temperature in assay area (called as
Chamber) goes upper than 28℃+/-1℃.
- FAN2 is operated while AC power is supplied.

・ Refer to the attached Block Diagram for wiring.

PATHFAST Block Diagram (2) (51204-220E**)

5–4–2 Part Locations

Fan for Electronic Parts Enclosure

Fan for Assay Area

Fig 5-4-1 FAN Locations

Thermostat

Fig 5-4-2 Thermostat

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PATHFAST SERVICE MANUAL Chapter 5

5–5 System Controller

5 – 5 – 1 General Description
- PATHFAST is controlled by CPU Board, T-NET Board and EXT board.
- OS, Firmware and Protocols are written in CF card.
- Peripheral devices are LCD Unit with Touch Panel, two Motor Driver
Boards, PSR Board, Temperature Controller, Printer Unit, built-in
Barcode Reader and Handheld Barcode reader.
- PMT Shutter Unit detects chemi-luminescent light.
- System Controller consists of the following boards and units.

Name Type
CPU Board SACS-CPU
T-NET Board SACS-TCOM
EXT Board SACS-ST100-EXT
LCD Display with Touch Panel DTM-057KS13
LCD I/F Board LCD I/F
Inverter Board (for LCD Back Light) CXA-L0612A-VJL
Motor Driver Board 1 ED-9380B T-BCD4020FUA3
Motor Driver Board 2 ED-9380B T-BCD4020FUA3
PSR Board PSR I/O
Temperature Controller 1 TTM-002-P-AM-24
Temperature Controller 2 TTM-002-P-AM-24
Temperature Controller 3 TTM-002-P-AM-24
TEMP PCB Assy Temp PCB
Printer I/F Board IFH002-01BK
CF I/F Board CF I/F
Barcode Reader TC-1100-1100
PMT Shutter Unit H7155
PMT Calibration Unit 51207-33 (Unit No.)
Handheld Barcode Reader Gryphon D100
Refer to the below mentioned drawings for wirings, attached in Chapter 11.
・ PATHFAST Block Diagram (1) (51204-210E**)
・ PATHFAST Block Diagram (2) (51204-220E**)
・ PATHFAST Block Diagram (3) (51204-230E**)

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PATHFAST SERVICE MANUAL Chapter 5

5–5–2 Board/Unit Location

Each board or unit is located as follows.

EXT Board T-NET Board

CPU Board

Fig 5-5-1 System Controller -1

Main boards of System Controller are located in three layer, EXT board at the
top, T-NET Board in the middle and CPU Board at the bottom.

LCD Display with Touch Panel

Fig 5-5-2 System Controller -2

On the back of LCD Unit with Touch Panel, LCD I/F Board and Inverter Board
for LCD Back Light are located.

Motor Driver Board 2

Motor Driver Board 1

Fig 5-5-3 System Controller-3

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PATHFAST SERVICE MANUAL Chapter 5

The two Motor Driver Boards are the same, but the function switches (Dip
Switches) and motor driving current are differently set. Check those settings
before replacing the board.
The details of settings are described in later section.

PSR Board

Fig 5-5-4 System Controller -4

Temperature Controller
3/2/1

Fig 5-5-5 System Controller -5

Refer to the “Heater Unit” section for details.

TEMP PCB Assy

Fig 5-5-6 System Controller -6

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PATHFAST SERVICE MANUAL Chapter 5

CF I/F Board

Printer I/F Board

Fig 5-5-7 System Controller -7

Barcode Reader

PMT Shutter Unit

Fig 5-5-8 System Controller -8

PMT Calibration Unit

Fig 5-5-9 System Controller -9

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PATHFAST SERVICE MANUAL Chapter 5

Handheld Barcode Reader

Fig 5-5-10 System Controller -10

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PATHFAST SERVICE MANUAL Chapter 5

5–5–3 Details of Function

In the followings, details of each part are described.

<CPU Board>
- Controls whole PATHFAST system.
- CPU board controls whole PATHFAST system by OS, Firmware and
Protocols in CF card.
- CF card shall be set in CF Card I/F Board.
- Power supplied to the board is DC5V and it is converted into 3.3.VDC on
CPU Board to be used by LSIs and Memorys on CPU Board and peripheral
devices.
- Motors are driven by two 3-Axes Motor Driver Board and T-NET Board
connected with bus CN5/CN6 connectors.
- I/O control is performed by PSR Board through T-NET Board.
- LCD Unit with Touch Panel is controlled by CPU Board through LCD I/F
Board connected to CN5 on CPU Board.
- Heater Units are controlled by three Temperature Controllers connected to
CPU Board through CN17 (RS485 I/F) and heater driver circuit on PSR
Board.
- Temp Board used to monitor the temperature in assay area is connected to
CPU Board through CN10 on CPU Board.
- Printer is controlled by CPU through Printer I/F Board connected through
CN6 on CPU Board.
- Handheld Barcode Reader is controlled by CPU through CN7 on CPU
Board.
- PMT Shutter Unit is controlled by CPU through PSR Board setting power
to PMT On or Off, and the data from PMT is collected through serial
communication port of connecter CN1 on EXT Board.
- LED Unit for PMT Correction is connected to CN108 on PSR Board.

- CPU Board has four LEDs to show the operating status of CPU Board as
follows.
- LED1 :On when data of F-ROM is under the process of rewritten.
- LED2 :On while power is supplied to CPU Board.
- LED3 :On while LAN port is in normal operation.
- LED4 :On while LAN port is in normal operation.

Note1): Do not attempt to change settings on CPU Board, because function

setting switches are already set at factory.
Note2): Do not place the removed CPU Board on the conductive material
such as metal sheet or aluminum foil, because it carries Lithium
Battery on board. Also do not wrap CPU Board with such
conductive materials and wrap it with anti-static film or packing to
avoid short circuit of Lithium Battery.

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PATHFAST SERVICE MANUAL Chapter 5

<T-NET Board>
- It performs motor control and I/O control based on the command
parameters from CPU Board.
- It is connected with CPU Board with bus connector.
- Motors are controlled through two 3-Axes Motor Driver Boards.
- Connector CN13 on T-NET Board is connected to two 3-Axes Motor Driver
Boards in multi-drop form. Communication is based on RS485.
- I/O control is made through PSR Board.
- Connectors of CN7, CN8 and CN12 on T-NET Board are connected PSR
Board.
- Connectors CN7 and CN8 are for output control.
- Connector CN12 is for input of sensor signals.

- T-NET Board has three LEDs to indicate operation status as follows.

- LED4 :On while normal communication is kept between T-NET
Board and 3-Axes Motor Driver Board.
- LED8 :On when normal communication is lost between T-NET
Board and 3-Axes Motor Driver Board.
- LED9 :On while power is supplied to T-NET Board.

Note1): Do not attempt to change settings on T-NET Board, because

function setting switches are already set at factory.

<EXT Board>
- It controls Barcode Reader, Handheld Barcode Reader, Printer, PMT
Shutter Unit and RS232C port for communicating with external devices
based on the command parameters from CPU Board.
- Command parameters are exchanged by serial communication between
CPU board and EXT Board.
- CN4 on EXT Board is connected to CN19 of CPU Board.
- Barcode Reader is controlled through connector CN7 on EXT Board.
- Handheld Barcode Reader is controlled through CN8 on EXT Board.
- Printer is controlled by Printer I/F Board connected to CN6 on EXT Board.
- PMT Shutter Unit is controlled through CN5 on EXT Board.
- RS232C port for communicating with external devices is controlled
through CN6 on EXT Board.

Note1): Do not attempt to change settings on EXT Board, because function

setting switches are already set at factory.

< LCD Display with Touch Panel>

- It consists of touch panel for instrument operation and monochromatic
LCD display.
- On the back of the unit, LCD I/F Board and Inverter Board for LCD
Backlight.
- LCD Brightness Adjustment Knob is located behind Printer Cover.

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PATHFAST SERVICE MANUAL Chapter 5

<Motor Driver Board>

- Two Motor Driver Boards are used,.
- Motor Driver Board-1 controls the motors for Y-Axis, Z-Axis and P-Axis.
- Motor Driver Board-2 controls the motors for M-Axis, PMTX-Axis and
PMTZ-Axis.
- All End Limit Sensors for the motors are connected to these boards.
- Command Parameters from CPU Board are transferred to Motor Driver
boards via T-NET Board.
- DC24V power is supplied to these boards.
- DC24V power is shut off by Interlock Switch(Door Switch), when Front
Cover is opened.

- On these boards, 7 LEDs are prepared to indicate the status for each
board.
- PWR : DC24V power indicator, On when power is supplied.
- ERR : On when communication error occurred for Y-Axis (or
M-Axis).
- ERR2 : On when communication error occurred for Z-Axis (or
PMTX-Axis).
- ERR3 : On when communication error occurred for P-Axis (or
PMTZ-Axis).
- RUN1 : On when normal communication is kept for Y-Axis (or
M-Axis).
- RUN2 : On when normal communication is kept for Z-Axis (or
PMTX-Axis).
- RUN3 : On when normal communication is kept for P-Axis (or
PMTZ-Axis).

Note1) Two Motor Driver Boards are identical and make sure that the
function setting switches are for those boards before replacing boards.
Settings of those switches is described in the later part.

<PSR Board>
In the followings other functions than described before (power distribution and
heater driver) are explained.

- I/O control function of PSR Board is as follows.

- ZB Sensor mask : LED116 ZAC is On to show ZB senor is invalid.
(Used when taking tips.)
- PMT Shutter ON : LED117 Sopen is On to show PMT Shutter is
open.
- Buzzer : LED118 BZ is On to show Buzzer is ON.
- Power for PMT Shutter Unit : LED119 PMTpow is On to show power
for PMT Shutter Unit is ON.
- Front Panel LED (Green) is ON
: LED124 PLEDG is On showing green LED is
ON.
- Front Panel LED (Red) is ON
: LED125 PLEDR is On showing red LED is ON.

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PATHFAST SERVICE MANUAL Chapter 5

- PMT Calibration LED is ON

: LED126 PMTLED is On showing PMT
Calibration LED is ON.
- Front Cover locked : LED127 CLS is On showing Front Cover is locked.
- Sensor signals are checked and shown as follows.
- ZB Sensor : LED100 ZB is Off showing ZB Sensor is activated.
- PSC Sensor : LED101 PSC is On showing PSC Sensor is
activated. (PMT Shutter is closed: 1%)
- PSO Sensor : LED102 PSO is On showing PSO Sensor is
activated. (PMT Shutter is opened: 100%)
- PZL Sensor : LED103 PLS is On showing PZL Sensor is activated.
(PMT Shutter Unit is lowered to the measuring
position)
- START Switch : LED104 Runsw is On showing START switch is
activated.
- STOP Switch : LED105 Stosw is On showing STOP switch is
activated.
- DSL Sensor : LED106 SolL is On showing DSL sensor is activated.
(Front Cover is locked.)
- TIP Sensor : LED107 Tips is On showing Tip senor is activated.
(Used tip is detected.)

<Temperature Controller 1/2/3>

- Refer to the previous section for Heater Unit.

<TEMP PCB Assy >

- This board monitors the temperature in assay area.
- CPU gets the temperature through CN10 on CPU Board.

<Printer I/F Board>

- This board controls built-in Printer.
- It is controlled through CN6 on EXT Board.

<CF I/F Board>

- It is an interfacing board with CF Card slot.
- Connected to CN2 on CPU Board.
<Barcode Reader>
- It is a built-in barcode reader to read barcodes on reagent cartridges.
- Connected to CN7 on CPU Board.
<PMT Shutter Unit>
- It is a detector unit to get luminescent light in detection well of reagent
cartridge.
- Connected to CN1 on EXT Board.
- Power for PMT Shutter Unit is supplied through CN12 on PSR Board from
DC5V supply.
Note) The PMT Shutter Unit includes a photomultiplier tube to detect very
small light signals and it is so sensitive that it may be damaged if it
is exposed to even room light. When removing and replacing PMT
Shutter Unit, be sure that the instrument power is turned off. Also
disconnect CN12 on PSR Board to work on other part of the
instrument with instrument covers removed.

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PATHFAST SERVICE MANUAL Chapter 5

<PMT Calibration Unit>

- It is a LED Unit to calibrate PMT Shutter Unit.
- Connected to CN108 on PSR Board.

<Handheld Barcode Reader>

- It is an external barcode reader hooked up with the instrument.
- It gathers information of sample ID or operator ID from their barcodes.
- Connected to CN8 on CPU Board through RS232C port.

5–5–4 Servicing Procedure

<EXT Board>
1. Disconnect CN1 (BNC connector). It is a special connector with round shape.
Referring to the picture below, rotate the connector counter clockwise to
unlock and pull out it.

Fig 5-5-11 Removing CN1 -1

Fig 5-5-12 Removing CN1 -2

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PATHFAST SERVICE MANUAL Chapter 5

2. Remove four screws in the following picture to pull out the board. Use hex
wrench, 2.5mm.

Screws

Fig 5-5-13 Removing EXT Board

Fig 5-5-14 EXT Board

CN3 CN15
Not Used Not Used
CN8

CN7

CN5

CN4
CN1
CN14

Fig 5-5-15 Connector Location on EXT Board

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PATHFAST SERVICE MANUAL Chapter 5

<T-NET Board>
1. Remove two hex supports and two screws to purl out the board. Use hex
wrench, 2.5mm.
Note) Do not remove the two supports shown by yellow arrows in the
following picture. These are fixed with screws on T-NET Board to
support EXT Board.

Hex Supports Screws

Fig 5-5-16 Removing T-NET Board

Hex Supports

Fig 5-5-17 T-NET Board

CN13

CN17 CN12

CN18 CN8 CN7

Fig 5-5-18 Connectors on T-NET Board

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PATHFAST SERVICE MANUAL Chapter 5

<CPU Board>
1. Remove four hex supports to pull out the board. Use hex wrench, 2.5mm.

Fig 5-5-19 CPU Board

Lithium Battery

Fig 5-5-20 CPU Board

Note) Because it carries a Lithium Battery on board, do not place the removed
CPU Board on the conductive material such as a metal sheet or
aluminum foil. Also do not wrap the CPU Boars with such conductive
materials. Wrap it anti-static film or packing to avoid damaging the
board.

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PATHFAST SERVICE MANUAL Chapter 5

CN5 CN6 CN7 CN20

CN12
Not Used

CN18
CN11 Not Used

CN10
Not Used

CN9
CN19 CN17 CN2 CN4

Fig 5-5-21 Connector location on CPU Board

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PATHFAST SERVICE MANUAL Chapter 5

<LCD Display with Touch Panel>

1. Remove three screws securing the metallic left front cover, referring to the
picture below. Use hex wrench, 2.5mm.

Screws

Fig 5-5-22 Removing LCD Display with Touch Panel -1

2. Remove two screws on the opposite side of the same cover, referring the
picture below

Screws

Fig 5-5-23 Removing LCD Display with Touch Panel -2

3. Remove two screws on the front of the cover, referring the picture below.
Use Phillips screw driver (+).

Screws

Fig 5-5-24 Removing LCD Display with Touch Panel -3

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PATHFAST SERVICE MANUAL Chapter 5

4. Remove the operation panel cover, pulling it toward you.

Screws

Fig 5-5-25 Removing LCD Unit -4

5. Remove three screws securing internal cover on the left side. Refer to the
picture below. Use hex wrench, 2.5mm.

Screws

Fig 5-5-26 Removing LCD Unit -5

6. Referring the picture below, remove three screws on the right hand side and
remove the internal cover by pulling it toward you. Use hex wrench, 2.5mm.

Screws

Fig 5-5-27 Removing LCD Unit -6

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PATHFAST SERVICE MANUAL Chapter 5

LED Ass’y LCD I/F Board

Inverter Board for LCD

Back Light

START STOP
Switch Switch

Fig 5-5-28 Behind LCD Display with Touch Panel

<LED Ass’y>
Remove the agraffe fixing LED, using small driver.

Fig 5-5-29 Removing LED Ass’y

Fig 5-5-30 LED Ass’y

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PATHFAST SERVICE MANUAL Chapter 5

<LCD I/F Board>

1. Release the connector lock using a small driver and pull out the flexible
cable.

Fig 5-5-31 Removing LCD I/F Board -1

Connector lock is
released.

Fig 5-5-32 Removing LCD I/F Board -2

2. Pull out the other flexible cable which does not have connector lock.
3. Remove other connectors from wire harness.
4. Remove four screws fixing LCD I/F Board.

Fig 5-5-33 Removing LCD I/F Board -3

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PATHFAST SERVICE MANUAL Chapter 5

<Inverter Board (for LCD Back Light)>

Remove two screws in the following picture. Use hex wrench, 2mm.

Screws

Fig 5-5-34 Removing Inverter Board (for LCD Back Light)

Fig 5-5-35 Inverter Board (for LCD Back Light)

<LCD Display with Touch Panel>

Remove four screws shown in the picture below. Use hex wrench, 2.5mm.

Screws

Fig 5-5-36 Removing LCD Unit with Touch Panel

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Fig 5-5-37 LCD Unit with Touch Panel

<START Switch/ STOP Switch>

1. These switches are made of two parts. Rotate the lock lever counter
clockwise to release lock and to make the switch separated into two parts.

Lock Lever

Fig 5-5-38 Removing START/STOP Switches -1

2. Rotate the plastic screw of the switch counter clockwise to remove.

Plastic Screw

Fig 5-5-39 Removing START/STOP Switches -2

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Fig 5-5-40 START/STOP Switch

3. Assemble the switch in the reverse procedure. When fixing the switch,
follow the procedure below to assure the switch button to move
smoothly in and out without interfered by the housing.
Perform the adjustment with Operation Panel installed onto the
instrument.

4. First install the switch body in the reverse procedure of disassembling.

Do not put the rest of the switch with cables at this moment.

5. To tighten the Plastic Screw, use the tool shown below.

6. When assembling Start Switch, for example, tighten the Plastic Screw
with the switch button pressed.

7. Check if the switch button moves in and out with no obstruction, by

rotating it or pushing one part of the switch button.

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Rotate switch button The button

8. If the switch button does not move in and out smoothly in step 7,
re-tighten the Plastic Screw as described in step 6 to find the location
where the switch button moves in and out smoothly.
9. When adjustment completed, attach the rest of the switch with cables.

< Motor Driver Boards>

Remove four fixing screws for each board, using hex wrench, 2.5mm.

Motor Driver Board -2

Motor Driver Board -1

Fig 5-5-41 Removing Motor Driver Boards

Fig 5-5-42 3-Axes Motor Driver Board

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<Temperature Controller>

Temperature Controller-3

Temperature Controller-2

Temperature Controller-1

Fig 5-5-43 Temperature Controllers

1. Remove the cables behind Temperature Controllers, using a small driver.

Fig 5-5-44 Removing Temperature Controller -1

2. Widen the plastic fixture to pull out Temperature Controller.

Plastic Fixture

Fig 5-5-45 Removing Temperature Controller -1

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Fig 5-5-46 Temperature Controller

3. After replacing the Temperature Controller, adjust the offset value

following the procedure described in 4-7-2 Replacing Heater Unit in
Chapter 4.
Be sure the room temperature is within the required range for the work.

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<TEMP PCB Assy>

Remove two screws to remove the board, using hex wrench, 2.5mm.

Screws

Fig 5-5-47 Removing TEMP PCB Assy -1

Fig 5-5-48 TEMP PCB Assy

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<Printer I/F Board>

1. Printer I/F Board is mounted on Printer Unit. Remove the unit by

removing three screws. CF Card I/F Board is also mounted on the same
unit. Use hex wrench, 2.5mm.

Screws

Fig 5-5-49 Removing Printer Unit -1

Screws

Fig 5-5-50 Removing Printer Unit -2

2. Remove four screws to remove Printer I/F Board. Use hex wrench, 2.5mm.

Fig 5-5-51 Printer Unit

3. Release the connector lock using a small driver and pull out the flexible

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cable as described for LCD I/F Board.

Fig 5-5-52 Printer Unit

Fig 5-5-53 Printer I/F Board

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PATHFAST SERVICE MANUAL Chapter 5

<CF I/F Board>

1. After removing Printer I/F Board, remove two screws to remove the metal
plate. Use hex wrench, 2.5mm.

Screws

Fig 5-5-54 Removing CF Card I/F Board-1

2. Next, remove two screws securing the board. Use hex wrench, 2.5mm.

Fig 5-5-55 Removing CF Card I/F Board-2

Fig 5-5-56 CF Card I/F Board

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<Printer >
1. Remove the metal plate by removing two fixing screws. Use hex wrench,
2.5mm.

Screws

Fig 5-5-57 Removing Printer-1

2. Open the cover by pressing the lock lever.

Lock Lever

Fig 5-5-58 Removing Printer -2

3. Take out Printer by removing two screws. Use small Phillips driver (+).

Screws

Fig 5-5-59 Removing Printer -3

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PATHFAST SERVICE MANUAL Chapter 5

Fig 5-5-60 Printer

<PMT Shutter Unit>

Refer to 4-11-2 Replacing PMT Shutter Unit in Chapter 4 for replacing the
unit.

<PMT Calibration Unit>

Remove PMT Calibration Unit by removing the snap ring.

Fig 5-5-61 Removing PMT Calibration Unit -1

Refer to the picture below for snap ring and the remover.

Fig 5-5-62 Removing PMT Calibration Unit-2

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Fig 5-5-63 PMT Calibration Unit

When you replaced PMT Calibration Unit, you need to adjust/edit H Count
and H/L Ratio of PMT. Follow the procedure below to check and adjust them.

[Procedure]

1. Open the left side cover and adjust the Trimmer (VR100) on PSR Board
at the central position of its rotation.

VR100)
Trimmer

2. Turn the instrument power on with Maintenance CF installed.

3. Select PMT OPERATION in MAINTENANCE MENU screen below.

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MAINTENANCE MENU

4. Select NEXT in Navi Area of the screen below to show PMT

OPERATION 2 screen.
PMT OPERATION

5. Select LED MEASUREMENT in PMT OPERATION 2 screen to

measure PMT Hi Count.
Adjust Trimmer (VR100) on PSR Board so that Hi Count falls in the
range of 1.0 +/- 0.1 million counts. Measure the count by selecting
LED MEASUREMENT every time you move the trimmer.

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PMT OPERATION ２

Hi Count
measured

6. When the Hi Count fell in the specified range, record the H/L Ratio to
edit H/L Ratio following the procedure describe in 7 – 6 Editing H/L
Ratio for PMT. Then check that the Dark Count is in the specified
range by following the procedure in 10 – 6 PMT Check of Chapter 10.

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5–5–5 Board Settings

For those printed circuit boards supplied as spare parts, switches are set as
required and tested at the factory before shipment.
But carefully check the settings of switches on Motor Driver Board before
replacing it. This is because that Motor Driver Board-1 and -2 are identical
excepting switch settings.

Details of switch settings for Motor Driver board follow.

< Motor Driver board-1> driver for Y-, Z-, and P-Axes
Dip Switch (DSW1)
DSW1-1: OFF 1st Axis Resolution-1 (1/8step)
DSW1-2: OFF 1st Axis Resolution-2
DSW1-3: OFF 1st Axis Resolution-3
DSW1-4: OFF 1st Axis, Comm. Speed (2Mbps)

Dip Switch (DSW2)

DSW2-1: OFF
DSW2-2: OFF
DSW2-3: OFF
DSW2-4: OFF
DSW2-5: OFF
DSW2-6: OFF
DSW2-7: OFF
DSW2-8: OFF

Dip Switch (DSW3)

DSW3-1: OFF 2nd Axis Resolution (1/8step)
DSW3-2: OFF 2nd Axis Resolution
DSW3-3: OFF 2nd Axis Resolution
DSW3-4: OFF 2nd Axis, Comm. Speed (2Mbps)

Dip Switch (DSW4)

DSW4-1: OFF
DSW4-2: OFF
DSW4-3: OFF
DSW4-4: OFF
DSW4-5: OFF
DSW4-6: ON
DSW4-7: OFF
DSW4-8: OFF

Dip Switch (DSW5)

DSW5-1: OFF 3rd Axis Resolution (1/8step)
DSW5-2: OFF 3rd Axis Resolution
DSW5-3: OFF 3rd Axis Resolution
DSW5-4: OFF 3rd Axis, Comm. Speed (2Mbps)

Dip Switch (DSW6)

DSW6-1: OFF
DSW6-2: OFF

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DSW6-3: OFF
DSW6-4: OFF
DSW6-5: OFF
DSW6-6: OFF
DSW6-7: ON
DSW6-8: OFF

JP1(Jumper Connector) : 1, 2 Open

2 JP1 1

No terminating resistance

JP2(Jumper Connector) : 1, 2 Closed

3 JP2 1

1st Axis Current Down Software controlled

JP3(Jumper Connector) : 1, 2 Closed

3 JP2 1

2nd Axis Current Down Software controlled

JP4(Jumper Connector) : 1, 2 Closed

3 JP2 1

3rd Axis Current Down Software controlled

< Motor Driver board-2> driver for M-, PMTX-, and PMTZ-Axes
Dip Switch (DSW1)
DSW1-1: OFF 1st Axis Resolution-1 (1/8step)
DSW1-2: OFF 1st Axis Resolution
DSW1-3: OFF 1st Axis Resolution
DSW1-4: OFF 1st Axis, Comm. Speed (2Mbps)

Dip Switch (DSW2)

DSW2-1: OFF
DSW2-2: OFF
DSW2-3: OFF
DSW2-4: OFF
DSW2-5: OFF
DSW2-6: ON
DSW2-7: ON
DSW2-8: OFF

Dip Switch (DSW3)

DSW3-1: OFF 2nd Axis Resolution (1/8step)
DSW3-2: OFF 2nd Axis Resolution
DSW3-3: OFF 2nd Axis Resolution
DSW3-4: OFF 2nd Axis, Comm. Speed (2Mbps)

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Dip Switch (DSW4)

DSW4-1: OFF
DSW4-2: OFF
DSW4-3: OFF
DSW4-4: OFF
DSW4-5: OFF
DSW4-6: OFF
DSW4-7: OFF
DSW4-8: ON

Dip Switch (DSW5)

DSW5-1: OFF 3rd Axis Resolution (1/8step)
DSW5-2: OFF 3rd Axis Resolution
DSW5-3: OFF 3rd Axis Resolution
DSW5-4: OFF 3rd Axis, Comm. Speed (2Mbps)

Dip Switch (DSW6)

DSW6-1: OFF
DSW6-2: OFF
DSW6-3: OFF
DSW6-4: OFF
DSW6-5: OFF
DSW6-6: ON
DSW6-7: OFF
DSW6-8: ON

JP1(Jumper Connector) : 1, 2 Closed

2 JP1 1

With Terminating resistance

JP2(Jumper Connector) : 1, 2 Closed

3 JP2 1

1st Axis Current Down Software controlled

JP3(Jumper Connector) : 1, 2 Closed

3 JP2 1

2nd Axis Current Down Software controlled

JP4(Jumper Connector) : 1, 2 Closed

3 JP2 1

3rd Axis Current Down Software controlled

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Chapter 6 Software Configuration

This chapter provides an overview of the configuration and control flow of PATHFAST
software.Also procedures for software upgrade, data backup, etc. are also explained in
this chapter.

6-1 Block Diagram of Hardware/Electrical Parts

Following is a description of the hardware/electrical parts controlled by the software.

Motor Motor LCD Touch SD(*1)- CT(*2)-

Controller Controller 320x240 Panel Sensor Sensor
1 2

R S- 485

CPU Board
T-Net Board A nalog
Vr4181
G8014 Compact
Flash
R S-485
Flash:16MB
RAM:16MB
TC(*3)1 TC2 TC3
I/O Board (Front) (Back) (Tip)

RS-23 2C LAN
LAN

R S-232C
Sensors
Expansion Internal BCR
R S-232C
Board Handy BCR
H8
PMT Firmware
5V R S-232C
Unit Printer
Pulse

R S- 232C
HOST ( *1) SD : S am ple descr im ina tor
( *2) CT : C ha nber Temp er atu re
( *3) TC : T he rmo Contr ol ler

<CPU Board>

CPU Board carries CPU of Vr4181 from NEC , 16MB of Flash Memory and 16MB

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PATHFAST SERVICE MANUAL Chapter 6

of RAM. RAM is used when executing OS or application software. Programs (BOOT,

BIOS) used in boot up of PATHFAST and the image of OS are stored in Flash
Memory. The rest of the memory is used for storing instrument data, or as a
temporary storage area for data in data backup or software version update
operation.
This board also carries functions of LCD Controller, Touch Panel interface and A/D
converter for Sample Recognition Sensor and Temperature in assay area.

The Compact Flash card holds the operating system (OS) and applications for
controlling PATHFAST. The CF card slot is located on the front part of the
instrument to make it easy to access when performing software upgrade operations.
The CF card slot is connected to the CPU by bus.

The CPU Board has two serial ports, one works as an RS485 to control three
temperature controllers and the other communicates with the EXT Board as
RS232C (TTL). The board also has a LAN port which is used for development
purposes.

<EXT Board>

The CPU of H8 is located on the EXT Board controls the built-in Barcode Reader,
Handheld Barcode Reader, Host communication and Printer by its firmware.
Also, the EXT Board has a programmable digital counter that counts pulses from
the PMT Unit. It is controlled by CPU Board and the count data is sent to CPU
Board.

<Motor Control>

The T-NET Board, connected to CPU Board, has communication ports that
communicate with motor controllers and the I/O port.
RS485 is used to communicate with two motor controllers at one time.
The I/O port is connected with I/O Board (PSR Board) and further connected to
sensors or actuators after signal level is converted.

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PATHFAST SERVICE MANUAL Chapter 6

6-2 Software Configuration

PATHFAST uses the Linux Operating System.

After an instrument is turned on, “
BOOT”program is read from Flash memory of the
CPU board, then the BOOT program activates “
BIOS”program. The BIOS loads the
operating system into RAM, after which the operating system takes control.
At this point the application programs are loaded.

6-3 Structure of Applications

The application consists of several independent modules.

On the EXT Board, firmware mediates communication between several devices and
the CPU.

CPU Board
LCD, Touch Panel
VR-Linux (OS)
PFMain( Main)
Motor, I/O
Window control Motor x 6
T-Net Board
Motor ctrl.
I/O
I/O
Inter module comumnication

PFCalc(Ca lculation) (SW,Sensors)

mtesky(H/W Control)
- script Interpleter Termo1～3, Chanber termo
- Motor Control
- IO(SW, Sensor) Expansion Board
PMT
ExtBoard
Firmware Interna l Ba rcod e Read er
HostIF(ASTM-Host)
Ext. board Handy Barco de Reader
SEA(Event watch)
Printer
BIOS
Host Port

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PATHFAST SERVICE MANUAL Chapter 6

An abstraction of the modules follows.

Module name Abstraction

PFMain Controls LCD and touch panel User Interfaces
Master of all functions.
PFCalc Calculates calibration curves and concentrations at request of
PFMain.
mtesky Controls motor and I/O usually at request of PFMain.
Also contains functions that interpret and perform scripts or
protocols.
ExtBoard Communicates by serial connection with the following devices
via an Extension board usually at request of PFMain.
- PMT
- Internal barcode reader
- External barcode reader
- Printer
- External communication(HOST)
HostIF Translates PATHFAST data to ASTM protocol format, then
communicates with HOST usually at request of PFMain.
Rely EXT Board on the actual communication with HOST
SEA Surveys events by request from other modules, then inform
when states are changed.

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PATHFAST SERVICE MANUAL Chapter 6

6-4 Control Process and Files

Control flow and files

PFMain
In stru men t depend ing
LCD, in iti alizati on of tou ch pan el
info rmati on except fo r
Screen co ntro l
posi tio n data , Iterm
C alcu la tio n, user infoerfa ces
parameters, protocol
parameters
Samp le d ata (PFKentai.txt)
QC d ata (PFQc.txt)
Scrip t CAL da ta(PFKenryo.txt)

Proto col
(scr_xx.scr)
mtesky Proto col file
Proto co l interp reta tio n an d p erformin g
Script interpreta tio n and p erfo rmin g
Mo tor contro l
I/O contro l
Position d ata
Variab les

ExtBoard

Motor controler Extension board(PMT,Printer,

Serial)

PFMain seeks available reagents from Item parameter file, Calibration data files and
QC data files (and configuration file=PFConfig.txt), then indicates for user and judges
if measurement is enable to be performed. PFMain also seeks these files at request
from user, and then indicates or prints information.
Whenever a measurement is begun, barcode reading protocol (bcr.scr) is performed,
and then the attributes of lanes are determined by seeking item parameter from the
barcode.
The next step is piercing; piercing protocol (scr_99.scr) is read and performed.
The protocol file and parameter file are determined (protocol file names are written in
the protocol parameter file) and performed by the attributes of lanes.
Motors are controlled by referring to the position data file during measurements.
Results of measurement (Count, Concentration) is processed in the order of from CAL
to QC, then written into each file. Finally, all measured data is written into sample
information file regardless of sample attribute.

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PATHFAST SERVICE MANUAL Chapter 6

6-5 Files and Folders

Type of file and location follows.

File name Contents Stored Comment

at
/
usr
sacs/
bin/
data/ Data updated by operation Can be target for Backup/Restore
PFCalPic.txt Master Calibration data CF
PFConfig.txt Setting data updated by user CF
PFConfig-E.txt Default Setting data (For CF
other countries)
PFConfig-J.txt Default Setting data (For CF
Japan)
PFKenryo.txt Calibration data CF
PFKentai.txt Sample data CF
PFQc.txt QC data CF
PFUser.txt User list CF
PFUserKomoku.txt Setting data according to CF
reagent parameter
PFUserSettei.txt Setting data updated by user CF
default/ Initial defualt data for flash CF
download/
fonts/ Font files
ini/ Setting data updated by
manufacturer
AxisSetting.txt Axis/Motor parameters Flash
PFConfig.ini CF information CF Software Version
Instrument.txt Instrument originated data Flash Serial-No, IP-Address, Optical
System, Teaching data,…
PFDefine.txt Designed data for teaching CF Position.txt is created by this
file and Instrument.txt.
PFKomoku.txt Reagent parameter CF At starting up, this file is over
written by PFKomoku0.txt(for
Japan) or PFKomoku1.txt(for Other
country ) according to setting
PFKomoku0.txt Reagent parameter (For Japan) CF
PFKomoku1.txt Reagent parameter (For other CF
countries)
PFPropara.txt Parameters for protocol Parameters for protocol to
execute protocols..
Position.txt Teaching data Flash
log/ Logging data
PFSystemLog.txt System error Log CF
PFUserLog.txt User Operation Log CF
Debug.log Sample Recognition Sensor CF
value and temperature Log
script/
upload/

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PATHFAST SERVICE MANUAL Chapter 6

6-6. Software Upgrade

The software upgrade may be needed in the following cases.

Program upgrade for problem solving or addition of functions
Addition of new assay items
Follow the procedure below to upgrade PATHFAST software.
Software upgrade is done by using PATHFAST CF card and the procedure below
is described assuming that the instrument power is on.

6-6-1．Changing Access Level

To upgrade software, the operator shall have the access level 4 or 5.

1. Change the user by selecting USER SWITCH on the [ADMIN] screen. The
following [USER SWITCH] screen is displayed.
USER SWITCH

2. Select the new user from the list shown by selecting the display box or ▽ .
Select “
Administrator”or “
System”as user ID.

3. Select OK to show the Full Keyboard to input the user ’

s password. Input
the password and select OK to go back to the [ASSAY/HOME] screen.
Password:
Access Level 4 Administrator: 4649
Access Level 5 System : 5963

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PATHFAST SERVICE MANUAL Chapter 6

6-6-2．Upgrading Software

Select ADMIN in sub-title area to show the [ADMIN] screen.

1. Select FILE UTILITY on the [ADMIN] screen. The following [FILE

UTILITY] screen is displayed

Note) The menu of DATA BACKUP is also displayed when you logged-in
as ”
System”in the following screen.

FILE UTILITY

2. Select UPGRADE to show the following [UPGRADE] screen. To start

software upgrade, select START button in navi area.
Note: Data backup cannot be canceled until it is completed.

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PATHFAST SERVICE MANUAL Chapter 6

UPGRADE 1

3. The following messages are displayed on the screen during data back up and
on completion of data back up.

UPGRADE 2 UPGRADE 3

4. When the above message is shown on the screen, shut the power down
following the messages shown on the screen.

5. While the instrument power is off, replace the currently installed CF

(Compact Flash) card with the new one.

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PATHFAST SERVICE MANUAL Chapter 6

< To Replace CF Card>

6. Open the printer cover at the lower front left of the instrument by pulling it
down toward you.

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PATHFAST SERVICE MANUAL Chapter 6

7. Push the lever on the left to open the printer paper cover as shown in the
picture below.

Caution : Take care not to be hurt by the paper cutter teeth.

8. Remove the printer paper. You will see the CF card set behind the printer
paper. The releasing rod for CF card is located on the right next to the card.
Push the releasing rod to push the card out. Remove the card. If needed you
may use tweezers.

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PATHFAST SERVICE MANUAL Chapter 6

9. With the label facing upward insert the new CF card.

Note: Push the card firmly enough to push out the releasing rod.

10. Replace the printer paper and close the printer cover. Turn the instrument
power on.
The following message will appear on the display asking if you want to
continue the software upgrade operation. Select OK to continue.
UPGRADE 4

11. After selecting OK , the following screens are shown.

UPGRADE 5 UPGRADE 6

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PATHFAST SERVICE MANUAL Chapter 6

12. After completion of shut down procedure, turn the instrument power on.
Confirm the software version is upgraded by selecting the ADMIN ,
SYSTEM and INSTRUMENT INFO. buttons consecutively.

6-7. Data Backup

The data stored in PATHFAST CF card can be backed up in a CF. Follow the
procedure below to backup data.
Prepare a CF with the same software as the instrument you work on, butwithout
data stored. It is used for storing the backed up data.

Note: Make sure that the customer allows you to make backup and to carry back
the backup CF for data or problem analysis.

6-7-1．Changing Access Level

To carry out data backup, the operator must have access level 5.

1. Change the user by selecting USER SWITCH on the [ADMIN] screen. The
following [USER SWITCH] screen is displayed.

USER SW ITCH

2. Select the new user from the list shown by selecting the display box or ▽ .
Select “
System”as user ID.

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PATHFAST SERVICE MANUAL Chapter 6

3. Select OK to show the Full Keyboard to input the user ’

s password. Input
the password and select OK to go back to the [ASSAY/HOME] screen.
Password:
Access Level 5 System : 5963

6-7-2．Data Backup Procedure

1. Select FILE UTILYTY on [ADMIN] screen. The following screen is

displayed.
FILE UTILYTY

2. Select DATA BACKUP to show the following screen. To start data backup,
select START button in Navi area.
Note: Data backup cannot be canceled until it is completed.
DATA BACKUP 1

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PATHFAST SERVICE MANUAL Chapter 6

3. The following messages are displayed on the screen during data back up and
on completion of data back up.
DATA BACKUP 2 DATA BACKUP 3

4. When the above message is shown on the screen, shut the power down
following the messages shown on the screen.

5. While the instrument power is off, replace the currently installed CF

(Compact Flash) card with backup CF.

< To Replace CF Card>

6. Open the printer cover at the lower front left of the instrument by pulling it
down toward you.

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PATHFAST SERVICE MANUAL Chapter 6

7. Push the lever on the left to open the printer paper cover as shown in the
picture below.

Caution : Take care not to be hurt by the paper cutter teeth.

8. Remove the printer paper. You will see the CF card set behind the printer
paper. The releasing rod for CF card is located on the right next to the card.
Push the releasing rod to push the card out. Remove the card. If needed you
may use tweezers.

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PATHFAST SERVICE MANUAL Chapter 6

9. With the label facing upward insert the backup CF card.

Note: Push the card firmly enough to push out the releasing rod.

10. Replace the printer paper and close the printer cover. Turn the instrument
power on.
The following message will appear on the display asking if you want to
continue the data backup operation. Select OK to continue.
DATA BACKUP 4

11. After selecting OK , the following screens are shown.

DATABACKUP 5 DATA BACKUP 6

12. After completion of shut down procedure, replace the backup CF with the
original CF. Then turn the instrument power on.

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PATHFAST SERVICE MANUAL Chapter 6

6-8．LCD Touch Panel Calibration

PATHFAST has the capability of adjusting touching points relative to the LCD
display. When you feel the touching points are displaced relative to the display,
you can calibrate the touch panel as follows.
Note: This calibration must be done with the product CF. Maintenance CF
does not have this function.
1. Turn the instrument power on and press [START] and [STOP] buttons
simultaneously during the following screen is displayed.

2. Release the buttons when you get the left upper screen in the following
drawing.

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PATHFAST SERVICE MANUAL Chapter 6

3. Touch the intersecting point (left lower) displayed on the screen in step 2.
The touched point becomes the calibrated point on the screen.
4. When stored the point in step 3, the next screen is shown. Touch the
intersecting point on the screen as well.
5. Repeat touching for the screens displayed consequently.
6. In the last screen touching the center point finishes the calibration and goes
back to the screen in step 1 and the system continues its initialization
operation.

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PATHFAST SERVICE MANUAL Chapter 7

Chapter 7 Maintenance CF and Teaching Operation

Maintenance CF has been developed to be used in production, performance tests,

maintenance, etc. and only English version is available.
Maintenance CF is used in place of PATHFAST Software CF. In the following section
functions of Maintenance CF are described, but the details are omitted for those
functions not needed for servicing in the field. Teaching procedures using
Maintenance CF are also described in this chapter.

Note 1: Maintenance CF has been developed to be used in production, testing or

maintenance of PATHFAST instrument. And it includes those functions
which shall not be used in the field, therefore do not use those functions
other than explained in this manual, such as teaching operations in this
chapter and functions used in periodic maintenance work described in
chapter 10.

Note 2: The specific version of Maintenance CF shall be used for corresponding

version of PATHFAST Software CF. Refer to the following table for
correspondence of software versions along with service manual
versions.

PATHFAST Maintenance CF Service Manual

Software Version Version Version
Ver. 2.0.0.*~2.0.1.* Ver. ｍcf1.0.2.0 Ver. 1.00
Ver. 2.0.2.* Ver. mcf 1.0.4.0 Ver. 2.00

Note 3: Service manual Ver. 1.00 only have 7 –2. Teaching in this manual with
some difference.

7 –1. Maintenance Menu

Maintenance CF is used in place of PATHFAST Software CF. While the

instrument power is off, replace PATHFAST Software CF with Maintenance
CF and turn the power on.
MAINTENANCE MENU screen in the next page is shown after initialization.
Abstract of each menu is described below.

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PATHFAST SERVICE MANUAL Chapter 7

MAINTENANCE MENU

1. AXIS OPERATION
From the screen shown by selecting this menu, manual operation of
each axis, execution of selected command or monitoring of sensor
functions can be performed.
This menu is used to check the teaching positions set by following
the procedures described in 7 –2 –3 Teaching Procedure for Each
Position. Also the function of AXIS OPERATION3 of this menu is
used instead of Manual Teaching in “
3. @TP.Y.PM and
@TP.PX.PMT(1).Center”in section 7 –2 –3.
Do not use other functions in this menu.

2. SENSOR MONITOR
From the screen shown by selecting this menu, you can manually
check the functionality of sensors of ORG, End Limit, PMT Shutter,
Door Lock, etc.
This menu is used for periodic maintenance work as well as SELF
DIAGNOSTIC menu. Refer to 10 –4 Sensor Check for detailed
operating procedure.

3. BCR OPERATION
From the screen shown by selecting this menu, you can perform read
test of reagent bar codes printed on the reagent cartridges by built-in

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PATHFAST SERVICE MANUAL Chapter 7

barcode reader. You can also check LED function, on or off. And
Hand-held Barcode Reader can be tested from this menu.
Refer to 7 –3 BCR OPERATION for detailed check procedures.

4. PMT OPERATION
From the screen shown by selecting this menu, you can check the
functionality of PMT manually. Refer to 10 –6 PMT Check for
detailed check procedures.

5. SAMPLE DETECTOR
From the screen shown by selecting this menu, you can manually
check the function or operation of Sample Recognition Sensor. Refer
to 10 –5 Sample Recognition Sensor Check for detailed check
procedure.

6. LAN SETTING
From the screen shown by selecting this menu, you can change IP
address of LAN port of PATHFAST. This menu is used in production
process.Do not use this menu for maintenance purpose.

7. LOG MONITOR
From the screen shown by selecting this menu, you can see the
logged information in Maintenance CF. Note that the displayed
information is not from PATHFAST Software CF that the customer
uses.
In ７－４ LOG MONITOR of this chapter, screens from both CF
cards are shown in parallel, logs and instrument information.

8. TEACHING
From the screen shown by selecting this menu, you can do teaching
operation for each position. Refer to 7 –2 –3 Teaching Procedure for
Each Position in this chapter for detailed teaching operation.

9. TEMP MONITOR
From the screen shown by selecting this menu, you can check the
temperatures of heater blocks and the chamber. Refer to 10 –7
Temperature Check for detailed operation.

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PATHFAST SERVICE MANUAL Chapter 7
10. HOST MONITOR
From the screen shown by selecting this menu, you can check the host
communication function of PATHFAST. Refer to 7 – 5 HOST
MONITOR in this chapter for detailed operation.

11. RUN PROTOCOL

From the screen shown by selecting this menu, you can run a protocol
from pre-defined ones. When servicing in the field, you only need to
run a protocol explained in 10 –8 Leakage Check in this manual. Do
not use other protocols.

12. SELF DIAGNOSTIC

From the screen shown by selecting this menu, you can run self
diagnostic test to check the function and operation of sensors, such as
ORG or End Limit sensors, automatically. Refer to 10 –4 Sensor
Check in this manual for detailed operation.

13. DISPENSE TEST

From the screen shown by selecting this menu, you can run a protocol
for dispense test. Further, you can input the volumes manually
measured to get mean and CV. When servicing in the field, you only
need to run a protocol explained in 10 –9 Dispense Check in this
manual. Do not use other protocols.

14. SETTING FILES

From the screen shown by selecting this menu, you can see the
contents of various setting files and rewrite H/L Ratio of LED counts
measured by PMT. When servicing in the field, you only need to
rewrite the H/L Ratio when you replaced parts related PMT.

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7 –2 Teaching
Teaching is an operation to adjust the relative positions among the Axis of the
PATHFAST instrument and is performed through the LCD screen using five types
of jigs.

7 –2 –1 Teaching Jigs
The following jigs are used for Teaching.

1. Jigs for Y-Axis Teaching

From the left, the jig for Tip position, Measurement well position and PMT
position (two pieces).

2. Jigs for Z-Axis Teaching

This is the jig for Z-Axis teaching. This jig is used by putting on the objects on
the stage and then contacting the nozzles onto the jig.

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PATHFAST SERVICE MANUAL Chapter 7

3. Jig for M-Axis teaching

This is the jig for M-Axis teaching. This jig is used by contacting the
instrument frame.

4. Positions to set the teaching jigs for Y-Axis and PX-Axis

Teaching jigs for Y-Axis or PX-Axis are set as in the following picture.

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PATHFAST SERVICE MANUAL Chapter 7

5．Jig for Waste Tip Box sensor teaching

This is the jig for Waste Tip Box teaching. This is used by setting instead of
plastic Waste Tip Box.

7 –2 –2 Basic operation of teaching

Turn the instrument power on with maintenance CF installed.

Teaching screen is shown by choosing “
TEACHING”button on the “
MAINTENANCE
MENU”
.

Here, the basic teaching operation on the teaching screen is described.

Details of each teaching operation will be described after this illustration.

Remove the covers before beginning work. Refer to the picture (Fig. 4-3-1) shown in
section 4-3 in Chapter 4.

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PATHFAST SERVICE MANUAL Chapter 7

The relationship between pulses and the traveling distance of each axis is shown in
the following list. When you manually operate the axis motors, pay careful attention
not to let the axis hit the target by changing the pulses for motor movement.

Axis
Pulses/mm mm/pulse Motion
Name
Y-Axis 20 0.0500 Front to back direction of the stage
Z-Axis 80 0.0125 Up and down direction of Z Unit
P-Axis 400 0.0025 Up and down direction of P Unit
M-Axis 80 0.0125 Front to back direction of M Unit
PX-Axis 20 0.0500 Left to right movement of PMT Shutter Unit

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PATHFAST SERVICE MANUAL Chapter 7

Screen Operation

To show teaching point list of target axis, select teaching point list box.

Choose teaching point of the target axis, then press OK button.

Axis label

Current Position

Step count setting box

Arrow buttons

Each axis can be moved to present teaching position by pressing Go

button.

After this, the position is trimmed manually.

The figure located on the right side of axis label indicates current position.

Step count setting box can be specified the step count of motion at a time

by pressing allow buttons. This box cycles the fixed numbers from 1 to

1000, 100, 10 then returns 1 by each pressing. Teaching position can be

trimmed by pressing arrow buttons. The pulse count to move at a time is

according to the setting of Step count setting box. Please follow detailed

teaching procedure for each axis.

Choose axis to write after trimming is finished. The teaching point that

shown in the teaching point list box of target axis will be the target.

By pressing “
Write Position”button, a confirmation message box is shown,

then press “
OK”button to write the new position.

During writing in progress, the title bar of the confirmation message box is

shown by white color, then the box will be closed when the writing is

completed. Writing takes around 15 minutes.

There is no sound of tapping buttons on the confirmation message box.

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About SAFE MODE

PMT Light Guide and the Stage (Y-Axis) may interfere each other depending on the
relative positions of PX-Axis and PZ-Axis. Therefore, the software inhibits their
movement as in the followings.

-. PX-Axis shall not move, when PZ-Axis is not at its home position.
-. The Stage (Y-Axis) shall not move, while PX-Axis is not its at home position and
PZ-Axis is not at its home position.

In teaching operation, fine tuning of the Stage (Y-Axis) and PX-Axis positions is made
while the PZ-Axis is in the lowered position, getting free from the above limitation.
For this purpose, select “
SAFE MODE”button on teaching screen to make it displayed
in normal, which means the “
SAFE MODE”is OFF. To make the “
SAFE MODE”ON,
select the button again and the button is displayed in reverse.

Pay careful attention when operating instrument with “

SAFE MODE”
OFF, not to
damage PMT Light Guide.

When getting out of the teaching screen, it automatically sets the ”

SAFE MODE”ON.

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7 –2 –3 Teaching procedure for each position

Note: Start teaching operation with showing TEACING screen by selecting

TEACHING in MAINENANCE MENU screen. And be sure that the Reagent
Cartridge Rack is not set on the instrument.

1. Teaching of @TP.Y.BlackWell
[Teaching Procedure]
1. Push “
ORG ALL”button to home all Axes.
2. Select “
@TP.Y.BlackWell”in the teaching point list box of Y, then press “
Go”
button.

3. Set jig in the hole for detection well of lane #6 position on the stage.

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4. Move Z-Axis down, then trim the Y-Axis as let nozzle enter the jig. At this point,
check if jig can be rotated smoothly by fingers and if the space around nozzle
from jig is the same.

5. After trimming is completed, select “

Y”at “
Write Axis”
, then press [Write
Position] button to write the new position.
6. Press “
ORG ALL”button.
7. Select RETURN to show MAINTENANCE MENU screen and select AXIS
OPERATION.
8. Select @Proc(10).Y.Center in the teaching point list box of Y, then press “
Go”
button to move Y-Axis. Then manually move Z-Axis down to the position where
the nozzle gets in the jig.
9. Check that the jig can be rotated smoothly by fingers to assure that Y-Axis is at
the teaching point of @TP.Y.BlackWell set above.
10. In case of periodic maintenance work, record the number of steps for Y-Axis.

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11. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

2. Teaching of @TP.Y.TipOn
[Teaching Procedure]
1. Push “
ORG ALL”button.
2. Select “
@TP.Y.TipOn”on the teaching points list box of Y, then press “
Go”button.

3. Set jig in the hole of lane #6 of the tip base on the stage.

4. Move Z-Axis down, then trim the Y-Axis as let nozzle enter the jig. At this point,
check if jig can be rotated smoothly by fingers and if the space around nozzle
from jig is the same.

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5. After trimming is completed, select “

Y”at “
Write Axis”
, then press [Write
Position] button to write the new position.
6. Press “
ORG ALL”button.
7. Select RETURN to show MAINTENANCE MENU screen and select AXIS
OPERATION.
8. Select @Tip(1).Y.Center in the teaching point list box of Y, then press “
Go”
button to move Y-Axis.
9. Select @Tip(1).Z.Standby in the teaching point list of Z, then select “
GO”button
to move Z-Axis.
10. Check that the jig can be rotated smoothly by fingers to assure that Y-Axis is at
the teaching point of @TP.Y.TipOn set above.

11. In case of periodic maintenance work, record the number of steps for Y-Axis.

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12. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

3. Teaching of @TP.Y.PMT and @TP.PX.PMT(1).Center

Teaching of @TP.Y.PMT and @TP.PX.PMT(1).Center positions are performed
simultaneously. It is recommended to do the work from the side or the back.
Note: There are two methods for this teaching operation, manual teaching and
automatic teaching prepared in Maintenance CF. Both procedures are
described separately.

[Manual Teaching Procedure]

1. Push “
ORG ALL”button
2. Select “
@TP.Y. PMT”on the teaching points list box of Y, then press “
Go”button.
3. Open the back cover, then set jig in the hole of lane #1 of black well on the stage.
4. Select “
@TP.PX.PMT(1).Center”on the teaching points list box of PX, then press
“
Go”button.

5. Select the “
SAFE MODE”button to make OFF (will be changed into normal
indication).
6. Move PZ-Axis down by 200 pulses, then trim the Y-Axis and PX-Axis to let light
guide enter the jig. At this point, check if jig can be rotated smoothly by fingers
and if the space around light guide from jig becomes the same.

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PATHFAST SERVICE MANUAL Chapter 7

7. After trimming is completed, select “

Y”at “
Write Axis”
, then press [Write
Position] button to write the new position.
8. Next, select “
PX”at “
Write Axis”
, then press [Write Position] button to write the
new position.
9. Select the “
SAFE MODE ”button to make ON (will be changed into reverse
indication).
10. Push “
ORG ALL”button
11. Again, select @TP.Y.PMT in the teaching points list box of Y, then press “
Go”
button.
12. Move the jig to the hole of lane #6 of black well on the stage.
13. Select @TP.PX.PMT(1).Center in the teaching point box of PX and press “
Go”
button. Then move PX-Axis by 2000 pulses, using right arrow.
14. Select the “
SAFE MODE”button to make OFF (will be changed into normal
indication).SAFE MODE」
15. Move PZ-Axis down by 200 pulses, then trim the Y-Axis and PX-Axis to let light
guide enter the jig. At this point, check if jig can be rotated smoothly by fingers
and if the space around light guide from jig is the same.
16. If the light guide does not fit on the jig or the space around the light guide is not
equal, repeat the adjustment from step 2 above checking the direction and the
degree of displacement.
17. When finished teaching operation, select RETURN to show MAINTENANCE
MENU screen and select AXIS OPERATION consequently.
18. Select @PMT.Y.Center in the teaching point list box of Y, then press “
Go”button
to move Y-Axis.
19. Select @PMT(1).PX.Center in the teaching point list of PX, then select “
Go”
button to move PX-Axis.

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PATHFAST SERVICE MANUAL Chapter 7

20. Move PZ-Axis down by 200 pulses and check if jig can be rotated smoothly by
fingers and if the space around light guide from jig is the same to confirm that
PMT is in the position set for @TP.Y.PMT and @TP.PX.PMT(1).Center.
21. Next, select @PMT(6).PX.Center in the teaching point list of PX, then select
“
Go”button to move Z-Axis to lane #6.
22. Move PZ-Axis down by 200 pulses and check if jig can be rotated smoothly by
fingers and if the space around light guide from jig is the same. Confirm that
PMT is in the position set for @TP.Y.PMT and @TP.PX.PMT(6).Center.
23. In case of periodic maintenance work, record the number of steps for both
positions.
24. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

[Automatic Teaching Procedure]

1. Select AXIS OPERATION in MAINTENANCE MENU screen and in the
displayed screen select NEXT button in Navi Area. Then select NEXT again in
the displayed screen to show AXIS OPERATION3 screen.

Note: The positions shown (numbers in parenthesis) in the screen are those
currently stored in PATHFAST as teaching points.

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PATHFAST SERVICE MANUAL Chapter 7

AXIS OPERATION3

2. Open the back cover, then set jigs in the holes of lane #1 and #6 of black well on
the stage.
3. Select “
Y”in Navi Area in AXIS OPERATION3 screen to start automatic
optimization protocol for @TP.Y.PMT position.

4. The above screens are shown during operation and after completion of the
operation.
5. Select WRITE POS button and OK button for confirmation message to store the
@TP.Y.PMT teaching position data.
Also the following printout is given on completion of the optimization operation.
Keep the printed tape as evidence record of implementation.

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PATHFAST SERVICE MANUAL Chapter 7

PMT fitting is checked for each position, moving PMT by one pulse
each time in the range of +/- 25 pulses from the stored @TP.Y.PMT
position. The result is judged by PZ sensor and is shown as “
X”for
fail or “
O”for successful fitting.

Printout Image
-------------------
PMT Y-POS OPTIMIZE
LANE1 LANE6
+25 X X

+18 X X
+17 X X
+16 X X
+15 X X
+14 X X
+13 X X
+12 X X
+11 X X
+10 X X
+9 X X
+8 X X
+7 X X
+6 O X
+5 O X
+4 O O
+3 O O
+2 O O
+1 O O
0 O O
-1 O O
-2 O O
-3 O O
-4 X O
-5 X O
-6 X X
-7 X X
-8 X X
-9 X X
-10 X X
-11 X X
-12 X X
-14 X X
-15 X X
-16 X X
-17 X X
-18 X X

-25 X X
--------------------

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6. Next select “
PX”in Navi Area in AXIS OPERATION3 screen to start automatic
optimization protocol for @TP.PX.PMT(1).Center & @TP.PX.PMT(6).Center
positions.

7. The above screens are shown during operation and after completion of the
operation.
8. Select WRITE POS button and OK button for confirmation message to store the
@TP.PX.PMT(1).Center and @TP.PX.PMT(6).Center teaching position data.
Also the following printout is given on completion of the optimization operation.
Keep the printed tape as evidence record of implementation.

Printout Image
-------------------------
PMT fitting is checked for PMT PX-POS OPTIMIZE
LANE1
each position, moving PMT by (-)
one pulse each time in the XXXXXXXXXXXXXXXXXXXXOOOOO
(0)
range of +/- 25 pulses from the O
stored @TP.Y.PMT position. (+)
OOOOOXXXXXXXXXXXXXXXXXXXX
The result is judged by PZ
sensor and is shown as “
X”
LANE6
for fail or “
0”for successful (-)
fitting. XXXXXXXXXXXXXXXXXXOOOOOOO
(0)
O
(+)
OOOXXXXXXXXXXXXXXXXXXXXXX
-------------------------

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9. Select RETURN in AXIS OPERATION3 screen to show AXIS OPERATION2

screen and again select RETURN to go back to AXIS OPERATION screen.
10. Select @PMT.Y.Center for Y position where to move and select “
Go”
.

11. Select @PMT(1).PX.Center for PX position and select “

Go”to move PMT to
lane#1.
12. Move PZ-Axis down to the jig and check if jig can be rotated smoothly by
fingers and if the space around light guide from jig is the same to confirm that
PMT is in the position set for @TP.Y.PMT and @TP.PX.PMT(1).Center.
13. Then select @PMT(6).PX.Center for PX position and select “
Go”to move PMT
to lane#6.
14. Move PZ-Axis down to the jig and check if jig can be rotated smoothly by
fingers and if the space around light guide from jig is the same to confirm that
PMT is in the position set for @TP.Y.PMT and @TP.PX.PMT(6).Center.
15. In case of periodic maintenance work, record the numbers of steps for both
positions.
16. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

4. Teaching of @TP.Z.Proc.BlackWell
[Teaching Procedure]
1. Push “
ORG ALL”button.
2. Select “
@TP.Y. BlackWell”on the teaching points list box of Y, then press “
Go”
button.
3. Set jig on the holes of the detection well on the stage.

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4. Select “
@TP.Z.Proc.BlackWell”on the teaching points list box of Z.
5. Move Z-Axis down until it contacts the jig. The contact can be judged by no
sticking when moving the jig on the stage.

6. After trimming is completed, select “

Z”at “
Write Axis”
, then press [Write
Position] button to write the new position.
7. Home Z-Axis.
8. Remove the jig from the stage.
9. Press “
ORG ALL”button.
10. Select RETURN to show MAINTENANCE MENU screen and then select AXIS
OPERATION.
11. Select @Proc(10).Y.Center for Y-Axis where to move and select “
Go”
.
12. Then @Proc(10).Z.Process for Z-Axis where to move and select “
Go”
.
13. Check if the jig can be rotated smoothly by fingers to confirm that the nozzle is in

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PATHFAST SERVICE MANUAL Chapter 7

the position set for @TP.Z.Proc.BlackWell.

14. In case of periodic maintenance work, record the number of steps for Z-Axis
positions.

15. When checkout is finished, select ORG to home the axes and then select RETURN
to show MAINTENANCE MENU screen.

5. Teaching of @TP.Z.Z.Tip(1).Standby
[Teaching Procedure]
1. Push “
ORG ALL”button.
2. Select “
@TP.Y.TipOn”on the teaching points list box of Y, then press “
Go”button.
3. Set jig on the tip base of the stage.

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PATHFAST SERVICE MANUAL Chapter 7

4. Select “
@TP.Z.Tip(1).Standby”on the teaching points list box of Z.
5. Move Z-Axis down until it contacts the jig. The contact can be judged by no
sticking by slipping the jig on the tip base.

6. After trimming is completed, select “

Z”at “
Write Axis”
, then press [Write
Position] button to write the new position.
7. Home Z-Axis.
8. Remove jig on the tip base of the stage.
9. Press “
ORG ALL”button.
10. Select RETURN to show MAINTENANCE MENU screen and then select AXIS
OPERATION.
11. Select @Tip(1).Y.Center for Y-Axis where to move and select “
Go”
.
12. Select @Tip(1).Z.Standby for Z-Axis where to move and select “
Go”
.

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PATHFAST SERVICE MANUAL Chapter 7

13. Check if the jig can be rotated smoothly by fingers to confirm that the nozzle is in
the position set for @TP.Z.Tip(1).Standby.
14. In case of periodic maintenance work, record the number of steps for Z-Axis
positions.
15. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

6. Teaching of @TP.PX.PMT.Home
[Teaching Procedure]
1. Push “
ORG ALL”button.
2. Open the cover on the back.
3. Select “
@TP.PX.PMT.Home”on the teaching points list box of PX, then press “
Go”
button.

4. Select the “
SAFE MODE”button to make OFF (will be changed into normal
indication).
5. Move PZ-Axis down, then trim the PX-Axis so that the PMT Light Guide fits on
the PMT Calibration Unit.
Note: When you see that the PMT light guide is shifted in Y direction at Home
position, loosen the Fixing Screw shown in the picture below and adjust
the position and then tighten the screw.

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PATHFAST SERVICE MANUAL Chapter 7

Fixing Screw

6. After trimming is completed, select “

PX”at “
Write Axis”
, then press [Write
Position] button to write the new position.
7. Select the “
SAFE MODE”button to make ON (will be changed into reverse
indication).
8. Push “
ORG ALL”button
9. Select RETURN to show MAINTENANCE MENU screen and then select AXIS
OPERATION.
10. Select @PMT.PX.Home for PX-Axis where to move and select “
Go”
.

11. Move PZ-Axis down and check if PMT Light Guide fits on PMT Calibration Unit.
You can check the fitting by moving PMT Light Guide manually up and down to
confirm that the PMT is in the position set for @TP.PX.PMT.Home.

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12. In case of periodic maintenance work, record the number of steps for PX-Axis
positions.
13. When checkout is finished, select ORG to home the axes and then select RETURN
to show MAINTENANCE MENU screen.

7. Teaching of @TP.M.BasePos
[Teaching Procedure]
1. Perform the shutdown procedure, then turn the instrument power off..
2. Open all covers of instrument.
3. Turn the instrument power on with Maintenance CF installed, and then go to the
TEACHING screen.
4. Press “
ORG ALL”button.
5. Select “
@TP.M.BasePos”on the teaching points list box of M.
6. Set jig on the right side flame as following picture.

7. Move M-Axis until the right edge of M-Axis contacts to the inside of “
L”shape of
the jig.

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PATHFAST SERVICE MANUAL Chapter 7

8. After trimming is completed, select “

M”at “
Write Axis”
, then press [Write
Position] button to write the new position.
9. Press “
ORG ALL”button.
10. Select RETURN to show MAINTENANCE MENU screen and then select AXIS
OPERATION.
11. Select @Axis.M.BasePos for M-Axis where to move and select “
Go”
.
12. Visually inspect that the M-Axis is in the position set for @TP.M.BasePos.
13. In case of periodic maintenance work, record the number of steps for M-Axis
positions.

14. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

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8. Teaching of @TipTrashSense.Y.Center
[Teaching Procedure]
1. Turn the instrument power on with Maintenance CF installed, and then go to the
TEACHING screen.
2. Select ORG ALL to home all axes.
3. Select “
@TipTrashSense.Y.Center “on the teaching points list box of Y.
4. Select “
Go”to move Y-Axis to the center of Waste Tip Box.
5. Set the jig for Waste Tip Box position teaching.

6. Move Y-Axis to the position where green indicator is activated and orange
indicator is deactivated. Record the pulses for Y-Axis.
7. Further move Y-Axis to the position where both green indicator and orange
indicator are activated. Record the pulses for Y-Axis.
8. Calculate the center position from the pulses obtained in steps 6 and 7, and then
move Y-Axis to the calculated position.

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PATHFAST SERVICE MANUAL Chapter 7

9. Check for the correct position and then select Y at “

Wri
teAxi
s”and press [Write
Position] button to write the new position.
10. Select ORG ALL to home all axes.
11. Select RETURN to show MAINTENANCE MENU screen and then select AXIS
OPERATION.
12. Select @TipTrashSense.Y.Center for Y-Axis where to move and select “
Go”
13. Visually inspect that the Y-Axis is in the position set for
@TipTrashSense.Y.Center
14. In case of periodic maintenance work, record the number of steps for Y-Axis
positions.

14. When checkout is finished, select ORG to home the axes and then select
RETURN to show MAINTENANCE MENU screen.

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7 –2 –4 Positions affected by each teaching

・Well 16
@TP.Y.BlackWell
・Well 15
Positions in the right drawing are
・Well 14
affected.
・Well 13
・Well 12
・Well 11

Detection well (Well 10)

・Well 9
・Well 8
・Well 7
・Well 6
・Well 5
・Well 4
・Well 3
・Well 2

・Well 1

・ Trash
@TP.Y.TipON
Two positions Sample Recognition positions
are affected Tip On positions
@TP.Y.PMT

Two positions are

affected.

Barcode reading positions

PMT Measuring positions

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@TP.Z.Proc.BlackWell
・Well 16

・Well 15
Positions in the right drawing are
・Well 14
affected.
・Well 13
・Well 12
・Well 11

・Well 10

・Well 9
・Well 8
・Well 7
・Well 6
・Well 5
・Well 4
・Well 3
・Well 2

・Well 1

Piercing positions

@TP.Z.Tip(1).Standby

Two positions Height of Sample Recognition

are affected Height of Tip On

@TP.M.BasePos
No other positions are affected.
@TP.PX.PMT(1).Center
Positions in the
right drawing
Barcode reading positions
are affected.
1～ 6 Lane

PMT Measuring Positions

1～ 6 Lane

@TP.PX.PMT.Home
No other positions are affected.
@TipTrashSense.Y.Center
No other positions are affected.

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7 –3. BCR OPERATION

Selecting BCR OPERATION in MAINTENANCE MENU screen shows the

following “ BCR OPERATION” screen.
From this screen you can check built-in barcode reader for LED function of On/Off
or reagent barcode reading. Also you can perform barcode reading test for
Hand-held Barcode Reader.

Note: Do not use functions other than described below.

BCR OPERATION

Lane No.

LED, ON/OFF

Lane Selection

Result Area

7 –3 –1. Built-in Barcode Reader

[Manual Operation]
1. Set Reagent Cartridge rack with Reagent Cartridges installed in the
instrument. Record barcode information of the Reagent Cartridge for each
lane.
2. Select ON for “
LED, ON/OFF”button to activate barcode reader LED.
3. Select lane no. for barcode reading test.
4. Select SINGLE button to read the Reagent Barcode for that lane.
5. The barcode information read is shown in Result Area and you can check if
the readout is the same as that you recorded in step 1.
6. Repeat steps 3 and 4 to check barcodes in other lanes.
7. When you finish the test, select OFF for “
LED, ON/OFF”button to deactivate
barcode reader LED.

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[Automatic Operation]
1. Set Reagent Cartridge rack with Reagent Cartridges installed in the
instrument. Record barcode information of the Reagent Cartridge for each
lane.
2. Select start lane number and end lane number respectively. The buttons
change from 1 through 5 for start lane and from 2 through 6 for end lane.
3. Select SCAN button to run automatic Barcode Read test.
4. The results are shown in Result Area and you can check if the barcodes read
are the same as those recorded in step 1.

7 –3 –2. Hand-held Barcode Reader

1. Selecting “
HandyBCR”button in navi area of BCR OPERATION screen shows
the following screen.
HANDY BCR OPERATION

2. When you read barcode of patient samples, the read barcode is shown in the
lower box. CLEAR button erases the shown barcode information.

3. Check if the read barcode information is the same as the printed barcode of the
sample.

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[Appendix: Setting of Hand-held Barcode Reader]

The types of barcodes readable by the Hand-held Barcode Reader are pre-set
at the factory before shipment. When you need to reset the settings to the
default by any reason, follow the procedure described below.
The setting can be done by reading the barcodes shown consequently.

Note: The following operation can be done either with PATHFAST Software
CF or Maintenance CF.

1. Reset to default settings.

2. Set RS232Communicatiion.

3. Set Beep Sound large.

4. Set Beep Interval as 2.

5. Set Beep Type as Monotone.

To Step 6

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6. Enable Ink Spread read function.

7. Enable Over Flow Control function.

8. Enable Inter Digit Control function.

9. Disable Puzzle Solver function.

10. Disable all barcode families.

Now all the barcode settings have

been cleared.
Hereafter set the following barcode
types. To step 11.

The Hand-held Barcode Reader can hold settings of four types of barcodes. The
following types of barcode are registered as defaults.
-. CODE 39
-. ITF
-. CODABAR（NW7）
-. CODE 128

Follow the steps below and read each barcode consequently.

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11. Register CODE 39.

12. Start setting of CODE 39. 13. 13. Set digits to be read.

14. Read 0 (zero). 15. Read 1 (one).

16. Read 2 (two). 17. Read 0 (zero).

18. Finish setting for CODE 39.

Now it can read CODE 39 barcodes

of 1 through 20 digits.

19. Next start registering ITF.

To step 20

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20. Read ITF barcode. 21. Read 0 (zero).

22. Read 1 (one). 23. Read 2 (two9.

24. Read 0 (zero). 25. Finish setting.

Now it can read ITF of 1 through

To step 26
20 digits.

26. Read CODABAR (NW7) barcode

to register.

27. Start setting for CODABAR (NW7). 28. Set digits to be read.

29. Read 0 (zero). 30. Read 1 (one).

To step 31

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31. Read 3 (three). 32. Read 4 (four).

33. Finish setting for CODABAR (NW7).

Now it can read CODABAR (NW7) of

1 through 34 digits.

To step 34

34. Lastly read the barcode on the right

to register CODE 128

35. Now all the setting procedure for 4 barcodes same as factory set is completed.

36. To assure the right settings, read the barcodes below for correct reading.

CODE 39

code39

1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

code39

1 2 3 4 5 6 7 8 9 0

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ITF

ITF ITF

1 2 3 4 5 6 7 8 9 0 1 2 34 5 6 7 8 9 0 1 23456 7890

CODABAR（NW7）

NW7

A 1 2 3 4 5 6 7 8 9 0 A

CODE 128

128 128

1 2 3 4 5 67 8 9 0 1 2 3 4 5 6 7 8 9 0 123 456 78 90

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7 –4. LOG MONITOR

Select LOG MONITOR in MAINTENANCE MENU screen to show the following

LOG MONITOR screen. From this screen you can see USER LOG, SYSTEM LOG
and Instrument Information stored in Maintenance CF.
PATHFAST Software CF that the customer has been using contains the
information of operation done by the customer and of errors happened in the past.
Such information can be displayed on the screen by selecting SYSTEM in ADMIN
screen with PATHFAST Software CF installed. In the followings, both screens are
shown side by side.
Maintenance CF PATHFAST Software CF
LOG MONITOR ADMIN/SYSTEM

7 –4 –1. USER LOG

1. Select USER LOG in both LOG MONITOR screen and ADMIN/SYSTEM
screen to show the screens below.
Maintenance CF PATHFAST Software CF
USER LOG USER LOG

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Note: The contents shown in both screens are the same kind, but not the
same information is shown because both CF have different logs.

In the central box, user logs are listed from the latest one. Each line consists
of date and code and the detail is shown in the lower box when selected.

2. The display format of the selected log is as follows:

Date/Time + Message Format
Refer to the Message Table below for each Message Format.

[Message Table]
Code# Message Format Contents
A001 System StartUp
A002 System ShutDown <UserID=%s> <UserID=%s>: Logged-in User Name
A003 AssayStart <UserID=%s> Ditto
A004 AssayEnd <UserID=%s> Ditto
A005 AssayStop <UserID=%s> Ditto
A006 AssayError <ErrCode=%s> <ErrCode=%s>:Error Code No. (refer to [Error Code
<UserID=%s> Table] below for details.
<UserID=%s>: Logged-in User Name
B001 User Setting Mode <Mode(ON/OFF)=%s>: ON or OFF
<Mode(ON/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
B003 User Add <New User=%s> <New User=%s>: New User Name
<UserID=%s> <UserID=%s>: Logged-in User Name
B004 User Edit <Edit User=%s> <Edit User=%s>: Edited User Name
<UserID=%s> <UserID=%s>: Logged-in User Name
B005 User Delete <Deleted <Deleted User=%s>: Deleted User Name
User=%s> <UserID=%s> <UserID=%s>: Logged-in User Name
C001 Time Out Setting <Time=%s> <Time=%s>: Time of setting
<UserID=%s> <UserID=%s>: Logged-in User Name
C002 Reagent Type Setting <Type(JPN/OTHER)=%s>: US/EU or JPN
<Type(JPN/OTHER)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
C003 Beep Setting <Mode(ON/OFF)=%s>: ON or OFF
<Mode(ON/OFF)=%s> <UserID=%s>: Logged-in User Name

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<UserID=%s>
C004 Print Setting <Mode(ON/OFF)=%s>: ON or OFF
<Mode(ON/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
C005 Reageon Setting <Reageon(USA/EUR/JPN)=%s>: USA, EU or JPN
<Reageon(USA/EUR/JPN)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
C006 Date Setting <previous <previous date=%s>: Date before change
date=%s> <changed date=%s> <changed date=%s>: Date after change
<UserID=%s> <UserID=%s>: Logged-in User Name
C007 Time Setting <previous <previous time=%s>: Time before change
time=%s> <changed time=%s> <changed time=%s>: Time after change
<UserID=%s> <UserID=%s>: Logged-in User Name
C008 Lang Setting <Lang(E/D/F/S/I/J)=%s>: E,F,G,S,I or J
<Lang(E/D/F/S/I/J)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
C009 Alarm Setting <Mode(ON/OFF)=%s>: ON or OFF
<Mode(ON/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
D001 RefInterval <Flag(ON/OFF)=%s>: ON or OFF
<Flag(ON/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
D002 RefInterval <ItemName=%s> <ItemName=%s> : Assay Item Name
<UserID=%s> <UserID=%s>: Logged-in User Name
D003 RefInterval <FlagType=%s> <FlagType=%s>:Quantitative of Qualitative
<UserID=%s> <UserID=%s>: Logged-in User Name
D004 RefInterval <UpperLv=%s> <UpperLv=%s>: Number of Upper Levels
<LowerLv=%s> <UserID=%s> <LowerLv=%s>: Number of Lower Levels
<UserID=%s>: Logged-in User Name
D005 CalReplicate <N=%s> <N=%s>: Replicates of Calibrator
<UserID=%s> <UserID=%s>: Logged-in User Name
D006 CAL ALERT <Flag(Day/Time)=%s>: Day or Time
<Flag(Day/Time)=%s> <Day=%s>: Number of Days
<Day=%s> <Time=%s> <Time=%s>: Number of Hours
<UserID=%s> <UserID=%s>: Logged-in User Name
D007 Hct% DEFAULT <Hct=%s> <Hct=%s>: Changed Hct% Value

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<UserID=%s> <UserID=%s>: Logged-in User Name

D008 Cal-F <ItemName=%s> <ItemName=%s> Assay Item Name
<UserID=%s> <UserID=%s>: Logged-in User Name
D009 Cal-F <SLOPE=%s> <SLOPE=%s> : Value for Slope
<UserID=%s> <UserID=%s>: Logged-in User Name
D010 Cal-F <INTERCEPT=%s> <INTERCEPT=%s>: Value for Intercept
<UserID=%s> <UserID=%s>: Logged-in User Name
D011 QC ALERT <Flag(Day/Time)=%s>: Day or Time
<Flag(Day/Time)=%s> <Day=%s>: Number of Days
<Day=%s> <Time=%s> <Time=%s>: Number of Hours
<UserID=%s> <UserID=%s>: Logged-in User Name
E001 QcLockOut <Flag(ON/OFF)=%s>: ON or OFF
<Flag(ON/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
E002 QcLockOut <ItemName=%s> <ItemName=%s>: Assay Item Name
<UserID=%s> <UserID=%s>: Logged-in User Name
E003 QcLockOut <Flag(Day/Time)=%s>: Day or Time
<Flag(Day/Time)=%s> <Day=%s>: Number of Days
<Day=%s> <Time=%s> <Time=%s>: Number of Hours
<UserID=%s> <UserID=%s>: Logged-in User Name
E004 QcLockOut <QC1(ON/OFF)=%s> <QC1(ON/OFF)=%s>: ON or OFF
<UpperLv=%s> <LowerLv=%s> <UpperLv=%s>: Value for Upper Limit
<UserID=%s> <LowerLv=%s>: Value for Lower Limit
<UserID=%s>: Logged-in User Name
E005 QcLockOut <QC2(ON/OFF)=%s> <QC2(ON/OFF)=%s>: ON or OFF
<UpperLv=%s> <LowerLv=%s> <UpperLv=%s>: Value for Upper Limit
<UserID=%s> <LowerLv=%s>: Value for Lower Limit
<UserID=%s>: Logged-in User Name
E006 QcLockOut <QC2(ON/OFF)=%s> <QC2(ON/OFF)=%s>: ON or OFF
<UpperLv=%s> <LowerLv=%s> <UpperLv=%s>: Value for Upper Limit
<UserID=%s> <LowerLv=%s>: Value for Lower Limit
<UserID=%s>: Logged-in User Name
F001 Lis Setting <Mode(ASTM/FIX/OFF)=%s>: ASTM, FIX or OFF
<Mode(ASTM/FIX/OFF)=%s> <UserID=%s>: Logged-in User Name
<UserID=%s>
F002 RS Setting <BaudRate=%s> <BaudRate=%s>: Value for Baud Rate

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 PATHFAST SERVICE MANUAL Chapter 7

<UserID=%s> <UserID=%s>: Logged-in User Name

F003 RS Setting <DataBit=%s> <DataBit=%s>: Value for Data Length
<UserID=%s> <UserID=%s>: Logged-in User Name
F004 RS Setting <Parity=%s> <Parity=%s>: Mode of Parity
<UserID=%s> <UserID=%s>: Logged-in User Name
F005 RS Setting <StopBit=%s> <StopBit=%s>: Number of Stop Bit
<UserID=%s> <UserID=%s>: Logged-in User Name
F006 RS Setting <BccChk=%s> <BccChk=%s>: BCC Check ON or OFF
<UserID=%s> <UserID=%s>: Logged-in User Name
F007 RS Setting <DispID=%s> <DispID=%s>: ID for Display among ID, PID
<UserID=%s> or Patient Name
<UserID=%s>: Logged-in User Name
G001 Quantitive <L=%s> <H=%s> <L=%s>: Limit for Flag L
<UserID=%s> Quantitive <L=%s> <H=%s>: Limit for Flag H
<H=%s> <ユーザー：%s> <UserID=%s>: Logged-in User Name
G002 Quantitive <2L=%s> <2H=%s> <2L=%s>: Limit for Flag 2L
<UserID=%s> <2H=%s>: Limit for Flag 2H
<UserID=%s>: Logged-in User Name
G003 Quantitive <3L=%s> <3H=%s> <3L=%s>: Limit for Flag 3L
<UserID=%s> <3H=%s>: Limit for Flag 3H
<UserID=%s>: Logged-in User Name
G004 Quantitive <4L=%s> <4H=%s> <4L=%s>: Limit for Flag 4L
<UserID=%s> <4H=%s>: Limit for Flag 4H
<UserID=%s>: Logged-in User Name
G005 Quantitive <5L=%s> <5H=%s> <5L=%s>: Limit for Flag 5L
<UserID=%s> <5H=%s>: Limit for Flag 5H
<UserID=%s>: Logged-in User Name
G006 Qualitative <L=%s> <H=%s> <L=%s>: Limit for Flag –
(NEG)
<UserID=%s> <H=%s>: Limit for Flag +(POS)
<UserID=%s>: Logged-in User Name
G007 Qualitative <L=%s> <2H=%s> <L=%s>: Limit for Flag –
(NEG)
<UserID=%s> <2H=%s>: Limit for Flag 2+
<UserID=%s>: Logged-in User Name
G008 Qualitative <L=%s> <3H=%s> <L=%s>: Limit for Flag –
(NEG)
<UserID=%s> <3H=%s>: Limit for Flag 3+
<UserID=%s>: Logged-in User Name

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G009 Qualitative <L=%s> <4H=%s> <L=%s>: Limit for Flag –

(NEG)
<UserID=%s> <4H=%s>: Limit for Flag 4+
<UserID=%s>: Logged-in User Name
G010 Qualitative <L=%s> <5H=%s> <L=%s>: Limit for Flag –
(NEG)
<UserID=%s> <5H=%s>: Limit for Flag 5+
<UserID=%s>: Logged-in User Name

[Error Code Table]

Error Description of Error
Code #
101 No valid calibration data available when assay started.

102 No valid QC data available when assay started.

103 System was in warming-up phase when assay started.

105 Temperature error detected for BLOCK L when assay started.

106 Temperature error detected for BLOCK L when assay started.

107 Temperature error detected for BLOCK L when assay started.

201 Front Cover was not closed when assay started.

202 Cover Lock error was detected when assay started.

203 No tip was set when assay started.

204 Initialization error was detected when assay started.

205 Waste Tip Box was not detected when assay started.

206 Used tip was detected when assay started.

207 Abnormal PMT dark count was measured when assay started.

208 No reagent cartridge was detected when assay started.

401 Calibration failed when CAL/QC/SAMPLE assay was run.

501 Reagent cartridge with unknown item was found when assay started.
502 Barcode read error occurred for some lane when assay started.

503 Incompatibility error occurred when assay started.

504 Reagent cartridge was not set for some lane when assay started.

5-5 Expired CAL/QC/REAGENT found in some lane when assay started.

506 No valid calibration data for some lane was found when assay started.

507 Luminous error was detected for some lane when assay started.
508 PMT Fitting error was detected for some lane when assay started.

509 Different type of reagent was found in some lane when assay started.

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510 No valid QC data for some lane was found when assay started.

511 Tip was not detected for some lane when assay started.
515 Reagent cartridge with incompatible protocol was found for some lane when assay started.

801 Front cover was not closed when assay started.

802 Cover Lock error was detected at other than assay start.

7 –4 –2. SYSYEM LOG

1. Select SYSTEM LOG in LOG MONITOR screen or in ADMIN/SYSTEM screen
to show the screens below.
Maintenance CF PATHFAST Software CF
SYSTEM LOG SYSTEM LOG

Note: The contents shown in both screens are the same kind, but not the
same information is shown because both CF have different logs.

In the central box, system logs are listed from the latest one. Each line
consists of date and code and the detail is shown in the lower box when
selected.

2. The display format of the selected log is as follows:

Date/Time + Message Format
And Message Format consists as follows:
Message Code + Message
These are same as those listed in Table 8 - 3 System Error Codes. Refer to
the table for details.

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7 –4 –3. INSTRUMENT INFO.

1. Select INSTRUMENT INFO in LOG MONITOR screen and in
ADMIN/SYSTEM screen to show the screens below.
Maintenance CF PATHFAST Software CF
INSTRUMENT INFO INSTRUMENT INFO

2. You see the followings in INSTRUMENT INFO screen with Maintenance CF.
SERIAL NUMBER Serial Number of the instrument
PROGRAM VERSION Software version number of the CF currently
installed
EXTBOARD VERSION Firmware version number of EXT Board
ASSAY COUNT Accumulated number of assay runs completed
with the instrument

3. You see the followings in INSTRUMENT INFO screen with PATHFAST

Software CF.
SERIAL NUMBER Serial Number of the instrument
PROGRAM VERSION Software version number of the CF currently
installed

7 –5. HOST MONITOR

Select HOST MONITOR in MAINTENANCE MENU screen to show the screen

below. In this screen you can check if the function of communicating with
external computer is normal.

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Note: When performing functional test above, put a loop back connector on the RS232
port.
HOST MONITOR

Data Input Box

Sent Data

Received Data

[Test Procedure]
1. Selecting “
Data Input Box”shows the DATA INPUT screen to input data to
be sent to host computer. You can input data to be sent through full keyboard
up to 20 bytes. Select OK to go back to HOST MONITOR screen.

DATA INPUT

2. Select SEND in HOST MONITOR screen. The sent data is shown in “

Sent
Data”box.
3. The data sent is received by PATHFAST through the loop back connector and

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shown in “
Received Data”box.
4. Check that data shown in step 2 and 3 above is the same.

7 –6. Editing H/L Ratio for PMT

Select SETTING FILES in MAINTENANCE MENU screen to show the screen

below. You can check or edit H/L Ratio for PMT Shutter Assembly starting from
this screen.

SETTING FILES

Note: Before editing H/L Ratio for PMT Shutter Assembly here, Measure
and record H/L Ratio following the procedure in 10 –6 PMT Check
in this manual.

[Editing Procedure]

1. Select EDIT H/L Ratio in SETTING FILES screen to show the next
screen.

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2. Selecting the box where the numeric number is shown, the following
screen is shown. Input new value and select OK to fix.

3. Select FLASH UPDATE to store the new value.

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PATHFAST SERVICE MANUAL Chapter 8

Chapter 8 Troubleshooting

In this chapter, remarks and error codes reported by PATHFAST, along with their meaning and measures to be taken, are described.

8-1 Assay Remarks (Sample, QC and CAL)

Remarks reported are usually related to assays and they consist of one or two digit alphanumeric characters.

Table 8 – 1 Remarks
Measures by User Measures To Be Taken (Field Service)
Remark Meanings Data Handling (ref)
Note the following at assay rerun:
・ Dispense the sample of 100µL without creating bubbles.
・ Check if the length of tip is not short.
If the problem persists, the Sample Recognition Sensor may suffer a
Add the remark to the result. breakdown which needs repair.
S No Sample found Asterisks are printed instead Rerun ・ Check A/D values of Sample Recognition Sensors, using
of data. maintenance CF. (See 10-5)
・ If any abnormality is found, replace the Sample Recognition
Sensor Unit. (See 4-7-4)
・ Check relevant cables and connectors.
・ If the problem persists, replace CPU board, etc. (See 5-5-4)
Add the remark to the result. NC remark occurs when calibration assay and sample/QC assay are
NT No Tip found Rerun
Asterisks are printed instead performed at the same time and the calibration results in failure.

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PATHFAST SERVICE MANUAL Chapter 8

of data ・ Check the error code for calibration failure and take necessary
measures, and then rerun calibration.
If the problem persists, repair is needed.
・ Use maintenance CF to perform PMT check. (See 10-6)
・ Check dispensing. (See 10-9) If any abnormality is found,
replace PMT Shutter Unit, P-Axis Unit, etc. (See 4-11-2, 4-8)
Normally, forgetting to set a tip causes an error stop at the check
just after start, and failure to mount the tip or tip dropped after
mounted adds NT remark to the result.
・ Check the status of discarded tips in the lane with NT remark
added.
No valid calibration Add the remark to the result. If tips are properly discarded in tip waste box, then the Sample
NC available on assay Asterisks are printed instead Rerun Recognition Sensor may have abnormality which needs repair.
completion. of data ・ Check A/D values of Sample Recognition Sensors, using
maintenance CF. (See 10-5)
・ If any abnormality is found, replace the Sample Recognition
Sensor Unit. (See 4-7-4)
・ Check relevant cables and connectors.
・ If the problem persists, replace CPU board, etc. (See 5-5-4)

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Rerun/Call service Fitting in detection well check just after assay start was normal, but
fitting had problem in metering at assay end. Check the following:
・ See if detection well of reagent cartridge is not deformed.
・ See if reagent cartridge rack is not deformed.
・ See if locking screw of reagent cartridge rack is not loosened to
stick out.
・ See if reagent cartridge is properly set on reagent cartridge
rack.
PMT fitting error. ・ See if reagent cartridge rack is properly set in the instrument.
Add the remark to the result.
PMT Light Guide ・ See if any foreign body does not exist in the part where reagent
BE Asterisks are printed instead
did not fit on the cartridge rack is set.
of data
detection well. If no abnormality is found in any of those, then teaching position
may be displaced which needs repair.
・ Use Maintenance CF to check teaching positioning. (See 3 of
7-2-3)
・ If not correct, re-teaching is needed. (See 3 of 7-2-3)
・ Also check Y-Axis, PMTX-Axis and their timing belts.

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Last count is ・ Use maintenance CF to perform PMT check. (See 10-6)

Add the remark to the result.
lower than ・ Check dispensing. (See 10-9) If any abnormality is found,
ED Asterisks are printed instead Rerun
predefined value replace PMT Shutter Unit, P-Axis Unit, etc. (See 4-11-2, 4-8)
of data
(50)
Temperature measured at assay start was OK, but temperature
measured during assay had an error.
・ Check temperature displayed on the screen.
・ If room temperature is 30°C or more, reduce the room
temperature with air conditioner.
Temperature of Heat Add the remark to all the
H1 Rerun If the problem persists, repair is needed.
Block L is too high result
・ Check the exhaust fan of reaction section for abnormality.
Replace it as needed (See 4-5-5)
・ Check heater unit, temperature controller1, etc and relevant
cables and connectors for abnormality. Replace them as needed.
(See 4-7-2, 5-3, 5-5-4)
Temperature measured at assay start was OK, but temperature
measured during assay had an error。
・ Check temperature displayed on the screen.
Temperature of Heat Add the remark to all the ・ If room temperature is 30°C or more, reduce the room
H2 Rerun
Block S is too high result temperature with air conditioner。
If the problem persists, repair is needed。
・ Check the exhaust fan of reaction section for abnormality.
Replace it as needed.

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・ Check heater unit, temperature controller2, etc and relevant

cables and connectors for abnormality. Replace them as
needed. (See 4-7-2, 5-3, 5-4)
Temperature measured at assay start was OK, but temperature
measured during assay had an error。
・ Check temperature displayed on the screen.
・ If room temperature is 30°C or more, reduce the room
temperature with air conditioner。
Temperature of Heat Add the remark to all the
H3 Rerun If the problem persists, repair is needed。
Block T is too high result
・ Check the exhaust fan of reaction section for abnormality.
Replace it as needed.
・ Check M-Axis Unit, temperature controller3, etc and relevant
cables and connectors for abnormality. Replace them as needed.
(See 4-9, 5-3, 5-5-4)
Temperature measured at assay start was OK, but temperature
measured during assay had an error。
・ Check temperature displayed on the screen.
・ If room temperature is 15°C or less, raise the room temperature
Temperature of Heat Add the remark to all the
L1 Rerun with air conditioner.
Block L is too low result
If the problem persists, repair is needed。
・ Check heater unit, temperature controller1, etc and relevant
cables and connectors for abnormality. Replace them as
needed (See 4-7-2, 5-3, 5-5-4)

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PATHFAST SERVICE MANUAL Chapter 8

Temperature measured at assay start was OK, but temperature

measured during assay had an error。
・ Check temperature displayed on the screen.
・ If room temperature is 15°C or less, raise the room
Temperature of Heat Add the remark to all the
L2 Rerun temperature with air conditioner. If the problem persists,
Block S is too low result
repair is needed。
・ Check heater unit, temperature controller2, etc and relevant
cables and connectors for abnormality. Replace them as
needed. (See 4-7-2, 5-3, 5-5-4)
Temperature measured at assay start was OK, but temperature
measured during assay had an error。
・ Check temperature displayed on the screen.
・ If room temperature is 15°C or less, raise the room
Temperature of Heat Add the remark to all the
L3 Rerun temperature with air conditioner.
Block T is too low result
If the problem persists, repair is needed。
・ Check M-axis unit, temperature controllers, etc and relevant
cables and connectors for abnormality. Replace them as
needed. (See 4-9, 5-3, 5-5-4)
・ Check A/D values of Sample Recognition Sensors, using
Error reported as
Add the remark to the result. maintenance CF. (See 10-5)
non-distinctive by
UK Asterisks are printed instead Rerun ・ If any abnormality is found, replace the Sample Recognition
Sample Recognition
of data Sensor Unit. (See 4-7-4)
Sensor
・ Check relevant cables and connectors.

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・ If the problem persists, replace CPU board, etc. (See 5-5-4)

・ Check for dirt, dust, foreign body, etc. within the Sample
Recognition Sensor. Clean/Remove them as needed.
If the problem persists, repair is needed。
LED light signal of
・ Check A/D values of Sample Recognition Sensors, using
Sample Recognition
ER Abort assay run Rerun/Call Service maintenance CF. (See 10-5)
Sensor is too low;
・ If any abnormality is found, replace the Sample Recognition
<600.
Sensor Unit. (See 4-7-4)
・ Check relevant cables and connectors.
・ If the problem persists, replace CPU board, etc. (See 5-5-4)
This remark is not an error remark but a “notification remark”.
Input Hct% value Print data as it is with If DF remark is added even if inputting Hct% value other than
DF was same as default remark added. Check Result default value, then it is determined as abnormal which needs repair.
value ・ Replace software related parts, such as CF card, CPU Board,
EXT Board, etc. (See 5-5-4)
QC may be deteriorated.
・ Check if the state of preservation had no problem.
・ If frozen article, check if you made the concentration uniform
Measured QC is out Print data with remark
OR Rerun by stirring after dissolution.
of control range added
・ If freeze-dry article, check if there was no problem in the
method of dissolving.
Insoluble substances such as fibrin clot may be contained.

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・ Presence of fibrin clot increases the value out of range.

Possible unusual value.
・ See 8-6 Cause and Countermeasures of Unusual Values.
If the problem persists in assay rerun even after checking the items
above, rerun calibration.
If it persists, repair is needed.
・ Use maintenance CF to perform PMT check. (See 10-6)
・ Check dispensing. (See 10-9)
・ Check temperature. (See 10-7)
・ If any abnormality is found, replace relevant parts such as PMT
Shutter Unit, P-Axis Unit, temperature controller, etc. (See
4-11-2, 4-8, 5-5-4)
Remark added for This remark is not an error remark but a “notification remark”
transmitted data In recalculation using changed Hct%, if RS remark is added for
which is recalculated transmitted data to the host, then it is determined as abnormal which
RS None None
using changed Hct% needs repair.
value to host ・ Replace software related parts, such as CF card, CPU board,
computer EXT board, etc. (See 5-5-4)
Abnormal ・ Use Maintenance CF to check teaching positioning.
luminescent count Add the remark to the result. ・ If not correct, re-teaching is needed. (See 3 of 7-2-3)
AE (count(1min)>1000 Asterisks are printed instead Rerun If the problem persists,
and ratio to of data ・ Use maintenance CF to perform PMT check. (See 10-6)
count(0) is less ・ Check dispensing. (See 10-9)

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than 1.2) ・ If any abnormality is found, replace the corresponding parts

such as PMT Shutter Unit, etc. (See 4-11-2)
Add the remark to the result. Replace software related parts, such as CF card, CPU Board,
Hct% calculation Recalculate with
HC Asterisks are printed instead EXT Board, etc. (See 5-5-4)
error changed Hct%
of data
Calculation error Replace software related parts, such as CF card, CPU Board,
Add the remark to the result.
other than HC EXT Board, etc. (See 5-5-4)
CI Asterisks are printed instead Rerun
above (Calculation
of data
impossible)

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8 - 2 Error Codes for Failed Calibration

The following errors are reported only when calibration assay fails. These errors are reported only when calibration, as a whole, determined as
failed, even though the assayed data for each calibrator seems to have no problems.
In these cases, error codes are added on the result print out and also seen on CAL CHECK screen. The error code consists of two numeric digits.
The table below lists the error codes along with their meanings and countermeasures.

Table 8-2 Error Codes for Failed Calibration

Measures by User
Code Meanings Data Handling Counter Measures To Be Taken
(ref)
CV% of CAL1 Calibration is failed and the Rerun Calibration ・ Sample other than CAL1 may have been assayed in either
counts is out of data is stored with error Lane1 or 2.
specified range. code. ・ Possible unusual value. (See 8-5)
If the problem persists
・ Possible poor suction due to deteriorated syringe. (See 8 of
01 10-2-2, 10-8, 10-9)
・ Use maintenance CF to perform PMT check. (See 10-6)
・ Check PMT Shutter Unit and M-Axis Unit for abnormality.
Replace/Adjust them as needed. (See 4-9, 4-11-2)
Also possibly affected by contamination, so clean tip holder,
stage surface, etc. (See 10-2-2)
CV% of CAL2 Calibration is failed and the ・ Sample other than CAL2 may have been assayed in either
02 Rerun Calibration
counts is out of data is stored with error Lane3 or 4.

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PATHFAST SERVICE MANUAL Chapter 8

specified range. code. ・ For items other than Myo, CAL2 may be poorly dissolved.
・ Check if no bubbles are produced when dispensing sample.
・ Possible unusual value. (See 8-6)
If the problem persists, perform the same mechanical check as
“01”.
・ Sample other than CAL1 may have been assayed in either
In Two Point Lane1 or 2.
Calibration, average Calibration is failed and the ・ Possible blank increase due to deteriorated reagent.
Rerun
03 of CAL1 counts is data is stored with error Æ Check storage condition.
Calibration
greater than code. ・ Possible unusual value. (See 8-6)
specified value. If the problem persists, perform the same mechanical check as
“01”.
In One Point Calibration is failed and the Rerun Calibration ・ Sample other than CAL2 may have been assayed in either
Calibration, average data is stored with error Lane3 or 4。
of CAL1 counts is code. ・ For items other than Myo, CAL2 may be poorly dissolved.。
smaller than ・ Also check if used sample is not the one long-preserved after
specified value. Or dissolution.
04 in Two Point ・ Possible poor reaction due to deteriorated reagent.
Calibration, average →Check storage condition.。
of CAL2 counts is ・CAL1 may be deteriorated.
smaller than the →Check storage condition.。
specified value. ・ Possible unusual value. (See 8-6)
If the problem persists, perform the same mechanical check as

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“01”.
In One Point Calibration is failed and the Rerun Calibration ・ For items other than Myo, the whole amount of dilution may
Calibration, average data is stored with error not have been used (dense) when dissolving. Æ Poor
of CAL1 counts is code. dissolution
larger than specified ・ Possible unusual value. (See 8-6)
05 value. Or in Two ・ Or sample may be contaminated.
Point Calibration, If the problem persists, perform the same mechanical check as
average of CAL2 “01”.
counts is larger than
the specified value.
Absolute value of Calibration is failed and the Rerun Calibration ・ Possible unusual value. (See 8-6)
“Cal F b” in Two data is stored with error ・ Sample other than CAL1 may have been used.
06 Point Calibration is code. If the problem persists, perform the same mechanical check as
larger than the “01”.
specified value.
Secondary count is Calibration is failed and the Rerun Calibration ・ Possible abnormal luminescence of CDP-star.
larger than the data is stored with error Æ Possible black well contamination.
specified value and code. Æ Possible tip contamination.
07
its ratio to primary If the problem persists, perform the same mechanical check as
count is smaller than “01”.
the specified value.
Calculation was Calibration is failed and the Rerun Calibration Perform the same mechanical check as “01”.
08
impossible. data is discarded.

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At least one remark Calibration is failed and the Rerun Calibration Take counter measures for the errors added.
09 is added for one the data is stored with error
calibrators. code.

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8–3 System Error Codes

System Error codes are detected and reported during power on/system initialization, or during instrument operation such as assay runs. They are
displayed as “System Error *****(MSG Code)”. System Errors are categorized by their origin and expressed by message codes. Messages in the
table are seen on the system log screen for each MSG code, English expression only.

Table 8 - 3 System Error Codes

Kind MSG Code Message Measures To Be Taken

Related with I0001 Failed to initialize CPU Turn the instrument power off and on to see if the problem persists.
System If it persists, replace CPU board, etc. (See 5-5-4)
Initialization I0002 Failed to initialize T-Net Board Turn the instrument power off and on to see if the problem persists.
If it persists, replace T-Net board, CPU Board, etc. (See 5-5-4)

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I0003 Failed to initialize Motor Controller Turn the instrument power off and on to see if the problem persists.
If it persists, check followings:
- On T-NET Board: LED4(Green) is ON and LED8(Red) is OFF.
- On Motor Driver Board: LEDs PWR(Red) is ON, RUN1 thru
3(Green) are ON, ERR1 thru 3(Red) are OFF.
If not, replace T-NET Board, Motor Driver Board, etc.(See 5-5-3,
5-5-4)
- Connector CN13 of T-NET Board and cables connected to Motor
Driver Boards.
If the problem persists, replace Motor Driver board, etc. (See 5-5-3,
5-5-4)
I0004 No response from EXT board Turn the instrument power off and on to see if the problem persists.
If it persists, check followings.
- Connector CN19 and cables on CPU Board.
- Connectors CN4, CN14 and DIP Switch settings on EXT Board.
- Firmware version of EXT Board.
If the problem persists, replace EXT board, etc. (See 5-5-3, 5-5-4)
I0011 Failed to PMT Power Use Maintenance CF card to check PMT Power On/Off and LED
counts. (See 10-4, 10-6)If any abnormality is found, replace PMT
Shutter Unit.(See 4-11-2)
I0012 The error occurred when the init.scr was Defect in software program is suspected. Replace CF card,
executed CPU Board, etc.(See 5-5-4)

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I0013 The error occurred when the init.scr was ・ Use maintenance CF to run self diagnostic test. (See 10-4)
executed ・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
I0014 The error occurred when the init.scr was Use Maintenance CF card to run self diagnostic test. (See 10-4) If
executed some error found, replace corresponding sensor or unit.
I0015 No response from Printer Check connectors and cables between Printer and EXT Board for
abnormality.(See 5-5-4) Replace them as needed. If the problem
persists, replace Printer I/F Board or Printer, etc. (See 5-5-4)
I0016 Status Error to Printer Check printer paper and Printer Paper Cover. Perform printing test
using Data Check menu. If the problem persists, check and replace
cables, connectors, Printer I/F Board, Printer, etc. as needed. (See
5-5-4)
I0017 Timeout to Printer Check printer paper and Printer Paper Cover. Perform printing test
using Data Check menu. If the problem persists, replace Printer I/F
Board, Printer, etc. (See 5-5-4)
I0020 Unexpected error Temperature of Heat Block L/S/T did not reach to the specified
range after forty minutes elapsed from warming up completion.
Check temperature displayed on the screen for abnormality.
Turn the instrument power off and on to see if the problem persists.
If it persists, replace Heater unit.(See 4-7-2)
Related with F0050 File could not be opened Turn the instrument power off and on to see if the problem persists.
File Handling If it persists, replace CF card or CPU board, etc. (See 5-5-4)

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F0051 File could not be read Turn the instrument power off and on to see if the problem persists.
If it persists, replace CF card or CPU board, etc. (See 5-5-4)
F0052 File could not be written Turn the instrument power off and on to see if the problem persists.
If it persists, replace CF card or CPU board, etc. (See 5-5-4)
F0053 File does not exist Turn the instrument power off and on to see if the problem persists.
If it persists, replace CF card or CPU board, etc. (See 5-5-4)
Related with M0100 Axis Motor was in operation, command ・ Turn the instrument power off and on to see if the problem
Motors failed to be executed persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If it persists, check and replace cables, connectors between
T-NET board and Motor Driver board, or Motor Driver Board,
etc.

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M0101 Axis Motor was stopped in emergency Occurs if either motor unit does not normally operate during
initialization at assay start. Also occurs if front cover is opened
during assay.
・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
Door switch, Motor Driver Board, PSR Board, etc.
M0102 Axis Motor could not be operated because ・ Turn the instrument power off and on to see if the problem
of activated interlock persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
Bottom sensor of PZ-Axis.

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M0103 Axis Motor was stopped because of ・ Turn the instrument power off and on to see if the problem
activated +limit sensor persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
M0104 Axis Motor was stopped because of ・ Turn the instrument power off and on to see if the problem
activated -limit sensor persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
M0105 Axis Motor was moved to a position out of ・ Turn the instrument power off and on to see if the problem
range persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace sensors or unit.
If the problem disappears, protocol in CF may be damaged.

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M0106 Axis Motor timed out for its movement ・ Check for any obstruction in moving range of the axis.
・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
・ If cables and connectors are normal, replace the corresponding
Motor Driver Board, connector or cable.(See 5-5-4)
M0107 Nozzle hit obstruction during moving ・ Check for any obstruction on the stage.
downward ・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, use Maintenance CF and check
sensor function by Self Diagnostics.(See 10-4)
・ If any abnormality is found, check cables and connectors
related to the corresponding sensor or unit.
P-axis Unit, etc.
Related with D0150 Aspiration was attempted more than tip Problem with script command in protocol is suspected.
Tips volume ・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, replace CF card.

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D0151 Dispense was attempted more than aspirated Problem with script command in protocol is suspected.
volume ・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, replace CF card.
Related with P0200 Failed to open PMT Shutter ・ Turn the instrument power off and on to see if the problem
PMT persists.
・ If the problem persists, use Maintenance CF to check PMT
Shutter’s open/close function. (See 10-4)
・ If any abnormality is found, replace PMT Shutter Unit.(See
4-11-2)
P0201 Failed to close PMT Shutter ・ Turn the instrument power off and on to see if the problem
persists.
・ If the problem persists, use Maintenance CF to check PMT
Shutter’s open/close function. (See 10-4)
・ If any abnormality is found, replace PMT Shutter Unit.(See
4-11-2)
P0202 No PMT count data Use Maintenance CF card and check the PMT Shutter function and
LED counts.(See 4-11-2) If problem persists, replace PMT Shutter
Unit. Also, problem with script command in protocol is suspected.
Replace CF card.
P0203 Detected PMT error Use Maintenance CF card and check the PMT Shutter function and
LED counts. If problem persists, replace PMT Shutter Unit. (See
4-11-2)

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Related with T0250 Error detected during setting temperature for Communication Error between CPU Board and Temperature
Temperature Temperature Controller Controller is detected. Use Maintenance CF card and check the
Controller temperature displayed. If the problem persists, check cables or
replace Temperature Controller, etc.(See 5-5-4)
T0251 Error detected during getting temperature Communication Error between CPU Board and Temperature
from Temperature Controller Controller is detected. Use Maintenance CF card and check the
temperature displayed. If the problem persists, check cables or
replace Temperature Controller, etc. (See 5-5-4)
Related with H0350 Communication cable is not connected Currently not used.
Host Computer H0351 No response from Host Computer Host Communication can not be established. Check communication
cable, or check if the host is ready to communicate.
Related with S0400 Line %s: Incorrect parameter Errors categorized in this kind are closely related to assay protocols.
protocol/ Normally these are seen with protocols under development. Turn
commands the instrument power off and on to see if the problem persists. If
the problem persists, replace CF card. (%s means line number in
the protocol.)
S0401 Line %s: Not defined script command Ditto.
S0402 Line %s: Interrupted by user during Ditto.
execution
S0403 Line %s: Not defined VP table selected Ditto.
S0404 Line %s: Too many characters Ditto.
S0405 Line %s: Syntax error Ditto.
S0406 Line %s: Not defined variable was referred Ditto.

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S0407 Line %s: Label not found Ditto.

S0408 Line %s: Too many functions used Ditto.
S0409 Line %s: Too many variables used Ditto.
S0410 Line %s: Duplicate variable definition is Ditto.
made in the same scope
S0411 Line %s: Incorrect argument Ditto.
S0412 Line %s: Main( ) does not exist in the Ditto.
protocol

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8-4 Messages shown in popup screen (Caution, Alert, Indication, Error, etc.)

PATHFAST displays messages in a popup screen to inform the operator of cautions, alert, indication, error, etc. It is easy for operator to
understand these messages and to take further action by the operator. Some of these are rarely seen in normal operation.
These messages are categorized and listed in the following sections.

8-4-1 Major Messages Shown during System Initialization

Table 8−4 MSG: During System Initialization

No. Message Shown on the screen Meanings and Action To Be Taken
1 FRONT COVER NOT LOCKED. Front cover cannot be locked normally during system initialization. Front cover may
MAKE SURE THE COVER IS not have been properly closed. Close the cover completely and retry.
COMPLETELY CLOSED. If this message is given even for the correct operation, possible causes are: improperly
THEN, PRESS [RETRY]. positioned door lock pin, poorly operating door lock pin, displaced hole into which
door lock pin goes due to displaced exterior cover. (See 4-10)
2 CHECK PRINTER. During the system initialization after power on, it checks if printer paper is set or
paper holder cover is locked. This message asks you to check both.
3 USER OR PASSWORD IS NOT In Log-in operation, input data of user name or password is incorrect. This message
CORRECT. CAPITAL AND SMALL asks you to input again. Be aware that capital and small letters are distinguished.
LETTERS ARE DISTINGUISHED IN
THE PASSWORD.
4 SET TIP WASTE BOX. During system initialization check, it could not find tip waste box.
・ Set the tip waste box and close the front cover, then retry.

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・ If this message is given even when the tip waste box is set, check if the tip waste
box is not largely deformed.
・ If no abnormality is found, detected position of tip waste box may be incorrect, or
waste tip sensor itself may have problem which needs repair.
・ Perform teaching of tip waste box. If the problem persists, replace waste tip
sensor. (See 4-7-3)
8-4–2 Major Messages Shown after Assay Run Started

Table 8 – 5 MSG: After Assay Run Started

No. Message Shown on the screen Meanings and Action To Be Taken
1 DISCARD TIPS IN TIP WASTE The system performs various checks before starting actual assay operation. Used tips
BOX. from previous run were found in tip waste box.
・ Discard used tips and close the front cover, then retry.
・ If this message is given even when no used tips exist, then check if tip waste box
is not largely deformed or not set in a slanting position.
・ If no abnormality is found, detected position of waste tip may be incorrect, or
waste tip sensor itself may have problem which needs repair.
・ Perform teaching of tip waste box. If the problem persists, replace waste tip
sensor. (See 4-7-3)
2 NO TIP DETECTED. Check after assay start found not a single tip in tip holder.
SET TIPS, CLOSE FRONT COVER ・ Set a tip and close the front cover, then retry.
AND PRESS [RETRY]. ・ If this message is given even when tip is set, the Sample Recognition Sensor may
suffer a breakdown which needs repair.

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PATHFAST SERVICE MANUAL Chapter 8

・ See Countermeasures of NT Remark.

3 FRONT COVER OPEN. The system performs various checks before starting actual assay operation. It found
CLOSE THE COVER AND PRESS Front Cover is open.
[RETRY]. ・ Close the cover and restart the run.
・ If the message is given even when properly closed, cover close detection sensor
may be displaced, or sensor itself may have problem which needs repair.
4 SET TIP WASTE BOX. Tip waste box is not set.
・ Set the tip waste box and close the front cover, then retry.。
・ If this message is given even when the tip waste box is set, check if the tip waste
box is not largely deformed.
・ If no abnormality is found, detected position of tip waste box may be incorrect, or
waste tip sensor itself may have problem which needs repair.。
・ Perform teaching of tip waste box. If the problem persists, replace waste tip
sensor. (See 4-7-3)
5 FRONT COVER NOT LOCKED. Front cover cannot be locked normally during system initialization.
MAKE SURE THE COVER IS ・ Front cover may not have been properly closed. Close the cover completely and
COMPLETELY CLOSED. retry.
THEN, PRESS [RETRY]. ・ If this message is given even for the correct operation, possible causes are:
improperly positioned door lock pin, poorly operating door lock pin, displaced
hole into which door lock pin goes due to displaced exterior cover. (See 4-10)
6 FOLLOWING ERROR(S) DETECTED. The following messages are given after the system read barcodes on the reagent
TAKE APPROPRIATE ACTION. cartridges. They are reported with the lane number.

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UNKNOWN ITEM Reagent cartridge with lot number which has not been registered was found. Perform
MC Entry operation to register the master calibration data and run CAL Assay. When
QC Lock Out is On, QC assay is also needed. Or delete the lane information to run
assay for other samples.

READ ERROR Failed to read reagent barcode. Retry or replace the reagent cartridge.

INCOMPATIBILITY Different reagent cartridge from the designated one by host computer is set, or
different reagent cartridge from that placed before retry is set in the lane. Replace
with correct reagent cartridge.

NO CARTRIDGE Reagent cartridge designated by the system based on information from host computer
is not set in the lane. Place correct cartridge referring to [SAMPLE INFO] screen.

REAGENT EXPIRED Reagent cartridge set in the lane is expired. Replace with correct cartridge.

NO CAL DATA Valid calibration data for the reagent cartridge set in the lane does not exist. Run CAL
assay or delete lane information to run assay for other samples.

NO QC DATA This message is only seen when QC Lock Out is On. Valid QC data for the reagent
cartridge set in the lane does not exist. Run QC assay or delete lane information to run
assay for other samples.

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NO TIP Tip was not found in the lane where reagent cartridge is set. Set new tip and restart the
assay run.

NOT USED No reagent cartridge was found, which means the lane is not used.

LUMINOUS ERROR Abnormal luminescence was detected in the detection well in the lane. Replace the
reagent cartridge with new one to restart the assay run.

PMT POS. ERROR Poor fitting occurred in metering well check just after assay start.
Check the following:
・ See if detection well of reagent cartridge is not deformed.
・ See if reagent cartridge rack is not deformed.
・ See if locking screw of reagent cartridge rack is not loosened to stick out.
・ See if reagent cartridge is properly set on reagent cartridge rack.
・ See if reagent cartridge rack is properly set in the instrument.
・ See if any foreign body does not exist in the part where reagent cartridge rack is
set.
If no abnormality is found in any of those, then teaching position may be displaced
which needs repair.
・ Use Maintenance CF to check teaching positioning. (See 3 of 7-2-3)
・ If not correct, re-teaching is needed. (See 3 of 7-2-3)
Also check Y-Axis, PMTX-Axis and their timing belts.

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7 PROTOCOL INCOMPATIBILITY Protocol for running assay with reagent cartridges set is not common for all
cartridges. Check if correct reagent cartridges are set.
8 PROTOCOL ERROR Error found in the protocol. CF card may be damaged.
9 CHAMBER TEMPERATURE OUT OF Chamber temperature measured at assay start is out of the specified range. Restart the
LIMITS. assay run after leaving the instrument with Front Cover closed for a while.
10 BLOCK L TEMPERATURE OUT OF Block L temperature measured at assay start is out of the specified range. Restart the
LIMITS. assay run after leaving the instrument with Front Cover closed for a while.
11 BLOCK S TEMPERATURE OUT OF Block S temperature measured at assay start is out of the specified range. Restart the
LIMITS. assay run after leaving the instrument with Front Cover closed for a while.
12 BLOCK T TEMPERATURE OUT OF Block T temperature measured at assay start is out of the specified range. Restart the
LIMITS. assay run after leaving the instrument with Front Cover closed for a while.
13 NO VALID CAL DATA. The message shows no valid CAL data exists to run patient or QC samples. Abort the
assay run and run CAL assay first.
14 NO VALID QC DATA. This message is only seen when QC Lock Out is On. Valid QC data for the reagent
cartridges set does not exist for running assay of patient samples. Abort the assay run
and run QC assay first.
15 NO CARTRIDGE DETECTED. No reagent cartridge is set on the reagent cartridge rack.
16 PRINTER PAPER EMPTY. Printer paper is used out. Replace with new one.
PLACE NEW ONE AND PRESS
[RETRY].
17 ABNORMAL DARK CURRENT. Measured value of dark count at PMT home position is out of specification.
・ Remove any abnormal material at home position.
・ Use Maintenance CF card to check correct positioning of PMT at the home

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position.
・ If not, re-teaching is needed.
If the problem persists, replace PMT Shutter Unit.(ref.to 4-7-2)
18 CLOSE PRINTER COVER. Printer Paper Cover is open. Closer the cover completely.

8-4–3 Major Messages Shown during MC Entry Operation

Table 8 – 6 MSG: MC Entry Operation

No. Message Shown on the screen Meanings and Action To Be Taken
1 ERROR(S) OCCURRED AT THE MC Entry operation was attempted for non-registered assay item in PATHFAST. You
TIME OF CAPTURING BARCODE may need to upgrade the software version of CF card.
DATA IN "MC ENTRY CARD"
NO RELEVANT ITEM FOUND.
2 ERROR(S) OCCURRED AT THE Reagent lot in MC Entry Card is expired, or the wrong MC Entry card was used.
TIME OF CAPTURING BARCODE
DATA IN "MC ENTRY CARD"
REAGENT EXPIRED.
3 ERROR(S) OCCURRED AT THE The digits of the barcode read by the Handheld Barcode Reader do not match with
TIME OF CAPTURING BARCODE regular one. Be sure the light from Barcode Reader hits the whole printed barcode on
DATA IN "MC ENTRY CARD" the MC Entry Card.
NUMBER OF DIGITS MISMATCHED.

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PATHFAST SERVICE MANUAL Chapter 8

8-4–4 Major Messages Shown in Host Communication

Table 8 – 7 MSG: Host Communication

No. Message Shown on the screen Meanings and Action To Be Taken
1 NO ORDERS RECEIVED. No assay information was received from the host computer, when queried using SID
CHECK SID. read by Handheld Barcode Reader or input manually. The possibility of the message
is wrong SID or the order information for the sample has note been registered in the
host computer.
2 EXCEED CAPABLE TEST NUMBERS. This message tells you the excessive number of assays ordered than that of
ASSAY THIS SAMPLE IN NEXT PATHFAST is affordable with the cartridge rack. You need to make query operation
BATCH. for the last sample in the next batch run.
SID: The message is also printed as a reminder.

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PATHFAST SERVICE MANUAL Chapter 8

8-5 Other Trouble

Trouble Cause Measures To Be Taken

Poor reproducibility of QC. ・ QC may be deteriorated. ・ Check if the state of preservation had no problem.
・ If frozen article, check if you made the
concentration uniform by stirring after dissolution.

・ Check the presence of insoluble substances ・ Presence of fibrin clot increases the value out of
such as fibrin clot. range.

・ Possible poor suction of sample or sample ・ Dispense the correct amount of sample.
dilution. ・ Check if no bubbles are present at the bottom of
sample well.

・ Possible unusual value. ・ See 8-6.

Sample cannot be assayed despite the ・ Check if calibration is not overdue. ・ Check calibration due.
reagent is within the expiration date. ・ Check calibration curve information.
・ Check QC lockout.
・ Faulty instrument Æ Possible bug ・ Maintenance of the instrument

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PATHFAST SERVICE MANUAL Chapter 8

8-6 Cause and Countermeasures of Discontinuous Values

Cause Check Point Measures To Be Taken

Poor suction of sample dilution Check if no bubbles are present at the bottom of If bubbles are present, tap it lightly on the table to
well. remove the bubbles.
Scattered magnetic particles inside the well. Make a visual observation of magnetic particle If the scatter found, possible causes are: poor storage
well. condition and poor transportation condition.
Contaminated cartridge was used. ・ Check if dropped cartridge was not used. Use a new cartridge.
・ Check if you never touched the detection
well.
・ Check if you never dispensed the sample
into the detection well.
Contaminated by ALP, etc of instrument ・ Difficult to determine by visual observation. Clean the piercer.
piercer.
Contamination of the tip end ・ Check if tip dropped onto the floor is not Use a new tip.
used.
・ Check if you never touched the tip end.
Bent tip ・ Make a visual check. If bent tips are present, possible causes are: poor
・ Tip bent by 1mm or less conforms to the storage and poor transportation. Never use possible
standard. such tips.

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PATHFAST SERVICE MANUAL Chapter 9

Chapter 9 Spare Parts

In this chapter, replaceable parts are listed below.

Spare Parts List

Part Number Description Note
512-05-021-M00 P-Axis Unit Assembled/Tested
512-05-022-M00 M-Axis Unit Assembled/Tested
512-05-023-M00 Syringe Unit Assembled/Tested
512-05-031-M00 Heater Unit Assembled/Tested
512-05-033-M00 PMT Correction Unit Assembled/Tested
512-05-072-M00 Door Lock Unit Assembled/Tested
512-05-11-M00 PMT Shutter Unit Assembled/Tested
512-07-510-k5680 Barcode Reader Unit Assembled/Tested
512-05-020-K0020 Z-Axis Motor Timing Belt
512-05-021-K0020 P-Axis Motor Timing Belt
512-05-030-K0010 Y-Axis Motor Timing Belt
512-01-112-K0021 PX-Axis Motor Timing Belt
512-02-020-M0270 Z-Axis Motor w/ cable & connector
512-01-030-K0040 Stepping Motor w/ cable & connector
512-04-111-K0200 Stepping Motor w/ cable & connector
512-04-030-M0410 Air Filter
512-04-030-K0190 Waste Tip Sensor w/ cable & connector
512-01-020-K0130 Photo Sensor w/ cable & connector
512-02-020-K0070 Photo Sensor w/ cable & connector
512-02-020-K0080 Photo Sensor w/ cable & connector
512-02-111-K0160 Photo Sensor w/ cable & connector
512-02-070-K0010 Printer
512-02-070-K0020 Printer I/F Board
094-02-070-K0220 Interlock Switch
512-02-070-K0030 LCD Display with Touch Panel
512-02-070-K0040 Inverter Board
AHE80010D T-NET Board
AHE88101C CPU Board
AHS88101B EXT Board

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PATHFAST SERVICE MANUAL Chapter 9

AHE80007D CF I/F Board

AHE80008D LCD I/F Board
512-04-520-E1000 PSR Board
5.10E+12 TEMP PCB Assy
512-05-510-K0040 Thermostat w/ cable & connector
512-04-510-K5310 Motor Driver Board
512-04-510-K5260 Temperature Controller
512-04-510-K5020 DC5V Power Supply
512-04-510-K5030 DC24V Power Supply
512-04-510-K5100 FAN w/ cable & connector
512-04-510-K5110 FAN w/ cable & connector
512-02-070-M0020 Front Cover
512-02-070-M0091 Operation Panel
512-02-070-M0131 Lower Front Cover
512-02-070-M0141 Side Cover L
512-02-070-M0151 Side Cover, R
512-07-038-M00 Sample Recognition Sensor Unit Assembled/Tested
PF-T-0001 Maintenance Tool Kit
51207-901M001 Jig for Tip position
51207-901M002 Jig for Measurement well position
51207-901M0032 Jig for PMT position II
51207-901M004 Jig for Z-Axis teaching
51207-901M005 Jig for M-Axis teaching
51207-901M006 Jig for adjusting Reagent Cartridge
Rack
51207-901M007 Jig for Dispense Check
51207-901M008 Spacer for Air Filter Exchange
899-302M001A Jig for Tip Waste Box position

Note) Maintenance Tool Kit will contain jigs needed for maintenance work.
Boards are all tested.

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PATHFAST SERVICE MANUAL Chapter 10

Chapter 10 Periodic Maintenance

It is recommended that periodic maintenance be completed once every 6 months.

Required tools and disposables and the maintenance procedures are explained
below.

１０−１ Tools and Disposables Needed

The tools and disposables listed below are needed to perform maintenance work.

<Tools>
- Maintenance CF card
- Phillips screw driver (+), size #1 and #2
- Ball point hex wrench set (long), 1.5mm, 2mm, 2.5mm, 3mm and 4mm
- Repetitive Pipette
- Pipette, Gilson Pipetteman P-100
- Pen-type flash light
- Mirror
- Jig for adjusting Reagent Cartridge Rack
- Jig for Dispense Check
- Teaching Tools

<Disposables>
- Ethanol
- Soft, gentle, absorbent low-lint wipes for light-duty tasks. or
- Soft, non- abrasive low-lint wipes that are considered safe for use on most
delicate surfaces.
- Oil pen
- Metal Clean
- Cotton swabs
- Tooth Brush
- Disposable tips for pipette
- PATHFAST Tips
- Empty Reagent Cartridges
- Detection Wells
- Disposable plastic gloves
- 0.6ml tubes (for dispense check)

Note) Waste materials shall be discarded according to the regulation, because they
should be treated as potentially infectious materials.

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 PATHFAST SERVICE MANUAL Chapter 10

10 – 2 Cleaning

Begin maintenance by thoroughly cleaning the instrument.

10 – 2 – 1 Cleaning of outside instrument

The following photo and labels are referred to throughout this manual.

Status
Indicator

LCD Display
Front Cover
with Touch
Panel

Operation
Panel Cover

Start Button,
Stop Button

Printer
Cover
Front Lower
Cover

CLEANING
Note: For most cleaning procedures, use common wipe or towel moistened
(not dripping) with ethanol.

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PATHFAST SERVICE MANUAL Chapter 10

１．Front Cover 2. Front Lower Cover

Wipe with wipe moistened (not dripping) with ethanol.

3. Operation Panel Cover, LCD Display 4. Printer

Wipe operation panel cover and LCD display with wipe moistened (not dripping)
with ethanol, wipe paper dust off of rubber roller.

5. Start Button and Stop Button

Check that those buttons return to the original position.

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PATHFAST SERVICE MANUAL Chapter 10

6. Top of instrument and both side covers

Wipe with wipe dipped with ethanol.

7. Air filter on the bottom (Replace once per year, or as needed)

Place the spacers under the four feet of the instrument. Unscrew the thumb screw
fixing the filter cover.

Thumb Screw

Spacers

Replace filter with new one and place the filter cover back into place.

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PATHFAST SERVICE MANUAL Chapter 10

8. Air Vents on the rear of the instrument

Remove dust using tooth brush.

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 PATHFAST SERVICE MANUAL Chapter 10

10 – 2 – 2 Inside of Instrument

Open the front cover to can see the parts as shown in the following picture.

P-Axis Cover

Piercing Unit

Heat Block T
Magnet Unit

Heat Block S Waste Tip

Sensor
Heat Block L

Tip Holder

Follow the instruction below to clean the inside of instrument.

Note: For most cleaning procedures, use wipes or a towel moistened (not
dripping) with ethanol.
The inside of the instrument and Reagent Cartridge Rack should be
treated as if they were contaminated by samples or reagents. Follow
universal precautions for handling.

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PATHFAST SERVICE MANUAL Chapter 10

1. Reagent Cartridge Rack

Clean the rack and check for the looseness. If necessary, readjust and fix the
screws using the jig, six pieces. (Refer to the pictures below.)

2. Tip Waste Box

Ask the user to replace when it is dirty.

3. Sample Recognition Sensor

Use a cotton swab to clean up adhered dust or liquid.
.

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PATHFAST SERVICE MANUAL Chapter 10

4. Stage
Clean the metallic plate using towelette moistened with ethanol.

5. Heat Block S and Heat Block L

Clean the blocks using towelette moistened with ethanol. To clean the wells, use
cotton swab moistened with ethanol.

6. Heat Block T and Magnet Unit

Clean the block using towelette moistened with ethanol. To clean the magnets,
use cotton swabs moistened with ethanol.

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PATHFAST SERVICE MANUAL Chapter 10

7. Piercing Unit
Remove the P-Axis Cover by first removing the two fixing screws. Then clean the
Piercer ends using gauze moistened with ethanol. If the arm of Stopper against
Rack lifting is bent, replace the Stopper arm as needed.

8. Syringe Unit (Replace it once in three years)

Clean the nozzles using gauze moistened with ethanol. Clean carefully to remove
any liquid adhered to the nozzle end.
Note: When you find the evidence of liquid adhered in the past, report it to the
user. Though the performance tests on the syringes are successful, inform
the user that it may be needed to replace in the near future.

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PATHFAST SERVICE MANUAL Chapter 10

9. Under Stage (front and rear) 10. Both inner sides

Clean up using wipe moistened with ethanol.

11. PMT Unit, PMT Light Guide

Check for dust or dirt on Light Guide, using mirror.
Clean with dry cotton swab (without ethanol), when needed.

12. Barcode Reader 13. Used Tip Sensor

Check for dust or dirt on Barcode Reader and clean using wipe moistened with
ethanol as needed. Clean the sensor on both sides with cotton swab without
ethanol, when needed.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 3 Teaching Check

Teaching Check in periodic maintenance is performed visually.

Spec: Each teaching position is correct (Visual check)

Use maintenance CF card and refer to Chapter 7 Maintenance CF and Teaching

Operation for details.
Adjust as needed.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 4 Sensor Check

Spec: No abnormality
No abnormal sound is heard during operation

<Procedure of Sensor Check>

1. Replace the CF card with Maintenance CF card and turn the instrument power
on.
When MAINTENANCE MENU is displayed, select SELF DIAGNOSTIC.
In SELF DIAGNOSTIC screen, select START to start automatic checks for the
sensors.

When the test is finished successfully, “OK” is shown on the screen. Select
RETURN to go back to MAINTENANCE MENU.

Sensors checked by SELF

DIAGNOSTIC

YORG / Y＋EL
ZORG / Z＋EL
PORG / P＋EL
MORG
PXORG / ＋EL
PZORG
PMT SHUTTER CLOSE
PMT SHUTTER OPEN
PMT Z DOWN
COVER LOCK
TIP DETECT
COVER LOCK SOL

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PATHFAST SERVICE MANUAL Chapter 10

When test was finished and “NG” was indicated, check of the applicable point is
necessary.

Sensor Reference
YORG / Y＋EL 5-1-2
ZORG / Z＋EL 5-1-2 , 4-6-2
PORG / P＋EL 5-1-2 , 4-8 , 4-6-1
MORG 5-1-2 , 4-9
PXORG / ＋EL 5-1-2
PZORG 5-1-2
PMT SHUTTER CLOSE 5-1-2 , 4-11-2
PMT SHUTTER OPEN 5-1-2 , 4-11-2
PMT Z DOWN 5-1-2 , 6 of 4-11-2
COVER LOCK 5-1-2 , 4-10-2
TIP DETECT 5-1-2 , 4-7-3
COVER LOCK SOL 5-1-2 , 4-10-1

2. Next perform manual sensor check. First, select SENSOR MONITOR in

MAINTENANCE MENU to show SENSOR MONITOR screen.

・Start Button
Press Start Button on the instrument and check if the SWITCH START is
displayed in reverse.
・Stop Button
Press Start Button on the instrument and check if the SWITCH STOP is
displayed in reverse.
・Buzzer
Select BUZZER on the screen and check if the buzzer sound is heard. To
stop buzzer, press BUZZER again.
・Status Indicator

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PATHFAST SERVICE MANUAL Chapter 10

Select LED GREEN on the screen and check if the Status Indicator on the
instrument is lit in green.
Select LED RED on the screen and check if the Status Indicator on the
instrument is lit in red.

・Front Cover Sensor

First be sure that the left side cover is closed.
Select PMT5V ON/OFF on the screen to be displayed in reverse. PMT5V ON
is displayed in reverse showing the power is supplied.
Manually open/close Front Cover and check PMT5V ON is displayed in
normal or in reverse accordingly.

Sensors checked manually:

SWITCH START
SWITCH STOP
BUZZER
LED GREEN
LED RED
FRONT COVER SENSOR

The check of the applicable point is necessary when manual sensor check was
finished and there was NG.

Sensor Reference
SWITCH START 5-5-4
SWITCH STOP 5-5-4
BUZZER
LED GREEN 5-5-4
LED RED 5-5-4
FRONT COVER SENSOR 5-1-2

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PATHFAST SERVICE MANUAL Chapter 10

10 – 5 Sample Recognition Sensor Check

Spec: AD Value, 800∼1020 w/o tips

* Check this after more than 30 minutes from instrument power on.

・Select SAMPLE DETECTER in MAINTENANCE MENU.

Select ON for LED to read AD values without tips. Check the displayed AD
values if they are in the specified range.

Check it again when results are not within specification.

Replace unit in case of the same result.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 6 PMT Check

Spec:
・LED Count Ratio : 50 - 110
・Dark Count with Detection Well : less than 100 for the average of 24
reads for Hi
・LED Count for Hi : 900,000 – 1,100,000 cps

<Procedure of PMT Check>

・Set 6 empty reagent cartridges in Reagent Cartridge Rack and put it on the
instrument.
Use new detection wells confirming nothing is inside.

・ Select PMT OPERATION in MAINTENANCE MENU to show PMT

OPERATION screen. Then select NEXT in the screen to show PMT
OPERATION2 screen.

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PATHFAST SERVICE MANUAL Chapter 10

・ In PMT OPERATION2 screen, select LED MESUREMENT to make PMT

reading at its home position. Check the read values against specification.

Next select DARK MESUREMENT to read dark count in detection wells.

Check if the displayed values meet with specifications.

See 5-5-4<PMT Calibration Unit> when result of test is NG.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 7 Temperature Check

Check the temperature of each heat block of PATHFAST.

Spec:
・Temperature Test
Heat Block L 37.5℃±0.5℃
Heat Block S 65℃±2℃
Heat Block T 35℃±1.0℃
CHAMBER Temperature is displayed.
* Measure temperature after 30 minutes from instrument power on
with Front Cover closed.

・Select TEMP MONITOR in MAINTENANCE MENU screen.

Set the parameters as SCAN TIME ＝ 1s NUMBER OF TIMES ＝ 10 and
select MEASURMENT to read the temperature. Check the values shown
against spec.

・Remove the cover on the back and warm the Thermostat with your hand and
check if the Fan starts running.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 8 Leakage Check

Check that no leakage is found when Syringe Unit with Disposable Tips on
aspirated liquid.

Spec: Liquid levels in all tips do not change more than 3mm after 3 minutes
from aspiration of liquid.

・ Manually pipette 700 ul of DW in Well #12 for 6 empty cartridges.

・ Select RUN PROTOCOL in MAINTENANCE MENU screen.

Then select “Leak_Test.scr” from the dropped down list by selecting protocol
display box. Then select Exec.

・At the beginning of the leakage check, put marks for the liquid levels using oil
pen. When 3 minutes passed, put marks again for the liquid levels. Check that
the difference of the two marks is less than 5mm for all tips.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 9 Dispense Check

Check the dispensing accuracy and reproducibility of Syringe Unit of

PATHFAST.

Spec: 50μl CV ≦ 3%
Acc ≦ -8% - +2%
Reps 3

・ Pipette 100 ul of DW in Well#1 of 6 empty reagent cartridges.

Set 0.6ml tubes on the jig specially prepared for this test and set the jig at
Sample Recognition Sensor.

Jig for Dispense

Check

・Select DISPENSE TEST in MAINTENANCE MENU screen.

In DISPENSE TEST screen, select “Abs_Asp_Disp_Test.scr” from the dropped
down list by selecting protocol display box. Then select Exec.

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PATHFAST SERVICE MANUAL Chapter 10

・After DW has been dispensed in the tubes, measure the volume of DW in each
tube according to the following procedure.
First adjust the scale of the pipette to 53ul.

・Aspirate all DW in 0.6ml tube.

(When air does not come in at the end of tip end, increase the volume by
increasing the scale of the pipette.)

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PATHFAST SERVICE MANUAL Chapter 10

DW 50ul

Air 3ul

・Rotate the pipette knob slowly in dispense direction until the DW reaches to
the tip end.
Read the scale of the pipette for the volume of DW aspirated. Repeat this
measurement for 6 tubes and record the results.

Ex) Record as 50.1ul

・ Input the recorded data in the DISPENSE TEST screen.

Selecting CALCULATE, it calculates CV and ACCURACY.

Check those results against specification.

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PATHFAST SERVICE MANUAL Chapter 10

Check it again when results are not within specification.

Replace Syringe Unit in case of the same result.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 10 Running Check with Reagents

Finally check the instrument performance by running CAL/QC assay using

actual reagents.
Spec.:
・No error observed
・No abnormality (Noise, vibration, etc) observed
・Liquid movement in tips is consistent
・Barcodes of reagent cartridges are read without error

・ Replace CF card with normal CF for customer use and turn the instrument
power on.
・ Set 6 reagent cartridges, 6 tips and Tip Waste Box on the instrument.
・ Check if the printer paper is enough.

・Pipette 100ul of CAL1 in sample wells of Lane #1 and #2.

・Pipette 100ul of CAL2 in sample wells of Lane #3 and #4.
・Pipette 100ul of QC1 in sample well of Lane #5 and QC2 in sample well of Lane
#6.

QC1 QC2

CAL1 CAL2
・Select CAL/QC Assay and select the assay name (TBD) to be run.
When the assay completed, check if the calibration assay passed and the data
for QC1 and QC2 are within the specified ranges.

Note) Lastly clean the piercer nozzles using wipe moistened with ethanol.

If results are not within specification, see 8-2 & 8-5.

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PATHFAST SERVICE MANUAL Chapter 10

10 – 11. Recording Instrument Information

Lastly record the instrument information of PATHFAST which you performed

periodic maintenance.

10 – 11 – 1. PATHFAST Software CF
1. Turn the instrument power on with PATHFAST Software CF at customer site
and select SYSTEM, INSTRUMENT INFO consecutively from ADMIN screen
to show the screen below.
INSTRUMENT INFO

2. Record serial number and program version in PM Check List.

10 – 11 – 2. Maintenance CF
1. Turn the instrument power on with Maintenance CF used for maintenance
work and select LOG MONITOR, INSTRUMENT INFO consecutively from
MAINTENANCE MENU screen to show the screen below.
INSTRUMENT INFO

2. Record program version, EXTBOARD VERSION and ASSAY COUNT in PM

Check List.

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 PATHFAST SERVICE MANUAL Chapter 10

10 – 12 PM Check List

PATHFAST PM Check List 1/2

Date of PM Instrument S/N Service Engineer

O N
Item Spec., etc K G
Values Remark
Front Cover Cleaning
Lower Front Cover Cleaning
Operation Panel Cover Cleaning
Clean LCD Display Cleaning
up :
Printer Cleaning
Exterio
1.1 Start / Stop Buttons Smooth Movement
r of
instru Top of Instrument Cleaning
ment Both Side Covers Cleaning
Replace(yearly, or as
Air Filter
needed)
Air Vents on rear Cleaning
Reagent Cartridge Rack Cleaning
Waste Tip Box Cleaning
Sample Recognition Cleaning
Sensor
Stage Cleaning
Heat Block L Cleaning
Clean Heat Block S Cleaning
up: Heat Block T Cleaning
Interio
1.2 Magnet Cleaning
r of
instru Piercing Unit Cleaning
ment Syringe Unit Cleaning
Under Stage, bottom of Cleaning
instrument
Both Inner Side Walls Cleaning
PMT Shutter Unit Cleaning
Barcode Reader Cleaning
Waste Tip Sensor Cleaning
@TP.Y.BlackWell
@TP.Y.TipOn
@TP.Y.PMT
@TP.Y.SampleDetector
@TP.Z.Proc.SmallWell
Teachi
@TP.Z.Proc.LargeWell Within the Acceptable
2.0 ng
@TP.Z.Proc.BlackWell Range
Check
@TP.Z.Tip(1).Standby
@TP.Z.PiercingProcess
@TP.M.BasePos
@TP.PX.PMT(1).Center
@TipTrashSense.Y.Center

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 PATHFAST SERVICE MANUAL Chapter 10

PATHFAST PM Check List 2/2

Date of PM Instrument S/N Service Engineer

O N
Item Spec., etc K G
Values Remark
SELF DIAGNOSTIC No Failure
Start / Stop Buttons Normal Operation
Sensor Buzzer Sounds
2.1 Check Status Indicator Lit in green or red
Lower Front Cover Normal Operation
Front Cover Sensor Normal Operation
Lane#1: AD Value
Sample Lane#2: AD Value
Recogni Lane#3: AD Value AD Value:
2.2
tion Lane#4: AD Value 800 - 1020
Sensor Lane#5: AD Value
Lane#6: AD Value
LED Count Ratio (Hi/Lo) H/L: 50 - 110
PMT Dark Count in Detection Average count for Hi
2.3
Check Wells (24) is less than 100
LED Hi Count
Average: Heat Block L ℃

Max.: Heat Block L 37.5℃ ±0.5℃ ℃

Min.: Heat Block L ℃
Average: Heat Block S ℃
Tempera
Max.: Heat Block S 65.0℃ ±2.0℃ ℃
2.4 ture
℃
Check Min.: Heat Block S
Average: Heat Block T ℃

Max.: Heat Block T 35.0℃ ±1.0℃ ℃

Min.: Heat Block T ℃

Chamber(Thermostat) Normal Operation

Leakage Less than 3mm changes for
2.5 Leakage Check all tips after 3 minutes
Check
CV≦3%
Dispens Ref. to Attachment: Dispense
2.6 50 ul Dispensing Acc≦-8 - +2% Check Table
e Check
(Run Rep. of 2)
Running ・No Error
・No abnormality
Check
Performance Check by ・Consistent liquid
3.0 with
running CAL/QC assay movement
Reagent ・No Barcode Read Error
s ・Cleanup of Piercers
Serial Number
Instrum PATHFAST Software CF
Program Version
ent
4.0 Program Version
Informa
tion Maintenance CF Extboard Version
Assay Count

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 PATHFAST SERVICE MANUAL Chapter 10

Attachment: Dispense Check Table

Date of PM Instrument S/N Service Engineer

Repetition Accura
CV Remark
cy
1 2 3

Lane #1

Lane #2

Lane #3

Lane #4

Lane #5

Lane #6

CV

Accuracy

Spec: 50μl CV ≦ 3%
Acc. ≦ -8％ - +2%(Against 100%)
Reps. 3 times

Judge : OK, NG

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FOR FURTHER INFORMATION:

Mitsubishi Kagaku Iatron, Inc.

13-1, Nishi-Goken-cho, Shinjuku-ku, Tokyo 162-0812 Japan

Mitsubishi Chemical Europe GmbH

Prinzenallee 13 40549 Duesseldorf Germany