Utah State University provides teaching materials for genetic engineering topics through its "Genetic Engineering Workshop for Teachers" program. The document outlines 7 lessons on biotech basics, including defining terms like biotechnology, genetic engineering, recombinant DNA, and CRISPR. Feedback on the materials can be directed to graduate student Olivia Horning.
Utah State University provides teaching materials for genetic engineering topics through its "Genetic Engineering Workshop for Teachers" program. The document outlines 7 lessons on biotech basics, including defining terms like biotechnology, genetic engineering, recombinant DNA, and CRISPR. Feedback on the materials can be directed to graduate student Olivia Horning.
Utah State University provides teaching materials for genetic engineering topics through its "Genetic Engineering Workshop for Teachers" program. The document outlines 7 lessons on biotech basics, including defining terms like biotechnology, genetic engineering, recombinant DNA, and CRISPR. Feedback on the materials can be directed to graduate student Olivia Horning.
Utah State University provides teaching materials for genetic engineering topics through its "Genetic Engineering Workshop for Teachers" program. The document outlines 7 lessons on biotech basics, including defining terms like biotechnology, genetic engineering, recombinant DNA, and CRISPR. Feedback on the materials can be directed to graduate student Olivia Horning.
Genetic Engineering This is a project resulting from the “Genetic
Engineering Workshop for Teachers” to provide
in Agriculture teaching materials for genetic engineering topics.
Please direct any feedback to ASTE graduate student
Olivia Horning at olivia.horning@usu.edu
START COURSE
1 Lesson 1: Biotech Basics Gavrilescu, M. (2010). Environmental biotechnology: achievements, opportunities and challenges. Dynamic biochemistry, process biotechnology and molecular biology, 4(1), 1-36.
2 Lesson 1: Biotech Basics 1. Define biotechnology and DNA technology. 2. Define genetic engineering and list common terms used to describe the organisms created through genetic engineering. 3. Illustrate the process of recombinant DNA and explain each step. 4. Differentiate between CRISPR and transgenic technology. Activity: Yeast Cells Respire Too, How to make yeast cells thrive Show the following video from the National Collection of Yeast Cultures - https://www.youtube.com/watch?v=Wvd1zCWu8nY
4 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 • Biotechnology: A number of methods
Lesson 2 other than selective breeding and sexually crossing plants to endow new Lesson 3 characteristics in organisms (NAS, 2016) Lesson 4 • Manipulating organisms or organism Lesson 5 parts to create a product. • Yeast in bread and beer production Lesson 6 • Selectively breeding livestock. Lesson 7 • DNA technology: Laboratory techniques to analyze genetic material in order to study and manipulate. 5 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 • Genetic engineering: The introduction or change of DNA, RNA,
Lesson 2 or proteins by human manipulation to effect a change in an organism’s genome or epigenome (NAS, 2016) Lesson 3 • Practical and direct manipulation of genes, like food products and chemicals (cancer drugs, pesticides, etc.) Lesson 4 • Specific genes can be modified and moved between organisms (plants, Lesson 5 animals, and bacteria). • Organisms that have one or more genes by artificial means Lesson 6 using DNA technology have many terms: Genetically Lesson 7 modified/Genetically modified organism – GM or GMO, Transgenic organism, Bioengineered
6 Interest Approach: DIY bio Visit the following website http://sphere.diybio.org as a class and discuss their philosophy, projects, and lab locations.
7 Your project title Biotech Basics goes here
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
https://www.gene.com/st ories/biotech-basics Genetic Engineering in Biotech Basics Agriculture
Lesson 1 Recombinant DNA: Any novel DNA sequence created using
Lesson 2 genetic engineering (NAS, 2016) Lesson 3 Transgenic: An organism that has had genes that contain sequences from another species or synthetic sequences Lesson 4 introduced into its genome by genetic engineering (NAS, 2016) Lesson 5 • Two different nucleotide sequences from two different sources form a DNA molecule when combined. Lesson 6 • Graphic source: Khan Academy Lesson 7
9 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 Plasmid Vector: A circular DNA molecule endogenous to bacteria
Lesson 2 that is used to replicate and transfer DNA to new cells (NAS, 2016) Lesson 3
Lesson 4 • Bacterial plasmids are separate from the bacterial Lesson 5 chromosome. • Smaller and circular in shape. Lesson 6 • Incorporates foreign DNA easily. Lesson 7 • Can be taken up by bacterial cells. • Used for gene cloning • Gene cloning animation and diagram 10 Genetic Engineering in Biotech Basics Agriculture
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
11 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 Restriction Enzymes
Lesson 2
Lesson 3 • Recombinant DNA is made up of a bacterial plasmid and gene
of interest Lesson 4 • Bacterial enzymes cut and paste DNA, known as restriction Lesson 5 enzymes. Lesson 6 • Restriction site, the specific part of a DNA sequence that is recognized by a restriction enzyme Lesson 7 • Restriction enzyme animation • Graphic source: Khan Academy 12 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 Sticky Ends
Lesson 2
Lesson 3 • Sticky ends are created after a strand of DNA is cut by a
restriction enzyme, the sticky ends are single stranded DNA or Lesson 4 RNA ends that have a nucleotide base sequence that is Lesson 5 complementary to another strand, allowing them to connect through base paring. Lesson 6 • Graphic source: Khan Academy Lesson 7
13 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 DNA Ligase
Lesson 2
Lesson 3 • Joined by DNA ligase, from fragments to strands. It is the glue
for the DNA pieces Lesson 4 • Hundreds of restriction enzymes exist, originally for making Lesson 5 sure foreign DNA did not invade the bacterial cell. Lesson 6 • Scientists can multiply proteins using this process in a bacterium, yeast, or other cell. Lesson 7 • Graphic source: Khan Academy
14 Genetic Engineering in Biotech Basics Agriculture
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
15 Genetic Engineering in Biotech Basics Agriculture
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
16 Genetic Engineering in Biotech Basics Agriculture
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
17 Genetic Engineering in Biotech Basics Agriculture
Lesson 1
Lesson 2
Lesson 3
Lesson 4
Lesson 5
Lesson 6
Lesson 7
Summarize with the following video on the recombinant DNA
process: https://www.youtube.com/watch?v=FAMRQz7fOaE 18 Interest Approach: CRISPR Kit Evaluate the CRISPR DIY Bio kit as a class at http://www.the-odin.com/diy-crispr- kit/ and emphasize how accessible this technology is becoming. Discuss whether this is a good thing or not?
19 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 CRISPR: A naturally occurring mechanism of immunity to viruses
Lesson 2 found in bacteria that involves identification and degradation of foreign DNA. This natural mechanism has been manipulated by Lesson 3 researchers to develop gene editing techniques (NAS, 2016) Lesson 4
Lesson 5 • CRISPR-Cas9 technology allows genes to be cut out, replaced, or
added to the sequence = simple, versatile, precise Lesson 6 • CAS9 is a protein that comes from the immune system of bacteria Lesson 7 that recognizes viruses, in order to cut up the viral DNA in order to fight viruses off, very similar to human antibodies. • CRISPR CAS9 is programmable to recognize any DNA sequence and can cut at specific DNA sequence sites like at a restriction enzyme. 20 Genetic Engineering in Biotech Basics Agriculture
Lesson 1 CRISPR Lesson 2
Lesson 3 • CRISPR and a new gene are introduced to a cell through viral vectors, genetically engineered viruses. Lesson 4 • CRISPR makes the cut, the DNA is inserted, and then spliced Lesson 5 together to become fully functional. Lesson 6 • A gene is “fixed” through this process of gene therapy. Lesson 7 • Hemophilia, Cystic Fibrosis, Immune Deficiencies, Hereditary blindness, blood disease (e.g. sickle cell anemia), Cancer (e.g. melanoma, leukemia), Parkinson’s Disease, and more.
21 Activity: Pick a Podcast Using the provided podcast worksheet, evaluate the first podcast (General) as a class and then allow students practice individually with podcast 2 or 3 depending on the class: 1. (General) A CRISPR Way to Fix Faulty Genes https://www.npr.org/sections/health- shots/2014/06/26/325213397/a-crispr-way-to-fix-faulty-genes 2. (Plant Science) Amid GMO Strife. Food Industry Vies for Public Trust in CRISPR Technology https://www.npr.org/sections/thesalt/2017/10/25/559867742/amid-gmo-strife-food-industry- vies-for-public-trust-in-crispr-technology 3. (Animal Science) CRISPR Bacon: Chinese Scientists Create Genetically Modified Low-Fat Pigs https://www.npr.org/sections/thesalt/2017/10/23/559060166/crispr-bacon-chinese-scientists- create-genetically-modified-low-fat-pigs
22 Genetic Engineering in Content Source Agriculture
Lesson 1 • Pearson Education
Lesson 2 • Campbell Essential Biology (6th Ed.) by Simon, Reece, & Dickey. Lesson 3 • National Academies of Sciences, Engineering, and Lesson 4 Medicine. 2016. Genetically Engineered Crops: Experiences Lesson 5 and Prospects. Washington, DC: The National Academies Press. https://doi.org/10.17226/23395. Lesson 6
