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Enzyme Basics Practice KEY 2hg9sph

The document provides information about enzyme basics including the lock and key model of enzyme-substrate binding, enzyme functions such as catalysis, and properties including substrate specificity and reusability. It discusses enzyme names ending in -ase and being named after their substrate. It also covers factors that affect the rate of enzymatic reactions like temperature, pH, and enzyme concentration. Specifically, raising temperature slightly or adjusting pH to optimal levels increases reaction rate while freezing decreases it. The document examines the enzymes pepsin and pancreatic amylase, which function in the stomach and small intestine, respectively. It analyzes a graph of enzyme activity at different temperatures.

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Avy Danielle G. Rodriguez
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582 views4 pages

Enzyme Basics Practice KEY 2hg9sph

The document provides information about enzyme basics including the lock and key model of enzyme-substrate binding, enzyme functions such as catalysis, and properties including substrate specificity and reusability. It discusses enzyme names ending in -ase and being named after their substrate. It also covers factors that affect the rate of enzymatic reactions like temperature, pH, and enzyme concentration. Specifically, raising temperature slightly or adjusting pH to optimal levels increases reaction rate while freezing decreases it. The document examines the enzymes pepsin and pancreatic amylase, which function in the stomach and small intestine, respectively. It analyzes a graph of enzyme activity at different temperatures.

Uploaded by

Avy Danielle G. Rodriguez
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Name _______________________________________________

ENZYME BASICS PRACTICE


Lock and key model:
1. Label the diagram using the words: active site, products, enzyme, substrate

SUBSTRATE PRODUCT

ACTIVE SITE

e._______________
ENZYME-
SUBSTRATE
______
COMPLEX
ENZYME

ENZYME FUNCTIONS:

2. Match the following words with their definitions:

E Product D Active site B Enzymes F Catalyst

C Substrate A Activation Energy

a. Amount of energy required for a chemical reaction to occur


b. Substances that bring about a chemical reaction without being changed itself
c. Substance that an enzyme changes into a product
d. Regions on surfaces of enzymes that fit the substrate
e. Substance formed from the substrate at the end of a chemical reaction with a an enzyme
f. Proteins that speed up chemical reactions

ENZYME PROPERTIES:

Answer true of false to the following statements:

a. T Enzymes interact with specific substrates


b T Enzymes speed up reactions.
d. F One enzyme can be used for many different types of chemical reactions.
e. T Enzymes are re-used after a reaction.
ENZYME NAMES:
3. Enzyme names end with ASE
4. Enzyme are named after their SUBSTRATE Example LIPASE, PROTEASE, AMYLASE

ENERGY OF ACTIVATION:

5. What is the energy of activation? THE ENERGY NEEDED FOR REACTANTS TO BECOME
PRODUCTS

6. Refer to the graph of the reaction pathway without an enzyme to the right. Draw a line on the graph
that shows the reaction pathway if an enzyme is added to the reactants.

FACTORS THAT AFFECT THE ENZYME RATE OF REACTION

7. Circle the correct effect.

a. Raising the temperature slightly will [ increase | decrease | not change ] the rate of reaction
b. Keeping the pH around the optimal pH will [ increase | decrease | not change ] the rate of reaction.
d. Increasing the salinity of solution will [ increase | decrease | not change ] the rate of reaction.

8. Place a check mark next to the things that are expected to INCREASE the rate of an enzymatic
reaction

a. ____X___ Add more enzyme


b. _______ Add more substrate
c. ____X___ Adjust pH to optimal level
d_______ Add a non competitive inhibitor
e._______ Freezing
8. The enzyme pancreatic amylase is manufactured and secreted by the pancreas into the small
intestine. Pancreatic amylase breaks down starch into a smaller sugar. Pepsin is an enzyme that is
released by the stomach and functions to break down proteins into amino acids.
The following graph shows the pH at which both pepsin and pancreatic amylase can function in the body.
If the pH of the body falls above or below the graphs for each enzyme respectively, that enzyme will
denature and no longer function. The higher the curve of the graph, the more productive the enzyme.

Which of the following statements is true with respect to


Figure 1?
a) T or F : Pepsin and pancreatic amylase could
never function together in the same part of the
body at the same time.
b) T or F : Pancreatic amylase could function in the
stomach with a pH of 1-2.
c) T or F : The optimal pH for the functioning of
pepsin is approximately 8.5 to 9.
d) T or F: Pancreatic amylase is used in the small
intestine. Normally, the small intestine must be slightly acidic in order for it to function.

9) Use the graph to the right to answer the


questions below.

A. Figure 2 depicts the activities of three


enzymes. Remembering that the average
human body temperature is 98.6oF (37oC).
Which curve illustrates the functioning of
human DNA polymerase, which functions in the
nucleus of cells?
a. Enzyme A
b. Enzyme B
c. Enzyme C
d. None of the above could represent the
activity of human DNA polymerase.

B.Which curve illustrates the functioning of DNA polymerase from a shark (Hint: the body
temperature of a shark is typically the same as water that surrounds it)(water temperature
average is 10 degrees C)?
e. Enzyme A
f. Enzyme B
g. Enzyme C
h. None of the above, since sharks, like all fish, do not contain DNA polymerase.

C.At what temperature would enzyme B be completely denatured?


i. 37°C
j. 15°C
k. 25°C
l. 50°C

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