BIO 217

AP2 Week 3 Lab Report

Blood-Introduction to Hematology Lab

Introduction: In this week’s labster lab, we learned about hematology. Hematology is

the study of blood and blood disorders. A hematologist is concerned with the study, diagnosis,
treatment, and prevention of disease related to blood. In this lab, we learn about different blood
components. We were taught how to make peripheral blood smears and generate complete blood
count.
Procedure:
 Materials: Labeled blood collection tubes, automated hematology analyzer,
scanner, glass slides, absorbing paper, fixative, Giesme, distilled water, immersion
oil, pipette, and a microscope.
 Methods: We scanned labeled blood samples with a purple cap, inverted the tube,
and inserted it into the scanner to be analyzed and we observed the results. Then
we used the fixative and checked stained blood control samples under a
microscope for red and white blood cells and platelets. Then we placed one drop
of blood onto the microscopy slide with a pipette, spread out the blood drop with
another microscopy slide, and dried the blood smear on absorbing paper. Then we
put it in the fixative for 30 seconds, then we put it in a Giemsa stain for 2 minutes
and then washed it with distilled water and air-dried it on absorbing paper. Then
we placed the blood smear and staining on the microscopy stage for analysis.
Plasmodium falciparum

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 Sickle cell anemia

Hemophilia

Results: We took blood samples of three patients and these are the results.
PATIENT #1: Mrs. Amatore returned from her travels to Tanzania with recurring fever and chills.
These are very typical signs of an infection with Plasmodium falciparum-an intracellular parasite
causing malaria in patients. We detected the characteristic ring stage of parasites inside the
patient’s blood cell and measured an increased white blood cell, indicative of an infection.
Tanzania is in fact an endemic area for Plasmodium falciparum.
PATIENT #2: A 10-month-old boy, we detected abnormally shaped red blood cells in the blood
smear of the patient’s blood that is a very clear sign of sickle cell anemia. In fact, the name of the
disease is based on the abnormality in the blood vessels, leading to painful swelling of joints and
anemia. In support of this we detected a slight anemia in the complete blood count (CBC),
reflected by low hemoglobin (Hb) hematocrit (HCT) and corpuscular volume (MCV) values.
PATIENT #3: A 2-year-old. It was a tricky case so far. We could not find any abnormalities in
the complete blood count (CBC) and the peripheral blood smear appeared to be normal.

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 However, the patient showed very typical signs of bleeding disorder, such as easy bruising and
bleeding without any cause.

Week 3 Virtual Simulation Labs:

In the week 3 labs, we looked at the blood vessels with lab models and a dissection of a cat.

In the lungs, the pulmonary arteries (in blue) carry deoxygenated blood from the heart into the

lungs. Throughout the body, the arteries (in red) deliver oxygenated blood and nutrients to all the

body’s tissues, and the veins (in blue) return oxygen-poor blood back to the heart.

The aorta is the large artery leaving the heart. The superior vena cava is the large vein that brings

blood from the head and arms to the heart, and the inferior vena cava brings blood from the

abdomen and legs into the heart.

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 Arteries (in red) are the blood vessels that deliver blood to the body.

Veins (in blue) are the blood vessels that return blood to the heart.

Deep veins, located in the center of the leg near the leg bones, are enclosed by muscle. The iliac,

femoral, popliteal and tibial (calf) veins are the deep veins in the legs.

Superficial veins are located near the surface of the skin and have very little muscle support. The

great saphenous vein is a superficial vein.

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 Then we looked at blood. Blood is a connective tissue. The only liquid tissue in the body. In

blood there is plasma (the true liquid in blood) and the formed elements(red blood cells, red

blood cells and platelets).

Antigens are substances that cause your immune system to produce antibodies against them.

Antibodies are blood proteins produced in response to and counteracting specific antigens.

We looked at the cardiovascular and circulatory histology and the blood vessel histology.

We looked at microscopic slides for blood cells too.

We explored and learned about pulmonary veins and arteries.

Conclusion: By watching the stimulation videos in the lab, we learned that the

pulmonary arteries carry deoxygenated blood from the heart into the lungs. Throughout the body,

the arteries deliver oxygenated blood and nutrients to all the body’s tissues, and the veins return

oxygen-depleted blood back to the heart. The superior vena cava is the large vein that brings

blood from the head and arms to the heart, whereas the inferior vena cava brings blood from the

abdomen and legs into the heart. We also looked at blood as a form of a connective tissue plus its

elements. In the labster we caught on hematology which is the study of blood and its disorders.

Resources:

https://api2.labster.com/ref/837f88ef97e2444/simulation/a0Kw000000UYOpiEAH/

https://www.google.com/search?q=hemophilia+blood+smear

https://www.google.com/search?q=images+of+malarial+parasite

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