Enzyme Data
Enzyme Data
Enzyme Data
4864
0.0062 0.207 0.3111 0.4103
0.0884 0.175 0.2453 0.2886
0.097 0.238 0.2356 0.2309
0.1131 0.1571 0.1968 0.2277
0.1015 0.1666 0.198 0.2281
Absorbance (401nm)
0.4 R² = 0.996466820214703
20.1 0.1131 0.1571 0.1968 0.2277 1.0143
20.2 0.1015 0.1666 0.198 0.2281 0.8928 0.3
0.2
0.1
0
0 2 4 6
Time (
10 Fold (1
0.35
0.3
f(x) = 0.013418 x + 0.09869
Absorbance (401nm)
0.25 R² = 0.979353725529446
0.2
0.15
0.1
0.05
0
0 2 4 6 8
Time (m
20 Fold (
0.25
0.15
0.1
0.05
f(x) = 0.00767 x + 0.11615
0.2 R² = 0.994063557046594
Absorbance (401nm)
0.15
0.1
0.05
0
0 2 4 6
Time (
5 fold -1
0.6 0.45
0.4 f(x) = 0.13164 x
0.5 R² = 0.9665132
f(x) = 0.14413 x − 0.0815 0.35
R² = 0.996466820214703
0.4 0.3
0.25
0.3
0.2
0.2 0.15
0.1
0.1
0.05
0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5
10 fold - 1
0.35 0.25
f(x) = 0.0399
R² = 0.55847
0.3
f(x) = 0.06709 x + 0.0316 0.2
R² = 0.979353725529446
0.25
0.2 0.15
0.15
0.1
0.1
0.05
0.05
0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5
20 fold - 1
0.25 0.25
f(x) = 0.03835 x + 0.0778 f(x) = 0.04112 x +
0.2 R² = 0.994063557046594 R² = 0.959294497
0.2
0.15 0.15
0.1 0.1
0.05 0.05
0 0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 0.5 1 1.5
5 Fold (1) 5 Fold (2)
0.45
0.4 f(x) = 0.026328 x + 0.03619
28826 x + 0.06263 R² = 0.966513247169886
0.35
Absorbance (401nm)
6466820214703
0.3
0.25
0.2
0.15
0.1
0.05
4 6 8 10 12 14 16 0
0 2 4 6 8 10 12 14
Time (min) Time (min)
0.15
0.1
0.05
4 6 8 10 12 14 16 0
0 2 4 6 8 10 12 14
Time (min) Time (min)
0.15
0.1
0.05
.00767 x + 0.11615 f(x) = 0.008224 x + 0.11187
994063557046594 0.2 R² = 0.959294497566666
Absorbance (401nm)
0.15
0.1
0.05
0
2 4 6 8 10 12 14 16 0 2 4 6 8 10 12 14
Time (min) Time (min)
5 fold -2
10 fold - 2
.25
f(x) = 0.03993 x + 0.10055
R² = 0.558475916062841
0.2
.15
0.1
.05
0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
Chart Title
8 10 12 14 16
min)
(2)
8 10 12 14 16
(min)
(2)
8 10 12 14 16
(min)
pH 0 10 slope pH slope
3.5 Trial 1 0.0684 0.0867 0.00183 3.5 0.00183
3.5 Trial 2 0.0708 0.0868 0.0016 3.5 0.0016
5.5 Trial 1 0.176 0.4063 0.02303 5.5 0.02303
p
5.5 Trial 2 0.156 0.3182 0.01622 5.5 0.01622
7.5 Trial 1 0.2854 1.3838 0.10984 7.5 0.10984 0.12
V0
0.04
0.02
0
3 3.5 4 4.5
pH 3.5 Assay 1
0.1 0.1
0.09 0.09
0.08 0.08
Absorbance (401nm)
Absorbance (401nm)
0.07 0.07
0.06 0.06
0.05 0.05
0.04 0.04
0.03 0.03
0.02 0.02
0.01 0.01
0 0
0 2 4 6 8 10 12 0
Time (mins)
pH 5.5 Assay 1
0.45 0.35
0.4
0.3
0.35
Absorbance (401nm)
Absorbance (401nm)
0.3 0.25
0.25 0.2
0.2 0.15
0.15
0.1
0.1
0.05 0.05
0 0
0 2 4 6 8 10 12 0
Time (mins)
0.25 0.2
Absorbance (
Absorbance
0.2 0.15
0.15
0.1
0.1
0.05 0.05
0 0
0 2 4 6 8 10 12 0
Time (mins)
pH 7.5 Assay 1
1.6 1.4
1.4 1.2
1.2
Absorbance (401nm)
Absorbance (401nm)
1
1
0.8
0.8
0.6
0.6
0.4
0.4
0.2 0.2
0 0
0 2 4 6 8 10 12 0
Time (mins)
3.5 -1
0.1
0.09
0.08 f(x) = 0.0183 x + 0.0501
R² = 1
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2
5.5-1
0.45 0.35
0.4
f(x) = 0.2303 x − 0.0543 0.3
0.35 R² = 1
5.5-1
0.45 0.35
0.4
f(x) = 0.2303 x − 0.0543 0.3
0.35 R² = 1
0.25
0.3
0.25 0.2
0.2 0.15
0.15
0.1
0.1
0.05 0.05
0 0
0.8 1 1.2 1.4 1.6 1.8 2 2.2 0.8 1
7.5-1
1.4
1.6
1.2 f
1.4
f(x) = 1.0984 x − 0.813 R
1.2 R² = 1 1
1 0.8
0.8 0.6
0.6
0.4
0.4
0.2
0.2
0
0 0.8 1
0.8 1 1.2 1.4 1.6 1.8 2 2.2
pH vs. V0
0.12
0.1
0.08
0.06
0.04
0.02
0
3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8
pH
pH 3.5 Assay 2
0.1
0.09
0.08
Absorbance (401nm)
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0 2 4 6 8 10 12
Time (mins)
pH 5.5 Assay 2
0.35
0.3
Absorbance (401nm)
0.25
0.2
0.15
0.1
0.05
0
0 2 4 6 8 10 12
Time (mins)
0.2
Absorbance (
0.15
0.1
0.05
0
0 2 4 6 8 10 12
Time (mins)
pH 7.5 Assay 2
1.4
1.2
Absorbance (401nm)
0.8
0.6
0.4
0.2
0
0 2 4 6 8 10 12
Time (mins)
3.5-2
0.1
0.09
0.08 f(x) = 0.016 x + 0.0548
R² = 1
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2
5.5-2
0.35
0.2
0.15
0.1
0.05
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2
7.5 -2
1.4
0.8
0.6
0.4
0.2
0
0.8 1 1.2 1.4 1.6 1.8 2 2.2
Temp 0.0000001 15 Slope
50 0.1731 0.3548 0.012113
60 0.1813 0.3393 0.869565 Temperature vs V0
70 0.1637 0.257 0.590506
1
0.9
0.8
0.7
V0 (mmol/min)
Temperature vs time 0.6
Temp 1E-07 15 Slope 0.5
50.000001 0.1731 0.3548 0.012113 0.4
60 0.1813 0.3393 0.869565 0.3
0.2
70 0.1637 0.257 0.590506
0.1
0
50 55 60 65
Temperature (°C)
Thermal Inactivati
0.4
0.35
f(x) = 0.0121133334140889 x + 0.1730
0.3 R² = 1
Absorbance (401mn)
0.25
0.2
0.15
0.1
0.05
0
0 2 4 6 8
Time (m
Thermal Inactivati
0.3
0.25
f(x) = 0.00622000004146667 x + 0.1
Absorbance (401mn)
R² = 1
0.2
0.15
0.1
0.05
0.25
f(x) = 0.00622000004146667 x + 0.1
Absorbance (401mn)
R² = 1
0.2
0.15
0.1
0.05
0
0 2 4 6 8
Time (m
emperature vs V0
60 65 70 75
Temperature (°C)
R² = 1
0.25
0.2
0.15
0.1
0.05
0
2 4 6 8 10 12 14 16 0 2 4 6 8 10 12
Time (min) Time (min)
= 0.00622000004146667 x + 0.163699999378
1
= 0.00622000004146667 x + 0.163699999378
1
2 4 6 8 10 12 14 16
Time (min)
ctivation (60°C)
6 x + 0.181299998946667
8 10 12 14 16
Time (min)
zoom out for more charts
a=ebc
Inhibition (18μL) 0 10 substrate conc abs/molar=conc slope 1/[s]
0 0.0189 0.014 0 7.61697497E-07 -0.00049
50 0.0265 0.0528 0.1375 2.8726877E-06 0.00263 7.272727
80 0.0255 0.078 0.22 4.2437432E-06 0.00525 4.545455
120 0.0445 0.0962 0.33 5.23394995E-06 0.00517 3.030303
170 0.0469 0.1596 0.4675 8.68335147E-06 0.01127 2.139037
230 0.0769 0.1287 0.6325 7.00217628E-06 0.00518 1.581028
300 0.0936 0.1898 0.825 1.03264418E-05 0.00962 1.212121
380 0.0734 0.0259 1.045 1.40914037E-06 -0.00475
470 0.0986 0.2418 1.2925 1.31556039E-05 0.01432 0.773694
570 0.1308 0.3383 1.5675 1.8405876E-05 0.02075 0.637959
680 0.1135 0.2776 1.87 1.51033732E-05 0.01641 0.534759
800 0.1339 0.2506 2.2 1.36343852E-05 0.01167 0.454545
slope
0.025
0.02
0.015
0.01
0.005
0
0 100 200 300 400 500 600 700 800 900
-0.005
-0.01
0
0 100 200 300 400 500 600 700 800 900
-0.005
-0.01
0.02
V0 (mmol/s)
0.005
0
0 100 200 300 400 500 600 700 800 900
Axis Title
0.02
V0 (mmol/s)
0.01
0.005
0
0 0.5 1 1.5 2 2.5
[S] (mM)
1/[product] Inhibition (6μL) 0 10 substrate c abs/molar=conc
0 = DNE 0 0
348106.1 50 0.0984 0.266 0.1375 1.4472252E-05
235641 80 0.1146 0.3006 0.22 1.6354733E-05
191060.3 120 0.1198 0.3742 0.33 2.0359086E-05
115162.9 170 0.1232 0.4001 0.4675 2.1768226E-05
142812.7 230 0.1297 0.5937 0.6325 3.2301415E-05
96838.78 300 0.1546 0.6454 0.825 3.5114255E-05
380 0.1646 0.7159 1.045 3.8949946E-05
76013.23 470 0.1701 1.0499 1.2925 5.7121872E-05
54330.48 570 0.2406 0.7823 1.5675 4.2562568E-05
66210.37 680 0.1951 0.9245 1.87 5.0299238E-05
73343.97 800 0.2663 0.8921 2.2 4.8536453E-05
0.07
f(x) = 0.0223923291036026
f(x) = 14608.4191141795 ln(x) + 0.054823025273403
x + 11428.7127283005
V0 (mmol/s)
0.06
0
0.05
0 0.5 1 1.5 2 2.5
0.04
1/[S]
0.03
0.02
0.01
0
0 0.5 1 1.5
2 3 4 5 6 7 8
[S] (mM)
1/[S]
Combined 18µL, 6
1/[s] 18ul 6ul 0ul
7.272727 348106.060606 69097.74 86494.118
400000
4.545455 235641.025641 61144.38 61553.918 350000
3.030303 191060.29106 49118.12 46122.961 f(x) = 41974.635317461 x + 46845.40
300000
2.139037 115162.907268 45938.52 20015.246 250000
1.581028 142812.742813 30958.4 96889.826 200000
1.212121 96838.7776607 28478.46 9132.0117 150000
0.956938 25673.98 84466.912 100000
0.773694 76013.2340778 17506.43 9432.8971 50000 f(x) = 7990.99441163438
4404.08239750869 x + 18817.7
44313.5
0.637959 54330.475909 23494.82 39006.791 0
0 1 2 3
18ul
Linear (6ul)
150000
100000
50000 f(x) = 7990.99441163438
4404.08239750869 x + 18817.7
44313.5
0
0 1 2 3
0.534759 66210.3746398 19881.02 91991.992
0.454545 73343.9744613 20603.07 44246.509 18ul
Linear (6ul)
V0 (mmol/s)
0.06
0.05
0.04
0.03
0.02
0.01
0
0 100 200 300 400 500
[S] (mM)
0 10
slope 1/[s] 1/[product] 0µl inhibito 0 10 substrate c
100000
6026 ln(x) + 0.054823025273403
80000
1/[Product]
40000
20000
1 1.5 2 2.5 0
0 1 2 3 4 5
[S] (mM)
1/[S]
0.06
= 41974.635317461 x + 46845.4080396346
0.04
V0 (mmol/s)
0.02
f(x) = 0.000447638641795566 ln(x) + 0.01546473
0
0 100 200 300 400 500
= 7990.99441163438
4404.08239750869 x + 18817.7068833186
44313.5479535188
-0.02
1 2 3 4 5 6 7 8
-0.04
18ul Linear (18ul) 6ul
Linear (6ul) 0ul Linear (0ul) -0.06
[S] (mM)
f(x) = 0.000447638641795566 ln(x) + 0.01546473
V0 (
0
0 100 200 300 400 500
= 7990.99441163438
4404.08239750869 x + 18817.7068833186
44313.5479535188
-0.02
1 2 3 4 5 6 7 8
-0.04
18ul Linear (18ul) 6ul
Linear (6ul) 0ul Linear (0ul) -0.06
[S] (mM)
V0 (mmol/s)
0.04
3291036026 ln(x) − 0.0772056037649042
0.02
f(x) = 0.000447638641795568 ln(x) + 0.0181040
0
0 0.5 1 1.5
-0.02
-0.04
-0.06
300 400 500 600 700 800 900 [S] (mM)
[S] (mM)
0 µL Substrate a
0.02
0.018 f(x) = − 0.00049 x + 0.0189
abs/molar=conc slope 1/[s] 1/[product] 0.016
Absorbance (401nm)
0.014
0.012
1.156148E-05 0.01613 7.2727273 86494.118
0.01
1.6245919E-05 0.02317 4.5454545 61553.918 0.008
2.1681175E-05 0.02643 3.030303 46122.961 0.006
4.9961915E-05 0.01005 2.1390374 20015.246 0.004
1.0321001E-05 -0.03673 1.5810277 96889.826 0.002
0.0001095049 0.08219 1.2121212 9132.0117 0
0 2 4
1.1838955E-05 0.01192 0.9569378 84466.912
0.00010601197 0.00482 0.7736944 9432.8971
2.5636561E-05 0.03031 0.6379585 39006.791
1.0870511E-05 0.00501 0.5347594 91991.992
2.2600653E-05 0.02412 0.4545455 44246.509
Absorbance (401
0.1
200 300 400 500 600 700 800 900
0.08
0.06
0.04
0.02
0
[S] (mM) 0 2 4
300 µL Substrate
0.2
0.18
0.16
Absorbance (401nm)
0.14
0.000447638641795568 ln(x) + 0.018104078573995 0.12
0.5 1 1.5 2 2.5 0.1
0.08
0.06
0.04
0.02
[S] (mM) 0
0 2 4
470 µL Substrate
0.3
0.25
Absorbance (401nm)
0.2
0.15
0.1
0.05
0
0 2 4
680 µL Substrate a
0.3
0.25
Absorbance (401nm)
0.2
0.15
0.1
0.05
0.25
Absorbance (401nm)
0.2
0.15
0.1
0.05
0
0 2 4
0 µL Substrate and High Inhibitor (18µL)
0.02
0.018 f(x) = − 0.00049 x + 0.0189
0.016 50 µL Substrate and High Inhibi
0.014
0.06
0.012
0.01
0.05
0.008
0.006
0.04
0.004
0.002
0.03
0
0 2 4 6 8 10 12
0.02
Time (min)
0.01
0
0 2 4 6 8
0.09 0.06
0.08
0.04
0.07
0.06 0.02
0.05
0
0.04 0 2 4 6
0.03
Axis Title
0.02
0.01
0
0 2 4 6 8 10 12
Axis Title
170 µL Substrate and High Inhibitor (18µL) 230 µL Substrate and High Inh
0.14
0.18
0.16 0.12
Absorbance (401nm)
0.14 0.1
0.12
0.08
0.1
0.08 0.06
0.06 0.04
0.04
0.02
0.02
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min) Time (min)
0.12
Absorbance (40
0.08
0.1
0.08 0.06
0.06 0.04
0.04
0.02
0.02
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min) Time (min)
300 µL Substrate and High Inhibitor (18µL) 380 µL Substrate and High Inh
0.2
0.08
0.18
0.16 0.07
0.14 0.06
Absorbance (401nm)
0.12 0.05
0.1
0.04
0.08
0.06 0.03
0.04 0.02
0.02 0.01
0
0 2 4 6 8 10 12 0
0 2 4 6
Time (min)
Time (min)
470 µL Substrate and High Inhibitor (18µL) 570 µL Substrate and High Inh
0.4
0.3
0.35
0.25
0.3
Absorbance (401nm)
0.2 0.25
0.2
0.15
0.15
0.1
0.1
0.05 0.05
0
0 0 2 4 6
0 2 4 6 8 10 12
Time (min)
Time (min)
680 µL Substrate and High Inhibitor (18µL) 800 µL Substrate and High Inhib
0.3
0.3
0.25
0.25
Absorbance (401nm)
0.2
0.2
0.15
0.15
0.1
0.1
0.05
0.05
0.3
0.25
0.25
Absorbance (401nm)
0.2
0.2
0.15
0.15
0.1
0.1
0.05
0.05
0
0 2 4 6 8 10 12 0
0 2 4 6
Time (min)
Time (min)
ubstrate and High Inhibitor (18µL)
4 6 8 10 12
4 6 8 10 12
Axis Title
2 4 6 8 10 12
Time (min)
2 4 6 8 10 12
Time (min)
2 4 6 8 10 12
Time (min)
2 4 6 8 10 12
Time (min)
0.00005
0.00004
0.00003
0.00002
0.00001
0
0 0.0005 0.001 0.0015 0.002 0.0025
Lineweaver-Burk Plot
80000
70000
60000
50000
40000
30000
20000
10000
0
0 1000 2000 3000 4000 5000 6000 7000 8000
slope
0.01328
0.01367
0
0.00686
0.00209
0.00582
0.00681
-0.00087
slope
0.03274 0.04
-0.00916
-0.0069 0.03
-0.01469
0.02
0.01
-0.01
-0.02