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Dna Replication Worksheet

DNA replication is the process by which a cell makes an exact copy of its DNA when it divides. During replication, the DNA double helix unwinds and each strand serves as a template for synthesis of a new complementary strand. This results in two identical DNA molecules, each with one original and one new strand. Specifically: 1. Enzymes break the hydrogen bonds between the DNA bases, unwinding the helix and exposing the single strands. DNA polymerase then adds complementary nucleotides to each single strand, building new double helices using the original strands as templates. 2. This semi-conservative process results in two new DNA molecules that are identical to the original DNA, with one original and one new strand each

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215 views3 pages

Dna Replication Worksheet

DNA replication is the process by which a cell makes an exact copy of its DNA when it divides. During replication, the DNA double helix unwinds and each strand serves as a template for synthesis of a new complementary strand. This results in two identical DNA molecules, each with one original and one new strand. Specifically: 1. Enzymes break the hydrogen bonds between the DNA bases, unwinding the helix and exposing the single strands. DNA polymerase then adds complementary nucleotides to each single strand, building new double helices using the original strands as templates. 2. This semi-conservative process results in two new DNA molecules that are identical to the original DNA, with one original and one new strand each

Uploaded by

Sakina İmanova
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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DNA Replication

Each time a new cell is made, the cell must receive an exact copy of the parent cell DNA. The new cells then receive
the instructions and information needed to function. The process of copying DNA is called replication. Replication
occurs in a unique way – instead of copying a complete new strand of DNA, the process “saves” or conserves one of
the original strand. For this reason, replication is called semi-conservative. When the DNA is ready to copy, the
molecule “unzips” itself and new nucleotides are added to each side.

1. The first step in the process of DNA Replication begins with ________________ breaking the ___________
bonds between the bases and unzipping the helix.

The __________ enzyme begins to unwind the DNA at the ____________________. This is a specific site on
the DNA.

The helicase enzyme moves in both directions from the _________________, forming a ______________. At
either end is a _____________________, a Y-shaped region where the new strands of DNA are elongating.

2. Single stranded _________________ react with the single-stranded regions on the DNA and __________ the
unwound DNA

3. An _________________, called a ___________ constructs an RNA primer, a sequence of about _________


nucleotides, complementary to the parent DNA. (Remember base pairing)

4. ________________ is the major enzyme involved in DNA replication. The enzyme adds
___________________ to the RNA primer.

Energy is required for the bonding to take place. The nucleotides arrive as _________. The DNA bases have
a ___________ sequence. DNA bases arrive with their own __________ as they are bonded by DNA
polymerase III.

DNA polymerase III can only add a nucleotide to the ________ end of a pre-existing chain of nucleotides and
cannot initiate a nucleotide chain. Therefore the new DNA strand grows from ______ to ______.

Parent strands are ___________. Leading strand is replicated from _____ to ______ while the laggin strand
is replicated from _____ to _____

When replication occurs on the laggings strand the DNA polymerase III reaches ____________ it is replaced
by _______________. At this point the RNA is removed and replaced by _______. The enzyme _______
attached to the DNA strand to form ________________ bonds. New primers are made a a new _________
is put into place.
Note the nucleotides are shown as their 3 parts – sugar
(blue), phosphate (pink) and one of the four bases (colour
codes are below). Colour the DNA model on the first page,
then repeat with the replication model on the second page.
Notice that several nucleotides are floating around, they are
waiting to pair up with their match.

The boxed section shows two new strands of DNA. Colour


the old strand (including its base) red and the new strand
(including its base) green.

The two sides of the DNA ladder are held together loosely
by hydrogen bonds. Color the hydrogen bonds gray

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