Multiplex Suspension Arrays

TGF-β3

105
IL-6
M-CSF ICAM-1

TRAIL
(97)
TGF-β1

TNF-α CTACK

MIF

TNF-α

HGF

IL-13

IL-4 TGF β1
IFN-Υ

Bio-Plex Suspension Array System

®

The Leader in Multiplex Immunoassay Analysis

 The Bio-Plex Suspension
Array System
The Bio-Plex system is a flexible,
easy-to-use microplate-based multiplex
immunoassay system. It enables you to
quantitate up to 100 different analytes in
a single sample, giving you the power to
understand complex relationships among
proteins in normal and disease states.
Multiplex analysis with the Bio-Plex system
provides more information with less
sample. The Bio-Plex system comprises
all products for a complete workflow —
from sample prep reagents and diluents
to sophisticated data analysis — for
unsurpassed sensitivity, precision,
and accuracy.

With the Bio-Plex system,

you can multiplex:
■ Immunoassays
■ Enzyme assays
■ Receptor-ligand assays
■ Nucleic acid hybridization assays

From Multiplex Assay to Results in as Little as 3 Hours

BIO-PLEX IMMUNOASSAY WORKFLOW

Bio-Plex assays are designed using microscopic 1. Coupled Beads 2. Samples 3. Detec
beads, each with a different color code A Bio-Plex assay uses a selection of Sample types ranging from serum, urine, Biotin-lab
(spectral address) to permit discrimination of beads with different spectral addresses, milk, and plasma, to cell lysates (prepared are speci
individual assays. Beads are dyed with different each coupled to antibodies against a with a Bio-Plex cell lysis kit) can be used on each t
ratios of two fluorophores and are thus classified different target. with various Bio-Plex assays.
into 100 unique bead regions (xMAP technology).
■ Add de
■
Add coupled beads to wells ■
Add samples to wells containing ■ Incuba
of a 96-well plate coupled beads
■ Wash
■ Wash ■ Incubate 30 min
■ Wash

0 0 0
10 min 40 min 40
12 3 1 3 1 3 1

2 2 2

28

57

99
 A Multitude of Choices Bio-Plex Assay Builder
Bio-Rad offers a continually growing line Design your own custom assays from our
of assays and associated products for selection of hundreds of analytes in four easy
extracellular and intracellular analysis. The steps. With the Assay Builder you can:
assays are relevant to researchers for ■ Get a price quote
everything from cancer to cardiovascular ■ Place your order online
research and available for use with multiple ■ Look up an existing assay by catalog number
species and sample types. For the most Go to www.bio-rad.com/assaybuilder.
up-to-date target information, go to
www.bio-rad.com/ad/plexibility. x-Plex Assay Express Assay

Multiplex Assays in a Variety

of Panel Configurations for
■ Cytokines, chemokines,
and growth factors
We Mix You Mix
■ TGF-b
■ The Th17 pathway Develop Your Own Assay
■ Cell signaling Develop unique bead-based multiplex
■ Cancer assays with Bio-Plex COOH beads.
■ Diabetes Bio-Plex COOH beads are carboxy-coated
■ Isotyping beads ready to be covalently coupled to
■ Acute phase the protein or nucleic acid of your choice.
■ Couple antibodies, antigens, receptors,
ligands, enzymes, or nucleic acids
■ Couple protein (~6–150 kD) with 1 ml of
beads in <5 hr for use in up to 20 microplates
■ The Bio-Plex amine coupling kit is available
for protein and antibody coupling

ction Antibodies 4. Reporter 5. Assay

beled detection antibodies Fluorescently labeled streptavidin In the Bio-Plex array reader, a red ■
Dyed beads are identified
ific for secondary epitopes reporter binds to the biotin-labeled classification laser and a green reporter by their internal fluorescent
target. detection antibodies. laser illuminate individual beads to identify signature (bead region)
etection antibodies to wells ■ Add reporter to wells each bead’s spectral address and associ- ■ Level of target bound to beads
ated reporter signal. is indicated by intensity of
ate 30 min ■ Incubate 10 min
reporter signal
■ Rinse
■ Multiplex data are reported
■ Resuspend in assay buffer simultaneously

0 0
0 min 20 min ~40 min
3 1 3 1

2 2
12 28 57 99
© 2010 Bio-Rad Laboratories, Inc.
Inc. Bulletin 0000
 Instant Effortless
Proficiency Productivity

Experience comprehensive flexibility.

Bio-Plex Manager provides easy-to-use analysis capabilities for any scientific investigation. In addition,
the software provides data acquisition and instrument maintenance capabilities.*

Instrument Maintenance Protein Quantitation

Gene Expression
Data Acquisition Bio-Plex Manager
Genotyping (SNP)

Data Analysis Ratio Metric (Qualitative)

Become an instant expert with automated data optimization and review functions.
Bio-Plex Manager automatically optimizes then visually demonstrates usable assay range. Easy-to-read
reports highlight assay performance issues.*
BIO-PLEX MANAGER SOFTWARE

Over 50% of multiplex assays are run using

Bio-Plex Manager software. Try Bio-Plex Manager
software and find out why.
* Bio-Plex Manager 6.0 or higher
The Power of
Bio-Plex Assays
Bio-Plex multiplex assays deliver more Analysis of 5 phosphoproteins in 12 samples.
Western Blot Bio-Plex System
data in less time with less sample and with
Number of Phosphoproteins 5 5
fewer manual manipulations. The examples Number of Samples 12 12
to the right show a comparison between a Total Data Points 60 60

Bio-Plex assay and conventional methods. Number of Gels or

96-well Plates 5 1
In the top panel, a Bio-Plex phosphoprotein Data Points per Gel or Plate 12 60
5-plex assay is compared with 5 western Total Time Required ~8 hr + one 3 hr + one
overnight incubation overnight incubation
blots. On the bottom panel, a Bio-Plex
Sample Volume 125 µl 25 µl
human cytokine 27-plex assay is compared Dynamic Range Limited total protein 50–900 µg/ml
with 27 ELISA assays.
Analysis of 27 cytokines in 80 samples.

Proven Results Number of Cytokines

ELISA
27
Bio-Plex System
27
The Bio-Plex system is the most widely Number of Samples 80 80
cited multiplex assay platform used Total Data Points 2,160 2,160

for research in many areas including Number of

96-well Plates 27 1
Alzheimer’s and Parkinson’s diseases, Data Points per Plate 80 2,160
diabetes, obesity, cancer, asthma, cystic Total Time Required >60 hr 3 hr
fibrosis, autoimmune disease, viral Sample Volume Serum or plasma, Serum or plasma,
infections, and vaccine development. >1 ml* 12.5 µl
Cell culture supernatant, Cell culture supernatant,
>1 ml* 50 µl

Open Platform Assay Range Serum or plasma,

2–3,000 pg/ml
Serum or plasma,
0.2–3,200 pg/ml
Cell culture supernatant, Cell culture supernatant,
Bio-Plex Pro™ magnetic assays configured 2–3,000 pg/ml 2.0–32,000 pg/ml
on MagPlex beads are compatible with * Based on 50 µl/well of sample

all xMAP life science instrument supplied

by any Luminex partner. Assay cytokines,
chemokines, growth factors, the Th17
pathway, TGF-b, diabetes, cancer, and a
growing list of targets and applications.
Bio-Plex Manager™ software supports
all Luminex 100 and 200 instruments. In
addition, it supports both magnetic and
IL-23
polystyrene beads and can analyze data
from any xMAP platform. Kits are available
to run Bio-Plex Manager on Luminex assay
readers. For more information, contact your
local Bio-Rad sales representative.
Luminex and MagPlex are trademarks of the Luminex
Corporation. The Bio-Plex suspension array system
includes fluorescently labeled microspheres and
instrumentation licensed to Bio-Rad Laboratories, Inc. TGF-β3 IL-1α
by the Luminex Corporation.

TNF-α
IL-6 IL-17

M-CSF
TGF-β1

ICAM-1

TRAIL
 Bio-Rad
Laboratories, Inc.

Life Science Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 31 3689 6600
Canada 905 364 3435 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
Group France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Malaysia 60 3 2117 5260 Mexico 52 555 488 7670
The Netherlands 0318 540666 New Zealand 64 9 415 2280 Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700
Russia 7 495 721 14 04 Singapore 65 6415 3170 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700
Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 Thailand 66 2 6518311 United Kingdom 020 8328 2000

Bulletin 5405 Rev C US/EG 10-1012 0611 Sig 0211