Bot Op P
Bot Op P
Bot Op P
Strategy by Nikhil Thawal (IFOS AIR 46, CSE 2017 AIR 799) and Ajinkya Yele (Deputy CEO, MPSC
2014 rank 26) with inputs from other Botany optional candidates (esp. Dibya Jyoti Parida Sir).
Serious doubts can be addressed to nikhil.thawal@gmail.com.
Choice of Botany: Choose only if you have background of graduation in life sciences. Otherwise it is
difficult to understand basic concepts. Overlap with GS syllabus is limited (mostly gs3).
Advantage of Botany: A person with science background will feel at home and can avoid the
alienation felt preparing other humanities optionals. Scores have generally been consistent.
How to start:
Topic: Explanation, diagrams, examples, references, contributing scientists with Indian contribution,
uses/applications,
PAPER-I
1. Microbiology and Plant Pathology :
Important crop diseases caused by viruses, bacteria, mycoplasma, fungi and nematodes; Modes of
infection and dissemination; Molecular basis of infection and disease resistance/defence; Physiology of
parasitism and control measures. Fungal toxins. Modelling and disease forecasting; Plant quarantine.
Books: Any Graduation level Microbiology Text, specific topics like prions need to be researched, Crop
Diseases (a list of about 20 diseases can be found in any botany text eg Hait prepare diseases in format as per
previous questions, if needed Selective reading of Agrios), Plant quarantine: Prepare India’s mechanisms (asked
in interview)
’15-85, ’14-85, ’13-80, ’12-50
1. Role of microbes in soil nutrient cycling 10 2015
2. Chemical nature of viroids and their transmission in plants 10 2015
3. Role of Fungi in agriculture and medicine 10 2014
4. Role of microbes in Medicine industry 10 2013
5. Various kinds of biofertilizers and their uses 10 2013
6. Lysogenic type of life cycle in viruses 10 2013
7.
8. Describe the disease symptoms, causal organism and the control measures of loose
smut of wheat 15 2015
9. Describe the symptoms, causal organism and the control measures of white rust of
crucifers. 15 2015
10. Describe the causal organism, disease symptoms and the disease control measures of
Red rot of Sugarcane and Late blight of Potato 12.5+12.5 2015
11. Mycotoxins 10 2015
12. Plant Quaratine 10 2014 12 2012
13. Discuss about the Physiology of parasitism 15 2014
14. Describe the causal organism, disease symptoms and the disease control measures of
Tikka disease of Groundnut, Citrus canker, Rust of Wheat, Ergot of rye 12.5 X 4 2014
15. Describe the causal organism, disease symptoms and the disease control measures of
Wilt of Arhar, Green Ear disease of Bajra, Tundu disease of Wheat, Red rot of
Sugarcane 12.5X4 2013
16. Fungal Toxins 12 2012
17. Differentiate between Prions and Viriods; Rust and Smut diseases;; Downy Mildews
and Powdery Mildews 3X 12 2012
18. Citing suitable examples, explain What are prions and why is there a need to
understand them thoroughly? 20 2011
19. Citing suitable examples, explain How are moulds both a bane and boon for human
beings? 20 2011
20. What is meant by modelling and how does it help in disease forecasting? 15 2011
21. Citing suitable examples, explain how laxity in quarantine procedure can have
disastrous effects. 15 2011
22. Describe the process through which the bacterial genes are transferred from one
bacterium to another by the viruses. 15 2010
23. Iron Bacteria 20 2009
24. What is the importance of Bacillus thuringiensis in the current efforts to raise crop
productivity? 20 2010
25. With suitable examples, briefly describe the microbial processes occurring in the
rhizosphere which help in plant growth. 25 2010
26. Five different types of spores are produced by the rust fungi. Mention their names
and their salient points of distinction from each other. 15 2010
27. Microbes in the control of water pollution. 2009
28. Describe the types of plant diseases caused by viruses. Explain their control measures.
20 2007
29. Ultrastructure of a bacterial cell 20 2007
30. Asexual reproduction in Aspergillns 20 2007
31. Prions 2007
32. Molecular basis of infection 20 2006
33. Microbes as indicators of pollution 20 2006
34. DWLD Lytic cycle 20 2006
35. DWLD Diagnostic stages of any two powdery mildews 20 2006
36. What are the symptoms and causes of hay fever? 20 2006
37. Differentiate between conjugation and transduction in Bacteria. 20 2005
38. Write in short about mycotoxins. 20 2005
39. Description of, conidia formation in Aspergillus. 20 2005
40. Differentiate between bacteria and cyanobacteria. 20 2004
41. Give an account of algae in industry. 20 2004
42. Discuss the sequential steps involved in host pathogen interaction. 20 2004
43. Mention the causal organism, symptoms and control measures of bacterial blight in paddy,
sandal spike and leaf spot in groundnut. 30 2004
44. Give the role of bacteria in fermentation and mention the products. 30 2004
45. Describe the lysogenic life cycle in viruses. 20 2003
46. Describe various types of Path toxins and their role in plant diseases. 20 2003
47. With the help of suitable describe the life cycle of Aspergilius and discuss the economic
importance of this genus. 60
48. Ergot of Bajra 20 2003
49. Blast of Rice 20 2003
50. Stem gall of coriander 20 203
2. Cryptogams :
Algae, fungi, lichens, bryophytes, pteridophytes structure and reproduction from evolutionary
viewpoint; Distribution of Cryptogams in India and their ecological and economic importance.
Books: Text book of Botany-Singh, Pandey, Jain/ by Bhattacharya, Hait and Ghosh
3. Phanerogams :
Books: Angiosperms by BP Pandey (use any available, Hait Bhattacharya is sufficient
anyway), Taxonomy notes must be up to date
Angiosperms: Systematics - Gurcharan Singh(prepare the intial 4 chapters which contains icbn,alpha
omega taxonomy, nomenclature rules)
1. Write an account of the following families with respect to floral structures. Give the
botanical names of three important plant species belonging to each and specify their
uses: Ranunculaceae and Fabaceae 10+10 2015 ; Orchidaceae, Magnoliaceae,
Asteraceae, Rubiaceae 12.5 X4 2014; Poaceae, Liliaceae, Asclepiadaceae 17+17+16
2013
2. Distinguish the Androecium of Malvaceae, Asteraceae and Asclepiadaceae from each
other 12.5 2015
3. Compare the floral characters of the family Solanaceae and Malvaceae 12.5 2015
4. DNWD TS of cyathium of Euphorbia and VS of leaf of Welwitschia 12.5 X2 2014
5. Make a comparision of Androecium in Malvaceae, Asteraceae and Cucurbitaceae 12
2012
6. Asteraceae is considered as one of the highly evolved families of angiosperms. Give
an account of advanced characters in Asteraceae 15 2012
7. Compare and contrast the floral characters of the three sub-families of Leguminosae
15 2012
8. Discuss the role of amphibious plants in the evolution of land plants. 15 2011
9. What are the differences between Apiaceae and Umbelliferae. 10 2011
10. What are the differences between the Palaeozoic and Coenozoic plants 10 2011
11. Describe Gynoecium in Asclepiadaceae, Brassicacea and Poaceae. 10 2011
12. Discuss whether the angiosperms are monophyletic or polyphyletic.20 2010
13. With suitable examples, enumerate the floral characteristics of bird pollinated
trees. 20 2010
14. Compare the androecium in the following groups of families : (i) Malvaceae and
Fabaceae (ii) Asclepiadaceae and Asteraceae (iii) Solanaceae and Brassicaceae 20
2010
15. With the help of well-labelled diagrams, explain the distinguishing features of Flower
of Dolichos and Caesalpinia. 20 2009
16. Give an account of the use of pollen morphological characters in systematics. 20 2007
17. Compare the outline of system of classification of angiosperms proposed by
Hutchinson with the system proposed by Bentham and Hooker. 20 2007
18. Draw neat and labelled diagrams of the Stomata of sunflower and maize 20 2007
19. Give an account of diagnostic characters of Liliaceae and Palmae with suitable
20. examples. 30 2007
21. Describe the morphological nature of commercial cotton, clove and saffron. 20 2006
22. Describe the phyletic position of Magnoliaceae. 20 2006
23. How will you distinguish between the following pairs of families on the basis of
24. important floral characters?
a) Malvaceae and Brassicaceae b) Leguminosae and Solanaceae
25. Floral structure and primitive characters of Magnoliaceae. 20 2004
26. Floral structure and floral formula of Poaceae. 20 2004
27. Differentiate between the pollinia of Asclepiadaceae and Orchidaceae. 20 2004
28. Give the salient features of Bentham and Hooker's system of classification. 20 2004
29. Write an account of floral structures of the following families. Give botanical names
of three important plant species belonging to each of the three families and specify
their uses a) Astaracae, b) Solanacae, c) Asclepiadaceae
Stomata and their types; Glandular and non-glandular trichomes; Unusual secondary growth; Anatomy
of C3 and C4 plants; Xylem and phloem differentiation; Wood anatomy.
Domestication and introduction of plants; Origin of cultivated plants, Vavilov’s centres of origin.
Plants as sources for food, fodder, fibres, spices, beverages, edible oils, drugs, narcotics, insecticides, timber,
gums, resins and dyes; latex, cellulose, starch and its products; Perfumery; Importance of Ethnobotany in Indian
context; Energy plantations; Botanical Gardens and Herbaria.
5. Morphogenesis :
Totipotency, polarity, symmetry and differentiation; Cell, tissue, organ and protoplast culture. Somatic
hybrids and Cybrids; Micropropagation; Somaclonal variation and its applications; Pollen haploids, embryo
rescue methods and their applications.
Books: tissue culture by razdan, plant development chapter in Gilbert developmental biology book,
Plant Cell and Tissue Culture by Indra Vasil, Also quote Indian contributions esp. Guha and Maheshwari in
answers, Very scoring!
PAPER-II
1. Cell Biology :
Techniques of cell biology. Prokaryotic and eukaryotic cells—structural and ultrastructural details;
Structure and function of extracellular matrix (cell wall) and membranes-cell adhesion, membrane transport and
vesicular transport; Structure and function of cell organelles (chloroplasts, mitochondria, ER, dictyosomes
ribosomes, endosomes, lysosomes, peroxisomes; Cytoskeleton and microtubules; Nucleus, nucleolus, nuclear
pore complex; Chromatin and nucleosome; Cell signalling and cell receptors; Signal transduction Mitosis and
meiosis; molecular basis of cell cycle. Numerical and structural variations in chromosomes and their
significance; Chromatin organization and packaging
of genome; Polytene chromosomes; B chromosomes— structure, behaviour and significance.
35-’15,
Book: verma agarwal, plus p k gupta genetics book. I used World of cell (I had used in Institute, not
recommended for others), you can refer to any cell biology book, Prepare notes specific to plant cell organelle
characteristics!
1. Polyteny and Polypoidy-differentiate
2. Mitochondria 10 2015
3. Extrisic and Intrinsic proteins 10 2015
4. Diff bet pro and Eukaryotic cells 15 2015
5. Cell cycle. Imp. Proteins that control it 20 2014
6. Structure and composition of Ribosome 15 2014
7. Exposing Polytene chromosomes to Actinomycin 10 2013
8. Removal of H1 protein from cell 10 2013
9. Exposing dividing cells to CDK1 10 2013
10. Formation, structure and chemical composition of Glycocalyx 20 2013
11. Pachytene 10 2013
12. Most abundant Biological molecule in plants and its types 15 2013
13. Pathways taken by secreted proteins 10 2012
14. Compare different types of RNA and protein components found in Ribosomes of
prokaryotes and eukaryotes 20 2012
15. Role of CdK and cyclins 15 2012
16. Explain how the chromatin modification leads to genome expression. 15 2011
17. What are the major functions of cytoskeleton in living cells? 15 2011
18. Describe briefly, how RNA molecule transports within the eukaryotic cell. 15 2011
19. Signal transduction in Prokaryotes 15 2010
20. Explain Origin of mitochondria 15 2010
21. Distinguish between Smooth and rough ER 10 2010
22. Distinguish between Ribosomes and Peroxisomes 10 2010
23. Distinguish between Membrane proteins in ion transport. 10 2010
24. Short notes on Organelle genomes 20 2009
25. Distinguish between channels and carrier proteins and their roles in regulating
membrane transport. 30 2009
26. Distinguish between Heterochromatin and Euchrornatin 30 2009
27. Structure of mitochondria in relation to function 20 2007
28. Describe the molecular basis of cell cycle 30 2007
29. Explain the structure and significance of lampbrush and B-chromosomes 30 2007
30. Structure of plasma membrane 20 2006
31. Polytene Chromosome 20 2005
32. Signal Transduction 20 2005
33. Describe in detail the ultrastructure of Golgi Complex and its function. 20 2005
34. Meiosis 20 2004
35. Describe in detail the structure and functions of mitochondria (60). 2004
36. Structure and Significance of Lamp brush Chromosomes. 20 2003
37. Describe in detail the physical properties of cell membrane. 30 2003
38. How does the water and solutes move through the cell membrane? 30 2003
Development of genetics, and gene versus allele concepts (Pseudoalleles); Quantitative genetics and
multiple factors; Incomplete dominance, polygenic inheritance, multiple alleles; Linkage and crossing over of
gene mapping including molecular maps (idea of mapping, function); Sex chromosomes and sexlinked
inheritance; sex determination and molecular basis of sex differentiation; Mutations (biochemical and molecular
basis); Cytoplasmic inheritance and cytoplasmic genes (including genetics of male sterility).
Structure and synthesis of nucleic acids and proteins; Genetic code and regulation of gene expression;
Gene silencing; Multigene families; Organic evolution-evidences, mechanism and theories. Role of RNA in
origin and evolution.
’15-80,
Books: It is our observation that some obscure questions keep coming from books written by PK Gupta
1. Describe about the factors that affect the recombination frequencies 10 2016
2. C-value paradox 10 2016
3. Chemical Mutagens 10 2016
4. “The consequences of translocations are profound. They lead not only to a change in gene
linkage in the translocated sections but also to easily incurred meiotic abnormalities.”
Discuss. 15 2016
5. Citing suitable evidences, explain plastid inheritance in four o’clock plant. 15 2016
6. Genetic Code 10 2015
7. Evidence of organic evolution 15 2015
8. Structure behaviour and significance of special type of chromosomes 15 2015
9. Role of RNA in origin and evolution 20 2015
10. Mutations (biochemical and molecular basis) 20 2015
11. Mendels law of Inheritence [dominance] differ from incomplete dominance 10 2014
12. Why do chromosomal aberrations happen 15 2014 describe various chromosomal
aberrations 20 2015
13. Glucose grown cells of E. coli transferred to lactose medium, fate of Lac operon 15
2014
14. rRNA for phylogenetics study 15 2014
15. exp. Evidence of DNA as genetic material 15 2014 10 2012
39. Illegitimate crossing over between normal and accessory chromosome 10 2013
40. Intra-allelic recombination 10 2013
41. With example, explain tripartite type of sex determination 15 2013
42. ‘Genes donot arise denovo’. Keeping in view this fact describing the phenomena that
must have helped in increasing the no. of genes during evolution 20 2013
43. CMS gene location and action, what are Restorer genes 15 2013
44. Neo-Darwinism 10 2013
45. Linkage 10 2013
46. Role of RNA world in origin and evolution 10 2012
47. Dist bet suppressor and reverse mutation 10 2012
48. Site specific recombination and role of recombinase enzyme 10 2012
49. Annueploidy and chromosomal variations found in aannuploid plant 20 2012
50. Transposoms and how do they cause mutations 20 2012
51. Lac operon and its expression in presence and absence of functional regulatory gene
15 2012
52. Chromosomal basis of sex differentiation in plants 15 2012
53. Infectious proteins brought a change in concept of inheritance 15 2012
54. Fate of two homologous chromosomes that have undergone unilateral crossing over
15 2012
55. Describe about the factors that affect the recombination frequencies. 15 2011
56. Explain antisense RNA technology with an example. Highlight its applications. 15
2011
57. How can you identify the multigene families? Discuss the significance of identical or
non-identical genes of multigene families. 15 2011
58. Explain the Theory of Age and Area. 15 2011
59. Discuss the mechanism of the regulation of gene activity in lactose operon "with"
reference to beta-galactosidase activity. 15 2011
60. Distinguish between monoploid and haploid and their role in evolution. 15 2011
61. What is RNA splicing? Describe the known mechanism of RNA splicing for group-
II introns. 15 2011
62. How can you prove that DNA is a genetic material? Describe with a suitable
example. 15 2011
63. Explain Spontaneous mutations 15 2010
64. Explain RFLPs 15 2010
65. Differentiate between : Negative and positive gene regulation. 15 2010
66. Differentiate between Homologous and Heterologous recombinations 15 2010
67. Differentiate between Exons and Introns. 15 2010
68. Differentiate between Transition and Transversion. 15 2010
69. Tautomeric shifts and mutations 20 2009
70. Bidirectional synthesis of DNA in E. Coli 20 2009
71. Comment briefly on the contributionsmade by Herman Nilsson-Ehle. 15 2009
72. Male sterility 20 2007
73. Describe the principles of protein biosynthesis. 2007
74. Discuss the methods of gene mapping 30 2007
75. Polytene chromosomes 20 2006
76. DNA damage and repair 20 2006
77. Explain linkage and crossing-over. Describe the methods of construction of gene
78. Maps. 30 2006
79. What are mutations? Describe different types of mutations and add a note on chemical
mutagens 30 2006
80. Uses and applications of correlation and regression analysis in plant genetics 20 2006
81. Explain the mechanism of DNA replication 20 2006
82. Discuss cytoplasmic inheritance 20 2006
83. Describe in brief the regulation of gene expression in eukaryotes 20 2006
84. Describe properties of Genetic Code. 20 2005
85. Describe the role of RNA in origin and evolution. 60 2005
86. Sex-linked inheritance 20 2004
87. Discuss the synthesis of nucleic acids and proteins (60). 2004
88. Evolution. 20 2003
89. Gametophytic apomix. 20 2003
90. What is Cytoplasmic inheritance? 10 2003
91. Describe the Cytoplasmic-genetic male sterility. 20 2003
92. Describe in detail the method of producing hybrid seeds using Cytoplasmic-genetic
male sterility system. 30
Books: Directly referred to Net resources and Molecular biology Text by Watson. Biostatistics is not to be
ignored, keeps coming in compulsory questions.
1. Molecular markers 10 2016
2. Hybrid Vigour 10 2015
3. Self incompatability 10 2015
4. Methods of Gene Transfer 20 2015
5. Method of developing disease and insect resistance varieties by Back-cross method 15
2015
6. Numerical variations in chromosomes 15 2015
7. Mechanism of disease resistance 15 2015
8. Selection of Cytoplasmic genetic male sterility in the development of hybrid variety
10 2014
9. FISH in tracking the target organism 10 2014
10. Application of radio memetic chemicals 10 2014
11. Test of significance in biology 10 2014
12. What are molecular probes, mention application in biology 15 2014
13. How would you develop improved variety of basmati rice for organic farming
conditions 20 2014
14. GISH in indentifying putative ancestors of plants 15 2013
15. With the help of well established example explain how replacement of a purine by a
pyrimidine can induce mutation 15 2013
16. Balanced Lethal System 10 2013
17. Chi-square test 10 2013
18. DNA-DNA Hybridisation in study of biodiversity 15 2012
19. Gene markers and imp in biological studies 15 2012
20. Use of apomixes in plant breeding 15 2012
21. Primers and PCR 15 2012
22. Concept of blotting and Southern blotting 15 2012
23. Discuss the mechanism of T-DNA transfer from Agrobacterium tumefaciens to host
plant. 15 2011
24. Comment on 'EPSPS' and 'Tra' genes 15 2011
25. Describe different methods of gene transfer in plants. 15 2011
26. Explain interspecific and intergeneric hybridization. Comment on Raphano-brassica.
15 2011
27. Highlight the biosafety aspects arising out of plant genetic engineering. 15 2011
28. Explain the current developments in transgenic technology contributing to crop
improvement. 15 2011
29. How will you identify the expression of a cloned gene? 15 2011
30. Define agamospermy. What is its significance in crop improvement? 20 2010
31. Explain Normal distribution. 15 2010
32. Explain FISH technique and its importance in understanding genomic relationships
among spec1es. 30 2010
33. Describe in detail the Agrobacterium-mediated gene transfers in the development of
transgenic crop pants. 30 2010
34. Describe the method of developing maize hybrids. 30 2010
35. Backcross method of breeding 20 2009
36. Discuss the origin. production and uses of haploids. 30 2009
37. Describe biosafety regulatory measures for raising transgenic crops. 30 2009
38. List the important statistical methods of studying association and relationship
between two variables. 15 2009
39. Give an account of the PCR based molecular markers and their applications in plant
breeding. 30 2009
40. Probability and distributions, with suitable illustrations. 20 2007
41. Explain the methods of transfer of genes into plants 30 2007
42. Describe the methods of selection and hybridization used in plant breeding 30 2007
43. Molecular markers in plant breeding 20 2006
44. Techniques of breeding self-pollinated crops 20 2006
45. Transgenic plants. 20 2005
46. Standard deviation. 20 2005
47. Define hybrid vigour. Describe anyone method of hybridization for crop
improvement. 60 2005
48. Mass selection 20 2004
49. Chi-squre test 20 2004
50. Describe the back-cross method of breeding in crop varieties (30). 2004
51. Describe in detail the various uses of back-cross method of breeding and its
limitations (30). 2004
52. Pure Line Selection. 20 2003
53. What do you understand by genetic engineering? 15 2003
54. Describe in detail the agro bacterium-mediated gene transfer method for producing
transgenic plants. 45 2003
Water relations, mineral nutrition and ion transport, mineral deficiencies. Mechanism of phloem
transport, Importance of secondary metabolites. Pigments as photoreceptors (plastidial pigments and
phytochrome).
Plant movements; Photoperiodism and flowering, vernalization, senescence; Growth substances—their
chemical nature, role and applications in agri horticulture; growth indices, growth movements.
Stress physiology (heat, water, salinity, metal); Fruit and seed physiology. Dormancy, storage and
germination of seed. Fruit ripening—its molecular basis and manipulation.
Concept of ecosystem; Ecological factors. Concepts and dynamics of community; Plant succession.
Concepts of biosphere; Ecosystems; Conservation; Pollution and its control (including phytoremediation); Plant
indicators; Environment (Protection) Act.
Forest types of India—‘Ecological and economic importance of forests, afforestation, deforestation and
social forestry; Endangered plants, endemism IUCN categories, Red Data Books; Biodiversity and its
conservation; Protected Area Network; Convention of Biological Diversity, Farmers’ Rights; and Intellectual
Property Rights; Concept of Sustainable Development; Biogeochemical cycles. Global warming and climatic
change; Invasive species; Environmental Impact Assessment; Phytogeographical regions of India.