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Exercise 12

This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

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Bhumika Mahalik
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148 views2 pages

Exercise 12

This document provides instructions for performing controlled pollination through emasculation. It describes the process as: (1) Selecting a flower bud before anthesis and removing the stamens to produce a female parent plant. (2) Covering the emasculated flower with a bag to protect it from unwanted pollen during bagging. (3) Transferring pollen from the desired male parent plant directly onto the stigma of the emasculated female flower. The goal is to control which parental plants are used to produce seeds with desired traits through human manipulation of the reproductive process.

Uploaded by

Bhumika Mahalik
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Exercise 12














Procedure
(i) Select a flower in bud condition where antheses has not occurred. Open
the bud carefully and remove the stamens (Fig. 12.1). Mark this as
female parent plant.

Fig. 12.1 Showing process of Emasculation

21/04/2018
LABORATORY MANUAL: BIOLOGY

(ii) Cover the emasculated flower with a plastic


bag to protect it from undesired pollen
(Bagging) (Fig. 12.2). The bag should be
held securely in place with a paper clip/
string/thread. Select the size of bag in
accordance with the flower size. Bags
must be transparent with minute pores.

Fig. 12.2 Bagging of an emasculated flower

(iii) Bring into physical contact anthers of a


desired male plant containing mature
pollen grains with the stigmatic surface
of emasculated female flower (Fig. 12.3).
Use tweezers/brush if necessary to dust
the stigmatic surface with pollen.

(iv) Cover the pollinated flower again with the


bag immediately. For identification, label
the female parent (Tagging). Each
pollinated flower should bear a label
Fig. 12.3 Showing cross pollination on an containing the name of the seed parent,
emasculated flower the letter X (to signify a cross), the name
of the pollen parent, and the date on which
the cross was effected.

Questions
1. Why is emasculation performed before anthesis?
2. What are the advantages of using a bag containing minute pores?

46

21/04/2018

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