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    Pre-Lab Bacterial Isolation

    Uploaded by

    Helen
    The document provides instructions for a pre-lab assignment on bacterial isolation. It includes links to two videos demonstrating streak plate and spread plate techniques. It asks students to define a pure culture and give examples of when a pure culture is required. The purpose of streak plating is to isolate pure cultures by allowing single microorganisms to grow into separate colonies. Figure 3 shows results of spread plating with varying colony numbers, indicating different initial concentrations of microbes in each specimen.

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    Pre-Lab Bacterial Isolation

    Uploaded by

    Helen
    19 views2 pages
    The document provides instructions for a pre-lab assignment on bacterial isolation. It includes links to two videos demonstrating streak plate and spread plate techniques. It asks students to define a pure culture and give examples of when a pure culture is required. The purpose of streak plating is to isolate pure cultures by allowing single microorganisms to grow into separate colonies. Figure 3 shows results of spread plating with varying colony numbers, indicating different initial concentrations of microbes in each specimen.

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    The document provides instructions for a pre-lab assignment on bacterial isolation. It includes links to two videos demonstrating streak plate and spread plate techniques. It asks students to define a pure culture and give examples of when a pure culture is required. The purpose of streak plating is to isolate pure cultures by allowing single microorganisms to grow into separate colonies. Figure 3 shows results of spread plating with varying colony numbers, indicating different initial concentrations of microbes in each specimen.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    19 views2 pages

    Pre-Lab Bacterial Isolation

    Uploaded by

    Helen
    The document provides instructions for a pre-lab assignment on bacterial isolation. It includes links to two videos demonstrating streak plate and spread plate techniques. It asks students to define a pure culture and give examples of when a pure culture is required. The purpose of streak plating is to isolate pure cultures by allowing single microorganisms to grow into separate colonies. Figure 3 shows results of spread plating with varying colony numbers, indicating different initial concentrations of microbes in each specimen.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Pre-lab for Bacterial Isolation Lab (10 points)

    View the following 2 videos, read the lab on Bacterial isolation and answer the questions. Submit you
    answers on Bb.

    Preparing a streak plate:


    https://stevensonuniversity-my.sharepoint.com/:v:/g/personal/syoungren_stevenson_edu/
    ESZZ2ZzsCfFAlEwwHeYHuiQBFPhp4opk-23DJdSKRfeZfA?e=AysvCp

    Preparing a spread plate:


    https://stevensonuniversity-my.sharepoint.com/:v:/g/personal/syoungren_stevenson_edu/
    EWH99e7SEDhCsHToCIB526gBAPcqW0cU5WFH_pNkqvZntA?e=R1EBy8

    1. Define a pure culture and give at least 2 examples when a pure culture of bacteria are required.

    A pure culture is a group of cells that’re all the same. They could have come from one parent cell, a
    group of related cells, or just any relation that makes them all similar. In terms of bacteria, it means
    that only one type of bacterial cell is being produced or growing in an area (1 organism growing in an
    area)
    Two examples of when a pure culture of bacteria is required is when:

    1. Needed to research and determine the cause for a bacterial infectious disease. When medical
    professionals are faced with an unknown disease, they tend to take a clinical sample to analyze,
    which’ll end up showing the microbes which are normal and those that’re the cause of the
    disease. With pure culture(s), they’re able to separate the bacteria and analyze it as it grows. By
    observing only the bacteria’s infectious manners (without other organisms there), scientists will
    ultimately understand the bacteria and be led to a solution.

    2. Pure culture can be used and put through tests to understand and study specific species and how
    they survive and/ or adapt to certain conditions.

    2. What is the purpose of preparing a streak plate? A successful streak plate technique will result in a
    number of isolated colonies. How do these colonies arise?

    The purpose of preparing a streak plate is to isolate pure cultures. It’s mostly used to get colonies of
    bacteria isolated from a mixed population so it can be identified. The way colonies arise is through one
    singular micro-organism.
    3. In the lab handout, Figure 3 illustrates the results of a spread plate technique. In a few sentences
    explain why we see varying numbers of colonies on the 5 plates shown.

    The fact that each plate has a different number of microbes in one specimen accounts for the variations in
    numbers we observe on the five distinct plates. In plate 1, you can see that there are many more colonies
    than in the other plates. This shows that plate 1 has more different bacteria than the other plates. Now
    comparing the plates to plate number 5, We can say that plate 5 displays the fewest colonies indicating
    the least number of different microbes.

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