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DNA Replication Transcription and Translation

The document provides instructions for an assignment on DNA transcription and translation. Students are asked to write out complementary base pairs, describe DNA nucleotides, transcribe a DNA sequence to mRNA and translate the mRNA to amino acids. The assignment further involves creating DNA, mRNA, and amino acid sequences, as well as describing the stages of transcription including capping, splicing, and polyadenylation of mRNA.

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Toby Trolly
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198 views2 pages

DNA Replication Transcription and Translation

The document provides instructions for an assignment on DNA transcription and translation. Students are asked to write out complementary base pairs, describe DNA nucleotides, transcribe a DNA sequence to mRNA and translate the mRNA to amino acids. The assignment further involves creating DNA, mRNA, and amino acid sequences, as well as describing the stages of transcription including capping, splicing, and polyadenylation of mRNA.

Uploaded by

Toby Trolly
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Assignment:

For all: Do as directed. Write your answers in A4 size paper with 1 inch margin on all sides.
Write your name and section on the left side of the paper. See the instruction for submission
below.

1. Write down the names of the bases that could pair with each to make a complementary
strand.

Cytosine Guanine Adenine Thymine

Guanine Cytosine Thymine Adenine

2. What are the components of a DNA nucleotide? How do these connect together? A DNA
nucleotide is composed of a deoxyribose sugar, a nucleobase, and a phosphate group. Within
each strand, covalent bonds hold these nucleotides together. One nucleotide's sugar makes a
covalent bond with another's phosphate group.

3. Your first task is to create a strand of DNA with 15 nucleotides which you will need to
translate into a protein. Provide the 15 nucleotide long strand of DNA on lines below. Note:
Always indicate the 5’ and 3’ ends.

5’ C G A T G C A T T A C G T A C 3’
3’ G C T A C G T A A T G C A T G 5’

Transcribe the DNA strand above into mRNA strand.

5’ C G A U G C A U U A C G U A C 3’

Your next task is to translate the mRNA into amino acids. Use the genetic code in Fig. 11 to
do this.

Arg – Cys – Ile – Thr – Tyr

4. A. Another task is to creator an amino acid chain that is 6 amino acids long. Convert the
amino acid chain into an mRNA chain. Your last task will be to convert the mRNA chain into
a DNA chain. You then need to provide the complementary strand for the DNA.

Amino Acid: Lys Leu Pro Glu Val Asn


mRNA chain: 5’ A A G U U G C C C G A G G U A A A C 3’
DNA chain: 3’ T T C A A C G G G C T C C A T T T G 5’
5’ A A G T T G C C C G A G G T A A A C 3’
A. A strand of DNA has the following sequence of bases.
5’ GTACTACATGCTATGCAT 3’
Fill in the blanks to show the base sequence for the messenger RNA sequence formed from the
DNA during transcription.

5’ C A U G A U G U A C G A U A C G U A 3’
A. To the mRNA strand above, add cap: 5’ GPPP CAUGAUGUACGAUACGUA 3’
B. Remove the introns “UGUA” and “AUAC”: 5’ GPPP CAUGACGGUA 3’
C. Add a poly A tail onto the 3’ end: 5’ GPPP CAUGACGGUA AAA∙∙AA 3’

5. Why do you need to know how does translation relate to your life? We need to know how
translation relates to our life so that we are aware why and how things work the way they do.
It also important for us to have knowledge on what is going on with our lives.

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