AES2016 Annual Report Black Soldier Flies
AES2016 Annual Report Black Soldier Flies
AES2016 Annual Report Black Soldier Flies
net/publication/311847451
UVI/AES Annual Report 2016 - Alternative Sources of Food for Aquaponics in the
U.S. Virgin Islands: A Case Study with Black Soldier Flies
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Alternative Sources of Food for Aquaponics in the U.S. Virgin
Islands: A Case Study with Black Soldier Flies
By Lorenzo Cannella, Abdel Rahman Ahmed Nassef, Donald Bailey and Rhuanito S. Ferrarezi
Overview of black soldier fly growing unit. Left: Abdel Rahman Ahmed Nassef, Right: Lorenzo Cannella.
in total weight gain compared to regular feed based daily requirements of fish feed. Then we would feed
on 40% protein (67% fish source, 33% plant source) the fish in the CAS with BSF in order to be more
(St-Hilaire et al., 2007). This has caused a reduction sustainable and reduce the costs of fish feed.
of 38% in oil fish component, the unsustainable
component of fish feed, but at the same time a MATERIAL AND METHODS
decrease of oil fish component due to a decrease of The project started by adapting project designs
omega 3 fatty acids in fish muscle (St-Hilaire et al., available for hobbyists to one that can have the
2007). The replacement of 10% fishmeal by 10% possibility to produce all BSF phases (larvae, pre-pupa
BSF larvae did not reduce the growth rate of catfish and adult stage). Our setup also provides space for
cultivated in culture tanks (Bondari and Sheppard, reproduction, keeping the flies inside the growing unit.
1987). Black soldier flies have a chitin shell, which is
indigestible to vertebrate animals and can clog filters Black soldier fly growing unit
and pumps. When BSF are dried and mixed with The BSF growing unit has a dimension of 2.5 ×
other ingredients its use is similar to the commercial 1.8 × 1.2 m (Figure 2). The system was assembled
feed (St-Hilaire et al., 2007; Bondari and Sheppard, using two 2.5 × 0.6 m and two 1.2 × 0.6 m plywood
1987). Feeding the fish with 100% larvae did not sheets. Plywood was also used to assemble two sliding
provide sufficient dry matter or protein intake for doors for the larvae collection compartment and the
good growth (St-Hilaire et al., 2007). Positive results 2.5 × 1.2 m roof. The dimensions of the growing
have been collected when fish food was substituted unit were 0.9 × 1.2 × 0.6 m, with a volume of 0.648
with BSF pre-pupae as 25% and 50% replacement m3. The BSF growing unit was surrounded by a 1
for the fish meal component of the control diet (St- × 5 m plastic screen (2 × 2 mm mesh) to cover the
Hilaire et al., 2007). Furthermore, Stamer et al. (2014) sides, keep the flies inside and allow air movement.
have indicated that substituting 50% of rainbow trout In the front there was a 0.9 × 0.9 m door made with
fishmeal with BSF decreased the feed conversion plywood to allow feeding activities inside the growing
ratio and protein retention ratio, resulting in increased unit (Figure 2). The project design was created using
body weight gain. Based on Stamer et al. (2014) and Google SketchUp 2016 (Google, Mountain View,
St-Hilaire et al. (2007), the percentage of BSF pre- CA).
pupa in the fish feed should between 25% and 50% Inside the growing unit there are two ramps with
(and never exceed 50%) of the total commercial feed a hole on the top to help the BSF larvae move from
amount or other component, to stimulate a significant the growing media to a 26-L tray (Sterilite, Fitchburg,
gain of weight. MA) installed in both sides (Figure 2). During the pre-
The goal of this project is to provide guidelines pupa stage, larvae start migrating from the food area
related to designing and assembling a BSF growing to the 26-L trays. The larvae start a metamorphosis
unit and produce enough BSF pre-pupa to supply the phase, where they stop eating and empty their
36
trial we added ground coffee in the media.
Table1.1.Different
Table Differentdiet
dietcomposition
compositionduring
duringthe
thetrial.
trial.
Vegetable Cafeteria Water added Frequency per
Day waste Fish waste (L) week
3 to 15 ✓ 3 2
16 to 28 ✓ ✓ ✓ 3 2
29 to 71 ✓ 7 2
stomach.
Procedures
For this reason, the BSF move into a new maintain the media moisturized by using a 3.78-L
area without food and water. Our growing unit design sprayer (Root-Lowell manufacturing Co., Lowell, MI).
Both sides of the growing unit were
is adequate to maximize the BSF migration and checked daily and the
WeBSFaddedpre-pupae
1.5 to 2 were
kg of collected. If
tilapia carcass every 3 days to
another type of fly was found inside the system, it was manually removed. The pre-pupae
simplify the harvest by having the larvae BSF fall into feed the BSF. We recorded the amount and weight of
collected were counted and weighed and returned to a 3.78-L bucket (Sterilite, Fitchburg, MA)
the 26-L trays. larvae, water, feed and coffee during the trial.
inside the growing unit to allow metamorphosis into the adult phase and generation of more
ToWe
flies. facilitate the reproduction
also added 7 L of waterofdaily
BSF toadults, we the media moisturized by using a 3.78-L
maintain
attached a piece of corrugated cardboard
sprayer (Root-Lowell manufacturing Co., Lowell, MI). (25 × 25 cm)We Temperature
added 1.5 to 2monitoring
kg of tilapia carcass
on the wall of the growing unit. The BSF spawn the
every 3 days to feed the BSF. We recorded the amount and weight An automated
of larvae,system
water, was
feedused
and to monitor
eggs
coffeein during the trial. and the larvae fall down directly environmental conditions inside the growing unit
the cardboard
onto the compost after hatching. Our BSF growing from day 77. We used a low-cost open-source
unit has a cost of approximately $250. microcontroller (Arduino Mega 2560 R3; Arduino,
The larvae monitoring
Temperature were obtained from a commercial Ivrea, Italy) connected to a compost temperature
An automated system was used to monitor environmentalsensor
supplier available at Amazon.com (Alfredo Llecha, (DS18S20;
conditions inside Adafruit,
the growingNewunit
York, NY) and an air
GA)
fromanddaydelivered
77. We used in aasmall container
low-cost with coffee
open-source microcontrollertemperature and relative
(Arduino Mega 2560 R3; humidity
Arduino,sensor (AM2302;
grounds to keep
Ivrea, Italy) the larvae
connected to aalive.
compostThe temperature
trial started onsensor Adafruit,
(DS18S20;New York,New
Adafruit, NY).York,
The assembling
NY) instructions
March
and an1st, air 2016 (day 1),and
temperature and relative
5,000 first and second
humidity sensor (AM2302;and basic programming
Adafruit, New York, areNY).
available
The in Ferrarezi et
instar larvae instructions
assembling was added on andMarch
basic8th, 2016 (day are
programming al. (2015).
8). available The system
in Ferrarezi et al. was monitoring
(2015). The the data every
system was monitoring the data every
The BSF growing unit media was composed of 60% 15 minutes and recording the data automatically using
15 minutes and recording the data automatically
Wi-Fi in a Google
woodchips Sheets spreadsheet
(B&T Shavings, Jasper, AL) (Google,
and 40% Mountain View,
usingCA).
Wi-Fi in a Google Sheets spreadsheet (Google,
peat moss (Lambert Peat Moss Inc., Riviere-Ouelle, Mountain View, CA).
Quebec, Canada), creating a moistened environment
with the addition of 7 L/day of water. RESULTS AND DISCUSSION
RESULTS AND DISCUSSION From day 1, the BSF were fed with vegetable
Feed waste (lettuce and squash leaves) and 2-3 L of water
We provided a diet using only vegetable waste were sprayed twice a week. From day 16, we started
(lettuce, squash) every 5 days in the beginning of the adding a source of protein (1.5 to 2 kg of tilapia) to
trial. After two weeks, we started adding fish to the supplement the diet because the larvae 5 were not eating
diet (tilapia, 1.5 to 2 kg), cafeteria scrap (pasta, rice, the vegetables. Tilapia was added twice a week. From
meat, and salad), and water with the same frequency. day 23, we started adding cafeteria scraps (mix of
However, that approach resulted in the attraction of pasta, rice, meat and salad) with the same frequency.
other flies because the BSF were not eating all the The change in diet resulted in maintenance of larvae
organic material available. On day 16 we started production.
feeding the BSF only with 1.5 to 2 kg of tilapia and The first pre-pupa collection was on day 21. In the
on day 29 we started applying 7 L of water twice per next weeks the BSF pre-pupa number collected were
week (Table 1). During the development of the trial irregular, ranging from 5 to 1,120 (Figure 3). During
we added ground coffee in the media. this period, BSF continued to be fed with the same
amount of fish and cafeteria food.
Procedures The uneaten feed was attractive for other types of
Both sides of the growing unit were checked daily flies, especially maggots (order Diptera, in particular
and the BSF pre-pupae were collected. If another type larvae of Brachyceran flies, such as houseflies,
of fly was found inside the system, it was manually cheese flies, and blowflies). From day 25 to 29, the
removed. The pre-pupae collected were counted and trial recorded a high amount of maggots and larvae.
weighed and returned to a 3.78-L bucket (Sterilite, On day 29, we collected 95 g of maggots. That was
Fitchburg, MA) inside the growing unit to allow a high amount when compared with 160 g of BSF
metamorphosis into the adult phase and generation pre-pupa collected at the same day. The pre-pupa
of more flies. We also added 7 L of water daily to collected presented the highest value reported in
37
Figure 3. Number and weight of pre-pupa collected overtime. The large amount of pre-pupa collected on day 29 was
due to the initial larvae added to the system. The peaks of pre-pupa collection are related to the larvae cycle.
Temperature monitoring
The air temperature inside the growing unit
presented a minimum of 21.6°C and maximum of
32.94°C following the day and night temperature
variation. The same trend can be seen for the compost
temperature with minimum of 28.75°C and maximum
of 31.81°C (Figure 4A). The humidity sensor has
recorded minimum value of 58.8% and maximum
value of 99.% (Figure 4B). Calculated vapor pressure
deficit (VPD) ranged from 0.03 to 2.04 kPa (Figure
Figure 4. The air and compost temperature (A) and rela- 4B).
tive humidity and vapor pressure deficit (VPD) (B) over
time. Practical recommendations
For system assembling, we recommend the use of
plywood sheets. All the materials should be secured
the trial. The maggots have a shorter life cycle than to avoid open spaces, reducing the entrance of other
BSF and colonized all the growing unit. We had to insects or animals inside. It is recommended painting
reduce the food source from cafeteria scraps and the outside of the growing unit using white paint to
vegetables to only fish, and added more media (wood reduce the growing unit temperature. Drill a few small
chips) to the growing unit. The number of maggots holes (2 × 2 mm) on the unit bottom to avoid water
started to decrease, and by the end of the month, the accumulation.
38
Related to the BSF growing unit management, source microcontrollers. HortTechnology 25(1):
feed the BSF at libitum, adding small amounts of 110-118.
feed. If the BSF are eating all the food in less than 1 Indexmundi. 2016. Fishmeal monthly price. May
h provide more in the next meal. Make sure the BSF 31, 2016. Available at Available at: http://www.
growing unit is completely sealed, with no openings indexmundi.com/commodities/?commodity=fish-
to other flies or animals — especially lizards that can meal&months=120.
eat all the BSF larvae. After the pre-pupa reach the Msangi, S., M. Kobayashi, M. Batka, S. Vannuccini,
adult stage, throw away the larva skin from the bucket M. Day, and J.L. Anderson. 2013. Fish to 2030:
inside the growing unit since they can attract ants and Prospects for fisheries and aquaculture.
other insects. Moisten the media in the growing unit Washington DC: World Bank Group. World Bank
regularly (twice a week). Report 83177, vol. 1, 102 p.
Naylor, R.L., R.J. Goldburg, J.H. Primavera, N. Kautsky,
CONCLUSIONS M.C.M. Beveridge, J. Clay, C. Folke, J. Lubchenco,
During 90 days we were able to produce 2,080 H. Mooney, and M. Troell. 2000. Effect of
larvae, generating 318.5 g of BSF from 22.794 kg aquaculture on world fish supplies. Nature 405,
of compost and 4 m3 total available space. This 1017-1024.
amount of larvae produced was not economical. Sheppard, C. 1983. House fly and lesser fly control
Approximately 5 kg/day of larvae is needed to utilizing the black soldier fly in manure
supplement 10% of the feed requirements of the management systems for caged laying hens.
CAS. The production of the larvae is possible but Environmental Entomology 12(5): 1439-1442.
the economy of scale to generate enough larvae to Stamer, A., S. Wessels, R. Neidigk, and G. Hoerstgen-
supplement the tilapia feed needs to be determined. Schwark. 2014. Black Soldier Fly (Hermetia
illucens) larvae-meal as an example for a new
ACKNOWLEDGEMENTS feed ingredients’ class in aquaculture diets.
We thank Amro Mustafa, Donna Gonzales, Luis p.1043-1046. In: Rahmann G. and U. Aksoy (Eds.).
Carino Jr., and Micaiah Forde (Horticulture and Building Organic Bridges. Proceedings of the 4th
Aquaculture program) for their technical assistance. ISOFAR Scientific Conference. Organic World
Funding for this research was provided by USDA- Congress 2014, 13-15 Oct., Istanbul, Turkey.
NIFA-Hatch Funds. St-Hilaire, S., C. Sheppard, J.K. Tomberlin, S. Irving,
L. Newton, M.A. McGuire, E.E. Mosley, R.W.
BIBLIOGRAPHY Hardy, and W. Sealey. 2007. Fly Prepre-pupae
Bondari, K. and D.C. Sheppard. 1981. Soldier fly as a Feedstuff for Rainbow Trout, Oncorhynchus
larvae as feed in commercial fish production. mykiss. Journal of the World Aquaculture Society
Aquaculture 24 (1-2): 103-109. 38(1): 59-67.
Bondari, K. and D. C. Sheppard. 1987. Soldier fly, Yu, G.H., Y.H. Chen, Z.N. Yu, and P. Cheng. 2009.
Hermetia illucens L., larvae as feed for channel Research progress on the larvae and prepare-
catfish, Ictalurus punctatus (Rafinesque), and pupae of black soldier fly Hermetia illucens
blue tilapia, Oreochromis aureus (Steindachner). used as animal feedstuff. Chinese Bulletin of
Aquaculture Research 18(3): 209-220. Entomology 46(1): 41-45.
Diener, S., N.M.S. Solano, F.R. Gutiérrez, C. Zurbrügg,
K. Tockner. 2011. Biological treatment of
municipal organic waste using black soldier fly
larvae. Waste and Biomass Valorization 2(4): 357-
363.
Erickson M.C., M. Islam, C. Sheppard, J. Liao, M.P.
Doyle. 2004. Reduction of Escherichia coli O157:
H7 and Salmonella enterica serovar enteritidis in
chicken manure by larvae of the black soldier fly.
Journal of Food Protection 67(4): 685-690.
Ferrarezi, R.S., S.K. Dove, and M.W. van Iersel. 2015.
An automated system for monitoring soil moisture
and controlling irrigation using low-cost open-
39
FACULTY AND STAFF
Administration UVI Student Employees
Robert W. Godfrey, Director Ezron Brooks, Agronomy
Thomas W. Zimmerman, Assistant Director Serena Joseph, Animal Science
Fiola Alexander, Administrative Specialist I Juan Martinez1, Animal Science
Jacqueline Romer, Administrative Specialist I Amran Nero, Animal Science
Angel Gonzalez, Trades Leader Gilbert Roberts, Animal Science
Jose Herrera, Agriculture Aide – Trades leader Devon Bracey, Biotechnology & Agroforestry
Imani Dailey, Biotechnology & Agroforestry
Faculty Shamali A. Dennery, Biotechnology & Agroforestry
Robert W. Godfrey, Professor - Animal Science Kenya M. Emanuel2, Biotechnology & Agroforestry
Thomas W. Zimmerman, Associate Professor - Samuel Joseph, Biotechnology & Agroforestry
Biotechnology & Agroforestry Tyrone R. Pascal2, Biotechnology & Agroforestry
Rhuanito Ferrarezi, Assistant Professor – Horticulture Seti Balkaran2, Horticulture & Aquaculture
& Aquaculture Jomanni Bernier, Horticulture & Aquaculture
Stuart A. Weiss, Assistant Professor - Agronomy Kalunda Cuffy, Horticulture & Aquaculture
Micaiah Forde, Horticulture & Aquaculture
Professional Staff Jayar Greenidge, Horticulture & Aquaculture
Michael J. Morgan, Research Specialist - Agroforestry Amro Mustafa, Horticulture & Aquaculture
Donald Bailey, Research Specialist - Aquaculture
1Visiting student from Texas A&M University - Kingsville
Kenneth Beamer, Research Analyst - Agronomy
2Graduated with Bachelor’s degree in Spring 2016
Sue A. Lakos, Research Analyst - Animal Science
Henry C. Nelthropp, Research Analyst – Animal
Science Graduate Students
Carlos Mantilla, Research Analyst - Biotechnology Lorenzo Cannella3, Horticulture & Aquaculture
Thomas Geiger, Research Analyst - Horticulture Abdel R. A. Nassef4, Horticulture & Aquaculture
3Graduate Exchange Student - University of Genoa
Field Staff 4Graduate Exchange Student - University of Cairo
William Gonzales, Research Assistant - Animal Science
Donna Gonzales, Research Assistant - Aquaculture Sabbatical Visitor
Henry Harris, Research Assistant - Biotechnology Andrew Riseman, PhD - Associate Professor, UBC
Nelson Benitez, Agriculture Aide - Agronomy Centre for Sustainable Food Systems-UBC Farm,
Ephraim Rodriguez, Agriculture Aide - Agronomy Faculty of Land and Food Systems, The University
Royson Joseph, Agriculture Aide - Animal Science of British Columbia, Vancouver, Canada –
Jose Torres, Agriculture Aide - Animal Science Horticulture & Aquaculture
Ismael Montes, Agriculture Aide - Animal Science
Luis Carino, Jr., Agriculture Aide - Aquaculture
Raheem Smart, Agriculture Aide - Biotechnology
James Gordon, Agriculture Aide - Biotechnology
Naima Jenkins, Agriculture Aide - Horticulture
Victor Almodovar, Agriculture Aide – Horticulture
44
© 2016
Argicultural Experiment Station
Dr. Robert W. Godfrey, Director