Project

INVESTIGATORY PROJECT
WORK
Submitted By
ARCHIT
Class: XII A Science
Under the Guidance of

Mr. Mahendra Kalra
PGT (Chemistry)
Department of Chemistry
Kendriya Vidyalaya Itarana.
Department of
Chemistry Kendriya
Vidyalaya Itarana.
CE R T I F I C A T E
This is to certify that ARCHIT of Class XII A Science has prepared the
investigatory chemistry project entitled “To Study the digestion of
starch by salivary amylase and effect of temperature and pH on it”.
The report is the result of his efforts & endeavors.
The report is found worthy of acceptance as final project report for the
subject Chemistry of Class XII. He has prepared the report under my
guidance.
(Mr. Mahendra kalra)

PGT (Chemistry) Department
of Chemistry Kendriya
Vidyalaya Itarana.
Department of
Chemistry Kendriya
Vidyalaya Itarana
CERTIFICATE
The investigatory project entitled
“To Study the digestion of starch by salivary amylase and effect of
temperature and pH on it"
Submitted by Archit of Class XII A Science for the C.B.S.E Senior

Secondary Examination class XII of Chemistry Practical at Kendriya
Vidyalaya Itarana has been examined.
SIGNATURE OF EXAMINER
DECLARATION
I hereby declare that the project work entitled “To

Study the digestion of starch by salivary amylase
and effect of temperature and pH on it”, submitted
to Department of Chemistry, Kendriya Vidyalaya
Itarana is prepared by me.
ARCHIT
Class XII A Science

A C K N OW L E D G E M E NT
I would like to express a deep sense of thanks & gratitude to my

project guide Mr. Mahendra Kalra Sir for guiding me immensely through
the course of the project. He always evinced keen interest in my work.
His constructive advice & constant motivation have been responsible for
the successful completion of this project.
My sincere thanks go to Shri Manoj Pandey, Our principal sir, for

his co-ordination in extending every possible support for the completion
of this project.
I also thanks to my parents for their motivation & support. I must

thanks to my classmates for their timely help & support for compilation
of this project.
Last but not the least; I would like to thank all those who had
Helped directly or indirectly towards the completion of this project.
ARCHIT
Class: XII A Science
CONTENTS
1. Certificate by Teacher. .
2. Certificate by Examiner.
3. Declaration...........
4. Acknowledgement......
5. Verification...........
6. Objectives of the Project
7. Introduction..........
8. Material Required.....
9. Procedure............
10. Observation..........
11. CONCLUSION........
12. BIBLIOGRAPHY
Objectives of the Project Report
The main objective of this chemistry project report

is “To Study the digestion of starch by salivary
amylase and effect of temperature and pH on
it”and
1) To study digestion of starch by saliva.
2) To study the effect of temperature on the digestion of starch by
saliva.
3) To study the effect of pH on the salivary digestion of starch.
Introduction
Every Health book insists on the chewing of food. The act of

chewing stimulates the excretion of saliva. Saliva mixes up with
the food and helps its digestion. That is, the enzyme ptyalin or
amylase present in human saliva hydrolyzes the big molecules of
food into many molecules. For example starch into mono-
saccnaricies maltose and glucose; proteins into amino acids and
fats into fatty acids and glycerol. Thus saliva not only helps in
digestion of food but convert it into energy generating substances.
Further, enzymes and their activity are very sensitive to
temperature and ph. Even a slight variation in these two factors,
can disturb the action of enzymes. In other words, digestion of
food by salivary amylase is also effected by pH and temperature
and can be verified experimentally. For example, hydrolysis of
starch can be verified by testing it with iodine solution. Starch
forms blue coloured complex with iodine. If no starch is present in
a system it will not give blue colour with iodine.
Material Required
The requirements for experiment of Chemistry

Project Report are as under:
1) Test tubes
2) Test tube stand
3) One dropper
4) Beaker
5) Stop watch
6) Starch and Iodine solution
7) Thermometer
8) Dilute HCL and Dilute NaOH solution.

Chemistry Experiment 1 Procedure
Procedure for Chemistry Experiment 1 is:
1) Collection of Saliva- Rinse mouth throughly with cold

water and ensure that it does not contain any food
particles. Now take about 20ml of lupe warm water in
the mouth and gangle for about three minutes so that
saliva mixes up well with it. Spit this into a beaker.
Filter, if there is any suspended impurity clear filtrate is
saliva solution and contains enzyme ptyalin.
2) Preparation of starch solution - Take about 0.5g of
starch in 100ml beaker and add enough water to make a
paste. Dilute the paste by adding 50ml water and boil for
about 5 minutes.
3) Digestion of starch :-
(a) Take 5ml of the starch solution in a test tube. Add
2ml of saliva solution into it. Mix the solutions well
by shaking the tube carefully and start a step watch.
(b) After one minute take out two drops of the mixture
solution from the test tube with the help of a dropper
and transfer it into another test tube containing about
one 1ml of 1% iodine solution. Note the colour
produced, if any.
(c) Repeat this test after every one minute taking two
drops of the mixture solution and fresh 1% iodine
solution continue until the test shows no blue colour.
Record the time and blue colour intensity.
Observation
Time Passed 1 2 3 4
after Mixing Minute Minute Minute Minute
Colour Deep Blue Light No Blue
Intensity Blue Blue
Absence of blue colour on addition to iodine solution

means absence of starch in the mixture solution. That is
whole of the starch has got digested or hydrolysed.
Chemistry Experiment 2
Procedure: -Effect of temperature on the digestion of starch by

saliva.
1) Take three test tubes and label these 1, 2, and 3.

2) Take 5ml of the starch solution, 2ml of the saliva solution and 5ml
of water in each test tube.
3) Place test tube Number 1 in water at room temperature, test tube
Number 2 in a beaker containing water at 500 C and test tube Number 3
in boiling water.
4) After 5 minutes, observe the colour change on mixing two drops of
the mixture of every tube with one ml of 1% iodine solution. Note the
intensity of blue coloured form.
Conclusion
Starch get hydrolysed by saliva amylase

Chemistry Experiment 3
Procedure: - To study the effect of pH on the salivary digestion

of starch
1) Take three test tubes and label these 1, 2, and 3.

2) Add 5 ml of the starch solution, 2ml of the saliva solution in
each test tube.
3) Now ass 2ml of water in test tube Number 1 2ml of Dilute
HCL in test tube Number 2 and 2ml of Dilute NaOH solution in
test tube Number 3 and shake carefully.
4) Keep the three test tubes in water at room temperature for
about 10 minutes.
5) Add 2 drops of the solution of each test tube with 1% iodine
solution and observe the colour change
Conclusion
Temperature effects the digestion of starch by salivary with

increase in temperature salivary amylase get inactivated and
process of digestion do not take place.
BIBLIOGRAPHY
1 http://www.google.com/
2 http://en.wikipedia.org
3 N.C.E.R.T Class 12 Chemistry
4 Dinesh Chemistry
5 Comprehensive Chemistry

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INVESTIGATORY PROJECT

Under the Guidance of

(Mr. Mahendra kalra)

Submitted by Archit of Class XII A Science for the C.B.S.E Senior

I hereby declare that the project work entitled “To

Class XII A Science

I would like to express a deep sense of thanks & gratitude to my

My sincere thanks go to Shri Manoj Pandey, Our principal sir, for

I also thanks to my parents for their motivation & support. I must

6. Objectives of the Project

The main objective of this chemistry project report

Every Health book insists on the chewing of food. The act of

The requirements for experiment of Chemistry

2) Test tube stand

6) Starch and Iodine solution

8) Dilute HCL and Dilute NaOH solution.

Procedure for Chemistry Experiment 1 is:

1) Collection of Saliva- Rinse mouth throughly with cold

Absence of blue colour on addition to iodine solution

Procedure: -Effect of temperature on the digestion of starch by

1) Take three test tubes and label these 1, 2, and 3.

Starch get hydrolysed by saliva amylase

Procedure: - To study the effect of pH on the salivary digestion

1) Take three test tubes and label these 1, 2, and 3.

Temperature effects the digestion of starch by salivary with

3 N.C.E.R.T Class 12 Chemistry

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