Kingfisher

Thermo Scientific
KingFisher Flex
User Manual
Rev. 1.2
Thermo Scientific
KingFisher Flex
User Manual
Rev. 1.2, Cat. no. N07669

Copyright
Copyright © 2010 Thermo Fisher Scientific Corporation. All rights reserved. First edition published in 2008.
Printed in Finland. Reproduction of the accompanying user documentation in whole or in part is prohibited.
Patents
This product is protected by the following patents:
US 6447729, Method and means for magnetic particle specific binding assay, and US 6448092, Separation
device for microparticles involving a magnetic rod.
KingFisher Flex also has national and international patents pending.
Trademarks
“BindIt” is a trademark of Thermo Fisher Scientific. “KingFisher” and “Microtiter” are registered trademarks
of Thermo Fisher Scientific.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of a continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.
No liability for consequential damages

Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to
use this product.
Warranty statement
Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against defective
parts and materials, including defects caused by poor workmanship, for a period of one year from the date of
delivery. Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty
at no extra charge for materials and labor provided that the products were used and maintained in accordance
with Thermo Fisher Scientific’s instructions. The warranty is invalid if products have been misused or abused.
For the warranty to be effective, the product must have been purchased either directly from Thermo Fisher
Scientific or from an authorized Thermo Fisher Scientific distributor. The guarantee is not transferable to a
third party without prior written approval from Thermo Fisher Scientific. This guarantee is subject to the
following exclusions:
● Any defects caused by normal wear and tear.
● Defects caused by fire, lightning, flood, earthquake, explosion, sabotage, war, riot, or any other occurrence
of the type listed above.
● Refurbished products that are subject to different warranty conditions.
THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES,
INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR A PARTICULAR PURPOSE. The seller is not liable for any loss or damage arising out of or
in connection with the use of the product or other indirect damages. These warranty terms and conditions can
be obtained from your local Thermo Fisher Scientific dealer.
Consumables are not included in the warranty.

About This User Manual
Intended users This user manual is written for the actual end user, for example,
research scientist or laboratory technician, and provides information on
the Thermo Scientific KingFisher Flex, including the installation and
operating instructions.
Read the manual in its entirety before operating the instrument.
How to use this This user manual is designed to give you the information to:
user manual ● Review safety precautions
● Install the KingFisher Flex
● Use the KingFisher Flex in routine jobs – the processing step
● Perform basic cleaning and maintenance procedures
● Troubleshoot the instrument performance
This user manual also describes all the features and specifications of the
KingFisher Flex hardware and onboard software. Refer to Chapter 7:
“Technical Specifications”.
In Chapter 4: “Routine Operation” you find explanations of the
processing principles and procedures and on how to use the KingFisher
Flex internal software.
In Chapter 9: “Troubleshooting Guide” you find explanations of error
and warning messages and a problem-solving guide. The user should be
familiar with the contents of Chapter 5: “Maintenance”. For ordering
information, refer to Chapter 10: “Ordering Information”.
For more For PC software-related issues, refer to the Thermo Scientific BindIt
Software User Manual (Cat. no. N07974).
information
For the latest information on products and services, visit our websites
at:
http://www.thermo.com
http://www.thermo.com/kingfisher
In our efforts to provide useful and appropriate documentation, we

appreciate your comments on this user manual to your local Thermo
Fisher Scientific representative.
Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 3

Safety symbols and markings
Safety symbols These symbols are intended to draw your attention to particularly
important information and alert you to the presence of hazards as
and markings indicated.
Safety symbols and The following symbols and markings appear on the type label and the
markings used on the instrument itself.
KingFisher Flex
Power ON ▲
Power OFF ▲
Warning Hot surface, risk of burns. ▲
Warning Risk of body parts, hair, jewelry or clothing getting caught in

a moving part. ▲
Serial number ▲
Catalog number ▲
Date of manufacture ▲
Consult instructions for use ▲
WEEE symbol This product is required to comply with the European

Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2002/96/EC. ▲
A black label with the following text (Figure 2–4):

WARNING: DISCONNECT SUPPLY BEFORE SERVICING
AVERTISSEMENT: COUPER L'ALIMENTATION AVANT
L'ENTRETIEN ET LE DEPANNAGE
4 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Safety symbols and markings
Warning and other The following symbols and markings appear in this user manual.
markings used in
the documentation
Warning Risk of electric shock. ▲
Warning Biohazard risk. ▲
Warning Risk of injury to the user(s). ▲
Caution Risk of damage to the instrument, other equipment or loss of

performance or function in a specific application. ▲
Note Marks a hint, important information that is useful in the

optimum operation of the system, or an item of interest. ▲

Instrument safety and guidelines for use
Instrument safety ● Always follow basic safety precautions when using the KingFisher
Flex to reduce the risk of injury, biohazardous contamination, fire,
and guidelines or electric shock.
for use ● Read this user manual in its entirety prior to operating the
instrument. Failure to read, understand, and follow the instructions
in the manual may result in damage to the instrument, injury to
laboratory and operating personnel or poor instrument
performance.
● Observe all “Warning”, “Caution”, and “Note” statements as well as
safety symbols and markings on the instrument and in the
documentation.
● The device shall be operated only with software specifically designed
for the device.
● Never open any other covers of the KingFisher Flex than the see-
through lid or the sliding door (Figure 2–3) while the instrument is
plugged into a power source.
● Never force a microplate onto the instrument.
● The KingFisher Flex is intended for laboratory research use only.
Observe proper laboratory safety precautions, such as wearing
protective clothing and following approved laboratory safety
procedures.
● Preventative maintenance instructions should be followed closely to
keep the instrument in the best condition for maximum reliability.
A poorly maintained instrument will not give the best results.
Warning This product contains very strong permanent magnets.

People wearing a pacemaker or metallic prostheses should not use this
product. A pacemaker or prostheses may be affected or damaged if it
comes in close contact with a strong magnetic field. ▲

Contents
Intended users ................................................................................... 3
How to use this user manual ............................................................. 3
For more information ....................................................................... 3
Safety symbols and markings............................................................. 4
Safety symbols and markings used on the KingFisher Flex ............. 4
Warning and other markings used in the documentation ............... 5
Instrument safety and guidelines for use............................................ 6
Chapter 1 Introduction to the KingFisher® Flex........................................................ 13

Intended use.................................................................................... 13
Principle of operation...................................................................... 13
Advantages of using KingFisher Flex ............................................... 14
Chapter 2 Functional Description ............................................................................... 15

Instrument layout............................................................................ 15
Front view.................................................................................... 15
Back / side view............................................................................ 16
KingFisher Flex magnetic particle processor .................................... 16
Principle of magnetic particle processing ......................................... 17
Working with a magnetic rod.......................................................... 18
Collecting magnetic particles........................................................ 18
Releasing magnetic particles ......................................................... 18
Washing magnetic particles .......................................................... 18
Incubation ................................................................................... 18
Changing the volume during the magnetic particle processing ..... 18
Chapter 3 Installation .................................................................................................... 21

Delivery check................................................................................. 21
Unpacking ................................................................................... 21
Checking delivery......................................................................... 22
Requirements .................................................................................. 22
Precautions and limitations ............................................................. 23
Installation setups............................................................................ 23
Releasing the transport locks ........................................................ 23
How to ensure startup.................................................................. 26
How to fit the subassemblies of the instrument into place ............ 27
Heating block ........................................................................... 27
Shield plate ............................................................................... 29
Interchangeable KingFisher Flex heads...................................... 31
See-through lid.......................................................................... 34
Operational check ........................................................................... 34
How to pack the instrument for transportation ............................... 35
How to refit the transport lock of the heating block ..................... 35

Contents
How to refit the transport lock of the tip comb holder................. 36

Do’s and Don’ts of the KingFisher Flex .......................................... 39
Chapter 4 Routine Operation ........................................................................................39

Do ............................................................................................... 39
Don’t ........................................................................................... 39
Switching on ................................................................................... 40
Control panel.................................................................................. 40
Keyboard...................................................................................... 40
Keys .......................................................................................... 40
Display......................................................................................... 41
Navigating ................................................................................... 41
Using KingFisher Flex PC software ................................................. 44
Using internal software.................................................................... 44
Factory / User protocols .................................................................. 44
Selecting the protocol................................................................... 44
Setting the default protocol .......................................................... 45
Instrument options.......................................................................... 47
Device information ...................................................................... 47
Computer interface ...................................................................... 48
Language...................................................................................... 48
Buzzer .......................................................................................... 49
Maintenance protocol .................................................................. 50
How to handle tip combs................................................................ 51
How to start.................................................................................... 54
Changing the heating block............................................................. 58
Shutdown ....................................................................................... 60
Emergency situations ...................................................................... 61
Chapter 5 Maintenance .................................................................................................63

Regular and preventive maintenance ............................................... 63
How to clean the turntable........................................................... 63
How to clean the magnetic rods ................................................ 65
How to clean the shield plate .................................................... 66
Disposal of materials ....................................................................... 66
Decontamination procedure............................................................ 66
Packing for service........................................................................... 67
Service contracts.............................................................................. 68
Disposal of the instrument .............................................................. 68
Chapter 6 Technical Specifications ...........................................................................71

General specifications...................................................................... 71
Performance specifications .............................................................. 71
Safety specifications......................................................................... 72
In conformity with the requirements............................................... 72
Chapter 7 Frequently Asked Questions ......................................................................75

Q&As ............................................................................................. 75

Contents
Chapter 8 Troubleshooting Guide ............................................................................... 79

Error messages and warnings ........................................................... 79
Troubleshooting guide .................................................................... 80
Service request protocol................................................................... 81
Certificate of Decontamination....................................................... 82
Chapter 9 Ordering Information................................................................................... 83

KingFisher Flex ............................................................................... 83
List of accessories and consumables ................................................. 83
List of spare parts ............................................................................ 84
Chapter 10 References .................................................................................................... 85

Keywords for web pages .................................................................. 85
Literature ........................................................................................ 86
Appendix A Certificate of Decontamination................................................................. 89
Appendix B Thermo Scientific KingFisher Flex Feedback Form.............................. 91

Figures
Figures
Figure 1–1. KingFisher Flex magnetic particle processor..............................13
Figure 2–2. KingFisher Flex front view without see-through lid ...................15
Figure 2–3. KingFisher Flex front view with see-through lid and plates......15
Figure 2–4. KingFisher Flex back view...........................................................16
Figure 2–5. KingFisher Flex side view ...........................................................16
Figure 2–6. KingFisher Flex magnetic particle processor..............................17
Figure 2–7. Inverse magnetic particle processing.........................................17
Figure 2–8. A concentration step during magnetic particle processing .......19
Figure 3–9. KingFisher Flex transport locks fitted.........................................24
Figure 3–10. Removing the transport lock of the tip comb holder................24
Figure 3–11. Tip comb holder transport lock removed..................................25
Figure 3–12. Removing the transport lock of the heating block (A)..............25
Figure 3–13. Removing the transport lock of the heating block (B)..............26
Figure 3–14. Mains supply cable and serial cable connected ......................27
Figure 3–15. Inserting the heating block .......................................................27
Figure 3–16. Pressing the heating block into place ......................................28
Figure 3–17. Sectional view of the Microtiter deep well 96 plate (a), deep
well 96 plates of other manufacturers (b, c, d) and the
KingFisher Flex 96 deep well heating block ............................29
Figure 3–18. Shield plate prior to fitting .......................................................30
Figure 3–19. Inserting the shield plate..........................................................30
Figure 3–20. Fastening / loosening the shield plate.....................................31
Figure 3–21. Shield plate fitted .....................................................................31
Figure 3–22. KingFisher Flex heads in their storage boxes...........................32
Figure 3–23. Inserting the KingFisher Flex head ...........................................33
Figure 3–24. KingFisher Flex head for KingFisher 24 deep well plate fitted 34
Figure 3–25. See-through lid fitted................................................................34
Figure 3–26. Refitting the transport lock of the heating block (A)................35
Figure 3–27. Refitting the transport lock of the heating block (B)................36
Figure 3–28. Fitting the transport lock of the tip comb holder (A)................37
Figure 3–29. Fitting the transport lock of the tip comb holder (B) ................37
Figure 3–30. Both transport locks refitted.....................................................38
Figure 4–31. Keyboard of the KingFisher Flex ...............................................40
Figure 4–32. Main view on the display of the KingFisher Flex .....................41
Figure 4–33. Tip comb package with two tip combs.....................................51
Figure 4–34. Separating the two tip combs ..................................................52
Figure 4–35. Storing the other tip comb on a KingFisher 96 plate for further
use.............................................................................................52
Figure 4–36. PCR tip comb package ..............................................................53

Figures
Figure 4–37. 24 deep well tip comb package ............................................... 53

Figure 4–38. Combining the tip comb and KingFisher plate (A) ................... 54
Figure 4–39. Combining the tip comb and KingFisher plate (B).................... 55
Figure 4–40. Loading the tip-plate ................................................................ 55
Figure 4–41. Loading the plates .................................................................... 56
Figure 4–42. Plates loaded ............................................................................ 56
Figure 4–43. Tip comb in the tip comb holder............................................... 57
Figure 4–44. Shield plate in function ............................................................ 57
Figure 4–45. KingFisher Flex plates .............................................................. 58
Figure 4–46. Inserting the heating block....................................................... 59
Figure 4–47. Pressing the heating block into place ...................................... 59
Figure 4–48. Four different heating blocks available ................................... 60
Figure 5–49. Unfastening the two finger screws.......................................... 64
Figure 5–50. Removing the turntable............................................................ 64
Figure 5–51. Black spill shield exposed for cleaning.................................... 65

Tables
Tables
Table 3–1. Plates vs. heating adapter(s)........................................................28
Table 3–2. Magnetic head vs. tip comb.........................................................32
Table 4–3. Icons in the main view .................................................................43
Table 4–4. Processing volumes vs. plate types and magnetic heads...........58
Table 4–5. Heating blocks vs. plate types .....................................................60
Table 6–6. General specifications .................................................................71
Table 6–7. Performance specifications..........................................................71
Table 8–8. Error messages reported..............................................................79
Table 8–9. Actions taken against error messages and warnings.................80
Table 9–10. Codes for products .....................................................................83
Table 9–11. Codes for accessories and consumables...................................83
Table 9–12. Codes for spare parts.................................................................84

Chapter 1
Introduction to the KingFisher®
Flex
Intended use The KingFisher Flex magnetic particle processor (Figure 1–1) is
intended for professional research use by trained personnel. The
instrument is intended for automated transfer and processing of
magnetic particles in a microplate scale. Use for self-testing is excluded.
It is recommended that Good Laboratory Practices (GLP) are followed
to guarantee reliable analyses.
Refer to Chapter 6: “Technical Specifications”.
Principle of The KingFisher Flex magnetic particle processor (Figure 1–1) is

designed for automated transfer and processing of magnetic particles in
operation microplate format.
The patented technology of the KingFisher Flex system is based on the
use of magnetic rods covered with a disposable, specially designed tip
comb and plates. The instrument functions without any dispensing or
aspiration parts or devices.
Samples and reagents, including magnetic particles, are dispensed into
the plates according to the corresponding instructions. The protocol
that is selected by the user via the keyboard and display have already
been preloaded into the onboard software. BindIt Software can be used
to create and run protocols.
Figure 1–1. KingFisher Flex magnetic particle processor

Introduction to the KingFisher® Flex
Advantages of using KingFisher Flex
Advantages of The KingFisher Flex magnetic particle processor has several operational
advantages:
using KingFisher
● Enables automation of complicated manual steps
Flex
● Enables simultaneous processing and purification
● Binding and elution are enhanced due to the heating option
● Enables concentration of the sample during processing
● Facilitates a good collection of bead-bound sample due to the
efficiency of the magnet
● Enables a quicker reaction and a more efficient wash due to the
technology of moving magnetic particles instead of liquids
● No carryover
● No cross contamination
● Facilitates the whole processing with the aid of an internal
program/BindIt Software.

Chapter 2
Functional Description
Instrument This section shows the front, back and side views of the KingFisher Flex
instrument.
layout
Front view The front views of the KingFisher Flex instrument are shown in
Figure 2–2 and Figure 2–3.
Interchangeable KingFisher Flex head
Heating block Shield plate Plate station (1 – 8)

Display
Turntable
Keypad
Loading station
Figure 2–2. KingFisher Flex front view without see-through lid
See-through lid
Sliding door (open)
Figure 2–3. KingFisher Flex front view with see-through lid and plates

KingFisher Flex magnetic particle processor
Back / side view The back view of the KingFisher Flex instrument is shown in
Figure 2–4 and the side view in Figure 2–5.
Mains power
Cooling-air inlet Type label USB port Warning marking supply connector Cooling-air outlet
Serial port RS-232C ON/OFF switch
Figure 2–4. KingFisher Flex back view
Figure 2–5. KingFisher Flex side view
KingFisher Flex The KingFisher Flex (Figure 2–6) has room for eight plates. The tip
combs are compatible with the plates. During the individual steps, the
magnetic plates are kept stationary, and the only moving assembly is the
particle processing head with tip comb and magnetic rods. The head consists of
two vertically moving platforms. One is needed for the magnetic rods
processor (24 or 96 pieces) and the other one for the plastic tip comb.
Up to eight plates can be simultaneously on the turntable. However,
during one sample processing, the protocol enables the use of more
than eight plates in total. One tip comb contains 24 or 96 tips used for
processing 24 or 96 samples at a time.
Before starting the magnetic particle processing via the keypad and
display, the samples and reagents are dispensed into the plates and the
tip comb is placed onto a KingFisher plate, from which it is
automatically loaded. The plates are placed onto the turntable into the
corresponding plate stations according to the protocol instructions.

Principle of magnetic particle processing
During the operation, the sliding door can be closed or open

(Figure 2–3) or the whole see-through lid can be absent. The closed
door protects the processing against environmental contamination.
The operating principle employed is MPP (inverse magnetic particle
processing) technology (Figure 2–7). Rather than moving the liquids,
the magnetic particles are moved from plate to plate containing specific
reagents, in contrast to the external magnet method. Magnetic particles
are transferred with the aid of magnetic rods covered with a disposable,
specially designed plastic tip comb.
Figure 2–6. KingFisher Flex magnetic particle processor
Principle of magnetic
particle processing
Figure 2–7. Inverse magnetic particle processing

Working with a magnetic rod
Working with a Working with magnetic particles can be divided into five separate
processes:
magnetic rod
● collecting magnetic particles
● releasing magnetic particles
● washing magnetic particles
● incubation
● concentration
Collecting magnetic During the collection of the magnetic particles, the magnetic rod is
particles fully inside the tip. The magnetic rods together with the tip comb move
slowly up and down in the plate and the magnetic particles are collected
onto the edge of the tips. The magnetic rods together with the tip
comb, having collected the magnetic particles, can be lifted out of the
plate and transferred into the next plate.
Releasing magnetic After collection of the magnetic particles, the magnetic rods together
particles with the tip comb are lifted from the plate, the magnetic rods are lifted
off and the tip comb is lowered into the next plate containing a reagent.
Magnetic particles are released by moving the tip comb up and down
several times at considerably high speed until all the particles have been
mixed with the substance in the next reaction.
Washing magnetic Washing the magnetic particles is a frequent and an important

particles processing phase. Washing is a combination of the release and
collection processes in a plate filled with washing solution.
To maximize washing efficiency, the magnetic rods together with the
tip comb are designed to have minimized liquid-carrying properties.
Incubation To keep the magnetic particle suspension evenly mixed in long-running

reactions, the tip comb can be moved up and down in the solution.
Changing the volume The volume of the first plate can be larger than the volume of the next
during the magnetic plate, and this is used for concentration purposes (see Figure 2–8
below).
particle processing

Figure 2–8. A concentration step during magnetic particle processing


Chapter 3
Installation
Delivery check This section covers the relevant procedures to be carried out on receipt
of the instrument.
Unpacking Move the packed instrument to its site of operation. To prevent

condensation, the instrument should be left in its protective, antistatic
plastic wrapping until the ambient temperature has been reached.
Unpack the KingFisher Flex instrument and accessories carefully with
the arrows on the transport package pointing upwards. Remove the
instrument from the package and place it on a level surface. Refer to the
enclosed unpacking instructions.
The following notes and instructions are sent with the instrument and
are immediately available when you open the package:
● Unpacking instructions
● KingFisher Flex Feedback Form
● Warranty Certificate card
● Packing instructions/Packing list
● Thermo Scientific KingFisher Flex User Manual and Quick Reference
Guide
● Transportation discrepancy report
● BindIt Software package
Caution Do not touch or loosen any screws or parts other than those
specifically designated in the instructions. Doing so might cause
misalignment and will void the instrument warranty. ▲
Warning The KingFisher Flex weighs approximately 28 kg [62 lbs.]

without the transport package and should be lifted with care. It is
recommended that two persons lift the instrument together, taking the
proper precautions to avoid injury. ▲
To lift the instrument, put your fingers under the bottom on either
sides and lift it with your back straight.

Installation
Requirements
Retain the original packaging and packing material for future

transportation. The packaging is designed to assure safe transport and
minimize transit damage. Use of alternative packaging materials may
invalidate the warranty. Also retain all instrument-related
documentation provided by the manufacturer for future use.
Checking delivery Check the enclosed packing list against order. In case of any deviations,
contact your local Thermo Fisher Scientific representative or Thermo
Fisher Scientific Oy.
Visually inspect the transport package, the instrument and the
accessories for any possible transport damage.
If the carton has been damaged in transit, it is particularly important
that you retain it for inspection by the carrier in case there has also been
damage to the instrument.
Neither the manufacturer nor its agents can be held responsible for any
damage incurred in transit, but the manufacturer will make every effort
to help obtain restitution from the carrier. Upon receipt of the carrier's
inspection report, arrangements will be made for repair or replacement.
If any parts are damaged, contact your local Thermo Fisher Scientific
representative or Thermo Fisher Scientific Oy.
Requirements When you set up your KingFisher Flex, avoid sites of operation with
excess dust, vibrations, strong magnetic fields, direct sunlight or UV
light, draft, excessive moisture or large temperature fluctuations.
● Make sure the working area is flat, dry, clean and vibration-proof
and leave additional room for accessories, cables, and reagent
bottles.
● Make sure the ambient air is clean and free of corrosive vapors,
smoke and dust.
● Make sure the ambient temperature range is between +5°C (41°F)
and +40°C (104°F).
● Make sure relative humidity is between 10% and 80% (non-
condensing).
Leave sufficient space (at least 10 cm) on both sides and at the back of
the unit to allow adequate air circulation.
The KingFisher Flex does not produce operating noise at a level that
would be harmful. No sound level measurements are required after
installation.
Caution Do not operate the instrument in an environment where

potentially damaging liquids or gases are present. ▲

Installation
Precautions and limitations
Place the instrument on a normal laboratory bench. The net weight of

the entire equipment is approx. 28 kg [62 lbs.].
The instrument operates at voltages of 100 – 240 Vac and the
frequency range of 50/60 Hz.
Precautions and ● Always ensure that the local supply voltage in the laboratory
conforms to that specified on the type label on the back of the
limitations instrument (Figure 2–4).
● Do not smoke, eat or drink while using the KingFisher Flex.
● Wash your hands thoroughly after handling test fluids.
● Observe normal laboratory procedures for handling potentially
dangerous samples.
● Wear proper protection clothing, such as disposable gloves and
laboratory coats, according to good laboratory practice.
● Ensure that the working area is well ventilated.
● Never spill fluids in or on the equipment.
Caution The KingFisher Flex should not be kept in close proximity to

magnetic tapes, computer discs or other magnetic storage systems, such
as credit cards, as these can be damaged by the strong magnetic field of
the KingFisher Flex heads.
Do not hold the KingFisher Flex heads close to a PC display, since this
may cause damage to the display.
Do not use metal tools when handling KingFisher Flex heads. ▲

Installation This section describes the installation setups that you must carry out
before operating or relocating the instrument.
setups
Releasing the The instrument comes with two transport locks (Figure 3–9): the tip
transport locks comb holder transport lock and the heating block transport lock.
Remove both the transport locks.
Make sure both the transport locks are released before you put the
instrument into operation.

Installation
Installation setups
To remove both the transport locks, follow these steps:
Tip comb holder

transport lock
Heating block
transport lock
Figure 3–9. KingFisher Flex transport locks fitted

1. To remove the transport lock of the tip comb holder, take firmly
hold of the lock and press tightly on both sides of the lock
according to Figure 3–10 so that the lock ends are released. Keep
hold of the tip comb holder and lock simultaneously and start
gradually lifting the holder.
Figure 3–10. Removing the transport lock of the tip comb holder
2. Lift the tip comb holder higher and continue pressing the transport
lock of the tip comb holder. Remove the transport lock from its

Installation
Installation setups
location by ejecting it from the two eccentric pins (Figure 3–11).

When relocating the instrument, refer to “How to refit the
transport lock of the tip comb holder” on page 36.
Two eccentric pins
Figure 3–11. Tip comb holder transport lock removed
3. The transport lock of the heating block is removed according to

Figure 3–12. Unscrew the transport lock screw counterclockwise
(Figure 3–12). Lift the screw with the label attached (Figure 3–13).
Figure 3–12. Removing the transport lock of the heating block (A)

Installation
Installation setups
4. Remove the transport lock screw from the label (Figure 3–13).
Figure 3–13. Removing the transport lock of the heating block (B)
5. Then fasten the screw clockwise to its storage site on the heating
block base. The two screws on the heating block base guide the
heating block into the correct position.
Note Keep the transport locks (Figure 3–11 and Figure 3–13) for
future transportation of the instrument. ▲
Both the transport locks have been successfully removed. When

relocating the instrument, refer to “How to refit the transport lock
of the heating block” on page 35 and “How to refit the transport
lock of the tip comb holder” on page 36.
How to ensure 1. Connect the mains supply cable to the mains power connector
startup (Figure 3–14) at the bottom left of the back/side panel. If you need
to use any other type of mains supply cable than supplied, use only
cables certified by the local authorities. Before you plug in the
power cable, ensure that the voltage on the type label at the bottom
left of the back/side panel (Figure 2–4) corresponds to the local
voltage.

Installation
Installation setups
2. Connect the instrument to a correctly installed line power outlet

that has a protective conductor that is grounded.
Warning Ensure that the mains switch (Figure 2–4) on the bottom left
of the back/side panel is in the OFF position. Never operate your
instrument from a power outlet that has no ground connection. ▲
Caution Always connect or disconnect the serial cable when the power
is off. ▲
Figure 3–14. Mains supply cable and serial cable connected
How to fit the subassemblies This section describes the installation setups of the heating block, shield
of the instrument into place plate, interchangeable KingFisher Flex heads and the see-through lid.
Heating block Fastening/changing the heating block is controlled by the software. To

fit the heating block, choose the Change_heatblock protocol under the
Maintenance menu by using the up and down cursor keys. Then press
START. The heating block will thus rise. Insert the heating block by first
setting it and then pressing it hard into place (Figure 3–15 and
Figure 3–16). The heating block will snap at both ends if you have
done it correctly.
Figure 3–15. Inserting the heating block

Installation
Installation setups
Figure 3–16. Pressing the heating block into place

You can add a heating step of ambient temperature +5°C to +115°C to
a protocol. The heating block is preheatable but no cooling can be
carried out. All KingFisher Flex plates can be heated.
There are four different kinds of interchangeable heating blocks
available, for KingFisher 24 deep well plates, Microtiter deep well 96
plates, KingFisher 96 plates, and PCR plates (Table 3–1).
Table 3–1. Plates vs. heating adapter(s)
Plate Description Heating adapter(s) Description
KingFisher 24 deep well plate Heating block for KingFisher 24 deep
(200 µl – 5 mL*) well plate
Microtiter deep well 96 plate Heating block for Microtiter 96 deep

(50 – 1000 µl*) well plate
KingFisher 96 plate (20 – 200 µl*) Heating block for KingFisher 96 plate
PCR plate (20 – 100 µl*), skirted Heating block for PCR plate
(for example, AB gene
SuperPlate # AB-2800)
* = recommended filling volume

Installation
Installation setups
Warning The heating block surface can be hot, whereby there can be
risk of burns. ▲
Caution The heating block is specifically designed for the plates listed
below to ensure even heating during the sample process (Figure 3–17).
Using other plates than those recommended may damage the
instrument and diminish the application performance. ▲
A B C D
Figure 3–17. Sectional view of the Microtiter deep well 96 plate (a), deep
well 96 plates of other manufacturers (b, c, d) and the KingFisher Flex 96
deep well heating block
You can only use the following 24 and 96-well plates for heating:
● KingFisher 24 deep well plate (200 µl – 5 mL*)
● Microtiter deep well 96 plate (50 – 1000 µl*)
● KingFisher 96 plate (20 – 200 µl*)
● PCR plate (20 – 100 µl*), skirted
Shield plate To install the shield plate, follow these steps:
1. Ensure that the power is switched OFF.
2. The shield plate has to be installed prior to use of the instrument.

Use a hexagonal screwdriver (Allen key) to fasten the two screws
(Figure 3–18).

Installation
Installation setups
Figure 3–18. Shield plate prior to fitting
3. First insert the shield plate into its slot (Figure 3–19).
Figure 3–19. Inserting the shield plate
4. When fastening the two plate retaining screws (Figure 3–20), be

careful not to drop them inside the instrument. Figure 3–21 shows
the shield plate installed.

Installation
Installation setups
Figure 3–20. Fastening / loosening the shield plate
Figure 3–21. Shield plate fitted
Interchangeable There are four kinds of interchangeable KingFisher Flex heads available,
KingFisher Flex heads for KingFisher 24 deep well plates, Microtiter deep well 96 plates,
KingFisher 96 plates, and PCR plates. The KingFisher Flex heads all
have corresponding disposable plastic tip combs (Table 3–2). Note that
KingFisher Flex heads do not fit into the KingFisher 96 and vice versa.
Caution Do not place the KingFisher Flex heads on top of the

instrument or any metal surfaces.
Keep the KingFisher Flex heads always in their respective plastic boxes
when not in use.
It is very important to keep the KingFisher Flex heads away from each
other and other magnets at all times. Clashing of the magnets together
may cause serious damage to the magnets. ▲

Installation
Installation setups
Table 3–2. Magnetic head vs. tip comb

Magnetic head Description Tip comb Description
KingFisher Flex head for KingFisher 24 Tip comb for KingFisher 24 deep
deep well plate well plate
KingFisher Flex head for Microtiter Tip comb for Microtiter deep
deep well 96 plate well 96 plate
KingFisher Flex head for KingFisher 96 Tip comb for KingFisher 96 plate
plate
KingFisher Flex head for PCR plate Tip comb for PCR plate
When not in use, keep the KingFisher Flex heads always in their
respective plastic storage boxes (Figure 3–22).
Figure 3–22. KingFisher Flex heads in their storage boxes

Do not use metal tools when handling KingFisher Flex heads. ▲

Installation
Installation setups

To insert KingFisher Flex heads, choose the Change_magnet protocol

under the Maintenance menu by using the up and down cursor keys.
Then press START. Insert the KingFisher Flex head so that the three
pins (shown with black arrows) slot into place (Figure 3–23 and
Figure 3–24). When you remove the KingFisher Flex head, be careful
not to damage the magnet rods against the tip comb holder frame.
Figure 3–23. Inserting the KingFisher Flex head

Installation
Operational check
Figure 3–24. KingFisher Flex head for KingFisher 24 deep well plate fitted
See-through lid During operation, the sliding door can be open or closed or the whole
see-through lid can be absent (Figure 3–25). The closed door protects
the processing against environmental contamination.
See-through lid
Sliding door (open)
Figure 3–25. See-through lid fitted
Operational First switch the instrument ON. The instrument performs initialization
tests and adjustments.
check
The display quickly shows the internal software version. This happens
when the initialization tests and adjustments have been completed.

Installation
How to pack the instrument for transportation
It is recommended that you carry out a check run using a demo

protocol to verify proper instrument operation. Run the check protocol
(Check_96dw_tip, Check_KF96_tip, Check_pcr_tip, or
Check_24dw_tip) under the Maintenance menu according to the
KingFisher Flex head and plastics you are using. If the check is all right,
proceed with your own runs.
How to pack the This section describes how to refit both the transport locks, the
transport lock of the heating block and that of the tip comb holder.
instrument for
transportation
How to refit the To refit the transport lock of the heating block, follow these steps:
transport lock of the
heating block 1. Remove the heating block in the following way:
• First choose the Change_heatblock protocol.
• Then press START.
• Remove the heating block by pulling it out.
• Press the START key when the task has been completed.
2. Switch off the power.
3. Take the transport lock screw from its storage site.
4. Insert the transport lock screw into the label and refit the transport
lock of the heating block into its place (Figure 3–26).
Figure 3–26. Refitting the transport lock of the heating block (A)

Installation
5. The transport lock of the heating block is refitted according to

Figure 3–27. Screw the transport lock screw clockwise
(Figure 3–27). Figure 3–30 shows both the transport locks refitted.
Figure 3–27. Refitting the transport lock of the heating block (B)
How to refit the To refit the transport lock of the tip comb holder, follow these steps:
transport lock of the
tip comb holder 1. The transport lock of the tip comb holder is refitted according to
Figure 3–28 through Figure 3–29. Press the transport lock from
both sides and insert the two eccentric pins back into place into
their respective holes (Figure 3–28).
Caution Ensure that the power is switched off. ▲

Installation
Figure 3–28. Fitting the transport lock of the tip comb holder (A)
2. Press the tip comb holder transport lock ends together with both
hands and insert all the way down to lock position (Figure 3–29).
Note that the turntable can move slightly although the transport
lock is fitted.
Figure 3–29. Fitting the transport lock of the tip comb holder (B)

Installation
Figure 3–30. Both transport locks refitted

Figure 3–30 shows both the transport lock of the heating block and the
tip comb holder refitted.

Chapter 4
Routine Operation
Do’s and Don’ts of the This section on Do’s and Don’ts summarizes all the relevant procedures
on what to do and what not to do.
KingFisher Flex
Do ● In case of any emergencies occurring during operation, switch off
and unplug the instrument immediately. Carry out corrective
measures. If the corrective measures taken do not help, contact
authorized technical service.
● Carry out the operational check prior to normal use.
● Only when the instrument is switched off, can you push the heads
or turn the turntable manually.
● When placing a microplate onto the tray, always make sure that the
correct plate type has been selected in BindIt Software (Plate Editor
> Plate Configuration…) before you do anything else.
● Always use correct volumes on the plates because the movements of
the tip comb are conducted according to the volumes.
Don’t ● Use for self-testing is excluded.

● Do not touch or loosen any screws or parts other than those
specifically designated in the instructions. Doing so might cause
misalignment and will invalidate the instrument warranty.
● Never open any other cover of the KingFisher Flex than the see-
through lid or the sliding door (Figure 2–3) while the instrument is
plugged into a power source.
● Do not disconnect the RS-232C or USB cable before the PC and
the instrument are switched off.
● Do not use the instrument if it appears that it does not function
properly.
● Do not under any circumstances use formaldehyde.
● Do not spill any alkalines onto any instrument surfaces to avoid
damage of the instrument. If needed, use suitable protection
covering.

Routine Operation
Switching on
Switching on Before you switch on the KingFisher Flex, ensure that the voltage on
the type label at the bottom left of the back/side panel (Figure 2–4)
corresponds to the local voltage.
Warning Never operate your instrument from a power outlet that has
no ground connection. ▲
Control panel This section describes the KingFisher Flex control panel and internal
software.
Keyboard The keyboard is shown in Figure 4–31.
Arrow keys and OK button
START, PAUSE and

STOP function buttons
TURNTABLE ROTATING
CLOCKWISE and
COUNTERCLOCKWISE
buttons
Figure 4–31. Keyboard of the KingFisher Flex

Keys The relevant keys and control buttons are described in detail below.
The arrow keys are used to select the next protocol and to navigate in
the display.
To accept the selection.
To initiate the run.

To confirm a performed step in the protocol, for example, plate
loading or removal.
To pause/terminate the processing step. In short:

STOP (paused)/ START (the instrument continues after a Pause step)
STOP (paused)/ STOP (the processing is terminated).

Routine Operation
Control panel
To pause the run. It will pause at the end of the ongoing processing
step.
To rotate the turntable clockwise (TURNTABLE ROTATING CLOCKWISE

button).
To rotate the turntable counterclockwise (TURNTABLE ROTATING

COUNTERCLOCKWISE button).
Display The liquid crystal display is a 240 x 360 pixel display.

The main view in the display is shown in Figure 4–32.
Menu
Main view row

Icon
Info text bar
Figure 4–32. Main view on the display of the KingFisher Flex
There are three menus in the KingFisher Flex user interface: Factory
protocols, User protocols and Maintenance. In routine use you
mainly navigate in the Factory/User protocols menu. In advanced
level options there is one extra menu, Maintenance. You can navigate
between these three menus using the Left and Right arrow keys.
The main view row is either colored (active) or uncolored/different

colored (inactive).
All the descriptive icons used in the main view are shown in Table 4–3
below.
The info text bar shows explanatory information on how to proceed and
which keys to use.
Navigating This section visualizes navigation in the KingFisher Flex user interface.
The main view changes according to the selections you make either
with the Up or Down arrow keys or the OK button. The available
buttons and their function are shown on the info text bar.

Routine Operation
Control panel
Active item
The color of the items, for example, the icon and main view row, in the
main view changes when they are selected (active/inactive).
To move from one menu to another, make sure you are in the main
view of one of the menus and use the Left and Right arrow keys.
The main views of each menu tab are shown below.
Factory protocols User protocols Maintenance

Routine Operation
Control panel
Table 4–3. Icons in the main view

Menu Icon Function
“Setting the default protocol” on page 45
/
Last used Default
“Selecting the protocol” on page 44
DNA/RNA
Cell
Protein
Other
“Setting the default protocol” on page 45

/
Last used Default
“Selecting the protocol” on page 44
DNA/RNA
Cell
Protein
Other
“Device information” on page 47
“Computer interface” on page 48
“Language” on page 48
“Buzzer” on page 49
“Maintenance protocol” on page 50

Routine Operation
Using KingFisher The operation of the KingFisher Flex magnetic particle processor can
also be controlled by an external computer and run on BindIt Software.
Flex PC software In addition to the KingFisher Flex internal software features, you can
also download protocols to the instrument or back up protocols from
one instrument and transfer them to another.
For more information, refer to the BindIt Software User Manual
(Cat. no. N07974).
Using internal This section describes procedures related to the KingFisher Flex internal
software.
software
Factory / User This section describes the factory/user set protocols. They are found in
the Factory protocols/User protocols menu. Factory protocols are
protocols protocols which are preloaded into the instrument internal memory in
the factory, for example, demo protocols. User protocols are protocols
which are made and transferred using BindIt Software.
Selecting the protocol Go to the Factory protocols/User protocols menu. Select, for
example, the DNA/RNA row.
Press OK.

Routine Operation
Factory / User protocols
Use the Up and Down arrow keys to select the protocol.
Press START to run the protocol.
Press OK to see the protocol details.
Setting the default Go to the Factory protocols/User protocols menu. You can set your
protocol default protocol. If you do not, the last used protocol will be shown.
To set the default protocol, go to the protocol list Last used/Default
protocol row.
/
Last used
Default
Press OK to start setting your default protocol.

Routine Operation
Factory / User protocols
Press OK to select your default protocol.
Use the Up and Down arrow keys to select the protocol.
Press OK to set the default protocol.
Press STOP to go back.

Routine Operation
Instrument options
Instrument This section describes the instrument parameters. All these parameters
are set in the Maintenance menu. The values shown in the
options Maintenance menu remain in the instrument memory and are
instrument specific, not protocol specific.
Device information The device information shows the name of the instrument, the internal
software version and the serial number of the instrument unit.
Go to the Maintenance menu.
Press OK.

Routine Operation
Instrument options
Press OK to exit.
Computer interface There are two types of computer interfaces available: RS-232 and USB.
The instrument will automatically connect to the interface that is first
used after the power is switched on.
Go to the Maintenance menu. Select the Computer interface row.
Press OK.
This selection also has to be

done when you use the
instrument through BindIt
Software. Refer to Chapter
2: “Installing the Software”
in the BindIt Software User
Manual (Cat. no. N07974).
Use the Up and Down arrow keys to select the computer interface in
use. Not active will reset the computer interfaces.
Press OK.
Language You can set the language of the internal software.

Go to the Maintenance menu. Select the Language selection row.

Routine Operation
Instrument options
Press OK.
Use the Up and Down arrow keys to select the language in use. The
available languages are English, Deutsch, Français, Español and
Portuguese. The default language is English.
Press OK.
Buzzer You can choose whether or not the instrument produces a sound for
different functions. Note that all functions make a certain sound.
Go to the Maintenance menu. Select the Buzzer on/off row.

Routine Operation
Instrument options
Press OK.
Use the Left and Right arrow keys to select whether the buzzer (audible
sound) is On or Off.
Press OK.
Maintenance protocol You can set which protocol is automatically selected as the maintenance
protocol when the KingFisher Flex is powered on.
Go to the Maintenance menu. Select the Maintenance protocol row.
Press OK. All available maintenance protocols will be displayed.

Routine Operation
How to handle tip combs
Use the Up and Down arrow keys to select the maintenance protocol.
Press START to run the desired protocol.
Press OK to go back.
How to handle To handle 96 deep well tip combs correctly, follow these steps:
tip combs
1. Take a 96 deep well tip comb package containing two tip combs
(Figure 4–33).
Figure 4–33. Tip comb package with two tip combs
2. Unpack the package by separating the two tip combs (Figure 4–34)
and placing one of the tip combs onto the KingFisher 96 plate
(= tip-plate) (Figure 4–38 and Figure 4–39).

Routine Operation
Figure 4–34. Separating the two tip combs
3. Place the other unused tip comb onto another plate for storage to
avoid bending of the tip combs (Figure 4–35) to ensure proper
instrument operation.
Figure 4–35. Storing the other tip comb on a KingFisher 96 plate for further
use
To handle PCR tip combs correctly, follow these steps.
1. Take a PCR tip comb package containing eight tip combs within
each other on a screen plate (Figure 4–36).

Routine Operation
Figure 4–36. PCR tip comb package
2. Unpack the package, take out one PCR tip comb and place it on a
KingFisher plate.
If the tip combs do not load properly, refer to Q16 on page 77.
To handle 24 deep well tip combs correctly, follow these steps:
1. Take a 24 deep well tip comb package where the unattached tip
comb is packed onto a KingFisher 24 deep well plate
(Figure 4–37).
Figure 4–37. 24 deep well tip comb package
2. Unpack the package.
3. Before you take the tip comb into use, press the tip comb evenly
against the enclosed 24 deep well plate with the aid of a magnetic
head or another object.

Routine Operation
How to start
How to start To start the instrument, follow these steps:
1. Select a protocol by using the cursor keys and press START OR use
BindIt Software to run the desired protocol via the PC.
2. Open the sliding door if the see-through lid is in place (Figure 2–3).
3. Load the plates in the order that the protocol requests (Figure 4–40
through Figure 4–42). Place the A1 well of the plate so that it is in
the upper right corner. The first A1 row is consequently always in
the inner circle. Once you have loaded the requested plates into the
plate stations, press START. The tip comb always has to be placed
manually onto a KingFisher plate (Figure 4–38 through
Figure 4–40). The instrument also functions with either one plate
or up to eight plates depending on the amount of steps. Only one
tip comb is placed onto a KingFisher plate (= tip-plate) per run
(Figure 4–39). Confirm the plate loading by pressing START.
Figure 4–38. Combining the tip comb and KingFisher plate (A)

Routine Operation
How to start
Plate station 1
Loading postion,
plate station 4
Figure 4–39. Combining the tip comb and KingFisher plate (B)
The loading position, that is, plate station 4, is labeled. The eight plate
stations and the A1 positions of the eight plate stations are clearly
marked on the turntable. When the instrument is in its basic position,
plate station 1 is under the KingFisher Flex head. After the protocol has
been run, note that the turntable may stop in a different position than
the basic position.
A1 corner
Figure 4–40. Loading the tip-plate

Routine Operation
How to start
Tip-plate
Figure 4–41. Loading the plates
Magnetic rods
Tip comb holder
Tip-plate
Figure 4–42. Plates loaded
4. The tip comb is automatically locked onto the tip comb holder
from the tip-plate (Figure 4–43).

Routine Operation
How to start
Tip comb in the

tip comb holder
Figure 4–43. Tip comb in the tip comb holder
5. When the turntable moves, the shield plate moves over the plate
underneath (Figure 4–44) forming a protective cover.
Shield plate
Figure 4–44. Shield plate in function
6. Close the sliding door. The see-through lid (if in place) protects the
instrument against environmental contamination.

Routine Operation
Changing the heating block
Note The sliding door can be left open or the see-through lid can be
absent if desired. This action does not break the run. ▲
7. After the run, remove the plate(s) according to the protocol request.
Confirm each plate removal by pressing the START key. Note that
the plate containing your samples is removed first.
8. Press the STOP key after completing the run.

For startup, refer to “Switching on” on page 40.
Figure 4–45. KingFisher Flex plates
Caution Do not use other plates than the ones listed in Table 4–4.
Other plates may not be compatible with the KingFisher Flex heating
blocks. They may also cause unexpected problems, such as cross-
contamination due to the divergent well volume and bottom height of
the plate. ▲
The KingFisher Flex is compatible with KingFisher 24 deep well plates,

Microtiter deep well 96 plates, KingFisher 96 plates, and PCR plates
(Figure 4–45 and Table 4–4). Specially designed magnetic rods
(KingFisher Flex heads) and tip combs that protect the magnets during
the process are available for different plate types and applications.
Table 4–4. Processing volumes vs. plate types and magnetic heads
Head / Plate KingFisher 24 Microtiter deep KingFisher 96 PCR plate,
deep well plate well 96 plate plate skirted
KingFisher Flex head for 200 – 5000 µl – – –
KF 24 deep well plate
KingFisher Flex head for – 50 – 1000 µl 50 – 150 µl –
Microtiter deep well 96 plate
KingFisher Flex head for KF 96 plate – – 20 – 200 µl –
KingFisher Flex head for PCR plate – – 20 – 200 µl 20 – 100 µl
Changing the Changing the heating block is controlled by the software. To change
the heating block, choose the Change_heatblock protocol by using the
heating block up and down cursor keys. Then press START. The protocol requests you
to remove the magnets and in the end to insert them. The heating block
will thus rise. Remove the heating block and then replace it

Routine Operation
Changing the heating block
(Figure 4–46 and Figure 4–47). Insert the heating block by first setting
it and then pressing it hard into place. The heating block will snap at
both ends if you have done it correctly. Press the START key when the
task has been completed.
Figure 4–46. Inserting the heating block
Figure 4–47. Pressing the heating block into place

You can add a heating step of ambient temperature +5°C to +96°C to a
protocol. The heating block is preheatable but no cooling can be carried
out. All KingFisher Flex plates can be heated.

Routine Operation
Shutdown
There are four different kinds of interchangeable heating blocks

available, for KingFisher 24 deep well plates, Microtiter deep well 96
plates, KingFisher 96 plates, and PCR plates (Figure 4–48 and
Table 4–5).
Figure 4–48. Four different heating blocks available
Table 4–5. Heating blocks vs. plate types

Heating block / Plate KingFisher 24 Microtiter deep KingFisher 96 PCR plate,
deep well plate well 96 plate plate skirted
Heating block for KingFisher 24 deep x – – –
well plate
Heating block for Microtiter deep well – x – –
96 plate
Heating block for KingFisher 96 plate – x x –
Heating block for PCR plate – – – x
Warning The heating block surface can be hot, whereby there can be
risk of burns. ▲
Caution The heating block is specifically designed for the plates listed
below to ensure even heating during the sample process (
Figure 3–17). Using other plates than those recommended may damage
the instrument and diminish the application performance. ▲
Shutdown To shut down the KingFisher Flex, follow the steps below:
1. Switch the KingFisher Flex off by pressing the power switch

(Figure 2–4) at the bottom left of the back/side panel of the
instrument into the OFF position.
Warning Discard the plastic tip comb onto the plate. Dispose of all tip
combs as biohazardous waste.
Remove any plates still in the instrument. Dispose of all microplates as
biohazardous waste. ▲

Routine Operation
Emergency situations
2. Wipe the turntable surface and the adjacent instrument surface with
a soft cloth or tissue paper moistened with distilled water, a mild
detergent (SDS, sodium dodecyl sulfate) or soap solution.
3. If you have spilt infectious agents on the turntable, disinfect with

70% ethanol or another disinfectant (see “Decontamination
procedure” on page 66).
Emergency In case there is any abnormal situation during operation, such as fluids
spilling inside the instrument, follow the steps below:
situations
1. Switch OFF the instrument (Figure 2–4).
2. Unplug the instrument immediately from the power supply

(Figure 2–4).
3. Carry out appropriate corrective measures. However, do not

disassemble the instrument.
4. If the corrective measures taken do not help, contact authorized

technical service or your local Thermo Fisher Scientific
representative.

Routine Operation
Emergency situations

Chapter 5
Maintenance
Regular and For reliable daily operation, keep the instrument free of dust and liquid
spills.
preventive
Abrasive cleaning agents are not recommended, because they are likely
maintenance to damage the paint finish.
It is recommended that you clean the case of the instrument
periodically to maintain its good appearance. A soft cloth dampened in
a warm, mild detergent solution will be sufficient.
Clean the outside of the instrument and the turntable with clean low-
pressure compressed air or a cloth dampened with water or a mild
detergent when necessary.
Although the KingFisher Flex is constructed from high-quality
materials, you must immediately wipe away spilt saline solutions,
solvents, acids or alkaline solutions from outer surfaces to prevent
damage.
Caution Painted surfaces can be cleaned with most laboratory

detergents. Dilute the cleaning agent as recommended by the
manufacturer. Do not expose the surfaces to concentrated acids or
concentrated alcohols for prolonged periods of time as damage may
occur. ▲
Clean the display areas with a mild laboratory detergent. The keypad
has a wipe-clean surface.
Plastic covers and surfaces can be cleaned with a mild laboratory
detergent or alcohol.
Warning If any surfaces are contaminated with biohazardous material,

a mild sterilizing solution should be used. ▲
Caution Do not autoclave any part of this instrument except the shield
plate. ▲
How to clean the Keep the turntable surface clean to avoid dust and dirt entering into the
turntable instrument. Clean the turntable surface at least once a week using a soft

Maintenance
Regular and preventive maintenance
cloth or tissue paper soaked in a mild detergent solution (SDS), soap

solution or alcohol.
The turntable can be detached for cleaning purposes (Figure 5–49
through Figure 5–51).
Unscrew the two finger screws (Figure 5–49). Note that the screws still
remain attached to the turntable when the screws are unfastened.
Alignment stud
Figure 5–49. Unfastening the two finger screws

Lift the turntable off (Figure 5–50).
Figure 5–50. Removing the turntable

Maintenance
Regular and preventive maintenance
If you have spilt infectious agents on the turntable, clean it with a cloth
dampened with water or a mild detergent.
Clean the black spill shield (Figure 5–51) and the turntable using a soft
cloth or tissue paper soaked in a mild detergent solution (SDS), soap
solution or alcohol.
Alignment stud
Figure 5–51. Black spill shield exposed for cleaning

When you replace the turntable, insert the turntable so that you place it
first onto the aligning stud. Then fasten the two finger screws.
You can gently rotate the turntable while the instrument is switched off.
How to clean the If required, wipe the magnetic rods using a soft cloth or tissue paper
magnetic rods soaked in a mild detergent solution (SDS), soap solution or alcohol.

Do not use metal tools when handling KingFisher Flex heads.
Be careful not to break the magnets while cleaning. ▲


Maintenance
Disposal of materials
How to clean the shield If required, wipe the shield plate using a soft cloth or tissue paper
plate soaked in a mild detergent solution (SDS), soap solution or alcohol.
The shield plate is autoclavable (at 1 bar pressure and 121°C for
20 minutes).
Disposal of Follow laboratory and country-specific procedures for the disposal of

biohazardous or radioactive waste. Refer to local regulations for the
materials disposal of infectious material.
Warning The samples can be potentially infectious. Dispose of all used

plates, disposable gloves, syringes, disposable tips, and so on as
biohazardous waste. ▲
Decontamination Decontamination should be performed in accordance with normal

laboratory procedures. Any decontamination instructions provided with
procedure the reagents used should be followed.
A decontamination procedure is only recommendable when infectious
substances have been in direct contact with any part(s) of the
instrument.
If there is a risk of contamination with biohazardous material, the
procedure recommended below or some other corresponding
decontamination procedure must be performed.
It is strongly recommended to perform the complete decontamination
procedure before relocating the instrument from one laboratory to
another or before sending it to service.
Decontamination is not required for the proper functioning of the
instrument.
Example of decontaminants:
● Ethanol 70%
● Virkon solution 1 – 3%
● Glutaraldehyde solution 4%
● Chloramine T
● Microcide SQ 1:64
Warning The decontamination procedure should be performed by

authorized trained personnel wearing disposable gloves, protective
glasses and clothing in a well-ventilated room. ▲
1. Prepare the decontaminant: 200 ml 4% glutaraldehyde solution (or

another agent recommended by your safety officer).

Maintenance
Packing for service
2. Empty the turntable.
3. Switch OFF the power and disconnect the mains supply cable
(Figure 3–14).
4. Disinfect the outside of the instrument using a cloth dampened

with 70% ethanol.
5. Place the instrument in a large plastic bag. Ensure that the see-
through lid has been removed.
6. Place a cloth soaked in the prepared solution into the bag. Ensure
that the cloth does not make contact with the instrument.
7. Close the bag firmly and leave the instrument in the bag for at least
24 hours.
8. Remove the instrument from the bag.
9. Clean the instrument using a mild detergent.
10. Remove any stains using 70% ethanol.
11. After performing this decontamination procedure, enclose a signed

and dated Certificate of Decontamination both inside the transport
package and attached to the outside of the package (see Appendix A:
“Certificate of Decontamination”).
Packing for To pack for service, follow the guidelines presented below.
service
Caution It is important that the instrument is thoroughly
decontaminated before it is removed from the laboratory or any
servicing is performed on it. ▲
When you ship the instrument for service, remember to:

● Inform about the use of hazardous materials.
● Decontaminate the instrument beforehand.
● Install the transport locks.
● Place the KingFisher Flex head into its transportation box.
● Pack the instrument according to the enclosed packing instructions.

Maintenance
Service contracts
● Use the original packaging to ensure that no damage will occur to

the instrument during shipping. Any damage will incur additional
labor charges.
● Enclose a dated and signed Certificate of Decontamination (see
Appendix A: “Certificate of Decontamination”) both inside and
attached to the outside of the package, in which you return your
instrument (or other items).
● Enclose the return goods authorization number (RGA) given by
your Thermo Fisher Scientific representative.
● Indicate the fault after you have been in touch with your local
Thermo Fisher Scientific representative or Thermo Fisher
Scientific’s technical service department.
Refer to “General specifications” on page 71 for details on storage and
transportation temperatures.
Service It is recommended to maintain and service the instrument regularly

every 12 months on a contract basis by the manufacturer's trained
contracts service engineers. This ensures that the product is properly maintained
and gives trouble-free service. Contact the Thermo Fisher Scientific
technical service department for more details.
Disposal of the If the KingFisher Flex has to be disposed of, follow the guidelines
below.
instrument
Warning Decontaminate the instrument before disposal. Refer to
“Decontamination procedure” on page 66 and “Certificate of
Decontamination” on page 82 about decontamination. ▲
Follow laboratory and country-specific procedures for biohazardous or

radioactive waste disposal.
Dispose of the instrument according to the legislation stipulated by the
local authorities concerning take-back of electronic equipment and
waste. The procedures vary by country.
Pollution degree 2 (see “Safety specifications” on page 72)
Method of disposal Electronic waste
Contaminated waste
(Infectious waste)

Maintenance
Disposal of the instrument
WEEE symbol Thermo Fisher Scientific has contracted with one or

more recycling/disposal companies in each EU Member State
(European Country), and this product should be disposed of or recycled
through them. Further information on Thermo Fisher Scientific’s
compliance with these Directives, the recyclers in your country, and
information on Thermo Scientific products which may assist the
detection of substances subject to the RoHS Directive are available at
www.thermo.com/WEEERoHS. ▲
Regarding the original packaging and packing materials, use the

recycling operators known to you.
For more information, contact your local Thermo Fisher Scientific
representative.

Maintenance
Disposal of the instrument

Chapter 6
Technical Specifications
General Thermo Fisher Scientific reserves the right to change any specifications
without prior notice as part of our continuous product development
specifications program. The general specifications are presented in Table 6–6.
Table 6–6. General specifications
General specifications
Overall dimensions
– instrument ca. 680 mm (W) x 600 mm (D) x 380 mm (H)
[26.8” (W) x 23.6” (D) x 15” (H)]
– transport package 800 mm (W) x 1200 mm (D) x 710 mm (H) 31.5” (W) x 47.2” (D) x 28” (H)
Weight
– instrument ca. 28 kg [62 lbs.]
– incl. transport package ca. 44 kg [97 lbs.]
Operating conditions +5°C to +40°C; maximum relative humidity 80% for temperatures up to
(indoor use) 31°C decreasing linearly to 50% relative humidity at 40°C
Indoor use only
Transportation conditions -40°C to +60°C, packed in transport packaging
Storage conditions -25°C to +50°C, packed in transport packaging
Mains power supply 100 – 240 Vac, 50/60 Hz, nominal
Automatic voltage detection
Power consumption 150 VA max.; 55 VA standby
Heat dissipation 341 BTU max.
Computer interface USB or serial RS-232C port. Baud rate 9600.
Character format 1 start bit, 8 data bits, 1 stop bit, and no parity.
Flow control XON/XOFF.
Internal software Space for max. 512 internal protocols
Normal use 10 runs/day, 250 days/year, with 40 min protocols having a medium speed
setting, RT
Performance The performance specifications are presented in Table 6–7.

specifications Table 6–7. Performance specifications
Performance specifications
Processing volume 24: 200 µl – 5 mL
96: 20 – 1000 µl
Continued

Safety specifications
Cont.
Performance specifications
Capacity 96 samples/run
Collection efficiency of > 95%, KingFisher 96 plate, neutral wash buffer containing detergent,
the particles (indoor use) 2.8 µm particles, 3 collections, RT
Magnetic particle size ca. > 1 µm
Magnet rods 24 or 96 in one frame
Interchangeable KingFisher Flex heads
Plate types (disposable) 24 / 96-well plates:
* Recommended filling – KingFisher 24 deep well plate (200 µl – 5 mL*)
volume – Microtiter deep well 96 plate (50 – 1000 µl*)
– KingFisher 96 plate (20 – 200 µl*)
– PCR plate (20 – 100 µl*), skirted
Tip combs (polypropylene 24 / 96 in one frame
– disposable) – for KingFisher 24 deep well plate
– for Microtiter deep well 96 plate
– for KingFisher 96 plate
– for PCR plate
Heating block From +5°C above ambient temperature to +115°C
temperature
Heating block accuracy ± 1°C, up to +80°C, ± 2°C, up to +115°C, instrument in RT
Keyboard / Display START / PAUSE / STOP / OK / TURNTABLE ROTATING CLOCKWISE /
TURNTABLE ROTATING COUNTERCLOCKWISE / four cursor keys / LCD
Safety This section describes the safety specifications for the KingFisher Flex
instrument.
specifications
In conformity KingFisher Flex bears the following markings:
with the Type 711

100 – 240 Vac, 50/60 Hz, 150 VA
requirements
CE mark
CSA
KingFisher Flex conforms to the following requirements:

2006/95/EC (Low Voltage Directive)
2004/108/EC (Electromagnetic Compatibility Directive, EMC)
FCC Part 15, Subpart B/Class B (July 2004)
2002/96/EC (Waste of Electrical and Electronic Equipment)

In conformity with the requirements
Safety performance:
EN 61010-1:2001 (Ed. 2),
including US and CA National differences
EN 61010-2-010:2003 (Ed. 2)
EN 61010-2-101:2002 Particular Requirements for In Vitro Diagnostic (IVD) Medical Equipment
The safety specifications are also met under the following environmental
conditions in addition to or in excess of those stated in the operating conditions:
Altitude Up to 2000 m
Temperature +5°C to +40°C
Humidity Maximum relative humidity 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C
Mains supply ± 10% from nominal
fluctuations
Installation category II according to IEC 60664-1 (see Note 1)
(overvoltage category)
Pollution degree 2 according to IEC 60664-1 (see Note 2)
Note 1) The installation category (overvoltage category) defines the level of

transient overvoltage which the instrument is designed to withstand safely.
It depends on the nature of the electricity supply and its overvoltage
protection means. For example, in CAT II which is the category used for
instruments in installations supplied from a supply comparable to public
mains, such as hospital and research laboratories and most industrial
laboratories, the expected transient overvoltage is 2500 V for a 230 V
supply and 1500 V for a 120 V supply.
2) The pollution degree describes the amount of conductive pollution

present in the operating environment. Pollution degree 2 assumes that
normally only nonconductive pollution, such as dust, occurs with the
exception of occasional conductivity caused by condensation. ▲

In conformity with the requirements

Chapter 7
Frequently Asked Questions
Q&As Q1: What does the KingFisher Flex system do?
A1: Thermo Fisher Scientific now offers a complete KingFisher Flex
system – the magnetic particle processor – for the purification and
processing of proteins, DNA, RNA and cells in a 24 or 96-well format.
The processor handles particles automatically according to the
preloaded purification protocols. For more information on these
applications, contact Thermo Fisher Scientific Oy.
Q2: What plates can be used with the KingFisher Flex?

A2: The KingFisher Flex is compatible with four different plate types:
KingFisher 24 deep well plates, Microtiter deep well 96 plates,
KingFisher 96 plates, and PCR plates. Each plate type has an optimized
KingFisher Flex head and tips. Of the four plates, the KingFisher 96
plate can be used with all three kinds of magnets (for example,
KingFisher 96 plates can be used together in a protocol with either
Microtiter deep well 96 plates or PCR plates).
Q3: Can I use plates from other manufacturers?

A3: No, it is highly recommended to use plates listed in Table 9–11 on
page 83. These plates are specifically designed to be used with
KingFisher Flex tip combs to attain maximal performance. Plates from
other manufacturers may not be compatible with the KingFisher Flex
heating blocks. They may also cause unexpected problems, such as
cross-contamination, due to the divergent well volume and bottom
height of the plate.
Q4: Will the magnet get weaker? If so, how long can they be used?
A4: The KingFisher Flex magnets are made of material that is very
stable. The magnetic field will not get weaker. However, extreme
mechanical force or heating can cause damage to the magnets.
Caution It is very important to keep the KingFisher Flex heads away

from each other and other magnets at all times. Clashing of the magnets
together may cause serious damage to the magnets. ▲

Q&As
Q5: How strong are the magnets? Can they, for example, disturb some
sensitive equipment?
A5: The KingFisher Flex should not be kept in close proximity to
may cause damage to the display. Do not use metal tools when
handling KingFisher Flex heads.

Q6: How can the KingFisher Flex heads be changed?

A6: By running the Change_magnet protocol under the Maintenance
menu using the up and down cursor keys.
Q7: Can I concentrate the sample during the run?

A7: Both deep well plates and KingFisher 96 plates can be used during
the same run. Therefore, it is possible to start the processing by using
larger volumes (in a deep well plate) and elute the purified sample to a
smaller volume (in a KingFisher 96 plate).
Q8: How does the heating block work?

A8: The heating block is located inside the instrument and can be used
automatically during the protocol. All KingFisher Flex plates can be
heated using specially designed, interchangeable heating blocks. Any
number of heating steps can be added to the protocol. During the
protocol, when the protocol enters the heating step, the plate is
automatically moved to the dedicated heating position for heating.
After the heating step the protocol continues automatically to the next
step.
Note No cooling steps can be added. ▲
Q9: How long does it take the heater to warm up from RT to 80°C?
A9: The heating block warms up about 10 degrees per minute, so it will
take about 6 minutes.

Q&As
Q10: What should I do if the magnets set are dirty?

A10: Wipe the magnetic rods with a soft cloth or tissue paper soaked in
a mild detergent solution, soap solution or alcohol.
Q11: What should I do if I forget to insert the tip combs into the tip comb
holder?
A11: The protocol will not start without the tip combs inserted into the
tip comb holder.
Q12: What if the magnetic particles remained in the sample well?

A12: If the starting material is too viscose, the magnetic rods will not be
able to collect the particles. Dilute the sample and check that the
sample is properly homogenized/lysed.
Adding low amounts of detergent will improve the collection of
magnetic particles as well.
Quickly centrifuge the plate to sediment the particles to the bottom of
the plate.
Q13: What if the magnetic particles are attached to the tip combs after the
run?
A13: This happens sometimes but it will not affect the yield because the
sample has been released from the particles.
Q14: Are the volumes of reagents in each well critical?

A14: It is strongly recommended that you keep the specified volumes
within the defined limits to avoid spillover in the performance of the
chemical reactions and the processor and to keep the best performance
at the most efficient level.
Q15: Is it always compulsory to use the transport locks?

A15: The transport locks are only necessary when relocating the
instrument.
Q16: What should you do if the tip loading does not succeed?
A16: Try first to manually stretch the tips both lengthwise and width
wise to level the tip comb.

Q&As

Chapter 8
Troubleshooting Guide
Note Do not use the instrument if it appears that it does not function
properly. ▲
Note that the instrument does not verify the logic flow of the received
commands.
Error messages When an error is detected, the current operation is terminated. After an
error, it is best to abort the current run and restart from the beginning
and warnings after the problem is fixed. The KingFisher Flex internal software has the
following error messages and warnings (Table 8–8).
Table 8–8. Error messages reported
Code Error message Description
0 No error –
1 The command was not recognized as The command was not recognized as a valid KingFisher Flex command.
a valid command
2 The tip comb holder lifting The magnetic head position is in error.
mechanism is out of position
3 The turntable rotating mechanism is The turntable position is in error.
out of position
4 The magnetic head holder lifting The magnetic tips lift position is in error.
mechanism is out of position
5 The heating block lifting mechanism The heater lift position is in error.
is out of position
6 The shield plate turning mechanism is The shield plate position is in error.
out of position
7 Serial number already set Attempt to set the serial number when it is already set.
8 Invalid command parameter Invalid command argument.
9 Permanent parameters lost Nonvolatile parameters lost.
10 Protocol name already used in other Cannot record a protocol with the same name to another directory.
directory
11 Internal software error Internal software error. This error is actually never reported because
the firmware halts on detection of an internal error. However, this
error may show up in the error log.
12 The requested movement is not Cannot perform the requested movement. For example, the head and
Continued

Troubleshooting guide
Cont.
Code Error message Description
allowed the magnets cannot move down when the shield plate is in place, or
the turntable cannot rotate when the heater is up.
13 The plastic tip comb is not attached No plastic tips attached to the head. The range of movement of the
to the holder head and the magnets is limited if the tips are not attached to the
head. This is to prevent bringing the magnets in direct contact with the
magnetic particle solution.
14 The magnetic head is not inserted to The magnets are missing. The range of movement of the head and the
holder magnets is limited if the magnets are not attached to the magnet lift.
This is to prevent running a purification sequence without the
magnets.
15 The command is not recordable The command is not recordable. This error is reported if a
nonrecordable command is received when recording is on.
16 Not enough memory available for Not enough memory available for operation. For example, maximum
recording operation exceeded in recording.
Code Warning Description
100 Timer expired Timer already expired. This warning is reported if a wait for a timer
(WAI) command is executed and the timer has already expired.
Troubleshooting A troubleshooting guide for the KingFisher Flex instrument is presented

in Table 8–9.
guide
Table 8–9. Actions taken against error messages and warnings
Code Error message Action
0 No error –
1 The command was not recognized Contact authorized technical service.
as a valid command
2 The tip comb holder lifting Switch the instrument OFF and ON, and try again. If the error appears
mechanism is out of position during initialization or is otherwise repeated, contact service.
3 The turntable rotating mechanism Switch the instrument OFF and ON, and try again. If the error appears
is out of position during initialization or is otherwise repeated, contact service.
4 The magnetic head holder lifting Switch the instrument OFF and ON, and try again. If the error appears
5 The heating block lifting Switch the instrument OFF and ON, and try again. If the error appears
6 The shield plate turning Switch the instrument OFF and ON, and try again. If the error appears
7 Serial number already set Contact authorized technical service.
8 Invalid command parameter Contact authorized technical service.
9 Permanent parameters lost Contact authorized technical service.
10 Protocol name already used in Either record to the directory where the protocol is or delete the original
other directory protocol before recording this new one.
11 Internal software error Contact authorized technical service.
Continued

Service request protocol
Cont.
12 The requested movement is not Press the STOP button twice.

allowed
13 The plastic tip comb is not Check if the tips are present. If it looks all right, turn ON and OFF, and run
attached to the holder the check protocol according to the KingFisher Flex head and plastics you
are using (see “Operational check” on page 34).
14 The magnetic head is not inserted Check that the magnets are fully inserted.
to holder
15 The command is not recordable Contact authorized technical service.
16 Not enough memory available for Delete some unnecessary protocols from the internal memory with BindIt
recording operation Software and try again. Repeat this procedure until the protocol fits. It is
recommended that you clean up the program memory from unnecessary
protocols about once a month to prevent this error from occurring.
Code Warning Description
100 Timer expired Contact authorized technical service.
Service request If the KingFisher Flex requires service, contact your local Thermo
Fisher Scientific representative or the Thermo Fisher Scientific technical
protocol service department. Do not under any circumstances send the
instrument for service without any prior contact. It is imperative to
indicate the fault and nature of the required service. This will ensure a
faster return of the instrument to the customer.
Your local Thermo Fisher Scientific representative or distributor will
take care of sending a complaint form (that is, the Warranty Claim
Technical Sheet) to the Thermo Fisher Scientific technical service
department. The Warranty Claim Technical Sheet contains a more
detailed description of the fault, symptom or condition. Give all the
necessary information to the distributor, who will fill out and forward
the Warranty Claim Technical Sheet to the Thermo Fisher Scientific
technical service department.
Check “Packing for service” on page 67. You will find instructions on
how to proceed before shipping the instrument for service to Thermo
Fisher Scientific Oy.
Check that any necessary decontamination procedure has been carried
out before packing. Refer to “Decontamination procedure” on page 66
and “Certificate of Decontamination” on page 82. Ensure that the
Certificate of Decontamination (see Appendix A: “Certificate of
Decontamination”) as well as the return authorization number (RGA)
are sent with the instrument.
The Thermo Fisher Scientific technical service department will keep
you up to date with the progress of service and provide you with any
further details you might need, for example, on maintenance,
serviceability, troubleshooting and replacement.

Certificate of Decontamination
Certificate of The decontamination procedure is required before shipping the

instrument to Thermo Fisher Scientific Oy, for example, for repair. If,
Decontamination for any reason, the instrument is shipped back to Thermo Fisher
Scientific Oy, it must be accompanied by a dated and signed Certificate
of Decontamination, which must be both enclosed and attached to the
outside of the package containing the instrument. Refer to Appendix A:
“Certificate of Decontamination” and “Decontamination procedure”
on page 66.
Failure to confirm decontamination will incur additional labor charges
or at worst the items will be returned for proper cleaning. Before
returning any instrument(s) or item(s), ensure that they are fully
decontaminated. Confirm A or B status. Refer to Appendix A:
“Certificate of Decontamination”.

Chapter 9
Ordering Information
Contact your local Thermo Fisher Scientific representative for ordering
and service information. Ordering information codes are presented in
Table 9–10 through Table 9–12.
KingFisher Flex Table 9–10. Codes for products

Code Instrument / System
5400610 KingFisher Flex – 96 PCR head
5400620 KingFisher Flex – 96 KF head
5400630 KingFisher Flex – 96 deep well head
5400640 KingFisher Flex – 24 deep well plate
Table 9–11. Codes for accessories and consumables

List of
Code Item Quantity
accessories and
N07669 KingFisher Flex User Manual 1
consumables N07974 BindIt Software User Manual 1
5189010 BindIt Software package 1
24074411 KingFisher Flex 96 PCR head and heating block 1
24074421 KingFisher Flex 96 KF head and heating block 1
24074431 KingFisher Flex 96 deep well head and deep 1
well and KF heating blocks
24074441 KingFisher Flex 24 deep well head and heating 1
block
24074410 KingFisher Flex 96 PCR head 1
24074420 KingFisher Flex KF head 1
24074430 KingFisher Flex 96 deep well head 1
24074440 KingFisher Flex 24 deep well head 1
24075410 KingFisher Flex 96 PCR heating block 1
24075420 KingFisher Flex 96 KF heating block 1
24075430 KingFisher Flex 96 deep well heating block 1
24075440 KingFisher Flex 24 deep well heating block 1
97002514 KingFisher Flex 96 tip comb for PCR magnets 80 pcs
Continued

Ordering Information
List of spare parts
Cont.
Code Item Quantity
97002524 KingFisher Flex 96 tip comb for KF 96 magnets 100 pcs
97002534 KingFisher Flex 96 tip comb for DW magnets 100 pcs
97002610 KingFisher Flex 24 deep well tip comb and 50 pcs of each
plate
97002540 KingFisher 96 KF plate (200 µl) 48 pcs
95040450 Microtiter deep well 96 plate, V-bottom 50 pcs
95040460 Microtiter deep well 96 plate, V-bottom, 50 pcs
sterile
95040470 KingFisher Flex 24 deep well plate 50 pcs
95040480 KingFisher Flex 24 deep well plate sterile 50 pcs
List of spare Table 9–12. Codes for spare parts

parts Code Item Quantity
2305290 Serial cable F9/F25 (for RS-232C port) 1
N04001 USB A-B device cable 1.8 m* 1
* Longer USB cables available from PC stores

Chapter 10
References
Keywords for bead-based microspheres
web pages beads nucleic acids

biopanning oligo-dT
cell enrichment particles
cell purification PCR product
cell separation phage display
DNA post PCR purification
DNA extraction primer
DNA immobilization processing
DNA isolation processor
DNA purification protein purification
DNA strand separation proteomics
DNA template purification
double-stranded DNA RNA
genomics DNA mRNA
genomics recombinant protein purification
GST-tag proteins RT-PCR
His-tag proteins separation
immunomagnetic separation silica-coated particles
immunoprecipitation streptavidin-coated beads
KingFisher streptavidin-coated particles
magnetic beads superparamagnetic beads
magnetic capture superparamagnetic microspheres
magnetic microspheres Thermo Fisher Scientific
magnetic particles Thermo Scientific
magnetic separation

References
Literature
Literature Aasheim, H.-C., Deggerdal, A., Smeland, E. B. and Hornes, E. (1994):

A simple subtraction method for the isolation of cell-specific genes
using magnetic mono-disperse polymer particles. Biotechniques 16 (4),
716–721.
Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-
van Dillen, P. M. E. and van der Noordaa, J. (1990): Rapid and simple
method for purification of nucleic acid. J. Clin. Microbiol., 495–503.
Boom, W. R., Adriaanse, H. H., Kievits, T. and Lens, P. F. (1993):
Process for isolating nucleic acids. United States Patent, number:
5,234,809.
Che, S. and Ginsberg, S. D. (2004): Amplification of RNA transcripts
using terminal continuation. Laboratory Investigation 84, 131–137.
Coche, T., Dewez, M. and Beckers, M.-C. (1994): Generation of an
unlimited supply of subtracted probe using magnetic beads and PCR.
Nucleic Acids Res. 22, 1322–1323.
Coty, C. (2002): Purifying Nucleic Acids. Genomics & Proteomics 2 (1),
35–38.
Cudjoe, K. S. (1999): Immunomagnetic particle based techniques:
Overview. In: Eds.: Robinson, R. K., Batt, C. A. and Patel, P. D.
Encyclopaedia of Food Microbiology. Academic Press, London, UK.
Pp. 1088–1094.
Ginsberg, S. D. (2002): High quality mRNA extraction with
KingFisher for molecular fingerprinting. Focus 1, 5.
Ginsberg, S. D. and Che, S. (2002): RNA Amplification in Brain
Tissues. Neurochem. Res. 27 (10), 981–992.
Hawkins, D. J., O’Connor-Morin, T., Roy, A. and Santillan, C.
(1994): DNA purification and isolation using a solid-phase. Nucleic
Acids Res. 22, 4543–4544.
Hornes, E. and Korsnes, L. (1990): Magnetic DNA hybridisation
properties of oligonucleotide probes attached to superparamagnetic
beads and their use in the isolation of poly(A) mRNA from eukaryotic
cells. Genet. Anal. Tech. Appl. (GATA) 7, 145–150.
Jakobsen, K. S., Breivold, E. and Hornes, E. (1990): Purification of
mRNA directly from crude plant tissues in 15 minutes using oligo dT
microspheres. Nucleic Acids Res. 18, 3669.
Jakobsen, K. S., Haugen, M., Sæboe-Larsen, S., Hollung, K., Espelund,
M. and Hornes, E. (1994): Direct mRNA isolation using magnetic
Oligo (dT) Beads: A protocol for all types of cell cultures, animal and
plant tissues. In: Eds.: Uhlén, M., Hornes, E. and Olsvik, Ø. Advances
in Biomagnetic Separation. Eaton Publishing. Pp. 61–71.

References
Josefsen, M. H., Krause, M., Hansen, F. and Hoorfar, J. (2007):

Optimization of a 12-hour TaqMan PCR-based method for detection
of salmonella bacteria in meat. Applied and Environmental Microbiology
73, 3040–3048.
Kim, I.-J. et al. (2003): Development and Applications of a β-Catenin
Oligonucleotide Microarray: β-Catenin Mutations Are Dominantly
Found in the Proximal Colon Cancers with Microsatellite Instability.
Clin. Cancer Res. 9, 2920–2925.
Konthur, Z. and Walter, G. (2002): Automation of phage display for
high-throughput antibody development. TARGETS 1 (1), 30–36.
Lambert, K. N. and Williamson, V. M. (1993): DNA library
construction from small amounts of RNA using paramagnetic beads
and PCR. Nucleic Acids Res. 21, 775–776.
Lee, Y.-H., and Vacquier, V. D. (1992): Reusable cDNA libraries
coupled to magnetic beads. Anal. Biochem. 206, 206–207.
Lund, M., Wedderkopp, A., Wainø, M., Nordentoft, S., Bang, D. D.,
Pedersen, K. and Madsen, M. (2003): Evaluation of PCR for detection
of Campylobacter in a national broiler surveillance programme in
Denmark. J. Appl. Microbiol. 94, 929–935.
Mäkinen, J., Marttila, H. and Viljanen, M. K. (2001): Automated
purification of Borrelia burgdorferi s.1. PCR products with KingFisher
magnetic particle processor prior to genome sequencing. Journal of
Magnetism and Magnetic Materials 225, 134–137.
Portelius, E., Tran, A. J., Andreasson, U., Persson, R., Brinkmalm, G.,
Zetterberg, H., Blennow, K. and Westman-Brinkmalm, A. (2007):
Characterization of amyloid β peptides in cerebrospinal fluid by an
automated immunoprecipitation procedure followed by mass
spectrometry. Journal of Proteome Research 6, 4433-4439.
Robino, C., Barilaro, M. B., Gino, S., Chiarle, R., Palestro, G. and
Torre C. (2006): Incestuous paternity detected by STR-typing of
chorionic villi isolated from archival formalin-fixed paraffin-embedded
abortion material using laser microdissection. Journal of Forensic Sciences
51, 90–92.
Rudi, K., Kroken, M., Dahlberg, O. J., Deggerdal, A., Jakobsen, K. S.
and Larsen, F. (1997): Rapid, universal method to isolate PCR-ready
DNA using magnetic beads. BioTechniques 22, 506–511.
Sinclair, B. (1998): To bead or not to bead. Applications of magnetic
bead technology. Scientist 12, 16–19.
Tu, S., Golden, M., Cooke, P., Paoli, G. and Gehring, A. (2005):
Detection of Escherichia coli O157:H7 through the formation of
sandwiched complexes with immunomagnetic and fluorescent beads.
Journal of Rapid Methods and Automation in Microbiology 13, 269–282.

References
Literature
Valenzuela, D. M. et al. (2003): High-throughput engineering of the

mouse genome coupled with high-resolution expression analysis. Nature
Biotechnology 21 (6), 652–659.
Wan, L., Battle, D.J., Yong, J., Gubitz, A. K., Kolb, S. J., Wang, J. and
Dreyfuss, G. (2005): The survival of motor neurons protein determines
the capacity for snRNP assembly: Biochemical deficiency in spinal
muscular atrophy. Molecular and Cellular Biology 25, 5543–5551.
Ward, L. I., Fenn, M. G. E. and Henry, C. M. (2004): A rapid method
for direct detection of Polymyxa DNA in soil. Plant Pathology 53,
485–490.
Ye, J.-D., Tereshko,V., Frederiksen, J. K., Koide, A., Fellouse, F. A.,
Sidhu, S. S., Koide, S., Kossiakoff, A. A. and Piccirilli, J. A. (2008):
Synthetic antibodies for specific recognition and crystallization of
structured RNA. PNAS 105, 82–87.

Appendix A
Name:
Address:
Tel./Fax:
Name: Serial no.:
A) I confirm that the returned items have not been contaminated by body fluids, toxic,
carcinogenic or radioactive materials or any other hazardous materials.
B) I confirm that the returned items have been decontaminated and can be handled without
exposing the personnel to health hazards.
Materials used in the unit: Chemicals + Biological • Radioactive *)
Specific information about contaminants:
Decontamination procedure1:
Date and place:

Signature:
Name (block capitals):
*) The signature of a Radiation Safety Officer is also required when the unit has been used with
radioactive materials.
This unit is certified by the undersigned to be free of radioactive contamination.
Date and place:

Signature:
Name (block capitals):
PHOTOCOPIABLE
1
Please include decontaminating solution used.


Appendix B
Thermo Scientific KingFisher Flex
Feedback Form
Instrument: Thermo Scientific KingFisher Flex Instrument serial no.:
PURCHASED BY PURCHASED FROM

Company/Institute Distributor
Department Address
Address
Tel. Tel.
Fax Date of delivery
Internet home page
Date of purchase
Your application area
Dr. Mr. Mrs. Ms. Job title/Position
Surname (block capitals) First name (block capitals)
Internet e-mail address

Excellent Above expectations As expected Below expectations Comments
Instrument installation
Ease of use
Flexibility
User manual
Software
Customer support
Overall
Additional instrument and/or software features desired:
Did you encounter any problems?
Where did you first learn about the product?
Would you like to receive information about other Thermo Scientific products?

Thermo Scientific KingFisher Flex Feedback Form

Glossary
heating block There are four different kinds of tip comb Protects the magnetic rods (24 or 96 in a
interchangeable heating blocks available, that is, frame). A disposable tip comb always has to be
for KingFisher 24 deep well plates, Microtiter placed onto the microplate prior to processing.
deep well 96 plates, KingFisher 96 plates, and There are four different kinds of disposable tip
PCR plates (Figure 4–48). combs available:
KingFisher Flex head There are four kinds of • for KingFisher 24 deep well plates
interchangeable KingFisher Flex heads available,
• for Microtiter deep well 96 plates
for KingFisher 24 deep well plates, Microtiter
deep well 96 plates, KingFisher 96 plates, and • for KingFisher 96 plates
PCR plates. The KingFisher Flex heads all have
• for PCR plates
corresponding disposable plastic tip combs.
turntable A turntable with eight plate stations that
magnetic bead (magnetic particle) The magnetic
can be detached for cleaning purposes
particles attach to the magnetic rods
(Figure 2–2).
(Figure 2–7 and Figure 2–8) that are protected
by a disposable tip comb. The magnetic
particles enable the purification of a variety of
target molecules.
magnetic rod The rods which are magnetic and
collect magnetic particles (Figure 4–43). The
rods do not collect the particles on their own,
the magnetic rods must always be protected by a
tip comb.
plate The disposable plates (1 – 8) where all the
reagents and samples are located and where the
processing takes place. Four different types of
24 and 96-well plates (Figure 4–45) that can be
used are as follows:
• KingFisher 24 deep well plate
(200 µl – 5 mL*)
• Microtiter deep well 96 plate
(50 – 1000 µl*)
• KingFisher 96 plate (20 – 200 µl*)
• PCR plate (20 – 100 µl*), skirted
shield plate When the turntable moves, the shield
plate moves over the plate underneath
(Figure 4–44) forming a protective cover.

Glossary:

Index
error message, 79, 80

A
accessories, 21, 22, 83 F
application, 2, 5, 91 factory protocols, 41, 45, 47
B feedback form, 21, 91

BindIt Software, 3, 13, 14, 21, 39, 43, 45, 55, 81, 83
buzzer, 44, 50, 51 H
handling tip combs, 52
C heating block, 23, 25, 26, 27, 28, 29, 35, 36, 38, 59, 60, 61,
Cell, 43, 47 72, 76, 79, 80, 83, 93
Certificate of Decontamination, 67, 68, 81, 82, 89
changing the heating block, 59 I
clean, 22, 63, 65, 67, 81 icon, 42, 43
magnetic rods, 65 incubation, 18
shield plate, 66 installation, 3, 21, 22, 23, 27, 29, 67, 73, 91
turntable, 63 installing the KingFisher Flex, 21, 23, 73
cleaning the magnetic rods, 65 instrument layout, 15
cleaning the shield plate, 66 instrument options, 48
cleaning the turntable, 63 intended use, 3, 13
computer interface, 44, 49, 71 inverse magnetic particle processing, 17
concentration, 14, 18, 19
control panel, 40 K
keyboard, 13, 40, 72
D keys, 27, 33, 40, 41, 42, 45, 46, 47, 48, 49, 50, 51, 52, 55,
decontaminating the instrument, 61, 66, 68, 81, 82, 89 59, 72, 76
decontamination, 61, 66, 67, 68, 81, 82, 89 keywords for web pages, 85
decontamination procedure, 61, 66, 67, 68, 81, 82, 89
default factory protocol name, 43, 45, 46, 47 L
default user protocol name, 43, 45, 46, 47 language, 44, 50
deep well plate, 28, 29, 31, 32, 34, 54, 59, 61, 72, 75, 76, literature, 86
83, 93
loading position, 56
device information, 43, 48
display, 13, 16, 23, 32, 34, 40, 41, 63, 65, 72, 75, 85, 87
M
disposal of instrument, 68
disposal of materials, 8, 66 magnetic particles, 13, 14, 17, 18, 77, 85, 93
DNA/RNA, 43, 47 collection of, 18, 77
release of, 18
wash of, 18
E
magnetic rod, 2, 13, 16, 17, 18, 33, 59, 65, 72, 76, 77, 93
ensuring startup, 26

maintain, 63, 68 see-through lid, 6, 15, 17, 27, 34, 39, 55, 58, 59, 67
maintaining the instrument, 3, 27, 33, 35, 41, 44, 48, 49, service, 39, 62, 66, 67, 68, 80, 81, 83
50, 51, 63, 76 contracts, 68
maintenance, 3, 6, 27, 33, 35, 41, 44, 48, 49, 50, 51, 52, 63, request protocol, 81
68, 76, 81
shield plate, 27, 29, 30, 31, 58, 63, 66, 79, 80, 93
protocol, 44, 51, 52
shutting down, 61
sliding door, 6, 17, 34, 39, 55, 58, 59
N specifications, 3, 13, 71
navigating, 41 general, 68, 71
performance, 71
O safety, 68, 72, 73
operational check, 34, 39, 81 switching on, 40, 59
ordering information, 3, 83 symbols, 4
Other, 43, 47
T
P tip comb, 13, 16, 17, 18, 23, 24, 25, 31, 32, 33, 35, 36, 37,
38, 39, 52, 53, 54, 55, 56, 57, 58, 59, 61, 72, 76, 77, 79,
packing, 21, 22, 67, 69, 81 80, 81, 83, 84, 93
instructions, 21, 67 holder, 23, 24, 25, 33, 35, 36, 37, 38, 57, 58, 76, 79, 80
list, 21, 22 transport, 21, 22, 23, 24, 25, 26, 35, 36, 37, 38, 67, 68, 71,
materials, 69 77
PCR, 28, 29, 31, 32, 53, 54, 59, 61, 72, 75, 83, 85, 86, 87, damage, 22
93 locks, 23, 24, 25, 26, 35, 36, 37, 38, 67, 77
plate, 28, 29, 31, 32, 59, 61, 72, 75, 93 package, 21, 22, 67, 71
plate, 6, 13, 15, 16, 17, 18, 28, 29, 30, 31, 32, 39, 40, 52, troubleshooting, 3, 79, 80, 81
53, 54, 55, 56, 57, 58, 59, 60, 61, 66, 72, 75, 76, 77, 84,
93 guide, 80
station, 16, 55, 56, 93 the instrument, 80
processing head, 16
Protein, 43, 47 U
protocol, 13, 16, 27, 28, 33, 35, 40, 43, 45, 46, 47, 48, 51, unpacking, 21, 52, 54
52, 55, 56, 59, 60, 75, 76, 79, 80, 81, 86 user protocols, 41, 45, 47
using BindIt Software, 45
R using internal software, 45
refitting the transport lock of the heating block, 26, 35
refitting the transport lock of the tip comb holder, 25, 26, 36 W
refitting the transport locks, 25, 26, 35, 36 warnings, 3, 4, 5, 6, 21, 23, 27, 29, 33, 40, 61, 63, 65, 66,
68, 76, 80, 81
S warranty, 2, 21, 22, 39, 81
samples, 13, 14, 16, 23, 59, 66, 72, 76, 77, 93 working with a magnetic rod, 18

Notes

Notes

Notes

Thermo Fisher Scientific Oy
Ratastie 2, P.O. Box 100
FI-01621 Vantaa
Finland
www.thermoscientific.com
N07669

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Rev. 1.2, Cat. no. N07669

No liability for consequential damages

Consumables are not included in the warranty.

In our efforts to provide useful and appropriate documentation, we

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 3

Warning Hot surface, risk of burns. ▲

Warning Risk of body parts, hair, jewelry or clothing getting caught in

Consult instructions for use ▲

WEEE symbol This product is required to comply with the European

A black label with the following text (Figure 2–4):

4 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Warning Risk of electric shock. ▲

Warning Biohazard risk. ▲

Warning Risk of injury to the user(s). ▲

Caution Risk of damage to the instrument, other equipment or loss of

Note Marks a hint, important information that is useful in the

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 5

Warning This product contains very strong permanent magnets.

6 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Chapter 1 Introduction to the KingFisher® Flex........................................................ 13

Chapter 2 Functional Description ............................................................................... 15

Chapter 3 Installation .................................................................................................... 21

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 7

How to refit the transport lock of the tip comb holder................. 36

Chapter 4 Routine Operation ........................................................................................39

Chapter 5 Maintenance .................................................................................................63

Chapter 6 Technical Specifications ...........................................................................71

Chapter 7 Frequently Asked Questions ......................................................................75

8 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Chapter 8 Troubleshooting Guide ............................................................................... 79

Chapter 9 Ordering Information................................................................................... 83

Chapter 10 References .................................................................................................... 85

Appendix A Certificate of Decontamination................................................................. 89

Appendix B Thermo Scientific KingFisher Flex Feedback Form.............................. 91

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 9

10 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Figure 4–37. 24 deep well tip comb package ............................................... 53

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 11

12 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Principle of The KingFisher Flex magnetic particle processor (Figure 1–1) is

Figure 1–1. KingFisher Flex magnetic particle processor

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 13

14 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Heating block Shield plate Plate station (1 – 8)

Figure 2–2. KingFisher Flex front view without see-through lid

Sliding door (open)

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 15

Serial port RS-232C ON/OFF switch

Figure 2–4. KingFisher Flex back view

Figure 2–5. KingFisher Flex side view

16 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

During the operation, the sliding door can be closed or open

Figure 2–6. KingFisher Flex magnetic particle processor

Figure 2–7. Inverse magnetic particle processing

Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 17

Washing magnetic Washing the magnetic particles is a frequent and an important

Incubation To keep the magnetic particle suspension evenly mixed in long-running

18 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific

Figure 2–8. A concentration step during magnetic particle processing