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Experiment 8

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Anuj Gupta
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119 views3 pages

Experiment 8

Uploaded by

Anuj Gupta
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF or read online on Scribd
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(of Portulaca/grass ot any other suitable flower, glass slide, coverslip, ropper, safranin, glycerine, petridish, water, blotting paper, microscope ete pollinated carpel from the lower of Portulaca/grass or any other suitable flower ce iton a glass side in a drop of water. Gently tease it with the help of needles or pick carpel from the flower and cut a longitudinal section of it. Place the section on a glas de in a drop of water. “4 Artipodal cols a 8 Fig. 10.1. Pollen germination. A. Pollen grains germinating on stigma (a teased preparation). 8. Growth of pollen tube in the carpel (LS). Pour a drop of safranin on the teased carpel or its section and wash it with water. Put a drop of glycerine and cover the teased carpel or its section with coverslip. Remove the extra glycerine with blotting paper. Observe the preparation under the high power of microscope and draw the diagrams of different stages of pollen germination, BSERVATION Different stages of germinating pollens ae observed inthe stigmaand style regions ofthe carpe * pollen ae in their intial stage of germination others have quite longpollen tube containing tbe leus and two male nuclei. RECAUTIONS 1 2 3 Only pollinated carpels should be selected for the experiment. Teasing should be done gently, so that the pollen tubes are not ruptured Excess of glycerine/water should be removed by blotting paper,

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