FAKULTI SAINS GUNAAN

UNIVERSITI TEKNOLOGI MARA

BIO 462: BIOCHEMISTRY

LABORATORY REPORT

NAME: BALQISH NADHIRAH BINTI SAHLAN

STUDENT ID: 2022470524
GROUP: AS257_2B
PREPARED DR. LYENA WATTY ZURAINE AHMAD
FOR:
EXPERIMENT 1: Dilution and Molarity

Objectives

1. To understand the calculation for setting up the dilution.

2. To know to do the dilution optimally.
3. To understand the calculation for final dilution.

Post-lab questions

I. How would you prepare

a. 10 ml of a 1:10 dilution of a 1M NaCl solution and what would the final
concentration of NaCl?

M1 = 1 M V1 =?
M2 = 1:10 × 1 V2 = 1 ml
1
= ×1
10
= 0.1 M

M1VI = M2V2
(1M)(V1) = (0.1M)(10 ml)
V1= 1 ml

Mix 1 ml of 1 M NaCl with 9 ml of solvent.

b. 80 ml of a 1:20 dilution of a 1M NaCl solution?

M1 = 1 M V1 =?
M2 = 1:20 × 1 M V2 = 80 ml
1
= ×1
20
= 0.05 M

M1VI = M2V2
(1 M)(V1) = (0.05M)(80 ml)
V1 = 4 ml

Mix 4 ml of 1 M NaCl with 76 ml of solvent.

c. 50 ml of a 1:25 dilution of a 1 M NaCl solution?

M1 = 1 M V1 =?
M2 = 1:25 × 1 M V2 = 50 ml
 1
= ×1M
25
= 0.04 M

M1VI = M2V2
(1 M)(V1) = (0.04 M)(50ml)
V1 = 2 ml

Mix 2 ml of 1 M NaCl with 48 ml of solvent.

II. How would you prepare exactly 6 ml of a 1/20 dilution (assume the concentration
of your starting solution is “1”)?

M1 = 1 M V1 =?
M2 = 1:20 × 1 M V2 = 6 ml
1
= ×1M
20
= 0.05 M

M1VI = M2V2

(1 M)(V1) = (0.05 M)(6 ml)

V1 = 0.3 ml

Mix 0.3 ml of stock solution with 5.7 ml of solvent.

III. You are provided with an antibody solution (Ab) that has a concentration of 600
microgram (μg) / microlitre (μL). For lab, it is necessary to make the following
dilutions:

a. 10 μL of 600 μg / μL Ab + 190 μL of buffer to make a 1:20 dilution at 30

μg / μL.

M1 = 600 μg / μL V1 = 10 μL
M2 =? V2 = 190 μL + 10 μL
= 200 μL

M1VI = M2V2
(600)(10) = (200)(M2)
200 M2 = 6000
M2 = 30 μg / μL

b. 20 μL of 1:20 Ab + 40 μL of buffer to make a 1:60 dilution at 10 μg / μL.

M1 = 1:20 V1 = 20 μL
 1
= × 600 V2 = 40 μL + 20 μL
20
= 30 μg / μL = 60 μL
M2 =?

M1VI = M2V2
(30)(20) = (60)(M2)
60 M2 = 600
M2 = 10 μg / μL
c. 5 μL of 1:60 Ab + 5 μL of buffer to make a 1:120 dilution at 5 μg / μL.

M1 = 1:60 V1 = 5 μL
1
= × 600 V2 = 5 μL + 5 μL
60
= 10 μg / μL = 10 μL
M2 =?

M1VI = M2V2
(10)(5) = (10)(M2)
10 M2 = 50
M2 = 5 μg / μL

5
Dilution factor =
600
1
=
120
= 1:120

d. 10 μL of 1:60 Ab + 90 μL of buffer to make a 1:600 dilution at 1 μg / μL.

M1 = 1:60 V1 = 10 μL
1
= × 600 V2 = 10 μL + 90 μL
60
= 10 μg / μL = 100 μL
M2 =?

M1VI = M2V2
(10)(10) = (100)(M2)
100 M2 = 100
M2 = 1 μg / μL

1
Dilution factor =
600
= 1:600
 e. 10 μL of 1:60 Ab + 40 μL of buffer to make a 1:300 dilution at 2 μg / μL.

M1 = 1:60 V1 = 10 μL
1
= × 600 V2 = 40 μL + 10 μL
60
= 10 μg / μL = 50 μL
M2 =?

M1VI = M2V2
(10)(10) = (50)(M2)
50 M2 = 100
M2 = 2μg / μL

2
Dilution factor =
600
1
=
300
= 1:300

f. 10 μL of 1:60 Ab + 10 μL of buffer to make a 1:120 dilution at 5 μg / μL.

M1 = 1:60 V1 = 10 μL
1
= × 600 V2 = 10 μL + 10 μL
60
= 10 μg / μL = 20 μL
M2 =?

M1VI = M2V2
(10)(10) = (20)(M2)
20 M2 = 100
M2 = 5 μg / μL

5
Dilution factor =
600
1
=
120
= 1:120

IV. How much 2.0 M NaCl solution would you need to make 250 ml of 0.15 M NaCl
solution?

M1 = 0.15 M V1 = 250 ml
M2 = 2.0 M V2 =?
 M1VI = M2V2
(0.15)(250) = (2.0) (V2)
2V2= 37.5
V2 = 18.75 ml

V. What would be the concentration of a solution made by diluting 45.0 ml of 4,2 M

KOH to 250 ml?

M1 = 4.2 M V1 = 45.0 ml
M2 =? V2 = 250 ml

M1VI = M2V2
(4.2)(45.0) = (2.0) (M2)
250M2= 0.756
M2 = 0.8 M

VI. What would be the concentration of a solution made by adding 250 ml of water
to 45.0 ml of 4.2 M KOH?

M1 =? V1 = 250 ml
M2 = 4.2 M V2 = 45.0 ml

M1VI = M2V2
(M1)(250) = (4.2) (45.0)
250M1= 189
M1 = 0.756
M1= 0.8 M

VII. How much 0.20 M glucose solution can be made from 50.0 ml of 0.50 M glucose
solution?

M1 = 0.50 M V1 = 50 ml
M2 = 0.20 M V2 =?

M1VI = M2V2
(0.50)(50.0) = (0.20) (V2)
0.20V2 = 25
V2 = 125 ml

VIII. What is the molarity of a solution that has 4.5 mol of solute dissolved in 300 ml of
solution?
 moles of solute = 4.5 mol
litres of solution = 300 ml

moles of solute
Molarity =
litres of solution
4.5 mol
=
300 ml
= 0.015 mol/ml

IX. What is the molarity of a solution of NaOH that has 0.491 g dissolved in 400 ml
of solution?

moles of solute
Molarity =
litres of solution
mass of NaOH
No. moles of NaOH =
mass of 1mol of NaOH
Mass for 1 mol NaOH = 39.997 g/mol
0.419 g
=
39.997 g /mol
= 0.01228 mol
0 .01228mol
Molarity of NaOH solution =
0.4 l
= 0.0307 M

X. What is the molarity of a solution prepared by diluting 10.00 ml 0f 4.281 M

solution 50.00 ml?

M1 = 4.281 M V1 = 10 ml
M2 =? V2 = 50 ml

M1VI = M2V2
(4.281)(10) = (M2) (50)
50 M2= 42.81
M2 = 0.8562 M

References

SUHAIMI, M. B. (2020). studocu. Retrieved from Lab Report 1 BIO462 Biochemistry :

Dilution & Molarity: https://www.studocu.com/my/document/universiti-teknologi-
malaysia/biochemistry/lab-report-1-bio462-biochemistry-dilution-molarity/20789063