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    Blood Culture & Sensitivity (2011734)

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    Najib Aiman
    Blood culture & sensitivity is a laboratory test where blood is inoculated into culture bottles to identify infectious microorganisms in the bloodstream. It is indicated to confirm infections, identify causative agents, and guide antimicrobial therapy for conditions like sepsis, endovascular infections, and localized infections. The proper technique involves preparing culture bottles, selecting a vein site, performing venipuncture aseptically, inoculating blood into aerobic and anaerobic bottles, collecting other blood tests if needed, and sending samples to the laboratory within 2 hours.

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    Blood Culture & Sensitivity (2011734)

    Uploaded by

    Najib Aiman
    93 views11 pages
    Blood culture & sensitivity is a laboratory test where blood is inoculated into culture bottles to identify infectious microorganisms in the bloodstream. It is indicated to confirm infections, identify causative agents, and guide antimicrobial therapy for conditions like sepsis, endovascular infections, and localized infections. The proper technique involves preparing culture bottles, selecting a vein site, performing venipuncture aseptically, inoculating blood into aerobic and anaerobic bottles, collecting other blood tests if needed, and sending samples to the laboratory within 2 hours.

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    Blood culture & sensitivity is a laboratory test where blood is inoculated into culture bottles to identify infectious microorganisms in the bloodstream. It is indicated to confirm infections, identify causative agents, and guide antimicrobial therapy for conditions like sepsis, endovascular infections, and localized infections. The proper technique involves preparing culture bottles, selecting a vein site, performing venipuncture aseptically, inoculating blood into aerobic and anaerobic bottles, collecting other blood tests if needed, and sending samples to the laboratory within 2 hours.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    93 views11 pages

    Blood Culture & Sensitivity (2011734)

    Uploaded by

    Najib Aiman
    Blood culture & sensitivity is a laboratory test where blood is inoculated into culture bottles to identify infectious microorganisms in the bloodstream. It is indicated to confirm infections, identify causative agents, and guide antimicrobial therapy for conditions like sepsis, endovascular infections, and localized infections. The proper technique involves preparing culture bottles, selecting a vein site, performing venipuncture aseptically, inoculating blood into aerobic and anaerobic bottles, collecting other blood tests if needed, and sending samples to the laboratory within 2 hours.

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    © All Rights Reserved
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    Download as pdf or txt
    of 11

    Blood Culture &

    Sensitivity
    Prepared by: Anfal Hannan binti Abdul Aziz (2011734)
    Table of contents

    01 Introduction
    04 Equipmentss

    Indications &
    02 Complications
    05 Technique

    03 Site selection
    Introduction
    Definition:

    A laboratory test in which blood, taken from the patient,


    is inoculated into bottles containing culture media to determine whether
    infection-causing microorganisms (bacteria or fungi) are present in the
    patient’s bloodstream.

    Aim:
    - Confirm infectious aetiology
    - Identify the etiological agent
    - Guide antimicrobial therapy
    Indications Complications
    ● Sepsis ● Hematoma
    - Shock, fever, chills, rigor, tachycardia, ● Fainting
    tachypnea, decrease urine output ● Infection

    ● Endovascular infection
    - Vascular graft, history of IE, prosthetic valve,
    pacemaker, intravascular catheter infection

    ● Local infections
    - Vertebral osteomyelitis, meningitis, epidural
    abscess, septic arthritis, pneumonia

    ● Leukocytosis/ leukopenia
    Site selection
    Preferred site:
    ● Median cubital vein in antecubital
    fossa commonly used for
    venepuncture
    ● Other upper extremity blood vessels

    Prohibited sites:
    ● Veins proximal to existing IV cannula
    ● Areas of broken skin or bruising

    To identify a vein:
    ● Push the vein firmly with index finger
    and slowly release the pressure
    ● The vein will bounce back
    Equipment
    ● Apron
    ● Non-sterile gloves
    ● Tourniquet (single-use)
    ● Blood sampling device with blood
    culture bottle adapter (e.g. winged
    blood collection set)
    ● Blood culture bottles x 2 (anaerobic
    and aerobic):
    ● Sharps container
    ● Cleaning swab x 3 (2% chlorhexidine in
    70% isopropyl alcohol)
    ● Sterile gauze
    ● Sterile plaster
    ● Tape
    ● Laboratory forms, labels and
    transportation bag
    Technique
    Pre-procedure basics: 1. Prepare bottles for inoculation
    ● Greet the patient ● Wash hands with soap
    ● Introduce yourself and water then dry
    ● Explain the procedure ● Remove the plastic flip-cap
    ● Gain consent from the bottles
    ● Confirm patient’s identity ● Disinfect the septum
    ● Gather all required materials before ● Allow bottle tops to dry to fully disinfect
    beginning collection process ● Don gloves and apron

    2. Prepare venipuncture site


    ● Clean with soap and water if skin is
    visibly soiled
    ● Apply a disposable tourniquet
    ● Palpate and identify a vein using clean
    examination gloves
    ● Cleanse puncture area using appropriate
    disinfectant
    ● Allow disinfectant to fully evaporate
    Technique
    3. Venipuncture
    4. Culture bottle inoculation
    ● Attach a winged blood collection set to
    ● Place the adapter cap over aerobic
    a collection adapter cap
    bottle and press straight down to pierce
    ● Do not re-palpate the prepared vein
    the septum
    before inserting the needle
    ● Hold the bottle upright, below the draw
    ● Insert the needle into prepared vein
    site and add up to 10 mL of blood per
    ● At 30૦ angle or less, bevel facing
    adult bottle and up to 4 mL per pediatric
    forwards
    bottle
    ● Observe for flashback of blood
    ● Repeat procedure for anaerobic bottle
    Technique
    5. Other blood tests

    ● If blood is being collected for other tests, an


    insert placed into the adapter cap may be
    required. The insert will guide blood collection
    tubes into the needle.
    ● If other blood tests are requested, always
    collect blood culture first.

    6. Finish the procedure


    ● Release the tourniquet and apply gauze, then
    remove the butterfly needle
    ● Discard winged collection kit into sharps
    container
    ● Remove gloves and perform hand hygiene
    ● Labels the bottle carefully and correctly
    ● Send blood sample to lab (within 2 hours)
    ● Thank the patient
    Video
    References
    ● Taking blood cultures. A summary of best practices. UK Government Web Archive.
    Published in 2011. Retrieved from:
    http://apps.who.int/iris/bitstream/handle/10665/44294/9789241599221_eng.pdf;jsessio
    nid=0919D680ED146E42B9EC430D41D1F620?sequence=1

    ● Blood culture: A key investigation for diagnosis of bloodstream infections. (n.d.).


    Retrieved February 10, 2021, from
    http://go.biomerieux.com/l/149271/2017-09-08/39hb9d

    ● Dr Lewis Potter·Clinical Procedures·Last updated:January 7, 2. (2021, January 07).


    Blood culture collection -osce guide: Venepuncture. Retrieved February 10, 2021, from:
    https://geekymedics.com/blood-culture-collection-osce-guide/

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