Question Bank

Answers of the
questions from
lect 1 to lect6
Questions
2. What is the pH of a solution with
an H+ ion concentration of 10-4
mole/L? pH=4
3. What is the pH of solution with an
H+ ion concentration of 5.0 X 10-6
mole/L?
pH = - log[ H+]
pH=5.4
2
Which of the following cannot form the
inner reference electrode in glass
electrodes?
a) Silver electrode
b) Copper electrode
c) Calomel electrode
d) Silver chloride electrode
3
Questions
1. RCF is measured in which o the ollowing units?
a. Gravities (g)
b. Centimeters (cm)
c. RPMs
d. Forces ( f)
4
Questions
4. Which of the following statements is FALSE?
A. An acid is a proton donor and a base is a proton acceptor.
B. An acidic solution has a pH greater than 7, and a basic solution
has a pH less than 7.
C. Neutralisation of an acid by a base gives a solution of salt in
water.
D. The pH of the stomach is normally in the range of 1.6–1.8.
5
5. What does the pH of a buffered solution depend on?
A. The ratio of the components of the buffer solution
B. The amount of acid added to the buffer solution
C. The amount of base added to the buffer solution
D. The amount of acid and of base added to the solution
6
6. Acidity is stated as a pH value. If the pH of urine sample
“A” is 6 and the pH of urine sample “B” is 7, then which of
the following is true?
• A. The most acidic sample is sample B.

• B. Sample A has ten times the hydroxide ion concentration of sample B.
• C. Sample B has ten times the hydrogen ion concentration of sample A.
• D. Sample A has ten times the hydrogen ion concentration of sample B.
7
7. Which of the following is not the characteristic of a
reference electrode?
• a) It must have a known output potential

b) It must have a constant output potential
c) Its output potential is dependent on the composition of the
solution
d) It is employed in conjunction with the indicator or working
electrod
8
What monitors the temperature inside the incubator?
• A. humidity
• B. air pressure
• C. thermometer
• D. bp cuff
Which of the following techniques has the highest potential sensitivity?
a. Chemiluminescence
b. Fluorescence
c. Turbidimetry
d. Nephelometry
e. Phosphorescence
Question
2. Stray light in a spectrophotometer places limits on
a. Upper range of linearity
b. Sensitivity
c. Photometric accuracy below 0.1 absorbance units
d. Ability to measure in the UV range
e. Use of a grating monochromator
Question
3. What units of measurement are traditionally applied when
wavelengths in the electromagnetic spectrum are
measured?
a. Millimeters (mm)
b. Centimeters (cm)
c. Nanometers (nm)
d. Micrometers (μm)
Question
4. Oxidation of an organic compound with resultant light emission
is known as:
a. nephelometry.
b. turbidimetry.
c. chemiluminescence.
d. fluorescence.
Question
5. The expression of the relationship between the concentration of
a substance in solution and absorbance of light by that substance is
referred to as Beer’s law. This relationship is expressed in a
formula as:
a. log (1/T).
b. C = abc.
c. Io/Is × 100.
d. A = abc.
Question
6. Which component of a single-beam spectrophotometer isolates
radiant energy of a specific wavelength and excludes that of other
wavelengths?
• a. Monochromator
• b. Entrance slit
• c. Cuvet
• d. Light source
Question
7. Measurement of the concentration of a substance that is
performed by measuring the decreased intensity of light blocked by
particles in solution at 180° from the source is called:
a. fluorometry.
b. atomic absorption.
c. turbidimetry.
d. nephelometry.
Question
8. A solution that is identical to that of calibrating or unknown
solutions except that the substance to be measured and that is used
to set 100% (zero absorbance) at the beginning of a photometric
analysis is referred to as a:
a. standard solution.
b. calibrating solution.
c. reference blank.
d. reagent blank.
Question
9. Which of the following is Beer law?
a. A = ε × b × c
b. %T = I/I0 × 100
c. E = hv
d. e = ΔpH × 0.59 V
e. Osmolality = j × n × C
Question
10. Which of the following correctly ranks electromagnetic
radiation from low energy to high energy?
a. Microwaves, infrared, visible, UV, x-rays, gamma, cosmic
b. Cosmic, gamma, x-rays, UV, visible, infrared, microwaves
c. UV, visible, infrared, microwaves, x-rays, cosmic, gamma
d. UV, visible, infrared, cosmic, gamma, microwaves, x-rays
e. Visible, UV, infrared, cosmic, gamma, microwaves, x-rays
Question
11. Which is the most sensitive detector for spectrophotometry?
a. Photomultiplier.
b. Phototube.
c. Electron multiplier.
d. Photodiode array.
e. All are equally sensitive
Questions
• What quality incorporated into a spectrophotometer can
sometimes improve the linearity of a chemistry procedure?
• A. Flow-through cuvette
• B. Wider bandwidth
• C. Narrower bandwidth
• D. Chopper
Questions
• When a pH-sensitive glass electrode is not actively in use, in
what type of solution should it be kept?
• A. Tap water
• B. Physiologic saline solution
• C. The medium recommended by the manufacturer
• D. A buffer solution of alkaline pH
• In spectrophotometry, which of the following is a mathematical
expression of the relationship between absorbance and
transmittance?
• A. A = abc
• B. Au _ As C~~C ^U ^5
• C. A = 2 - log %T
• D. A = log %T
• In spectrophotometric analysis, what is the purpose of the reagent
blank?
• A. Correct for interfering chromogens
• B. Correct for lipemia
• C. Correct for protein
• D. Correct for color contribution of the reagents
• What is the function of the flame in atomic absorption
spectroscopy?
• A. Absorb the energy emitted from the metal analyte in returning
to ground state
• B. Supply the thermal energy needed to excite the metal analyte
• C. Bring the metal analyte to its ground state
• D. Supply the light that is absorbed by the metal analyte
• Most atomic absorption spectrophotometers
incorporate a beam chopper and a tuned
amplifier. The purpose of these components
is to avoid errors that would be caused by
• A. Variations in flame temperature
• B. Deterioration of the hollow-cathode lamp
• C. Stray light from the hollow-cathode lamp
• D. Measurement of light emitted by the
analyte
• In assaying an analyte with a single-beam
atomic absoiption spectrophotometer, what is
the instrument actually measuring?
• A. Intensity of light emitted by the analyte on
its return to the ground state
• B. Intensity of light that the analyte absorbs
from the hollow-cathode lamp
• C. Intensity of light that the analyte absorbs
from the flame
• D. Intensity of the beam from the hollow
cathode lamp after it has passed through the
analyte-containing flame
• Which of the following isolates
light within a narrow region of the
spectrum?
• A. Photomultiplier tube
• B. Monochromator
• C. Photovoltaic cell
• D. Detector
• Which of the following is the principle of
Flame emission photometers?
a) Radiation is absorbed by non-excited atoms
in vapour state and are excited to higher
states
Question b) Medium absorbs radiation and transmitted
radiation is measured
c) Colour and wavelength of the flame is
measured
d) Only wavelength of the flame is measured
29
2
• In Flame emission
photometers, the
measurement of _____________
is used for qualitative analysis.
a) Colour
b) Intensity
c) Velocity
d) Frequency
30
2
• In Flame emission
photometers, the
measurement of _____________
is used for quantitative
analysis.
a) Colour
b) Intensity
c) Velocity
d) Frequency
31
4
• Which of the following is not
an advantage of Laminar flow
burner used in Flame
photometry?
a) Noiseless
b) Stable flame for analysis
c) Efficient atomization of
sample
d) Sample containing two or
more solvents can be burned
efficiently
32
5
• In Flame photometry, Flame
color’s intensity tells about
A. how much of the element
present
B. Number of compound
present
C. complex structure of
compound
D. none of above
33
6
• Which of the following is the principle
of Flame emission photometers?
A. Radiation is absorbed by non-excited
atoms in vapour state and are excited to
higher states
B. Medium absorbs radiation and
transmitted radiation is measured
C. Colour and wavelength of the flame is
measured
D. Only wavelength of the flame is
measured
34
7
• which of the following is the
principle of Atomic Absorption
Spectroscopy?
a) Radiation is absorbed by non-
excited atoms in vapour state and
are excited to higher states
b) Medium absorbs radiation and
transmitted radiation is measured
c) Colour is measured
d) Colour is simply observed
35
• Which of the following is not a
component of the emission
system in Flame photometer?
a) Burner
b) Atomiser
c) Fuel gases and their
regulation
d) Chopper
36
• Which of the following statement is
false about double beam
absorption instruments?
a) It is similar to single beam
instruments except two beams are
present
b) Tungsten bulb is used as a source
c) Reference beam must have a
higher intensity than sample beam
d) Both the beams after they pass
through respective samples are
compared
37
10
• Beer’s law states that the
intensity of light decreases
with respect to ___________
a) Concentration
b) Distance
c) Composition
d) Volume
38
11
• Which of the following detectors is
used to detect light intensities
which are very weak?
a) Photomultiplier tube
b) Photovoltaic cell
c) Photoemissive tubes
d) Photo reflector
39

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Answers of the

• A. The most acidic sample is sample B.

• a) It must have a known output potential

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