Rick W. Staub, Ph.D.

Forensic Science and Related Consulting

7908 Ferndale Dr.
Plano, TX 75025
(972) 743-2228

REPORT OF RICK W. STAUB, PH.D.

I. ENGAGEMENT AND SCOPE OF WORK

1. I have been engaged by the law firm of Crowell & Moring, LLP for consultation

and testimonial service in the Anthony C. Apanovitch v. State of Ohio, Case No. CR84-194156.

II. SUMMARY OF EDUCATION AND PROFESSIONAL EXPERIENCE

2. I attach as Exhibit 1 my current Curriculum Vitae, which summarizes my

educational background, experience and expertise in the field of genetics and the forensic use of

genetic testing.

3. I received a B.S. in mathematics with honors from the University of Wisconsin

(Madison, WI) in 1974, a M.S. in genetics from the University of Arizona (Tucson, AZ) in 1978,

and a Ph.D. in genetics with a minor in molecular biology from the University of Arizona

(Tucson, AZ) in 1984. Since 2002, I also have been an Adjunct Clinical Assistant Professor at

the University of North Texas Health Science Center, Department of Pathology & Anatomy

(Fort Worth, TX).

 4. From 1984 to 1990, I worked as an Assistant Professor in the Biology Department

at Carleton College (Northfield, MN), where I taught genetics and molecular biology courses and

maintained an actively funded research program using molecular genetics techniques.

5. From 1990 to 1993, I worked as Associate Director and then Director at Genetic

Design (Greensboro, NC), where I was responsible for DNA operations in human identification.

6. From 1993 to 2000, I served as Director at Identigene, Inc. (Houston, TX), where

I developed the first STR-based human identification testing laboratory. This is generally the

same kind of testing done in this case that is the subject of my opinions expressed herein.

7. In 2000, I joined Orchid GeneScreen and Orchid Cellmark, of Dallas, Texas,

where I served as Director of Operations until 2004. From 2004 through 2012, I was the Senior

Manager and Laboratory Director at Orchid Cellmark in Dallas, Texas.

8. From 2012 through the present, I am the Chief Forensic Consultant for Staub

Forensic Genetics. In March 2013, I joined the Plano Police Department in Plano Texas, where I

serve as a CSI & Property/Evidence Unit Manager.

III. QUALIFICATIONS, COMPENSATION AND MATERIALS CONSIDERED

9. Since at least 1990 through the present, I have been involved in conducting DNA

testing, supervising others who conduct DNA testing, and interpreting the results of DNA

testing. I have testified as an expert in matters pending in many courts throughout the United

States as well as in some foreign countries. I hold a Certificate of Qualification as a Laboratory

Director in Forensic Identity and Parentage/Identity Testing from the New York State

Department of Health and have held this certification from 1996 to the present.

2
 10. In my capacity as a forensic DNA expert, I have acted as a consultant for a

number of states and municipalities, including the New York City Police Department, the Fort

Worth Police Department, the Houston Police Department, the Harris County Sheriff’s Office,

the Los Angeles Police Department, the Los Angeles County Sheriff’s Department, The Orange

County Sheriff’s Department, and the states of Illinois, Louisiana, Massachusetts, New Jersey,

Oklahoma, Washington, and Wisconsin. I have evaluated more than 1500 cases and have been

qualified and testified as an expert witness in more than 75 cases held across the United States.

11. Beyond my forensic experience, I have evaluated more than 100,000 cases in the

field of genetic testing to resolve genetic relationship issues, and have been qualified and

testified as an expert in more than 100 cases throughout the United States.

12. In addition to scientific and consultation services, I have authored, co-authored,

presented, or co-presented several dozen scientific papers and poster presentations addressing

issues in the fields of genetics and DNA testing. I have also presented scientific papers or poster

presentations in these areas at symposia and meetings of professional societies. I have authored

or co-authored papers that were published in the proceedings of various symposia and in peer-

reviewed publications, including in the Journal of Forensic Science, Transfusion, the Journal of

Heredity, and the American Journal of Human Genetics. I am also an inventor of three patents

on the development and use of STRs.

13. I attached as Exhibit 2 is a list of cases over the last four years in which I have

appeared.

14. I am compensated at a rate of $250/hour. My compensation is not related in any

way to the outcome of this litigation.

3
 15. The information I considered in forming the opinions, in addition to my own

knowledge and experience and any materials referenced herein, is provided as Exhibit 3.

IV. AN OVERVIEW OF FORENSIC DNA ANALYSIS

16. At the hearing, I plan to provide the Court with a tutorial on the relevant

background of the science of DNA, the use of DNA forensic testing and how that testing was

done by the Cuyahoga County Medical Examiner (“CCMEO”) in 2000 and 2001 on biological

samples representing samples taken from the vaginal and the oral cavities of the victim, Mary

Anne Flynn, during her autopsy on August 25, 1984. I intend to use material from basic texts in

the area and reference materials, including publications of the U.S. Department of Justice and the

National Institute of Justice, on DNA science and its application to forensic science, as well as

illustrations based on my experience and knowledge. I enclose as Exhibit 4 a list of some of the

texts or sources I may rely on for this tutorial.

17. This tutorial will include a contextual explanation of DNA, short tandem repeats

(STRs), loci and alleles and how they are used in DNA testing. I will describe commonly used

DNA test kits and techniques, including those used by CCMEO here. I will explain each step in

the process, including the extraction of DNA from cellular material and the differential

extraction designed to separate DNA from sperm cells (also known as male source or male

fraction) from DNA from a female source (also known as female fraction or epithelial fraction).

Then I will explain how the DNA recovered is quantified and them amplified by a process

known as the polymerase chain reaction (commonly known as “PCR”). I intend to explain how

the PCR technique uses repeated heating cycles to multiply (or amplify) the recovered DNA,

making literally billions of copies. In this process, differentially extracted DNA is amplified to

quantities sufficient in number to be separated, detected and identified by the next process, which

4
is capillary electrophoresis or electrophoresis. I will explain how electrophoresis is done and

how it was done here by CCMEO. I also will explain how the product of electrophoresis – the

electropherogram – is made and analyzed, and how the electropherograms made by CCMEO in

this case are analyzed.

18. I will also explain how forensic DNA testing is done to generate from unknown

samples major DNA profiles that then may be compared to known profiles to potentially include

or exclude a person as the source of DNA taken from a piece of evidence. I also will explain the

ultimate conclusions of inclusion or exclusion that can be made here and how those conclusions

were reached.

V. OVERVIEW OF THE CUYAHOGA COUNTY MEDICAL EXAMINER

OFFICE’S DNA TESTING

19. To maintain consistency and control the quality of testing, forensic DNA

laboratories develop standard procedures which are always followed when performing the

testing. These are described in a detailed manual that is typically called the Standard Operating

Procedures of the laboratory. Many of these procedures are based on process validations that

were performed in the laboratory prior to the initiation of any testing, and adherence to them is

essential to verify accuracy of results.

20. I have reviewed two copies of a detailed “STR Manual” dated July 2000 and

approved by Dr. Elizabeth Balraj, that were in effect during all periods when CCMEO conducted

the testing applicable to the Apanovitch case.1 These manuals include well-accepted and

necessary controls – both positive and negative – and procedures that have been validated to

1
CCMEO00000198-449.

5
ensure the production of reliable data from evidence containing DNA, and the reliable

interpretation of those data. I will explain the role of these procedures and controls in my

interpretation of, and reliance on, the data generated by CCMEO.

21. From 2000-2001, CCMEO tested a total six items, or slides, representing samples

taken from the victim, Mary Anne Flynn during autopsy.2 Based on my review of the laboratory

examination report (“CCMEO Report”) and supporting documents, these items all originated

from swabs taken by Dr. Balraj during the autopsy of Mary Anne Flynn.3 Swabs were taken

from the oral, vaginal and rectal cavities. Spermatozoa were identified in the samples taken from

the oral and vaginal cavities, but no spermatozoa were identified in the rectal samples.4 From the

available record, the CCMEO performed DNA testing only on samples taken from the victim’s

oral and vaginal cavities.5 The swabs generated by Dr. Balraj during the autopsy were used to

create two sets of slides: Trace Evidence slides and Pathology slides.

22. The specific slide numbers for the Trace Evidence that are discussed below are as

follows:

i. Items 1.1 (Swab of vaginal smear slide)

ii. Item 1.2 (Scrapings of vaginal smear slide)

iii. Item 2.1 (Swab of oral smear slide)

2
CCMEO00000645, 703.
3
Id.
4
Id.
5
CCMEO00000128-131.

6
 23. I understand from the CCMEO file that the Pathology slides were retrieved in

2001 at the request of one of the technicians for testing after the Trace Evidence slides were

tested.6 The Pathology slide numbers, along with a description and date of testing, are as

follows:

i. Item 3.1 (Scrapings of vaginal smear slide – tested May 2001)

ii. Item 5 (Swab of oral smear slide – tested September 2001)

iii. Item 6.1 (Scrapings of oral smear slide – tested May 2001)

24. I attach a chart summarizing the results from the CCMEO testing (“Allele

Summary Chart”) as Exhibit 5. The Allele Summary Chart that I have prepared matches almost

entirely with the chart and conclusions reached by the CCMEO, with one exception that I will

discuss below.7

VI. ITEMS TESTED THAT GENERATED NO USEFUL DNA DATA

25. The majority of the testing efforts made by CCMEO yielded no data from which

relevant conclusions could be drawn. Items 1.1, 2.1, 3.1, 5, and 6.1 fall into this category. Each

of these items failed to generate electrophoresis peaks of sufficient amplitude to be called peaks

and to be relied upon for analysis. Specifically, the data from these items failed to result in any

peaks of at least 200 RFU in amplitude, which was required by the Standard Operating

6
CCMEO00000132.
7
CCMEO00000052 (Locus D8S1179).

7
Procedures and methods employed by the CCMEO before an allele peak could be called or

identified.8

26. Item 1.1 is taken from a swabbing of a trace evidence slide representing material

taken from the victim’s vaginal cavity during autopsy.9 According to CCMEO records that I

have reviewed, this slide was opened and swabbed on November 8, 2000 and a differential

extraction was performed on that day for the purpose of obtaining a sperm or male DNA fraction

and an epithelial fraction for amplification and testing.10 The electrophoresis done on the

epithelial fraction yielded some potential below-threshold results at six STR loci and the AMEL

(sex identifying) locus, that if identified as peaks would be consistent with Mary Anne Flynn.

The CCMEO Report notes that epithelial fraction yielded a “partial profile,” which is consistent

with my analysis. I note, however, that the signal at each of the potential peaks is lower than the

unit threshold of 200 RFU, which is the validated threshold required by the CCMEO SOP to

“call” a peak.11 The electrophoresis completed on the male fraction, however, yielded no results,

which is consistent with CCMEO’s conclusion that “no human DNA could be obtained.” 12 In

my professional opinion, Item 1.1 does not contain any useful data relevant to the Apanovitch

matter.

27. Item 2.1 is a scraping of a trace evidence slide smear taken from the vaginal

cavity during autopsy.13 CCMEO performed a differential extraction on Item 2.1, but the results,

after amplification, yielded an electrophoresis from which few peaks can be called for either

8
CCMEO00000299-300.
9
CCMEO00000127.
10
CCMEO00000106, 108, 115, 117, 120.
11
CCMEO00000052 (Locus D8S1179).
12
CCMEO0000047.
13
CCMEO0000048.

8
fraction. For the epithelial fraction, eight STR loci produced allelic peaks, which resulted in a

partial profile.14 Although most of these peaks are below the allele calling threshold, that partial

profile excludes Ms. Flynn.15 The electropherogram for the male fraction yielded no results. My

analysis concurs with the CCMEO Report, which stated that the epithelial fraction was a “partial

profile and a mixture.”16 In my professional opinion, Item 2.1 does not contain any useful data

relevant to the Apanovitch matter.

28. Item 3.1 is a scraping of a pathology slide made from a vaginal smear slide taken

during autopsy.17 CCMEO performed a differential extraction on Item 3.1 on or about May 10,

2001 and generated the electropherogram, after amplification, on or about May 16, 2001.18 The

electrophoresis for the epithelial fraction was characterized by very poor amplification with

possible below-threshold signal for D5 and AMEL.19 My conclusion is consistent with the

CCMEO Report, which stated that there was “insufficient human DNA” that could be “isolated”

from Item 3.1.20 The electrophoresis for the male fraction generated no results, which is also

consistent with the findings in the CCMEO Report.21 In my professional opinion, Item 3.1 does

not contain any useful data relevant to the Apanovitch matter.

29. Item 5 is an oral smear pathology slide.22 It was made by swabbing the slide,

drying the swabs, cutting the swab tips off and using them for the extraction.23 CCMEO

14
CCMEO0000056.
15
See Exhibit 5 (chart).
16
CCMEO0000047.
17
CCMEO0000048.
18
CCMEO0000119, 123, 116, 113.
19
CCMEO00000073.
20
Id.
21
CCMEO0000047, 76.
22
CCMEO00000122.
23
Id.

9
performed a differential extraction on Item 5 on September 19, 2001.24 The efforts to amplify

the DNA in this sample were apparently not successful as the CCMEO stopped processing the

slide after DNA quantification indicated no DNA present and no electrophoresis was

performed.25 My observations concerning Item 5 are consistent with the CCMEO Report, which

stated in both the epithelial and male fractions, “no human DNA could be obtained.”26 In my

professional opinion, Item 5 does not contain any useful data relevant to the Apanovitch matter.

30. Item 6.1 is a scraping of a pathology slide made from an oral smear taken during

autopsy.27 CCMEO performed a differential extraction on or about May 10, 2001.28 The

electrophoresis of the epithelial fraction was characterized by poor amplification and signals well

below the 200 RFU threshold amplitude required by CCMEO’s validated SOP for calling

alleles.29 Any results in the electropherogram would not comply with the SOP. Although there

are no notes, the CCMEO technician apparently lowered the threshold in Applied Biosystems’

Genotyper software from 200 RFU to 50 RFU in an effort to ascertain whether there were peaks

at this low level signal. Even at this level, the software failed to identify peaks, or call alleles,

for eight of nine STR loci (excluding the AMEL sex determining loci) tested on the epithelial

fraction.30 Similarly at this low level, the software failed to identify peaks, or call alleles, for six

of the nine STR loci (excluding the AMEL sex determining loci) tested on the male fraction.31

The CCMEO Report concurs with my conclusions, stating that “insufficient human DNA was

24
CCMEO00000118.
25
CCMEO00000107.
26
CCMEO00000047.
27
CCMEO00000123.
28
CCMEO00000119.
29
CCMEO00000299-300.
30
CCMEO0000077.
31
OHP0000098/4753.

10
isolated” and that “no profiles could be obtained.”32 In my professional opinion, Item 6.1 does

not contain any useful data relevant to the Apanovitch matter.

VII. TESTING ON ITEM 1.2 EXCLUDES MR. APANOVITCH AS THE SOURCE OF

MALE DNA FROM THE VAGINAL SWAB AND PRODUCED A MAJOR MALE
PROFILE THAT MAY BE COMPARED TO KNOWN MALE DNA PROFILES

31. Among the Items tested by CCMEO, only one yielded results from which relevant

conclusions could be drawn.

32. Item 1.2 is a scraping of a trace evidence slide made from a vaginal smear taken

during autopsy.33 Like the other Items, analysis of Item 1.2 shows that sperm was present on the

slide.34 CCMEO performed a differential extraction. The electropherograms for Item 1.2

produced peaks substantially above threshold in both the male fraction and the epithelial

fraction.35 Accordingly, these data are useful and can be analyzed further to determine a DNA

profile for the male fraction that can be compared to Mr. Apanovitch and to other known

profiles.

33. The electropherogram for the epithelial fraction for Item 1.2 yielded alleles at five

of nine loci tested plus the AMEL (sex identifying locus), all of which are consistent with the

victim Mary Anne Flynn.36 The amplitude of each of these peaks is well above the threshold 200

RFU.37 These data are useful as a control to confirm that Item 1.2 that was subjected to DNA

testing in 2000 by CCMEO was taken from a swab of the victim’s vaginal cavity created at

autopsy.

32
CCMEO00000047.
33
CCMEO00000047, 127.
34
CCMEO00000046, 127.
35
CCMEO00000108.
36
CCMEO00000094.
37
Id.

11
 34. The electropherogram for the male fraction for Item 1.2, yielded alleles at all nine

loci tested plus the AMEL (sex identifying loci). These data contain more than two alleles at a

number of loci and thus represent a mixture of DNA, that is, this sample contains the DNA from

one contributor, at least one of whom is male. CCMEO also concluded that this male fraction of

Item 1.2 contained a mixture.38

35. It is well accepted that from mixtures of this kind, a major male profile may be

determined by identifying the major allele peaks present in the sample to identify a single source

that represents the major portion of the male DNA present. My Allele Summary Chart reflects

this process of assigning alleles to major and minor sources. In this chart, alleles below the

CCMEO allele calling threshold are designated with an asterisk. These asterisked alleles would

be the same as alleles in parentheses in the CCMEO data. An excerpt from my Allele Summary

Chart below shows the major and minor profiles below:

Sample 1.2 MF Deduced 1.2 MF Likely minor profile

major male assuming two contributors
D3S1358 16,17 15*,18*
vWA 16,18 14*,15*
FGA 23,25 22*,?
AMEL X,Y X or X,Y
D8S1179 10,13 13,14
D21S11 30,32.2 2 of 31.2*, 27*,28*,29*
D18S51 15 13*,14*
D5S818 12 2 of 10*,11*,13*
D13S317 8 8,11* or 11*
D7S820 10,12 8*; 8*,10; or 8*,12

38
CCMEO00000047.

12
 36. This assessment of the profiles matches a chart created by the CCMEO, except

that at loci D8S1179, I have assigned 14 to the minor profile in light of the fact that its peak is

smaller than 10, 13.39

37. The DNA from Item 1.2 was extracted into a male and epithelial fraction and the

male fraction represents a complete major male profile that excludes Mr. Apanovitch.40

Moreover, Mr. Apanovitch is excluded as the source of a minor profile in Item 1.2 based on the

absence of alleles consistent with the alleles of Mr. Apanovitch at multiple loci, including at

D3S1358, FGA, D21S11, D18S51, D13S317, and D7S820.

38. No CCMEO testing of the Items derived from slides made from swabs taken in

the autopsy identify Mr. Apanovitch’s DNA profile. Item 1.2 is also the only sample tested that

generated DNA data above the threshold or level required to identify, or call, an allele, and it

unquestionably excludes Mr. Apanovitch.

39. The major male DNA profile that I have developed from CCMEO’s testing of

Item 1.2 could have been generated by CCMEO in 2000/2001 and it appears that they did

identify a major profile within that timeframe and investigated the possibility of comparing that

profile to ones located in BCII databases. It is my opinion that a comparison could be made

today.

VIII. SUMMARY OF CONCLUSIONS

40. The CCMEO conducted DNA testing on six items representing samples taken

from oral and vaginal swabs of the victim during her autopsy on August 25, 1984. DNA testing

39
CCMEO00000052.
40
CCMEO00000053; BCII00000105, 117.

13
was done on six of these Items in the 2000-2001 time period by the Cuyahoga County Medical

Examiner’s Office. For five of these Items, there was insufficient data from which to draw any

conclusions. These data, among other problems, represented electropherogram signal well below

the standard threshold level of 200 RFU required in the CCMEO methods to call or identify an

allele.

41. The DNA testing conducted by CCMEO on Item 1.2 – a sample originating from

a vaginal swab taken at autopsy – generated signal well above the standard threshold for calling

alleles. Item 1.2 was subjected to differential extraction to extract DNA and separate DNA in the

sperm (male fraction) from DNA in the non-sperm (epithelial or female fraction) DNA. For the

epithelial fraction, the partial allele profile is above threshold and consistent with it coming from

a female and that female being the victim. This provides a useful control that this sample is what

it purports to be: a sample of biological material taken from Ms. Flynn’s vagina at autopsy. For

the male fraction, the alleles present exclude Mr. Apanovitch as the source of the male DNA

extracted from swabs taken from the victim’s vaginal cavity at autopsy.

42. The male DNA present in this sample is from more than one source, but there is a

major source that represents the major portion of the male DNA present. That major source

represents a complete major profile that, once again, excludes Mr. Apanovitch and which

moreover could be compared to other known profiles available to the state. The CCMEO has

had these data and major profile since the 2000-2001 period of time.

Dated: September 15, 2014 ____________________________________

Rick W. Staub, Ph.D.

14
EXHIBIT 1
 Curriculum Vitae

Rick W. Staub, Ph.D.

7908 Ferndale Drive 972.208.5484 r.
Plano, TX 75025 [email protected] 972.743.2228 c.

Education

Ph.D. Genetics (Minor in Molecular Biology), University of Arizona, Tucson, AZ 1984

M.S. Genetics, University of Arizona, Tucson, AZ 1978

B.S. Mathematics with Honors, University of Wisconsin, Madison, WI 1974

Professional Experience

CSI & Property/Evidence Unit Manager, Plano Police Department, Plano, TX, 3/2013 –
Present.

Chief Forensic Consultant, Staub Forensic Genetics, 2012 – Present.

Forensics Laboratory Director, Scientific Operations, Orchid Cellmark, Dallas, TX, 2008
to 2012. Technical Leader, Y-STR & mtDNA, 1/2009 – 2012. Technical Leader,
Autosomal STR, 5/2009 – 5/2010. R&D Director, 2008 – 2011.

Senior Manager and Laboratory Director, Orchid Cellmark, Dallas, TX, 2004 to 2008.
Acting Technical Leader, 9/2007 to 7/2008.

Adjunct Clinical Assistant Professor, University of North Texas Health Science Center,
Department of Pathology and Anatomy, Fort Worth, TX, 2002 to 2008, 2012 - Present.

Director of Operations and Laboratory Director, Orchid GeneScreen and Orchid

Cellmark, Dallas, TX, 2000 to 2004.

Laboratory Director, Identigene Inc., Houston, TX, 1994 to 2000.

Laboratory Director, Laboratory for Genetic Services, Houston, TX, 1993 to1998.

Director of DNA Operations, Genetic Design, Greensboro, NC, 1992 to1993.

Associate Director, Genetic Design, Greensboro, NC, 1990 to 1992.

Curriculum Vitae
Rick W. Staub, Ph. D
Page 1 of 16
Rev 7/2013
Assistant Professor of Biology, Carleton College, Northfield, MN 1984 to1990.

Biology Instructor, Carleton College, Northfield, MN, 1982 to 1984.

Research Cytogeneticist, University of Arizona, Plant Sciences Department, Tucson, AZ,

1978 to 1982.

Level of Training Completed

Doctoral Laboratory Director since 1992.

Certifications

Laboratory Director by the New York State Department of Health, 10/3/96 to present

Professional Associations

American Society of Crime Laboratory Directors, Member

International Society for Forensic Genetics, Member

American Academy of Forensic Sciences, Member

Association of Forensic DNA Analysts and Administrators, Member

AABB (formerly American Association of Blood Banks), Member

Genetics Society of America (Past member)

American Genetics Association (Past member)

American Association for the Advancement of Science (Past member)

Sigma Xi Scientific Research Society (Past member)

National Child Support Enforcement Association Corporate Speakers' Bureau (Past

member)

Parentage Testing

Cases evaluated: more than 100,000

Curriculum Vitae
Rick W. Staub, Ph. D
Page 2 of 16
Rev 7/2013
Trials: Testified as an expert in the field of disputed paternity in more than 100 cases
tried throughout the United States.

Forensic Testing

Cases evaluated: more than 1500

Trials: Testified as an expert in the field in more than 75 cases held across the United
States and abroad

Honors

B.S. in Mathematics with Honors, University of Wisconsin, Madison, WI 1974

Sigma Xi Scientific Research Society

Patents

United States Patent 6,458,537. Staub, Rick W. and Carrico, Michael G. Methods of
DNA typing with tandem repeats. October 1, 2002.

United States Patent 6,187,540. Staub, Rick W. and Caskey, Caroline. Method of
newborn identification and tracking. February 13, 2001.

United States Patent 5,994,064. Staub, Rick W. and Carrico, Michael G. Simple and
complex tandem repeats with DNA typing method. November 30, 1999.

Publications

Staub, R.W. and Johnson, C.L. 2009. Determining Non-Excluded Haplotypes in the
Yfiler® Database for Y-STR Mixtures. Applied Biosystems Forensic News. April,
2009.
http://marketing.appliedbiosystems.com/images/All_Newsletters/Forensic_0409/articles.
html

Johnson, C.L. and Staub, R.W. 2009. 10 Tips for Testifying to Y-STR Results. Applied
Biosystems Forensic News. April, 2009.
http://marketing.appliedbiosystems.com/images/All_Newsletters/Forensic_0409/articles.
html

Curriculum Vitae
Rick W. Staub, Ph. D
Page 3 of 16
Rev 7/2013
Staub, R.W. and Johnson, C.L. 2008. Analyzing Y-STR mixtures and calculating
inclusion statistics. Proceedings of the 19th International Symposium on Human
Identification:
http://www.promega.com/geneticidproc/ussymp19proc/oralpresentations/Staub.pdf

Okagaki, R.J., Jacobs, M.S., Stec, A.O., Kynast, R.G., Buescher, E., Rines, H.W., Vales,
M.I., Riera-Lizarazu, O., Schneerman, M., Doyle, G., Friedman, K.L., Staub, R.W.,
Weber, D.F., Kamps, T.L., Amarillo, I.F.E., Chase, C.D., Bass, H.W., and Phillips, R.L.
2008. Maize centromere mapping: A comparison of physical and genetic strategies.
Journal of Heredity 2008 99(2):85-93.

Scarpetta, M.A., Staub, R.W., and Einum, D.D. 2007. Assessing the exclusionary power
of a paternity test involving a pair of alleged grandparents. Transfusion 47: 335-340.

Johnson C.L., Giles R.C., Warren J.H., Floyd, J.I. and Staub, R.W. 2005. Analysis of
Non-Suspect Samples Lacking Visually Identifiable Sperm Using a Y-STR 10-Plex.
Journal of Forensic Science 50(5): 1116-1118.

Johnson C.L., Warren J.H., Giles R.C., Staub R.W. 2003. Validation and Uses of a Y-
chromosome STR 10-plex for forensic and paternity laboratories. Journal of Forensic
Science 48:1260-1268.

White, L.D., Shumaker, J.M., Tollett, J.J., and Staub, R.W. 1998. Human Identification
by Genotyping Single Nucleotide Polymorphisms (SNPs) Using an APEX Microarray.
Proceedings from the Ninth International Symposium on Human Identification. Promega
Corporation. http://www.promega.com/geneticidproc/ussymp9proc/content/07.pdf

Staub, R.W. and R.A. Bever. 1992. A comparison of regional VNTR RFLP databases.
Proceedings from the Third International Symposium on Human Identification. Promega
Corporation. p.191-198.

Bever, R.A., R.W. Staub, and B.W. Meehan. 1991. Characterization of five VNTR loci
by Hae III RFLP analysis: Application to paternity testing. Proceedings from the
Second International Symposium on Human Identification. Promega Corporation. p.
103-128.

Staub, R.W. 1987. Leaf striping correlated with the presence of B chromosomes in maize.
Journal of Heredity 78:71-74.

Staub, R.W. 1984. The influence of B chromosomes on the susceptibility of maize to

gamma irradiation induced DNA damage. Doctoral Dissertation, University of Arizona.

Staub, R.W. 1982. The effect of B chromosomes on the susceptibility of maize to gamma
radiation. New Mexico Journal of Science 22:16.

Curriculum Vitae
Rick W. Staub, Ph. D
Page 4 of 16
Rev 7/2013
Abstracts, Presentations, Posters and Notes

Cotton, Robin W. & Staub, Rick W. Challenges of Low-Level DNA Samples. Webinar
presented for Innocence Project of New York. April 2, 2013.

Staub, Rick W., Dmitry Mashchenski, Carlos Baena, Pedro Reinoso, and Sid Sinha. A
Study of the Effects of Database Size and Loci Number on the Potential for Successful
Familial Searches in Databases of STR Profiles. Oral presentation at the 64th Annual
Scientific Meeting of American Academy of Forensic Sciences. Atlanta, GA. February
24, 2012.

Staub, Rick W. Solving Crime in the 21st Century: How DNA can help you win cases.
Presentation to prosecutors, investigators and other law enforcement agency officers.
McLennan County District Attorney’s Office. Waco, TX. November 10, 2011.

Staub, R.W., J. Boschwitz, S.K. Sinha, C. Ramos, P. Reinoso, S. Dineen, C. Holovach, C.

Baena, and D. Maschenski. A software application to manage and search DNA profiles
and perform familial searches. Poster presentation at 22nd International Symposium on
Human Identification. National Harbor, MD. October 3-6, 2011.

Ayers, S., R.W. Staub, S.K. Sinha, and A.K. LeFebvre. A Comparison of Concentration
Methods for Low Copy Number Forensic DNA Analysis. Poster presentation at 22nd
International Symposium on Human Identification. National Harbor, MD. October 3-6,
2011.

Smitherman, C.B., H. Nasir, W.L. Hoffman, R.W. Staub, and S.K. Sinha, Orchid
Cellmark’s Osteo-Pure™ Bone Extraction Procedure Captures Degraded DNA to
Improve STR Results. Poster presentation at 21st International Symposium on Human
Identification. San Antonio, TX. October 11-14, 2010.

Staub, Rick W. Get Wise to Ys: Understanding How Y-STRs Can Solve Criminal Cases.
Presentation at the Y-STR Analysis Workshop at the 2010 Annual Meeting of the
Northwest Association of Forensic Scientists. September 29, 2010.

Staub, Rick W. Relationship Testing Workshop at Division of Forensic Services,

Forensic Science Center at Chicago. September 21-22, 2010.

Staub, Rick W. Considerations for the Analysis of Low Level Forensic Samples.
Continuing Education Seminar presented at Orchid Cellmark. Dallas, TX. September
17, 2009.

Staub, Rick W. Latest Developments in DNA: It Ain’t Just Blood and Semen. Invited
speaker at Louisiana Association of Criminal Defense Lawyers CLE Seminar. December
5, 2008.

Curriculum Vitae
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Staub, Rick. Analyzing Y-STR Mixtures and Calculating Inclusion Statistics. Invited
speaker at the 19th International Symposium on Human Identification. Hollywood, CA.
October 13-16, 2008.

Analyzing Y-STR mixture profiles. Invited speaker at Summer Meeting of Association

of DNA Analysts and Administrators. Austin, TX. August 7, 2008.

Johnson, C.L., Floyd, J.I., and Staub, R.W. 2007. Over 300 Y-STR cases – What have
we learned? Proceedings of the American Academy of Forensic Sciences.

Einum, D.D., Staub, R. and Scarpetta, M. Assessing Exclusionary Power of a

Paternity Test Involving a Pair of Alleged Grandparents (Poster
presentation). 56th Annual Meeting of the American Society of Human
Genetics, New Orleans, Louisiana, October 9-13, 2006.

Staub, Rick. Products of Anonymous Sperm Donations. Invited speaker at the 2nd
Annual Present and Future Technological Advances in Human Identification Conference
sponsored by The Virginia Institute of Forensic Science and Medicine. Roanoke, VA.
March 27 - 29, 2006.

Johnson CL, Giles RC, Staub RW, Franco RI, Anguiano E, Smitherman CB,
Wickenheiser RA (2006). The Analysis of Latent Fingerprints Using Y-STR,
Mitochondrial, and SNP Testing. Proceedings of the American Academy of Forensic
Sciences.

Staub, Rick. Products of “Anonymous” Sperm Donations. Invited speaker at the 16th
International Symposium on Human Identification. Grapevine, TX. September 25-29,
2005.

Johnson C.L. and Staub R.W. 2005. A comparison of Y-STR multiplexes. Proceedings
of the American Academy of Forensic Sciences. 11:80-81.

Johnson C.L., Warren J.H., Floyd J.I., Staub R.W. 2004. Casework experience using Y-
STRs. Proceedings of the 15th International Symposium on Human Identification.

Staub, R.W. PI calculations in cases involving mixtures of maternal and fetal tissue.
Presentation at AABB Parentage Testing I: Scientific SIG. Baltimore, MD. October 23,
2004.

Johnson C.L., Giles R.C., Staub R.W. 2004. Examination of non-suspect samples
lacking sperm using a Y-STR 10-plex. Proceedings of the American Academy of
Forensic Sciences.

Johnson C.L., Warren J.H., Giles R.C., Staub R.W. 2003. Using Y-chromosome
multiplexes in forensic and paternity laboratories. Proceedings of the 14th International
Symposium on Human Identification.
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Anguiano, E., C. Johnson, R. Giles, R. Staub, and J. Baisch. 2003. The use of single
nucleotide polymorphism (SNP) markers from the NRY region of the human Y
chromosome for genealogical and forensic applications. Poster presented at the 14th
International Symposium on Human Identification. Phoenix, AZ. September 30 –
October 2, 2003.

Brenner, C.H. and R.W. Staub. 2003. Can DNA solve this? Poster presented at the 14th
International Symposium on Human Identification. Phoenix, AZ. September 30 –
October 2, 2003.

Johnson, C.L., J.H. Warren, R.C. Giles, and R.W. Staub. 2003. Using Y-chromosome
multiplexes in forensic and paternity laboratories. Poster presented at the 14th
International Symposium on Human Identification. Phoenix, AZ. September 30 –
October 2, 2003.

Staub, R.W. 2003. Optimization of a Y-STR multiplex for use on forensic specimens.
Invited speaker at Tennessee Bureau of Investigation’s “Forensic Applications of Y-
chromosome DNA Typing Symposium.” May 12, 2003 in Nashville, TN.

Johnson, C.L., A. Eisenberg, J. Planz, J.E. Warren, J.H. Warren, and R.W. Staub. 2002.
Validation of a Y-chromosome STR 10-plex. Poster presented at the 13th International
Symposium on Human Identification. Phoenix, AZ. October 7-10, 2002.

Staub, R.W., 2001. Implementation of a 96 capillary electrophoresis instrument with

PowerPlex 16 in a high-volume genotyping laboratory. Paper presented at the Twelfth
International Symposium on Human Identification. Biloxi, MS. October 11, 2001.

Staub, R.W., 2000. Luminex technology for paternity testing. Paper presented at the
Parentage Testing Forum of the AABB National Conference. Washington, D.C.,
November 4, 2000.

White, L.D., Tollett, J.J., Liles, L.A., Teske, J.P., Warren, J.H., Staub, R.W. The APEX
SNP identity chip and the IDENTIPLEX™ STR identity testing system. Presented at the
1999 Annual Meeting of the American Society of Human Genetics.
http://genetics.faseb.org/genetics/ashg99/f2334.htm

Staub, R.W., 1999. STRs v. SNPs for paternity testing. Paper presented at the Tenth
International Symposium on Human Identification. Orlando, FL. September 29 -
October 2, 1999.

Staub, R.W., 1999. Single nucleotide polymorphism (SNP) analysis for human
identification using APEX microarrays. Invited speaker at Cambridge Healthtech
Institute’s Third Annual Symposium on DNA Forensics. June 13-15, 1999. McLean,
VA.

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White, L.D., J.M. Shumaker, J.J. Tollett, and R.W. Staub. 1998. A microarray chip-
based method for scoring single nucleotide polymorphisms (SNPs). American Journal of
Human Genetics. 63:A244. Paper presented at Annual Meeting of American Society of
Human Genetics. Denver, CO. October 27 – 31.

Warren, J.H., M.G. Carrico, and R.W. Staub. 1998. Real time paternity testing utilizing
a novel 11 system STR multiplex analyzed on an ABI Prism 377 DNA Sequencer. Poster
presented at the Ninth International Symposium on Human Identification. Orlando, FL.
October 8-10, 1998.

White, L.D., J.M. Shumaker, J.J. Tollett, and R.W. Staub. 1998. Human identification
by genotyping single nucleotide polymorphisms (SNPs) using an APEX microarray.
Paper presented at the Ninth International Symposium on Human Identification.
Orlando, FL. October 8-10, 1998.

Staub, R.W. 1997. The utilization of complex and simple tandem repeats in human
identity typing: Experiences from a commercial laboratory. Invited speaker at
Cambridge Symposium on DNA Forensics: Science, Practice and Future Prospects.
November 17-18, 1997. McLean, VA.

Carrico, M.G. and R.W. Staub. 1997. Death, DNA and Dollars: The story of one man's
posthumous struggle with his infidelity. Poster presented at the Eighth International
Symposium on Human Identification. Scottsdale, AZ. September 17-21, 1997.

Smith, J.L., S.W. Cheung, R.W. Staub, and A. Faucett. 1996. Identification of
mosaicism for a small Y marker chromosome cell line by F.I.S.H. American Journal of
Human Genetics. 59:s1921. Poster presented at the Annual Meeting of the American
Society of Human Genetics. San Francisco, CA. October 29 – November 2, 1996.

Shumaker, J.M., J.J. Tollett, K.J. Filbin, B.B. Roa, C.S. Richards, S.H. Nue, R. Staub,
and M.Pirrung. 1996. Rapid detection of BRCA1, p53, and CFTR mutations by arrayed
primer extension. American Journal of Human Genetics 59:s1647. Poster presented at
the Annual Meeting of the American Society of Human Genetics. San Francisco, CA.
October 29 – November 2, 1996.

Staub, R.W., M.G. Carrico, J.J. Tollett, S. Nye, M. Pirrung, and J.M. Shumaker. 1996.
Arrayed primer extension analysis of single nucleotide polymorphisms (SNPs) for human
identification. Paper presented at the Seventh International Symposium on Human
Identification. Scottsdale, AZ. September 19-21, 1996.

Staub, R.W. 1996. Paternity Testing: Key Issues for Family Law Practitioners. Paper
presented at the 14th Annual Family Law Institute. Sponsored by the Family Law
Section, State Bar of Georgia, Institute of Continuing Legal Education in Georgia. May
23-25, 1996.

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Staub, R.W. 1996. Paternity Testing. Presentation at the Third Annual Hot Tips Family
Law Seminar. Sponsored by the Family Law Section of the Mississippi Bar. Reservoir
Lodge, Ridgeland, MS. May 17, 1996.

Staub, R.W. 1996. Powerful new STR multiplexes for use in human identification.
Invited speaker at Cambridge Symposium on DNA Forensics: Science, Practice and
Future. April 21-27, 1996. Santa Fe, NM.

Staub, R.W. 1996. Who's the Father? Current Trends in Paternity Testing. Seminar
presented to the Tennessee Bar Association Conference on 1996 Family Law Issues.
Memphis, TN. March 8, 1996. Nashville, TN. March 22, 1996.

Endean, D.J., and Staub, R.W. 1995. Update on Mutations at VNTR Loci. Parentage
Testing Forum. American Association of Blood Banks Annual Meeting. New Orleans,
LA. November 14, 1995.

Staub, R.W. and M. Carrico. 1995. Molecular analysis of a mutational event at the
HUMHPRTB locus detected by a single child / father non-match at the (AGAT)n STR
region. Poster presented at the Sixth International Symposium on Human Identification.
Scottsdale, AZ. October 12-14, 1995.

Matson, R.S., P.J. Coassin, J.B. Rampal, and R.W. Staub. 1995. Probe-target
interactions on disposable plastic film based array panels. Poster presented at The
Genetic Screening & Diagnosis of Human Disease Conference, Cambridge Healthtech
Institute. San Francisco, CA. March 8-10, 1995.

Staub, R.W. and M. Carrico. 1994. Performing a Paternity Analysis with a Battery of
STR Systems Utilizing DNA Obtained from Archived Cytogenetic Slides of Fixed
Human Bone Marrow Cells. Poster presented at the Fifth International Symposium on
Human Identification. Scottsdale, AZ. October 8-11, 1994.

Staub, R.W. 1993. DNA Typing in Human Identification. Seminar presented to the St.
Olaf College Biology Department, Northfield, MN. May 3, 1993.

Staub, R.W., M. DeGuglielmo, and R. Bever. 1993. Comparisons Among Regional

VNTR RFLP Racial Databases. Poster presented at The Second International
Symposium on Forensic DNA Analysis. Federal Bureau of Investigation Academy.
Quantico, VA. March 29 - April 2, 1993.

Staub, R.W. 1993. General Overview of HLA and DNA Paternity Blood Testing.
Paternity Forum presented at the LCSEA Conference, Lafayette, LA. March 3, 1993.

Staub, R.W. 1993. Genetic Identification of Humans. Seminar presented to the High
Point University American Chemical Society Affiliates Club at High Point University,
High Point, NC. February 2, 1993.

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Staub, R.W. 1993. Interpretation of Results and Understanding Calculations. Sponsored
by the State of Louisiana Department of Social Services, Paternity Blood Testing Issues.
Baton Rouge, LA. January 14, 1993.

Staub, R.W. 1992. Interpretation of Results. Seminar presented at the National Child
Support Enforcement Association Workshop, Paternity Establishment: Issues for the
90's. Atlantic City, NJ. December 11, 1992.

Staub, R.W. 1992. Understanding HLA Blood Test Results. Conference sponsored by
the Arizona Family Support Council. Tucson, Arizona. November 20, 1992.

Staub, R.W. 1992. 41st Annual Training Conference and Exposition, Establishment of
Parentage, To Be or Not To Be: Is That the Question? National Child Support
Enforcement Association. Annual Conference. Orlando, FL. August 25, 1992.

Staub, R.W. 1992. Interpretation of Results. Seminar presented at the National Child
Support Enforcement Association Workshop, Paternity Establishment: Issues for the
90's. Honolulu, HI. July 7, 1992.

Staub, R.W. 1992. How to Read/Interpret HLA/DNA Results. Seminar presented to the
District Attorney Appalachian Judicial Circuit. Child Support Recovery Unit. May 14,
1992.

Staub, R.W. 1992. North Dakota Family Support Council. Third Annual Conference.
Bismarck, ND. March 30 - April 1, 1992.

Staub, R.W. 1992. Agricultural Biotechnology: Plant Transformation Techniques.

Seminar presented to the Biology Department at the University of North Carolina at
Greensboro. March 5, 1992.

Staub, R.W. 1992. Polymerase chain reaction in DNA technology. Seminar presented at
High Point University, High Point, NC. February 4, 1992.

Staub, R.W. 1992. DNA technology as related to the genetic identification of humans.
Seminar presented at High Point University, High Point, NC. February 4, 1992.

Staub, R.W. 1992. Interpretation of Results. Mississippi Child Support Conference.

January 15 - 16, 1992.

Staub, R.W. 1991. Discussion on Various Blood Testing Techniques, How Samples are
Analyzed and Interpretation of Results. Sponsored by the Office of Child Support
Enforcement, Syracuse, NY. December 17, 1992.

Staub, R.W. 1991. 10th Annual New Jersey Child Support Conference, The 2nd Decade:
Continuing the Commitment. Sponsored by The New Jersey Child Support Council,
Atlantic City, NJ. December 11 - 12, 1991.
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Staub, R.W. 1991. Mock Trial. Paternity Establishment: Issues for the 90's. Sponsored
by the North Carolina Support Enforcement Agency. Washington, DC. November 13 -
16, 1991.

Creacy, S.D., B.W. Meehan, R.W. Staub, and R.A. Bever. Allele frequency distribution
and characterization of five single-locus probes within and among populations in North
America. American Journal of Human Genetics s49 (4): 500 (#2854). Poster presented
at the 8th International Congress of Human Genetics. October 6 -11, 1991.

Staub, R.W. 1991. Invited speaker at 122nd Annual Summer Conference. Sponsored by
the New York Public Welfare Association. Catskill, NY. July 14 - 17, 1991.

DeGuglielmo, M., R.W. Staub, and R.A. Bever. Population Analysis of two recently
characterized polymorphic VNTR loci. Poster presented at the Annual Meeting of the
Canadian Society of Forensic Sciences. October 2, 1990.

Rose, K.L. and R.W. Staub. Centromeric breakage and the recovery of both telocentric
fragments. Maize Genet. Coop. News Lett. 64:94-95.

Rose, K.L. and R.W. Staub. Isolation and cytological analysis of two telocentric
fragments produced by a single centromeric breakage event in maize. Poster presented at
Plant Biology Symposium, University of Minnesota Plant Biology Dept. October 25-27,
1989.

Staub, R.W. and K.L. Rose. Centromeric breakage and meiotic fragment disjunction in
Zea mays L. 1989. Genetics 122:s26.

Staub, R.W. and K.L. Rose. 1989. Centromeric breakage and meiotic fragment
disjunction in maize. Poster presented at annual Maize Genetics Conference, March 2-5,
1989.

Rose, K.L. and R.W. Staub. 1989. Centromeric breakage and meiotic fragment
disjunction in maize. Poster presented at Research in Life Sciences Mini-symposium,
Iowa State University, March 10-11, 1989.

Staub, R.W., D. Beckman, and E. Weck. 1988. Mu sequences in plants with B

chromosomes. Poster presented at annual Maize Genetics Conference, March, 1988.

Staub, R.W. and P.M. Laurenson. 1987. Consistent nondisjunction of B chromosomes in

Black Mexican. Maize Genetics Cooperative News Letter 61:80.

Staub, R.W. and P.M. Laurenson. 1987. Dosage analysis of the D8 allele for dwarfism.
Maize Genetics Cooperative News Letter 61:79-80.

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Staub, R.W. 1984. The effect of B chromosomes on repair of gamma radiation induced
DNA damage. Paper presented at annual Maize Genetics Conference, March, 1984.

Staub, R.W. 1983. Leaf striping in Black Mexican sweet corn with high numbers of B
chromosomes. Paper presented at annual Maize Genetics Conference, March, 1983.

Staub, R.W. 1982. Striated leaf in maize is related to the presence of B chromosomes.
Paper presented to the 26th Annual Meeting of the Arizona-Nevada Academy of Science,
April, 1982.

Staub, R.W. 1982. The effect of B chromosomes on the susceptibility of maize to gamma
radiation. Paper presented to the 58th Annual Meeting of the SWARM Division of the
AAAS, April, 1982.

Seminars/Workshops/Continuing Education

ASCLD 40th Annual Symposium: The Business Behind The Science. May 5 – 9, 2013.
Durham, NC.

Twenty-third International Symposium on Human Identification. Nashville, TN.

October 15-19, 2012.

Presented Continuing Education Session to Orchid Cellmark forensic analysts: A Study

of the Effects of Database Size and Loci Number on the Potential for Successful Familial
Searches in Databases of STR Profiles. Dallas, TX. March 7, 2012.

American Academy of Forensic Sciences 64th Annual Scientific Meeting. Atlanta, GA.
February 20th – 25th, 2012.

Overview of CODIS and National DNA Index System. 1.5 hour presentation by Mollie
Bride at Orchid Cellmark on December 13, 2011.

Twenty-second International Symposium on Human Identification (including Familial

Search Workshop). National Harbor, MD. October 3rd – 6th, 2011.

American Academy of Forensic Sciences 63rd Annual Meeting. Chicago, IL. February
21st-26th, 2011 ( including 2/22/11 DNA Mixture Workshop).

Twenty-first International Symposium on Human Identification (including Familial

Search Workshop). San Antonio, TX. October 11th – 14th, 2010.

Instructor for Relationship Testing Workshop at Illinois State Police Laboratory in

Chicago, IL. September 21st – 22nd, 2010.

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Association of Forensic DNA Analysts and Administrators Summer Meeting. Austin,
TX. July 8th – 9th, 2010.

American Academy of Forensic Sciences 62nd Annual Meeting. Seattle, WA. February
22th – 27th, 2010.

Association of Forensic DNA Analysts and Administrators Winter Meeting. Austin, TX.
January 28th – 29th, 2010.

Twentieth International Symposium on Human Identification. Las Vegas, NV. October

12th – 15th, 2009.

Association of Forensic DNA Analysts and Administrators Summer Meeting. Austin,

TX. August 16th – 17th, 2009.

Applied Biosystems HID University Future Trends in Forensic DNA Technology

Seminar Series – Austin, TX. August 15, 2009.

Fundamentals of Capillary Electrophoresis & Maintenance and Troubleshooting of

3100/3130XL Platforms. A workshop presented by Dr. Aaron LeFebvre at Orchid
Cellmark. Dallas, TX. February 13th, 2009.

Troubleshooting Common Laboratory Problems – Workshop at Nineteenth International

Symposium on Human Identification. Hollywood, CA. October 16, 2008.

Nineteenth International Symposium on Human Identification. Hollywood, CA. October

13 – October 16, 2008.

Eighteenth International Symposium on Human Identification. Hollywood, CA.

September 30 – October 4, 2007.

59th Annual Meeting of the American Academy of Forensic Sciences. San Antonio, TX.
February 19-24, 2007.

Seventeenth International Symposium on Human Identification. Nashville, TN. October

9-12, 2006.

2nd Annual Present and Future Technological Advances in Human Identification

Conference sponsored by The Virginia Institute of Forensic Science and Medicine.
Roanoke, VA. March 27 - 29, 2006.

Evaluation of Expert Systems for STR Genotyping Applications Workshop at 2nd Annual
Present and Future Technological advances in Human Identification Conference
sponsored by The Virginia Institute of Forensic Science and Medicine. Roanoke, VA.
March 27, 2006.

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Sixteenth International Symposium on Human Identification. Grapevine, TX.
September 25-29, 2005.

DNA Auditor Training. Presented by the FBI Laboratory. Sponsored by Tennessee

Bureau of Investigation. May 11-12, 2005. Nashville, TN.

57th Annual Meeting of the American Academy of Forensic Sciences. New Orleans, LA.
February 21-26, 2005.

Y-STRs: Practical Considerations and Interpretation Issues Workshop. Phoenix, AZ.

October 7, 2004.

Fifteenth International Symposium on Human Identification. Phoenix, AZ. October 5 –

7, 2004.

Forensic Human Mitochondrial DNA Analysis Workshop. AAFS. Dallas, TX. February
16, 2004.

56th Annual Meeting of the American Academy of Forensic Sciences. Dallas, TX.
February 16-21, 2004.

Fourteenth International Symposium on Human Identification. Phoenix, AZ. September 30 –

October 2, 2003.

Forensic Applications of Y-Chromosome DNA Typing Symposium. Hosted by the

Tennessee Bureau of Investigation Forensic Sciences Division. Nashville, TN. May 12,
2003.

George Carmody, Ph.D. – Statistical Analysis of Forensic DNA Evidence workshop.

Orchid Cellmark. April 28-30, 2003.

55th Annual Meeting of the American Academy of Forensic Sciences. Chicago, IL.
February 17-22, 2003.

Thirteenth International Symposium on Human Identification, Phoenix, AZ, October 7-10, 2002.

54th Annual Meeting of the American Academy of Forensic Sciences. Atlanta, GA. February 11-
16, 2002.

Twelfth International Symposium on Human Identification, Biloxi, MS, October 10 - 13, 2001.

53rd Annual Meeting of the American Academy of Forensic Sciences. Seattle, WA. February 19-
24, 2001.

Eleventh International Symposium on Human Identification, Biloxi, MS, October 10 - 13, 2000.

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52nd Annual Meeting of the American Academy of Forensic Sciences. Reno, NV. February 21-
26, 2000.

Ninth International Symposium on Human Identification, Orlando, FL, October 8-10,

1998.

Expert Witness Workshop, Ninth International Symposium on Human Identification,

Orlando, FL, October 6, 1998.

Parentage Testing Special Interest Group Session, American Association of Blood Banks,
Denver, CO, October 18, 1997.

Eighth International Symposium on Human Identification, Scottsdale, AZ,

September 17-21, 1997.

Parentage Testing Forum, American Association of Blood Banks, Orlando, FL. October
15, 1996.

Seventh International Symposium on Human Identification, Scottsdale, AZ,

September 19-21, 1996.

Parentage Testing Forum, American Association of Blood Banks, New Orleans, LA,
November 14, 1995.

Sixth International Symposium on Human Identification, Scottsdale, AZ,

October 12-14, 1995.

Parentage Testing Forum, American Association of Blood Banks, San Diego, CA,
November 15, 1994.

Fifth International Symposium on Human Identification, Scottsdale, AZ,

October 8-11, 1994.

Fourth International Symposium on Human Identification, Scottsdale, AZ,

September 25-30, 1993.

Introduction to PCR, Biotechnology Training Programs, Inc., Duke University, Durham,

NC, May 19, 1993.

Forensic Statistics. Arizona State University College of Law, Tempe, AZ,

March 18 - 21, 1993.

Third International Symposium on Human Identification, Scottsdale, AZ,

April 30 - May 2, 1992.

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Parentage Testing Forum, American Association of Blood Banks, Baltimore, MD,
November 12, 1991.

Second International Symposium on Human Identification, Madison, WI,

April 10 - 11, 1991.

Human Genome Project and Polymerase Chain Reaction (PCR) Problems. North
Carolina Biotechnology Center. North Carolina Agricultural and Technical State
University, Greensboro, NC, November 29, 1990.

American Society of Human Genetics, Annual Meeting, Cincinnati, OH,

October 18 - 19, 1990.

Curriculum Vitae
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 Exhibit 2 of the Report of Rick W. Staub, Ph.D.
Prosecution Testing
Date State Court Case # or Defense Type/Offense performed
Middlesex
1/11/2010 MA Superior Court MA08-0353 P Incest STR
Genesee County
7/23/2010 MI Circuit Court MF09-0001 P Murder x2 MiniFiler
Superior Court of
9/30/2010 WA Washington WA08-0020 P Sexual Assault STR, Y-STR
3/11/2011 IL CA98-025515 P Homicide STR
FOR4721/FR07-
3/28/2011 GA 0037/FR07-0171 P Homicide STR
3/31/2011 WA WA07-0011 P Homicide Y-STR
8th Judicial Sexual Assault
8/24/2011 KY Circuit FK10-0003 P of Minor STR
6th Judicial Sexual Assault
9/1/2011 SD Circuit Y08-0111 P of Minor Y-STR
Cook County
Criminal Court
9/14/2011 IL District 1 IL07-0306 P Homicide STR
Middlesex Sexual Assault
10/21/2011 MA Superior Court MA10-0053 P of a Minor STR
Monroe County Incest / SA of
10/25/2011 IN Circuit Court FR10-0184 P minor STR
11/8/2011 NY Federal FR11-0128 P Identity theft? STR
Aggrevated
assault?/Bombi Minifiler (first
1/19/2012 AZ U.S. District FR07-0191 P ng case)
Possession of
4/10-4/12/12 CA US. District CR12-0012 P firearm by felon STR
296th Judicial Aggrevated
4/18/2012 TX District Court FR05-0074 P Sexual Assault STR, Y-STR
4/20/2012 ND FR11-0067 P Murder Y-STR
Cumberland
County Superior
6/13/2012 ME Court Y08-0035 D Murder Y-STR, STR
240th District 5194 / FO05-
7/23/2012 TX Court 0009 P Murder STR
Summit County Y-STR,
10/22//2012 OH Common Pleas CR 1998-02-0463 D Murder MiniFiler
Manslaughter,
Criminal
negligence
causing death,
Unlawful
confinement
Supreme Court of 29875-2/M06- and Mitochondrial
1/22/2013 BC British Columbia 0022 P imprisonment sequencing
MiniFiler
Agg. Sex. Ass. (HPD ran
176th Criminal Of and Elderly standard
9/23/2013 TX MF11-0007 P
Court Person/Homicid STRs on
e other
samples
Worcester Trial
1/28/2014 MA Court MA09-0160 P Murder Identifiler
 Court of Common
3/12/2014 OH Pleas D Murder None
 Exhibit 3 of the Report of Rick W. Staub, Ph.D.
State Bates
Description Date Start Bates End Bates Range
CPD Supplemental
Report 8/24/1984 OHP00001985 OHP00001986 6692-3
Report of Autopsy 8/25/1984 CCMEO00000699 CCMEO00000703 N/A
Photo 8/25/1984 CCMEO00000910 CCMEO00000910 N/A
Autopsy Photo 8/25/1984 CCMEO00000974 CCMEO00000974 N/A
Coroner's Verdict 8/24/1984 CCMEO00000706 CCMEO00000706 N/A
1984 Standard Diary 8/25/1984 CCMEO00000511 CCMEO00000517 N/A
Handwritten notes 8/25/1984 OHP00000527 OHP00000537 5224-5234
Handwritten notes 8/25/1984 CCMEO00000502 CCMEO00000510 N/A
Record of Specimen
Submitted by
Pathology 8/25/1984 CCMEO00000570 CCMEO00000592 N/A

Report from
Laboratory Trace
Evidence Department
Re: Mary Ann Flynn 8/25/1984 CCMEO00000645 CCMEO00000652 N/A
Blood Group
Determination 8/28/1984 CCMEO00000593 CCMEO00000593 N/A
CPD Supplemental 8/25/1984-
Report 9/19/84 OHP00006051 OHP00006126 538-613
Photos ?/?/1984 OHP00009398 OHP00009405 N/A
Pathology Report 8/30/1984 CCMEO00000656 CCMEO00000656 N/A
CPD Supplemental
Report 10/4/1984 OHP00005785 OHP00005785 4500
Letter to FBI 9/7/1984 OHP00006115 OHP00006117 602-604
Dr. Balraj Report 10/15/1984 CCMEO00000842 CCMEO00000842 N/A
Notice of Alibi 11/24/1984 OHP00005139 OHP00005140 3854-5
Trial Transcripts OHP00006854 OHP00009395 1-2523
Affidavit 5/20/1988 OHP00000393 OHP00000394 5090-1
Affidavit 5/1/1988 OHP00005323 OHP00005324 4038-9
Handwritten Notes 5/1/1988 OHP00005835 OHP00005837 4550-2
Affidavit 5/17/1988 OHP00005325 OHP00005326 4040-1
Annotated Trace
Evidence Report ?/?/1988 OHP00005848 OHP00005855 4563-70
Handwritten Notes ?/?/1988 OHP00005822 OHP00005822 4537
Affidavit 5/18/1988 OHP00002646 OHP000002647 1787-8
Affidavit 5/25/1988 OHP00003975 OHP00003993 3116-3134
Affidavit 7/29/1988 OHP00004822 OHP00004831 3537-46
Motion to Compel
Shelton DNA 2/21/1989 OHP00005295 OHP00005398 4010-13

Coroner’s Office Letter 4/20/1992 OHP00000539 OHP00000539 5236

Letter 5/21/1992 OHP00005268 OHP00005268 3983
Letter 8/19/1995 OHP00004447 OHP00004482 3162-3197
Counsel Notes 9/29/1995 OHP00003265 OHP00003266 2406-7
Handwritten note 2/4/2000 OHP00000002 OHP00000002 4657
Handwritten Notes 3/2/2000 OHP00005150 OHP00005152 3865-7
STR Manual 2000 7/1/2000 CCMEO00000198 CCMEO00000310 N/A
STR Manual 2001 7/1/2000 CCMEO00000311 CCMEO00000449 N/A
Letter 9/1/2000 CCMEO00000667 CCMEO00000667 N/A
Dr. Balraj Report 9/25/2000 CCMEO00000597 CCMEO00000602 N/A
Log 11/30/2000 CCMEO00000193 CCMEO00000197 N/A
Dr. Balraj Report 3/7/2001 CCMEO00000605 CCMEO00000605 N/A
Laboratory notes 1/1/2001 OHP00000156 OHP00000207 4811-4862
Laboratory 11/16/2000,
Examination Report 3/7/2001 CCMEO00000046 CCMEO00000048 N/A
Proficiency Records 3/8/2001 CCMEO00000134 CCMEO00000137 N/A
Log 6/1/2001 CCMEO00000160 CCMEO00000162 N/A
Log 6/4/2001 CCMEO00000171 CCMEO00000172 N/A
Laboratory notes 10/18/2001 CCMEO00000050 CCMEO00000133 N/A
Laboratory notes 10/18/2001 OHP00000068 OHP00000155 4723-4810
Genotyper Result 9/6/2008 CCMEO00000006 CCMEO00000006 N/A
2006 CCMEO
Documents 3/26/2006 CCMEO00000029 CCMEO00000032 N/A

Lists of Items Retained

By Trace
Evidence/DNA
Department for
Coroner's Case 190729 1/4/2007 CCMEO00000016 CCMEO00000020 N/A
Laboratory notes 12/4/2008 CCMEO00000004 CCMEO00000004 N/A
Communications
Record 12/10/2008 CCMEO00000003 CCMEO00000003 N/A
Handwritten Notes 2/5/2009 CCMEO00000567 CCMEO00000567 N/A

Letter and Balraj

Deposition Transcript 2/16/2009 CCMEO00000715 CCMEO00000742 N/A
Email 8/20/2013 BCII00000102 BCII00000102 N/A
Email attachment 8/20/2013 BCII00000103 BCII00000115 N/A
Email attachment 8/20/2013 BCII00000116 BCII00000116 N/A
Email attachment 8/20/2013 BCII00000117 BCII00000120 N/A
Handwritten Notes Undated OHP00005884 OHP00005884 4599
Handwritten note Undated OHP00000595 OHP00000595 5292
Handwritten note Undated OHP00000596 OHP00000596 5293
DNA Sample of Ronnie
Shelton from Ohio
Convicted Offender
Database Undated BCII BCII N/A
Exhibit 4 of the Report of Rick W. Staub, Ph.D.

1. NIST’s STRbase website: http://www.cstl.nist.gov/strbase/

2. National Research Council. (1992) DNA Technology in Forensic Science, National Academy

Press, Washington, D.C., 185 pages

3. National Research Council. (1996) The Evaluation of Forensic DNA Evidence. National

Academy Press, Washington, D.C., 254 pages

4. Butler, J.M. (2012) Advanced Topics in Forensic DNA Typing: Methodology. Elsevier

Academic Press, San Diego, 704 pages

Exhibit 5: Allele Summary Chart

ALLELES DETECTED - PROFILER PLUS

Sample D3S1358 vWA FGA AMEL D8S1179 D21S11 D18S51 D5S818 D13S317 D7S820
Mary Ann Flynn Reference 16,18 16,18 20,23 X 10,13 28,31.2 17,18 11,13 12 10,11
Anthony Apanovitch Reference 14,17 14,15 23,26 X,Y 10,13 30,31 17,18 11 11,14 11,13
1.1 EF 16,18 16*,18* NR X 10,13 28*,? NR 11,13* 12*,? NR
1.1 MF NR NR NR NR NR NR NR NR NR NR
1.2 EF 16,18 16*,? NR X 10,13 28*,? NR 11,13 NR NR
1.2 MF Deduced major male 16,17 16,18 23,25 X,Y 10,13 30,32.2 15 12 8 10,12
1.2 MF Likely minor profile assuming two 2 of 31.2*, 2 of 8*; 8*,10;
15*,18* 14*,15* 22*,? X or X,Y 13,14 13*,14* 8,11* or 11*
contributors 27*,28*,29* 10*,11*,13* or 8*,12
2.1 EF 14*,16* 15*,18* 23*,? X 13*,14,15 28*,30* NR 13 12*,13* 8*,?
2.1MF NR NR NR NR NR NR NR NR NR NR
3.1 EF NR NR NR X NR NR NR 11* NR 5*
3.1 MF NR NR NR NR NR NR NR NR NR NR
6.1EF NR NR NR X* NR NR NR 13* NR NR
6.1 MF 14*,17* NR NR X,Y 10*,13* NR NR 11* NR NR

EF = epithelial cell fraction or non-sperm fraction

MF = male fraction or sperm fraction
NR = no result
* = allele below CCMEO RFU calling threshold
? = any possible allele due to possible stochastic event