Scribd Logo
Upload

Welcome to Scribd!

  • 37 views

    Methods and Results Microscope

    Uploaded by

    marylen martin
    The document describes procedures for using a compound microscope, including: 1) Identifying the major parts of the microscope and their functions. 2) Focusing the microscope by centering the sample, adjusting lighting and using coarse and fine focus knobs to sharpen the image at 4x and 10x magnifications. 3) Measuring the diameter of the field of view at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Methods and Results Microscope

    Uploaded by

    marylen martin
    37 views4 pages
    The document describes procedures for using a compound microscope, including: 1) Identifying the major parts of the microscope and their functions. 2) Focusing the microscope by centering the sample, adjusting lighting and using coarse and fine focus knobs to sharpen the image at 4x and 10x magnifications. 3) Measuring the diameter of the field of view at different magnifications.

    Original Description:

    lab report biology

    Original Title

    Methods and results microscope

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document describes procedures for using a compound microscope, including: 1) Identifying the major parts of the microscope and their functions. 2) Focusing the microscope by centering the sample, adjusting lighting and using coarse and fine focus knobs to sharpen the image at 4x and 10x magnifications. 3) Measuring the diameter of the field of view at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    37 views4 pages

    Methods and Results Microscope

    Uploaded by

    marylen martin
    The document describes procedures for using a compound microscope, including: 1) Identifying the major parts of the microscope and their functions. 2) Focusing the microscope by centering the sample, adjusting lighting and using coarse and fine focus knobs to sharpen the image at 4x and 10x magnifications. 3) Measuring the diameter of the field of view at different magnifications.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Methods

    A. Identification of Parts

    Compound microscope was obtained from the cabinet and it was placed securely on
    the table. The following parts on the microscope was identified , and was labeled in
    Figure 1.

    B. Focusing the Microscope

    1. The nosepiece was turned so that the lowest power lens in straight alignment
    over the stage .
    2. Focusing was began with the lowest power objective lens (4x).
    3. A slide of letter ‘e’ was placed on the stage and was stabilized with the clips .The
    ‘e’ was centered .
    4. The distance between the stage and the tip of the objective lens was
    decreased .
    5. The diaphragm was rotated to give the maximum amount of light .
    6. The distance between the stage and the object lens was increased using the
    coarse adjustment knob until the object comes into view .
    7. The fine adjustment knob was used to sharpen the focus.
    8. Steps 1 to 7 was repeated by changing the power lens to 10x.

    C. Measuring the diameter of the field of view

    1. The nosepiece was turned so that the lowest power lens in straight line
    alignment over the stage.
    2. The light was adjusted and saw a uniformly bright field of view .
    3. Transparent ruler was put on stage.
    4. The field of view diameter was measured.
    Results

    A. Identification of Parts
    Eyepiece

    Body type

    Nose piece

    Objective lens
    Arm

    Mechanical stage
    Stage clips
    Stage
    Diaphragm Mechanical stage control knob
    m
    condenser Coarse adjustment knob

    Light source Fine adjustment knob

    Base

    Figure 1 : Compound microscope

    Table 1 : Component of microscope and function


    Parts Function
    Eyepiece Topmost series of lenses through which
    an object is viewed.
    B.
    Body tube Conduct light rays
    Arm Supports upper parts and provides
    carrying handle
    Nosepiece Revolving device that holds objectives.
    Objective lenses These are major lenses used for image
    image formation
    Coarse-adjustment knob Knob used to bring object into
    approximate focus
    Fine - adjustment knob Knob used to bring object into final focus
    Diaphragm Control the amount of illumination used
    to view the object
    Light source An attached lamp that directs a beam of
    light up through to view the object
    Base The flat surface of the microscope that
    rests on the table
    Stage Holds and supports microscope slides
    Stage clips Hold slides in place on the stage
    Mechanical stage A moveable stage used in accurate
    positioning of the slide.
    Mechanical stage control knob 2 knobs that are usually located below
    the stage. One knob control
    forward/reverse movement, and the
    other controls the right/left movement.

    Focusing the Microscope

    Table 2 : The image of letter ‘e’ under different magnification


    Magnification Letter ‘e’
    4x

    10x

    40x

    C. Measuring the diameter of the field of view

    Magnification Length (mm)


    4x 5
    10x 2

    You might also like