General

 Lab  Safety                  
Procedure    
 

   
Purpose
 
The  purpose  of  this  procedure  is  to  support  work  practices  for  protecting  laboratory  personnel  from  
potential  health  hazards  in  the  laboratory.    
 
1. Laboratory Safety Guidelines    
1.1  GENERAL  LABORATORY  SAFETY  
 

• Do  not  eat,  drink,  or  apply  cosmetics  in  the  lab.    

• Store  food  and  drink  in  food  designated  refrigerators  only.  Don’t  mix  chemicals  and  food.      
• Tie  back  medium  length  and  long  hair  when  working  near  flames  or  entangling  equipment.    
• All  accidents,  no  matter  how  minor,  should  be  reported  to  the  faculty/staff  member  supervising  
the  laboratory.  
• Know  the  location  of  all  safety  equipment  (e.g.  eyewash,  fire  extinguisher,  fire  blanket,  safety  
showers,  spill  kit)  if  available.      
• Keep  aisles  clear.    
• Maintain  unobstructed  access  to  all  exits,  fire  extinguishers,  electrical  panels,  emergency  
showers,  and  eyewashes.    
• Do  not  use  corridors  for  storage  or  work  areas.    
• Do  not  store  heavy  items  above  6  feet  high.    
• Consult  a  coworker  if  planning  to  work  alone  or  running  an  unattended  operation.  
• Never  work  alone  in  the  chemistry  lab  or  when  performing  high-­‐risk  operations.  
• Keep  area  clean  and  uncluttered;  clean  up  area  upon  completion  of  task  or  at  end  of  the  day.    

1.2  PERSONAL  PROTECTIVE  EQUIPMENT  (PPE)  

• Review  SOP,  MSDS  and  other  hazard  information  to  determine  appropriate  PPE  to  wear  based  
on  chemical  hazards  encountered.  
• Lab  coats  and  safety  glasses  are  to  be  worn  at  all  times  in  the  chemistry  lab  and  when  using  
hazardous  chemicals.  
• Remove  gloves  when  leaving  the  laboratory,  so  as  not  to  contaminate  doorknobs,  etc.    

1.3  ELECTRICAL  SAFETY  

• Don’t  use  permanent  extension  cords.      

1.4  CHEMICAL  SAFETY  

• Know  the  hazards  of  the  chemicals  you’re  working  with.  Consult  the  material  safety  data  sheet  
(MSDS)  or  other  appropriate  references  prior  to  using  a  chemical  with  which  you’re  unfamiliar.  
• Make  sure  all  chemicals  are  clearly  and  currently  labeled  with  the  substance  name.    
• Use  volatile  and  flammable  compounds  only  in  a  fume  hood.  Procedures  that  produce  aerosols  
should  be  performed  in  a  hood  to  prevent  inhalation  of  hazardous  material.  Be  sure  the  fan  is  on  
at  all  times  when  using  a  fume  hood.  Fume  hoods  should  not  be  used  for  storage.    
• Material  Safety  Data  Sheets  (MSDS)  shall  be  provided  for  all  hazardous  chemicals  before  use.    
 • Keep  proper  records  of  time  sensitive  chemicals  (oxidizers,  THF,  and  organic  peroxides),  and  
dispose  of  all  these  chemicals  before  their  expiration  date.  
• Perform  proper  housecleaning  of  your  lab  area  once  a  year  to  discard  of  unused  chemicals  and  
materials.    General  chemicals  that  have  been  around  for  three  years  or  more  should  be  
discarded.    
• Provide  a  check  in  procedure  for  incoming  researchers  and  visiting  researchers.    Review  safety  
and  operational  procedures  with  them.    
•  If  a  researcher  will  be  leaving  Caltech,  please  go  through  a  check  out  procedure  with  your  
researcher  (visiting  and  otherwise)  that  all  chemicals  and  related  materials  (desiccants,  silica  
filtration  beads,  etc.)  are  also  properly  disposed  of,  prior  to  them  leaving  Caltech.    This  helps  to  
avoid  any  unknowns  in  your  lab  area,  which  are  difficult  to  manage  by  the  Institute.  
• Clean  up  of  large  spills  should  not  be  attempted.  Call  Environmental  Health  and  Safety  Office  at  
6727  for  clean  up.      

1.5  FLAMMABLE  AND  COMBUSTIBLE  LIQUIDS  

• Use  fire-­‐hazard  chemicals  in  vented  hoods  and  away  from  sources  of  ignition.  Fire-­‐hazard  
chemicals  are  chemicals  with  a  flash  point  below  200°  F  (93.3°  C).    
• Store  flammable  liquids  in  excess  of  10  gallons  in  approved  flammable  liquid  storage  cabinets.    
• Follow  proper  storage  procedures  for  flammable  and  combustible  liquids.    This  includes  not  
storing  corrosives  and  flammables  in  the  same  cabinet.  

1.6  OLD,  DAMAGED,  OR  NO  LONGER  USEFUL  EQUIPMENT    

• Review  the  equipment  asset  list  for  your  lab  at  least  once  a  year  to  see  if  you  have  old,  
damaged,  or  outdated  equipment  that  require  disposal.  
• For  proper  disposal,  notify  the  Safety  Office  if  you  believe  that  the  equipment  has  been  used  for  
radiological,  biological,  or  chemical  work.    The  types  of  equipment  should  be  decontaminated  
and  certified  prior  to  disposal,  and  the  Safety  Office  is  able  to  assist  you  with  this  process.  
• Contact  your  Building  Administrator  if  you  have  a  large  amount  of  e-­‐waste  for  disposal,  as  they  
work  with  you  to  get  this  matter  taken  care  of,  or  you  may  take  it  over  to  the  Recycling  Center  
on  the  first  Wednesday  of  each  month  between  9:00am  and  12:00pm  for  proper  disposal.  
• Do  not  leave  old  equipment  in  the  hallways,  as  these  are  potential  fire  hazards.  

1.7  EMERGENCY  RESPONSE  PROCEDURES    

• Emergencies  on  campus  (ex:  police,  fire,  paramedics,  chemical,  etc.)  CALL  5000.  

1.7.1  Fire  

• Remain  calm  
• Alert  others  
• Close  doors    
• Evacuate  to  EAP  (Gates  courtyard)  
 1.7.2  Earthquake    

• Remain  calm  
• Drop,  cover,  and  hold  
• Evacuate  when  shaking  stops  if  building  damage  present    

1.7.3  Shelter  in  Place  when  

• A  chemical  or  biological  spill  elsewhere  on  campus  

• Severe  weather    
• Or  an  armed  individual  on  campus    

1.7.4  Biological,  Chemical,  and  Radiological  Incidents    

• Attend  to  injured  persons    

• Confine  the  area    
• Get  help-­‐notify  Safety  or  Security  
• Evacuate  if  necessary    

1.7.5  Personal  Injury    

• Check  the  area  for  additional  hazards  

• Call  5000  and  notify  the  supervisor  
• Care:  
• Remove  the  injured/exposed  individual  from  the  area,  unless  it  is  unsafe  to  do  so  because  of  the  
medical  condition  of  the  victim  or  the  potential  hazard  to  rescuers.  
•  Report  the  exposure  to  EH&S.  
• Flush  contamination  from  eyes/skin  using  the  nearest  emergency  eyewash/shower  for  a  
minimum  of  15  minutes.  Remove  any  contaminated  clothing.    
• Bring  to  the  hospital  copies  of  MSDSs  for  all  chemicals  the  victim  was  exposed  to.  

 
  

Lab  Specific  Procedure    

 

       
 
                         
2. Laboratory Specific Safety Procedure  
2.1GUIDELINES  FOR  USING  NANOMATERIALS  
Exposure  standards  have  not  been  established  for  engineered  nanoparticles  in  the  United  States  or  
internationally  [Safe  Nanotechnology  2008.]  Therefore,  the  guidelines  below  are  designed  to  help  
researchers  implement  a  combination  of  engineering  controls,  work  practices,  and  personal  protective  
equipment  to  minimize  potential  exposures  to  themselves  and  others.      

As  a  general  and  quick  guide  to  the  exposure  risks  and  prudent  control  measures  to  be  used  for  
common  laboratory  operations  involving  nanomaterials,  refer  to  the  table  below.  It  is  important  to  
consider  if  the  nanoparticles  are  in  an  agglomerated  or  aggregated  form,  functionalized,  suspended  in  
liquid,  or  bound,  as  these  conditions  may  affect  the  exposure  potential.  

Quick  Guide:  Exposure  Risks  and  Control  Measures  for  Common  Laboratory  Operations  Involving  
Nanomaterials  

Activity  types,  by  Risk  of  Exposure   Primary  Control  Measures  

Low  Probability:     • Disposable  nitrile  or  PVC  gloves.  Do  not  
reuse  gloves.  
• Non-­‐destructive  handling  of  solid  
nanoparticle  composites  or  nanoparticles   • Wet  cleaning  procedures  and/or  HEPA  
permanently  bonded  to  a  substrate     vacuum  for  surfaces/equipment.  

Medium  /  High  Probability:     • Conduct  task  within  a  fully  enclosed  

system  (e.g.,  glove  box),  or  fume  hood    
• Working  w/  nanomaterials  in  liquid  media   • Disposable  gloves  appropriate  for  the  
during  pouring  or  mixing,  or  where  a  high   solvent  in  which  the  particles  are  
degree  of  agitation  is  involved  (e.g.,   suspended.  Do  not  reuse  gloves.    
sonication)     • Safety  eyewear  (+  face  shield  if  splash  
• Handling  nanostructured  powders*     potential  exists)    
• High  speed  abrading/grinding  nano-­‐ • Wet  cleaning  procedures  for  
composite  materials     surfaces/equipment  
• Maintenance  on  equipment  used  to  
produce  nanomaterials    
• Cleaning  of  dust  collection  systems  used  to  
capture  nanoparticles  

High  Probability:     • Work  in  enclosed  systems  only  (e.g.,  

glove  box,  glove  bag,  or  sealed  chamber).  
• Generating  nanoparticles  in  the  gas  phase  
or  in  aerosol  (spill  or  liquid)    
• Manipulation  of  nanoparticles  in  gas  stream  
  

2.2  COMPRESSED  GAS  CYLINDERS  

 
• For  all  lab  personnel:  
o  Should  be  familiar  with  the  gas  container  content  and  the  potential  hazards.  They  
should  have  access  to  the  appropriate  Material  Safety  Data  Sheet.    
o Secure  cylinders  at  the  top  and  bottom.  Keep  the  cylinder  capped  when  not  in  use.    
o All  gas  cylinders,  including  lecture  and  empty  bottles,  should  be  in  an  upright  manner  
and  chained.    
• For  lab  personnel  responsible  for  changing  out  compressed  gas  cylinders:    
o Wear  safety  glasses  when  handling  compressed  gases.    
o Do  not  lubricate,  modify,  force,  or  tamper  with  cylinder  valves.    
o Always  make  sure  that  the  regulator  appears  sound  before  attaching  it  to  a  cylinder.    
o Make  sure  that  the  correct  regulator  and  CGA  connector  is  being  used.  See  CGA  
Connection  Chart  at  the  end  of  this  document.  If  the  connections  do  not  fit  together  
readily,  the  wrong  regulator  or  a  defective  regulator  is  probably  being  used.    
o Use  only  the  correct  fittings  and  connections  to  ensure  compatibility.  Make  sure  that  
the  threads  on  the  cylinder  and  the  connection  mate,  and  are  of  a  type  intended  for  gas  
service.    
o Attach  the  regulator  securely  with  the  secondary  valve  closed  and  preferably  with  the  
regulator  flow  backed  off  (counterclockwise)  before  opening  the  cylinder  valve  wide.    
o When  cylinders  are  no  longer  in  use,  shut  the  valves,  relieve  the  pressure  in  the  gas  
regulators,  remove  the  regulators  and  cap  the  cylinders.    
o Before  returning  empty  gas  containers,  a  check  should  be  carried  out  to  ensure  that  the  
container  valve  is  closed  (and  not  leaking)  and  that  the  valve  outlet  plug  (or  cap  nut)  has  
been  securely  refitted.    
o Leave  a  small  amount  of  contents  in  the  cylinder  to  avoid  contamination.    
o Segregate  gas  cylinder  storage  from  chemical  storage.    
o Keep  incompatible  classes  of  gases  stored  separately.  Keep  flammables  from  reactives  
which  include  oxidizers  and  corrosives.  For  example,  keep  cylinders  containing  oxygen  
or  oxidizing  gases  away  from  flammable  solvents,  combustible  materials,  unprotected  
electrical  connections,  gas  flames  or  other  sources  of  ignition.    
o Always  label  cylinders  so  you  know  their  contents;  do  not  depend  on  the  manufacturers  
color  code.  Gas  cabinets  should  have  a  clear  label  on  the  outside.    
o Note  the  name  and  phone  number  of  the  supplier  of  the  cylinder.    Cylinders  are  
generally  “loaned”  when  they  are  distributed,  and  the  empty  cylinders  are  to  be  
returned  to  the  supplier  once  you  are  finished  with  the  gas.  
o If  a  cylinder  of  material  has  been  here  for  more  than  three  years,  please  contact  the  EHS  
Office  and  mark  it  for  return  to  the  supplier.  

2.3  CORROSIVE  AND  CONTACT-­‐HAZARD  MATERIALS      

 
Corrosive,  allergenic,  and  sensitizer  information  is  given  in  MSDS  and  on  chemical  container  labels.    
Handling  processes  should  be  designed  to  minimize  the  potential  for  splash,  splatter,  or  other  likely  
scenarios  for  accidental  contact.  Handle  corrosive  chemicals  and  contact-­‐hazard  chemicals  with  all  
proper  safety  precautions  according  to  the  way  they  will  be  used.  This  may  include  wearing  both  safety  
goggles  and  face  shield,  gloves  tested  for  absence  of  pinholes  and  known  to  be  resistant  to  permeation  
or  penetration,  and  a  laboratory  apron  or  laboratory  coat.  Additional  protective  clothing  (i.e.,  apron,  
over  sleeves)  is  appropriate  where  chemical  contact  with  body  and/or  skin  is  foreseeable.  

• Do  not  pour  water  into  acid.  Slowly  add  the  acid  to  the  water  and  stir.  
• Open  bottles  or  carboys  slowly  and  carefully  and  wear  protective  equipment  to  guard  hands,  
face,  and  body  from  splashes,  vapors,  gases  and  fumes.  
• Use  a  mechanical  aid  or  a  pipette  bulb  for  pipetting.    
• Wipe  drips  from  containers  and  bench  tops.  Be  especially  careful  to  wipe  up  visible  residues  of  
sodium  hydroxide  and  potassium  hydroxide  from  all  surfaces.  Skin  contact  with  dry  residue  will  
result  in  burns.  
• Strong  acids/bases  are  to  be  handled  in  a  fume  hood.  
• Corrosives  should  never  be  stored  above  eye  level.    

2.4  PHOSPHORIC  ACID  

 
• Reacts  with  metals  to  liberate  flammable  hydrogen  gas.    
• Incompatible  with  sodium  tetrahydroborate  producing  a  violent  exothermic  reaction.    
• Heat  generated  with:  alcohols,  glycols,  aldehydes,  amides,  amines,  azo-­‐compounds,  carbonates,  
caustics,  esters,  ketones,  phenols  and  cresols,  organophosphates,  epoxides,  combustible  
materials,  unsaturated  halides,  organic  peroxides.    
• Formation  of  flammable  gases,  with  aldehydes,  cyanides,  mercaptins,  and  sulfides.    
• Formation  of  toxic  fumes  with  cyanides,  fluorides,  halogenated  organics,  sulfides,  and  organic  
peroxides.    
• Do  not  mix  with  solutions  containing  bleach  or  ammonia.    
• Incompatible  with  nitromethane,  chlorides  +  stainless  steel.    

2.5  HYDROCHLORIC  ACID  

 
• Incompatible  with  hydroxides,  amines,  alkalis,  cyanides,  sulfides,  sulfites,  formaldehyde,  copper,  
brass,  and  zinc.    
• Hydrochloric  acid  solution  in  water  is  a  strong  acid:  it  reacts  violently  with  bases  and  is  corrosive.  
• Reacts  violently  with  oxidants  forming  toxic  chlorine  gas.  Attacks  many  metals  in  the  presence  of  
water,  forming  flammable/explosive  hydrogen  gas.    
• When  heated  to  decomposition,  emits  toxic  hydrogen  chloride  fumes  and  will  react  with  water  
or  steam  to  produce  heat  and  toxic  and  corrosive  fumes.  Thermal  oxidative  decomposition  
produces  toxic  chlorine  fumes  and  explosive  hydrogen  gas.  
• If  involved  in  a  fire  use  water  spray  and  neutralize  with  soda  ash  or  slaked  lime.  

2.6  FUME  HOODS  

• Ensure  the  fume  hood  is  labeled  with  a  certification  date  of  less  than  one-­‐year  prior.  
• Maintain  hood  sash  at  or  below  the  maximum  height  indicated  by  an  arrow  on  the  side  of  the  
fume  hood.  Close  the  hood  sash  when  not  working  in  the  hood.    
• Equipment  used  in  hoods  should  be  placed  securely  on  blocks  to  allow  air  to  flow  under  and  
around  the  equipment.      
• Keep  chemical  sources  and  equipment  at  least  six  inches  away  from  the  face  or  rear  of  the  hood.  
 • Don’t  store  equipment  and  chemicals  in  the  hood  to  avoid  dead  air  spaces  and  to  prevent  
blocking  back  baffles.  
• Visually  inspect  baffles  (openings  at  the  top  and  rear  of  the  hood)  to  be  sure  slots  are  open  and  
unobstructed.  
• All  electrical  devices  should  be  connected  outside  the  hood  to  avoid  sparks  that  may  ignite  a  
flammable  or  explosive  chemical.    
• Do  not  use  a  fume  hood  for  any  function  which  it  is  not  intended.  Certain  chemicals  or  reactions  
require  special  constructed  hoods.  Examples  are  perchloric  acid  or  high  pressure  reactions.  
• If  you  are  not  sure  if  there  is  sufficient  airflow  in  your  fume  hood  due  to  extra  equipment,  please  
contact  the  EHS  Office  and  we  will  perform  a  survey  for  you.  

2.7  LIQUID  NITROGEN        

 
The  selection  of  materials  to  be  used  with  cryogenics  is  important  because  of  the  changes  in  physical  
properties  of  materials  at  very  low  temperatures.  The  Dewar  flask  is  the  most  common  container  used  
for  storage  and  transfer  of  cryogenic  fluids.  When  using  the  Dewar,  follow  these  procedures:    

• Cover  the  Dewar  with  a  cap  that  allows  escape  of  built-­‐up  pressure  and  keeps  air  and  moisture  
out.    
• Transfer  cryogenic  liquids  from  large  Dewar  vessels  with  special  transfer  tubes  designed  for  the  
particular  application.    
• Tipping  or  tilting  to  pour  the  liquid  may  damage  large  Dewars.  
• Do  not  use  heat  guns  or  similar  equipment  to  warm  transfer  tubing  quickly  for  disconnection.    
• Handle  containers  carefully  to  protect  the  vacuum  insulation  system  of  Dewars.  
• Place  large  Dewars  on  dollies  that  move  freely  so  there  is  no  possibility  of  personal  injury  or  
damage  to  the  supported  Dewars.  
• Due  to  extremely  cold  temperatures  of  cryogenic  liquids  and  “boil-­‐off”  gases,  use  the  following  
personal  protective  equipment  (PPE):  
o When  cryogenics  are  present,  safety  glasses  with  side  shields    
o When  cryogenics  are  poured  or  transferred,    
§ Safety  glasses  and  a  full  face  shield  
§ Loose-­‐fitting  thermal  gloves    
§ Long-­‐sleeved  clothing  (lab  coat)    
§ Long  pants    
§ Closed-­‐toe  shoes    
 
Anyone  using  cryogenic  material  must  receive  instruction  in  using  cryogenic  materials  safely  from  their  
lab  supervisor  or  safety  officer.  

If  there  is  a  cryogenic  spill,  immediately  leave  the  area.  If  you  believe  the  cryogen  has  caused  significant  
oxygen  depletion,  do  not  re-­‐enter  the  area  unless  the  oxygen  content  of  the  atmosphere  is  at  least  
19.5%  and  there  is  no  flammable  or  toxic  mixture  present.  

 
2.8 HYDROFLUORIC ACID

Hydrofluoric acid, a solution of hydrogen fluoride gas (HF) in water, is one of the most corrosive and
dangerous chemicals encountered in the laboratory. The following special safety precautions are
necessary when using this chemical, regardless if using dilute or concentrated HF.

2.8.1 First Aid Procedures and First Aid Kits

In the event of HF exposure, immediately start the first aid procedures as outlined in the Hydrofluoric
Acid First Aid Instructions below. Once first aid has been started, call 5000 to transfer person to
emergency room.

Post Caltech’s Hydrofluoric Acid First Aid Instructions in labs that keep or use HF gas or solutions;
see attached HF first aid instructions.

2.8.2 Personnel Exposures

o See the attached Hydrofluoric Acid First Aid Instructions for personnel exposures.

2.8.3 First Aid Kit

First aid kit must contain calcium gluconate gel for use in emergencies.

o 2.5% calcium gluconate gel can be purchased through many lab safety supply vendors.
o Ensure gel is intended for HF “dermal exposures” and has an effective shelf life of at least one
year.
o Create a system to refresh your supply of gel before the expiration date.

2.8.4 Personal Protective Equipment (PPE)

o Wear appropriate PPE, which includes:

o Goggles and face shield

o Butyl rubber or neoprene gloves
o Lab coat and closed-toe shoes

2.8.5 Safe Work Practices

o Work in a fume hood with the sash opening minimized—sash must not be opened beyond the
stickered arrow.
o Storage of HF and HF waste should be in a closed, labeled, chemically compatible container (e.g.
polyethylene or Teflon). Glass, metal, and ceramic containers are not compatible with HF. It
should have a polyethylene secondary containment tray.
o Avoid using glass containers. HF will etch and degrade glass.
o Use smallest quantities necessary.
o Check PPE for damage before using.
o HF  waste  should  be  placed  in  a  chemically  compatible  container  with  a  sealed  lid  and  clearly  
labeled.  
 2.8.6 Spills

o If HF is spilled outside fume hood, evacuate the area, close the doors, post the area with a sign to
prevent others from entering, and call 5000.
o Small  spills  inside  the  fume  hood  can  be  cleaned  up  by  a  laboratory  staff  if  they  have  received  
spill  cleanup  training,  have  the  correct  equipment,  understand  the  hazards,  and  are  confident  in  
their  ability  to  clean  up  the  spill  safely  and  dispose  of  waste  properly.  Lime  soda,  ash,  sodium  
bicarbonate,  or  a  spill  absorbent  specified  for  HF  should  be  used  for  cleanup.  Organic  spill  kits  
that  contain  Floor-­‐Dri,  kitty  litter,  or  sand  should  not  be  used  because  HF  reacts  with  silica  to  
produce  silicon  tetrafluoride,  a  toxic  gas.    

2.8.7 Hazards Associated with Other Fluorine Compounds

o Many  chemicals  containing  fluorine,  such  as  ammonium  fluoride,  sodium  fluoride,  sulfur  
tetrafluoride,  and  ammonium  bifluoride,  may  react  with  acid  or  water  to  produce  HF.  Review  
the  MSDS  of  all  fluoride  compounds  carefully  for  safety  precautions  to  reduce  the  risk  of  
creating  a  HF  hazard.  If  the  manner  in  which  the  fluorine  compound  is  used  can  create  HF,  
follow  the  precautions  for  HF  and  keep  topical  antidote  on  hand.  
 HYDROFLUORIC ACID FIRST AID INSTRUCTIONS
POST THESE FIRST AID INSTRUCTIONS IN THE ROOM WHERE THE
HYDROFLUORIC ACID IS USED OR HANDLED.

Location of calcium gluconate gel:

Building and Room:
Exact Location in Room:
BACKGROUND ON HF

Hydrofluoric acid (HF) exposure is very toxic and can be fatal if not treated
immediately. HF is absorbed quickly; however, damage/symptoms can occur hours to
days later. Any person exposed to HF must have immediate first aid, followed
by immediate medical treatment from a physician. When seeking medical attention
bring a copy of the HF Material Safety Data Sheet to the Emergency Room.

SKIN EXPOSURE EYE

1. Immediately flush affected area with water E
1. Immediately flush eyes with water for at least
XPOSURE
for 15 minutes under emergency 15 minutes under emergency eyewash or
eyewash/shower station or other water other water source. If only one eye is
source. Remove all contaminated clothing affected, be careful not to flush
while flushing with water. contaminated water into the other eye.
2. After flushing, apply calcium gluconate to If possible, provide continuous
burn site with clean, gloved hand. Continue irrigation during transport.
massaging gel into the affected area of skin. 2. Do not apply calcium gluconate gel to eyes.
Reapply every 15 minutes until emergency 3. For emergency medical assistance, call:
medical assistance arrives. • 5000
3. For emergency medical assistance, call:
• 5000

INHALATION INGESTIO
1. Remove to fresh air 1. nNNnnN
Do not induce vomiting.
2. Get medical assistance immediately, call: 2. Rinse mouth with cold water.
• 5000 3. If the victim is conscious, have them drink
lots of water to dilute the acid.
3. For emergency medical assistance, call:
• 5000
Table : Compressed Gas Association Connection Chart for Regulators
[from CCE Safety Manual]
CYLINDER GAS TYPE CHEMICAL SYMBOL CGA CONNECTION
Standard/Alternate
Acetylene C 2H 2 510/300
Air ---- 590/346
Allene CH2:C:CH2 510
Ammonia Anhydrous NH3 240/705
Ammonia (VHP) --- 660
Antimony Penta Fluoride SbF5 330
Argon Ar 580
Argon (Research Grade) --- 590
Arsine AsH3 350/660
Boron Trichloride BCl3 660/330
Boro Trifluoride BF3 330
Bromine Pentafluoride BrF5 670
Bromine Trifluoride BrF3 670
Bromoacetone BrCH2COCH3 300/660
Bromochlorodifluoromethane CBrClF2 668/660
Bromochloromethane CH2BrCL 668/660
Bromotrifluoroethylene Br FC:CF2 510/660
Bromotrifluoromethane CBrF3 668/320, 660
1,3 - Butadiene CH2:CHCH:CH2 510
Butane CH3CH2CH2CH3 510
Butenes CH3CH2CH:CH2 510
Carbon Dioxide CO2 320
Carbon Monoxide CO 350
Carbonyl Fluoride COF2 660/750
Carbonyl Sulfide COS 330
Chlorine CL2 660
Chlorine Pentafluoride CLF5 670
Chlorine Trifluoride ClF3 670
Chlorodifluoroethane CH3CCL F2 510/660
Chlorodifluoromethane CH Cl F2 660/668
Chlorofluoromethane CH2 Cl F 510
Chloroheptafluorocyclobutane C4F7Cl 660/668
Chloropentafluoroethane C2CLF5 668/660
Chlorotrifluoromethane CClF3 668/320,660
Cyanogen C 2N 2 750/660
Cyanogon Chloride CNCl 750/660
Cyclobutane C 4H 8 510
Cyclopropane C 3H 6 510
Deuterium D2 350
Deuterium Chloride DCl 330
Deuterium Fluoride DF 330
Deuterium Selenide D2 Se 350 / 330
Deuterium SulFide D 2S 330
Diborane B 2H 6 350
Dibromodifluoroethane C2H2Br2F2 668/660
Dibromodifluoromethane CBr2F2 668/660
1,1 - Difluoroethylene FCH:CHF 320
Dichlorosilane H2 Si Cl2 330/510
Diethylzinc (C2H5)2Zn 750
Dimethylamine (CH3)2NH 705/240
Dimethyl Ether CH3OCH3 510
2,2 Dimethyl Propane C(CH3)4 510
Diphosgene ClCO2CCl3 750/660
 Ethane C 2H 6 350
Ethane (Research Grade ) --- 350
Ethylacetylene CH3CH2:CH 510
Ethylchloride CH3CH2Cl 510/300
Ethyldichloroarsine C2H5AsCl2 750/660
Ethylene CH2:CH2 350
Ethylene Oxide C 2H 4O 510
Ethyl Ether (C2H5)2O 510
Ethyl Fluoride C 2H 5F 750/660
Fluorine F2 679/670
“Freon 12 “ (Dicholordifluoromethane) Cl2 660
“Freon 13 “ (Chlorotrifluoromethane) CClF3 320
“Freon 1381” (Bromotrifluoromethane) CBrF3 320
“Freon 14 “ (Tetrafluoromethane) CF4 320
“Freon 22” (Chlorodifluoromethane) CHClF2 660/620
“Freon 114” (1,2 –
Dichlorotetrafluoroethane)
Cl F2 CCCl F2 660
“Freon 116 “ (Hexafluoroethane) C 2F 6 320
“Freon 8318” (Octafluorocyclobutane) C 4F 8 660
“Genetron 21” (Dichlorofluoromethane) CHCl2F 660
“Genetron 23” (Fluoroform) CH F3 320
“Genetron115”
(Monochloropentafluoroethane)
Br F2 CCF3 660
“Genetron 152A “ (1,1 – Difluoroethane) F CH2 CH2 F 660
Germane Ge H4 660/750
Helium He 580/677
Heptafluorobutyronitrile C 4F 7N 750/660
Hexafluoracetone C 3F 6O 660/330
Hexafluorocyclobutene C 4F 6 750/660
Hexafluorodimethyl Peroxide CF3OOCF3 755/660
Hexafluoroethane C 2F 6 660/668
Hexafluoropropylene CF3CF:CF2 668/660
Hydrogen H2 350
Hydrogen Bromide HBr 330
Hydrogen Chloride HCL 330
Hydrogen Cyanide HCN 750/160
Hydrogen Fluoride HF 330/660
Hydrogen Iodide HI 330/660
Hydrogen Selenide H2Se 350/660
Hydrogen Sulfide H 2S 330
Iodine Pentafluoride IF5 670
Isobutane C4H10 510
Isobutylene C 4H 8 510
Krypton (research Grade) Kr 590
“Manufactured Gas B” --- 350
“Manufactured Gas C” --- 350
Lewsite ClCH:CHAsCL2 750/660
Methane CH4 350
Methylacetylene CH3C:CH 510
Methyl Bromide CH BR 320/660
3-Methyl – 1 -butene (CH3)2CHCH:CH2 510
Methyl Chloride CH3Cl 660/510
Methyldichloroarsine CH3AsCl2 750
Methylene Fluoride CH2F2 320
Methyl Ethyl Ether CH3OC2H5 510
Methyl Fluoride CH3F 350
Methyl Formate HCOOCH3 510/660
Methyl Mercaptan CH3SH 330/750
Monoethylamine CH3CH2NH2 240/705
Monomethylamine CH3NH2 240/705
Mustard Gas S(C2H4Cl)2 750/350
Natural Gas --- 350/677
Neon Ne 590/580
Nickel Carbonyl Ni (CO)4 320/750
Nitric Oxide NO 660/755, 160
Nitrogen N2 580
Nitrogen (Research Grade) --- 590
Nitrogen Dioxide NO2 660/160
Nitorgen Trifluoride NF3 679
Nitrogen Trioxide N 2O 3 660/160
Nitrosyl Chloride NOCl 660/330
Nitrosyl Fluoride NOF 330
Nitrous Oxide N 2O 326
Nitryl Fluoride NO2F 330
Octafluorocyclobutane C 4F 8 660/668
Octafluoropropane C 3F 8 660/668
Oxygen O2 540
Oxygen Difluoride OF2 679
Ozone O3 660/755
Pentaborane B 5H 9 660/750
Pentachlorofluoroethane CCl3CCl2F 668/660
Pentafluoroethyl Iodine CF3CF2I 668/660
Pentafluoropropionitrile CF3CF2CN 750/660
Perchloryl Fluoride ClO3F 670
Perfluorobutane C4F10 668
Perfluorobutene – 2 C 4F 8 660
Phenylcarbylamine Chloride C6H5N : CCl2 330/660
Phosgene COCl2 660
Phosphine PH3 660/350
Perfluoropropane --- 660
Phosphorous Pentafluoride PF5 330
Phosphorous Trifluoride PF3 330
Propane C 3H 8 510
Propylene C 3H 6 510
Silane SiH4 350/510
Silicon Tetrafluoride SiF4 330
Stibine SbH3 350
Sulfur Dioxide SO2 660/668
Sulfur Hexafluoride SF6 590/668
Sulfur Tetrafluoride SF4 330
Sulfuryl Fluoride SO2F2 660/330
1, 1, 1, 2 – Tetrachlorodifluoroethane C2Fl4F2 668/660
1, 2, 2, 2, - Tetrafluorochloroethane – 1 C2HClF4 668/660
Tetrafluoroethylene C 2F 4 350/660
Tetrafluorohydrazine N 2F 4 679
Tetrafluoromethane CF4 580/320
Tetramethyllead (CH3)4Pb 750/350
Trichlorofluoromethane CCl3F 668/660
Trichlorotrifluoroethane CF3CCl3 668/660
Triethylaluminum (C2H5)3Al 750/350
Triethylborane (CH5)3B 750/350
Trifluoroacetonitrile CF3CN 750/350
Trifluoroacetyl Chloride CF3COCl 330
1, 1, 1 – Trifluoroethane CH3CF3 510
Trifluoroethylene C 2F 3H 510
Trifluoromethyl Hypofluorite CF3OF 679
Trifluoromethyl Iodide CF3I 668/660
Trimethylamine (CH3)3N 240/705
Trimethylstibine (CH3)3Sb 750/350
Tungsten Hexafluoride WF6 330/679
Uranium Hexafluoride UF6 330
Vinyl Bromide C2H3Br 320/510
Vinyl Chloride C2H3Cl 290/510
Vinyl Fluoride C 2H 3F 320/350
Vinyl Methyl Ether C2H3OCH3 290/510
Xenon Xe 580/677
Xenon (Research Grade) --- 590