Outstanding in every position

The complete range of monolithic power with Chromolith HPLC columns

How can I enhance efficiency of high performance separations and reduce costs?

Quite simply: By using EMD Chemicals Chromolith HPLC technology, which gives high performance at low pressure and longer column lifetime, now for even more application areas.
That's what's in it for you. EMD Chemicals

Your benefits
Improved HPLC system security Column length no longer pressure limited Speed of analysis Standard HPLC instruments are ideally suited for use with Chromolith HPLC columns Cost savings

Our team for your success

Technology overview Chromolith CapRod capillary columns Chromolith analytical columns (2 mm i.d.) Chromolith analytical columns (3 mm i.d.) Chromolith analytical columns (4.6 mm i.d.) Chromolith semi-prep columns (10 mm i.d.) Chromolith prep columns (25 mm i.d.) Ordering information
NEW

4-5 6 -7 8-9 10 -11 12 -15 16 -17 18 19

NEW

Chromolith HPLC columns a revolutionary technology wins worldwide acclaim

Scientists throughout the world have rapidly realized the revolutionary benefits made possible by the Chromolith HPLC column technology. Hundreds of scientific publications have been published.

Revolutionary monolithic silica replaces particles

Chromolith HPLC columns are not filled with minute silica particles like conventional packed HPLC columns, but consist of a single rod of high purity monolithic silica.
Mesopores: 13 nm Macropores: 2 m

Revolutionary bimodal pore structure

Chromolith silica has a porosity exceeding 80 % and a unique bimodal pore structure, which gives greatly improved chromatographic performance in terms of separation performance and column back-pressure. Macropores dramatically reduce the column back-pressure and allow the use of faster flow rates, thereby considerably reducing the analysis time. Mesopores form the fine porous structure and provide the very large active surface area for high efficiency separations.
SEM picture of a cross section from a silica monolith Total porosity > 80 %

Revolutionary good peak resolution, low column back-pressure

When compared with 5 m particulate columns of the same length, the column back-pressure with Chromolith HPLC columns is typically 4 times lower. The peak resolution however remains virtually unchanged.

Revolutionary the long lifetime and robustness

Monolithic Chromolith HPLC columns demonstrate very high mechanical stability and long operative lifetimes, in most cases far exceeding column lifetimes for particulate columns.
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All benefits at a glance

Speed of analysis
Separations two times faster at half the column back-pressure compared to 5 m columns Higher sample throughput separations up to 9 times faster if required Fast column re-equilibration between analyses

Improved HPLC system security

Significantly increased column lifetime Reduced maintenance on HPLC pump and injector seals Reduced need for sample preparation as columns are very resistant to blocking (even with biological samples)

Column length no longer pressure limited

Very high peak resolution by column coupling

Easy to use
Chromolith columns cladded in PEEK are very easy-to-use and handle

Cost savings
Cost savings due to faster sample throughput (in USA typically $ 400 per day if analysis time is halved) can repay revalidation expenses (in USA typically $ 12,000) in about 6 weeks Cost saving due to significantly longer column lifetime

The speed and performance of particulate HPLC columns are limited by column pressure
Particulate HPLC columns are filled with particles, typically silica with 3 or 5 micron particle size. Very small particle size brings one advantage high separation performance. But small particle size also brings three limitations: high column back-pressure limits the speed of analysis and the column length particulate columns can easily block, thereby reducing lifetime lifetime of HPLC instrument and injector seals is reduced at high pressure

Acquity UPLC columns use even smaller particles (1.7 m) requiring ultra-high pressures. Here the user requires a special new instrument, in order to operate at these high pressures.
(Acquity UPLC is a trademark of Waters Corporation)

Chromolith CapRod capillary columns

Fast nano-LC for proteins and peptides
Chromolith CapRod capillaries combine the speed of monolithic silica technology with the sensitivity of nano-LC, thus enabling high speed high sensitivity LC / MS analytics.

NEW

Monolithic versus particulate capillary

100 Column: Chromolith CapRod RP -18 endcapped, 150 mm x 100 m Flow rate: 1 l / min 50 50 100 Column: Particulate 75m RP-18 capillary Flow rate: 0.2 l / min

10

12

14 Time (min)

10

15

20

25

30

35

40 Time (min)

Separation of Trypsin digested BSA (200 fmol) on monolithic and particulate capillaries using gradient conditions of water and acetonitrile with 0.08% - 0.1% formic acid.

Separation of proteins
mAU 30 25 20 15 REM of column cross-section showing monolithic structure 10 5 0 CapRod column

10

15

20 25 27 Time (min)

A mixture of four proteins (12 kDa - 85 kDa: Cytochrome C, Ribonuclease A, BSA, Apo-Transferrin) was separated on a Chromolith CapRod 100m RP-8e capillary under gradient conditions using water and acetonitrile with 0.08 % - 0.1 % formic acid as the mobile phase.

Reproducibility of peptide separations

mAU 14 12 10 6 6 4 2 0

0 2.5 5 7.5 10 12.5 15 17.5 20 Time (min)

Various chromatograms of up to 100 injections are shown. 500 fmol Cytochrome C was digested with Trypsin and subsequently separated on a Chromolith CapRod 50 m RP-18e capillary at a flow rate of 500 nl / min using a gradient with 2 % - 40 % water/Acetonitrile (20 % / 80%, v:v) in 0.08 % formic acid ramped for 10 minutes. There is no obvious difference in the separation between the first run (bottom) and after 100 injections (top).

A versatile set of capillaries to choose from

Recommended use Separation of small molecules Separation of peptides Separation of proteins Micro ESI Nano ESI High Resolution Flow Rates ( l/min ) 0.1 - 0.8 0.4 - 3 0.4 - 3 RP-18e RP-8e 150 x 0.05 mm 150 x 0.1mm RP-18e RP-18e 150 x 0.1mm 150 x 0.1mm HR 0.1 - 0.5 3 - 12 0.5 - 2 >1 RP-18e RP-18e 150 x 0.2 mm 150 x 0.2 mm HR RP-18e 50 x 0.2 mm Trap

Features of CapRod capillaries:

High flow rates at low pressure Long lifetime of the column Robust handling

Chromolith FastGradient RP-18 endcapped 50 - 2 mm

The new top-player in monolithic HPLC columns for UHPLC, UPLC or standard HPLC
Ultra-high performance in combination with extraordinary low operating pressure makes the Chromolith 2 mm column technology unique. Excellent ultra-fast results are obtained, not only in the new UHPLC and UPLC instruments, but equally well in all standard HPLC systems with low dead volume.

NEW

Ultra-fast separation of Antihistamines

6 2 3 10 5 t0 4 7 1 9 8 Column Mobile phase Gradient Flow rate Pressure Detection Temp. Inj. Volume Sample Chromolith FastGradient RP-18e 50 - 2 mm A: 0.1% TFA in water B: 0.1% TFA in ACN 5 % to 90 % B in 3.4 min 1.0 mL / min 50 -120 bar UV 230 nm ambient 0.2 L 1) Phenylephrine 6) Chloropyramine 2) Tripelenamine 7) Diphenhydramine 3) Pyrilamine 8) Promethazine 4) Chloropheniramine 9) Loratadine 5) Brompheniramine 10) Meclizine

0 Figure 1

1.25

2.5 Time (min)

In Figure 1 the separation of ten antihistamine pharmaceutical standards is complete in less than 2.5 minutes and the column back-pressure at all times during the gradient is less than 120 bar (1740 psi ). In order to achieve the same separation on a 2 mm internal diameter sub-2m particle column with a UHPLC system, an operating pressure close to 1000 bar (14,500 psi ) is required.

Your benefits:
Flexibility this column gives very fast, high performance results with all low dead volume LC instruments, whether UHPLC, UPLC or standard HPLC Flow rates from 0.2 - L in give ideal compatability with LC S systems, 1m /m /M both with ESI and APCI interfaces Long column lifetime and resistance to column blocking (thanks to monolithic silica structure and absence of frits) give method robustness and cost saving
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Chromolith FastGradient RP-18 endcapped 50 - 2 mm recommended particularly for very fast gradient applications in pharma R&D, chemicals, food and academic labs.
Ultra-fast separation of anabolic steroids for LC / MS
In Figure 2 the separation of eleven anabolic steroid standards, as might be required in a doping test, is complete in less than 2.5 minutes and the column back-pressure during the gradient analysis was always less than 135 bar (1960psi). As in Figure 1, similar ultra-fast analyses can be achieved on specialized UPLC systems at pressures around 1000 bar.

Anabolic steroids on Chromolith FastGradient RP-18e 50-2mm Mobile Phase: Gradient: A: Acetonitrile (LiChrosolv) B: Water (LiChrosolv) time A B flow [min] [%] [%] [ml/min] 0.0 15 85 1.50 0.4 15 85 1.50 2.0 100 0 1.00 2.5 100 0 1.00 2.6 15 85 1.00 3.0 15 85 1.00 0.5L 240 nm UV 40C 1.) Fluoxymesterone 2.) Boldenone 3.) Methandrostenolone 4.) Testosterone 5.) Methyltestosterone 6.) Boldenone acetate 7.) Testosterone acetate 8.) Nandrolone propionate 9.) Testosterone propionate 10.) Nandrolone phenylpropionate 11.) Testosterone isocaproate

4 6 2 3 5

10 8 9 11 12

Injection: Detection: Temperature: Sample:

1 0 Figure 2 0.5 1.0 1.5 2.0 2.5 Time (min)

Ultra-fast results at low back-pressure for isochratic applications

mAU 500 400 300 200 1 100 0 0.0 Figure 3 1.0 2.0 3.0 4.0 Time (min) 2 3 4 Isocratic 5 Column Mobile phase Flow rate Pressure Detection Temp. Inj. Volume Sample Chromolith FastGradient RP-18e 50 - 2 mm 60 /40 Acetonitrile /water 0.4 mL / min 30 bar UV 254 nm 25 C 1 L 1) Thiourea 2) Biphenyl-2-ol 3) Progesterone 4) Hexanophenone 5) Anthracene

Chromolith Performance RP -18 endcapped 100 - 3 mm

Extends the team of Chromolith HPLC columns
Based on the same high purity silica
Chromolith 3 mm HPLC columns are based on the same robust high purity monolithic silica with 2 m macropores and 130 mesopores as Chromolith 4.6 mm and Chromolith 10 mm i.d. columns. Chromolith 3 mm operates at lower flow rates than Chromolith 4.6 mm and methods are easily transferred.

Fast separations at lower flow rates

Figure 1 shows a typical fast separation of four compounds in less than two minutes using a Chromolith 4.6 mm internal diameter column at 4 mL /min. Figure 2 shows the same separation on Chromolith 3 mm i.d. column at just 1.7 mL /min. Both chromatograms show excellent column efficiency and peak resolution.

Figure 1 Chromolith RP -18e 100 - 4.6 mm 1 Flow rate: 4.0 mL /min Back pressure: 137 bar

Figure 2 Chromolith RP -18e 100 - 3 mm 1 Flow rate: 1.7 mL /min Back pressure: 100 bar 3 Mobile phase: Detection: Temperature: Inj. Volume: Sample: Acetonitrile / water 40 /60 UV 254 nm 2.4 L flow cell * ambient 1 L * 1) Biphenyl-4.4 -ol 2) Biphenyl-2.2 -ol 3) Biphenyl-4-ol 4) Biphenyl-2-ol

3 2 4 2

0.0

1.0

2.0 Retention time (min)

0.0

1.0

2.0 Retention time (min)

* For optimum results with 3 mm columns, extra-column volume must be small.

Chromolith Performance RP -18 endcapped 100 - 3 mm

Your benefits:
Ideal for reducing the time of analysis at low flow rates Ideal for use with standard HPLC instruments Operates at lower flow rates than 4.6 mm i.d. columns cost saving for solvents (typically > $ 1000 per annum) Increased detection sensitivity
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Fast high resolution analysis with one column

Chromolith Performance RP -18 endcapped 100 - 3 mm is an ideal alternative to conventional particulate columns with internal diameter 4.6, 4 or 3 mm. Even difficult separations, which often take 15 - 30 minutes on particulate columns, typically take only 5 - 10 minutes on Chromolith 3 mm. Figure 3 shows an excellent separation of 9 pharmaceutical compounds in just 4 minutes

Figure 3 Chromolith RP -18e 100 - 3 mm Flow rate: 2.0 mL /min Back pressure: 92 bar 8

Figure 4 2 coupled Chromolith RP -18e 100 - 3 mm Flow rate: 1.5 mL /min Back pressure: 140 bar 8 2 Mobile phase: Detection: Temperature: Inj. Volume: Sample: 9 Acetonitrile / buffer pH 1.8 (gradient) UV 254 nm 30 C 1 L 1 - 2) by-products 3) Levothyroxine 4 -9) by-products

2 4 3 5 1 0.0 2.5 5.0 Retention time (min) 0.0 1 6 7 9 3

6 5

4.0

8.0 Retention time (min)

Two columns coupled together give extra high resolution

Chromolith 3 mm columns are easily coupled using the column coupler (1.51467.0001) to give columns 20 cm or longer as required. The result is shown in Figure 4 very high peak resolution at moderate pressure with flow rates between 1-1.5 mL /min.

Two columns coupled together

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Chromolith Performance 100 - 4.6 mm columns

For faster separations at lower back-pressure
Chromolith Performance is the ideal alternative to a 5 m particulate column
At 1 mL/min flow rate a chromatogram run on a Chromolith Performance column looks almost identical to the same chromatogram run on the corresponding particulate column. The striking difference is that the column back-pressure is typically 4 times lower compared to a 5 m column.

Three times faster analysis at lower column pressure

Chromolith Performance 100 - 4.6 mm

Simply by increasing the flow rate to 3 mL/min, the analysis is 3 times faster, the quality of the separation is unchanged and the column back-pressure remains lower than with the 5 m column. If even faster analysis is required, simply increase the flow rate further up to 9 times faster.

2 1 3 4 5

Beta Blockers 1 mL/min and 3 mL/min Column: Mobile phase: Detection: Inj. Volume: Sample: Chromolith Performance RP -18e 100 - 4.6 mm Acetonitrile/0.1 % TFA in water (20/80 v/v) UV 220 nm 5 L 1) Atenolol 2) Pindolol 3) Metoprolol 4) Celiprolol 5) Bisoprolol

6 12 1 mL/min17 bar Time (min)

6 12 3 mL/min 51 bar Time (min)

Easy method transfer from particulate to Chromolith HPLC columns

Method transfer from particulate to monolithic Chromolith columns is made easy as the chromatographic selectivity of Chromolith columns is very similar to that of many modern particulate HPLC columns (e.g. Purospher). Chromolith columns are now included in the US Pharmacopoeia in the L1, L3 and L7 categories.

Choice of selectivity
RP -18 endcapped and RP -8 endcapped for reversed-phase HPLC Unmodified silica for normal phase HPLC

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Chromolith Validation Kit For correct method validation, it is essential to assess all possible sources of variations. To assist the validation process, the Chromolith Validation Kit includes three columns from three different production batches.

Chromolith Column Coupler

Very high efficiency separations at moderate pressure
Column Coupler

Longer columns give greatly improved peak resolution

Using the Chromolith column coupler, longer columns are readily available, giving high separation performance at a fraction of the back-pressure that 5 m particles generate.

Connecting ten 100 mm columns can give column efficiency exceeding 80,000 theoretical plates. Connecting four 100 mm columns will double the peak resolution.
0

5 3 2 4

81,000 plates at 85 bar pressure Column: Mobile phase: Flow rate: Detection: Temp.: Inj. Volume: Sample: 10 columns of Chromolith Performance RP -18e, 100 4.6 mm 80 /20 Acetonitrile/water 1 mL/min UV 254 nm ambient 10 L 1) Thiourea 5) Propylbenzene 2) Benzene 6) Butylbenzene 3) Toluene 7) Penylbenzene 4) Ethylbenzene

20

40 Time (min)

Column length Back pressure Plate Number N (mm) (bar) (anthrancene) 200 300 400 500 22 33 44 54 19,000 27,000 35,000 41,000

Chromolith column length and plate count at 3 mL/min.

Excellent batch-to-batch reproducibility

The batch-to-batch reproducibility of Chromolith HPLC columns is tightly controlled and fulfils the requirements for QA/QC laboratories.
4 6 1 3 2 5 7 8 9 Batch 1 Batch 2 Batch 3 Batch 4 Batch 5 0 7 14 Time (min)

Test of 5 batches

Chromatographic Conditions Column: Mobile phase: Flow rate: Detection: Temp.: Sample: Chromolith Performance RP -18e, 100 4.6 mm Methanol/water 55 /45 (v /v) 1 mL/min UV 254 nm ambient 1) Thiourea 5) 4-Ethylaniline 2) Aniline 6) Diethylphthalate 3) Phenol 7) N,N-Dimethylanilin 4) 2,3-Dihydroxy- 8) Toluene naphthalene 9) Ethylbenzene

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Chromolith SpeedROD 50 4.6 mm Chromolith Flash 25 4.6 mm

Ultra-fast separations Ideal for DMPK and fast LC / MS
Chromolith SpeedROD and Flash are the ideal alternatives to short high performance 3 m particulate HPLC columns
The plate count can exceed 100,000 plates/meter, yet the column back-pressure is typically 8 times less than a 3 m column! Many publications demonstrate the use of these columns in very fast gradient applications, where Chromolith columns typically have much longer lifetimes than particulate columns.

Separation of food additives

Column: Mobile phase: 1 2 5 6 Gradient: Chromolith SpeedROD RP -18e, 50 - 4.6 mm A: Acetonitrile B: 0.01 M Phosphate buffer pH 5.0 Time/min %A %B 0.0 3 97 2.5 3 97 2.6 8 92 5 8 92 4 mL/min 48 - 50 bar 227 nm ambient 10 L 1) Acesulfame - K 23 g/mL 2) Saccarine 29 g/mL 3) Benzoic acid 13 g/mL 4) Sorbic acid 1 g/mL 5) Caffeine 47 g/mL 6) Aspartame 100 g/mL

4 3

Flow rate: Pressure: Detection: Temp.: Inj. Volume: Sample: 2.3 4.6 Time (min)

Sometimes the time per analysis can be under one minute!

The benefits of obtaining accurate quantitative results fast cannot be underestimated and can lead to major cost savings. The chromatogram here shows the rapid analysis of tocopherols in biological matrix (chromatogram courtesy of AS Vitas, Norway; www.vitas.no).

Tocol 0 0.1

Column: Mobile phase: Detection:

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Chromolith SpeedRod RP -18 endcapped Water: Methanol (2 : 98, v/ v) Fluorescence detection Ex: 295, Em: 330 Injection volume: 1 L Column temp: 40 C Sample: Plasma precipitated with 3 volumes of 2-propanol

0.2

Alpha.tocopherol 0.3 Time (min)

Chromolith SpeedROD at 7 mL in /m

Rugged, reliable and long-lasting

A characteristic of all Chromolith columns
Long column lifetime and high resistance to column blockage reduce costs per analysis and enhance data integrity
Chromolith HPLC columns have demonstrated immense robustness and set a new standard for long column lifetime. The rigid monolithic silica skeleton with 2 m macropores is the reason for this improved performance. The diagrams show the results of a stability test with 3,300 injections and 50,000 column volumes of mobile phase.

Retention time [min] 4 3,5 3 2,5 2 1,5 1 0,5 0 0 500 1000 1500 2000 Progesteron Anthracene 2500 3000 Injection No.

Intensity (mV)

Chromatographic conditions Column: Mobile phase: Detection: Sample: Chromolith Performance RP -18e, 100 - 4.6 mm Acetonitrile/water 60/40 (v/V) UV 254 nm Thiourea 10 g/mL Progesteron 100 g/mL Anthracene 10 g/mL

0.68 0.68 0.68

1.65

3.56 3.61 3.59

2.09

The three superimposed chromatograms show the reproducibility of the first, the 1500 th and the 3000 th injections.

2.12 2.11

3300 injections 1500 injections 1. injection

0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.6 Retention Time (min)

Literature references demonstrating Chromolith HPLC column stability include: 1) V. Borges et al. J. Chromatography B, 804 (2004) 277-287, 2) J-T Wu et al. Rapid Commun. Mass Spectrom. 15 (2001) 1113-1119

Chromolith Guard Columns Chromolith Guard Columns further extend the column lifetime and further protect the column from both particulate and chemical contamination. Guard Columns are available in 5 mm or 10 mm lengths and in kits complete with Guard Column holder.
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1.65 1.66

Perfect scale-up from analytical to preparative LC

Chromolith SemiPrep 100 -10 mm RP -18 endcapped
Optimum separation at flow rates exceeding 40 mL min /
Chromolith SemiPrep 10 mm i.d. columns combine high separation speed with very high separation performance. They are the ideal alternative to particulate columns with 10 mm i.d. (and even 21.2 mm).

Separation of a standard mixture

Acetonitrile/water 60 /40 , Data for anthracene (3rd peak)

mAU 11 10 9 8 7 6 5 4 3 2 1 0 1 2

mAU approx. 90,000 N/m 7 mL/min 8 7 6 5 4 3 2 1 3 4 5 Time (min) 0 0 1 2 3 Time (min) 19 mL/min

mAU 7 6 5 4 3 2 1 0 0

43 mL/min 100 bar

1 2 Time (min)

Accurate scale-up from analytical to preparative columns

25 mg injected onto a Chromolith SemiPrep RP -18 endcapped column show the same excellent separation when compared with the corresponding analytical column.
mAU 39 34 29 24 19 14 9 4 0 1 3 2 1 mAU 1000 800 600 400 200 4 2 3 4 5 6 7 8 9 10 Time (min) 0 1 2 3 4 5 6 7 8 9 10 Time (min) Detection: 1 2 3 Chromatographic conditions Column: Gradient: Chromolith RP -18e A: Acetonitrile with 0.1 % TFA, B: Water with 0.1 % TFA, 0 10 min 15 80 % A UV 270 nm

Performance 100 - 4.6 mm Flow rate: Injection: Sample: 1 mL/min 2 L 1) Nadolol 1 mg/mL 2) Metoprolol 1 mg/mL 3) Propranolo 0.5 mg/mL

SemiPrep 100 -10 mm Flow rate: Injection: Sample: 4.7 mL/min 100 L 1) Nadolol 100 mg/mL 2) Metoprolol 100 mg/mL 3) Propranolol 50 mg/mL

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Chromolith SemiPrep 100 10 mm

For high throughput purification in drug discovery

Sample Loadability

mAU 2000 1500 1000 500 0 0 1 2 By courtesy of Dr. A. Espada and C. Anta, Lilly Spain 3 4 1 DMSO 2

Separation of 80 mg/injection

6 Time (min)

Chromatographic conditions Column: Mobile phase: Gradient: Chromolith SemiPrep RP -18e, 100 -10 mm A: Acetonitrile with 0,05 % TFA B: Water with 0,05 % TFA 0 - 1 min 5 % A; 1 - 5 min 5 - 90 % A; 5 - 5.2 min 95 % A; 5.2 - 6.2 min 95 % A Flow rate: Detection: Injection volume: Sample: 8 mL /min UV 214 nm 400 L 1) Propranolol 2) Nifedipine

200 mg/mL 200 mg/mL dissolved in DMSO/Methanol 1/1

The sample loadability depends on many factors including the solubility of the sample in the mobile phase. The following example shows that the sample loadability on the Chromolith SemiPrep column can exceed 80 mg. Here DMSO/Methanol (1:1) is used as solvent.

LC/MS Compatibility
Chromolith SemiPrep columns are optimized for LC/MS by a surface modification process minimizing column bleed.

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Chromolith prep 100 - 25 mm for HighSpeed Prep Chromatography

Highest Productivity Chromolith prep 25 mm i.d. column
Chromolith 25 mm i.d. increases the flow rate enormously
The aim of a chromatographic separation at production scale is to optimize sample throughput as a function of time. The Chromolith 25 mm i.d. prep column increases the flow rate enormously, compared to particulate preparative columns, and enables optimum process productivity at production scale. More information? Ask for the Chromolith prep brochure, which may also be downloaded from www.merck-chemicals.com

Specifications of Chromolith HPLC Columns

Silica type: Particle size: Macropore size: Mesopore size: Pore volume: Surface area: Total porosity: Pharmacopoeia classification: high purity (99.999 %) monolithic 2 m 13 nm (130 ) 1.0 mL/g 300 m2/g > 80 % L3 (USP) Mobile phase compatibility:
All standard HPLC solvents may be used with the following restrictions:

Max. dichloromethane conc.: Max. tetrahydrofuran conc.: Max. dimethylsulphoxide DMSO: pH range: Max. pressure: Max. pressure: Max. pressure: Max. temperature

5% 50 % 5 % but OK as sample solvent 2 - 7.5 200 bar for 4.6 mm columns 150 bar for 10 mm columns 100 bar for 25 mm columns 50C

Surface modification: Selectivity equivalent to: Carbon content: Surface coverage:

RP -18 endcapped L1 (USP) 18 % 3.6 mol/m2

Surface modification: Selectivity equivalent to: Carbon content:

RP -8 endcapped L7 (USP) 11 %

Chromolith prep columns have 3m macropore size, 12 nm mesopore size, and surface area is 350 m2/g

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Ordering information
Description Capillary columns Chromolith CapRod RP -18 endcapped Chromolith CapRod RP -18 endcapped Chromolith CapRod RP - 8 endcapped Chromolith CapRod RP -18 endcapped HR Chromolith CapRod RP -18 endcapped Trapping Chromolith CapRod RP -18 endcapped Chromolith CapRod RP -18 endcapped HR Analytical columns 150 mm 150 mm 150 mm 150 mm 50 mm 150 mm 150 mm 0.05 mm 0.1 mm 0.1 mm 0.1 mm 0.2 mm 0.2 mm 0.2 mm 1 column 1 column 1 column 1 column 1 column 1 column 1 column 1.50403.0001 1.50402.0001 1.50400.0001 1.50404.0001 1.50409.0001 1.50405.0001 1.50407.0001 Length Internal Items diameter Ord. No.

Chromolith FastGradient RP -18 endcapped

50 mm

2 mm

1 column

1.52007.0001

Chromolith Performance RP -18 endcapped Chromolith Flash RP -18 endcapped Chromolith SpeedROD RP -18 endcapped Chromolith Performance RP -18 endcapped Chromolith Performance RP-8 endcapped Chromolith Performance Si Chromolith Column Coupler Chromolith Validation Kit RP -18e (3 columns from different batches) Guard columns Chromolith RP -18 endcapped Guard Column Chromolith RP -18 endcapped Guard Column Chromolith RP -18 endcapped Guard Column kit Chromolith RP -18 endcapped Guard Column kit Semi-preparative and preparative columns

100 mm 25 mm 50 mm 100 mm 100 mm 100 mm 100 mm

3 mm 4.6 mm 4.6 mm 4.6 mm 4.6 mm 4.6 mm 4.6 mm

1 column 1 column 1 column 1 column 1 column 1 column 1 piece 3 columns

1.52001.0001 1.51463.0001 1.51450.0001 1.02129.0001 1.51468.0001 1.51465.0001 1.51467.0001 1.51466.0001

5 mm 10 mm 5 mm 10 mm

4.6 mm 4.6 mm 4.6 mm 4.6 mm

3 pieces 3 pieces 3 pieces + holder + tool 3 pieces + holder + tool

1.51451.0001 1.51452.0001 1.51470.0001 1.51471.0001

Chromolith SemiPrep RP -18 endcapped Chromolith prep Si Chromolith prep RP -18 endcapped

100 mm 100 mm 100 mm

10 mm 25 mm 25 mm

1 piece 1 piece 1 piece

1.52016.0001 1.25251.0001 1.25252.0001

These products are not intended for use as in-vitro diagnostics in terms of European Directive 98 /79 / EC. They are for research purposes only, for investigating in-vitro samples without any medical objective.

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Products are warranted to meet the specifications set forth on their label/packaging and/or certificate of analysis at the time of shipment or for the expressly stated duration. EMD MAKES NO OTHER WARRANTY OF ANY KIND WITH REGARD TO ITS PRODUCTS, WHETHER EXPRESS, IMPLIED, BY OPERATION OF LAW, BY COURSE OF DEALING, USAGE OF TRADE OR OTHERWISE, INCLUDING, WITHOUT LIMITATION, ANY IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. EMD SHALL NOT IN ANY EVENT BE LIABLE FOR INCIDENTAL, CONSEQUENTIAL, INDIRECT, EXEMPLARY OR SPECIAL DAMAGES OF ANY KIND RESULTING FROM ANY USE OR FAILURE OF THE PRODUCTS. EMD makes no warranties of any kind for any technical advice provided by EMD, or for any results occurring as a result of the application of such advice. All sales are subject to EMDs complete Terms and Conditions of Sale. Prices are subject to change without notice. EMD reserves the right to discontinue products without prior notice. Purospher, LiChrosorb, LiChrospher, LiChroCART, manu-CART and Hibar are registered trademarks of Merck KGaA, Darmstadt, Germany. UPLC is a trademark of Waters Corp.

EMD Chemicals Inc. 480 South Democrat Road Gibbstown, NJ 08027 (USA) Phone 800-222-0342 Fax 856-423-4389 [email protected] www.emdchemicals.com LT 1003473 1/10