Arellano University

Legarda, Manila
COLLEGE OF PHARMACY

PHARMACEUTICAL ANALYSIS II 2nd SEMESTER, A.Y. 2020 – 2021

Name of Student: Section:

MULTIPLE CHOICE QUESTIONS: Each numbered item is followed by options. Select the best answer
to each question. Some options may be partially correct, but there is only ONE BEST answer.

I. PHARMACEUTICAL ANALYSIS II 9. Principally concerned with rotational spectral

and crystal lattice vibrations
1. It refers to the distance between peaks or (A) FIR
troughs (B) MIR
(A) Wavelength (C) NIR
(B) Frequency (D) NUV
(C) Wavenumber
(D) Transmittance 10. A law stating that the power of a transmitted
radiant beam decreases exponentially as the
2. Acetylene and aldehyde groups present in a thickness of the solution containing the
compound that absorbs radiant energy are absorbing chemical species increase
called arithmetically
(A) Chromophore (A) Beer's law
(B) Inert (B) Bouguer's law
(C) Inactive (C) Lambert's law
(D) Chromosomes (D) Beer-Lambert's law

3. Theory states that radiant energy occurs in 11. Compute for the absorbance of Paracetamol
bundles called photon or quanta with a concentration of 0.250 g/L and
(A) First absorptivity of 0.584 at 280 nm
(B) Second (A) 0.1460
(C) Third (B) 0.4281
(D) Fourth (C) 2.336
(D) 40.88
4. Region(s) in the electromagnetic spectrum
(A) Visible 12. A 0.0005 M solution of analyte is placed in a
(B) Ultraviolet sample cell with a path of 1.09 cm. When
(C) Infrared measured at 350 nm, the absorbance was
(D) AOTA 0.139. Compute for the molar absorptivity
(A) 0.1275 / mol●L
5. It is the number of complete cycles that pass a (B) 3.9201 / mol●L
given point per second (C) 75.755 / mol●L
(A) Wavelength (D) 255 / mol●L
(B) Spectrum
(C) Radiant energy 13. The proportion of light that reaches the
(D) Frequency sample is known as "transmittance" and is
represented by this/these equation(s)
6. Energy transition present in IR region (A) T = log Ps/Pb
(A) Ee (B) %T = Ps/Pb x 100
(B) Ev (C) %T = Pb/Ps
(C) Er (D) T = Pb/Ps x 100
(D) Et
14. A plot of absorbance against a concentration
7. It refers to the energy in the electromagnetic of a standard drawn in straight line is
spectrum that is propagated in waveform (A) Charles'
(A) Radiant energy (B) Lambert's
(B) Electrical energy (C) Beer's
(C) Transmittance (D) B & C
(D) Frequency
15. The MIR region of the spectrum used to
8. The following are true regarding energy, identify a substance has a wavelength range
except of
(A) E = hv (A) 200-380 nm
(B) E = hc/v (B) 780-3000 nm
(C) E = hc/l (C) 380-780 nm
(D) E = hcv (D) 3-15 um

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 1

16. If the wavelength of light is 10-7 cm, this is 23. A radiationless process when the downward
equal to one _________ transitions occur at a rate greater than the
(A) um upward transitions, resulting to the
(B) Angstrom appearance of useful NMR signals
(C) nm (A) Saturation
(D) mm (B) Relaxation
(C) Resonance
17. Which of the following components of energy (D) Angular rotation
is not involved in spectroscopy?
(A) Electronic 24. Also known as flame photometry
(B) Rotational (A) Atomic Absorption Spectrophotometry
(C) Vibrational (B) Atomic Emission Spectrophotometry
(D) Translational (C) Fluorometry
(D) Mass Spectroscopy
18. Which of the following is true about
spectroscopy? 25. Radiant energy required in the analysis of
I. A method of analysis dealing with the drugs under fluorometry is in
measurement of spectra (A) UV
II. Measure the wavelength in the (B) Visible
electromagnetic spectrum where the (C) IR
radiant energy has interacted with a (D) A & B
chemical species
III. Measure the power of transmitted, 26. Substances that have the power of rotating
fluorescent, reflected or emitted light the plane, polarized light are said to be
(A) I, II (A) Light-sensitive
(B) II, III (B) Active constituents
(C) I, III (C) Optically active
(D) I, II, III (D) Dextrorotatory
(E) I
27. It relies on the production of ions from a
19. A branch of spectrometry which deals with the parent compound and the subsequent
measurement of the brightness of light characterization of the patterns that are
reflected by a turbid solution produced
(A) Turbidimetry (A) Turbidimetry
(B) Mass Spectroscopy (B) Mass Spectroscopy
(C) Nephelometry (C) Nephelometry
(D) Fluorometry (D) Fluorometry

20. Limit test for Ca2+, Na+, and Cl- employs 28. In this technique, the transmitted light is
(A) HPLC measured after radiant energy passes
(B) Membrane filtration through a turbid solution or suspension
(C) UV-VIS spectrophotometry (A) Nephelometry
(D) Flame photometry (B) Turbidimetry
(C) AES
21. Which of the following is not true about NMR? (D) AAS
I. C-13 is the most commonly used,
highly abundant and most sensitive 29. The following are optical methods of analysis,
isotope except
II. Sample probe is very small (A) Colorimetry
III. Very sensitive analytical technique (B) Chromatography
IV. TMS is used as a reference standard (C) Nephelometry
(A) I, II (D) Spectrophotometry
(B) I, III
(C) II, IV 30. The reading that must be obtained in a
(D) II, III, IV spectrophotometer
(A) Concentration error
22. Which of the following solvents is not used in (B) Retention
NMR studies? (C) Absorbance
(A) CCl4 (D) Angular rotation
(B) CHCl3
(C) CDCl3 31. An instrument in spectrometry that doesn’t
(D) CS2 have prism or grating device
(A) Filter photometer
(B) UV-Vis spectrophotometer
(C) IR spectrophotometer
(D) Flame photometer

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 2

32. A spectrophotometer differs from a (D) NaCl
colorimeter because it consists of
(A) Prism 41. In IR spectrometry, water is not used as a
(B) Lamp house solvent for the sample because the water will
(C) Cell compartment (A) Absorb IR radiation
(D) Optical scale (B) Not dissolve the sample
(C) Dissolve the NaCl holder
33. In spectrophotometry procedure for assay, (D) A & C
aside from the sample or unknown, this is also
required 42. The following are classifications of
(A) Raw material of the sample chromatographic methods, except
(B) Dosage form (A) Adsorption chromatography
(C) Reference standard (B) Absorption chromatography
(D) A & C (C) Size-exclusion chromatography
(D) Ion-exchange
34. Defined as the width of the segment of the
spectrum that is isolated by a monochromator 43. Gypsum is added to silica as adsorbent in
(A) Exit slit chromatography to serve as
(B) Entrance slit (A) Hardening agent
(C) Bandpass (B) Thickening agent
(D) Cuvette (C) Binder
(D) Lubricant
35. Part of the spectrophotometer that is used to
isolate the desired wavelength 44. Determine the distance travelled by the solute
(A) Cuvette if its Rf value is 0.688 and the solvent travels
(B) Monochromator 8 cm. The sample weight is 1.02 mg
(C) Lamps (A) 11.6 cm
(D) Detector (B) 11.8 cm
(C) 5.5 cm
36. It measures the magnitude of the current (D) 0.7 cm
generated by a detector
(A) Read-out device 45. The common adsorbent used in TLC is
(B) Monochromator (A) Cellulose
(C) Lamps (B) Silica
(D) Detector (C) Alumina
(D) Charcoal
37. Photodiode or photomultiplier tube is used in
UV-Vis spectrophotometer as a 46. The visual output/result in chromatography
(A) Radiation source (A) Chromatogram
(B) Monochromator (B) Chromatography
(C) Detector (C) Chromatograph
(D) Read-out device (D) A & C

38. Using a spectrophotometer to measure the 47. In thin layer chromatography, finding the spot
concentration of a sample, the following data of the colorless compound in the
were obtained: absorbance (A) of the chromatogram can be done by
standard solution was 0.361; A of the sample (A) Use of H2SO4 spray
was 0.356 and concentration of the standard (B) Use of CCl4 spray
used was 0.068 mg/mL. The concentration of (C) Use of UV radiation
the sample was (D) A & B
(A) 5.29 mg/mL
(B) 1.89 mg/mL 48. HPLC is the method of choice for the analysis
(C) 0.067 mg/mL of
(D) 0.069 mg/mL (A) Substance with high polarity and
molecular weight
39. Ways to prepare the sample for infrared (B) Non-volatile substance
determination, except (C) Volatile substance
(A) Use of mull technique (D) A & B
(B) Use of KBr pellet
(C) Use of alcohol as solvent 49. In GC, the result of assay is expressed in
(D) Use of liquid petrolatum as solvent (A) Retention in the column
(B) Rf value
40. Most commonly used matrix in pellet making (C) Volume of the gas used
(A) CaCl (D) Adsorbent
(B) KBr
(C) KCl

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 3

50. The type of chromatography where the 58. The microorganism used in the microbial assay
cellulose of the filter paper is used as the for Vitamin B12
adsorbent (A) Lactobacillus shirota
(A) Column (B) Lactobacillus plantarum
(B) Paper (C) Lactobacillus leichmanii
(C) TLC (D) Lactobacillus bifidus
(D) Gas
59. The calcium and sodium content of the blood
51. Chromatographic procedure in which non- can be determined by
polar solvent acts as the stationary phase and (A) Flame spectroscopy
a polar solvent used as the mobile phase (B) Colorimetry
(A) Adsorption (C) Nephelometry
(B) Partition (D) Turbidimetry
(C) Reversed-phase
(D) Ion exchange 60. Potentiometry find application in
(A) Biologic assay
52. The spotting agent for amino acids in paper (B) Qualitative analysis
chromatography is (C) pH determination
(A) Potassium chromate (D) A & B
(B) Ninhydrin
(C) Silver nitrate 61. Refractive index varies with this parameter
(D) Iodine therefore it should be held constant
(A) Temperature
53. The heart of HPLC and GC (B) Volume
(A) Pump (C) Wight
(B) Column (D) Wavelength
(C) Injection valve
(D) Detectors 62. Used to measure water content of raw
materials
54. The inert gas used as a mobile phase in gas (A) Karl Fischer titrimeter
chromatography is referred to as (B) Pycnometer
(A) Vehicle gas (C) Platform balance
(B) Carrier gas (D) NOTA
(C) Stationary phase
(D) Fixed phase 63. A water content determination method that
uses xylene tube
55. Stationary phase in gas chromatography (A) Gravimetry
(A) Gas (B) Karl Fischer titrimetry
(B) Liquid (C) Azeotropic method
(C) Cellulose (D) Dew point method
(D) Chromatogram
64. The primary standard used to standardize
56. Which of the following statement(s) is/are Karl Fischer reagent is
true? (A) Sodium carbonate
I. Chromatography is used to separate (B) Potassium biphthalate
the components of a mixture (C) Sodium tartrate
II. Mobile phase is the substance fixed (D) Sodium oxalate
in place for the chromatography
procedure 65. Which of the following chemicals is not
III. Stationary phase carries the sample included in preparing the Karl Fischer
through the mobile phase reagent?
(A) I, II, III (A) Pyridine
(B) II, III (B) Acetone
(C) I (C) SO2
(D) II (D) Iodine
(E) III
66. What is the reference electrode used in
57. The ratio of the velocity of light in air to the polarography?
velocity of light in the medium (A) Saturated hydrogen
(A) Rf value (B) Dropping mercury
(B) Refractive index (C) Glass
(C) Specific gravity (D) Saturated calomel
(D) Density

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 4

 II. PRODUCT TESTING 75. Determine the shelf-life of a product
(A) Sampling inspection program
67. Torque test in an example of (B) Validation program
(A) Container control (C) Stability testing program
(B) Closure control (D) AOTA
(C) Leak test
(D) Volatilization test 76. In the microbial assay of antibiotic, the
microbe used for Penicillin G is
68. One of the following is not an in-process (A) Bacillus subtilis
control for tablets (B) Pseudomonas aeruginosa
(A) Bioavailability (C) Staphylococcus aureus
(B) Hardness & thickness (D) NOTA
(C) Weight variation
(D) Disintegration 77. High Efficiency Particulate Air is an example
of
69. Which are properly matched? (A) mill
I. Digitalis: Pidgeon (B) dryer
II. Cat: Glucagon (C) filter
III. Dog: PTH (D) sprayer
IV. Chicken: Oxytocin
(A) I, II, III, IV 78. Friability testing is done using a Roche
(B) II, III, IV friabilator that must be set to ensure
(C) I, III, IV (A) 10 falls of tablets
(D) II, IV (B) 100 falls of tablets
(C) 10 rpm
70. An instrument used to measure the durability (D) Either A or B
of tablets to shipping/transportation against
shock and abrasion 79. The minimum time period covered by the date
(A) Metal detector do accelerated stability studies is
(B) Friabilator (A) 6 months
(C) Top loading balance (B) 12 months
(D) Colorimeter (C) 18 months
(D) NOTA
71. In what type of dosage form is the
determination of zeta potential needed? 80. A test which is determined by selecting NMT
(A) Suspension 30 tablets from each production batch and
(B) Emulsion assaying 10 tablets individually as directed
(C) Aerosol in the assay of official monographs
(D) Solutions (A) Weight variation test
(B) Friability test
72. Methods of microbial assay for antibiotics (C) Disintegration test
(A) Turbidimetric (D) Content uniformity test
(B) Cylinder plate
(C) A & B 81. The sterilizing efficiency of an autoclave is
(D) NOTA determined by using which of the following
biological indicators?
73. What is the USP limit for content uniformity? (A) Bacillus subtilis
(A) 85 – 115% (B) Bacillus anthracis
(B) 92.50 – 107.50% (C) Bacillus stearothermophilus
(C) 90 – 110% (D) Bacillus cereus
(D) 95 – 105%
82. Given a tablet thickness of 0.47 cm, what
74. In the 1st stage of disintegration test, if 1 or 2 should be the acceptable range?
fails to disintegrate completely, the next step (A) 0.4465 – 0.4935 cm
is (B) 0.4348 – 0.5050 cm
(A) Repeat the test using additional 12 (C) 0.4320 – 0.5170 cm
samples, 16 out of 18 samples must (D) 0.3525 – 0.5875 cm
completely disintegrate
(B) Repeat the test using 20 samples, all 83. The dye used in leaker's test is
samples should completely disintegrate (A) Methylene blue
(C) Repeat the test twice and determine the (B) Thymol blue
average disintegration time (C) Phenolphthalein
(D) Reject the samples (D) NOTA

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 5

84. Which is used to predict the shelf-life of a (C) 18
product? (D) 3
(A) Michaelis-Menten
(B) Arrhenius 94. The microbial assay for this compound is
(C) Hixson-Crowell based on the inability of the microbe to
(D) Stokes synthesize the factor being assayed
(A) Antibiotics
85. Safety and toxicity tests are conducted using (B) Vitamins
(A) Rabbits (C) Insulin
(B) Dogs (D) Sulfonamides
(C) White mice
(D) Guinea pig 95. LAL test results could be determined based on
I. Color development
86. For the disintegration of enteric-coated II. Cloudiness
tablets, the immersion fluid is/are III. Gel clot formation
(A) Distilled water (A) I, II
(B) Simulated intestinal fluid (B) II, III
(C) Simulated gastric fluid (C) I, III
(D) B & C (D) I, II, III

87. The determination of average weight of a 96. In disintegration test, the temperature of the
batch is performed on ___ tablets medium is usually
(A) 9 (A) 32 ± 2°C
(B) 12 (B) 35 – 39°C
(C) 18 (C) 37 ± 0.5°C
(D) 20 (D) 37 ± 2°C

88. Content uniformity test is used to ensure which 97. Tablet friability test is also known as
of the following quality in tablet products (A) Tensile strength
(A) Disintegration (B) Brittleness index
(B) Bioavailability (C) Drop test
(C) Purity (D) Abrasion test
(D) Potency
98. A helix, non-reactive material that keeps the
89. The operating speed for paddle in dissolution solid dosage for mat the bottom of the vessel
apparatus during dissolution tests
(A) 50 rpm (A) Disk
(B) 100 rpm (B) Cylinder
(C) 150 rpm (C) Sinker
(D) 200 rpm (D) Basket

90. Given the average tablet weight of 125 mg, 99. An enzyme used in ELISA test kit reagent
what should be the acceptable tablet weight (A) Tetramethylbenzidine
range? (B) Alkaline phosphatase
(A) 117 – 143 (C) Sulfuric acid
(B) 112.5 – 137.5 (D) Phosphate buffered saline
(C) 115.63 – 134.38
(D) 123.75 – 126.25 100. Which tablet attribute is correlated with the
biological activity of the active ingredient?
91. Initial number of samples that used in Rabbit (A) Disintegration
Pyrogen Test (B) Dissolution
(A) 8 (C) Content uniformity
(B) 6 (D) NOTA
(C) 5
(D) 3 101. The plunging and raising of movement of the
basket rack assembly in a disintegration
92. Bleeding is a main stability problem seen in apparatus moves at a rate of
(A) Capsules (A) 25 – 28 cycles/min
(B) Emulsion (B) 23 – 30 cycles/min
(C) Ointments (C) 29 – 32 cycles/min
(D) Tablets (D) 50 – 100 cycles/min

93. The number of samples used per run of

disintegration test
(A) 12
(B) 6

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 6

102. Test method of choice (FDA & USP) for sterility III. Sandwich ELISA: Ab → Ag →
of parenterals Labelled Ab → Substrate
(A) Direct transfer method (A) I, II, III
(B) Test tube inoculation (B) I, II
(C) Membrane filtration method (C) II, III
(D) Bacterial endotoxin test (D) III

103. The microbial assay of cyanocobalamin uses 111. Pore size of membrane filter used in sterility
which organism? testing
(A) Lactobacillus plantarum (A) 10 um
(B) Lactobacillus shirota (B) 0.25 um
(C) Lacrobacillus aureus (C) 0.35 um
(D) Lactobacillus leichmanii (D) 0.45 um

104. Most common enzyme used in ELISA due to its 112. Minimum requirement for satisfactory tablet
greater sensitivity hardness
(A) Alkaline phosphatase (A) 4 kg/inch2
(B) Horseradish peroxidase (B) 8 kg/inch2
(C) Lactoperoxidase (C) 10 kg/inch2
(D) β-galactosidase (D) 12 kg/inch2

105. What would be the allowable variation in 113. It was suggested that inspectors in clarity test
tablet diameter if the standard tablet should see particles as mall as what size?
measures 12 mm? (A) 20 mm
(A) ± 3% (B) 10 mm
(B) ± 5% (C) 20 um
(C) ± 2% (D) 10 um
(D) ± 1%
114. The first line of apparatus in the product
106. Bacterial Endotoxin Test (BET) method is also development of controlled-release
known as formulations
(A) Safety test (A) Apparatus 4
(B) Sterility test (B) Apparatus 3
(C) LAL method (C) Apparatus 2
(D) Microbial Assay (D) Apparatus 1

107. Tracer gas approved by the FDA in leaker 115. Apparatus 7 is formerly known as
detection of blister/strip packs (A) Reciprocating Cylinder
(A) Oxygen (B) Reciprocating Disk
(B) Helium (C) Reciprocating Holder
(C) Nitrogen (D) Reciprocating Basket
(D) CO2
116. The sodium d light has a wavelength of
108. The temperature at which the dissolution test (A) 586 nm
of transdermal patches and ointments are (B) 587 nm
conducted (C) 588 nm
(A) 32 ± 2°C (D) 589 nm
(B) 32 ± 0.5°C
(C) 37 ± 2°C 117. Equipment used for tablet hardness test that
(D) 37 ± 0.5°C operates on pneumatic or air pressure
mechanism
109. The accepted weight loss in friability test (A) Stokes-Monsanto
should be (B) Pfizer
(A) NMT 1% (C) Erweka
(B) NLT 1% (D) Strong-Cobb
(C) NMT 8%
(D) NLT 3% 118. Coning due to poor hydrodynamics is a
common problem encountered with
110. Which of the following statement(s) is/are (A) Apparatus 1
correct? (B) Apparatus 2
I. Direct ELISA: Ag → Primary Ab → (C) Apparatus 3
Labelled Ab → Substrate (D) Apparatus 4
II. Reverse ELISA: test multiple samples
in a single well

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 7

119. Most preferred medium in dissolution testing
(A) Alcohol
(B) Ether
(C) Acetone
(D) Distilled Water

120. Chloramphenicol is normally paired with what

organism in microbial assays?
(A) S aureus
(B) E coli
(C) K pneumoniae
(D) B subtilis

121. LAL is obtained from

(A) Rabbits
(B) White mice
(C) Horseshoe crab
(D) Microorganism

122. This is the acceptable hardness range for

chewable tablets
(A) 4 – 10 kg
(B) 5 – 8 kg
(C) 2 – 3 kg
(D) 2 – 8 kg

123. A parenteral is declared to be pyrogenic id

the total rise in temperature of the rabbit is
(A) Less than 3.3°C in the 8 rabbits
(B) Less than 3.4°C in the 8 rabbits
(C) More than 3.3°C in the 8 rabbits
(D) B & C

124. Amount of dissolved drug can be determined

by
(A) Analytical balance
(B) Brookfield viscometer
(C) Dissolution tester
(D) DOP spray

The relationship of absorbance to concentration is

expressed in the equation known as Beer's law:

A=abC
Where:
A = absorbance
a = absorptivity coefficient (constant)
b = length of light path (constant)
C = concentration

PHARMACEUTICAL ANALYSIS II /// Justin Dave M. Manantan, RPh 8