r05222302 Instrumental Methods of Analysis

Code No: R05222302 Set No.
1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) Define the terms qualitative & quantitative analysis.

(b) Differentiate between micro & semi micro analytical methods.
(c) What are advantages & limitations of chemical methods. [6+6+4]
2. Compare & contrast the techniques phase -contrast microscopy & differential in-
terference contrast microscopy (DIC) [16]
3. Explain the following terms:
(a) Gravitational force.

(b) Centrifugal force
(c) Sedimentation coefficient. [5+5+6]
4. You have two samples of DNA derived from different organisms,A & B. DNA from
A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA
from B shows a corresponding increase in O.D beginning at 650 C & plateau out
at 760 C. What is your conclusion. Justify your answer. [16]
5. (a) Define fluorescence. Derive an expression relating intensity of fluorescence &

concentration.
(b) Write a brief note on :
i. Triplet state
ii. Doublet state
iii. Singlet state. [8 × 2 = 16]
6. (a) Discuss the basic principle of infra red spectroscopy?

(b) Briefly describe the scanning of infrared spectrum of an organic compound.
[8 × 2 = 16]
7. Explain the following :
(a) Free inductive decay

(b) Hetero nuclear decoupling
(c) Off resonance coupling
(d) Chemical shift. [8 × 2 = 16]
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Code No: R05222302 Set No. 1
8. Give an account on electronic structure & hyperfine splitting in ESR spectroscopy?
[16]
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(Bio-Technology)
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1. Give a brief account on methods for reporting analytical data? [16]
2. Compare & Contrast the optical magnification obtained by the bright & dark field
microscopy along with their specific applications. [16]
3. (a) Give the basic principle involved in analytical centrifugation.

(b) What are the important application of this technique in biochemistry?
[8 × 2 = 16]
4. You have two samples of DNA derived from different organisms,A & B. DNA from
A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA
from B shows a corresponding increase in O.D beginning at 650 C & plateau out
at 760 C. What is your conclusion. Justify your answer. [16]
5. (a) Define optical activity? What is the principle involved in circular dichroism
spectroscopy?
(b) Differentiate the polarimetry & circular dichroism. [8 × 2 = 16]
6. Discuss the principle, importance & applications of mass spectrometry? [16]
7. (a) Give the principle underlaying in NMR spectrometry?

(b) Describe the component of a NMR spectrometer with a neat labeled diagram.
[8 × 2 = 16]
8. (a) What is the principle of ESR. Discuss some important applications of ESR.
(b) What are the limitations of ESR. [8 × 2 = 16]
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(Bio-Technology)
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1. Give an account of the instruments involved in electrochemical methods of analysis

along with their principle of working. [16]
2. (a) Give the basic principle involved in the viewing an object in dark field.
(b) Draw a neat labeled diagram of optical pathway of dark field microscope.
(c) Give its specific use. [5+6+5]
3. (a) Give the basic principle involved in analytical centrifugation.

(b) What are the important application of this technique in biochemistry?
[8 × 2 = 16]
4. What are the different regions of the electromagnetic spectrum & where are they
used for? [16]
5. Define the following terms:
(a) chiral compounds

(b) cotton effect
(c) circular birefringence. [6+5+5]
6. How will you distinguish between intermolecular & intramolecular hydrogen bond-
ing by IR spectroscopy? [16]
7. (a) Explain the principle involved in CMR spectra?

(b) Why it is not possible to determine relative ratio of carbon atoms in a com-
pound by integration of peak areas in 13C NMR as in PMR?. [8 × 2 = 16]
8. Why ESR spectroscopy is widely used in the study of chemical, photochemical &
electrochemical reactions? Justify your answer. [16]
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(Bio-Technology)
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1. Write an account on analytical methods based on the physical property measure-

ment? [16]
2. Narrate the principle of operation of a fluorescence micrscope. Give its applications

in biology? [5+11]
3. Calculate the molecular mass of the protein where the sedimentation coefficient of
the protein is 7.75 ×10−13 s. In subsequent analysis the protein was found to have
an average diffusion coefficient 0f 4.0 × 10−11 m2 s−1 & the a partial specific volume
of 0. 734 ×10−3 m3 kg −1 .
(a) convert the molecular mass to relative molecular mass.

(b) Express the molecular mass in Daltons. [8 × 2 = 16]
4. Define the terms:
(a) i. Radiant power( P )

ii. Path length of radiation
iii. absorptivity
iv. molar absorptivity.
(b) Describe & derive the Beer’s law.
(c) What are its limitations & deviations. [4+6+6]
5. (a) What is atomic absorption. What are its advantages & disadvantages.
(b) Give some of its limitations. [8 × 2 = 16]
6. (a) What are the different regions of infrared radiation?

(b) Explain various types of stretching & bending vibrations with suitable exam-
ples. [8 × 2 = 16]
7. (a) Why does a signal for a particular set of protons is split into a multiplet.
(b) explain with suitable examples the trem spin-spin coupling [8 × 2 = 16]
8. (a) Which of the following two formulas is correct for the complex obtained be-
tween copper ion & 8 quinolinethanol (C9 H7 N5 )
i. Cu(I) (C9 H7 N5 )(C9 H6 N5 )
ii. Cu(II) (C9 H6 N5 )2
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show that the ESR approach is appropriate for this determination.
(b) Which is one of the best known free radicals used in calibrating ESR spectra?
[8 × 2=16]
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Code No: R05222302 Set No.

1. (a) Define the terms qualitative & quantitative analysis.

3. Explain the following terms:

(a) Gravitational force.

5. (a) Define fluorescence. Derive an expression relating intensity of fluorescence &

6. (a) Discuss the basic principle of infra red spectroscopy?

7. Explain the following :

(a) Free inductive decay

1. Give a brief account on methods for reporting analytical data? [16]

3. (a) Give the basic principle involved in analytical centrifugation.

6. Discuss the principle, importance & applications of mass spectrometry? [16]

7. (a) Give the principle underlaying in NMR spectrometry?

1. Give an account of the instruments involved in electrochemical methods of analysis

3. (a) Give the basic principle involved in analytical centrifugation.

5. Define the following terms:

(a) chiral compounds

7. (a) Explain the principle involved in CMR spectra?

1. Write an account on analytical methods based on the physical property measure-

2. Narrate the principle of operation of a fluorescence micrscope. Give its applications

(a) convert the molecular mass to relative molecular mass.

4. Define the terms:

(a) i. Radiant power( P )

6. (a) What are the different regions of infrared radiation?

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