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OPERATION

MANUAL
OM2438
Revision 6

Centra-MP4
Centrifuge
Cat. No. 2437 -- For 100/120/220/240 VAC, 50/60 Hz

Centra-MP4R
Refrigerated Centrifuge
Cat. No. 2438 -- For 100/120/220/240 VAC, 50/60 Hz

International Equipment Company


300 Second Ave.
Needham Heights, MA USA
Tel. +(781) 449-8060 Fax +(781) 444-6743
Table Of Contents

1 INTRODUCTION ........................................................................................................................................ 2
2 INSTALLATION ......................................................................................................................................... 3
2.1Receiving the Unit ............................................................................................................................... 3
2.2 Site Preparation .................................................................................................................... 3
Clearance Envelope ....................................................................................................... 3
2.3 Power Configuration .............................................................................................................. 3
Voltage .......................................................................................................................... 4
Line Frequency .............................................................................................................. 4
Power Cord .................................................................................................................... 4
2.4 Moving the Unit ..................................................................................................................... 5
2.5 The Front Panel .................................................................................................................... 5
3 OPERATION ..................................................................................................................................... 8
3.1 Rotor and Accessories .......................................................................................................... 8
Rotor Balance ................................................................................................................ 9
Vibration ........................................................................................................................ 9
Rotor Installation .......................................................................................................... 10
Rotor Removal ............................................................................................................. 11
Adding Rotors .............................................................................................................. 11
Deleting Rotors ............................................................................................................ 11
3.2 Starting and Stopping a Run ................................................................................................ 11
Manual Operation ......................................................................................................... 12
Momentary Mode ......................................................................................................... 12
Hold Mode ................................................................................................................... 12
Timing Mode ................................................................................................................ 13
3.3 Stored Programs ................................................................................................................. 13
Locking Programs ........................................................................................................ 13
Recall Program ............................................................................................................ 13
Add/Change Program ................................................................................................... 13
3.4 Centra MP4R Refrigeration .................................................................................................. 14
Rapid Condition ........................................................................................................... 14
3.5 Warning Messages and Error Codes ................................................................................... 15
4 APPLICATIONS ................................................................................................................................... 17
Corrosive Solvents ....................................................................................................... 17
4.1 Speed and Force Tables ..................................................................................................... 18
4.2 Derating Tables ................................................................................................................... 25
Dense Samples ........................................................................................................... 25
244 Rotor ..................................................................................................................... 25
4.3 Chemical Resistance Table ................................................................................................. 27
4.4 Decontamination Table ........................................................................................................ 28
4.5 RCF Nomograph ................................................................................................................. 29
5 MAINTENANCE ................................................................................................................................... 30
5.1 Cleaning ............................................................................................................................. 30
Corrosion ..................................................................................................................... 31
Storage ....................................................................................................................... 31
Decontamination .......................................................................................................... 31
5.2 Cover Interlock Bypass ....................................................................................................... 32
5.3 Calibration .......................................................................................................................... 32
5.4 Brush Replacement ............................................................................................................ 33
5.5 Table of Spare Parts ........................................................................................................... 34
5.6 Warranty ............................................................................................................................ 34
5.7 Condition of Returned Equipment ......................................................................................... 35
5.8 Fuses Not Replaceable By The Operator ............................................................................. 35
6 SPECIFICATIONS ................................................................................................................................... 36

Centra MP4/MP4R Operation Manual 1


1 INTRODUCTION

The Centra MP4 and Centra MP4R are high-speed, multi-purpose


centrifuges used in medical, industrial, and scientific laboratories. These
bench-top units can achieve centrifugal forces of up to 16,750xg (using the
852 rotor), and speeds up to 14,000 rpm (using the 817 rotor). The Centra
MP4R provides refrigerated temperature control.

Both units accommodate a wide variety of rotors, including angle, swinging


bucket, and fixed horizontal designs. They can process tubes, bottles,
microplates, microcapillary tubes, cytological slide carriers, and microsample
tubes. The units can centrifuge up to 1 liter of fluid in a single operation.

An easy-to-use front panel provides many versatile modes of operation:


manual and pre-programmed operation, momentary spin, indefinite spin
(hold mode), and a Rapid Condition (MP4R Only) function for pre-cooling or
pre-warming the rotor and sample chamber. You can select gentle
acceleration and deceleration rates to maintain samples with density
gradients. In addition, rotor number entry allows direct G-force control
through automatic calculation of RCF. With the MP4R, you can select any
chamber temperature down to -5°C. Repeat runs with precisely the same
temperature, speed, and time settings may be achieved at the touch of a
button.

The internal microprocessor simplifies operation, ensures repeatable results


for continued success, and alerts operators when periodic maintenance is
due.

A fail-safe cover interlock ensures that the cover is closed before a run can
begin, and prevents the cover from opening until the rotor has slowed to a
safe speed, even if the power fails. The rugged steel cabinet and rigid
construction provide quiet operation and long-term reliability. A run
automatically aborts at a safe, low speed if the rotor is unbalanced.

Both units are easily configured to many different AC power sources, have
CSA certification, and are designed to meet requirements for UL listing, IEC
1010, British Standard 4402 and other international standards.

2 Centra MP4/MP4R Operation Manual


2 INSTALLATION

2.1 Receiving the Unit


IEC ships the centrifuge in a carton that protects it from shipping hazards.
Follow the unpacking instructions on the carton. Be sure to complete the
postage-paid warranty card and return it to IEC.

2.2 Site Preparation


The Specifications in Section 6 of this manual give the dimensions of the
unit. Provide clearance of 8 cm (3 inches) on each side of the unit for
ventilation. Place the unit on a clean and dry surface to ensure that the
suction feet grip the surface firmly. Clear the area beneath the unit of debris
and loose materials such as paper.

Clearance Envelope International Electrotechnical Commission standard 1010 part 2-20


limits the permitted movement of a laboratory centrifuge to 300mm in the
event of a disruption. The user should therefore mark the clearance
envelope boundary around the centrifuge, or laboratory management
procedures should require that no person or any hazardous materials are
within such a boundary while the centrifuge is operating.

The surface must be level to ensure quiet, vibration-free operation. A rigid,


stable location is important since an improperly-loaded unit can vibrate or
even move.

2.3 Power Configuration


The Centra-MP4R and Centra-MP4 use AC power at 100, 120, 220, or 240
Volts (nominal), at 50 or 60 Hz. The unit is shipped without fuses installed
and must be configured for power at your site. Do not plug in the
centrifuge until you have configured it correctly. For best results, the
centrifuges should be used on a dedicated line. Variations in line voltage or
frequency will affect the unit’s speed and other characteristics. Less than
nominal line voltage may prevent the centrifuge from reaching maximum
published specifications of speed and/or temperature. Also, power line
voltage at some locations may sag when the refrigeration system turns on.

Centra MP4/MP4R Operation Manual 3


Caution: configuring the centrifuge incorrectly will void your
warranty.

Voltage Locate the power entry module on the lower left side of the unit
(Figure 2-1). On the right side of this module is the fuse drawer
(Figure 2-2). A small latch on the left holds the drawer in place.
Press the latch and slide the drawer out. When removing the fuse
drawer, be careful that the fuses do not fall out.

Fuse Drawer Power Entry Module

Latch

Fuse
Fuse
Drawer

Remove and
Rotate for 240 Voltage
Proper Voltage Selection
Window
240

220
100
120

If the number visible in the window differs from the voltage at your
site, remove the square insert, rotate it, and insert it so that the
correct voltage is displayed through the window. (100, 120, 220, or
240 Volts. See Section 6 Specifications for operable voltage ranges.)

Install appropriate fuses for the voltage at your site as follows:


For 100/120 V, install the two 16 A fuses. (IEC Part No. 50058)
For 220/240 V, install the two 6.3 A fuses. (IEC Part No. 9946)
Ensuring that the fuses are securely in place, reinstall the fuse
drawer in the side of the centrifuge.

Line Frequency (Centra-MP4R Only). Locate the 50Hz/60Hz switch to the right of the
power receptacle. Adjust this switch with a tool(screwdriver) to match
the frequency at the site.

Power Cord The unit requires a grounded power supply (3-prong power outlet).If
your facility does not have properly-grounded power outlets, arrange
for proper grounding.

4 Centra MP4/MP4R Operation Manual


IEC provides two power cords with each Centra-MP4R and Centra-MP4.
One is suitable for North America and Japan. The second has bare wires at
one end for attachment of other types of plugs.

Caution: Do not remove the grounding pin from the centrifuge power
cord. Do not use the bare wired power cord to attach a power plug that
does not have a grounding pin.

When using the power cord with bare wires, install any required plug and
attach the cord to the receptacle on the lower left side of the centrifuge. Plug
into the power outlet.

Warning: The power cord(s) provided with the unit is correctly rated for
the highest current demand. This power cord should not be
interchanged with cords from equipment with lower current demand.
Exchange of power cords between equipment may create a fire hazard.

2.4 Moving the Unit


Suction cups adhere the unit to the work surface and keep it from moving.
This is a safety feature. To move the unit to another location, use an object
such as a tongue depressor under each suction cup. Lift up an edge of each
suction cup and insert the object far enough to break the vacuum seal of that
cup. When all four cups are disengaged, the unit can be easily lifted. When
the unit is in its new location, ensure that the suction cups are operating
properly again. Be sure the new location is appropriate (see Section 2.2).

The voltage and frequency settings may need to be changed at the new
location. Check carefully that the unit is configured for the correct voltage
and frequency. If necessary follow the instructions in Section 2.3.

2.5 The Front Panel


The On/Off key must be On to enable use of the front panel. This key
controls power to the refrigeration system (MP4R)and the display. The unit
displays the chamber temperature whenever the unit is plugged in.
The On/Off key is inoperative during a run; to shut off the refrigeration
system, first stop the run by pressing the STOP key.

The front panel contains numeric displays labeled °C (MP4R only), RPM/
RCF, ROTOR/RADIUS, and MINUTES. Normally, the numeric displays
indicate actual readings of temperature, rotor speed, and the elapsed time of,
or time remaining in, the run. The display instead indicates the desired
settings for the run:

(1) whenever the up and down arrow keys are used,


(2) briefly at the start of a run, and
(3) briefly after the unit is switched on.

When the display shows actual readings, the numbers are bright; when the
display shows desired settings, the numbers are dim.

Centra MP4/MP4R Operation Manual 5


The number under this symbol represents temperature in whole degrees
Celsius, from -5° C through 45° C (Model MP4R, only).

The number under this symbol represents the rotor speed in RPM or the
Relative Centrifugal Force (RCF) in xg.

RPM This key toggles between RPM and RCF. When RPM is selected, the speed
RCF indicator displays revolutions per minute. When RCF is selected, the speed
indicator displays relative centrifugal force, and the rotor/radius indicator
displays either the rotor number or the rotor radius. RCF is only indicated
when a rotor number has been selected in the rotor/radius display.

The number under this symbol displays time. Times of less than 10 minutes
are displayed as [mm:ss]. Times of 10 minutes or longer are displayed as
minutes. Time can be set up to 360 minutes. In the hold mode, times up to
999 minutes will be displayed. Times greater than 999 minutes are displayed
by alternately flashing hours, shown as H[hh], and minutes past the
displayed hours, shown as [mm].

The arrow keys beneath each display are used to view or change the desired
settings for temperature (MP4R only), rpm/rcf, rotor/radius, or time. The first
time the key is pressed, the numeric display simply switches from actual
readings to desired settings, without changing the desired settings. When
pressed again, the selected setting increases or decreases once for each
press. If the key is held in, the setting changes continuously until the key is
released. The longer it is held in, the more rapidly the setting changes.
Usually, the key is held in to approach a desired setting, then pressed
repeatedly to select the exact setting. Three seconds after the arrow key is
released, the display returns to the actual readings.

This key starts a run. The run is governed by the desired settings shown on
the numeric display. The associated green light blinks until the rotor
approaches the desired run speed. Then the light stays on until the end of
the run.

This key stops the run. (A run will also stop automatically when the desired
time has elapsed.) The associated red light blinks as the rotor decelerates.
(It also blinks if an error occurs; see Section 3.5.) When the run ends, the
light stays on.

This key unlocks the cover. This key is inoperative if a run is in progress;
pressing it does not stop the run. The cover will not unlock until the rotor
speed is less than 100 RPM. The associated yellow light is on whenever the
cover is open. A run cannot be started when the cover is open.

This key saves the currently displayed desired settings as stored program 0
through 9 (see Section 3.3). The numeric display above it shows the stored
program number or other mode of operation (see Section 3.2). The numeric
displays can also display warning or error messages (see Section 3.5).

6 Centra MP4/MP4R Operation Manual


The rotor/radius display indicates either the selected rotor number or the
rotor radius in centimeters. This display illuminates when the rotor is
selected. The applicable IEC rotor numbers are supplied in the memory,
along with their maximum radius in centimeters. The key under rotor/radius
toggles between the two. To select a rotor number, toggle to ROTOR and
press an arrow key under the rotor display. To change the radius, toggle to
RADIUS, and press an arrow key under the rotor display. Note that the
radius cannot be changed to a radius larger than the maximum radius for that
rotor. The display changes back to rotor number after three seconds.

Gentle acceleration and braking can be selected when centrifuging delicate


samples. The gentle settings avoid mixing of density gradients or breakup of
pellets.

This key controls rotor acceleration up to 400 RPM. If the yellow light over
the rabbit is lit, then full acceleration is selected. If the yellow light over the
turtle is lit, then slow acceleration is selected. Slow acceleration takes from
15 to 35 seconds to achieve 400 RPM, depending on the rotor and its
contents.

This key controls rotor braking. If the yellow light over the rabbit is lit, full
braking is selected. If the yellow light over the turtle is lit, slow braking is
selected. (This means the rotor will coast down from 600 RPM.) If both
lights are out, all braking is disabled; the rotor will coast from operating speed
to a stop.

Centra MP4/MP4R Operation Manual 7


3 OPERATION

3.1 Rotor and Accessories


A balanced load is essential with all centrifuges. An unbalanced load
produces vibration and can damage the unit. A 2 gram load imbalance, at a
speed of 4600 RPM, imparts force equivalent to 9.1 kg at rest (20 pounds).
Therefore, always ensure that the rotor is loaded symmetrically with a full
complement of accessories, and a full (or paired) set of tubes. Tube
adapters should also be installed symmetrically.

IEC rotors are dynamically balanced at the factory. IEC matches removable
parts (trunnion rings, shields, buckets and carriers) to within 1 gram and
stamps the weight on each piece. Check these markings whenever you
interchange parts, to ensure that opposite parts are matched. Ensure that
the total weight of samples and removable parts loaded in opposing positions
are equal in weight to within 1 gram. The position numbers, present on many
rotors and adapters, identify opposing tube positions.

To obtain good dynamic balance, the opposite loads must not only be equal
in mass, but must also have the same center of gravity. Opposing containers
must be alike in shape, thickness, and distribution of glass or plastic. This is
especially important for large containers.

Tubes loaded into swinging bucket rotors must be symmetric around the axis
of rotation. Verify this by rotating the entire rotor 180° by hand: the loads
should be in the same apparent positions (not in the mirror image). In
addition, the loads within each bucket must also be symmetric around the
bucket’s pivot axis. Verify this by ensuring that each bucket is loaded so that
it does not tilt from the vertical when the rotor is at rest. Maintaining balance
within each bucket ensures that the bucket and the tubes swing out to
horizontal when the rotor reaches operating speed, applying centrifugal force
toward the bottom of the tubes. Failure to achieve full swing-out causes
vibration and premature wear of both the rotor and the motor.

Samples of like (similar) specific gravities may be processed in the same run
provided the samples of the same type are balanced around the rotor as
though they were the only pairs in the rotor.

Caution: DO NOT exceed maximum rated speed for each rotor/


accessory combination. Maximum rated speeds can be
found in Section 4.1 Speed And Force Tables.

8 Centra MP4/MP4R Operation Manual


Rotor Balance For example, load tubes in the
following manner:

1. Load four tubes.


Positions 3, 6, 10, 13
or 2,5,9,12
or 1,4,8,11

2. Load six tubes.


Positions 6,7,3,13,14,10
or 5,7,2,12,14,9
or 1,7,4,8,14,11

3. Load an odd number of


tubes. Not recommended
(unless a dummy tube is used
for balance.)

Vibration All centrifuges have critical speeds at which vibration occurs. As the speed
increases beyond the critical speed vibration will cease. This inherent
condition also occurs during deceleration. An imbalanced load intensifies
these critical vibrations. Do not operate this centrifuge continuously at
observed critical speeds.

Centra MP4/MP4R Operation Manual 9


Rotor Installation To install a rotor, line up the keyway (slot) in the rotor with the
key in the shaft. Lower the rotor straight onto the shaft. A
correctly aligned rotor will slide down far enough that the shaft
protrudes through the top of the rotor. Secure the locking nut
onto the shaft using the wrench provided.

Caution: Failure to securely tighten the rotor retaining


nut against the rotor WITH THE WRENCH PROVIDED will
result in the rotor coming off the shaft.

The 851, 852 and 853 rotors require an IEC Part No. 50036 hub
adapter. Use the wide rotor locking nut to secure the rotor to the
tapered hub adapter. For easiest use, leave the rotor and hub
adapter secured together when installing and removing. Use the
locking nut to secure the hub adapter to the shaft, and tighten
with a coin or tool.

Caution: Never remove the key from the shaft in order


to use a non-standard rotor; equipment failure can result.

10 Centra MP4/MP4R Operation Manual


Rotor Removal To remove a rotor, first remove any sample tubes, shields, buckets and
other accessories from the rotor. Unscrew and remove the locking nut. If the
rotor is difficult to remove, place the locking nut back on the shaft and finger
tighten it. Unscrew it one turn. Grasp the rotor with one hand and pull upward
on it while tapping the locking nut with the locking wrench. The opposing
force should free the rotor to be lifted from the shaft.

Adding Rotors As new rotors are released by IEC, the rotor number and radius can be
added to the rotor menu. To do so, press the hidden key located behind the
“C” in IEC. Prog x.x will appear. Press RPM arrows until "rotor Add"
appears. Press the file key and "Rotor" will appear next to the Rotor/Radius
display. Press an arrow key under the rotor display to select the new rotor
number. Press the file key. "Radius" appears. Use arrow keys to select
maximum radius. Press file key again. "RPM" will be illuminated. Use the
RPM arrow key to select the maximum RPM for the rotor. Push file again.
Unit beeps three times to acknowledge addition of rotor. Press "C" in IEC to
return to normal operation. Note: Up to five rotors may be added to memory.
When the rotor memory is full, the unit displays "FUL" under the rotor
symbol.

Deleting Rotors Push "C" in IEC. Use RPM arrows to scroll to "rotor Add." Use the
time arrows to select "dEL" instead of "Add." Press file key. "Rotor"
appears. Use rotor arrows to select rotor to be deleted. Press file key to
delete. Unit beeps three times to acknowledge deletion of rotor.

3.2 Starting and Stopping a Run


Read Section 2.5 for a general description of the front panel. The desired
settings displayed on the front panel always govern the operation of the unit.
The number or symbol displayed above the word PROGRAM describes the
operating mode of the unit. It is important that the unit be in the correct mode
for the desired operation.

The digits and symbols, which appear above the word PROGRAM when the
arrow keys are pressed, can be one of the following:

blank The unit is in manual operation.

0-9 The unit is under control of the stored program with the number
shown.

H The unit is set to hold mode, in which it runs until you stop it.

- The unit is set for a momentary spin.

C The unit is set to Rapid Condition, a special program discussed in


Section 3.4.

The display indicates the parameters last selected for each PROGRAM
mode of operation.

Centra MP4/MP4R Operation Manual 11


Manual Operation For manual operation, set the mode so that the display above the word
PROGRAM is blank. Select a desired temperature (MP4R only), speed/g-
force, run time, acceleration mode, and deceleration mode. To start the spin,
press the Start key. The spin stops automatically at the end of the desired
interval. A run can also be stopped manually by pressing the STOP key.

During a manual operation, the MINUTES display counts down and displays
the time remaining in the current spin.

The run time specified begins either when the START key is pressed, or
when the rotor reaches 95% of set speed (see TIMING MODE). When the
elapsed time reaches this desired setting, deceleration begins. That is, the
run time does not include deceleration time.

The settings can be changed during a manual run. These changes affect
the run in progress. If the time setting is changed during a run, the unit
adjusts the displayed count-down timer so that your revised setting will be
the total time of the run. If the new time selected is less than the elapsed
time, the run will end.

The unit’s mode (PROGRAM) cannot be changed during a spin.

Momentary Mode Momentary spin is useful for easily separated samples, for simultaneous
mixing of samples, and to deposit condensate droplets at the bottom of the
tube.

For momentary spin, set the mode so that a dash “-” appears above the word
PROGRAM. The dash also appears above MINUTES because there is no
programmed time in a momentary spin. Select temperature (MP4R only),
speed/g-force, acceleration mode, and deceleration mode, as for manual
operation.

Press and hold the START key. The run starts when you press the key and
ends when you release the key. In this mode you can perform very quick
separations or protocols.

During a momentary spin, the unit displays actual values, not desired
settings. The MINUTES display counts upward and displays the elapsed
time since you pressed the START key.

Hold Mode For hold mode (operation without time limit), set the mode so that H appears
above the word PROGRAM. The H also appears above MINUTES because
hold mode does not have a programmed time.

Select temperature (MP4R only), speed/g-force, acceleration mode, and


deceleration mode, as for manual operation. Press and release the START
key. The run starts when you press the key and stops only when you press
the STOP key. Hold mode is like manual operation, except that the time
setting is not used. During a run in hold mode, the MINUTES display counts
upward and displays the elapsed time of the spin. See Section 2.5 for more
information on the MINUTES display operation.

12 Centra MP4/MP4R Operation Manual


Timing Mode Two timing modes are available on these units:

ACC, for the set time to start counting down at the beginning of acceleration;
that is, when the start key is pressed. The unit is originally set to this mode.
SPd, for the set time to start counting down when the rotor has reached 95%
of set speed. Display will alternately display set time and SPd until counting
begins.

To select a timing mode, press the TIME down arrow key , and scroll below
zero. ACC or SPd will appear. Press and release the TIME down arrow key
to choose the desired mode. After three seconds, or after pressing the FILE
or START key, the display will return to the last selected run time provided
the up arrow key has not been pressed.

The timing mode is stored along with the other parameters when a program
is saved. If a saved program has been recalled, altered, and not re-saved,
the timing mode will revert back to the previous mode when recalled.

3.3 Stored Programs

The Centra-MP4 series has an internal memory capable of holding 10 sets of


run parameters. Each set, or program, is stored and can be recalled by
selecting a program number (0 - 9). Programs are retained in memory even
if the power is turned off. When necessary, a program can be modified for a
particular run or changed permanently. You cannot change the unit's
program, rotor/radius, or timing modes during a spin.

Locking Programs Programs can be locked from the program lock in the special function
menu. Press the "C" in IEC. Use RPM arrows to scroll until "Loc P" is
displayed. Use the program arrow keys to select the program to be locked.
Pressing the following keys in the following order will lock or unlock a
program: Start, Stop, Cover Open, Stop, Start and File (Save). The display
will alternate between the program number and an "L" indicating that the
values of the program are locked and cannot be changed.

Recall Program Press a program arrow key to select the appropriate program number.
The programmed run parameters will be displayed and will become the set
parameters. To begin this run, simply press START.

Add/Change Program Select a program number with the program arrow keys. The current
program parameters will appear on the display. Modify the desired
parameters using the parameter arrow keys, or the ACCEL or BRAKE
switches. To make the changes permanent, press the PROGRAM SAVE
(file folder) key. The program number will stop flashing, and the new program
will be displayed and will remain in memory until further changes are made.
To make changes temporary, press START without pressing the PROGRAM
SAVE (file folder) key. The program display goes blank to indicate that the
values are now stored in the manual program and the instrument is not
operating from the program mode at this time. As long as the PROGRAM
SAVE (file folder) key is not pressed, the original program remains
unchanged.

Centra MP4/MP4R Operation Manual 13


3.4 Centra MP4R Refrigeration
Whenever the cover is closed and the unit is switched ON, the unit applies
refrigeration to the rotor chamber as necessary to cool it to the currently
displayed temperature setting. However, using the keyboard so that a cold
temperature is momentarily displayed (for example, stepping through the
stored programs) does not activate refrigeration.

If a temperature higher than ambient is specified, the unit does not heat the
rotor chamber except through the normal heating effect of the equipment
(i.e. air friction and motor heat).

If the rotor chamber is not at the temperature specified, it does not abort the
spin. However, if the rotor chamber differs by 5°C or more from the specified
temperature at the start of a run, the unit sounds an audible alarm (see
Section 3.5). The °C display switches between the actual and programmed
temperature until the two temperatures come within 5°C. This shows the
reason for the alarm. Press the STOP key if the run should not continue at
the actual temperature.

The unit is not designed for use as a refrigerator. The natural fanning action
of the rotating horizontal and fixed angle rotors serves to maintain a uniform
temperature distribution inside the chamber. Therefore, at zero RPM, there
is no correlation between set and actual chamber temperatures.

Any frost or condensation that forms in the rotor chamber should be removed
by allowing it to melt and removing it with a sponge or cloth. When the
centrifuge is not in use, turn it off or leave the cover open (disabling
refrigeration).

Rapid Condition When the chamber temperature is above the set temperature, RAPID
(MP4R Only) CONDITION will run a rotor at 500 rpm to increase air circulation in the
chamber to quickly cool the chamber to the set point. When the chamber
temperature is below the set temperature, RAPID CONDITION will run the
rotor at 3400 rpm to warm the chamber to the set temperature. When the
temperature has been reached a three beep signal will sound and the rotor
will brake to rest. (Some smaller rotors may not be able to warm the
chamber to the higher temperature settings.) To select this program, press
the PROGRAM arrow keys until a 'C' appears in the PROGRAM display.
Select the desired temperature, install a rotor, and press the START key.

14 Centra MP4/MP4R Operation Manual


3.5 Warning Messages and Error Codes
The beeper sounds in these situations:

o Briefly whenever a key is pressed.

o Three times at the start and at the end of a spin.

o On the Centra-MP4R for 2 seconds if the START key is pressed and


the rotor chamber is more than 5° C warmer or cooler than your
desired temperature setting (see Section 3.4).

o Seven times when a warning occurs (see below).

Warning Messages appear in place of the numeric displays in the following


cases:

bAL This appears during a run if the rotor is unbalanced. The


rotor brakes to a stop and the run ends. The cover must be
opened to reset this warning. Verify that a balanced load is
installed. Inspect the rotor and rearrange tubes or add
additional, inactive tubes to balance the rotor.

HEAd This appears if you start a run without loading a rotor into the
chamber. The run ends. The cover must be opened to reset
this warning.

LId This appears briefly if you press the Start key but the cover
is not closed. Closing the cover resets the warning.

PFAIL This appears at the end of a run that is interrupted by a


power failure. Opening the cover resets this warning.

bruSh This appears at the end of every run if it is time to replace


the brushes. To reset the warning and replace the brushes,
see Section 5.4.

LInE LO/HI This appears as soon as power is applied to the unit if the
line voltage does not match the voltage configuration. It can
only be cleared by unplugging the unit and reconfiguring it
properly (see Section 2.3).

Warning Messages during a spin. The “bAL”, “LId”, and “PFAIL”


messages can occur during a spin. In this case, the rotor brakes to a stop
and the run ends.

Error Codes require factory-authorized maintenance. A typical error means


the internal microprocessor has detected impermissible readings or a failure
elsewhere in the unit. An error number appears on the front panel. Tell the
service personnel which number appeared when you report the problem.

Centra MP4/MP4R Operation Manual 15


Error 001: No Tachometer
Tachometer signals were not present during run. The rotor is
stopped under full brake. To open cover unplug and reinstall
the power cord.

Error 002: Overspeed


Speed is 500 RPM or more over set and rotor is not
decelerating, for more than one second or actual rotor speed
is over 14,500 RPM. The rotor will coast to a stop.

Error 003: Runaway


During stopping, rotor has not been decelerating for 8
seconds, or when at standby, rotor speed exceeds
600 RPM. The rotor coasts to a stop.

Error 004: Refrigeration Failure (Centra MP4R only)


The unit displays this code if the measured temperature
exceeds 45°C at any time during the run. The rotor will be
stopped under programmed deceleration.

Error 005: Fail-safe Time-out


Independent circuitry on the circuit board has sensed a lack
of activity from the control microprocessor. All power outputs
disabled (including motor, compressor, fans, latch solenoid,
etc.).

Error 006: COP Watchdog/Op-Code Trap


The microprocessor itself has sensed a lack of activity from
the program that controls the centrifuge. The rotor coasts to
a stop.

Error 007: Stack Error


The microprocessor itself has sensed a lack of activity from
the program that controls the centrifuge. The rotor coasts to
a stop.

Error 008: COP Watchdog Not Enabled


Microprocessor COP watch dog feature is not enabled.
The rotor coasts to a stop.

Error 009: Undefined Interrupt


Microprocessor was interrupted by an undefined source. The
rotor coasts to a stop.

16 Centra MP4/MP4R Operation Manual


4 APPLICATIONS

This section describes the use of specific rotors and accessories. More
detailed information is often shipped with the rotor or accessory itself. This
section contains five reference tables:

o Speed and Force Tables


o Derating Table for Dense Samples
o Chemical Resistance Table
o Decontamination Table
o Nomograph

Relative centrifugal force (RCF or G-force) at a given speed varies with the
rotor, and with the length of the sample tube, because the distance of the
tube’s tip from the center of rotation is different. The Speed and Force
Tables indicate the maximum speed and RCF the Centra MP4 and the
Centra MP4R can achieve with various rotor/accessory combinations. The
Derating Table specifies reductions in maximum RPM when spinning
samples with specific gravity above 1.2.

Misapplication of any tube can cause tube rupture. To avoid this, compare
the G forces specified in the Speed and Force Table with the ratings for the
tubes you are using. If the tubes are not rated for the force the centrifuge will
apply, reduce the speed to the G-force listed for your tubes.

Corrosive Solvents Your IEC centrifuge is made of materials designed to resist attack from most
laboratory chemicals. The interior of the rotor chamber is stainless steel.
Rotors and accessories placed in the chamber are made of a variety of
materials, including aluminum and polypropylene. The Chemical Resistance
Table shows the suitability of each material with different classes of reagents.

Section 5.1 describes how to clean and remove corrosion from the chamber,
rotors, and accessories. Follow these instructions, and clean spills promptly,
to minimize the effect of corrosive chemicals and to avoid expensive repairs.

Centra MP4/MP4R Operation Manual 17


4.1. Speed and Force Tables

ROTOR 215 4-Place Horizontal Swinging Bucket


Tube Trunnion/ Max Max
No. of Maximum O.D. x Length (mm)
Volume Shield or RCF Radius
Places Speed (rpm) Common Tube Size
(ml) Carrier (xg) (cm)
4 80 366/365 4600 3500 14.8 38x118

4 50 325/320** 4600 3300 14.0 30x116

4 50 350/323** 4300 3200 15.2 Falcon/Corning Conical

4 10 310/356 4900 3550 13.2 17.2x113

8 50 326/320 4100 2675 14.2 30x116

12 10 355/356 4100 2475 13.2 17.2x112


12 10 366/1013 4600 3025 12.8 16.5x107 16x75;100 Vacutainer

16 5-7 366/1018 4700 3150 12.8 14.2x103 13x75;100 Vacutainer

20 3-5 366/369 5200 3350 11.1 12.6x83 10;12x75

4 3 1024 2050 500 10.6 1"x3" Microscope Slide


** Order Adapter 1106 And 571 Cushion To Spin 15ml Falcon/Corning Conical Tubes

ROTOR 221 6-Place Horizontal Swinging Bucket


Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
6 10 356 5000 3625 13.0 17.2x102

6 15 303 4500 3300 14.6 17.2x119

18 Centra MP4/MP4R Operation Manual


ROTOR 224 Horizontal Swinging Bucket with Cat. No. 3224 Cups
Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm) Common Tube Size
4 250 ---- 4400 3310 15.3 IEC 2050, 2051

4 175 ---- 4400 3270 15.1 Falcon 2076 w/2090 cushion

4 80 7221 4400 3180 14.7 38.1x111

4 50 7231 4400 3180 14.7 Falcon/Corning Conical

12 30 7223 4400 3180 14.7 IEC 2047, 2055, 2802

16 15 7230 4400 3180 15.1 Falcon/Corning Conical


28 15 7224 4400 3180 14.7 16.2x100

28 10-15 7225 4400 3180 14.7 13;16x75;100 Vacutainer/HG


40 7 7236 4400 3180 14.7 13x100 Vacutainer

48 3-5 7226 4400 3180 14.7 12x75; 10x75

40 3 7228 4400 3180 14.7 10x50

40 1.5 7228 4400 2970 13.7 1.5 Microtube

ROTOR 244 2-Place (Tray) Horizontal Microplate


Max
No. of Tube Carrier Maximum
Max
Radius O.D. x Length (mm)
Places Volume Speed (rpm)
RCF
(ml) (xg) (cm)
2 ---- 49852 4,000* 1800 10.0 86x128 (96 well) Array Racks
*See Derating
*Speed LimitedTable For 244 Rotor

ROTOR 794 4-Place Fixed Horizontal


Tube Tray Max Max
No. of Maximum
Volume Insert RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
40 .25-.4 5914 11,000
12,800 8,120
11,000 4.7-6.0 6.0x46 Microtubes

40 1.5-2 5912 12,800


11,000 11,000
8,120 6.0 11.0x39 Microtubes

ROTOR 796 6-Place Fixed Horizontal


Tube Tray Max Max
No. of Maximum
Volume Insert RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
60 .25-.4 5914 9000 6350 5.7-7.0 6.0x46 Microtubes

60 1.5-2 5912 9000 6350 7.0 11.0x39 Microtubes

Centra MP4/MP4R Operation Manual 19


ROTOR 801 6-Place Fixed Angle 45°
Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
6 50 305 5200 3650 12.1 30x133 Falcon/Corning Conical*

6 50 320 6500 5100 10.8 30x116 Corning 8300-50

*With IEC 315 Conical Cushion

ROTOR 803 6-Place Fixed Angle 45°


Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
6 10 356 8300 7850 10.2 17.2x88

6 15 303 7400 6850 11.2 17.2x102

6 19 302 6300 5600 12.6 17.2x122

ROTOR 804S 4-Place Fixed Angle 45° with 323 Sealed Dome Shields
Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
4 50 323* 7200 6675 11.5 Falcon/Corning Conical

*Order Adapter 1106 And 571 Cushion To Spin 15 Ml Falcon/Corning Conical Tubes

ROTOR 809 12-Place Fixed Angle 45°


Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
12 10 356 7500 6425 10.2 17.2x88

12 15 303 6500 5300 11.2 17.2x102

12 19 302 5500 4250 12.6 17.2x122

20 Centra MP4/MP4R Operation Manual


ROTOR 815 24-Place Fixed Angle 33°
Tube Max Max
No. of Shield Maximum O.D. x Length (mm)
Volume RCF Radius
Places Speed (rpm)
(ml) (xg) (cm)
24 10 356 6500 5250/4300 11.1/9.1 17.2x88

24 15 303 5500 4050/3375 12/10 17.2x102

24 19 302 5000 3625/3075 13/11 17.2x122 Falcon/Corning Conical

ROTOR 816 8-Place Fixed Angle 33°


Tube Max Max
No. of Shield Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
8 50 320 6300 5200 11.7 30x115 Corning 8300-50

8 50 305 5600 4500 12.8 30x134 Falcon/Corning Conical*

*With IEC 315 Conical Cushion

ROTOR 818 12-Place Fixed Angle 35°


Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Speed (rpm)
(ml) (xg) (cm)
12 5 ---- 13,400 15,050 7.5 12x80 IEC 2804, 2840

12 1.5-2 5762 13,400 11,050 5.5 11x66

12 B-D/.5 5761 13,400 11850/10050 5.9/5.0 8x66

12 .4/.25 5760 13,400 11850/10050 5.9/5.0 6x66

ROTOR 819 10-Place Fixed Angle 35°


Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
10 10 ---- 13,400 15,250 7.6 16.1x85IEC2046,2067,2801,2850

10 5 5986 13,400 14,850 7.4 12.1x85

10 4 5987 13,400 14,650 7.3 10.9x90IEC2804,2840,2841,2852


10 1 5989 13,400 14,450 7.2 6.1x52 Corning 9820-6

Centra MP4/MP4R Operation Manual 21


ROTOR 836 6-Place Fixed Angle 30°
Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
6 30 ---- 13,400 15,050 7.5 25.8x100 IEC 2047,2055,2802
6 15 5965 13,400 14,450 7.2 16.1x109
6 5 5966 13,400 14,050 7.0 12.1x112
6 3 5967 13,400 13,650 6.8 10.9x95

ROTOR 841 12-Place Fixed Angle 45°


Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
12 1.5-2 ---- 14,000 12,700 5.8 11x39
12 B-D 5763 14,000 12,925 5.9 B-D Microtainer Tubes
12 .5 5763 14,000 10,750 4.9 8x66
12 .4 5764 14,000 12,700 5.8 6x46
12 .25 5764 14,000 9850 4.5 6x46

22 Centra MP4/MP4R Operation Manual


ROTOR 851(651)++ 24-Place Fixed Angle 45°
Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
24 1.5 ---- 13,200 16,250 8.35 11x45

24 B-D 5763 13,200 16,450 8.45 B-D Microtainer Tubes

24 .5 5763 13,200 14,500 7.45 8x32

24 .4 5764 13,200 16,250 8.35 6x50

24 .25 5764 13,200 14,100 7.25 6x32


++50036 Hub Adapter Required

ROTOR 852(652)++ 48-Place Fixed Angle 45°


Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
15,575* 8.0*
48 .5 ---- 13,200 8x32
14,600** 7.5**

24 B-D 5763 13,200 16,750* 8.6* B-D Microtainer Tubes

*Outer Row **Inner Row ++50036 Hub Adapter Required

ROTOR 853(653)++ 40-Place Fixed Angle 45°


Tube Max Max
No. of Adapter Maximum
Volume RCF Radius O.D. x Length (mm)
Places Number Speed (rpm)
(ml) (xg) (cm)
40 .4 ---- 13,200 16,350 8.4 6x50
40 .25 ---- 13,200 14,600 7.5 6x32

40 .8 ---- 13,200 16,500 8.5 6x50


++50036 Hub Adapter Required

Centra MP4/MP4R Operation Manual 23


ROTOR 854 4-Place Fixed Angle 20°
Max Max
No. of Tube Adapter Maximum
Radius O.D. x Length (mm)
RCF
Places Volume Number Speed (rpm)
(cm)
(ml) (xg)

4 50 ---- 12,900 14,000 7.53 28.4x106 IEC1630,2048,2053,2997

4 30 7243 12,900 13,400 7.2 25.4x93 IEC 2047,2055,2802

4 30 Corning 8445-AO 12,200 12,100 7.2 Corning 8445-30

4 25 Corning 8446-AO 12,200 12,100 7.2 Corning 8446-25

24 Centra MP4/MP4R Operation Manual


4.2 Derating Tables

Dense Samples The Speed and Force Table lists the maximum speed for each rotor/adapter
combination in the Centra-MP4/R. These speeds are guaranteed only with
samples whose specific gravity is not greater than:
1.2 for swinging bucket rotors
1.5 for fixed angle rotors
For denser samples, the maximum guaranteed speed is reduced (derated)
by a factor from the table below:

Derating Factor for:

Specific Gravity Swinging Bucket Fixed Angle


1.2 1. 1.
1.3 .960 1.
1.4 .925 1.
1.5 .894 1.
1.6 .866 .967
1.7 .839 .939
1.8 .816 .912
1.9 .794 .888
2.0 .774 .866
2.1 .755 .844
2.2 .738 .825
2.3 .721 .807
2.4 .707 .790
2.5 .692 .774
2.6 .678 .758
2.7 .666 .744
2.8 .654 .731
2.9 .642 .719
3.0 .632 .707

Example. An angle rotor rated for 10,000 RPM, used with samples with a
specific gravity of 1.6, cannot spin faster than (10,000 x .967 =) 9,670 RPM.

Specific gravities greater than 3.0. This table is based on the formula:
√ (s0/sa)
...where s0 is the maximum specific gravity allowed before derating (1.2 or 1.5,
depending on the type of rotor), and sa is the actual specific gravity of the
sample in question. You can use the same formula to compute derating
factors for specific gravities greater than 3.0.

Caution: Do not exceed the rated speed. Higher speeds will


impose unnecessary wear on the centrifuge and can
cause ROTOR FAILURE.

244 Rotor The 244 rotor is designed for centrifugation of multi-well microplates.
The weight of the loaded microplates must be equally distributed between
the rotor's two carriers. The maximum rated speed for the 244 rotor when
each of the carriers is loaded with 280 grams (total load of 560 grams) is
4000 rpm in the MP4/R.

Centra MP4/MP4R Operation Manual 25


Caution: For loads greater than 280 grams (weight of microplate
and sample) per carrier, maximum speed must be
DERATED (according to the following table) to avoid
ROTOR FAILURE.

Derating Table for 244 Rotor

Load per carrier Max. Speed


280 grams 4000 rpm
300 grams 3600 rpm
320 grams 3300 rpm
340 grams 3000 rpm
360 grams 2800 rpm
380 grams 2600 rpm
400 grams 2400 rpm
450 grams 1900 rpm
500 grams 1400 rpm
600 grams 800 rpm
Greater than 600 grams Do Not Use

26 Centra MP4/MP4R Operation Manual


4.3 Chemical Resistance Table

Plastic Metal Other


PA PC PE PP PU NL DN CN NN PS TI SS AL MB MG RR BN VN PF
Acids, dilute or weak E E E E G E F N F E G G F F N F E E E
Acids*, strong or conc. E N E E F N N N N F N N N N N N F G N
Alcohols, aliphatic E G E E F E E E N E E E E E F E E G E
Aldehydes G F G G G G G G F N E E E E E E N E E
Bases E N E E N G N G F E E E E E E G G N N
Esters G N G G N E G G E N E E E E E N N N E
Hydrocarbons, aliphatic G F G G E N E E E N E E E E E N E E E
Hydrocarbons, aromatic F N G F N N E E E N E E E E E N N E E
Hydrocarbons, halogenated F N F F N N G E G N E E E E N N N F E
Ketones G N G G N N E E E N E G G G E N N N E
Oxidizing Agents, strong F N F F N N N N N N E F N N N N F E E
Salts E E E E E E E E E E E F F F N E E E E
* For Oxidizing Acids, see "Oxidizing Agents, strong".

PA - POLYALLOMER TI - TITANIUM
PC - POLYCARBONATE SS - STAINLESS STEEL
PE - POLYETHYLENE AL - ALUMINUM
PP - POLYPROPYLENE MB - MANGANESE BRONZE
PU - POLYURETHANE MG - MAGNESIUM
NL - MODIFIED PHENYLENE OXIDE (NORYL) RR - RUBBER
DN - ACETAL HOMOPOLYMER (DELRIN) BN - BUNA-N
CN - ACETAL COPOLYMER (CELCON) VN - VITON
NN - NYLON PF - PHENOLIC FIBER
PS - POLYSTYRENE

Classification of Resistance
E=Excellent
G=Good
F=Fair
N=Not Recommended

Centra MP4/MP4R Operation Manual 27


4.4 Decontamination Table

Compatible Processes For Decontamination


Sterilization Methods Plastic Metal Other
PA PC PE PP PU NL DN CN NN PS TI SS AL MB MGRR BN VN PF PT
Mechanical
Autoclave* S M U S M U S S S U S S S S S S S M S M
Ethylene Oxide Gas S S S S S S S S S S S S S S S U U S S S
Dry Heat 160°C@2Hrs. U U U U U U U U U U S S U S S U U U U U
Chemical
Ethanol S S S S U S S S U M S S S S S S S S S S
40% Formalin S S S S U S S S S U S S S S S S U S S S
Methanol S M S S M S S S U M S S S S S S S U S S
2-Propanol S S S S M S S S U S S S S S M S S S S S
5% Sodium Hypochlorite** S S S S U S U U U S S M U U U S U S S M
3% Hydrogen Peroxide S S S S S S M S U S S S S S U S S S S M
100% Hydrogen Peroxide S S S S S U U U U S S S S S S U U S S U
5% Phenol Solution M U U S U U M M U M M M M M M M U S S U

PA - POLYALLOMER TI - TITANIUM *Autoclaving


PC - POLYCARBONATE SS - STAINLESS STEEL 121°C 20 min.@
PE - POLYETHYLENE AL - ALUMINUM 2 ATM (15 PSIG)
PP - POLYPROPYLENE MB - MANGANESE BRONZE
PU - POLYURETHANE MG - MAGNESIUM
NL - MODIFIED PHENYLENE OXIDE (NORYL)
RR - RUBBER **Household Bleach
DN - ACETAL HOMOPOLYMER (DELRIN) BN - BUNA-N
CN - ACETAL COPOLYMER (CELCON) VN - VITON S=SATISFACTORY
NN - NYLON PF - PHENOLIC FIBER M=MARGINAL
PS - POLYSTYRENE PT - PAINTED SURFACES U=UNSATISFACTORY

WARNING:
This chart describes the material compatibility of various sterilization methods. It does not specify the
adequacy of sterilization. Refer to section 4.3 Chemical Resistance Table for material compatibility
during centrifugation.

28 Centra MP4/MP4R Operation Manual


4.5 RCF Nomograph

Centra-MP4/MP4R Operation Manual 29


5 MAINTENANCE

5.1 Cleaning
Keep your centrifuge clean to ensure good operation and to extend its life.
Clean the entire sample chamber, rotor, and lid at the end of each workday,
and also right after any spill.

To clean the sample chamber, use a damp sponge, warm water, and a mild
liquid detergent suitable for washing dishes by hand, such as Ivory® liquid.
Do not use caustic detergents or detergents that contain chlorine ions, since
these attack metals. Remove stubborn stains with a plastic scrub pad. Do
not use steel wool, wire brushes, abrasives, or sandpaper, since they create
corrosion sites. Never pour water directly into the sample chamber. Scrub
the rotor’s tube cavities with a stiff test-tube brush that has end bristles and a
non-metallic tip. After cleaning any part, dry it properly, preferably using a
clean, absorbent towel.

If glass breakage occurs, remove all broken pieces immediately. Glass


particles, if present in the chamber, will be ground into a fine grey dust during
centrifugation. If glass breakage recurs it is recommended that all adapters
and cushions be replaced. Particles of broken glass become imbedded in
the plastic or rubber accessories. These particles can come in contact with
new glass tubes, creating a pressure point which may result in recurring
glass breakage.

Cleaning swinging bucket rotors is necessary to ensure that the buckets


can pivot freely. Periodically manipulate each bucket; if you feel resistance
or hear squeaking, lubricate all buckets with Bonded Lubricant Coating
(BLC), IEC Part No. 7133. Use the following cleaning and lubrication
procedure:

1. Wipe the old lubricant from all rotor pins and buckets with a soft, clean,
lint-free cloth saturated with solvent such as trichloroethylene.

2. Clean the rotor and buckets as described above. The cleaning step is
important because BLC only adheres to a clean surface. If you are
unable to remove foreign matter in this way, contact an authorized IEC
Service Representative.

3. Shake the bottle of BLC vigorously until all the gray sediment at the
bottom of the bottle is dispersed.

30 Centra MP4/MP4R Operation Manual


4. Use the brush applicator cap to apply a light coating of BLC to the bucket
slots only. Do not lubricate the pins. Lubricant will move around the pins
during a spin.

5. Give the BLC 1 to 2 minutes to dry. Buff the bucket slots vigorously with
a soft, clean, lint-free cloth. Continue until no more BLC rubs off onto the
cloth. The surface will be a shiny, light gray.

Corrosion IEC manufactures and finishes rotors and structural accessories to give
maximum resistance to corrosion. However, maximum equipment life
requires that you continually inspect the rotor cavities for corrosion,
especially after using chloride ion solutions, such as sodium chloride (saline),
and sodium hypochlorite (household bleach). These solutions attack most
metals. Clean the rotor, rotor chamber, and accessories (particularly the
sample compartments and bucket cups) thoroughly after each such use.
Inspect all surfaces under bright light for corrosion; small crevices will grow
deeper and cause failure.

Replace the shaft, metal locking screw, rotor or accessories if they become
badly corroded, cracked deformed or gouged.

If you see any corrosion, remove it immediately as follows:

1. Follow the cleaning procedure at the start of this section. Soak the part
in the mild hand-dishwashing detergent. Scrub the part thoroughly with a
stiff test-tube brush having end bristles and a non-metallic tip.

2. Soak the part again in clear warm water for at least an hour.

3. Rinse the part thoroughly in warm water first, then in distilled water.

4. Dry the part thoroughly with a clean, absorbent cloth.

5. If this procedure does not remove the corrosion, discontinue use of the
part.

Storage Store parts on a soft surface to avoid damaging finished surfaces. Rotors
and other parts should be clean and dry for storage. Store them open to the
atmosphere, not in a plastic bag, so that any residual moisture will evaporate.
The parts should face downward to avoid retaining moisture in the cavities.

Decontamination Decontamination is called for if tube breakage occurs and infectious,


pathogenic, or radioactive material is released into the unit. Some rotors
totally contain the sample tubes. In this case, spillage is usually confined to
the rotor. If so, it may be sufficient to decontaminate the rotor. The
Decontamination Table lists the sensitivity of various materials to common
sterilization procedures. When using a 1-to-10 dilution of household bleach
(sodium hypochlorite) to decontaminate metal rotors or accessories, follow
decontamination by the corrosion cleaning procedure given earlier, since
chloride ions attack most metals.

Centra MP4/MP4R Operation Manual 31


Always decontaminate for the minimum recommended time. If you observe
corrosion, remove it as described earlier, discontinue use of the method, and
use an alternate decontamination procedure.

Sterilization of polypropylene rotors can be done by autoclaving. Remove


any sample tubes before autoclaving, unless they are completely full of
sample, or remove caps, stoppers, and other tube closures, before
autoclaving to keep the tubes from collapsing under pressure. Autoclave the

rotor and accessories at 121° C @ 15 psig for 20 minutes. Do not stack


polypropylene rotors during autoclaving. After the rotor is cool to the touch,
do a normal cleaning operation as described above.

Repeated autoclaving will seriously degrade the performance of


polycarbonate materials.

5.2 Cover Interlock Bypass


If power fails, the cover remains locked. If you need to remove samples from
the unit before power is restored, use the cover interlock bypass after the
rotor has come to a stop.

Unplug the centrifuge. Locate a hidden plug just below the front panel. Use
a screwdriver to remove this plug. Pull the attached cord to release the cover
interlock. Reassemble the plug in the hole.

Do not perform this operation routinely. The centrifuge’s cover interlock


provides operator safety and allows the cover to be opened promptly
whenever rotation has stopped.

5.3 Calibration

The built-in, independent digital tachometer in your centrifuge is calibrated by


IEC according to standards that are traceable to the U.S. National Institute of
Standards and Technology (NIST). The built-in tachometer uses crystal
standards that do not drift. Therefore, IEC recommends verifying the RPM
indicator once every 24 months. This can be done easily using an optical
tachometer through the clear plastic viewport in the lid. If this measurement
indicates instrument tachometer failure, please notify IEC Technical Service.

32 Centra MP4/MP4R Operation Manual


5.4 Brush Replacement
Brush replacement is required when brush length is 1/4 inch or less, or when
the front panel displays “bruSh” at the end of a spin. The "bruSh" warning
occurs every 700 hours of actual spin time. A set of replacement brushes is
included with the centrifuge.

Open the cover and unplug the centrifuge. Unscrew the locking screw and
remove the rotor and all accessories. Locate the rubber motor boot at the
bottom of the rotor chamber. It is attached to the motor by a ring with 4
screws, and is fitted to the rotor chamber. Gently push the boot toward the
motor shaft to seperate it from the guard bowl. Locate and unscrew the two
brush holder caps (protruding from both sides of the motor). Take care not
to drop the brush caps into the motor well! Inside each cap is a brush
assembly, consisting of a rectangular carbon brush, a spring, and a copper
wire inside the spring. Remove and discard from both sides of the motor the
entire assembly inside each cap. Replace them with the spares. The
brush’s rectangular shape keeps it from being inserted improperly. Screw the
caps back in. Reinstall the motor boot assembly. Plug the centrifuge back
into the power outlet.

Order a new set of brushes; IEC Part No. 50433.

The “bruSh” warning indication on the front panel must now be reset. To do
so, follow this procedure:

1. Press the SPECIAL FUNCTIONS button located behind the “C” in IEC.
The RPM display now shows one of a set of Special Functions. This
menu is primarily used by service personnel.

2. Press either RPM arrow key until the RPM display shows “bruSh”. The
MINUTES display now shows the hours of brush life. At brush
replacement, this number is 700 or over.

3. Press the File key to set the brush life back to 0, reflecting the installation
of new brushes.

4. Press the SPECIAL FUNCTIONS button again to return the unit to


normal operation.

Centra MP4/MP4R Operation Manual 33


5.5 Table of Spare Parts

48407 Rotor Locking Screw


9946 Fuse 6.3 A, 200/240 V
50058 Fuse 16 A, 100/120 V
50433 Brushes, One Pair
50431 Brush Caps, One Pair
7133 BLC (Bonded Lubricant Coating)

5.6 Warranty

IEC wants you to be satisfied with the quality of your Centra-MP4R or


Centra-MP4 centrifuge. We warrantee your IEC centrifuge for one year and
IEC rotors for seven years. We will repair or replace any of these products
that fails, within this period from the date of its delivery, due to defects in
material and workmanship, and we will ship you the repaired product or its
replacement at our expense. You must use IEC approved accessories and
genuine IEC spare parts. This warranty does not apply to any instrument
that has been abused or repaired without authorization.

THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER


OBLIGATIONS AND LIABILITIES INCLUDING NEGLIGENCE, AND ALL
WARRANTIES, OF MERCHANTABILITY OR OTHERWISE, EXPRESSED
OR IMPLIED IN FACT OR BY LAW. THE FOREGOING STATES OUR
ENTIRE AND EXCLUSIVE LIABILITY, AND BUYER’S EXCLUSIVE
REMEDY, FOR ANY CLAIM OR DAMAGES IN CONNECTION WITH THE
SALE OR FURNISHING OF GOODS OR PARTS, THEIR DESIGN,
SUITABILITY FOR USE, INSTALLATION, OR OPERATION. IEC WILL IN
NO EVENT BE LIABLE FOR ANY SPECIAL OR CONSEQUENTIAL
DAMAGES WHATSOEVER, AND OUR LIABILITY UNDER NO
CIRCUMSTANCES WILL EXCEED THE PURCHASE PRICE FOR THE
GOODS FOR WHICH LIABILITY IS CLAIMED.

34 Centra MP4/MP4R Operation Manual


5.7 Condition of Returned Equipment

Before returning equipment to IEC, you must contact IEC or your dealer and
receive a return goods authorization (RGA). All returned units must be
decontaminated, free of radioactivity, and free of hazardous and
infectious materials. The RGA paperwork includes a Certificate of
Decontamination for you to sign indicating that you have performed these
steps. IEC will not accept the shipment until this signed certificate is
received.

You must prepay transportation to the service depot.

5.8 Fuses Not Replaceable By The Operator

Four internal fuses are not replaceable by the operator. These


fuses should only be replaced by qualified service personnel.

F1 2.5A T 250V
F2 6.3A T 250V
F3 2.0A T 125V
F4 1.0A T 125V

Centra MP4/MP4R Operation Manual 35


6 SPECIFICATIONS

Maximum Speed: 14,000 RPM (817 fixed angle rotor)


4,400 RPM (224 horizontal rotor)
Maximum Force: 16,750 xg (852 fixed angle rotor)
Maximum Number of Tubes: 60 x 1.5 ml (796 rotor)
Maximum Sample Volume: 1 liter (224 rotor)

Operator Controls:
Chamber Temp.*: -5° C to 40° C, by 1 C°
Rotation: 300 -14,000 RPM, by 100 RPM
1 - 100 xg, by 1 xg
101 xg and up, by 10 xg
Spin Duration: 0:01 through 0:59, by 1 second
1:00 through 4:45, by 15 seconds
5 through 360 min., by 1 min.
Momentary operation mode
Hold mode (indefinite duration)
Timing mode

Repeatability:
Temperature Control*: +/- 1 C° in the range from +4° C to ambient
Rotation to 10 RPM
Spin Timing 0.1 seconds

Motor: Series-wound universal motor


Brake: Automatic dynamic electric brake
Refrigeration System*: Hermetic compressor (1/4 hp @ 60 Hz)
Refrigerant*: HP-80, (5.8 oz.)
Fuses: 2x 16 A Slo-Blo (for 100 V/120 V)
2x 6.3A Slo-Blo (for 220V/240V)

Power Requirements
Voltage: User-configurable for:
100 V nominal ( 90-110 V)
120 V nominal (108-132 V)
220 V nominal (198-242 V)
240 V nominal (216-264 V)

Frequency: 50 Hz, 60 Hz
Current: 12 A Maximum (100 V/120 V)
6.5 A Maximum (220 V/240 V)

Heat Output: 950 BTU/hour typical (MP4R)


700 BTU/hour typical (MP4)

* MP4R Only

36 Centra MP4/MP4R Operation Manual


Dimensions (MP4R)
Height: 85 cm (33.5 in) (with cover open)
40 cm (15.5 in) (with cover closed)
Width: 47 cm (18.5 in)
Depth: 69 cm (27 in)
Weight: 95 kg (210 lbs) (shipping weight)
73 kg (160 lbs) (net weight)
Dimensions (MP4)
Height: 85 cm (33.5 in) (with cover open)
40 cm (15.5 in) (with cover closed)
Width: 47 cm (18.5 in)
Depth: 53 cm (20.75 in)
Weight: 73 kg (160 lbs) (shipping weight)
50 kg (110 lbs) (net weight)

Sound Level: 65 dba

Specifications Subject To Change Without Notice

Centra MP4/MP4R Operation Manual 37

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