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Lab Experiment 3

This document outlines an activity on monohybrid crosses involving yellow and white sampaguita flowers. Students will use Punnett squares to determine: 1) the F1 generation ratios from crossing a homozygous recessive yellow flower with a homozygous dominant white flower; 2) the F2 generation ratios from crossing the heterozygous F1 offspring; and 3) experimental results and ratios from conducting online simulations of crosses between green and gray phenotypes. Students are asked questions about null hypotheses, degrees of freedom, phenotype frequencies, and determining genotypes.

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John Eric Catalan
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78 views5 pages

Lab Experiment 3

This document outlines an activity on monohybrid crosses involving yellow and white sampaguita flowers. Students will use Punnett squares to determine: 1) the F1 generation ratios from crossing a homozygous recessive yellow flower with a homozygous dominant white flower; 2) the F2 generation ratios from crossing the heterozygous F1 offspring; and 3) experimental results and ratios from conducting online simulations of crosses between green and gray phenotypes. Students are asked questions about null hypotheses, degrees of freedom, phenotype frequencies, and determining genotypes.

Uploaded by

John Eric Catalan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Activity #3

Monohybrid Cross
I. Objectives
The students should be able:
a. Determine the mechanism by which a single pair of genes of
contrasting trait combine

II. Learning Platform


a. MS Teams
b. Lab Activity Worksheet

III. Materials
a. laptop or desktop computer with internet connection drawing
materials: pencil, eraser, gel pen, coloring pencils/crayons
b. Lab Experiment 3 Worksheet
c. Online Lab Simulation:
a. https://exchange.iseesystems.com/public/jondarkow/si
mple-genetics/index.html#page1

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IV. Lab Report Activity
a. Part 1: F1 Generation
Yellow Sampaguita – Homozygous recessive (cc)
White Sampaguita – Homozygous dominant (CC)
a. With the given sampaguita flowers, you will be crossing
these two samples and identify the offsprings using a
punnett square. Indicate the ratio between each
offspring.

b. Part 2: F2 Generation
a. With the results you have from Part 1 and using a
Punnett square, what are the offsprings of your
heterozygous parents to identify the F2 generation.
Indicate the ratio between each offspring

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c. Part 3: Online Lab Simulation
a. Open the online lab simulation link:
https://exchange.iseesystems.com/public/jondarkow/si
mple-genetics/index.html#page1
b. Conduct 5 trial runs for the genetic cross between
i. Mother Phenotype – Green & Father Phenotype –
Gray
ii. Mother Phenotype – Gray & Father Phenotype –
Green

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c. Insert your data in the table below
i. Mother Phenotype – Green & Father Phenotype –
Gray

Trials Green Offspring Gray Offspring


1
2
3
4
5
Total Observed Results

ii. Mother Phenotype – Gray & Father Phenotype –


Green

Trials Green Offspring Gray Offspring


1
2
3
4
5
Total Observed Results

d. Based on your results, which of the parents do you think


is carrying the dominant phenotype? Why?

V. Conclusion
a. Write your conclusion

VI. Appendix
Proof of conducting the laboratory activity

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VII. Post-Lab Activity
I. Answer the following questions.
a. What is a null hypothesis in experiments? How is a null
hypothesis used in science experiments?
b. In a coin toss, how many degrees of freedom are there?
In a dice roll, how many degrees of freedom are there?
c. What determines how often a phenotype occurs in a
population?
d. Are dominant characteristics always more frequent in a
population than recessive characteristics? Why or why
not?
e. Is it possible to determine the genotype of an individual
having a dominant phenotype? How?

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