Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.

2016
NO Macro Version run no:

Ct-
Well Sample Replica Out Ct dRN copies DCt
average
A1 Lectin- replic1 19,13 0,43 86.400,0 19,11 Module
A2 standard 1 replic2 19,09 0,43 System
A3 Lectin- replic1 21,77 0,44 14.400,0 21,74 2,58 Platelayout
A4 standard 2 replic2 21,70 0,44 2,63

A5 Lectin- replic1 24,45 0,42 2.400,0 24,39 2,58 Instrument

A6 standard 3 replic2 24,32 0,43 2,65 Run ID
A7 Lectin- replic1 26,89 0,43 400,0 26,98 2,58 Reference DNA
A8 standard 4 replic2 27,07 0,43 2,60 Lot 1,0% Reference
D1 35S- replic1 22,36 0,51 8.640,0 22,32 DNA source
D2 standard 1 replic2 22,27 0,53 Set value
D3 35S- replic1 24,92 0,53 1.440,0 24,78 2,58 Zygosity
D4 standard 2 replic2 24,64 0,54 2,47 Standard-DNA
D5 35S- replic1 27,53 0,51 240,0 27,50 2,58 Type
D6 standard 3 replic2 27,46 0,54 2,72 Zygosity
D7 35S- replic1 29,21 0,54 40,0 29,52 2,58

D8 standard 4 replic2 29,82 0,54 2,02

calibration curve Lectin calibration curve 35S

38 slope -1,466 38
Lectin
-3,375
ct-average

intersect 35,773

ct-average
efficiency 97,8%
28 R² 1,0000 28

threshold ≥0,98

18 18
10 100 molecules 1000 10000 100000 10

A9 NTC replic1 -- 0,02

A10 Lectin replic2 -- 0,01
D9 NTC replic1 -- 0,02
D10 35S replic2 -- 0,02
average
Well Sample Replica Out Ct dRN copies % 35S LOQ
35S
A11 Lectin a) 21,37 0,43 18.529 0,47%
A12 1,00% Lectin b) 21,39 0,44 18.278 0,94% 0,70% 0,05%
D11 35S a) 28,62 0,53 87
D12 35S b) 27,70 0,54 171
B1 Lectin a) 19,15 0,42 84.275 1,17% result
B2 0.9 Lectin b) 18,97 0,44 95.288 1,05% 1,11% 0,01% 1,13%
E1 0.9 gmo 35S a) 25,33 0,51 982
E2 35S b) 25,30 0,53 1.004
B3 Lectin a) 21,09 0,44 22.430 1,06%
B4 0.9 dil Lectin b) 21,03 0,45 23.367 1,23% 1,15% 0,04%
E3 0.9 dil 35S a) 27,25 0,53 239
E4 35S b) 27,00 0,55 287
B7 Lectin a) 21,58 0,43 16.056 75,47% result
B8 Soy dil Lectin b) 21,72 0,43 14.593 73,80% 74,64% 0,07% 66,05%
E7 Soy dil 35S a) 21,92 0,55 12.117
E8 35S b) 22,08 0,54 10.770

'Evaluation 1/6
 Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.2016
NO Macro Version run no:

B5 Lectin a) 19,61 0,42 61.573 45,59%

B6 soia Lectin b) 19,89 0,43 50.865 69,35% 57,47% 0,02%
E5 35S a) 20,78 0,54 28.070
E6 35S b) 20,47 0,54 35.274
B9 Lectin a) 45,00 0,00 0 #DIV/0! result
B10 0,9% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
E9 1 35S a) 45,00 0,00 0
E10 35S b) 45,00 0,00 0
B11 Lectin a) 45,00 0,00 0 #DIV/0!
B12 0,9% - Dil Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
E11 5 35S a) 45,00 0,00 0
E12 35S b) 45,00 0,00 0
C1 Lectin a) 45,00 0,00 0 #DIV/0! result
C2 0,085% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
F1 1 35S a) 45,00 0,00 0
F2 35S b) 45,00 0,00 0
C3 Lectin a) 45,00 0,00 0 #DIV/0!
C4 0,085% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
F3 1 35S a) 45,00 0,00 0
F4 35S b) 45,00 0,00 0
c5 Lectin a) 45,00 0,00 0 #DIV/0! result
c6 0,05% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
F5 1 35S a) 45,00 0,00 0
F6 35S b) 45,00 0,00 0
C7 Lectin a) 45,00 0,00 0 #DIV/0!
C8 0,05% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
F7 1 35S a) 45,00 0,00 0
F8 35S b) 45,00 0,00 0
C9 Lectin a) 45,00 0,00 0 #DIV/0! result
C10 100% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
F9 1 35S a) 45,00 0,00 0
F10 35S b) 45,00 0,00 0
C11 Lectin a) 45,00 0,00 0 #DIV/0!
C12 100% Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
F11 1 35S a) 45,00 0,00 0
F12 35S b) 45,00 0,00 0
G1 Lectin a) 45,00 0,00 0 #DIV/0! result
G2 Sample 7 Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
G3 Enter Dil. factor 35S a) 45,00 0,00 0
G4 35S b) 45,00 0,00 0
G5 Lectin a) 45,00 0,00 0 #DIV/0!
G6 Sample 7 Dil Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
G7 Enter Dil. factor 35S a) 45,00 0,00 0
G8 35S b) 45,00 0,00 0

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 Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.2016
NO Macro Version run no:

G9 Lectin a) 45,00 0,00 0 #DIV/0! result

G10 Sample 8 Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
G11 Enter Dil. factor 35S a) 45,00 0,00 0
G12 35S b) 45,00 0,00 0
H1 Lectin a) 45,00 0,00 0 #DIV/0!
H2 Sample 8 Dil Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
H3 Enter Dil. factor 35S a) 45,00 0,00 0
H4 35S b) 45,00 0,00 0
H5 Lectin a) 45,00 0,00 0 #DIV/0! result
H6 Sample 9 Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0!
H7 Enter Dil. factor 35S a) 45,00 0,00 0
H8 35S b) 45,00 0,00 0
H9 Lectin a) 45,00 0,00 0 #DIV/0!
H10 Sample 9 Dil Lectin b) 45,00 0,00 0 #DIV/0! #DIV/0! #DIV/0!
H11 Enter Dil. factor 35S a) 45,00 0,00 0
H12 35S b) 45,00 0,00 0

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 Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.2016
NO Macro Version run no:

Cycles 45

GS-P-07.060 Lot
35S Soy Kit:
Platelayout Standard x
MasterMix:
Instrument RGQ x
25 10 2018 Standard:
Reference DNA x
Lot 1,0% Reference pentru validare
DNA source Kit
1,0%
homozygous
Standard-DNA Reporting
genomic HGE-Factor:
homozygous Reporting: %-DNA

bration curve 35S

slope -1,357
35S
-3,125
intersect 34,679
efficiency 108,9%
R² 0,9968
threshold ≥0,98

100 molecules 1000 10000 100000

DMolec.

StdDev
0,08% 1,17 x +30 %

3,8 N/A

4,1 N/A

4,1 N/A

3,5 N/A

StdDev
13,89%

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 Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.2016
NO Macro Version run no:

0,3 N/A

0,3 N/A

0,4 N/A

0,3 N/A

StdDev
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 Evaluationsheet quantitative RealTime PCR date of Evaluation: 10.02.2016
NO Macro Version run no:

StdDev
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