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    SOP Thermal Cycler

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    Bioteknologi Kelautan
    This document provides work instructions for using a thermal cycler. It describes how to turn on the instrument, start a run using a saved protocol, monitor a run, create and edit protocols, and turn off the instrument. Key steps include connecting the power supply, selecting and running a saved protocol, monitoring progress on the status or clock screen, and editing temperature, time and volume parameters for each protocol step.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    SOP Thermal Cycler

    Uploaded by

    Bioteknologi Kelautan
    260 views4 pages
    This document provides work instructions for using a thermal cycler. It describes how to turn on the instrument, start a run using a saved protocol, monitor a run, create and edit protocols, and turn off the instrument. Key steps include connecting the power supply, selecting and running a saved protocol, monitoring progress on the status or clock screen, and editing temperature, time and volume parameters for each protocol step.

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    SOP Thermal Cycler

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    This document provides work instructions for using a thermal cycler. It describes how to turn on the instrument, start a run using a saved protocol, monitor a run, create and edit protocols, and turn off the instrument. Key steps include connecting the power supply, selecting and running a saved protocol, monitoring progress on the status or clock screen, and editing temperature, time and volume parameters for each protocol step.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    260 views4 pages

    SOP Thermal Cycler

    Uploaded by

    Bioteknologi Kelautan
    This document provides work instructions for using a thermal cycler. It describes how to turn on the instrument, start a run using a saved protocol, monitor a run, create and edit protocols, and turn off the instrument. Key steps include connecting the power supply, selecting and running a saved protocol, monitoring progress on the status or clock screen, and editing temperature, time and volume parameters for each protocol step.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 4

    TROPICAL MARINE BIOTECHNOLOGY Document No.

    : 08
    LABORATORY
    WORK INSTRUCTON Effective Date :
    THERMAL CYCLER Review Date :

    Prepare by : Review by :

    Salwa Layalya, A.Md.Si Prof. Dr. Ir. Agus Sabdono, M.Sc


    Laboratory Technician Laboratory Coordinator
    Date : Date :

    Work Instruction

    A. TURNING ON THE INSTRUMENT


    1. Connect the cable power to a power supply. Turn ON the power switch on the
    back panel.
    2. The instrument runs a self-test to verify proper function, and then displays the
    home screen (figure 1).

    Figure 1. The home screen of the T100 thermal cycler.

    B. STARTING A RUN
    1. Open the lid, lift up the lid handle until the lid stays open.
    2. Put the samples into the block well.
    3. Close the lid, push the lid handle down until it stops.
    4. To run a saved protocol, touch Saved Protocols from the home screen (Figure 1).
    5. Choose the protocol (figure 2).

    Figure 2. The I SCRIPT protocol selected from the Saved Protocols screen

    6. Touch Run. The instrument confirms the run and the sample volume (Figure 3).

    Figure 3. The run confirmation window.

    7. Touch OK to run the protocol, or touch Volume to edit the sample volume.
    8. The Status screen opens (Figure 17). The run progress can be monitored in two
    views:
    • Status— a graphical view of the protocol and the current step in progress
    (Figure 4).
    • Clock— a large display of the time remaining for convenient monitoring
    (Figure 5).
    Figure 4. The Status screen.

    Figure 5. The Clock screen.

    C. CREATING A NEW PROTOCOL OR EDITING AN EXISTING


    PROTOCOL
    1. To create a new protocol, touch New Protocol from the home screen.
    2. To edit an existing protocol, touch Saved Protocols, select the protocol to be
    modified and touch Edit (figure 6).

    Figure 6. Select a protocol and touch Edit.


    3. The protocol editing screen opens (figure 7).

    Figure 7. The template for a PCR protocol.


    4. The protocol editor displays the protocol in a graphical format using editable
    boxes.
    5. To edit the temperature or time for each step, the sample volume, and the lid
    temperature, touch the appropriate box and enter a new value in the numeric
    keypad.
    6. Touch Run to run the protocol immediately without saving it. Alternatively,
    touch Save to choose a folder location and save the protocol, and then touch
    Run to run it.

    D. TURNING OFF THE INSTRUMENT


    1. Open the lid, take the samples from the block well.
    2. Close the lid, push the lid handle down until it stops.
    3. Turn OFF the power switch on the back panel.
    4. Unplug the cable power from a power supply.

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