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INSTRUCTIONS

Research High-Class Stereo


SZX10
Microscope

This instruction manual is for the Olympus SZX10 Research High-Class Stereo Microscope System. Optical Microscope and Accessory
To ensure the safety, obtain optimum performance and to familiarize yourself full with the use of this
microscope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
For details of products included in the configuration of this system, see “1 Nomenclature” (P. 4)
and “9 Operation Of Other Modules” (P. 23).

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SZX10
Contents

IMPORTANT........................................................................................................................................................ 1

1 NOMENCLATURE..................................................................................................................................... 4

2 CONTROLS.................................................................................................................................................... 5

3 SUMMARY OF OBSERVATION PROCEDURE................................................................... 6


3-1 Preparation............................................................................................................................................................... 6
3-2 Observation Procedure................................................................................................................................. 6

4 USING THE CONTROLS..................................................................................................................... 7


4-1 Base............................................................................................................................................................................... 7
1 Using the Stage Plate.................................................................................................................................................................7

2 Placing the Specimen................................................................................................................................................................7

4-2 Microscope Body and Focusing Assembly................................................................................ 7


1 Adjusting the Rotation Tension of the Coarse Focus Adjustment Knob......................................7

2 Engaging and Disengaging the Zooming Knob Click Stop Function............................................8

3 Adjusting the Aperture Iris Diaphragm........................................................................................................................8

4 Zoom Magnification Indication..........................................................................................................................................9

4-3 Observation Tube.............................................................................................................................................10


1 Adjusting the Interpupillary Distance......................................................................................................................... 10

2 Adjusting the Diopter (Zoom Parfocal Adjustment)...........................................................................................................................10

3 Using the Eye Shades............................................................................................................................................................ 11

4 Mounting the Eyepiece Micrometer Disk.............................................................................................................. 12

5 Selecting the Light Path of the Trinocular Tube..........................................................................................................................13

6 Adjusting the Tilt.......................................................................................................................................................................... 13

4-4 Camera Observation and Photomicrography.........................................................................14


1 Selecting the Camera Adapter Magnification................................................................................................... 14

2 Mounting the Camera Adapter....................................................................................................................................... 14

3 Selecting the Camera Camera Light Path............................................................................................................ 14

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5 TROUBLESHOOTING GUIDE.......................................................................................................15

6 SPECIFICATIONS....................................................................................................................................16

7 OBSERVATION MAGNIFICATIONS AND OBSERVATION AREAS................................................18

8 ASSEMBLY....................................................................................................................................................19
8-1 Assembly Diagram..........................................................................................................................................19
8-2 Detailed Assembly Procedure............................................................................................................. 20

9 OPERATION OF OTHER MODULES....................................................................................... 23


9-1 Eyepoint Adjuster SZX-EPA.................................................................................................................... 23
9-2 Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600..........24
9-3 Revolving Nosepiece SZX2-2RE10................................................................................................. 25
9-4 BX Stage Adapter Type 1 SZX-STAD1.......................................................................................... 28
9-5 BX Stage Adapter Type 2 SZX-STAD2.......................................................................................... 29
9-6 Stage Adapter Type 1 SZH-STAD1................................................................................................... 31
9-7 Gliding stage SZH-SG................................................................................................................................. 32
9-8 Cup stage SZH-SC......................................................................................................................................... 33

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SZX10
Important

Safety Precautions

1. After the equipment has been used in an observation of a


specimen that is accompanied with a potential of infection, clean
the parts coming in contact with the specimen to prevent infection.
· Moving this microscope is accompanied with the risk of
dropping the specimen and causing infections. Be sure to
remove the specimen before moving this product.
· In case the specimen is damaged by erroneous operation,

a promptly take the infection prevention measures.


· The microscope may become unstable when certain
intermediate attachments and/or photography unit are
mounted on it. Take the measures so that the microscope does
not turn over.
2. To prevent the microscope body from turning over, its left-right pivot
angle must be limited to 30° as shown in figure. The tilt of the
desktop surface should be no more than 5°.
3. Sufficient care is required during observation with a low-
magnification objective, because the objective’s long WD (Working
Distance) can displace the microscope body to a higher position.
The measures for prevention of turning over are also required when
using an auxiliary pillar (SZH-P400/P600), because this also makes
the microscope body to be displaced to a higher position.
4. To adjust the microscope body height, be sure to hold the
microscope body with one hand while loosening the focusing
assembly clamping knob a .
(Use the drop prevention collar (SZX-R) to prevent a hazard from
occurring.)
Be careful not to pinch your finger during the adjustment.

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1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and
avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight,
high temperature and humidity, or vibration. (For the operating
environment, see “6 Specifications” (P. 16).)
3. When moving the microscope, remove the specimen in advance.
Then, hold the front part of the base with one hand and hold
the rear part of the base with the other hand to avoid tilting the
microscope.
Also remove any module or attachment from the microscope to
minimize the weight before moving.
4. Observe the following cautions when operating the coarse focus
adjustment knob or the zooming knob.

Manipulated
Operation Caution
Controls
Focusing Coarse/fine focus 1. If the knob hits the upper or lower
adjustment knobs limiting mechanism violently or
b a it is rotated after it hits a limiting
c mechanism, the internal mechanism
may be damaged.
2. If the knobs on the left and right
a are rotated in opposite directions,
the internal mechanism will be
damaged. (The rotation tension
of the coarse focus adjustment
knob should be adjusted using the
rotation tension adjustment ring c
on the knob.)
Zooming Zooming knob 1. If the knob hits the upper or lower
b limiting mechanism violently or
it is rotated after it hits a limiting
mechanism, the internal mechanism
may be damaged.
2. If the knobs on the left and right
are rotated in opposite directions,
the internal mechanism will be
damaged.

5. Only one intermediate attachment can basically be used, but two


attachments may also be used though the peripheral part of the
image may be obscured.
However, the coaxial vertical illuminator (SZX2-ILLC10) is not
considered to be an intermediate attachment.
TIP When more than one intermediate attachment is to be
combined, they should be stacked according to the following
order, from the bottom to the top: SZX2-ILLC10, SZX-RFA, SZX-
SDO2, SZX-EPA.

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SZX10

2 Maintenance and Storage


1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
2. If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available
absolute alcohol.
CAUTION Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for
example, electrical equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
3. The equipment uses plastic resins extensively in its external finish. Do not attempt to use organic solvents to clean
them but simply wipe them using a lint-free, soft cloth. To clean an extremely dirty part, use a soft cloth lightly moistened
with a diluted neutral detergent.
4. Never disassemble any part of the microscope as this could result in malfunctions or reduced performance.
5. When not using the microscope, keep it covered with the dust cover provided. If a module containing a source of heat
is mounted on the microscope, wait until it has cooled down before covering.
6. When disposing of the microscope. Check the regulations and rules of your local government and be sure to observe
them.

3 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
microscope may also be damaged. Always use the microscope as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION : Indicates that failure to follow the instructions in the warning could result in
bodily harm to the user and/or damage to equipment (including objects in the
vicinity of the equipment).
NOTE : Indicates that failure to follow the instructions could result in damage to equipment.

TIP : Indicates commentary (for ease of operation and maintenance).

4 Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological
information at hospitals or laboratories.
Typical field of applications are genetics, human blood and tissue examination, neurology, pharmacology and cellular biology.
Further applications of this device include measurement and imaging for materials research, precision manufacturing, electronics
design and medical device fabrication. Additional industrial applications are added by individual companies and researchers.
Do not use this product for any purpose other than its intended use.

This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical
devices. CE marking means the conformity to the directive.
This product is applied with the requirements of EMC standard IEC/EN61326-2-6 and IEC/EN61326-1
concerning electromagnetic compatibility.
This product complies with the emission and immunity requirements described in IEC61326 series. The
electromagnetic environment should be evaluated prior to operation of this product.

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1 Nomenclature
TIP The following illustration shows a typical system composed of modules marked with " " in the list of each
module, and other modules may also be used in place of them. For the modules that are not shown in the
module lists below, please contact Olympus or the latest catalogues.

Eyepieces Observation tube


WHSZ10X-H Binocular observation tube : SZX-BI30/BI45
WHSZ15XH Trinocular observation tube : SZX2-TR30/TR30PT
WHSZ20X-H Tilting trinocular observation tube : SZX2-TTR/TTRPT
WHSZ30X-H Ergonomic long tilting trinocular: SZX2-LTTR*

Focusing assembly
Coarse/fine focusing assembly : SZX2-FOF
Focusing assembly : SZX2-FO
Coarse/fine focusing assembly
for heavy load : SZX2-FOFH

Microscope body
10X zoom microscope body :
Intermediate attachments
SZX2-ZB10
Eyepoint adjuster : SZX-EPA
Extendable eyepoint adjuster:
SZX2-EEPA*
Coaxial vertical illuminator :
SZX2-ILLC10*
Vertical fluorescent light illuminator :
SZX-RFA
Side-by-side viewing attachment:
SZX-SDO2*
Other

Base
Objective Standard base : SZX2-ST**
DFPL0.5X-4 Large base : SZX2-STL**
DFPL0.75X-4 LED transmitted light illumination:
DFPLAPO1X-4 SZX2-ILLTQ*
SZX-ACH1X SZX2-ILLTS*
DFPLAPO1.25X Stage plate
SZX-ACH1.25X Monochrome plate : SZ2-SPBW/SP-BW2
DFPL1.5X-4 Glass plate : SP-C
DFPL2X-4 Fluorescence center plate : SP-FL

** A separate instruction manual is available.


** The standard base (SZX2-ST) requires use of the optional auxiliary pillar (SZH-P400 or SZH-P600) and optional
drop prevention collar (SZX-R). The large base (SZX2-STL) comes with the auxiliary pillar (SZH-P400) mounted as
standard. This enables the large base to be used as is. However, be sure to use the drop prevention collar (SZX-R) in
combination.

Olympus classifies SZX2-ZB10 as an optical microscope and other units as optical microscope accessories.

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SZX10
2 Controls
TIP If the microscope is not yet assembled, see “8 Assembly” (P. 19) before the following.

Light path selector knob (P.13)


Trinocular tube only.

Diopter adjustment ring (P.10) Zooming knob


±5 m-1 (per meter) 0.63X to 6.3X

Coarse focus adjustment knob rotation


tension adjustment ring (P.7)

Eyepiece clamping knob Focusing assembly


clamping knob

Aperture iris diaphragm ring (P.8)

Fine focus adjustment knob


(SZX2-FOF/FOFH)
Stroke : 80 mm
Click stop ON-OFF screw (P.8)
Stroke per turn : 0.77 mm
Engages or disengages the click
stop function for each zoom
magnification.

Coarse focus adjustment knob


Total stroke : 80 mm
Stroke per turn (SZX2-FOF/FOFH) : 36.8 mm
Stroke per turn (SZX2-FO) : 21 mm
Zoom magnification indication (P.9)
0.63 to 6.3 (11 indications)

Specimen holder
Locks the specimen.

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3 Summary Of Observation Procedure
3-1 Preparation

Ref. Page
1. Check and tighten the connection of each component, especially the observation tube............................................................................(Page 21)
2. Check that the angled formed by the microscope body with respect to the base is less
than the turning-over prevention angle............................................................................................................................................................................................................................(Page 20)
3. Adjust the rotation tension of the coarse focus adjustment knob....................................................................................................................................................(Page 7)
4. Confirm the correct settings.

3-2 Observation Procedure

1 Place a specimen on the stage. (Page 7)


2 Adjust the interpupillary distance. (Page 10)

3 Make diopter adjustment of the eyepieces. (Page 10)

( The adjustment procedure is variable depending on whether


the eyepiece micrometer disk is used or not. )

4 Set the zooming knob a to the lowest zoom magnification and bring
a
the microscope into focus by rotating the coarse focus adjustment
knob b .
b
5 Rotate the zooming knob a to the desired magnification and
precisely focus the microscope on the specimen with the coarse
focus adjustment knob b and fine focus adjustment knob c (the
c fine focus adjustment knob is not provided with the SZX2-FO).

TIP The contrast of the observed image and the focal depth of the
specimen can be adjusted with the aperture iris diaphragm
ring.

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SZX10
4 Using The Controls
4-1 Base

1 Using the Stage Plate


In reflected light observation, the stage plate can be placed either the
white or black side facing up.

NOTE Win transmitted light observation, use the transparent


glass stage plate (SP-C).

2 Placing the Specimen


1 Place the specimen on the approximate center of the stage plate. Hold
the specimen with the specimen holder as required.

2 Illuminate the specimen with an illuminator selected according to the


specimen under observation.

4-2 Microscope Body and Focusing Assembly

1 Adjusting the Rotation Tension of the Coarse


1 Focus Adjustment Knob
c

TIP This operation is intended to facilitates the rotation of


the knobs while preventing the spontaneous drop of the
b microscope body. It is recommended to set the knob
rotation tension to a slightly higher level than the point where
spontaneous drop occurs.
If the knobs are hard to move because of the weight of option
a modules and/or camera mounted on the microscope body, it
is recommended to use the focusing assembly for heavy load
(SZX2-FOFH).

NOTE The rotation tension of the coarse focus adjustment knob


can be adjusted with the rotation tension adjustment
ring a . Do not rotate the knobs on the left and right
in opposite directions, for this will damage the internal
mechanism.
1 Rotate the rotation tension adjustment ring a by inserting the
Allen screwdriver c into the hole b on the ring periphery.
Rotating the ring clockwise increases the rotation tension of the coarse
focus adjustment knob, and rotating counterclockwise decreases it.
NOTE · If the microscope body falls down by its own weight or
the focus obtained by fine focusing is lost immediately, the
rotation tension adjustment may be too light. In this case,
rotate the ring clockwise to increase the rotation tension.
· If the rotation tension adjustment is too tight, delicate
focusing will be impossible and the knob may be damaged.
Particularly, to prevent damage, never rotate the fine focus
adjustment knob quickly while its rotation tension is
extremely tight.

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2 Engaging and Disengaging the Zooming Knob
2 Click Stop Function
TIP When the click stop knob is set to ON, the click stop function
is engaged for each magnification indicated with the zooming
b knob. When the knob is set to OFF, the zoom magnification
a can be varied continuously and finely near the click groove.
The click stop knob has been set to OFF before the
microscope is shipped from the factory.

TIP A click stop is provided for each of the 9 intermediate


indication positions between 0.63X and 6.3X of the zoom
microscope body.

1 To engage the click stop function, rotate the click stop ON-OFF screw a
fully clockwise (in the direction of the arrow) using the Allen screwdriver.
The zooming knob then stops at every position corresponding to the
magnification indicated on the zoom magnification indication b .

2 To disengage the click stop function, rotate the click stop ON-OFF
screw a fully by three turns from the ON position, in the direction
opposite to the arrow, using the Allen screwdriver.

NOTE Do not rotate the screw too much, or the cover may be
damaged.

3 Adjusting the Aperture Iris Diaphragm


TIP Adjusting the aperture iris diaphragm increases the contrast of
the observed image as well as the depth of focus.
However, setting the aperture iris diaphragm too narrowly
degrades resolution.

1 Adjust the aperture iris diaphragm ring a to the left or right.


a
Rotating the ring toward the left ( ) opens the aperture; rotating it
toward the right ( ) closes it. Adjust while monitoring the observed
image to confirm the contrast and focal depth improvement effects.

NOTE Do not close the aperture too much, for this may cause
degradation in resolution and/or lack of ambient light.
2 Use the scale gradations as references for memorizing the ring position.
NOTE When the microscope is combined with the coaxial
vertical illuminator (SZX2-ILLC10), minimizing the aperture
may obscure a part of the observed field of view. In this
case, open the aperture to an intermediate position.

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SZX10

4 Zoom Magnification Indication

The body zoom magnification a is indicated on the zooming knob


on the right side.
a The total magnification of observation can be calculated with the
following formula:
Objective Body zoom Eyepiece
x x
magnification magnification magnification

NOTE The magnification indication ring of an objective other


than the 1X objective cannot be used.

Zoom Magnification Indication Sticker (DFPLAPO1.25X Only)

TIP · The DFPLAPO1.25X objective is provided with a sticker that


indicates the magnification*.
a * Indicates the magnification when combined with the body.

· Affix the sticker securely to the knob. Otherwise, the sticker


may peel off during use.
· Affix the sticker while paying attention to the roundness of
the knob.
· The zoom magnification indication sticker can only be
affixed to the right zooming knob.

NOTE Affixing of the sticker blocks the body zoom


magnification indication of a 1X objective.
1 Turn the zooming knob to align the minimum magnification (“0.63”)
with the marking.

2 Peel the back off the sticker a and affix it so that the “0.63"
indication the right zooming knob is overlaid on the “0.8” indication
of the sticker a .

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4-3 Observation Tube

a 1 Adjusting the Interpupillary Distance

NOTE Be sure to hold the binocular assembly a with both


hands to make this adjustment.
To prevent damaging the mechanism, do not apply an
excessive force beyond the stop position.
While looking through the eyepieces, hold the left and right of the
binocular assembly a and adjust the eyepieces by opening or
closing them for binocular vision until the left and right fields of view
coincide completely.

b 2 Adjusting the Diopter (Zoom Parfocal Adjustment)


c
TIP Ensure that the eyepiece clamping knob a is tightened
before starting the adjustment.
Adjusting the diopter specific to the two eyes of each
d
observer allows the parfocality to be ensured across zoom
magnifications.
a
When Not Using the Eyepiece Micrometer Disk

1 Look into the right eyepiece and rotate its diopter adjustment ring b
so that the peripheral part of the visual field looks sharply.

2 Place an easy-to-observe specimen on the stage plate.


3 Rotate the zooming knob c to a low magnification and, looking only
into the right eyepiece, focus the specimen using the coarse and fine
focus adjustment knobs.

4 Rotate the zooming knob c to the highest magnification and, looking


only into the right eyepiece, focus the specimen using the coarse and
fine focus adjustment knobs.

5 Rotate the zooming knob c to the lowest magnification, look only


into the left eyepiece, and focus the specimen by rotating the left
diopter adjustment ring d instead of the coarse and fine focus
adjustment knobs.

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SZX10

When Using the Eyepiece Micrometer Disk

1 Look into the right eyepiece that includes the eyepiece micrometer
disk, and focus the micrometer disk by rotating the diopter adjustment
ring b .

2 Place an easy-to-observe specimen on the stage plate.


3 Rotate the zooming knob c to the highest magnification
and, looking only into the right eyepiece, focus the specimen
using the coarse and fine focus adjustment knobs.
Ensure that both the eyepiece micrometer disk and specimen are
focused accurately.

4 Rotate the zooming knob c to the lowest magnification, look only


into the left eyepiece, and focus the specimen by rotating the left
diopter adjustment ring d instead of the coarse and fine focus
adjustment knobs.

TIP Note (or memorize) the diopter readings of the left and right
eyepiece scales so that they can be duplicated quickly in the
next observation.

3 Using the Eye Shades


When Wearing Eyeglasses

Use with eye shades in their normal folded-down position. (This will
prevent eyeglasses from being scratched by the eyepiece.)

When Not Wearing Eyeglasses

Extend the folded eyeshades in the direction of the arrow. This makes
observation easier by preventing the inverse incidence of light from
between the eyepiece and your eyes.

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d 4 Mounting the Eyepiece Micrometer Disk
a
TIP A variety of eyepiece micrometer disks a can be inserted
b into the WHSZ10X-H, WHSZ15X-H and WHSZ20X-H
eyepieces. Please purchase an eyepiece micrometer disk
with a diameter of 24 mm and thickness of 1.5 mm.

c 1 Unscrew the micrometer disk frame b by turning it counterclockwise


WHSZ10X-H to remove it from the bottom of the eyepiece.

2 Clean the eyepiece micrometer disk a to remove dust and dirt, then
place the disk into the micrometer disk frame b so that the side with
reticule c faces downward.

3 Attach the micrometer disk frame b containing the eyepiece


micrometer disk a by gently screwing it onto the eyepiece d .
TIP · The micrometer disk frame may be tight for certain micrometer
disks. In this case, turn the frame by holding the circumference
with a light, uniform force or by applying the frame against a
rubber sheet. Do not grasp the frame with a strong force, as this
may deform the frame and make it harder to remove it.
· Be careful not to touch the lens surface with your finger.

NOTE The WHSZ20X-H eyepiece is designed to apply a


magnification to the focusing plane of the micrometer
disk.
As the magnification coefficient is 1.35, be sure to perform
magnification compensation when using this eyepiece in
measurement.
When the micrometer disk is engaged in their light path,
the light path length will extend and the diopter scale may
deviate toward the + direction from the normal indication.
However, this does not pose any problem in actual
observation.
TIP When the eyepiece micrometer disk a is not used, wrap it in
a clean sheet of soft paper before storage.

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SZX10

5 Selecting the Light Path of the Trinocular Tube


a

TIP Pull out the light path selector knob a to set the light path
for the right eyepiece to the camera light path. (The following
table shows the intensity ratio of each light path in %.)

Trinocular Tube Pushed-in Pulled-out


SZX2-TR30 Binocular 50%
Right
SZX2-TTR Binocular 100% Camera 50%
( ) Left Binocular 50%
SZX2-TR30PT Right Camera 100%
Binocular 100%
SZX2-TTRPT
Left Camera 100% Binocular 100%
( )

NOTE Always push or pull the light path selector knob a all the
way into a stop position. Do not attempt to force the knob
past the stop position. Applying excessive force could
destroy the mechanism.

6 Adjusting the Tilt


TIP Adjust the height and tilt of the observation tube to the most
comfortable viewing position.
Holding the binocular assembly with both hands, raise or
lower it to the desired position.

NOTE Do not attempt to force the binocular assembly past the


upper or lower stop position. Applying excessive force
could destroy the mechanism.

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4-4 Camera Observation and Photomicrography

TIP The camera observation and photomicrography are available when the SZX2-TR30/TR30PT/TTR/TTRPT
trinocular observation tube is used.

A digital camera unit can be mounted on the straight tube mount of the trinocular tube by means of the camera
adapter and/or camera mount adapter*.
* The camera mount adapter is not necessary if a camera adapter equipped with a camera mount is used.
For details, please also read the instruction manuals for the camera adapter and digital camera.

1 Selecting the Camera Adapter Magnification


Set the magnification of the camera adapter according to the size of the CCD in the digital camera.
(Example) The following figures show the camera observation/photomicrography areas when the WHSZ10X
eyepieces with FN 22 are used.

1 in. CCD

2/3 in. CCD

1/2 in. CCD

WHSZ10X
FN 22
When the 0.5X camera adapter is used When the 1X camera adapter is used

2 Mounting the Camera Adapter


a
1 Using the Allen screwdriver, fully loosen the straight tube clamping
b
c screw a of the straight tube mount on the top of the trinocular tube.

2 Fit the round dovetail b of the camera adapter into the straight tube
mount of the trinocular tube, and tighten the clamping screw a .

3 Mount the camera on the camera adapter. A camera mount adapter


may be required with certain camera adapters.

3 Selecting the Camera Light Path

Pull out the light path selector knob c to select the Binocular 50%/
Camera 50% light path setting for the SZX2-TR30/TTR or the Camera
100% light path setting for the SZX2-TR30PT/TTRPT.

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SZX10
5 Troubleshooting Guide
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If a problem
occurs, please review the following list and take remedial action as needed. If you cannot solve the problem after
checking the entire list, contact Olympus.

Problem Cause Remedy Page

1. The left and right fields of view The interpupillary distance is Adjust it correctly.
10
do not coincide. adjusted improperly.
The diopter is adjusted incorrectly. Adjust it correctly. 10
The left and right eyepieces are Use a pair of matched eyepieces.
22
different.
2. The field of view is obscured The aperture iris diaphragm is Open the aperture iris diaphragm.
8
partially or illuminated unevenly. stopped down excessively.
The trinocular observation tube Mount them properly.
and/or intermediate attachments 21
are installed improperly.
The light path selector knob is in Set it correctly to the desired
13
an intermediate position. position.
3. The monitor image is cut off The light path selector knob is Pull it out all the way.
13
partially. pulled out incompletely.
4. Dirt or dust is visible in the field Dirt/dust on the specimen. Clean thoroughly. 3
of view.
Dirt/dust on eyepiece. Clean thoroughly. 3
5. Details of observed image are The aperture iris diaphragm is Open the aperture to a proper
8
solid. stopped down excessively. diameter.
6. Visibility of observed image is The objective is tilted. Screw it correctly until it stops. 21
poor.
The objective is dirty. Clean thoroughly.
· Image is not sharp.
· Contrast is insufficient. The top and/or bottom lenses of 3
the microscope body are dirty
7. Zooming causes defocusing of The eyepiece diopter is adjusted Adjust it correctly.
10
the observed image. improperly.
The focus adjustment is in Adjust focus at a high
10
accurate. magnification.
8. The coarse focus adjustment The rotation tension of the knob is Decrease the rotation tension to
7
knob does not rotate smoothly set too high. an optimum level.
9. The microscope body drops The rotation tension of the coarse Increase the rotation tension to an
spontaneously, causing the focus adjustment knob is set too optimum level. 7
focusing to be deviated during low.
observation. The microscope body dropped Use a focusing assembly
spontaneously because the weight withstanding heavy loads. (SZX2-
16
exceeded 10 kg. FOF: 2.7 to 15 kg. SZX2-FOFH: 8
to 25 kg.)

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6 Specifications

Item Specifications

1. Zoom microscope body Left/right zoom magnification system.


· SZX2-ZB10 Zoom drive system: Horizontal knob.
Click stop ON-OFF switchable per zoom magnification.
Zoom ratio: 10 (0.63X to 6.3X)
Magnification indications: 0.63, 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3.
Objective mount: Threaded mount
Built-in aperture iris diaphragm.
2. Focusing assembly SZX2-FOFH SZX2-FOF SZX2-FO
· SZX2-FOFH
Focusing system: Rack & pinion roller guide
· SZX2-FOF
(with coarse focus adjustment knob rotation tension adjustment ring).
· SZX2-FO
Built-in gas spring counter Built-in counter balance.
balance.
Coaxial coarse/fine focus Coaxial coarse/fine focus Coarse focus adjustment
adjustment knobs. adjustment knobs. knob only.
Coarse focus adjustment knob stroke: 80 mm
Coarse knob stroke per turn:
Coarse knob stroke per turn: 36.8 mm
21 mm
Fine focus adjustment knob stroke: 80 mm
Fine knob stroke per turn: 0.77 mm
Load: 8 to 25 kg Load: 2.7 to 15 kg Max. load: 10 kg
3. Observation tube SZX-BI30/BI45
· SZX-BI30/BI45
Binocular observation tube
· SZX2-TR30/TR30PT
· SZX2-TTR/TTRPT Tube inclination: 30°/45°
Interpupillary distance adjustment: 50 to 76 mm.
Eyepieces: WHSZ10X-H/15X-H/20X-H/30X-H
SZX2-TR30/TR30PT SZX2-TTR/TTRPT
Trinocular observation tube Tilting trinocular observation tube
Tube inclination: 30° Tube inclination: 5° to 45°
Light path selection: 2 steps.
Light intensity ratios: Binocular 100%, Binocular 50% / Camera 50%
(PT types): Binocular 100%, Camera 100%
Interpupillary distance adjustment: 52 to 76 mm.
Eyepieces: WHSZ10X-H/15X-H/20X-H/30X-H
4. Standard base Pillar support sleeve height: 270 mm.
· SZX2-ST Base dimensions: 284(W) x 335(D) x 31(H) mm.
Specimen holder attachable.
With stage adapter mounting holes.
5. Large base Pillar support sleeve height: 400 mm.
· SZX2-STL Base dimensions: 400(W) x 350(D) x 28(H) mm.
Specimen holder attachable.
With stage adapter mounting holes.
Drop prevention collar: Optional SZX-R.

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Item Specifications

6. Objectives DFPL0.5X-4 WD 171 mm*


DFPL0.75X-4 WD 116 mm
WD : Working distance DFPLAPO1X-4 WD 81 mm
PF : Parfocal lens SZX-ACH1X WD 90 mm
DFPLAPO1.25X WD 60 mm
SZX-ACH1.25X WD 68 mm
DFPL1.5X-4 WD 45.5 mm
DFPL2X-4 WD 33.5 mm
7. Eyepieces WHSZ10X-H** FN 22, diopter adjustment ring provided.
WHSZ15X-H** FN 16, diopter adjustment ring provided.
(Note) The micrometer area
outside the FN is invisible. WHSZ20X-H** FN 12.5, diopter adjustment ring provided.
WHSZ30X-H FN 7, diopter adjustment ring provided.
8. Operating environment ·· Indoor use.
·· Altitude: Max. 2,000 m.
·· Ambient temperature: 5°C to 40°C. (41°F to 104°F)
·· Maximum relative humidity 80% for temperatures up to 31°C (88°F), decreasing
linearly through 70% (at 34°C), 60% (at 37°C) to 50% (at 40°C).

* Auxiliary pillar is required when the SZX2-ST is used.


** Ø24 mm dia., 15 mm thick eyepiece micrometer disk can be inserted.

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7 Observation Magnifications And Observation Areas
·· Observation magnification = Objective magnification x Zoom magnification x Eyepiece magnification
·· Observation area = Eyepiece FN / (Objective magnification x Zoom magnification) (mm)

Eyepieces
WHSZ10X-H WHSZ15X-H WHSZ20X-H WHSZ30X-H
Objective
FN 22 FN 16 FN 12.5 FN 7
Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area
3.15X 69.8 4.725X 50.8 6.3X 39.7 9.45X 22.2
DFPL0.5X-4
31.5X 7.0 47.25X 5.1 63X 4.0 94.5X 2.2
4.73X 46.6 7.09X 33.9 9.45X 26.5 14.18X 14.8
DFPL0.75X-4
47.25X 4.7 70.88X 3.4 94.5X 2.6 141.75X 1.5
DFPLAPO1X-4 6.3X 34.9 9.45X 25.4 12.6X 19.8 18.9X 11.1
SZX-ACH1X 63X 3.5 94.5X 2.5 126X 2.0 189X 1.1
DFPLAPO1.25X 7.88X 27.9 11.81X 20.3 15.75X 15.9 23.63X 8.9
SZX-ACH1.25X 78.75X 2.8 118.13X 2.0 157.5X 1.6 236.25X 0.9
9.45X 23.3 14.8X 16.9 18.9X 13.2 28.35X 7.4
DFPL1.5X-4
94.5X 2.3 141.75X 1.7 189X 1.3 283.5X 0.7
12.6X 17.5 18.9X 12.7 25.2X 9.9 37.8X 5.6
DFPL2X-4
126X 1.7 189X 1.3 252X 1.0 378X 0.6

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SZX10
8 Assembly
8-1 Assembly Diagram

The diagram below shows how to assemble the various modules. The numbers indicate the order of assembly.

NOTE When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid
scratching any part.

Eyepieces Observation tube


WHSZ series SZX-BI30/BI45
SZX2-TR30/TR30PT
SZX2-TTR/TTRPT
6
Intermediate
attachment
SZX-EPA
SZX2-ILLC10
5 etc.

Zoom microscope body


SZX2-ZB10

Required tools 3

Allen screwdriver*
(provided with the zoom Focusing assembly
4 SZX2-FOF
microscope body) SZX2-FO
Allen wrench** SZX2-FOFH
(provided with the standard
base and focusing assembly) Objective
Objective series
for SZX10
* The Allen screwdriver can be stored in the 2
pillar.
** Do not use the Allen wrench for a purpose Allen screwdriver storage hole*
other than installing the pillar and the zoom
Stage plate
microscope body. Pillar
(Provided with the SZX2-ST)
Specimen holder
S-CL
7
8 1

Standard base
SZX2-ST

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8-2 Detailed Assembly Procedure

1 Installing the Pillar


c
b 1 Using the Allen wrench provided with the base, loosen the two
clamping screws a on the pillar support sleeve completely.

2 Hold the pillar b so that the Allen screwdriver storage hole made
of white rubber comes at the top, and insert the pillar into the pillar
a
support sleeve until it reaches the bottom.

3 Tighten the two clamping screws a securely using the Allan wrench.

Reserve Screw Holes

Two screw holes c (6 mm) are provided for use in mounting a


manipulator, etc.

2 Mounting the Focusing Assembly

b
1 First loosen the focusing assembly clamping knob a completely
and, while holding the focusing assembly with both hands, insert the
a
pillar c into the mounting hole b from below.

NOTE Insert vertically and slowly. Do not apply excessive force.


c
2 Lower the focusing assembly until it stops, then tighten the focusing
assembly clamping knob a .

CAUTION To prevent the microscope from turning over, the focusing


assembly must be installed on the same side as the
stage plate in the stand, as shown in the illustration
marked “ ” in figure, and its pivot angle must be limited
to 30°. If the focusing assembly is placed on the wrong
side, the microscope will turn over.
NOTE If the clamping knob a is tightened while the pillar c
30° or less is not completely inserted into the mounting hole b , the
plate spring supporting the pillar will deform and the pillar
will not be able to penetrate into the hole.

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3 Mounting the Microscope Body


a
1 Remove the cap a on the focusing assembly by inserting a thin
object into the notch.

2 Using the Allen wrench, loosen the dovetail mount clamping screw
inside the cap on the focusing assembly by rotating it by 2 or 3 turns
c (counterclockwise).
b
3 Gently insert the dovetail mount c on the rear of the microscope
body into the dovetail mounting port b on the focusing assembly.

NOTE Do not insert the mount at an angle or with excessive


force, for this may cause malfunctions.
4 When the microscope body has been inserted until it stops, tighten
the clamping screw using the Allen wrench.

5 Place the cap a in the original position.

4 Mounting the Objective


a
NOTE Since the objective is heavy, take the following measures
b
to prepare for an accident in case it is dropped.
· Attach the cap to the objective tip.
· Place the cap of the objective mount thread or a
notebook, etc. on the stand to absorb the shock even
when the objective drops.
While holding the tip of the objective b , mount it on the objective
mount thread a by rotating the objective in the direction of the arrow.

5 Mounting the Observation Tube


d
1 Using the Allen screwdriver, loosen the observation tube clamping
c screw a completely.

2 Aligning the positioning pin c of the microscope body to the


b positioning groove b on the observation tube, insert the dovetail
mount d at the bottom of the observation tube into the mounting
a
e port e on the microscope body.

3 Using the Allen screwdriver, tighten the observation tube clamping


screw a .

NOTE The observation tube can be mounted 180° from the


above position, but this positioning makes observation
difficult and is not recommended.
When the auxiliary pillar is used, this positioning is
impossible because the eyepiece gets in the way.

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a 6 Mounting the Eyepiece
TIP When using the micrometer eyepiece disk, insert it in the
right eyepiece. (It may be inserted in the left eyepiece but the
description in this manual assumes that it is inserted in the
right eyepiece.)
1 Remove the eyepiece dust caps a and loosen the eyepiece clamping
c screws b completely.
b
2 Gently insert the eyepieces of the same magnification c into the left
and right eyepiece sleeves all the way until they stop.
3 Tighten both eyepiece clamping screws b .

7 Mounting (Removing) the Stage Plate


Place the stage plate into the mounting hole on the base.
To remove, press the stage plate at the edge nearest to the pillar
with your fingertip. The opposite end will rise from the base so the
stage plate can be picked up easily.

8 Mounting the Specimen Holder


a
TIP Use the specimen holder when you want to hold the
specimen so that it will not move.
Insert the specimen holder a into the two holes b on the
top surface of the base.

9 Positioning the Microscope Body on the Stand


a
Loosen the focusing assembly clamping knob a Slightly pivoting the
microscope body to the left and right, align the center of the objective
with the center of the stage plate b , then clamp the microscope body
b with the focusing assembly clamping knob.

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9 Operation Of Other Modules
9-1 Eyepoint Adjuster SZX-EPA

TIP This module increases the eye point height by 40 mm.


Up to two eyepoint adjusters can be mounted, provided that no other intermediate attachment is used.

1 External View

Observation tube clamping screw

2 Assembly
1 Using the Allen screwdriver provided with the SZX2 microscope body,
a remove the observation tube a .

2 Mount the eyepoint adjuster b to the place where the observation


b tube has been mounted.

3 Mount the observation tube (removed in step 1 above) onto the


eyepoint adjuster.

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9-2 Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600

TIP · The auxiliary pillar is to be used when observing a large specimen or when it is required to move the microscope
body upward for using a low-magnification objective with long WD.
· The drop prevention collar prevents the zoom microscope body from dropping when installed at a high position
using the auxiliary pillar and the clamping knob on the focusing assembly is loosened carelessly. This helps to
avoid accidental damage to the specimen or objective.

NOTE The static load resistance of the SZX-R is 7 kg at maximum.

1 Assembly
1 Mounting the auxiliary pillar
· Remove the pillar from the standard base and replace the pillar with
d the SZH-P400 or SZH-P600 auxiliary pillar (see page 20 for the
mounting procedure).
c 2 Mounting the drop prevention collar
a · Fit the drop prevention collar a onto the auxiliary pillar b .

b TIP Position the oblique illumination unit mounting groove c on


the front side of the drop prevention collar a and tighten the
clamping knob d .
NOTE When the specimen height is low or an objective with
a short working distance is used, the drop prevention
collar a does not have to be mounted if you move the
microscope body downward. However, this reduces safety
that is assured by using the SZX-R drop prevention collar
a , so be always sure to hold the focusing assembly
firmly when loosening the focusing assembly clamping
knob.

2 Operation
CAUTION When moving the microscope body around the pillar, never loosen both the focusing assembly
clamping knob and drop prevention collar simultaneously.

To Move the Microscope Body Upward

1 Loosen the clamping knob of the focusing assembly to be moved upward.


2 At the desired height, tighten the focusing assembly clamping knob securely.
3 Loosen the drop prevention collar clamping knob, press the drop prevention collar tightly against the lower end of the
focusing assembly, and tighten the clamping knob securely.

To Move the Microscope Body Downward

First loosen the drop prevention collar, move the microscope body downward, and then move the focusing assembly.

NOTE For the drop prevention collar to exhibit its full effect, clamp the focusing assembly and drop
prevention collar in close contact between each other, without leaving any space.

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9-3 Revolving Nosepiece SZX2-2RE10

TIP The revolving nosepiece allows two objectives to be mounted. This makes objective switching easy by
simply rotating the revolving nosepiece and expands the range of observation magnifications.

1 External View

Hand guard

Allen wrench
(for M3 screws)

Clamping screws
(M3, 6 mm long, x 3)
Nosepiece mount
Objective mounts

2 Assembly

a 1 Remove the observation tube from the zoom microscope body.


2 Remove the zoom microscope body and focusing assembly from the
b pillar, and place them upside down on a flat desk surface.

NOTE Place a soft sheet made of rubber or similar material on


the desk surface.
3 Remove the objective from the zoom microscope body and then
remove the objective mount b by loosening the three clamping
screws a using the provided Allen wrench (for M3 screws).

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e d 4 Place the revolving nosepiece mount c (with its objective mounts
f
d facing upward) where the objective mount has been by aligning
the screw holes.
Using the Allen wrench (for M3 screws), clamp the revolver mount
with the provided three clamping screws (M3, 6 mm long) e .

NOTE As the screw holes may be hidden behind the objective


mounts d , clamp the nosepiece mount while rotating it.
c 5 Install the focusing assembly on the pillar, and install the observation
tube in the original position.

6 Attach two objectives onto their respective objective mounts d by


screwing.

3 Operation
Hold the objective and gently rotate it until a click position on the front,
where the objective to be used is engaged in the light path.

Operation for Optical Axis Aligned Photomicrography

TIP Align the center of objective and that of the illumination


light path with the photo light path (the right line) to enable
photomicrography with high contrast.
a
1 Rotate the desired objective a clockwise (15°) till the click position
for the photo light path. S

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2 Return the microscope body to the illumination light path by loosening


the focusing module clamping knob b , rotating the microscope body
b gently in the direction of the arrow, aligning visually the objective with
the illumination light path and tightening the clamping knob b again.
Now the setting of the photo light path is complete.

4 Caution
· When transporting the microscope, do not hold it by the revolving nosepiece.

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9-4 BX Stage Adapter Type 1 SZX-STAD1

TIP This adapter is for installation of a U-SRG or U-SRP rotary stage on the SZX2 standard base or a SZX2
series illumination base. When the U-SRP rotary stage is used together with the U-FMP mechanical stage,
X-Y directional movement becomes possible, which is convenient for framing during photomicrography.
To cover the height of the stage adapter (about 44 mm), we recommend combination with the SZH-P400 auxiliary
pillar (together with the SZX-R drop prevention collar).

1 Mountable Bases, Restrictions

Base Applicable Objectives Restrictions


Standard base
0.5X to 2X None
SZX2-ST
· Quad position LED transmitted
light illumination base
SZX2-ILLTQ STAD mount for ILLT SZX2-STADM is necessary for using the stage adapter.
(Refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED transmitted light
· Single position LED transmitted illumination base.)
light illumination base
SZX2-ILLTS

2 Assembly

TIP Use the tools shown in the table below.


b Tool Description
a
Allen wrench (for M4 screw) Provided with SZX-STAD1
Hexagon socket screw (M4) Provided with SZX-STAD1

1 1 Place the notch b of SZX-STAD1 a on the back side of the base,


and secure the screws (2 pcs.) using the Allen wrench to attach SZX-
STAD1 a to a base.

c 2 Attach U-SRP or U-SRG2 to SZX-STAD1 a .


Attaching U-SRP
e Place the positioning pin (cylinder type) c on the back side of the
base, and rotate the centering knob d in clockwise direction to
f secure it.
d
2
Attaching U-SRG2
a
Place the protrusion e on the front side of the base, and rotate the
clamping screw f in clockwise direction to secure U-SRG2 using
the allen screwdriver provided with the microscope frame.

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9-5 BX Stage Adapter Type 2 SZX-STAD2

TIP This adapter is for installing the U-SIC4R2/SIC4L2 large stage* on the SZX2-STL large stand**. When this
stage adapter is used, the SZH-P400 auxiliary pillar should be used to cover the height of the stage adapter
(about 125 mm).
In addition, when a low-magnification objective with a long working distance (DFPL0.5X-4) is used, the
SZH-P600 auxiliary pillar should be used (always together with the drop prevention collar).
* The U-SVL or S-SVR BS stage for the BX series microscopes can also be mounted but the operabilitydeteriorates.
The U-SVLB and U-SVRB cannot be used due to their long stage knobs.
** STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light illumination
base. In addition, the LED transmitted illumination built in SZX2-ILLTQ/ILLTS cannot be used for observation.
For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.

1 Assembly

Large stage
U-SIC4R2/SIC4L2

Frosted filter

3
2

BX stage adapter type 2


SZX-STAD2 Allen wrench
(provided with the base)

1 Clamping screw
Mirror knob

Mounting threaded hole

Large base
SZX2-STL

NOTE For simplified transmitted light observation, place the mirror knob on the front and use a frosted filter.

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2 Simplified Transmitted Light Observation

Microscope 1 Illuminate the specimen with an external light source (LSD illuminator,
body light guide illuminator, etc.).
Objective Light the external light source as shown in the figure on the left and
Frosted filter
irradiate the mirror assembly.
Stage
2 Eliminate irregularities in illumination.

Mirror 1) Align the microscope body center with the center of the SZX-
External STAD2 adapter.
Base light source
2) Set the zooming knob on the microscope body to the minimum
magnification and focus the stage top surface.

3) While observing through the eyepiece, rotate the mirror knob to


adjust the mirror angle so that the entire field of view is illuminated
uniformly.

TIP When observing using an oblique illuminator, remove the


frosted filter and, while observing through the eyepiece, tilt the
mirror gradually until optimum contrast can be obtained.

3 Caution
· Do not project the image of the external light source filament on the frosted surface of the frosted filter.
Otherwise, the frosted filter may deteriorate.
· Use neutral detergent to clean the frosted filter.
· In transmitted light observation at a total magnification of no more than 10X, the field of view may be obscured
in the peripheral sections depending on the stage in use.

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9-6 Stage Adapter Type 1 SZH-STAD1

This adapter has the same function as the SZX-STAD1 BX stage adapter type 1, but the usable stages with this
adapter are the BH2-SH horizontal knob stage.

1 Assembly

TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.

TIP Use the tools shown in the table below.

b Tool Description
Allen wrench (for M4 screw) Provided with SZH-STAD1
a Hexagon socket screw (M4) Provided with SZH-STAD1

1 1 Placing the notch a on the front side of the base, secure the screws
(2 pcs.) using the Allen wrench to attach SZX-STAD1 b to a base.

2 Place the stage clamping knob c of BH2-SH (horizontal knob stage)


on the front of the base, rotate the clamping knob c in clockwise
2 direction to secure it, and attach BH2-SH.

c
b

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9-7 Gliding stage SZH-SG

1 External view and nomenclature


Stage plate
Finger hook

Seat Gliding stage


Illumination field diameter: 40 mm
Moving range diameter: 40 mm

2 Installation
NOTE · Be sure to clean the sliding surface if dirt or metallic power is attached on them.
· Do not place the gliding stage on the friction surface directly on the desktop.
TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.

Stage plate
Use the one provided
with the stand.

Stage plate mount hole 3


Gliding stage

Sliding surface 2 Stage plate mount hole

Seat
1
Applicable stand

TIP Clean the sliding surface periodically.

3 Operation

Hold the gliding stage by the edge and move it horizontally.

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9-8 Cup stage SZH-SC

1 External view and nomenclature

NOTE The SZH-SC can be used only with reflected light illumination. It cannot be used with transmitted light
illumination.
Tube
Finger hook Cup stage
Tilting limit angle: 30°

Stage plate

Specimen holder

Seat
Specimen holder mount holes Same size as the stage plate.

2 Installation

NOTE Before mounting, remove dirt and dust from the mount surfaces and handle carefully so as not to
damage them.
TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.

Specimen holder
Specimen holder mount hole

Stage plate 4
3 Cup stage

Sliding surface Seat


2

Stage plate mount hole


1 Applicable stand

1 Fit the cup stage seat into the stage plate mount hole of an applicable stand.
2 Place the cup stage on the seat. Before placing, wipe the sliding surface on the cup stage and seat with a clean cloth.
3 Mount the stage plate.
4 Mount the specimen holder.
TIP Clean the sliding surface periodically.

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3 Operation

1 Place a specimen on the stage plate, hold the cup stage by the edge,
and tilt the cup stage slowly.

TIP If the specimen slips on the stage plate, hold the specimen
with the provided specimen holder.

To fix a container such as a petri dish, insert the provided tube into
the specimen holder to fix the container by pinching.

NOTE · Do not touch the sliding surface on the cup stage and seat with a hand. If the sliding surface are contaminated
with oil, etc., wash with a neutral detergent before use.
· If an eccentric load of more than 20 grams is applied to the edge of the cup stage, it may move spontaneously.
· When a tall specimen is placed and the cup stage is tilted, the specimen may go out of focus. In this case,
adjust the focus again.

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Manufactured by
Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

Distributed by
EC REP

AX7480 11 Issued in March, 2019


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