SZX 10
SZX 10
SZX 10
This instruction manual is for the Olympus SZX10 Research High-Class Stereo Microscope System. Optical Microscope and Accessory
To ensure the safety, obtain optimum performance and to familiarize yourself full with the use of this
microscope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
For details of products included in the configuration of this system, see “1 Nomenclature” (P. 4)
and “9 Operation Of Other Modules” (P. 23).
IMPORTANT........................................................................................................................................................ 1
1 NOMENCLATURE..................................................................................................................................... 4
2 CONTROLS.................................................................................................................................................... 5
6 SPECIFICATIONS....................................................................................................................................16
8 ASSEMBLY....................................................................................................................................................19
8-1 Assembly Diagram..........................................................................................................................................19
8-2 Detailed Assembly Procedure............................................................................................................. 20
Safety Precautions
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1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and
avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight,
high temperature and humidity, or vibration. (For the operating
environment, see “6 Specifications” (P. 16).)
3. When moving the microscope, remove the specimen in advance.
Then, hold the front part of the base with one hand and hold
the rear part of the base with the other hand to avoid tilting the
microscope.
Also remove any module or attachment from the microscope to
minimize the weight before moving.
4. Observe the following cautions when operating the coarse focus
adjustment knob or the zooming knob.
Manipulated
Operation Caution
Controls
Focusing Coarse/fine focus 1. If the knob hits the upper or lower
adjustment knobs limiting mechanism violently or
b a it is rotated after it hits a limiting
c mechanism, the internal mechanism
may be damaged.
2. If the knobs on the left and right
a are rotated in opposite directions,
the internal mechanism will be
damaged. (The rotation tension
of the coarse focus adjustment
knob should be adjusted using the
rotation tension adjustment ring c
on the knob.)
Zooming Zooming knob 1. If the knob hits the upper or lower
b limiting mechanism violently or
it is rotated after it hits a limiting
mechanism, the internal mechanism
may be damaged.
2. If the knobs on the left and right
are rotated in opposite directions,
the internal mechanism will be
damaged.
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3 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
microscope may also be damaged. Always use the microscope as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
CAUTION : Indicates that failure to follow the instructions in the warning could result in
bodily harm to the user and/or damage to equipment (including objects in the
vicinity of the equipment).
NOTE : Indicates that failure to follow the instructions could result in damage to equipment.
4 Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological
information at hospitals or laboratories.
Typical field of applications are genetics, human blood and tissue examination, neurology, pharmacology and cellular biology.
Further applications of this device include measurement and imaging for materials research, precision manufacturing, electronics
design and medical device fabrication. Additional industrial applications are added by individual companies and researchers.
Do not use this product for any purpose other than its intended use.
This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical
devices. CE marking means the conformity to the directive.
This product is applied with the requirements of EMC standard IEC/EN61326-2-6 and IEC/EN61326-1
concerning electromagnetic compatibility.
This product complies with the emission and immunity requirements described in IEC61326 series. The
electromagnetic environment should be evaluated prior to operation of this product.
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1 Nomenclature
TIP The following illustration shows a typical system composed of modules marked with " " in the list of each
module, and other modules may also be used in place of them. For the modules that are not shown in the
module lists below, please contact Olympus or the latest catalogues.
Focusing assembly
Coarse/fine focusing assembly : SZX2-FOF
Focusing assembly : SZX2-FO
Coarse/fine focusing assembly
for heavy load : SZX2-FOFH
Microscope body
10X zoom microscope body :
Intermediate attachments
SZX2-ZB10
Eyepoint adjuster : SZX-EPA
Extendable eyepoint adjuster:
SZX2-EEPA*
Coaxial vertical illuminator :
SZX2-ILLC10*
Vertical fluorescent light illuminator :
SZX-RFA
Side-by-side viewing attachment:
SZX-SDO2*
Other
Base
Objective Standard base : SZX2-ST**
DFPL0.5X-4 Large base : SZX2-STL**
DFPL0.75X-4 LED transmitted light illumination:
DFPLAPO1X-4 SZX2-ILLTQ*
SZX-ACH1X SZX2-ILLTS*
DFPLAPO1.25X Stage plate
SZX-ACH1.25X Monochrome plate : SZ2-SPBW/SP-BW2
DFPL1.5X-4 Glass plate : SP-C
DFPL2X-4 Fluorescence center plate : SP-FL
Olympus classifies SZX2-ZB10 as an optical microscope and other units as optical microscope accessories.
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2 Controls
TIP If the microscope is not yet assembled, see “8 Assembly” (P. 19) before the following.
Specimen holder
Locks the specimen.
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3 Summary Of Observation Procedure
3-1 Preparation
Ref. Page
1. Check and tighten the connection of each component, especially the observation tube............................................................................(Page 21)
2. Check that the angled formed by the microscope body with respect to the base is less
than the turning-over prevention angle............................................................................................................................................................................................................................(Page 20)
3. Adjust the rotation tension of the coarse focus adjustment knob....................................................................................................................................................(Page 7)
4. Confirm the correct settings.
4 Set the zooming knob a to the lowest zoom magnification and bring
a
the microscope into focus by rotating the coarse focus adjustment
knob b .
b
5 Rotate the zooming knob a to the desired magnification and
precisely focus the microscope on the specimen with the coarse
focus adjustment knob b and fine focus adjustment knob c (the
c fine focus adjustment knob is not provided with the SZX2-FO).
TIP The contrast of the observed image and the focal depth of the
specimen can be adjusted with the aperture iris diaphragm
ring.
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4 Using The Controls
4-1 Base
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2 Engaging and Disengaging the Zooming Knob
2 Click Stop Function
TIP When the click stop knob is set to ON, the click stop function
is engaged for each magnification indicated with the zooming
b knob. When the knob is set to OFF, the zoom magnification
a can be varied continuously and finely near the click groove.
The click stop knob has been set to OFF before the
microscope is shipped from the factory.
1 To engage the click stop function, rotate the click stop ON-OFF screw a
fully clockwise (in the direction of the arrow) using the Allen screwdriver.
The zooming knob then stops at every position corresponding to the
magnification indicated on the zoom magnification indication b .
2 To disengage the click stop function, rotate the click stop ON-OFF
screw a fully by three turns from the ON position, in the direction
opposite to the arrow, using the Allen screwdriver.
NOTE Do not rotate the screw too much, or the cover may be
damaged.
NOTE Do not close the aperture too much, for this may cause
degradation in resolution and/or lack of ambient light.
2 Use the scale gradations as references for memorizing the ring position.
NOTE When the microscope is combined with the coaxial
vertical illuminator (SZX2-ILLC10), minimizing the aperture
may obscure a part of the observed field of view. In this
case, open the aperture to an intermediate position.
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2 Peel the back off the sticker a and affix it so that the “0.63"
indication the right zooming knob is overlaid on the “0.8” indication
of the sticker a .
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4-3 Observation Tube
1 Look into the right eyepiece and rotate its diopter adjustment ring b
so that the peripheral part of the visual field looks sharply.
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1 Look into the right eyepiece that includes the eyepiece micrometer
disk, and focus the micrometer disk by rotating the diopter adjustment
ring b .
TIP Note (or memorize) the diopter readings of the left and right
eyepiece scales so that they can be duplicated quickly in the
next observation.
Use with eye shades in their normal folded-down position. (This will
prevent eyeglasses from being scratched by the eyepiece.)
Extend the folded eyeshades in the direction of the arrow. This makes
observation easier by preventing the inverse incidence of light from
between the eyepiece and your eyes.
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d 4 Mounting the Eyepiece Micrometer Disk
a
TIP A variety of eyepiece micrometer disks a can be inserted
b into the WHSZ10X-H, WHSZ15X-H and WHSZ20X-H
eyepieces. Please purchase an eyepiece micrometer disk
with a diameter of 24 mm and thickness of 1.5 mm.
2 Clean the eyepiece micrometer disk a to remove dust and dirt, then
place the disk into the micrometer disk frame b so that the side with
reticule c faces downward.
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TIP Pull out the light path selector knob a to set the light path
for the right eyepiece to the camera light path. (The following
table shows the intensity ratio of each light path in %.)
NOTE Always push or pull the light path selector knob a all the
way into a stop position. Do not attempt to force the knob
past the stop position. Applying excessive force could
destroy the mechanism.
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4-4 Camera Observation and Photomicrography
TIP The camera observation and photomicrography are available when the SZX2-TR30/TR30PT/TTR/TTRPT
trinocular observation tube is used.
A digital camera unit can be mounted on the straight tube mount of the trinocular tube by means of the camera
adapter and/or camera mount adapter*.
* The camera mount adapter is not necessary if a camera adapter equipped with a camera mount is used.
For details, please also read the instruction manuals for the camera adapter and digital camera.
1 in. CCD
WHSZ10X
FN 22
When the 0.5X camera adapter is used When the 1X camera adapter is used
2 Fit the round dovetail b of the camera adapter into the straight tube
mount of the trinocular tube, and tighten the clamping screw a .
Pull out the light path selector knob c to select the Binocular 50%/
Camera 50% light path setting for the SZX2-TR30/TTR or the Camera
100% light path setting for the SZX2-TR30PT/TTRPT.
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5 Troubleshooting Guide
Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If a problem
occurs, please review the following list and take remedial action as needed. If you cannot solve the problem after
checking the entire list, contact Olympus.
1. The left and right fields of view The interpupillary distance is Adjust it correctly.
10
do not coincide. adjusted improperly.
The diopter is adjusted incorrectly. Adjust it correctly. 10
The left and right eyepieces are Use a pair of matched eyepieces.
22
different.
2. The field of view is obscured The aperture iris diaphragm is Open the aperture iris diaphragm.
8
partially or illuminated unevenly. stopped down excessively.
The trinocular observation tube Mount them properly.
and/or intermediate attachments 21
are installed improperly.
The light path selector knob is in Set it correctly to the desired
13
an intermediate position. position.
3. The monitor image is cut off The light path selector knob is Pull it out all the way.
13
partially. pulled out incompletely.
4. Dirt or dust is visible in the field Dirt/dust on the specimen. Clean thoroughly. 3
of view.
Dirt/dust on eyepiece. Clean thoroughly. 3
5. Details of observed image are The aperture iris diaphragm is Open the aperture to a proper
8
solid. stopped down excessively. diameter.
6. Visibility of observed image is The objective is tilted. Screw it correctly until it stops. 21
poor.
The objective is dirty. Clean thoroughly.
· Image is not sharp.
· Contrast is insufficient. The top and/or bottom lenses of 3
the microscope body are dirty
7. Zooming causes defocusing of The eyepiece diopter is adjusted Adjust it correctly.
10
the observed image. improperly.
The focus adjustment is in Adjust focus at a high
10
accurate. magnification.
8. The coarse focus adjustment The rotation tension of the knob is Decrease the rotation tension to
7
knob does not rotate smoothly set too high. an optimum level.
9. The microscope body drops The rotation tension of the coarse Increase the rotation tension to an
spontaneously, causing the focus adjustment knob is set too optimum level. 7
focusing to be deviated during low.
observation. The microscope body dropped Use a focusing assembly
spontaneously because the weight withstanding heavy loads. (SZX2-
16
exceeded 10 kg. FOF: 2.7 to 15 kg. SZX2-FOFH: 8
to 25 kg.)
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6 Specifications
Item Specifications
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Item Specifications
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7 Observation Magnifications And Observation Areas
·· Observation magnification = Objective magnification x Zoom magnification x Eyepiece magnification
·· Observation area = Eyepiece FN / (Objective magnification x Zoom magnification) (mm)
Eyepieces
WHSZ10X-H WHSZ15X-H WHSZ20X-H WHSZ30X-H
Objective
FN 22 FN 16 FN 12.5 FN 7
Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area
3.15X 69.8 4.725X 50.8 6.3X 39.7 9.45X 22.2
DFPL0.5X-4
31.5X 7.0 47.25X 5.1 63X 4.0 94.5X 2.2
4.73X 46.6 7.09X 33.9 9.45X 26.5 14.18X 14.8
DFPL0.75X-4
47.25X 4.7 70.88X 3.4 94.5X 2.6 141.75X 1.5
DFPLAPO1X-4 6.3X 34.9 9.45X 25.4 12.6X 19.8 18.9X 11.1
SZX-ACH1X 63X 3.5 94.5X 2.5 126X 2.0 189X 1.1
DFPLAPO1.25X 7.88X 27.9 11.81X 20.3 15.75X 15.9 23.63X 8.9
SZX-ACH1.25X 78.75X 2.8 118.13X 2.0 157.5X 1.6 236.25X 0.9
9.45X 23.3 14.8X 16.9 18.9X 13.2 28.35X 7.4
DFPL1.5X-4
94.5X 2.3 141.75X 1.7 189X 1.3 283.5X 0.7
12.6X 17.5 18.9X 12.7 25.2X 9.9 37.8X 5.6
DFPL2X-4
126X 1.7 189X 1.3 252X 1.0 378X 0.6
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8 Assembly
8-1 Assembly Diagram
The diagram below shows how to assemble the various modules. The numbers indicate the order of assembly.
NOTE When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid
scratching any part.
Required tools 3
Allen screwdriver*
(provided with the zoom Focusing assembly
4 SZX2-FOF
microscope body) SZX2-FO
Allen wrench** SZX2-FOFH
(provided with the standard
base and focusing assembly) Objective
Objective series
for SZX10
* The Allen screwdriver can be stored in the 2
pillar.
** Do not use the Allen wrench for a purpose Allen screwdriver storage hole*
other than installing the pillar and the zoom
Stage plate
microscope body. Pillar
(Provided with the SZX2-ST)
Specimen holder
S-CL
7
8 1
Standard base
SZX2-ST
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8-2 Detailed Assembly Procedure
2 Hold the pillar b so that the Allen screwdriver storage hole made
of white rubber comes at the top, and insert the pillar into the pillar
a
support sleeve until it reaches the bottom.
3 Tighten the two clamping screws a securely using the Allan wrench.
b
1 First loosen the focusing assembly clamping knob a completely
and, while holding the focusing assembly with both hands, insert the
a
pillar c into the mounting hole b from below.
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2 Using the Allen wrench, loosen the dovetail mount clamping screw
inside the cap on the focusing assembly by rotating it by 2 or 3 turns
c (counterclockwise).
b
3 Gently insert the dovetail mount c on the rear of the microscope
body into the dovetail mounting port b on the focusing assembly.
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a 6 Mounting the Eyepiece
TIP When using the micrometer eyepiece disk, insert it in the
right eyepiece. (It may be inserted in the left eyepiece but the
description in this manual assumes that it is inserted in the
right eyepiece.)
1 Remove the eyepiece dust caps a and loosen the eyepiece clamping
c screws b completely.
b
2 Gently insert the eyepieces of the same magnification c into the left
and right eyepiece sleeves all the way until they stop.
3 Tighten both eyepiece clamping screws b .
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9 Operation Of Other Modules
9-1 Eyepoint Adjuster SZX-EPA
1 External View
2 Assembly
1 Using the Allen screwdriver provided with the SZX2 microscope body,
a remove the observation tube a .
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9-2 Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600
TIP · The auxiliary pillar is to be used when observing a large specimen or when it is required to move the microscope
body upward for using a low-magnification objective with long WD.
· The drop prevention collar prevents the zoom microscope body from dropping when installed at a high position
using the auxiliary pillar and the clamping knob on the focusing assembly is loosened carelessly. This helps to
avoid accidental damage to the specimen or objective.
1 Assembly
1 Mounting the auxiliary pillar
· Remove the pillar from the standard base and replace the pillar with
d the SZH-P400 or SZH-P600 auxiliary pillar (see page 20 for the
mounting procedure).
c 2 Mounting the drop prevention collar
a · Fit the drop prevention collar a onto the auxiliary pillar b .
2 Operation
CAUTION When moving the microscope body around the pillar, never loosen both the focusing assembly
clamping knob and drop prevention collar simultaneously.
First loosen the drop prevention collar, move the microscope body downward, and then move the focusing assembly.
NOTE For the drop prevention collar to exhibit its full effect, clamp the focusing assembly and drop
prevention collar in close contact between each other, without leaving any space.
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TIP The revolving nosepiece allows two objectives to be mounted. This makes objective switching easy by
simply rotating the revolving nosepiece and expands the range of observation magnifications.
1 External View
Hand guard
Allen wrench
(for M3 screws)
Clamping screws
(M3, 6 mm long, x 3)
Nosepiece mount
Objective mounts
2 Assembly
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e d 4 Place the revolving nosepiece mount c (with its objective mounts
f
d facing upward) where the objective mount has been by aligning
the screw holes.
Using the Allen wrench (for M3 screws), clamp the revolver mount
with the provided three clamping screws (M3, 6 mm long) e .
3 Operation
Hold the objective and gently rotate it until a click position on the front,
where the objective to be used is engaged in the light path.
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4 Caution
· When transporting the microscope, do not hold it by the revolving nosepiece.
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9-4 BX Stage Adapter Type 1 SZX-STAD1
TIP This adapter is for installation of a U-SRG or U-SRP rotary stage on the SZX2 standard base or a SZX2
series illumination base. When the U-SRP rotary stage is used together with the U-FMP mechanical stage,
X-Y directional movement becomes possible, which is convenient for framing during photomicrography.
To cover the height of the stage adapter (about 44 mm), we recommend combination with the SZH-P400 auxiliary
pillar (together with the SZX-R drop prevention collar).
2 Assembly
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TIP This adapter is for installing the U-SIC4R2/SIC4L2 large stage* on the SZX2-STL large stand**. When this
stage adapter is used, the SZH-P400 auxiliary pillar should be used to cover the height of the stage adapter
(about 125 mm).
In addition, when a low-magnification objective with a long working distance (DFPL0.5X-4) is used, the
SZH-P600 auxiliary pillar should be used (always together with the drop prevention collar).
* The U-SVL or S-SVR BS stage for the BX series microscopes can also be mounted but the operabilitydeteriorates.
The U-SVLB and U-SVRB cannot be used due to their long stage knobs.
** STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light illumination
base. In addition, the LED transmitted illumination built in SZX2-ILLTQ/ILLTS cannot be used for observation.
For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.
1 Assembly
Large stage
U-SIC4R2/SIC4L2
Frosted filter
3
2
1 Clamping screw
Mirror knob
Large base
SZX2-STL
NOTE For simplified transmitted light observation, place the mirror knob on the front and use a frosted filter.
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2 Simplified Transmitted Light Observation
Microscope 1 Illuminate the specimen with an external light source (LSD illuminator,
body light guide illuminator, etc.).
Objective Light the external light source as shown in the figure on the left and
Frosted filter
irradiate the mirror assembly.
Stage
2 Eliminate irregularities in illumination.
Mirror 1) Align the microscope body center with the center of the SZX-
External STAD2 adapter.
Base light source
2) Set the zooming knob on the microscope body to the minimum
magnification and focus the stage top surface.
3 Caution
· Do not project the image of the external light source filament on the frosted surface of the frosted filter.
Otherwise, the frosted filter may deteriorate.
· Use neutral detergent to clean the frosted filter.
· In transmitted light observation at a total magnification of no more than 10X, the field of view may be obscured
in the peripheral sections depending on the stage in use.
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This adapter has the same function as the SZX-STAD1 BX stage adapter type 1, but the usable stages with this
adapter are the BH2-SH horizontal knob stage.
1 Assembly
TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.
b Tool Description
Allen wrench (for M4 screw) Provided with SZH-STAD1
a Hexagon socket screw (M4) Provided with SZH-STAD1
1 1 Placing the notch a on the front side of the base, secure the screws
(2 pcs.) using the Allen wrench to attach SZX-STAD1 b to a base.
c
b
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9-7 Gliding stage SZH-SG
2 Installation
NOTE · Be sure to clean the sliding surface if dirt or metallic power is attached on them.
· Do not place the gliding stage on the friction surface directly on the desktop.
TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.
Stage plate
Use the one provided
with the stand.
Seat
1
Applicable stand
3 Operation
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NOTE The SZH-SC can be used only with reflected light illumination. It cannot be used with transmitted light
illumination.
Tube
Finger hook Cup stage
Tilting limit angle: 30°
Stage plate
Specimen holder
Seat
Specimen holder mount holes Same size as the stage plate.
2 Installation
NOTE Before mounting, remove dirt and dust from the mount surfaces and handle carefully so as not to
damage them.
TIP STAD mount for ILLT SZX2-STADM is necessary for using the SZX2-ILLTQ/ILLTS LED transmitted light
illumination base. For details, refer to the instruction manual for the SZX2-ILLTQ/ILLTS LED.
Specimen holder
Specimen holder mount hole
Stage plate 4
3 Cup stage
1 Fit the cup stage seat into the stage plate mount hole of an applicable stand.
2 Place the cup stage on the seat. Before placing, wipe the sliding surface on the cup stage and seat with a clean cloth.
3 Mount the stage plate.
4 Mount the specimen holder.
TIP Clean the sliding surface periodically.
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3 Operation
1 Place a specimen on the stage plate, hold the cup stage by the edge,
and tilt the cup stage slowly.
TIP If the specimen slips on the stage plate, hold the specimen
with the provided specimen holder.
To fix a container such as a petri dish, insert the provided tube into
the specimen holder to fix the container by pinching.
NOTE · Do not touch the sliding surface on the cup stage and seat with a hand. If the sliding surface are contaminated
with oil, etc., wash with a neutral detergent before use.
· If an eccentric load of more than 20 grams is applied to the edge of the cup stage, it may move spontaneously.
· When a tall specimen is placed and the cup stage is tilted, the specimen may go out of focus. In this case,
adjust the focus again.
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Manufactured by
Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan
Distributed by
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