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    PTH 403 Report Updated

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    anand reddy
    This monthly progress report summarizes experiments conducted as part of a Bachelor of Science program in agriculture. The report details procedures for preparing yeast extract media, using the leaf disc method to extract yeast from leaves, visualizing the yeast under a microscope, preparing yeast slants, and growing yeast in molasses media. The goal of the experiments was to learn techniques for producing and observing yeast.

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    PTH 403 Report Updated

    Uploaded by

    anand reddy
    43 views9 pages
    This monthly progress report summarizes experiments conducted as part of a Bachelor of Science program in agriculture. The report details procedures for preparing yeast extract media, using the leaf disc method to extract yeast from leaves, visualizing the yeast under a microscope, preparing yeast slants, and growing yeast in molasses media. The goal of the experiments was to learn techniques for producing and observing yeast.

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    This monthly progress report summarizes experiments conducted as part of a Bachelor of Science program in agriculture. The report details procedures for preparing yeast extract media, using the leaf disc method to extract yeast from leaves, visualizing the yeast under a microscope, preparing yeast slants, and growing yeast in molasses media. The goal of the experiments was to learn techniques for producing and observing yeast.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    43 views9 pages

    PTH 403 Report Updated

    Uploaded by

    anand reddy
    This monthly progress report summarizes experiments conducted as part of a Bachelor of Science program in agriculture. The report details procedures for preparing yeast extract media, using the leaf disc method to extract yeast from leaves, visualizing the yeast under a microscope, preparing yeast slants, and growing yeast in molasses media. The goal of the experiments was to learn techniques for producing and observing yeast.

    Copyright:

    © All Rights Reserved
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    MONTHLY PROGRESS REPORT

    EXPERIMENTAL LEARNING PROGRAMME (ELP)


    PTH 403
    PRODUCTION TECHNOLOGY FOR YEAST

    SUBMITTED IN PARTIAL FULFILMENT OFN THE REQUIREMENT OF


    THE DEGREE
    BACHELOR OF SCIENCE (HONS.) AGRICULTURE

    SUBMITTED BY:
    NAME: B. Mamata
    REG.NO: 11914221
    SECTION: HC252

    SUPERVISOR:
    Dr.DEEWAKAR BARAL
    DEPT. OF PATHOLOGY
    SCHOOL OF AGRICULTURE
    LOVELY PROFESSIONAL UNIVERSITY, PHAGWARA-144411, PUNJAB,
    INDIA
    YEAST EXTRACT MEDIA
    ➢ PREPARATION OF MEDIA

    REQUIREMENTS
    • Conical flasks
    • Yeast extract
    • Agar
    • Glass rod
    • Distilled water
    • Hot plate
    • Cotton

    PROCEDURE
    1. First take 1000 ml of distilled water in the conical flask
    2. Add 23 gm of yeast extract in the water.
    3. Add 7 gm of agar agar into the flask
    4. Now heat the flask on the hot plate until yeast extract and agar agar gets
    dissolved in the water
    5. After complete boiling ,sterilize media by autoclaving at 15lbs (121℃) for
    15 min
    LEAF DISC METHOD

    REQUIREMENTS:
    • YEAST EXTRACT MEDIA
    • LAMIAN AIR FLOW
    • YOUNG LEAVES
    • ANTIBIOTICS
    • SPIRIT LAMP
    • ETHANOL
    • PETRI PLATES

    PROCEDURE:

    1. First on the laminar air flow before starting the experiment and on
    the uv light
    2. Take the young leaves and wash them with the distilled water and
    dry
    3. Now add the autoclaved media in the petri dishes and leave it to dry
    for one minute.
    4. Now cut the leaves in the smaller pieces so that the pieces can be
    placed in triangular format.
    5. After placing all the leaves pack the petri dishes in such a way that
    there will no way to contaminate the media.
    6. Store this in the incubator for the 6 hours.
    7. Now remove all the leaves and pack it and keep in the incubator

    Result
    The next morning you can see the yeast grown in the petri dishes
    By this we can understand that yeast is present in the leaves
    Visualizing the yeast under microscope

    REQUIREMENTS:
    • MICROSCOPE
    • YESAT
    • SLIDES
    • COVERSLIP

    PROCEDURE
    1. Take the slide and wash it properly
    2. Take the yeast from which the yeast was extracted from the leaf disc
    method
    3. Now place the coverslip in which there should be no air bubbles on
    the slide
    4. Now do observe on the microscope

    OBSERVATION:
    PREPARATION OF SLANTS

    REQUIREMENTS:
    • Yeast extract media
    • Test tubed
    • Laminar air flow
    • Inoculation tubes
    • Lamp

    PROCEDURE

    1. Take the sterile test tubes from the autoclave to avoid the contamination
    from bacteria
    2. Pour the yeast extract media in the test tube in such a way that through the
    walls of the test tube
    3. Now leave 2-3 min to dry
    4. Now make the lines in each of the test tubes
    5. Now pack the all the test tubes in the polythene bags cover it properly.
    6. Now keep it in incubator for 6 hrs. and then observe

    Result
    Now you the see the yeast grown on the media from which we have inoculated.
    MOLASSES
    REQUIREMENTS:
    • Molasses
    • Urea
    • Conical flask
    • Water

    PROCEDURE:
    1. Take the 3 conical flasks
    2. Now add 500 ml of water in each of the flask
    3. Now add 2gm ,3gm and 4gm molasses in conical flask 1,2and 3
    respectively
    4. Then start heating on the hot plate so that the molasses get mixed with the
    water completely
    5. Again now place in the autoclave for the 15 min at 15 lbs.
    6. Now place the stants in the incubator for 4-6 hrs.
    Result
    After incubation now you can see the yeast growth on the bottom of the flask

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