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    DNA Extraction Lab Sheet

    Uploaded by

    Strawberry Ro
    Scientists isolate DNA for genetic testing and body identification. This virtual lab simulates DNA extraction from cheek cells. First, cells are collected and lysed with detergent and proteinase K to disrupt membranes and release DNA. Then salt separates DNA from proteins and debris while centrifugation causes heavier components to sink. Isopropyl alcohol precipitates DNA from solution. Centrifugation isolates DNA at the bottom of a new tube. A balanced centrifuge is important for proper function and to prevent damage during high speeds.

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    DNA Extraction Lab Sheet

    Uploaded by

    Strawberry Ro
    96 views1 page
    Scientists isolate DNA for genetic testing and body identification. This virtual lab simulates DNA extraction from cheek cells. First, cells are collected and lysed with detergent and proteinase K to disrupt membranes and release DNA. Then salt separates DNA from proteins and debris while centrifugation causes heavier components to sink. Isopropyl alcohol precipitates DNA from solution. Centrifugation isolates DNA at the bottom of a new tube. A balanced centrifuge is important for proper function and to prevent damage during high speeds.

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    DNA extraction Lab sheet

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    Scientists isolate DNA for genetic testing and body identification. This virtual lab simulates DNA extraction from cheek cells. First, cells are collected and lysed with detergent and proteinase K to disrupt membranes and release DNA. Then salt separates DNA from proteins and debris while centrifugation causes heavier components to sink. Isopropyl alcohol precipitates DNA from solution. Centrifugation isolates DNA at the bottom of a new tube. A balanced centrifuge is important for proper function and to prevent damage during high speeds.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    96 views1 page

    DNA Extraction Lab Sheet

    Uploaded by

    Strawberry Ro
    Scientists isolate DNA for genetic testing and body identification. This virtual lab simulates DNA extraction from cheek cells. First, cells are collected and lysed with detergent and proteinase K to disrupt membranes and release DNA. Then salt separates DNA from proteins and debris while centrifugation causes heavier components to sink. Isopropyl alcohol precipitates DNA from solution. Centrifugation isolates DNA at the bottom of a new tube. A balanced centrifuge is important for proper function and to prevent damage during high speeds.

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    © All Rights Reserved
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    Student Web quest: DNA EXTRACTION Source: Genetic Science Learning Center. "DNA Extraction Virtual Lab.

    "
    Learn.Genetics 14 July 2009
    http://learn.genetics.utah.edu/content/labs/extraction/

    Objective: In this virtual lab, you will identify the resources and process of DNA Extraction.

    Introduction:

    1. Name two (2) reasons scientists isolate DNA.


    Genetic testing
    Body identification
    Step One: Collect Cheek Cells

    2. What is contained inside the nucleus of every cheek cell?

    Two meters of DNA

    Step Two: Burst Cells Open to Release DNA


    To separate
    3. “Lysis” is a Greek word that means what? _____________________________________________________

    4. The Lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent and
    enzymes. What was the purpose of the detergent? What was the purpose of the proteinase K?
    The purpose of the detergent is to disrupt the cell The purpose of proteinase
    membrane and nuclear envelope so cells can burst open K is to cut apart histones to
    and release DNA.
    free DNA.
    Step Three: Separate DNA from Proteins and Debris

    5. What is the function of the salt solution?

    It causes protiens and other cellular debris to clump together.

    6. What is the function of the centrifuge step?


    It spins around really fast so the heavy clumps of protien
    and cellular debris can sink to the bottom of the tube
    and the strand of DNA stays in the liquid.
    Step Four: Isolate Concentrated DNA

    7. Isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean?
    DNA does not dissolve in Isoprophyl alcohol.

    8. Why must the new tube be centrifuged?

    So the DNA can sink to the bottom of the tube.


    Analysis and Conclusions

    9. Why do you think it is important to have a “balanced” centrifuge?


    It might not work how it’s supposed to and since it’s moving really fast something might also break
    because it isn’t balanced.
    10. Relate how shampoo performs the same function on your hair as it does on cell membranes in this lab.

    Shampoo separates the dirt from your hair like how the warm water separates the DNA from
    the cell

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