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Assignment 1 BRD BT5071-2022

The document contains 10 questions regarding bioreactor design and analysis. Question 1 asks about modeling microbial growth kinetics using Monod kinetics. Question 2 involves estimating time to stationary phase and final cell density for a batch culture. Question 3 requires calculating kinetic parameters from experimental batch culture data. The remaining questions involve batch, fed-batch, continuous bioreactor operation and calculations related to substrate conversion, biomass productivity, residence time and annual biomass production.

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Subhrojyoti Ghosh
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146 views3 pages

Assignment 1 BRD BT5071-2022

The document contains 10 questions regarding bioreactor design and analysis. Question 1 asks about modeling microbial growth kinetics using Monod kinetics. Question 2 involves estimating time to stationary phase and final cell density for a batch culture. Question 3 requires calculating kinetic parameters from experimental batch culture data. The remaining questions involve batch, fed-batch, continuous bioreactor operation and calculations related to substrate conversion, biomass productivity, residence time and annual biomass production.

Uploaded by

Subhrojyoti Ghosh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BT5071

BIOREACTOR DESIGN AND ANALYSIS


ASSIGNMENT 1

1. The growth rate of E. coli be expressed by Monod kinetics with the parameters of µ max = 0.935
hr -1 and KS = 0.71 gL-1. Assume that the cell yield YX/S is 0.6 g dry cells per g substrate. If CX0
is 1 gL-1 and CS0 = 10 gL-1. when the cells start to grow exponentially, at t 0 = 0, show how ln
CX, CS, d ln CX /dt, and dCX /dt change with respect to time.

2. A strain of Escherichia coli has been genetically engineered to produce human protein. A batch
culture is started in a 150 L fermenter by inoculating 30 g cells into it. 12 gL-1glucose is added
into the fermenter initially. The maximum specific growth rate of the culture is 0.75 h -1.
Assume that the culture is growing at its maximum specific growth rate and the biomass yield
from glucose is 0.9 gg-1.

a. Estimate the time required to reach the stationary phase.


b. What will be the final cell density if the fermentation is stopped after only 90% of the
substrate is consumed?

3. A batch fermentation of a fungal culture in glucose medium gave the following results

Time (h) Concentration Glucose


(gL-1) Concentration
(gL-1)
0 1.25 100
9 2.45 97
16 5.1 90.4
23 10.5 76.9
30 22 48.1
34 33 20.6
36 37.5 9.38
40 41 0.63

Calculate:
a. Maximum growth rate (μmax)
b. Yield on substrate (YX/S)
c. Doubling time (td)
d. Specific growth rate (μnet) at t = 30 h
4. Daucas carota cells are cultured to high density for product formation. The reactor used
is a stirred tank that initially contains 175 L of medium. The maximum specific
growth rate of the culture is 0.12 day-1 and the yield of biomass from substrate is
0.75 gg-1. The concentration of growth-limiting substrate in the medium is 3%
(w/v). The reactor is inoculated with 2.5 gL-1of cells and operated in batch until the
substrate is virtually exhausted; medium flow is then started at a rate of 4 Lday-1.
Fed-batch operation is carried out for 25 days under quasi-steady-state conditions.

a. Estimate the batch culture time and the final biomass concentration achieved after the
batch culture period.
b. What is the final mass of cells in the reactor?
c. The fermenter is available 275 days per year with a downtime between runs of 20 h.
How much cell biomass is produced annually?

5. A 5 m-3 fermenter is operated continuously with feed substrate concentration 20 kg m-3. The
microorganism cultivated in the reactor has the following characteristics; µ max = 0.45 h-1, KS =
0.8 kg m-3, YX/S =0.55 kg kg-1.

a. What feed flow rate is required to achieve 90% substrate conversion?


b. How does the biomass productivity at 90% substrate conversion compare with the
maximum possible?

6. A two-stage chemostat system is used for production of secondary metabolite. The volume of
each reactor is 0.5 m-3; the flow rate of feed is 50 Lh-1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of substrate in the
feed is 10 g L-1. Kinetic and yield parameters for the organism are: YX/S = 0.5 kg kg-1; KS = 1
kg m-3 ; µmax = 0.12 h-1 ; ms = 0.025 kg kg-1 h -1; qp = 0.16 kg kg-1 h -1, YPS = 0.85 kg kg-1.
Assume that product synthesis is negligible in the first reactor and growth is negligible in the
second reactor.

a. Determine the cell and substrate concentrations entering the second reactor.
b. The overall substrate conversion?
c. What is the final concentration of the product?

7. The volume of a fermenter is increased to 4 times while keeping the cell concentration and
other fermenter conditions the same.

a. How will the volumetric productivity get affected?


b. How will the specific productivity get affected?
c. How will the total productivity get affected?
8. In a fed-batch culture operating with intermittent addition of glucose, the volume is given at
time t = 2 hr, when the system is at quasi-steady state.
Flow rate = 200 ml h-1, V = 1000 ml, S0 = 100 gL1, YX/S = 0.5 g dw cells/ g glucose:
μmax = 0.3 h-1, KS = 0.1 gL-1, Xt0 = 30 g cells

Using above given values of other parameters, determine


a. Initial Reactor volume.
b. Substrate concentration, Biomass concentration at quasi steady state
c. Product concentration at t = 2hr assuming quasi-steady state if q p= 0.2 g product/g cells-
h, P0=0

9. You need to cultivate hypothetical microbial cells with the Monod kinetic parameter values of
μmax = 5.0 hr-1 and KS = 20 g/L. The cell yield (YX/S) is 0.4 and the substrate concentration is
30 g L-1. The required substrate conversion is 97%. Estimate the residence time required for
the following fermenter configurations:
a. Plug-flow fermenter with the inlet cell concentration of 0.5 g L-1.
b. One steady-state CSTR with a recycle stream (no cell separator) with the flow rate of
0.4F. The inlet stream is sterile.

10. Methylomonas methanica is used to produce single-cell protein from methanol in a 1000 m3
fermenter. The biomass yield from substrate is 0.5 g g -1 , KS is 0.7 mg L-1 , and the maximum
specific growth rate is 0.44 h-1 . The medium contains 2% (w/v) methanol. A substrate
conversion of 98% is desirable. The reactor may be operated either in batch or continuous
mode. If operated in batch, an inoculum of 0.1% (w/v) is used and the downtime between
batches is 22 h. If operated continuously, a downtime of 20 days is expected per year. Compare
the annual biomass production from batch and continuous reactors (Neglect maintenance
requirements)

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