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    Bacteriology Mtap (Part 2)

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    Joshua Cayago
    This document provides information on various bacteriology media and their uses, as well as tests for identifying Staphylococcus and Streptococcus species. It lists media such as blood agar, mannitol salt agar, and Baird-Parker agar and what each is selective for. Key identification tests for Staphylococcus include coagulase, heat stable nuclease, and pyrrolidonlyl arylamidase. Streptococcus species are grouped by Lancefield antigens and identified using tests like CAMP and pyrrolidonlyl arylamidase. Characteristics of Neisseria include being diplococci typically found intracellularly in humans.

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    Bacteriology Mtap (Part 2)

    Uploaded by

    Joshua Cayago
    143 views10 pages
    This document provides information on various bacteriology media and their uses, as well as tests for identifying Staphylococcus and Streptococcus species. It lists media such as blood agar, mannitol salt agar, and Baird-Parker agar and what each is selective for. Key identification tests for Staphylococcus include coagulase, heat stable nuclease, and pyrrolidonlyl arylamidase. Streptococcus species are grouped by Lancefield antigens and identified using tests like CAMP and pyrrolidonlyl arylamidase. Characteristics of Neisseria include being diplococci typically found intracellularly in humans.

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    This document provides information on various bacteriology media and their uses, as well as tests for identifying Staphylococcus and Streptococcus species. It lists media such as blood agar, mannitol salt agar, and Baird-Parker agar and what each is selective for. Key identification tests for Staphylococcus include coagulase, heat stable nuclease, and pyrrolidonlyl arylamidase. Streptococcus species are grouped by Lancefield antigens and identified using tests like CAMP and pyrrolidonlyl arylamidase. Characteristics of Neisseria include being diplococci typically found intracellularly in humans.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    143 views10 pages

    Bacteriology Mtap (Part 2)

    Uploaded by

    Joshua Cayago
    This document provides information on various bacteriology media and their uses, as well as tests for identifying Staphylococcus and Streptococcus species. It lists media such as blood agar, mannitol salt agar, and Baird-Parker agar and what each is selective for. Key identification tests for Staphylococcus include coagulase, heat stable nuclease, and pyrrolidonlyl arylamidase. Streptococcus species are grouped by Lancefield antigens and identified using tests like CAMP and pyrrolidonlyl arylamidase. Characteristics of Neisseria include being diplococci typically found intracellularly in humans.

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    BACTERIOLOGY MTAP (PART 2)

    MEDIA USE
    Blood agar medium  Enrichment/Differential
    Mannitol Salt Agar  Selective (7.5% NaCl)
     Differential (Mannitol)
    Columbia CAN agar  Colistin and Nalidixic Acid
     Selective for G+
    Phenylethyl Alcohol (PEA) Agar  Used for determination of hemolytic reactions since
    atypical reactions
    Vogel-Johnson (VJ)  Selectivity (potassium tellurite, lithium chloride and
    glycine)
     Inhibit nonstaphylococcal organisms
    Chapman Stone Agar  Selective (5.5% NaCl)
     Differential (Mannitol and Gelatin)
    Baird-Parker Agar  Selective (tellurite, lithium chloride)
     Demonstrate proteolytic activity

    STAPHYLOCOCCUS

    STAPHYLOCOCCUS
     Lactose Fermenter
     Halotolerant
    BACTERIOLOGY MTAP (PART 2)

    TAXO-A
    BACTERIOLOGY MTAP (PART 2)

    KEY TESTS FOR IDENTIFICATION OF


    STAPHYLOCOCCUS SPECIES

    1. COLONY PIGMENT
     S. AUREUS (+) YELLOW
    6. PYRROLIDONYL ARYLAMIDASE/ PYRase
    2. STAPHYLOCOAGULASE TEST
     S. AUREUS (+)  S. AUREUS (-)
     S. SCHLEIFERI (+)
    3. CLUMPIG FACTOR 7. OTHERS IN THE TABLE ABOVE
     S. AUREUS (+)
     S. LUGDUNENSIS (+)
     S. SCHLEIFERI (+) mimics tests  If the sample is from sterile site,
    for staphylococcus aureus perform SUGAR TEST (the ability of the
    organism to ferment sugar)
    4. HEAT STABLE NUCLEASE
    COAGULASE (+) STAPH DIFFERENTIATION
     S. AUREUS (+) Staph spp. VP PYR
     S. SCHLEIFERI (+) S. aureus + -
    S. schleiferi + +
    5. ALKALINE PHOSPHATASE S. hyicus - -
     S. AUREUS (+) S. intermedius - +
     S. SCHLEIFERI (+) S. delphini - +
    BACTERIOLOGY MTAP (PART 2)
    Cannot be differentiated in VP and
    PYRase test

    STREPTOCOCCUS

    LANCEFIELD GROUP: Grouped according to the presence of sugar in the cell wall which serves as the antigen

    COCOBACILLUS: Streptococcus pneumonia

    Cultural characteristics:

    1. Stab and Streak Method


     performed to assess the hemolytic reaction of the microorganisms when streptococcus is suspected
     Hemolysis is enhanced in anaerobic condition
    2. Colonies with Depressed center
     Characteristic of S. PNEUMONIAE
     Because the organism produces autolytic enzyme
    3. Mucoid colonies
     S. PNEUMONIA can produce capsul

    OPTOCHIN : Enhances the organisms ability to produce AUTOLYSIS AMYDASE that lyses itself
    BACTERIOLOGY MTAP (PART 2)

    CAMP (+): Grp. B


    PYRase (+): Grp. A
    CAMP (-): Grp. D
    BACTERIOLOGY MTAP (PART 2)

    MOT – Motility
    MAN – Manitol
    SOR – Sorbose
    ARA – Arabinose
    RAF – Raffinose
    TEL – Tellurite
    ARG – Arginine
    PYU – Pyruvate
    MGP – Methyl a-D-glucopyranoside
    BACTERIOLOGY MTAP (PART 2)

    NEISSERIACEAE

     NEISSERIACEAE
    BACTERIOLOGY MTAP (PART 2)
    o Only cocci among other Neisseriacae isolated in human
    o Diplococci observed intracellularly located
    BACTERIOLOGY MTAP (PART 2)
    BACTERIOLOGY MTAP (PART 2)

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