Industrial Microbiology & Food Processing
Industrial Microbiology & Food Processing
Industrial Microbiology & Food Processing
Food Processing
Shashank Joshi
Food Microbiology: Basic aspects and scope of food microbiology.
Microorganisms play a big role in the industry, with multiple ways to be used. Microorganisms can be
used for medicine, food, industrial ( non food) and agriculture, many more.
Food Microbiology: Basic aspects and scope of food microbiology.
Food microbiology focuses on the study of microbial ecology related to foods, the
effect of environment on food safety, food spoilage and food manufacture, the
physical, chemical and biological destruction of microorganisms in foods,
control of safety and quality of food stuff, exploration of antimicrobial substances
Microbiology deals with the study of microorganisms and their interactions with
biotic and abiotic components of the environment. Microbes are so small that
they are not seen by the naked eye, and include bacteria and archaea, fungi, algae,
protozoa, and viruses.
Intrinsic and extrinsic factors that affect microbial growth in
foods.
(i) Intrinsic Factors : pH, moisture etc. (ii) Extrinsic Factors : Temperature, Oxygen etc.
Microbial cells require water in an available form to grow in food products. In ancient
time foods are preserved by controlling their moisture content.
Most fresh foods i.e. fresh meat, vegetables, and fruits, have aw values that are close
to the optimum growth level of most microorganisms (0.97 - 0.99) Some foods
intrinsically contain inherent naturally-occurring antimicrobial compounds that can
provide microbiological stability to them.
Intrinsic and extrinsic factors that affect microbial growth in
foods.
1. Microorganisms have need of certain basic nutrients for their growth and maintenance of physiological functions.
These nutrients include water, a source of energy, nitrogen, vitamins, and minerals.
Microorganisms select their food as substrate on the basis kinds and proportions of nutrients present in foods.
Introduction
Factors Responsible for Food Spoilage
Chemical Changes due to Spoilage
Spoilage of Different Foods
Spoilage of Meat
Spoilage of Poultry and Poultry Products
Spoilage of Fish and other Sea Foods
Spoilage of Fruits and Vegetables
Spoilage of Cereals and Cereal Products
Spoilage of Milk and Milk Products
Spoilage of Soft Drinks, Fruit Juices, Fruit Preserves
Miscellaneous Products
Microbial spoilage of fruits, vegetables, cereals, meat, sea foods, soft drinks, canned foods
Introduction
Food spoilage results when microbiological, chemical, or physical changes occur,
rendering the food product unacceptable to the consumer. Microbiological food
spoilage is caused by the growth of microorganisms which produce
enzymes that lead to objectionable by-products in the food.
The numerous sources of microbial spoilage come from undesired yet ubiquitous
micro-organisms which can originate from the natural habitat, e.g. soil, water, air,
spoiled raw materials, biofilms on the surface of equipment, personal hygiene of
food workers.
The predominant bacteria associated with spoilage are Brochothrix
thermosphacta, Carnobacterium spp., Lactobacillus spp., Lactococcus spp.,
Leuconostoc spp., Pediococcus spp., Stretococcus spp., Kurthia zopfii, and
Weisella spp.
Spoilage bacteria are microorganisms too small to be seen without a
microscope that cause food to deteriorate and develop unpleasant odors,
tastes, and textures. These one-celled microorganisms can cause fruits and
vegetables to get mushy or slimy, or meat to develop a bad odor.
Microbial spoilage -Factors Responsible for Food Spoilage
Introduction
Factors Responsible for Food Spoilage
1. Various factors cause food spoilage, making items unsuitable for consumption.
2. Air and Oxygen -One important cause of food spoilage and spoilage is air and oxygen. Because air is colorless,
odorless, and tasteless, it is often taken for granted and sometimes forgotten as a means to cause food to spoil.
Microorganisms -Oxygen can provide conditions that enhance the growth of microorganisms. Some bacteria require
oxygen for growth (aerobes) while others can grow only in the absence of oxygen (anaerobes). Many bacteria can grow
under either condition and are called facultative anaerobes.
Enzymes -Certain enzymes that are naturally present in food are known as oxidizing enzymes. These enzymes
catalyze (speed up) chemical reactions between oxygen and food components, and this leads to food spoilage.
Oxygen -Oxygen can also cause spoilage of foods spontaneously, by itself. Oxidative spoilage is the chief cause of
quality loss in fats and fatty portions of foods. When lipids oxidize, short chain carbon compounds are formed; these
compounds have very strong odors and flavors and are very undesirable and unacceptable. The off -odors resulting
from this type of spoilage are sharp and acrid and have been described as linseed oil-like, tallowy, fishy or perfume-like.
Moisture -Water is one of the most common substances on earth. It is an essential component of all foods. The amount
of water in food (known as percent water) influences the appearance, texture, and flavor of the food. All living
organisms, as well as food, contain water.
Perishability of Food Classification
1. The chemical changes that occur in a food due to spoilage depend primarily upon the composition of food. The changes
vary depending upon the source of the food i.e. plant foods are primarily carbohydrate-rich, while animal foods are rich in
proteins and fats, hence the changes will differ. Let us see what changes takes place withrespect to the nutrients present
in the food when bacteria acts upon them.
2. Degradation of Carbohydrates The carbohydrates can be divided into monosaccharides, disaccharides, oligosaccharides
and polysaccharides. Most commonly occurring carbohydrates are disaccharides and polysaccharides. Bacteria
breakdown these carbohydrates to monosaccharides. This results in softening or liquefaction of the food.
3. Degradation of Fats Fats are the esters of glycerol and fatty acids and are called as 'glycerides'. Fats are susceptable to
hydrolysis, oxidation and other chemical changes that produce both desirable and undesirable flavour changes in foods.
The pure fat cannot be attacked by microorganisms. They need an aqueous phase to grow in foods like butter,
creamsand margarine. Microorganisms degrade fats into glycerol, free fatty acids, ketones and alcohols.
4. Degradation of Proteins Proteins are composed of amino acids combined by peptide linkages. The native proteins are
resistant to attack by microorganisms. The,other compounds like dipeptides and free amino acids in fi-esh meat, fish and
poultry are readily used by microorganisms.
5. Spoilage of the protein-rich foods may be evident before any significant amount of protein is degraded. The degradation
of amino acids is of primary importance in the spoilage of protein foods.
Spoilage of Different foods
Food spoilage results when microbiological, chemical, or physical changes occur, rendering the
food product unacceptable to the consumer. Microbiological food spoilage is caused by the growth of
microorganisms which produce enzymes that lead to objectionable by-products in the food
Different food behaves differently based on above factors and contents /nutrients present in the food
For instance, microorganisms that break down fats grow in sweet butter (unsalted butter) and cause a
type of spoilage called rancidity.
Another example occurs in meat, which is primarily protein. Bacteria able to digest protein (proteolytic
bacteria) break down the protein in meat and release odoriferous products such as putrescine and
cadaverine.
Food spoilage can also result in a sour taste. If milk is kept too long, for example, it will sour. In this
case, bacteria that have survived pasteurization grow in the milk and produce acid from the
carbohydrate lactose in it.
Meats and fish products are contaminated by bacteria from the animal's internal organs, skin, and feet.
Spoilage of Meat
Meat is primarily the muscular tissue which is edible portion of carcass of animals.
It contains all essential nutrients in the ready form for microbial growth and also it has higher water
content ( aw =0.99) which is ideal for most of microorganisms, particularly bacteria.
Spoilage of meat depends mostly on initial contamination of the animal – either during raising of
animal or its processing or preservation.
1. Initial Microflora – Hyginic condition of the animal are important for reduction of proliferation of
microorganisms in stored meat. E.g Sanitation of abattoir
2. pH – After the animal is slaughtered, the pH of the carcass srops from around 7.0 to 5.6, as the
glycogenreserve in muscles is used up slowly and lactic acid is formed.Condition of Animal ( e.g
stress/excitement) also abnormal reduction of glycogen.
3. Oxygen reduction potential – After slaughter, the oxygen stored in muscles gets reduced bringing
down the oxidation reduction potential. This condition is favorable to aerobic bacteria ( surface), while
internal condition still favors anerobic.
4. Bacteria’s/moulds causing Protein decomposition are most impacting in this case
5. Temperature – temperature if hold above 200C is subjected to anerobic bacteria. Once meat is
minced, both anerobic/aerobic can grow.
Spoilage of Poultry
Physical preservation procedures are mostly aimed at prolonging the durability of foods
by slowing down or repressing the spoilage mechanisms by the alteration of relevant
parameters.
Pickling – Fermentation of product in presence of of Salt/oil application with or
without heat
Use of Ionising or non ionizing radiations - They are ionizing radiations such as cathode
and gamma rays and non-ionizing radiation such as infrared and UV rays.
High pressure Processing -(HPP) is a “non-thermal” technique for food preservation
that efficiently inactivates the vegetative microorganisms
Pulse electric field processing - Pulsed light (PL) is used for the rapid inactivation of
microorganisms. The use of inert-gas flash lamps generating intense and short pulses
of ultraviolet (UV) light for microbial inactivation
Microwave processing - the use of electromagnetic waves of certain frequencies to
generate heat in a material. Because it is an electrothermal process, microbial
destruction by microwaves occurs through heat.
Food preservation : physical methods;
Refrigeration and
Dehydration
freezing
Controlled
Modified atmosphere
atmosphere Storage
packaging
Microwave processing
Use of Ionising or non
ionizing radiations
chemical preservation
There are many preservation techniques using chemical preservation.
1. Chemical preservatives –It Involves adding specific ingredients to foods and
food packaging that allows the food to remain safe and fresh. Preservative, in
foods are used to prevent or retard spoilage caused by chemical changes e.g
oxidation or the growth of mold. Along with other, preservatives also help to
maintain freshness of appearance and consistency. Majorly 3 class are used –
Benzoates, Nitrites and sulphates. However due to their side effects / rampant
use has caused concern for customer health. There are limits set up by many
Governments and even putting restrictions. Trend is increasing to avoid
chemical preservative as much as possible –called as Clean label.
2. Natural anti-microbiological agents -Antimicrobial components in plant
materials are commonly found in herbs and spices (rosemary, sage, basil,
oregano, thyme, cardamom, and clove), fruits and vegetables (guava,
pepper, cabbage, garlic, and onion, citrus), seeds and leaves (grape seeds,
fennel, nutmeg, parsley, and olive leaves)
3. Biology based preservation system -Bio-preservation is a technique of
extending the shelf life of food by using natural or controlled microbiota or
antimicrobials. The fermentation products as well as beneficial bacteria are
generally selected in this process to control spoilage and render pathogen
inactive. Bacteriocins, bacteriophages and bacteriophage-
encoded enzymes fall in this theory. The long and traditional role of Lactic acid
bacteria on food and feed fermentations is the main factor related to the use of
bacteriocins in bio-preservation.
Industrial Microbiology: Fermentation processes: the range, components and types
❑ The term “Fermentation” is derived from Latin Verb “Fervere” means to boil.
❑ Originally fermentation referred to the bubbling, observed when sugar or starchy material caveat
into alcoholic beverages. Here the bubbling is observed due to the production of Co2 gas.
❑ Later on the term “fermentation” was applied to the process in which alcohol was formed from
sugar.
❑ Later on Pasteur described fermentation as those anaerobic process through which microorganism
obtained energy for growth in the absence of oxygen. But, today Fermentation is broadly use for
both, aerobic & anaerobic metabolic activity of microorganism in which specific chemical changes
are brought in an organic substrate.
❑ Fermentation process normally is carried out either in presence of a fermenter which is majorly is
an Yeast or mold
❑ They need specific minerals or conditions to be present E.g Zinc is required for fermentation with
yeast.
❑ pH conditions are critical as inappropriate pH will not allow right yeast species to grow and
sometimes unwanted/risky strains might get overpowered and result into a damaged/or improper
fermentation.
❑ Presence of sugar as source of carbohydrate is must
Industrial Microbiology: Fermentation processes: the range, components and types