Biotechnology: Quarter 4 - Module 3: Biotechnology in Industry and Environment
Biotechnology: Quarter 4 - Module 3: Biotechnology in Industry and Environment
Biotechnology: Quarter 4 - Module 3: Biotechnology in Industry and Environment
Biotechnology
Quarter 4 – Module 3:
Biotechnology in Industry and
Environment
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Biotechnology
Quarter 4 – Module 3:
Biotechnology in Industry and
Environment
Introductory Message
This Self-Learning Module (SLM) is prepared so that you, our dear learners,
can continue your studies and learn while at home. Activities, questions, directions,
exercises, and discussions are carefully stated for you to understand each lesson.
Each SLM is composed of different parts. Each part shall guide you step-by-
step as you discover and understand the lesson prepared for you.
Pre-tests are provided to measure your prior knowledge on lessons in each
SLM. This will tell you if you need to proceed on completing this module or if you
need to ask your facilitator or your teacher’s assistance for better understanding of
the lesson. At the end of each module, you need to answer the post-test to self-check
your learning. Answer keys are provided for each activity and test. We trust that you
will be honest in using these.
In addition to the material in the main text, Notes to the Teacher are also
provided to our facilitators and parents for strategies and reminders on how they can
best help you on your home-based learning.
Please use this module with care. Do not put unnecessary marks on any part
of this SLM. Use a separate sheet of paper in answering the exercises and tests. And
read the instructions carefully before performing each task.
If you have any questions in using this SLM or any difficulty in answering the
tasks in this module, do not hesitate to consult your teacher or facilitator.
Thank you.
What I Need to Know
This module was designed and written with you in mind. It is here to help you
master the nature of Biotechnology. The scope of this module permits it to be used
in many different learning situations. The language used recognizes the diverse
vocabulary level of students. The lessons are arranged to follow the standard
sequence of the course. But the order in which you read them can be changed to
correspond with the textbook you are now using.
These objectives are aligned to learning competency for week 5-6 which is cite
application of Biotechnology in Industry and Environment.
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What I Know
Directions: Read each question carefully. Choose the letter of the correct answer.
1. It is where the worst oil spill in the country took place.
a. Bulacan c. Guimaras
b. Panay d. Pampanga
2. These are proteins involved in controlling and coordinating reactions that happen
simultaneously or consecutively.
a. Bacteria c. Biomass
b. Enzymes d. Microbes
3. The method of bioremediation which injects a small number of oil-degrading
microbes into an affected area.
a. Bioaugmentation c. Biomass
b. Biosensor d. Biostimulation
4. The addition of nutrients to stimulate the growth of innate oil-degrading microbes
to increase the rate of remediation?
a. Bioaugmentation c. Biomass
b. Biosensor d. Biostimulation
5. It is a type of bioremediation that you allow to take place while leaving the soil or
water in its natural location.
a. Biomass c. Biosensor
b. Ex-situ d. In-situ
6. The device which made up of a transducer and a biological element that may be
an enzyme, an antibody or a nucleic acid.
a. Biomass c. Biosensor
b. Ex-situ d. In-situ
7. It is a type of bioremediation would scoop up the contaminated soil or pump up
the contaminated water and take it somewhere else for bioremediation.
a. Biomass c. Biosensor
b. Ex-situ d. In-situ
8. The following are steps of In-situ EXCEPT:
a. Recovery of free product c. Site investigation
b. Scoop up the contaminated soil d. Treatability studies
9. These are organisms that utilize chemical contaminants in the soil as an energy
source.
a. Bacteria c. Microbes
b. Fungi d. Parasites
10. It is the other term for Bioremediation.
a. Biomass c. Biosensor
b. Biostimulation d. Bio treatment
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Lesson
Biotechnology in Industry
1 and Environment
What’s In
ACTIVITY A
Directions: Read the questions carefully and choose the correct answer in the table
below.
Biotechnology GM Papaya Bt Corn Genes Bt Cotton
in Agriculture
Flavor Savor GM Peas Sugar Beets GMO Golden Rice
1. This refers to the organisms whose genes have been modified by genetic
manipulation.
2. It is the first commercially grown GM tomato granted with a license for human
consumption.
3. The first genetically modified organism with the insertion of toxin gene from the
bacterium Bacillus thuringiensis.
4. The organisms were engineered to grow faster and to be more resistant to weeds.
5. It helps in modifying plants, animals, and microorganisms and improves their
agricultural productivity.
6. The genetically modified organism containing beta carotene, a precursor of vitamin
A.
7. The GMO which developed to resist infection by ringspot virus.
8. It was created by inserting kidney beans genes into another DNA resulting in the
production of a protein with pesticidal ability.
9. The part of the organism which has been altered using genetic engineering
techniques.
10. The genetically modified crops which is produced to resist pests and pesticides.
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What’s New
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What is It
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2. The cleanup of contaminated soil - Human activity has introduced many
toxic substances into the environment’s soil and groundwater. During
bioremediation, microbes utilize chemical contaminants in the soil as an
energy source and, through oxidation-reduction reactions, metabolize the
target contaminant into useable energy for microbes.
3. Oil spill cleanup – there are instances in our country that oil spill happens
like in the Guimaras oil spill occurred in the Panay Gulf on August 11, 2006,
when the oil tanker MT Solar 1 sank off the coast
of Guimaras and Negros islands in the Philippines, causing what is
considered as the worst oil spill in the country. Due to the effectiveness and
lower cost of bioremediation, two methods were used to clean up an oil spill.
• Bioaugmentation which is the injection of a small number of oil-degrading
microbes into an affected area.
• Biostimulation which is the addition of nutrients to stimulate the growth of
innate oil-degrading microbes to increase the rate of remediation.
Fungi
Plant
s
Green Earth
Bacteria
Photo source: jagranjosh.com
In-situ and Ex-situ tell us something about where the bioremediation is taking
place. In-situ really just means in place or on-site (keeping something in its natural
place), and ex-situ just means out of place or off-site (removing something from its
natural place). In-situ bioremediation means that you allow bioremediation to take
place while leaving the soil or water in its natural location. The steps of in situ
bioremediation are site investigation, treatability studies, recovery of free product
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and removal of the contamination source, design and implementation of the in-situ
bioremediation system, and lastly monitoring and performance evaluation of the in-
situ bioremediation system. And, Ex-situ bioremediation means you would scoop up
the contaminated soil or pump up the contaminated water and take it somewhere
else for bioremediation (Hartsock, 2016).
NO Na Na
Na 2 +
Na Na
Na Mg
Na
Na
Soil Mg Soil
particle particle
The term “biosensor” is short for “biological sensor.” The device is made up of a
transducer and a biological element that may be an enzyme, an antibody or a nucleic
acid. The bioelement interacts with the analyte being tested and the biological
response is converted into an electrical signal by the transducer. Depending on their
particular application, biosensors are also known as immunosensors, obtrudes,
resonant mirrors, chemical canaries, biochips, glucometers, and biocomputers. It is
then possible to make quantitative measurements of pollutants with extreme
precision or to very high sensitivities. The biological response of the biosensor is
determined by the biocatalytic membrane, which accomplishes the conversion of
reactant to product.
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What’s More
Activity 1
Direction: Complete the Fact or Bluff table below by sorting the statements on
Table A.
FACT BLUFF
TABLE A
Bio remediators can be naturally Microorganisms have been used for many
evolved or genetically engineered. years to remove organic matter and toxic
chemicals from domestic and
manufacturing waste discharge.
Guide Questions:
1. What are the other terms for Bioremediation?
2. How does Bioaugmentation differ from Biostimulation?
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Activity 2
Directions: Using a Venn diagram, write the differences between In-situ and Ex-
Situ. In the middle, write their similarities.
In-Situ Ex-Situ
Guide Questions:
1. What is the role of microbes in Bioremediation?
2. How beneficial is Bioremediation in environmental clean-up?
Activity 3
Directions: Use all the syllables in the syllabox to identify the terms and methods in
the Applications of Biotechnology in Industry & Environment. The number of letters
and syllables are given as clues.
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it somewhere else for bioremediation.
8. _ _ _ _ _ _ _ _ _ _ _ _ _ _ (6) It is used for the production of food supplements
such as vitamin B2, bio-based plastics.
9. _ _ _ _ _ _ _ _ (2) It utilizes chemical contaminants in the soil as an
energy source.
10. _ _ _ _ _ _ _ _ (3) It is the place where the worst oil spill happened in
the country.
Guide Questions:
1. What is Biosensor and how does it work?
2. How microorganisms balance our environment?
Activity 4:
Directions: Biotechnology has so many uses in different fields. Enumerate the
applications of biotechnology based on what you learned/read. Write your ideas in
the concept webbing below:
Guide Questions:
1. How would you find Biotechnology useful in industry and the environment?
2. What is the best use of Biotechnology in the concept web you made?
3. What other applications can you suggest to maximize the use of biotechnology?
Activity 5
Directions: Unscramble the letters to identify the terminologies used in the
applications of Biotechnology
SIOM ABS 1. The material produced by or remaining after the death of organisms
(e.g., bacteria, plants, and animals). A collection of technologies that use living cells
and/or biological molecules to solve problems and make useful products.
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DIET NO MORE BADI 2. The use of organisms, usually microorganisms, to break
down pollutants in soil, air, or groundwater.
Guide Question:
1. How does bioaugmentation work? And give an example of bioaugmentation in
our country.
Activity 6.
Direction: Arrange the steps in In-situ Bioremediation below. Use numbers 1-5 to
label the process.
No. Steps
Treatability studies.
Design and implementation of the In-situ bioremediation system.
Site investigation.
Monitoring and performance evaluation of the In-situ bioremediation system.
Recovery of free product and removal of the contamination source.
Guide Questions:
1. What is In-Situ method?
2. Which is better In Situ or the Ex-Situ? Explain your answer.
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What I Have Learned
Let’s sum up what you have learned. Complete the sentences below.
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What I Can Do
Directions: Make a simple dictionary of words that you find hard to understand
which are commonly used in the world of Biotechnology in Industry and
Environment. Follow concrete procedures below:
1. Make personalize and hand-written dictionary using long bond paper (Half folded)
2. Include local and foreign terminologies which are used in biotechnology in
industry and environment.
3. Make at least 3 pages.
4. Bind and cover it.
5. Submit after 2 weeks together with the other answers on this module.
6. Your output will be rated using the scoring rubric
CRITERIA SCORE
Done creatively and neatly with more than 3 pages 10
Done creatively and neat enough with 2 pages 7
Done creatively and neat enough but with 1 page 5
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Assessment
Multiple Choice. Choose the letter of the best answer. Write the chosen letter on a
separate sheet of paper.
1. What are these proteins involved in controlling and coordinating reactions that
happen simultaneously or consecutively?
a. Bacteria c. Enzymes
b. Biomass d. Microbes
2. Which process uses biological organisms to break down hazardous
substances into less toxic or nontoxic substances?
a. Bioaugmentation c. Biosensor
b. Bioremediation d. Biostimulation
3. When did the Guimaras oil spill occur?
a. August 11, 2006 c. September 11, 2006
b. August 11, 2007 d. September 11, 2007
4. Which method will add nutrients to stimulate the growth of innate oil-
degrading microbes to increase the rate of remediation?
a. Bioaugmentation c. Biosensor
b. Bioremediation d. Biostimulation
5. What device is made up of a transducer and a biological element that may be
an enzyme, an antibody or a nucleic acid?
a. Bioaugmentation c. Biosensor
b. Bioremediation d. Biostimulation
Directions: Write True if the statement is correct and False if incorrect. Then change
the underlined word/s to make the statement correct.
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Additional Activity
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What I Know What’s More
1. C 6. C
ACTIVITY 1
.
2. B 7. B
3. A 8. B Fact
4. D 9. C - Bioremediation predominantly utilizes bacteria,
5. D 10. D archaea, and fungi.
-Bioremediation can breakdown nearly 100% of
compounds found in petroleum spills.
-Using organisms to breakdown and degrade
What’s In pollutants
1.GMO -Adding engineered organisms to a system for breaking
2. Flavor Savor down pollutants
3. Bt Corn
-Bioremediators can be naturally evolved or genetically
4. Sugar Beets
engineered.
5. Biotechnology in Agriculture
6. Golden Rice -Bio treatment, bio reclamation and bio restoration are
7. GM Papaya the other terminologies for bioremediation.
8. GM Peas
9. Genes -Microorganisms have been used for many years to
10. Bt Cotton remove organic matter and toxic chemicals from
domestic and manufacturing waste discharge.
Bluff
What’s New
1. IE -Bioremediation only works on naturally occurring
2. ND pollutants.
3. ND
-Bioremediation is collecting and storing pollutant.
4. ND
5. IE -Bioaugmentation is the addition of nutrients to
6. ND stimulate the growth of innate oil-degrading microbes
7. ND to increase the rate of remediation.
8. IE
9. IE Guide Quest ions
10. IE 1. Bio treatment
2. Biostimulation is a bioremediation process
designed to remove pollutants from an
environment. Bioaugmentation is a method
used in laboratories to promote the growth of
fastidious microbes.
Answer Key
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What’s More
Activity 2
In situ
-a method of breaking down and purifying hazardous substances present in soil and
groundwater.
Ex Situ
-techniques involving the transfer of a target species away from its native habitat to a place
of safety, such as a zoological garden, botanical garden, or seed bank.
Similarities
-Use of microorganisms to remove or detoxify pollutants.
Guide Quest ion
1.they operate the basic drug cycles that are necessary for the plants' supply of nutrients
via the reaction of organic matter in the soil.
2. Bioremediation works by providing these pollution-eating organisms with fertilizer,
oxygen, and other conditions that encourage their rapid growth.
What’s More
Activity 3
1. Enzymes
2. Bioremediation
3. Bioaugmentation
4. In Situ
5. Biostimulation
6. Biosensor
7. Ex Situ
8. Microorganisms
9. Microbes
10. Guimaras
Guide Quest ions
1. A biosensor is a device that measures biological or chemical reactions by generating
signals proportional to the concentration of an analyte in the reaction.
2. They use carbon dioxide and release oxygen during photosynthesis. In this way, the
oxygen and carbon dioxide balance in the environment is maintained. Saprophytic bacteria
breakdown dead organic matter into inorganic substances that can be recycled in the soil to
make the soil more fertile.
Activity 4
1. Bioremediation
2. Bioaugmentation
3. Biostimulation
4. Biomass
5. Biosensor
6. In situ
7. Ex situ
Guide quest ions
1. Industrial biotechnology enables a cleaner, more energy-efficient production of
consumable goods, utilizing renewable raw materials instead of fossil resources.
2. Bioremediation works by providing these pollution-eating organisms with fertilizer,
oxygen, and other conditions that encourage their rapid growth. These organisms
would then be able to break down the organic pollutant at a correspondingly faster
rate. In fact, bioremediation is often used to help clean up oil spills.
3. Bio-based sustainable production of chemicals, energy, fuels, and materials.
Engineering sustainable food production
Sea-water based bio-processes.
Non-resource draining zero waste bio-processing.
Using carbon dioxide as a raw material.
Regenerative medicine.
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What’s More
Act ivity 5
1. Biomass
2. Bioremediation
3. Enzymes
4. Industrial
5. Bioaugmentation
Guide Question
Ø Bioaugmentation is used to biodegrade specific soil and groundwater contaminants. It
involves adding cultured microorganisms into the subsurface to biodegrade the desired
contaminants. In many cases, these microorganisms are “specialists” in degrading
specific target contaminants. Example due to its many favorable properties, water
hyacinth can be used as feedstock for generating biodiesel.
Act ivity 6
1. 2
2. 5
3. 1
4. 4
5. 3
Guide Quest ions
1. In situ typically refers to a method of data collection or manipulation of a sample without
exposure to an external environment.
2. In situ conservation offers greater mobility to the animal species being safeguarded in its
habitat. Ex situ conservation provides less mobility to the organisms due to relatively smaller
habitat or area than the in situ.
What I Have Learned Assessment
1. White Biotechnology 1. c
2. Environment biotechnology 2. B
3. Bioremediation 3. A
4. Crime scene clean up 4. d
5. The cleanup of contaminated soil 5. c
TRUE OR FALLS
6. Oil spill clean up
1. True
7. Bioaugmentation
2. Biostimulation
8. Biostimulation 3. Environmental
9. In situ 4. True
10. Ex situ 5. In situ
Additional Activity
Ø The answer may vary.
What I Can Do
>The student will make a simple
dictionary of words that they find it
hard to understand which are
commonly used in the world of
biotechnology in Industry and
Environment.
References
In Situ Bioremediation: Definition & Techniques. (2016, June 17). Retrieved from
https://study.com/academy/lesson/in-situ-bioremediation-definition-
techniques.html
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