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CC1 Laboratory Examination

The document provides information about various laboratory equipment and procedures. It includes descriptions of common lab equipment like mortar and pestle, test tubes, pipettes, and slides. It also covers topics like order of blood draw, fire safety classifications, chemical waste handling, and differentiating between lab tools. Multiple choice, matching, and short answer questions are asked to test understanding of laboratory concepts and procedures.

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Greg Amor Omapas
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278 views5 pages

CC1 Laboratory Examination

The document provides information about various laboratory equipment and procedures. It includes descriptions of common lab equipment like mortar and pestle, test tubes, pipettes, and slides. It also covers topics like order of blood draw, fire safety classifications, chemical waste handling, and differentiating between lab tools. Multiple choice, matching, and short answer questions are asked to test understanding of laboratory concepts and procedures.

Uploaded by

Greg Amor Omapas
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOCX, PDF, TXT or read online on Scribd
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Glory Blessie A.

Omapas MLS 2B

LABORATORY EXAMINATION

1. Identify the following apparatus and give its corresponding function. Information required beside
some of the pictures are additional answer that should be given. 2pts each.

a. Mortar and Pestle – is both used to crush and grind solid substances. Mortar is
the bowl where the substances are being crushed, while, the pestle is the bat-
shaped object used fpr crushing, grinding, and mixing. This apparatus can be made
from wood, ceramic, or stone.

b. Test Tube Clamp – is used to hold the test tube when the tube contains
hazardous or hot chemicals.

Test tube – where substances, mostly liquid is contained and tested in many
ways like being burned over the Bunsen burner or centrifuged.

c. Florence Flask – is used to contain liquid for uniform heating, distillation,


boiling, and easy swirling.

d. Serologic pipet – contain graduation marks to the tip and is a blowout pipet to deliver the full
vollume of the pipette. It is used to transfer milliliter volumes of liquid.

e. Mohr pipet – used to measure the volume of liquid


delivered and is a self-draining pipet in where the tip
should not touch the vessel while draining.
f. Ostwald-folin – is a blowolut pipet and is used for biologic fluid that possess
viscocity greater than water.

g. Micropipette – is commonly used to transfer volumes of liquid accurately and


precisely in microliter range. In terms of accuracy, the micropipette has higher
accuracy comparedto the glassware pipets as they can measure up to 0.1 micro
that is highly demanded in experimental activities. Another advantage of using
micropipette rather than glassware pipet is the comfort of use because of the
rise of ergonomically sustainable hand-held pipettes.

h. Glass slide – used as a platform to contain specimen for microscopic


observation.

i. Pasteur pipette (glass) – do not have calibration marks and are


used to transfer solutions and liquids irrespective of specific
volume.

MATCHING TYPE:
Tubes and additives.

COLUMN A COLUMN B

___e___1. Lavender top a. no inversion with glass

___d___ 2. Royal Blue top b. complement inactivation

___f___ 3. Green top c. prevent glycolysis

___c___ 4. Gray top d. trace element

___a___ 5. Red top e. compatibility testing

___b___ 6. Yellow top f. sodium heparin

g. gel and lithium heparin

h. cause bluish background

Classification of fires

___b___7. Class A a. centrifuge

___c___ 8. Class B b. fabric

___a___ 9. Class C c. gasoline

___d___ 10. Class D d. potassium

Fill in the blanks.

1. the fire triangle has been modified into a three-dimensional pyramid known as fire tetrahedron.

2. what hazard is associated with tenosynovitis? Ergonomic Hazard.

3. Compresses gases is one of the most widely used liquified gases in the lab.

4. what hazard does centrifuge belong? Mechanical Hazard.

5. what chemical waste is used as preservative that forms explosive salts with metals in pipes? Sodium
azide.

6. chemical wastes are subject to strict cradle to grave tracking under the RCRA.

7. what is the flash point of combustible liquids in Fahrenheit? 100°F.

8. eyewash stations in the laboratory must be within 100 feet.

9. respirators with high-efficiency particulate air (HEPA) filters must be worn when working directly
with patients with TB.
10. what is the concentration of bleach used to disinfect spillage of infectious material? 10%.

DISCUSSION:

1. DIFFERENTIATE THE FOLLOWING: 3pts each

a. serum and plasma – the main difference between the two is that the serum does not contain
fibrinogen while the plasma have fibrinogen that activates the blood clotting factors forming blood clot.
Therefore, plasma is the liquid portion of unclotted blood, while, serum is the liquid portion of the
clotted blood.

b. WBC and RBC thoma pipet – WBC thoma pipet is smaller than RBC pipet to dilute blood with fluid by
1:20 and 1:200 respectively.

c. serologic pipet and volumetric pipet – serologic pipet contains graduation marks to the tip and is a
blowout type of pipet, while, the volumetric pipet dispense and transfer aqueous solution in a self-
draining manner and has the greatest degree of accuracy and precision in terms of diluting standards,
calibrators, and quality-control material.

2. CASE STUDY: 10 PTS

a. A PHLEBOTOMIST MUST COLLECT A CBC SPX FROM A 3-YR OLD BOY.

 WHAT SITE SHOULD BE CHOSEN?

The site for the toddler’s venipuncture would be the antecubital vein of the arm. With
assistance from the parent, sufficient blood specimen should be drawn for the CBC test.

b. A PHLEBOTOMIST MUST PERFORM A VENIPUNCTURE TO COLLECT RED,LIGHT BLUE,GREEN,AND


LAVENDER.

 WHAT IS THE ORDER OF DRAW?

The correct order of draw would be Light Blue, Red, Green, and Lavender.

TUBES ADDITIVE ASSIGNED LABORATORY


DEPARTMENT
Light Blue Sodium citrate Hematology Department;
Chemistry Department
Red None (glass); clot activator Immunology and Serology
(plastic) Department; Blood bank
Department; Chemistry
Department
Green Sodium heparin or lithium Chemistry Department;
heparin Hematology Department
Lavender Ethylenediamine tetraacetic Hematology Department; Blood
acid (EDTA) bank Department

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