DXH Series Training Pocket Resources Spiralbook Rev A June2014

UniCel DxH Series
Coulter Cellular Analysis System

Training Pocket Resources
This document is not intended to replace the information in your Instrument Instructions for
Use Manual (IFU). Information in the Instructions for Use Manual supersedes information in any other manual.
UniCel DxH Series Training Pocket Resources

Rev. A (June 2014) Beckman Coulter Professional Development
UNICEL DXH SERIES COULTER CELLULAR ANALYSIS SYSTEM TRAINING POCKET RESOURCES
WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE
ATTEMPTING TO OPERATE INSTRUMENT.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR
MAINTAINING THIS OR ANY OTHER AUTOMATED LABORATORY EQUIPMENT.
INTENTION FOR USE

This document is not intended to replace the information in your Instrument
Instructions for Use Manual (IFU). Information in the Instructions for Use Manual
supersedes information in any other manual.
TRADEMARKS
THE BECKMAN COULTER logo, COULTER, DxH, and UniCel are trademarks of
Beckman Coulter; the BECKMAN COULTER logo COULTER, and UniCel are registered
in the USPTO.
All other trademarks, service marks, products or services are trademarks or registered
trademarks of their respective holders.
Copyright  Beckman Coulter, Inc. 2014

All Rights Reserved
Find us on the World Wide Web at:

www.beckmancoulter.com
Beckman Coulter Ireland, Inc.

Mervue Business Park, Mervue Galway, Ireland 353 91 774068
Made in USA
ii of vi UniCel DxH Series Training Pocket Resources

Beckman Coulter Professional Development Rev. A (June 2014)
REVISION STATUS
Revision A (June 2014)

Software release 1.0
Presented June 2014, Miami, Florida
The information contained in this manual has been reviewed and

approved. The references listed below have been combined.
The materials are linked to the following references:
UniCel DxH Pocket Resources

Revision B (December 2013)
Presented March 2013, Miami, Florida
UniCel DxH 800 Training Resources

Revision A (March 2013)
UniCel DxH Slidemaker Stainer Training Resources

Revision A (March 2013)
UniCel DxH Series Training Pocket Resources iii of vi

iv of vi UniCel DxH Series Training Pocket Resources

UniCel DxH Series Coulter Cellular Analysis System Training Pocket Resources
TRAINING RESOURCES TOPICS
UNICEL DXH SERIES JOB AIDS
UNICEL DXH SERIES CLEANING PROCEDURE CHART
UNICEL DXH SERIES COMPETENCY CHECKLIST
EXAMPLES OF PATIENT RESULTS
TROUBLESHOOTING PROCEDURES
UNICEL DXH SLIDEMAKER STAINER JOB AIDS
UNICEL DXH SLIDEMAKER STAINER CLEANING PROCEDURE

CHART
UNICEL DXH SLIDEMAKER STAINER COMPETENCY CHECKLIST
STAIN PROTOCOLS
STEPS ON ACCESSING TRAINING MATERIALS ON THE WEB
UniCel DxH Series Training Pocket Resources v of vi

UniCel DxH Series Coulter Cellular Analysis System Training Pocket Resources
vi of vi UniCel DxH Series Training Pocket Resources

UniCel DxH 800/DxH 600 Coulter
Cellular Analysis System
Front View Specimen Processing Module (SPM)
Rev. A (June 2014) 1 of 118

Beckman Coulter Professional Development
System Overview
Rev. A (June 2014) 2 of 118

UniCel DxH 800 Configuration Only
Floor Stand
Rev. A (June 2014) 3 of 118

UniCel DxH 800/DxH 600 &
DxH Slidemaker Stainer
Log off / Log on
Step Action
To Log Off from the System Manager
1 Select the Log off icon at the top right of the screen.
On the Log off screen, select the Log off button.
3 You are now logged off from the System Manager.
To Log On to the System Manager
On the Log On screen, type in the Operator ID, press tab (case sensitive).
2 Type in the Password (case sensitive).
3 Select the Log On button or Press Enter.
4 You are now logged on to the System Manager.
Rev. A (June 2014) 4 of 118

Home Screen
5 6 7
2 8
4
1. Status Area 5. System Status Icon
2. Navigation Icons 6. Alert Status Icons
3. Local Navigation Icons 7. Utility Icons
4. Status Bar 8. System Icons
Rev. A (June 2014) 5 of 118

Access System Help
Step Action
1 Select the System HELP icon .
You may search for topics using:

2
“Content”, “Index” and “Search” tabs located on the left side of Help.
3 To place topic in Favorites as a reference, select the Favorites tab.
4 Locate a specific topic of interest.
5 Then, select the “Add” button at the bottom of the display.
6 You may now refer to this topic any time you select Favorites.
7 To delete a topic from Favorites, highlight it and select “Remove”.
The Help screen may be moved and resized to place it in a better position on the
8
screen while you are working.
The font icon at the top of the Help screen allows you to increase or decrease the size
9
of the font used to make it easier to read.
The print icon at the top of Help allows you to print any information that you want from
10
Help. It will print in the font size chosen.
11 To close Help, select “X”
Rev. A (June 2014) 6 of 118

System Status & Details Status Screens
Step Action
Select the System Status icon . This screen gives you an overview of the system.
System
Status
Transport Instrument
Status Status
1
LIS Printer
Status Status
Details
Status
Rev. A (June 2014) 7 of 118

Step Action
Click on Details Status button, or double click on the instrument silhouette to view
the systems status details.
Date & Time of last Daily Displays the Status of the Unit
Check & Shutdown Cycles
Date/Time of Last Control Run Date/Time of Last

Calibration Change
Default Test Order
Automatic: Specimen - Cassette
2 Manual: Specimen - Single-Tube
Transport Status
Transfers
Rev. A (June 2014) 8 of 118

Daily Checks
Step Action
Select the Daily Checks button to display the Daily Checks Summary screen with
results of the most recent Daily Checks.
Select Daily Checks from the local navigation bar. A dialog box displays the
2 following: Are you sure you want to start a Daily Checks procedure? Press OK to
continue.
3 Select OK to run Daily Checks, or Select Cancel.
The Daily Checks takes approx 4.5 minutes. For additional reference, refer to Daily
4
Checks Help topic Chapter 3.
Rev. A (June 2014) 9 of 118

Reviewing the Daily Checks
Step Action
Look at the Daily Checks alert status icon. If it is neutral, all is well. If it is red, then
something failed and needs review.
1
When there is any result that fails, you need to acknowledge the value to return the
status color to neutral to be able to run the instrument.
2 Look at each Daily Checks tab, beginning with the Summary tab.
3 If any Background result fails, run Daily Checks again.
4 Verify your background results when the Daily Checks cycle finishes.
You may view the Daily Checks log entries and any areas that failed within
5
the Daily Checks will be backlit in red and have a Fail status posted.
Rev. A (June 2014) 10 of 118

Shutdown
The DxH System allows you to program the length of time the instrument is in Shutdown.
Beckman Coulter recommends that the SPM remain in cleaner for at least 30 minutes every 24
hours. Shutdown can be initiated manually or automatically.
NOTE: If the instrument exceeds 24 hours without having a shutdown performed,
the DxH System Status (Details Status Screen) will have Date/Time Shutdown box
backlit in yellow. This serves as an alarm to the operator. No additional system
messages will appear in the History Log.
At the end of your programmed time, the system removes DxH Cleaner and replaces it with
DxH Diluent. It then awaits a Daily Checks cycle, performed manually or automatically.
If a Shutdown cycle needs to be interrupted, just cancel and then request a Daily Checks cycle.
Step Action
From the Daily Checks screen, select the Shutdown button to display the Manual
1
shutdown dialog box.
Select the Perform Startup After Shutdown check box to automatically begin Daily
3
Checks after shutdown is completed.
Type a number in the Time in Cleaner Hours and Minutes check boxes to determine
the time in cleaner.
4 Note: No other cycles or System Manager actions can be performed until the
Shutdown cycle has completed. Once the Shutdown cycle is complete, a countdown
timer bar appears at the bottom of the Daily checks screen to let you know how much
time remains for the cleaning.
5 Select OK to begin shutdown or Cancel to exit.
Rev. A (June 2014) 11 of 118

Quality Control
Step Action
The frequency of running controls is dependent upon regulatory and laboratory policy
for quality assurance programs.
Remove control tube(s) from the refrigerator and warm at AMBIENT TEMPERATURE
1
for 10-15 minutes.
After warming, mix by hand as follows:

a. Roll the tube slowly between the palms of the hands eight times in an upright
position.
b. Invert the tube and slowly roll it between the palms eight times.
2 c. Gently invert the tube eight times.
Repeat steps 2a to 2c for 6C Controls and Body Fluids Controls.

For Retic-X, Inpect the tube contents for complete resuspension. If necessary, repeat
3
steps 2a to 2c until tube contents are uniformly suspended.
Do Not Over Mix. DO NOT use any type of mechanical mixer.
4 To put the instrument Online, select the Start button from the upper right.
5 Process all the controls (except Body Fluid) via cassette presentation.
After the cassettes are finished, follow the steps for single-tube presentation to
6
process the Body Fluid controls.
7 Return the control tube(s) to the refrigerator within 30 minutes.
Rev. A (June 2014) 12 of 118

Reviewing Control Results
Step Action
1 Select the Quality Control status icon .
If any of the control runs had out-of-range values, a dialogue box opens with a list of
those controls. If everything was within range, the Quality Control (Data View) screen
opens with the last control that was viewed.
If you want to review a different control or level, use the Select Control button
from the local navigation bar.
2
This opens a control tree that you can expand to choose the various controls and
levels for review. Highlight the control you wish to view and select OK.
Find Summary Data (statistics) on the left of the screen.

Find the results of the most recent run to the right, the column highlighted blue.
Values that are outside of the expected range are in red.
3
At the bottom of the column, you will see the word Exclude. If you select this
checkbox, the run will not be included in the statistics. If you deselect the checkbox, it
will be included again.
Select the View Graph button from the local navigation bar. This allows
4
review of the Levey-Jennings graphs.
5 Return to the data screen by selecting the View Data button.
Select the Comment button from the local navigation bar.

6
You may add to or edit comments.
Select the “Reviewed By” area under the column to review controls. In your lab, follow
7
your laboratory’s protocols for reviweing control results.
Rev. A (June 2014) 13 of 118

Supplies
Step Action
The hand-held Bar-Code scanner allows you to automatically upload DxH Supply
1 information from the Setup Supplies dialog box.
Select the Supplies icon from the Alert Status Icons.
Select Setup to set up supplies.
With the Setup Supplies dialog box open, scan the bar-codes on the Beckman
3
Coulter reagent container and hit Enter. The supply information is updated.
Note For Diluent Reagent only, select Diluent 1 or Diluent 2.
4 Select OK to complete the setup, or Next to configure another supply.
Rev. A (June 2014) 14 of 118

Configure Diluent Containers
Step Action
You can configure the system to run with one diluent container, or two (dual) diluent
containers. The system will automatically switch between two containers if so
configured.
Select the Supplies icon > Configure Containers.
You may select either the Single Container or Dual Container option from the
2
Diluent drop-down list.
3 Select the OK button to save your selection.
Configure Low Level Condition

Step Action
The Configure Low Level option allows you to set a warning level to alert you when
1 a supply is running low based on the remaining percentage of that supply.
Select the Supplies icon > Configure Low Level.
Select a Percentage from the drop-down list.
Note: When the low level is triggered Supplies Alert Status Icon turns yellow. If both
supplies are out, the icon is red.
3 Select OK.
Rev. A (June 2014) 15 of 118

Running Patient Samples
Cassette Presentation (LIS Bidirectional Download to Pending)
Step Action
You may view the status of the SPM in the System Status Screen or the Details
1 Status Screen.
Place the SPM online to run samples by pressing the start button .
2 Ensure your specimens have been collected and stored properly.
Load the specimens into the cassette.
Place the cassettes into the input buffer to the right of the SPM. The SPM
automatically begins cycling the cassettes.
Rev. A (June 2014) 16 of 118

Single Tube Presentation
Step Action
1 Select the Single-tube presentation icon .
The Single-tube Presentation Dialog Box will come up.
Place the specimen on the bar-code reader platform of the Single-tube

Presentation Station with the bar code facing the SPM to allow the Single-Tube
Presentation Bar Code Reader to scan the specimen label.
You may also type or scan the specimen identifier. You need to place the curser
at the end of the last character of the Specimen ID and press enter.
3
4 Verify the Specimen Identifier and Test request. Follow the prompts on the screen.
5 Mix the specimen according to your laboratory standards.
6 Place the specimen into the correct Single-tube position.
To process a sample collected in a micro-collection device, use single-tube

Note
presentation and place the tube on the right-hand side of the cradle.
Rev. A (June 2014) 17 of 118

Running a Sample in the Predilute Function
Step Action
The predilute function is a fixed x5 dilution.

Obtain diluent from the instrument, using the Diluent Dispense, to use in making a
dilution of a previously run sample. You will need an empty tube to obtain the
diluent. Follow the steps below.
1 Select the Single-tube Presentation icon .
Select the Diluent Dispense button.
3 Follow the directions on the screen, using an empty tube.
4 Prepare your x5 dilution. (Example: 50uL of sample and 200uL of diluent).
Rev. A (June 2014) 18 of 118

Running Sample in Predilute Function (Cont.)
Step Action
5 If SPM timed out, select the Single-tube Presentation icon .
Scenario One Scenario Two

Specimen ID in the Pending List Specimen ID is Not in the Pending List
1. Identify the specimen and press Enter. 1. Identify the specimen and press Enter.
2. Press Edit Order button. 2. The Dialog Box comes up Test Order not Found
“Default Test Order Assigned”. Select OK.
3. Highlight the Selected Panel and click

Remove. Then, select the Available Panel 3. Select the Available Panel PREDIx5.
PREDIx5 and click Add. Click Add.
4. Press the Submit button. 4. Press the Submit button.
Rev. A (June 2014) 19 of 118

Step Action
6 Place the specimen into the correct Single-tube position.
7 Once the sample is aspirated and the tube is returned.
8 Select the button to finish.
Rev. A (June 2014) 20 of 118

Calibration
Step Action
NOTE Before you can start or restart the calibration process, the SPM must be offline.
Select Menu>QA>CBC Calibration
Select Calibration Setup on the CBC Calibration Screen to display the CBC
Calibration Setup dialog box.
The screen will have the results of the last calibration run that was performed. You
2
will be prompted by a dialog box which states: Existing Data will be deleted.
Select OK to continue.
Select OK.
3 Type “10” in the Number of Aspirations text box.
4 Select Cassette Presentation Mode from the drop-down list.
5 Select BCI from the Calibrator Type drop-down list.
Select New Control From Bar Code and use the handheld bar-code scanner to
scan the 2D bar code on the product insert or enter/select the following
information:
6
Lot#
Expiration Date
Reference Values
Rev. A (June 2014) 21 of 118

Step Action
7 Select OK and follow the screen prompts.
Place the calibrator in a cassette and place it in the input buffer.

8
Select OK.
Review the calibration results.

9 If the procedure indicates you need to calibrate, continue with the calibration
procedure.
10 Select the Finish button at the bottom of the screen.
If a parameter needed to be calibrated, perform calibration verification by running

11
the 6C controls.
Additional Information
When To Calibrate and When To Verify
When to Calibrate
You should calibrate your instrument:
 At installation.
 After replacement of any component that involves dilution
characteristics (such as the BSV) or the primary measurements
(such as the apertures).
 When advised to do so by your Beckman Coulter
Representative.
 If you fail verify calibration procedure.
When to Verify
You should verify the calibration of your instrument:
 As dictated by your laboratory procedures, local or national
regulations.
 When controls begin to show evidence of unusual trends.
 When controls exceed the manufacture’s defined acceptable
limits.
 If the average ambient room temperature changes more than
10F or 12F from the calibrating temperature.
Rev. A (June 2014) 22 of 118

Front Area
For Information Use Only.
Do Not Remove the Covers Unless You are Instructed by a Beckman Coulter
Front Representative.
Blood Probe
Detector Vacuum
Manifold Probe
Waste
Line
Hamilton
Syringe
BSV
Aspiration
Probe BSV
Drip Tray
Rear
Blood
Detector
Behind the Sample Aspiration Mode (SAM)
Bleach
Vacuum
Port
Regulator
Vacuum Vacuum
Regulator Regulator
Fitting #2 Fitting #1
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY STANDARDS SUCH
AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO, PROTECTIVE EYEWEAR, GLOVES AND
SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR ANY OTHER AUTOMATED LABORATORY
ANALYZER.
Rev. A (June 2014) 23 of 118

Front Area
Air Mix Temperature Control (AMTC) Module
Diff Retic Clearing

NRBC Mixing Mixing Retic Stain
Mixing Chamber Chamber Mixing
Chamber Chamber

Barcode
Camera
Right
Left
Cradle
Cradle
Rev. A (June 2014) 24 of 118

Right Side Area
FMI
Pumps
Sheath
Regulator
VCSn
Waste
Chamber
Sample
Regulator
Sample
Tank
Sheath
Tank
Solenoids
25 psi
Chamber
Rev. A (June 2014) 25 of 118

Left Side Area
FMI
Pumps
RBC
Bath
WBC
Count
Chamber
Hemoglobin
Lamp
RBC
Hemoglobin Count
Cuvette Chamber
WBC
Bath
Foam
Trap
Rev. A (June 2014) 26 of 118

Multi-Transducer Module (MTM)
Flow Cell
Rev. A (June 2014) 27 of 118

Cell Population
MONOS NEUTS
EOS
LYMPHS
BA
NON-WHITE CELLS
WBC’S
PLT/DEBRIS
WBC MATURE RBC’S
RETICS
NRBC
PLT/DEBRIS
Rev. A (June 2014) 28 of 118

All System messages are accompanied by “R” (Review) flags. Exceptions are the
System Messages associated with an Aspiration error (P Flag) and the Non-Blood
Specimen message (N Flag).
SYSTEM MESSAGE DXH SYSTEM MESSAGE DXH
WBC PLT
Abn WBC Pattern Platelet Clumps
Cellular Inter PLT Carryover
System Event: WBC PLT Inter: Debris
WBC Carryover RBC-PLT Overlap
RBC System Event: PLT
Abn RBC Pattern NRBC
System Event: RBC Abn NRBC Pattern
HGB AL2 Blank Volt: N
HGB Inter: WBC DataDisc: N
HGB Blank Shift High Event Rate: N
System Event: HGB Low AL2 Events: N
MCV Low DC Events: N
MCV Inter: PLT Low Event Rate: N
MCV Inter: WBC Low Events: N
DIFF NRBC Inter
Abn Diff Pattern NRBC-LY Overlap
Aged Sample System Event: N
Data Disc: D BF
Excessive Debris: D Abn TNC Pattern
High Event Rate: D System Event: TNC
High OP Events: D TNC Carryover
High RF Events: D P
Low Event Rate: D Bubbles
Low Events: D Carryover
Low OP Events: D No Aspiration
Low RMALS Events: D Non-blood Specimen
MO-NE Overlap Partial Aspiration
NE-EO Overlap Range Error
System Event: D Unknown Error
Undefined Pop: D ::::: Code
RETIC Flow Cell Clog: D
Abn Retic Pattern Flow Cell Clog: N
AL2 Blank Volt: R Flow Cell Clog: R
Data Disc: R
High Event: R
Low Event: R
Nucleated Cells
RET Inter: Debris
RET Inter: PLT
RET-RBC Overlap
System Event: R
Rev. A (June 2014) 29 of 118

RBC Histogram Examples
NORMAL
RBC AGGLUTININ MACROCYTIC, Elevated RDW
RBC Fragments / DI RBC / Elevated RDW DI RBCs, RBC Frags, Elevated RDW
Rev. A (June 2014) 30 of 118

WBC Histogram Examples
Lymphs Normal
Mononuclear Granulocytes
Left Shift
ImmGr Lymphocytosis Variant Lymph
Cellular Interference Eosinophilia Blasts
Rev. A (June 2014) 31 of 118

PLT Histogram Examples
Normal Small Platelets
Giant Platelets RBC / PLT Overlap
Rev. A (June 2014) 32 of 118

Better Abnormal Cell Detection
1
3 1 MO Blasts
4 2 NE Blasts
3 Immature Granulocytes
4 Band Neutrophils
5
5 LY Blasts
6 Variant Lymphocytes
6
7 Small Lymphs / Low Opacity
7 8 Non-White Cells
8
Rev. A (June 2014) 33 of 118

UniCel DxH 800/ DxH 600
Additional Information
This document is not intended to replace the information in your Instrument Instructions for Use Manual (IFU). Information
in the Instructions for Use Manual supersedes information in any other manual.
Event Log
All subcategories (System Status, Supplies, QC, QA, XB/XM, Patients, General)
Max of 5000 events, No limit on time, all instruments.
Data summary Logs

Supplies: Max of 500 events, No limit on time, per instrument.
Daily Checks: Max of 200 events, No limit on time, per instrument.
Audit Logs
Audit Log: Max of 30,000 events, 731 days, all instruments.
Maintenance Logs
All subcategories (Maintenance, Service): Max of 5000 events, No limit on time, per
instrument.
Specimen Preparation
The aspiration pump activates and aspirates 165 µL of sample (Cassette or
Single Tube Presentation). After the probe is removed from the specimen tube a
second pull of the aspiration pump draws the blood through the BSV pathway,
verifying a proper aspiration at the blood detectors.
WBC Dilution
~6.0 mL of DxH diluent
~28 µL of sample 1.251 Final dilution
~1.08 mL of DxH Cell Lyse
RBC Dilution
~10.0 mL of DxH diluent
~1.6 µL of sample 1.6250 Final dilution
AMTC (Air Mix and Temperature Control)

NRBC: 27 µL of sample
Diff: 34 µL of sample
Retic Stain: 29 µL of sample
Retic Clearing: 4 µL from the Retic Stain Chamber
Note: Probe discards ~4.0 µL of sample between each dispense in the AMTC.
Rev. A (June 2014) 34 of 118

UniCel DxH 800/DxH 600
Body Fluid Notes from DxH Instructions for Use Manual (IFU 629743AG Nov 2010)
This document is not intended to replace the information in your Instrument Instructions for Use
Manual (IFU). Information in the Instructions for Use Manual supersedes information in any other
manual.
Beckman Coulter recommends that a diluent be run as a Body Fluid sample prior to
analysis of Body Fluid specimens. Backgrounds within specifications can influence the
reported results on the samples with low abnormal or normal values. Beckman Coulter
recommends that each laboratory establish criteria for evaluation of the impact on the
background on the reported results.
Table 1.14 Background - Body Fluids*
* Analyzed by running a diluent using the Body Fluid Count cycle.

Table 1.11 Body Fluids (CSF, Serous, Synovial) Measuring and Operating Ranges
Body Fluids Reference Ranges

Reportable body fluid results obtained from the DxH System may exceed commonly
accepted normal reference ranges for all body fluids. Results should always be
interpreted in light of the total clinical presentation of the patient, including clinical
history, data from additional tests, and other appropriate information.
Cerebrospinal Fluid
The inability to collect cerebrospinal fluid specimen in the normal, non-diseased
population limits the ability to determine reference ranges. Literature (Kjeldsberg C,
Knight J. Body Fluids: Laboratory Examination of Cerebrospinal, Seminal, Serous &
Synovial Fluids. 3rd Ed., ASCP Press, Chicago, IL 1993.) suggests the following normal
reference ranges.
• WBC 0-5 cells/mm3 in adults
• WBC 0-30 cells/mm3 in children 1 to 4 years of age
• WBC 0-20 cells/mm3 in children 5 years of age to puberty
• RBC none to few
Rev. A (June 2014) 35 of 118

Serous Fluids
The accumulation of fluid in a serous cavity is an indication of a disease state. The
normal, non-diseased population has no fluid accumulation. Therefore, there are no
normal reference ranges for serous fluids. However, the number of cells present in a
serous fluid are used to aid in the classification, diagnosis and treatment of disease.
(Kjeldsberg C, Knight J. Body Fluids: Laboratory Examination of Cerebrospinal, Seminal,
Serous & Synovial Fluids. 3rd Ed., ASCP Press, Chicago, IL 1993.)
Synovial Fluid
The inability to collect synovial fluid specimens in the normal, nondiseased population
limits the ability to determine reference ranges. Literature suggests the following normal
reference ranges.
• WBC 0–150 cells/mm3

• RBC none
Sample Stability and Storage

Per established literature, Body Fluid samples should be stored at room temperature
and analyzed within 1 hour of collection. IMPORTANT Refer to CLSIH18-A3, Procedure
for handling and Processing of Blood Specimen for guidelines.
Overview
The following types of sample can be analyzed on the SPM.
• Anti-coagulated human whole blood in K2 or K3 EDTA

• Prediluted anti-coagulated human blood in K2 or K3 EDTA
• Human Cerebrospinal fluid (CSF)
• Human Synovial fluid in K2 or K3 EDTA or Heparin (pretreated with Hyaluronidase)
• Human Serous fluids in K2 or K3 EDTA
Body Fluids – (Pre-treating synovial fluids)

To reduce body fluid sample viscosity, use hyaluronidase to treat synovial fluids prior to
analysis according to your laboratory standards. Add in the ratio of 1 mL of synovial fluid
to 5 mg of hyaluronidase. Mix for 5 minutes.
Body Fluids – (Definition)

Fluids that are excreted, secreted, or derived form the human body. Some examples
are:
cerebrospinal fluid (brain and spinal cord), pericardial fluid (heart), peritoneal dialysis
fluid (abdomen), peritoneal lavage fluid (abdomen), peritoneal tap fluid (abdomen),
pleural fluid (lungs), and synovial fluid (joints).
Rev. A (June 2014) 36 of 118

Month: _______________ Year: __________
UniCel DxH Series Serial #:________________________________

Cleaning Procedures System ID:______________________________
DAILY 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Perform Shutdown
Perform Daily Checks - Verify that all results are
within limits
Process Controls
As Needed
Clean (Bleach) the Apertures
Clean the Aspiration Probe
Clean the BSV Externally
Clean the Pneumatic Supply Module Fan Filter
Clean the STM
Clean the Air Mix Temperature Control (AMTC)
Module
Clean the Vacuum Trap
Clean the Optical Sensors
Clean the Handheld Bar-Code Scanner
Tech Initials
Rev. A (June 2014) 37 of 118

Rev. A (June 2014) 38 of 118
Competency Checklist
UUNICEL
® DXH SERIES COULTER
® CELLULAR ANALYSIS SYSTEM
NICEL DXH 600 COULTER CELLULAR ANALYSIS SYSTEM
Achieved? Trainee Trainer
Task Resource How Measured? N/A Date
Y N Initial Initial
IDENTIFY POTENTIAL
HAZARDS AND SAFETY
FEATURES
Review MSDS www.beckmancoulter.co  Direct Observation
m  Test   
MSDS manual in lab  Other __________
Recognize Hazard Symbols System HELP  Direct Observation
 Test   
 Other __________
IDENTIFY AND BRIEFLY Training Manual  Direct Observation
EXPLAIN FUNCTION OF System HELP  Test   
ANALYZER COMPONENTS  Other __________
OVERVIEW OF SCREENS Training Manual
AND SOFTWARE System HELP
Identify System Manager  Direct Observation
Icons and Screens  Test   
 Other __________
Use System HELP  Direct Observation
 Test   
 Other __________
MANAGE REAGENTS AND Training Manual
CONSUMABLES System HELP
Setup new lot number for  Direct Observation
Latron CP-X, 6C, Retic-X  Test   
and Body Fluid Controls  Other __________
Replace reagent container  Direct Observation
 Test   
 Other __________
DAILY CHECKS AND Training Manual
SHUTDOWN PROCEDURES System HELP
Perform a Daily Checks Cycle  Direct Observation
& Review the Results  Test   
 Other __________
Rev. A (June 2014) 39 of 118

UUNICEL
Y N Initial Initial
Perform a Shutdown
 Direct Observation
 Test   
 Other __________
QA/QC Procedures Training Manual  Direct Observation

System HELP  Test   
 Other __________
Demonstrate proper handling Product Package Inserts  Direct Observation
of Latron CP-X, 6C, Retic-X  Test   
and Body Fluid Controls  Other __________
Run Latron CP-X, 6C, Retic-X Lab SOP  Direct Observation
and Body Fluid Controls  Test   
 Other __________
Review QC results per  Direct Observation
laboratory protocol  Test   
 Other __________
Download QC files for IQAP  Direct Observation
 Test   
 Other __________
Perform Calibration  Direct Observation
 Test   
 Other __________
Perform Repeatability and  Direct Observation
Carryover Procedures  Test   
 Other __________
PROCESS SAMPLES AND Training Manual
QUALITY CONTROL System HELP
MATERIALS Lab SOP
Load cassette with samples  Direct Observation
 Test   
 Other __________
Run control/sample using  Direct Observation
Cassette Presentation  Test   
with barcode ID and without  Other __________
Run control/sample using  Direct Observation
  
Single-tube Presentation  Test
Rev. A (June 2014) 40 of 118
UUNICEL
Y N Initial Initial
with barcode ID and without  Other __________
Retrieve Patient Results
 Test   
 Other __________
Demonstrate how to  Direct Observation

transmit/re-transmit results to  Test   
the LIS  Other __________
Reprint a Patient result from  Direct Observation
the System Manager  Test   
 Other __________
Review sample results for  Direct Observation
flags, codes or messages  Test   
 Other __________
Cleaning/Replacing System HELP
Procedures as needed
Clean (Bleach) the Apertures  Direct Observation
 Test   
 Other __________
Clean the Aspiration Probe  Direct Observation
 Test   
 Other __________
Clean BSV externally  Direct Observation
 Test   
 Other __________
Clean the Pneumatic  Direct Observation
Supply Fan Filter  Test   
 Other __________
Clean the STM  Direct Observation
 Test   
 Other __________
Clean the AMTC  Direct Observation
 Test   
 Other __________
Clean the Vacuum Trap  Direct Observation
  
 Test
Rev. A (June 2014) 41 of 118
UUNICEL
Y N Initial Initial
 Other __________
Clean the Optical Sensors  Direct Observation
 Test   
 Other __________
Clean the Cassettes
 Test   
 Other __________
Replace the Aspiration Probe  Direct Observation
 Test   
 Other __________
Replace Reagent Containers  Direct Observation
 Test   
 Other __________
Replace Waste Containers  Direct Observation
 Test   
 Other __________
Replace the Pneumatic  Direct Observation
Supply Module (PSM)  Test   
 Other __________
Replace the Hand-held  Direct Observation
Bar-Code Scanner  Test   
 Other __________
RESOURCES IFU
System HELP
 Test   
Training Material
 Other __________
Laboratory Protocol
Rev. A (June 2014) 42 of 118

Cellular inter: Pattern consistent with NRBC detection during a CBC only cycle. This
Cellular Interference Suspect message is not associated with a Review (R) flag.
Cellular inter: Poor separation between a WBC population and interference. WBC
correction was performed as a best estimation, and the WBC was flagged with R.
Data Fusion saw NRBC and corrected the WBC count. The “R” flag means review the
results. CBC only was ordered or run.
A CBC was only ordered. No NRBC % results or NRBC Suspect Message obtained.
Rev. A (June 2014) 43 of 118

Cellular Inter: Pattern consistent with NRBC detection during a CBC only cycle. This
Cellular Inter: Poor separation between a WBC population and interference. WBC
results.
A CBC was only ordered. No NRBC % results or NRBC Suspect Message obtained.
Why do I have both Cellular inter Messages? (Suspect and System)
Pattern consistent with NRBC detection during a CBC only cycle.
Poor separation between a WBC population and interference.
Yellow results means: Outside of Action Limits Low or High.
Red results means: Outside of critical limits.
Rev. A (June 2014) 44 of 118

UniCel DxH 800/ DxH 600
Data Fusion saw NRBC cells but no correction of the WBC count. Why????
WBC was not corrected because the NRBC cells were less than 35 fL in size.
Rev. A (June 2014) 45 of 118

Data Fusion saw NRBC cells and corrected the WBC count. Why????
WBC was corrected because the NRBC cells were greater than 35 fL in size.
Rev. A (June 2014) 46 of 118

Variant Ly
Cellular Inter: Poor separation between a WBC population and interference. WBC
results.
CDR will report NRBC % if enable. That is why no Cellular inter (Suspect Message)
obtained.
results.
Rev. A (June 2014) 47 of 118

WBC was corrected but no Cellular Interference flag was posted. Why????
No suspect message “Cellular inter” because CDR was ordered.

No system message “Cellular inter” because it was sure of the correction.
The DxH 800 technologies: Data Fusion, Watershed, Templating Matching, Shape
Signature. An exact match may give you a message, but may not flag the result with an
“R “because it is confident of the correction.
Rev. A (June 2014) 48 of 118

Additional Data

1 3
1: Exception Status
2: Diff Count / Time
3. Flagging Sensitivity
Rev. A (June 2014) 49 of 118

Additional Data

1 3
1: Exception Status
2: Diff Count / Time
3. Flagging Sensitivity
Rev. A (June 2014) 50 of 118

Troubleshooting Procedures
Cleaning the Specimen Transport Module (STM)

Step Action
1 Power OFF the SPM. (Specimen Processing Module)
2 Remove any cassettes in the input or output buffer.
3 Remove the Transport Shield.
Grasp the front of the STM on both sides; then lift and pull towards you as if opening a
4 drawer.
5 Examine the drip trays behind the mixer and behind the pending buffer.
Use a lint-free tissue dampened with DI water to clean the inside of the STM and drip
6
trays, wiping up any spills or buildup.
7 Dry the inside of the STM by wiping with a dry lint-free tissue.
Rev. A (June 2014) 51 of 118

Cleaning the Optical Sensors

Step Action
2 Remove the Transport Shield and lift the Front Cover.
Locate the optical sensor. Three are located at the input buffer and two at the output
3
buffer. Remember to clean the sensor located at the end of the output buffer.
4 Dampen a very fine lint-free swab with DI water.
5 Swab the optical sensor to remove buildup.
6 Lower the Front Cover and Replace the Transport Shield.
7 Power ON the SPM.
Rev. A (June 2014) 52 of 118

Cleaning the BSV (Blood Sampling Valve) Externally

Step Action
3 Lift the Front Cover.
4 Locate the BSV.
5 Dampen a piece of lint-free tissue with DI water and wipe down the outside of the BSV.
6 Wipe out the drip tray under the BSV with a lint-free tissue dampened with DI water.
7 Lower the Front Cover.
8 Replace the Transport Shield.
9 Power ON the SPM
Rev. A (June 2014) 53 of 118

Cleaning the Pneumatic Supply Module (PSM) Fan Filter

Step Action
2 Unscrew the power / communications cable from the Pneumatic Supply Module (PSM)
3 Locate the filter on the top of the PSM.
Using a flathead screwdriver or your hand, loosen the thumbscrews that secure the
4 filter retaining plate and remove the filter from the PSM.
5 Wash the filter with soap and water, rinse and let it dry completely.
Return the clean air filter and retaining plate to their original location and fasten the
6
thumbscrews to secure the filter.
Connect the power / communications cable connector to the power / communication

port on the PSM.
a. Line up the holes of the connector with the pins in the port.
7
b. Push the connector into the port so that the pins in the port are inserted
into the connector.
c. Fasten the connector by turning the connector clockwise.
8 Power ON the SPM.
Rev. A (June 2014) 54 of 118

Cleaning the Air Mix Tempetarure Control (AMTC) Module

Step Action
3 Dampen a lint-free applicator stick with DI water.
Note You can use a fabric-tipped applicator to clean the top of the AMTC.
Carefully use the tip of the applicator stick to wipe debris away from the port on the
AMTC.
Note It is important to avoid getting debris in the holes at the top of the AMTC during this
procedure.
5 Replace the Transport Shield.
6 Power ON the SPM.
Rev. A (June 2014) 55 of 118

Cleaning Aperture (Bleaching)

Step Action
Prepare a 20 mL solution of bleach and DI water. Mix together:
 10 mL of high quality, fragrance-free, gel-free bleach (5 to 6% solution of

sodium hypochlorite – available chlorine) and
 10 mL of DI water.
1
Put 20 mL of DI water in a second container.
3 From the System Manager, select Menu > Diagnostics > Dx Tools.
Select the Maintenance tab to display the Diagnostic Procedures - Maintenance

4
screen.
5 Select Clean Apertures from the drop-down list.
6 Select Start. The system places the SAM in safe mode and drains the baths.
7 Remove the Transport Shield and lift the Front Cover.
8 Locate the bleach probe.
Rev. A (June 2014) 56 of 118

When prompted, place the bleach probe into the container of bleach-DI water solution.
9 The system aspirates the bleach solution, draws it through the apertures, and then
drains the baths.
Note that a dialog box displays the Time Elapsed as the aperture bleaching takes
place.
10
Note: A maximum of 15 minutes of aperture bleaching is suggested.
Select: OK to end the bleaching and continue with the procedure.
When prompted, place the bleach probe in DI water (a container of approximately 20

11
mL of DI water). The system aspirates the DI water.
12 Follow the directions on the screen.
Lower the Front Cover and replace the Transport Shield.

13
The system displays System performing Clean CBC Apertures procedure. Please wait.
14 Select Finish.
Run Shutdown.
Note: Do not select the Perform Startup (Daily Checks) after Shutdown check box on
15
the Manual Shutdown dialog box.
Refer to the Remove Cleaner section in CHAPTER 8, Shutdown for instructions.
Select Cancel from the local navigation bar when you see the timing bar at the lower
16
right-hand side of the screen start to count down.
17 Run Daily Checks. Refer to Run Daily Checks.
Rev. A (June 2014) 57 of 118

Cleaning the Vacuum Trap

Step Action
1 Power OFF the SPM (Specimen Processing Module)
2 Locate the vacuum trap.
Holding the top of the vacuum trap with one hand, use the other hand to unscrew the
3
vacuum trap jar.
Empty the vacuum trap jar according to your local environmental regulations and your
4 laboratory’s procedures.
5 Rinse the vacuum trap jar with water, and then shake out the excess water.
6 Use a lint-free tissue to dry the vacuum trap jar.
Dampen a lint-free tissue with DI water and wipe down the black center post inside the
7
vacuum trap assembly. Spin the center post to wipe down the entire surface.
Replace the vacuum trap jar.

a. Ensure that the black center post is not stuck in the up position.
8
b. Carefully align the threads on the vacuum trap jar with the threads on the
vacuum trap assembly and screw the vacuum trap jar into place.
9 Wipe up any spills.
10 Power ON the SPM.
Rev. A (June 2014) 58 of 118

Cleaning the Aspiration Probe

Step Action
Beckman Coulter recommends that you do the Dispense Diluent procedure after
Note
cleaning the aspiration probe to verify the integrity of the aspiration path.
1 Select Menu > Diagnostics > Dx Tools > Maintenance tab.
2 Select Clean Aspiration Probe from the drop-down list.
Select Start. The system:

 Moves the SAM to a position between the cassette mixer and the AMTC
module.
3
 Lowers the probe assembly and extends the aspiration probe, exposing it
behind the transport shield.
 Flushes the outside of the aspiration probe with diluent as the probe retracts.
If necessary, press (F10) Access System Monitor to view the Probe Waste Vacuum
4 sensor’s state on the System Monitor screen.
Perform the Dispense Diluent procedure. Refer to the Dispense Diluent Procedure
5
(Menu > Diagnostics > Dx Tools > Maintenance tab)
6 When the procedure is complete, select Finish.
Rev. A (June 2014) 59 of 118

ZAP Apertures
Step Action
This procedure applies voltage across RBC and WBC apertures to remove protein
buildup.
The system:
Note
 Drains and rinses the baths.
 Applies the ZAP voltage across the apertures multiple times.
 Monitors and displays the ZAP voltages on the System Monitor screen.
2 Select Zap Apertures from the drop-down list.
3 Select Start to initiate the procedure.
Press (F10) Access System Monitor and select Volt/Temp to view the Zap Aperture
4 voltages sensor status on the System Monitor.
5 Select Finish.
Rev. A (June 2014) 60 of 118

Flush Flow Cell

Step Action
The Flush Flow Cell procedure is used to clear blockage or flush debris from the flow
cell. The flow cell comprises an upper and lower chamber connected by an aperture.
Four procedures are available:
 The Flush Flow Cell procedure flushes both chambers and flushes the aperture
to clear blockage.
 The Flush Lower Flow Cell procedure flushes the lower chamber only.
Note  The Flush Upper Flow Cell procedure flushes the upper chamber only.
 The Flush Flow Cell with Cleaner procedure flushes both chambers with
cleaner. Then, a timer lets you leave the cleaner in the flow cell from 0 - 30
minutes. After the allowed time has elapsed, the system removes the cleaner
from the flow cell and cleans all the areas contacted by the cleaner.
If the remove cleaner operation is not successful, the system will not allow any other
operation except remove cleaner.
2 Select Flush Flow Cell from the drop-down list.
Select a procedure and select OK.

4 Note: If you select With Cleaner, a Time in Cleaner text box will be displayed on the
Flush Flowcell dialog box. The default time is 5 minutes. Enter your desired time in the
cleaner in the text box and select OK.
5 Select Finish.
6 Perform Daily Checks.
Rev. A (June 2014) 61 of 118

Cycle DV (Distribution Valve)

Step Action
The Cycle DV procedure verifies the DV motor, clutch, and optical sensors. The
system:
 Rotates the DV to the right and then to the left 10 times, without moving any
Note liquids.
 Monitors the DV home and DV segment sensors.
 Displays the sensors’ state on the System Monitor screen.
2 Select Cycle DV from the drop-down list.
Press (F10) Access System Monitor and select SAM to view the sensors’s status on
4 the System Monitor SAM/ Blood Transport panel.
5 Select Finish.
Rev. A (June 2014) 62 of 118

Troubleshooting Procedure
Replace the PSM (Pneumatic Supply Module)
Step Action
1 Using the handles on the PSM, lift it out of the box.
2 Turn the PSM over so the bottom of the PSM is facing up.
On the bottom of the PSM, use a Phillips screw driver to remove the four Phillips-head
screws located inside the four socket-head screws, then use the enclosed nut driver to
remove the four socket head screws.
The PSM ships with feet that are intended for floor cabinet use only. If the DxH is
installed on a bench top or non BCI cabinet, the PSM will be placed on the floor or a
shelf; you must remove the feet and replace them with the optional feet which are
required to support the full weight of the PSM.
When returning a defective pneumatic supply includes the unused feet and provided
5 tool. Please replace the four socket head screws and Phillips-head screws on the
pneumatic supply.
Rev. A (June 2014) 63 of 118

Rev. A (June 2014) 64 of 118
UniCel DxH Slidemaker Stainer
Coulter Cellular Analysis
System
Rev. A (June 2014) 65 of 118

System Overview
3
2
1. DxH Slidemaker Stainer
2. System Manager
3. Handheld Bar Code Scanner
Rev. A (June 2014) 66 of 118

System Overview
2
1 3
5
10
9 6
8 7
1. Front Cover 6. Cassette Input Buffer
2. Beacon 7. Power Switch
3.Status Indicators 8. Single-Tube Station
4. Ejector Door 9. Cassette Output Buffer
5. Slide Chute 10. Input/Output (I/O) Drawer
Rev. A (June 2014) 67 of 118

System Overview
1
Left side of the Slidemaker Stainer:
1. Front Cover
2
2. Input/Output (I/O) Drawer
Open/Close Button 3
3. I/O Drawer 4
4. Transport Shield
Right side of the Slidemaker Stainer:
1. Front Cover
1 7
2. Slide Stack
2
3.Transport Shield 6
4. Floor Stand 3
5
5. Slide Printer Cover
6. Cartridge Cover
4
7. Upper Right-Side Cover
Rev. A (June 2014) 68 of 118

System Overview Optional Floor Stand
1
9
2
3
4 10
5 6
7 11
8
12
1. Deionized Water 7. Cleaner Reagent
2. Supply 4 (S4) 8. Stain Waste
3. Supply 3 (S3) 9. Diluent 1 Container
4. Supply 2 (S2) 10. Diluent 2 Container
5. Supply 1 (S1) 11. Biohazard Waste 1 Container
6. Extra Cleaning 12. Biohazard Waste 2 Container
Rev. A (June 2014) 69 of 118

Daily Checks
Step Action
Select the System Status icon . Look at the SMS screen to give you an overview
1
of basic information about the instrument.
Active = online and ready Alert Status Icon color meaning:

Inactive = offline and not Red = error
ready
Disabled = maker or stainer Yellow = warning
not available for use Neutral = OK
Enabled = maker and/or
stainer available for use Displays if unit is
Online, Offline, in
Diagnostics, or Stopped
Printer Status:
LIS Status: Status displays Auto Print
Green = LIS Interface ON Disabled, if Disable All Auto
Gray = LIS Interface OFF Printed Reports is selected from
Print >Print Printer Setup
Click on the Details Status button or double click on the instrument picture
2
on the screen.
Rev. A (June 2014) 70 of 118

Step Action
DATE & TIME of last MAKER & STAINER

DAILY CHECK and STATUS
SHUTDOWN Cycles
DEFAULT SLIDE ORDER

CASSETTE PREPARATION
SINGLE-TUBE PREPARATION
TRANSPORT STATUS
If the date and time are a neutral color, all is OK. If they are red, there is a problem.
Look at the Maker and Stainer status for Active, Inactive, or Disabled state.
3
This is where you view the status of the maker and stainer.
Transport Status may be Online, if processing samples. Offline, for special cycles and
procedures. Diagnostics or Standby, for certain diagnostic tests. Stopped, when
4 system is commanded by the operator to stop the process, or Disconnected, when the
system is powered off.
Select the Daily Checks alert status icon

5 or
Menu>QA>Daily Checks.
6 Select Daily Checks from the local navigation bar.
Rev. A (June 2014) 71 of 118

Step Action
When the system displays: You have requested to perform Daily Checks.
Select OK to run Daily Checks

or
Select Cancel to go back to the previous screen.
8 This cycle will take approx. 3 minutes.
Viewing Daily Checks Information

Step Action
Look at the Daily Checks alert status icon . If it is neutral, all is well. If it is red, then
1 something failed and needs review. You can view the daily checks result information for
the Summary, SMS, and Supplies.
The Daily Checks must pass or be reviewed in order to run specimens. If Daily Checks
passes, the Review button is disabled.
Note
Rev. A (June 2014) 72 of 118

Step Action
Select the Review button from the local navigation bar to clear any system failures and
2
to return to neutral.
3 Select the View Log button to review the Daily Checks tab of the History Log.
When you click “Review” for failed results, you are acknowledging the failure and the
Note need to repeat the Daily Checks. Be sure to document per your regulatory
requirements. You could add a comment in the History Log.
4 After resolving the cause, repeat Daily Checks.
A dialogue window appears asking: Are you sure you want to acknowledge the
6
Daily Checks failure? Press OK to continue.
Enable Automatic Daily Checks

Step Action
From the Daily Checks screen, select Auto Configuration>Configure Daily Checks to
display the Auto Daily Checks Configuration dialog box.
2
1
Rev. A (June 2014) 73 of 118

Step Action
Select Enable Automatic Daily Checks to enable running daily checks automatically
on your system. Follow the steps to enable/configure automatic Daily Checks.
You may, select the radio button Perform daily checks after shutdown from the set-
3
up screen.
If you selected Perform daily checks after shutdown, select OK to save your
selection
4 or
If you selected Perform daily checks at a specified time, select a time and a day, and
select OK to save your selection.
Viewing Past Results/Adding Comments

Step Action
1 Select the View Log button from the Local Navigation Bar.
2 You may select one date at a time to review.
Rev. A (June 2014) 74 of 118

Step Action
Once highlighted in blue, select the Details button below to view all tabs of those
results. Select View Log to return to the log or the large back arrow on the upper left to
go back one screen.
With any date highlighted, select the Comments button below to add a comment.
Rev. A (June 2014) 75 of 118

Step Action
5 Select OK to save comment.
Once saved, you will see a yellow, page icon appear to let you know there is a comment
there. You may go back to a comment to edit it as well.
Printing/Auto-Printing
Step Action
You may manually print individual results by selecting the print icon from the utility area
1
at the upper right .
On the daily checks screen, you may print the Daily Checks Auto Report.
If you are on Daily Checks: View Log>Log Type>Data Summary Logs, you may print:
Entire log, All filtered, or Selected entries by clicking the print icon from the utility
area at the upper right.
Rev. A (June 2014) 76 of 118

Step Action
3 You may decide to have the Daily Checks print automatically.
4 To do this, return to the Daily Checks screen by selecting the Daily Checks icon.
Select the Auto Report button from the Local Navigation bar. This opens the Daily
Checks Auto Report window.
5
You may select to print automatically by checking the Enable auto report generation
box.
Rev. A (June 2014) 77 of 118

Samples Processing
Cassette Presentation (LIS Bidirectional Download to Pending)
Step Action
Place the instrument online. The instrument will automatically begin cycling the
1
cassettes.
2 Ensure your specimens have been collected and stored properly.
Load the specimens into the cassette.
Place the cassettes into the input buffer and slide the cassette forward until the
green light is on.
Rev. A (June 2014) 78 of 118

Step Action
1 Select the Single-tube Presentation icon.
The single-tube Presentation Dialog Box will come up.
Place the specimen in the grooved area in front of the barcode reader
or
Enter the Sample ID manually
(You may use alpha or numeric characters)
3 or
Use the bar-code scanner to scan the label. Your handheld bar-code scanner is a
camera that takes a picture of the bar-code.
Note: Move the label closer if the bar-code scanner does not read the information.
Once the bar-code is read, you will hear a beep.
4 Press Enter.
Place the tube in the correct cradle position. The left (lavender) position adapts to
both open and closed vial tubes from 12 to 13 mm in diameter. The right (green)
5 position holds microtainer tubes. The cradle retracts and the instrument processes
the specimen. When the instrument is ready to process the next specimen, the
system sends the cradle back out again.
Select the Exit button on the Single-Tube Station dialog box to exit from the
6
Single-Tube Station when sample processing is complete.
Rev. A (June 2014) 79 of 118

Adding a Test Order (Menu>Worklist)
Step Action
Select Single-tube Presentation Icon

1 or
Select Menu>Worklist.
Select the Slides tab, Pending for the Filter Name, and Add Order button from
the local navigation bar
2 or
Select the Slides tab and select the Add Order button from the local navigation
bar.
On the Add Order screen, enter the specimen ID manually or by using the bar-
code scanner.
4 Select the desired radio buttons for Make, or Make and Stain.
Select the number of slides to be made, and/or made and staining using +/-
5
buttons or editing the default value of 1.
If desired, select the Patient button and Add Patient from the local navigation bar
6
to add patient demographics.
7 Enter patient information and select OK.
8 Select the Submit button from the local navigation bar.
Rev. A (June 2014) 80 of 118

View Basket Details
Step Action
1 Select to display the System Status screen.
2 Select the Details Status from the local navigation bar.
Select Basket Management from the local navigation bar to view the Basket
Management screen. On this screen, you can manage baskets or get basket
information anywhere in the Stainer module and Basket Transport Module.
Select Basket Details from the local navigation bar to display the Basket Details
dialog box containing specific patient information for the slides in the selected
location.
View the details and select OK when you are done

5 or
Select Cancel to exit the screen.
Rev. A (June 2014) 81 of 118

Advance Baskets
Follow these steps to advance a selected basket to the I/O drawer.
Step Action
Select the Details Status from the local navigation bar on the DxH Slidemaker
2
Stainer Status screen or double click on the instrument icon.
Management screen.
4 Select the basket to advance.
Select Advance Basket from the local navigation bar to display the Basket Details
dialog box.
5 Note:
The baskets/slides may be wet if you select Advance Basket before the pre-stain
drying cycle is complete.
On the DxH Solutions dialog box, select OK to forward the basket to the I/O
drawer.
6
Rev. A (June 2014) 82 of 118

Select Basket to Stain Next
Follow these steps to schedule the next basket with slides for staining.
Step Action
Management screen.
4 Select the basket to stain next.
Select Stain Next from the local navigation bar to display the Stain Next dialog
box.
Select OK to save you changes

6 or
Rev. A (June 2014) 83 of 118

Cancel Staining
This command cancels all staining in progress and all slides waiting to be stained.
Slides that are in the stainer will advance to the dryer and then to the I/O drawer.
Slides in the basket elevator will advance to the I/O drawer.
Step Action
Management screen.
Select Cancel Staining from the local navigation bar to display the Cancel
Staining dialog box.
Select OK to save your changes

5 or
Rev. A (June 2014) 84 of 118

Loading or Removing Baskets at the Input/Output (I/O) Drawer
Risk of personal injury and contamination. The processing areas may contain prepared smears
and/or broken glass with biohazardous material. Handle with care and avoid skin contact.
Risk of personal injury and contamination. The instrument could continue to move or could have
delayed movement. Wait a few seconds to ensure that all movement has ended before pulling
the I/O drawer.
Step Action
Press the round lighted button to access the input/output (I/O) drawer.
Once the drawer has stopped moving (it may take a few seconds), pull the handle
towards you until it clicks.
Remove and/or load the baskets.

The indicator lights are:
3  Blinking green – when the basket is ready for you to remove it
 Steady green – when the basket is ready to be used by the system
 Blinking red – when the basket is in an error state
 Blinking blue – when a basket is loaded with slides for staining only
Push the handle toward the instrument until it stops. The drawer will automatically
return to its position inside the instrument.
Rev. A (June 2014) 85 of 118

Staining Samples Manually (Stain-Only Priority)
Risk of personal injury and contamination. The processing areas could contain prepared smears
and/or broken glass with biohazardous material. Handle with care and avoid skin contact.
Some samples may require manual staining, such as small blood draws (pediatric)
taken with microcollection devices.
Follow these steps to manually stain a sample.
Step Action
Fill in an empty basket with slides ready to stain (painted end on top so as not to
stain the sample information).
Place the basket back in the I/O drawer in any open/empty position from 1 to 6.
Press the number where the basket is placed.
Push the drawer in for staining to begin.
Rev. A (June 2014) 86 of 118

Making Slides (No Staining)
Risk of injury and biohazardous contamination. Clean up any broken glass or blood spill as
quickly as possible. Handle with care. Avoid skin puncture. Dispose of all contaminated
disposable cleaning materials and broken slides in accordance with your local regulations and
acceptable laboratory practices.
Risk of contamination. The prepared smears contain biohazardous materials. Handle with care
and avoid skin contact.
Follow these steps to make slides that do not require staining:
Step Action
Select Make Slide on the Default Test Order for the applicable cassette or single-
tube presentation
or
1
Add or edit a test from the Worklist Pending tab
or
Load samples for the processing of downloaded Test Orders from the LIS.
Rev. A (June 2014) 87 of 118

Shutdown
The DxH Slidemaker Stainer allows you to program the length of time the instrument is
in Shutdown. The default time is 30 minutes. This is the minimum time required for
Shutdown.
At the end of your programmed time, the system removes DxH Cleaner and replaces it
with DxH Diluent. It then awaits a Daily Checks cycle, performed manually or
automatically.
If a Shutdown cycle needs to be interrupted, just cancel and then request a Daily
Checks cycle.
Step Action
From the Daily Checks screen, select the Shutdown button to display the Manual
1
shutdown dialog box.
Select the Perform Daily Checks After Shutdown check box to automatically begin
3
Daily Checks after shutdown is completed.
Type a number in the Time in Cleaner Hours and Minutes check boxes to determine
the time in cleaner.
4 Note: No other cycles or System Manager actions can be performed until the
Shutdown cycle has completed. Once the Shutdown cycle is complete, a countdown
timer bar appears at the bottom of the Daily checks screen to let you know how much
time remains for the cleaning.
5 Select OK to begin shutdown or Cancel to exit.
Rev. A (June 2014) 88 of 118

Enable Automatic Shutdown
Step Action
From the Daily Checks screen, select Auto Configuration>Configure Shutdown to

1
display the Auto Shutdown Configuration dialog box.
Select Enable Automatic Shutdown to enable running Shutdown automatically on

2
your system.
In your Laboratory you may set-up shutdown as follows:

 Type the time for automatic shutdown in Hour and Minutes in the text box.
3  Select Daily or select specific days of the week to run Shutdown automatically.
 Type the time the cleaner should remain in the DxH Slidemaker Stainer during
the shutdown procedure in Hours and Minutes.
4 Select OK to save your selections.
Rev. A (June 2014) 89 of 118

Supplies
For any non-Beckman Coulter reagent, select Setup Other and manually enter the bar code
information. You can enter up to five supplies.
Step Action
Select to display the Supplies screen.

1 or
Select Menu>Supplies>Slidemaker tab.
On the Supplies screen, select the Setup button from the local navigation bar.
Rev. A (June 2014) 90 of 118

Step Action
On the Setup Supplies dialog box, scan or manually enter the bar codes into Bar Code
fields 1 and 2, and select OK.
3
Note: The fields on the Supplies screen are automatically populated from the bar code
information. The percent (%) remaining of each supply appears on the bottle graphics.
From the Supply drop-down list, choose Diluent 1 or Diluent 2 to be replaced and
select OK.
4
After entering the information, the system fills in the reagent type, lot number, and
other information on the left side under Supply Information Preview.
Select OK to verify the reagent has been replaced

5 or
6 Repeat steps 1 to 5 above for each remaining supply.
Configure Low-Level Notification

Step Action
1 Select or Menu>Supplies
From either the Slidemaker or Stainer tab, select the Config Low Level button from
the local navigation bar on the Supplies screen.
2
Note: You must configure the low-level notification for reagents for both the Slidemaker
and Stainer tabs.
On the Configure Low Level Condition screen, select a low-level percentage from the
3
drop-down box (default is 10%).
Select OK to save your changes

4 or
You may set the low-level warning from 5% to 15%. When this occurs, the Supplies
Note
Alert Status Icon is yellow. If a supply is out, the icon is red.
Rev. A (June 2014) 91 of 118

Setting Up Beckman Coulter Stainer Reagents
For any non-Beckman Coulter reagent, select Setup Other and manually enter the bar code
information. You can enter up to five supplies.
Follow these steps to set up reagents for the stainer.

Step Action
Select to display the Supplies screen.

1 or
Select Menu>Supplies>Stainer tab.
On the Supplies screen, select the Setup button from the local navigation bar.
Rev. A (June 2014) 92 of 118

Step Action
Scan or manually enter the barcodes, into Bar Code fields 1 and 2, and select OK.
4 On the Supply field, choose the reagent to be replaced from the drop-down list.
On the displayed message, select OK to verify that the reagent has been replaced
5 or
6 You would repeat steps 3 to 5 above for each remaining supply.
Select Details from the local navigation bar to display information for each supply.
Note: Slidestainer Supplies Details indicates the supplies required for the active
staining protocol set for the instrument.
Supplies History Logs

There are two history logs associated with Supplies. One is located under Event Logs
and the other is located under Data Summary Logs.
Supplies Event Log: This log records any changes or alerts associated with the
supplies.
Supplies Data Summary Log: This log records all the information on each new
container set up on the System.
Rev. A (June 2014) 93 of 118

Loading Slides
The software supply icon turns yellow when the slide level is low.
The software supply icon will turn red when the slides are completely out. The system
will go offline until more slides are loaded. Go back online to continue processing the
slides.
Risk of injury and biohazardous contamination. Handle slides with care to avoid skin puncture.
Clean up any broken glass or blood spill as quickly as possible. Dispose of all contaminated
disposable cleaning materials and broken slides in accordance with your local regulations and
acceptable laboratory practices.
NOTE: There are 2 sensors on the slide chute. One of the sensors, checks the slide inventory below ½
(approx 75 slides). When this happens the Supplies Icon will be in Yellow. The other sensor will stop the
Slidemaker Stainer. This will occur when there are 12 slides left in the chute. The Supplies Icon will be in
red.
Step Action
Open the slide ejector door.
Remove slides from the box handling them by the painted end.
Rev. A (June 2014) 94 of 118

Step Action
Load the slides into the slide chute with the painted side up and to the right.
Note: You can load up to 172 slides. Be aware that slides exposed to high humidity
may stick together.
Close the slide ejector door.
Rev. A (June 2014) 95 of 118

Disabling/Enabling
Disable the Stainer
Step Action
2 Select Details Status.
3 Select Module>Disable Stainer from the local navigation bar.
On the Disable Module dialog box, select the checkbox and select OK to disable
4
the stainer.
Select YES when the system displays:

5 You have requested to temporarily disable a module. Do you want to continue with
the request?
Enable the Stainer
Step Action
3 Select Module>Disable Stainer from the local navigation bar.
On the Disable Module dialog box, deselect the check box and select OK to enable
4
the stainer.

You have selected to enable a module. The System will automatically perform a
5
Prime. Performance verification must be performed before patient samples can me
analyzed. Do you want to Continue?
Rev. A (June 2014) 96 of 118

Disabling/Enabling
Disable the Maker
Step Action
3 Select Module>Disable Maker from the local navigation bar.
On the Disable Module dialog box, select the check box and select OK to disable
4
the maker.

5 You have requested to temporarily disable a module. Do you want to continue with
the request?
Enable the Maker
Step Action
3 Select Module>Disable Maker from the local navigation bar.
On the Disable Module dialog box, deselect the check box and select OK to enable
4
the maker.

You have selected to enable a module. The System will automatically perform a
5
Prime. Performance verification must be performed before patient samples can me
analyzed. Do you want to Continue?
Rev. A (June 2014) 97 of 118

Enabling/Disabling Batching
Step Action
The system must be offline and no staining should be in progress in order to

perform this procedure.
Note
You can use batching when you want to analyze all your specimens with the same
default test panel (for example, when the LIS is down).
3 Select the Batching from the local navigation bar.
Select the checkbox to enable Batching or deselect the checkbox to disable

Batching.
Note All results are labeled as Default Slide Order.
5 Select OK.
Rev. A (June 2014) 98 of 118

Configure Auto Stop Settings
Step Action
1 Select System Status>More.
2 Select Auto Stop from the local navigation bar.
On the Configure Auto Stop Settings dialog box, select from the following options:
Select OK to confirm your selection

4 or
Rev. A (June 2014) 99 of 118

Cleaning Procedure
1. Drain ALL Baths.
2. Transfer pickup tubes of supplies 2 and 4 to the Methanol

cleaning bottles. Wrap pickup tubes assembly in clean gauze to
prevent dripping.
3. Individually fill baths (2 and 3) with Methanol.
4. Allow the Methanol to sit in the baths for at least 15 minutes.
5. Drain baths (2 and 3) individually or you may select Drain All

Baths.
6. Remove pick tubes assembly from the Methanol cleaning

bottles and turn them upside-down. Wrap pickup tubes
assembly in clean gauze to prevent dripping.
7. While holding pickup tubes individually fill baths (2 and 3) with

air. Allow air to flow thru pickup tubes for less then 2 minutes
and press Cancel. (This will prevent the low supply warning).
8. Transfer pickup tubes assembly back into supplies (2 and 4).
9. Select the Finish icon from the Local Navigation Bar.
10. Clean or Replace baths, especially baths (2 and 3).
11. Clean the probes by wiping them with Methanol.
12. Place the DxH Slidemaker Stainer Online to Continue Working.
Rev. A (June 2014) 100 of 118

System Components (Front Side)
Blood Dispense
Detector Probe
Hemasphere
Aspiration
Probe
Slide
Chute
Printer
Bar Code
Ribbon
Reader
Printer
Head
Stain
Drawer
I/O
Drawer Smear
Shuttle
Output
Buffer
Mix Stations
Input
Single Tube Bar Code Buffer
Presentation Reader
Rev. A (June 2014) 101 of 118

System Components (Right Side)
Printer
Basket Ribbon
Elevator
Rear
Basket Print
Elevator Head
Front
Print
Shuttle
Ribbon
Drive Motor
Smear
Shuttle
Motor
Slide Printer
Printer
Print Ribbon
Head
Ribbon
Print Drive Motor
Shuttle
Rev. A (June 2014) 102 of 118

System Components (Left Side)
Input/Output
(I/O) Drawer
Button
(I/O)
Drawer
Dispense
Probe
Aspiration
Probe
Overflow
Chamber
Output
Buffer
Rev. A (June 2014) 103 of 118

System Components (Slide Stainer)
Hemasphere
Blood
Detector
Lee Valve
Aspiration
Probe
Dispense
Probe
Fill Probe
Bar Code
Reader Over Flow
Sensor
Drain
Probe
(Tube)
Stain
Drawer
Bath
Bath Tray
Access
Handle
Rev. A (June 2014) 104 of 118

Basket Management
To view the Basket Management information, the operator selects Basket Management from the local navigation bar. On this screen,
you can manage baskets or get basket information anywhere in the Stainer module and I/O drawer.
An example of this is the Basket that has been selected by the operator on this screen capture. When a basket is selected, it will have a
lime green halo around it. The operator may refer to the Basket Data box, where the following information may be found.
Type = Make and Stain or Stain Only.
State = refers to what is happening to the slide at this moment.
Priority = Routine, STAT, ASAP.
Duration Time = based on the stain protocol settings, refers to how long the slide is programmed to be in that specific location. (ie:
staining bath, elevator, dryer). In this example, its is Bath 2.
Elapsed Time = refers to the amount of time the slide has already been in that specific location.
Remaining Time = refers to the amount of time remaining in that location. (ie: staining bath, elevator, dryer)
ETC = refers to Estimated Time to Complete
Rev. A (June 2014) 105 of 118
Loading/Removing Baskets
Input/Output (I/O) Drawer
The indicator lights in the I/O drawer mean the following:
• Blinking Green – when the basket is ready for you to remove it

• Steady Green – when the basket is ready to be used by the system
• Blinking Red – when the basket is in an error state
• Blinking Blue – when a basket is loaded with slides for staining only
Rev. A (June 2014) 106 of 118

Rev. A (June 2014) 107 of 118
Rev. A (June 2014) 108 of 118
UNICEL® DXH SLIDEMAKER STAINER COULTER® CELLULAR ANALYSIS SYSTEMS
Achieved? N/ Trainee Trainer
Task Resource How Measured? Date
Y N A Initial Initial
IDENTIFY POTENTIAL HAZARDS AND
SAFETY FEATURES
Review MSDS www.beckmancoulter.co  Direct Observation
m  Test   
MSDS manual in lab  Other __________
Recognize Hazard Symbols System HELP  Direct Observation
 Test   
 Other __________
IDENTIFY AND BRIEFLY EXPLAIN System HELP  Direct Observation
FUNCTION OF ANALYZER COMPONENTS  Test   
 Other __________
OVERVIEW OF SCREENS AND System HELP
SOFTWARE Training Material
Identify System Manager Icons and  Direct Observation
Screens  Test   
 Other __________
Use System HELP  Direct Observation
 Test   
 Other __________
MANAGE REAGENTS AND System HELP
CONSUMABLES Training Material
Setup new lot number for Reagents  Direct Observation
and Stains  Test   
 Other __________
Replace reagent container  Direct Observation
 Test   
 Other __________
Loading Slides  Direct Observation
 Test   
 Other __________
DAILY CHECKS AND SHUTDOWN System HELP
PROCEDURES Training Material
Perform a Daily Checks Cycle & Review  Direct Observation
the Results  Test   
 Other __________
Perform a Shutdown  Direct Observation
  
 Test
Rev. A (June 2014) 109 of 118
 Other __________
SETTING UP THE SLIDEMAKER System HELP  Direct Observation
Training Material  Test   
 Other __________
Setting up Default Slide Order/Set Number  Direct Observation
of Slides  Test   
 Other __________
Setting up Basket Settings  Direct Observation
 Test   
 Other __________
Set Pre-Stain Drying Type  Direct Observation
 Test   
 Other __________
Configure the Slide Label  Direct Observation
 Test   
 Other __________
Set up Smear Settings  Direct Observation
 Test   
 Other __________
SETTING UP THE STAINER System HELP
Training Material
Laboratory Protocol
Set Active Staining Protocol  Direct Observation
 Test   
 Other __________
Add/Edit a Staining Protocol  Direct Observation
 Test   
 Other __________
Configure Baths (mapping) to the Bath  Direct Observation
Location  Test   
 Other __________
Copy Staining Protocol  Direct Observation
 Test   
 Other __________
Delete Staining Protocol  Direct Observation
 Test   
 Other __________
Rev. A (June 2014) 110 of 118

Setting up a Quick Rinse  Direct Observation
 Test   
 Other __________
DISABLING/ENABLING THE SLIDEMAKER System HELP
STAINER Training Material
Disable the Slidemaker  Direct Observation
 Test   
 Other __________
Enable the Slidemaker  Direct Observation
 Test   
 Other __________
Disable the Stainer  Direct Observation
 Test   
 Other __________
Replace the Hand-held  Direct Observation
Bar-Code Scanner  Test   
 Other __________
SAMPLE PROCESSING IFU
System HELP
Training Material
Laboratory Protocol
State the Aspiration Volume  Direct Observation
 Test   
 Other __________
Process a Bar Coded Sample (Cassette  Direct Observation
Presentation)  Test   
 Other __________
Process a Bar Coded Sample (Single Tube  Direct Observation
Presentation)  Test   
 Other __________
Process a STAT  Direct Observation
 Test   
 Other __________
Process a Sample in a microtainer using  Direct Observation
Single tube Presentation  Test   
 Other __________
Rev. A (June 2014) 111 of 118

TROUBLESHOOTING IFU
System HELP
Training Material
Removing System Covers  Direct Observation
 Test   
 Other __________
Components and Sample Flow  Direct Observation
 Test   
 Other __________
Replacement Procedures  Direct Observation
 Test   
 Other __________
Cleaning Procedures  Direct Observation
 Test   
 Other __________
Using Diagnostics  Direct Observation
 Test   
 Other __________
Optimizing Stain Protocols  Direct Observation
Smear Quality and Stain Quality  Test   
 Other __________
ADDITIONAL TOPICS IFU
System HELP
Training Material
History Logs  Direct Observation
 Test   
 Other __________
Operator and Rules  Direct Observation
 Test   
 Other __________
Configure Auto Stop Settings  Direct Observation
 Test   
 Other __________
Enabling Batching  Direct Observation
 Test   
 Other __________
Rev. A (June 2014) 112 of 118

UniCel® DxH SlideMaker Examples of Stain Protocols
1. Protocol Options (Customer)
Wright Giemsa with Quick Rinse Stain Protocol

Bath Supply Type % Duration
1 Methanol 100% 25 seconds
2 Stain 100% 2 minutes
3 Buffer/Stain Buffer 83%, Stain 17% 3 min, 15 seconds
4 Quick Rinse Water 75%, Methanol 25% 6 seconds
5 Water 100% 2 minutes
Auto drain and refill for bath 4 every 15 baskets

Drying Duration: 2 minutes
SMS Configuration>Basket Settings

1. Distribution: Every other position: 10
2. Stat Timeout: 5 minutes
3. ASAP Timeout: 8 minutes
4. Routine Timeout: 10 minutes
5. Pre-Staining Drying Time: 6 minute

2 Stain 100% 3 minutes
3 Buffer/Stain Buffer 90%, Stain 10% 3 minutes
5 Water 100% 2 min, 30 seconds

Drying Duration 3 minutes

5. Pre-Staining Drying Time: 6 minutes
Rev. A (June 2014) 113 of 118

Wright Stain Protocol

2 Stain 100% 1 min, 15 seconds
3 Buffer/Stain Buffer 95%, Stain 5% 2 minutes
4 Quick Rinse N/A N/A


5. Pre-Staining Drying Time: 6minutes

2 Stain 100% 1 min, 20 seconds
3 Buffer/Stain Buffer 90%, Stain 10% 2 min, 10 seconds


5. Pre-Staining Drying Time: 6 minutes
Rev. A (June 2014) 114 of 118

Beckman Coulter Website
Training & Education
(Accessing the Training Materials on the Web)
Step Action
Go to www.beckmancoulter.com.
1
If promted click on Americas, then click on the United States
Double click on Diagnostics.
Click on Training & Education.
Rev. A (June 2014) 115 of 118

Step Action
Click on Hematology.
Click on the Hematology product you want to view.
ie: UniCel® DxH 800.

UniCel ® DxH Slidemaker
Stainer, LH 750, LH780.
When you open the file to the product you will find 4 tabs:
Tools& Aids = This tab provides you with the training materials used for Customer
Operator Training.
On Demand = This tab provides you with different training solutions offering 24/7
6 access. (You must create an account to receive PACE and or a Florida CE Broker
certificates).
Webinars& Conferences = This tab provides web based learning conferences and
Virtual Symposia.
Instructor Lead = This tab provides information regarding the training curriculum.
Rev. A (June 2014) 116 of 118

NOTES:
Rev. A (June 2014) 117 of 118

NOTES:
Rev. A (June 2014) 118 of 118


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UniCel DxH Series

Coulter Cellular Analysis System

UniCel DxH Series Training Pocket Resources

WARNINGS AND PRECAUTIONS

INTENTION FOR USE

Copyright  Beckman Coulter, Inc. 2014

Find us on the World Wide Web at:

Beckman Coulter Ireland, Inc.

ii of vi UniCel DxH Series Training Pocket Resources

Revision A (June 2014)

The information contained in this manual has been reviewed and

UniCel DxH Pocket Resources

UniCel DxH 800 Training Resources

UniCel DxH Slidemaker Stainer Training Resources

UniCel DxH Series Training Pocket Resources iii of vi

iv of vi UniCel DxH Series Training Pocket Resources

TRAINING RESOURCES TOPICS

UNICEL DXH SERIES JOB AIDS

UNICEL DXH SERIES CLEANING PROCEDURE CHART

UNICEL DXH SERIES COMPETENCY CHECKLIST

EXAMPLES OF PATIENT RESULTS

UNICEL DXH SLIDEMAKER STAINER JOB AIDS

UNICEL DXH SLIDEMAKER STAINER CLEANING PROCEDURE

UNICEL DXH SLIDEMAKER STAINER COMPETENCY CHECKLIST

STEPS ON ACCESSING TRAINING MATERIALS ON THE WEB

UniCel DxH Series Training Pocket Resources v of vi

vi of vi UniCel DxH Series Training Pocket Resources

Rev. A (June 2014) 1 of 118

Rev. A (June 2014) 2 of 118

Rev. A (June 2014) 3 of 118

To Log Off from the System Manager

On the Log off screen, select the Log off button.

3 You are now logged off from the System Manager.

To Log On to the System Manager

2 Type in the Password (case sensitive).

3 Select the Log On button or Press Enter.

4 You are now logged on to the System Manager.

Rev. A (June 2014) 4 of 118

2. Navigation Icons 6. Alert Status Icons

3. Local Navigation Icons 7. Utility Icons

4. Status Bar 8. System Icons

Rev. A (June 2014) 5 of 118

1 Select the System HELP icon .

You may search for topics using:

3 To place topic in Favorites as a reference, select the Favorites tab.

4 Locate a specific topic of interest.

5 Then, select the “Add” button at the bottom of the display.

7 To delete a topic from Favorites, highlight it and select “Remove”.

11 To close Help, select “X”

Rev. A (June 2014) 6 of 118

Rev. A (June 2014) 7 of 118

Date/Time of Last Control Run Date/Time of Last

Rev. A (June 2014) 8 of 118

3 Select OK to run Daily Checks, or Select Cancel.

Rev. A (June 2014) 9 of 118