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    Liver Enzyme Lab Report - Dylon Freerksen

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    The student conducted a lab experiment to test how temperature and pH affect the activity of liver enzymes. They tested pieces of liver at ice cold, room temperature, warm, and boiling temperatures after adding hydrogen peroxide. The results showed the enzymes were most active at warm temperature. They also tested acid, base, and neutral pH conditions, finding the enzymes most active in basic conditions. In discussion, the student acknowledged their hypothesis that room temperature would be best was incorrect, and suggested issues with sample cleanliness could explain unexpected basic results.

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    Liver Enzyme Lab Report - Dylon Freerksen

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    api-593477553
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    The student conducted a lab experiment to test how temperature and pH affect the activity of liver enzymes. They tested pieces of liver at ice cold, room temperature, warm, and boiling temperatures after adding hydrogen peroxide. The results showed the enzymes were most active at warm temperature. They also tested acid, base, and neutral pH conditions, finding the enzymes most active in basic conditions. In discussion, the student acknowledged their hypothesis that room temperature would be best was incorrect, and suggested issues with sample cleanliness could explain unexpected basic results.

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    The student conducted a lab experiment to test how temperature and pH affect the activity of liver enzymes. They tested pieces of liver at ice cold, room temperature, warm, and boiling temperatures after adding hydrogen peroxide. The results showed the enzymes were most active at warm temperature. They also tested acid, base, and neutral pH conditions, finding the enzymes most active in basic conditions. In discussion, the student acknowledged their hypothesis that room temperature would be best was incorrect, and suggested issues with sample cleanliness could explain unexpected basic results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    97 views6 pages

    Liver Enzyme Lab Report - Dylon Freerksen

    Uploaded by

    api-593477553
    The student conducted a lab experiment to test how temperature and pH affect the activity of liver enzymes. They tested pieces of liver at ice cold, room temperature, warm, and boiling temperatures after adding hydrogen peroxide. The results showed the enzymes were most active at warm temperature. They also tested acid, base, and neutral pH conditions, finding the enzymes most active in basic conditions. In discussion, the student acknowledged their hypothesis that room temperature would be best was incorrect, and suggested issues with sample cleanliness could explain unexpected basic results.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    The key takeaways are that this lab experiment tested the effect of temperature and pH on an enzyme's reaction rate using hydrogen peroxide and liver samples.

    The purpose of the experiment was to see what temperature an enzyme works best at and what acids and bases it works best at as well.

    The results showed that the enzyme worked best at a warm temperature of 80°C and had reaction ratings of 5, 2, 4 and 3 for warm, room temperature, ice cold and hot/boiling temperatures respectively.

    Liver Enzyme Lab

    Name: Dylon Freerksen

    Date: October 24, 2019.

    Period: Biology 5
    Introduction:

    In this lab we’re going to test whether an enzyme works best at room

    temperature, warm temperature, boiling temperature, or ice cold

    temperature. An enzyme is a catalyst and typically a protein that speeds

    up the rate of all chemical reactions. Enzymes are vital for life and

    enzymes help break down large molecules into smaller pieces so that

    they can be easily absorbed by the body. Other enzymes help bind two

    molecules together to produce new molecules. Enzymes work with a

    substrate at an activation site to get products, but that can change with i

    inhibitor that stops an enzyme from working (Castro, 2014).

    Purpose:

    The purpose of our lab was to see what temperature an enzyme works

    best at and what acids and bases it works best at as well.

    Materials:

    1. 4 test tubes

    2. Tweezers

    3. 250 ml beaker
    4. Hydrogen Peroxide

    5. Test tube rack

    6. Hot plate Ice

    7. 10 ml graduated cylinder

    8. Thermometer

    9. Liver

    Hypothesis:

    I think an enzyme works best at room temperature cause nothing will

    change about the enzymes if its not iced, warmed, or boiled.

    Procedure:

    1. Label four test tubes 1 through 4.

    2. Put a small piece of liver in the four test tubes.

    3. Put test tubes 1 and P1 into the ice water for 3 minutes.

    4. Put test tubes 3 and P3 into the warm water for 3 minutes.

    5. Pour 2 ml of hydrogen peroxide into test tube 2 and rate the reaction.

    6. Put 1 ml of water into test tube 4 and put it into the boiling water for 5

    minutes.
    7. Let test tube 4 cool down and while your doing that you can combine test

    tubes 1 and P1 and test tubes 3 and P3 and rate the reaction of each

    reaction.

    8. When test tube 4 is done cooling down put 2 ml of hydrogen peroxide

    and rate the reaction.

    9. Clean up materials and no liver should be put down the drain.

    Data/Results:

    Table 1

    Test Tube # Temperature Rating


    1 Ice Cold (0°C) 4
    2 Room Temp. (23°C) 2
    3 Warm (80°C) 5
    4 Hot/Boiling (100°C) 3

    Table 1 shows the temperature we tested the enzymes of the liver and

    the rate of the reaction based on how it reacted to the hydrogen

    peroxide.

    Table 2
    Test Tube Label Rating
    Acid 0
    Base 5
    Neutral 2

    Table 2 shows the way that the enzymes in the liver reacted to an Acid

    and a Base and just Neutral.

    Discussion:

    In my hypothesis when I said that the enzymes work best at room

    temperature I was wrong. The enzymes work best the Warm temperature

    and at a Base. Although, at the base the enzymes weren’t supposed to

    react with the hydrogen peroxide at all. This could’ve been cause Mr.

    Ferstein didn’t make sure it was clean enough so maybe we just had

    disinfection problems.

    Sources:

    ● Castro, Joseph. “How Do Enzymes Work?” LiveScience, Purch, 2014,

    www.livescience.com/45145-how-do-enzymes-work.html.

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