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    Experiment 1: ACIDS, BASES, AND BUFFERS: Post-Lab Activity

    Uploaded by

    Sam Estanoco
    1. The document describes two experiments involving acids, bases, and buffers. The first experiment tested the pH of various solutions using pH paper and found that milk, beer, soft drinks, vinegar, and 0.1M HCl were acidic, while distilled water and 1.0% NaCl were neutral. 2. The second experiment measured the change in pH of various solutions after adding a strong acid or base. It found that 0.1M NaOH showed the greatest change in pH and that 0.1M HCl and 0.1M CH3COOH showed the smallest changes. 3. Buffers are important for biological cells to maintain a pH between 7.2-7.6

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    © All Rights Reserved
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    Experiment 1: ACIDS, BASES, AND BUFFERS: Post-Lab Activity

    Uploaded by

    Sam Estanoco
    188 views4 pages
    1. The document describes two experiments involving acids, bases, and buffers. The first experiment tested the pH of various solutions using pH paper and found that milk, beer, soft drinks, vinegar, and 0.1M HCl were acidic, while distilled water and 1.0% NaCl were neutral. 2. The second experiment measured the change in pH of various solutions after adding a strong acid or base. It found that 0.1M NaOH showed the greatest change in pH and that 0.1M HCl and 0.1M CH3COOH showed the smallest changes. 3. Buffers are important for biological cells to maintain a pH between 7.2-7.6

    Original Title

    CHEM2212_ESTANOCO.SAMUEL_expt 1 AGRI

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    1. The document describes two experiments involving acids, bases, and buffers. The first experiment tested the pH of various solutions using pH paper and found that milk, beer, soft drinks, vinegar, and 0.1M HCl were acidic, while distilled water and 1.0% NaCl were neutral. 2. The second experiment measured the change in pH of various solutions after adding a strong acid or base. It found that 0.1M NaOH showed the greatest change in pH and that 0.1M HCl and 0.1M CH3COOH showed the smallest changes. 3. Buffers are important for biological cells to maintain a pH between 7.2-7.6

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    188 views4 pages

    Experiment 1: ACIDS, BASES, AND BUFFERS: Post-Lab Activity

    Uploaded by

    Sam Estanoco
    1. The document describes two experiments involving acids, bases, and buffers. The first experiment tested the pH of various solutions using pH paper and found that milk, beer, soft drinks, vinegar, and 0.1M HCl were acidic, while distilled water and 1.0% NaCl were neutral. 2. The second experiment measured the change in pH of various solutions after adding a strong acid or base. It found that 0.1M NaOH showed the greatest change in pH and that 0.1M HCl and 0.1M CH3COOH showed the smallest changes. 3. Buffers are important for biological cells to maintain a pH between 7.2-7.6

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    1

    Experiment 1: ACIDS, BASES, AND BUFFERS

    Score:

    ____________________________________________________________________________

    Last name Given name MI

    Estanoco Samuel M.
    Course/Year Date performed Date
    submitted

    Romana Dina
    Laboratory class schedule Laboratory Instructor/Professor

    POST-LAB ACTIVITY
    RESULTS

    Table 1. Determine of pH of different solutions using pH paper.

    Substance pH Inference

    Milk 6 Acidic

    Beer 4 Acidic

    Softdrinks 3 Acidic

    Distilled water 7 Neutral

    Vinegar 2 Acidic

    0.1M HCl 1.083 Acidic

    1.0% NaCl 7 Neutral

    Exploratory questions:

    1. Which of the following solutions in table 1 are acidic? Neutral? Why do you think they are
    basic, acidic, netural?
    2

    The following solution which enlisted as acidic are Milk, Beer, Softdrinks, Vinegar, and 0.1M

    HCl. The solution which is Neutral interference are the Distilled water and 1.0% NaCl.

    Table 2. Determination of the change in pH of the following solutions upon addition of


    strong acid/base

    Solutions pH Solutions pH Difference in pH


    added
    Distilled water 7 0.001 M NaOH 3.00 10
    Distilled water 7 0.001 HCl 3 10
    0.1M HCl 1.083 0.001 M HCl 4 5.083
    0.1M NaOH 13 0.001 NaOH 3 16
    0.1M CH3COOH 2.87 0.001 M NaOH 3.00 5.87
    0.1 CH3COONa 8.9 0.001 M HCl 4 12.9
    0.2M Acetate buffer 5 0.001 M NaOH 3.00 8
    0.2M Acetate buffer 5 0.001 M HCl 4 9
    0.1M NaH2PO4 6.68 0.001 M NaOH 3.00 9.68
    0.1 M Na2HPO4 9.6 0.001 M HCl 4 13.6
    0.2M Phosphate buffer 6.8 0.001 M NaOH 3.00 9.8
    0.2M Phosphate buffer 6.8 0.001 M HCl 4 10.8

    Exploratory questions:

    2. Which solution(s) in table 2 shows greater change in pH upon addition of the indicated
    solutions? What is/are the reasons(s) for the observed greater change in pH?

    The solution with a greater change with solution added is 0.1M NaOH. By the amount of each
    solution there’s a greater difference of each solution base to the ph level of solution.

    3. Which solution(s) in table 2 shows smaller change in pH upon addition of the indicated
    solutions? What is/are the reason(s) for the observed smaller change in pH?

    The 0.1M HCl and 0.1M CH3COOH are the solution with a smaller change within. Moreover, it is
    base through the ph level of solution.

    4. What is the pH of the biological cell? Why is buffer important to biological cells? What will
    happen to living things if the cellular fluids are not buffered? Why?
    3

    The ph level of biological cell is 7.2 to 7.6. When an acid or basic is supplied to the buffer system,
    buffers attenuate the pH change. Buffers are vital in biological systems because of their capacity to keep
    pH steady. While carbonic acid is an essential byproduct of this process, its presence is transient since
    carbonic acid is expelled from the body as carbon dioxide gas every time we breathe. Without this
    buffer mechanism, the pH in our bodies would fluctuate too much, causing us to perish.

    REFERENCES:

    https://courses.lumenlearning.com/wm-nmbiology1/chapter/buffers-ph-acids-and-
    bases/#:~:text=Buffers%20are%20solutions%20that%20moderate,to%20maintain%20constant
    %20pH%20conditions.

    https://courses.lumenlearning.com/wm-nmbiology1/chapter/buffers-ph-acids-and-
    bases/#:~:text=While%20carbonic%20acid%20is%20an,we%20would%20fail%20to%20survive.
    4

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